Rh6DG141600

Belongs to the class-I aminoacyl-tRNA synthetase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0000872
Physical Location & Seq
Forward (+)
20303179 .. 20305962
2784 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG141600.1

Sequence Viewer

Length: 714 bp
ATGACCTTATTGCATATCGTATCAAGTTTACTCCTCATCCGCACGCTGGAGACAAGTGGTCCACAAGTTACAATTATGCTCATTGCATTGTGGATTCTCTTGAGAATATTTACCCATTCGCTGTGCACTTTAGAATTTGAGACACATCGTGGTTCATACTACTGGCTATTGCATTCTCTGGGCCTATATCAACCACATGTTTGGGAATATTCAAGACTGAATGTCAGTAACACTATTATGTCGAAGCATAAGTTAAATCGACTAGTGACTGAAAATTGGGTTGATGGAATTGGAATCACCAGAAGTGATTTTAGTATGATTCATCTGAGTCGCCTCGAGTACCACATCAGAGAAGAGCTCAATAAAACAGCTCCTCACACAATGGTTGTGCTTGATCTTGTAAAGGTCGTCATTACCAACTGGAAAGATGACCTCATAAAGGACTGTGAAGCAAGAAAGTGGCCCGATTCTGAGGCGGAGTCCTACAAGGTTCCATTTTCCAAGGTGTTGTACATTGAGTGCTCCAATTTTCGCCTAAAGACTCAAAATACTACTTCGGACTTGCTCCTGGAGAATCTGCCCTGCTCAGACACGCATGTCCTATCAAGTGTGTGGATGCAAAGGATGTTTGTTTTAGCTGAGGATAATGAGACTGTCCTTGAGATACAGGTCGAGTATGATCAGGAGAAGAAGACAAAGCCAAAGGCTATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00970 ko01100 map00970 map01100
Pfam Domains
Protein Families
tRNA-synt_1c tRNA-synt_1c_C tRNA_synt_1c_R1 tRNA_synt_1c_R2

Protein Analysis

237

Amino Acids

27.72

Weight (kDa)

6.2

Isoelectric Point (pI)

34.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
tRNA-synt_1c PF00749 37 - 103 6.5e-16 tRNA synthetases class I (E and Q), catalytic domain
tRNA-synt_1c_C PF03950 124 - 181 2.7e-08 tRNA synthetases class I (E and Q), anti-codon binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 596
AciI CCGC 2 cut(s) 40, 476
AcsI RAATTY 1 cut(s) 134
AdeI CACNNNGTG 1 cut(s) 149
AfaI GTAC 2 cut(s) 341, 512
AfiI CCNNNNNNNGG 2 cut(s) 46, 439
AflIII ACRYGT 1 cut(s) 196
AgsI TTSAA 1 cut(s) 213
AhdI GACNNNNNGTC 2 cut(s) 57, 221
AhlI ACTAGT 1 cut(s) 262
AjnI CCWGG 1 cut(s) 567
AluBI AGCT 3 cut(s) 358, 371, 638
AluI AGCT 3 cut(s) 358, 371, 638
Alw21I GWGCWC 3 cut(s) 128, 360, 524
Alw26I GTCTC 3 cut(s) 44, 134, 644
Alw44I GTGCAC 1 cut(s) 124
Ama87I CYCGRG 1 cut(s) 335
AoxI GGCC 2 cut(s) 181, 461
ApaLI GTGCAC 1 cut(s) 124
ApoI RAATTY 1 cut(s) 134
AspS9I GGNCC 3 cut(s) 59, 181, 462
AsuHPI GGTGA 1 cut(s) 289
AvaI CYCGRG 1 cut(s) 335
AvaII GGWCC 1 cut(s) 59
BaeGI GKGCMC 1 cut(s) 128
BanII GRGCYC 1 cut(s) 360
BbsI GAAGAC 1 cut(s) 698
Bbv12I GWGCWC 3 cut(s) 128, 360, 524
BbvCI CCTCAGC 1 cut(s) 639
BccI CCATC 1 cut(s) 278
BciT130I CCWGG 1 cut(s) 569
BclI TGATCA 1 cut(s) 679
BcoDI GTCTC 3 cut(s) 44, 134, 644
BcuI ACTAGT 1 cut(s) 262
BfaI CTAG 1 cut(s) 263
Bme1390I CCNGG 1 cut(s) 569
Bme18I GGWCC 1 cut(s) 59
BmeRI GACNNNNNGTC 2 cut(s) 57, 221
BmeT110I CYCGRG 1 cut(s) 335
BmgT120I GGNCC 3 cut(s) 59, 181, 462
BmiI GGNNCC 1 cut(s) 492
BmrFI CCNGG 1 cut(s) 569
BmsI GCATC 1 cut(s) 606
BpiI GAAGAC 1 cut(s) 698
BplI GAGNNNNNCTC 2 cut(s) 342, 374
BpmI CTGGAG 2 cut(s) 68, 590
Bpu10I CCTNAGC 1 cut(s) 639
BpuEI CTTGAG 2 cut(s) 121, 680
BsaJI CCNNGG 1 cut(s) 501
Bsc4I CCNNNNNNNGG 2 cut(s) 46, 439
Bse1I ACTGG 2 cut(s) 167, 425
Bse3DI GCAATG 1 cut(s) 81
BseBI CCWGG 1 cut(s) 569
BseDI CCNNGG 1 cut(s) 501
BseGI GGATG 3 cut(s) 36, 621, 630
BseLI CCNNNNNNNGG 2 cut(s) 46, 439
BseMI GCAATG 1 cut(s) 81
BseMII CTCAG 4 cut(s) 317, 462, 600, 630
BseNI ACTGG 2 cut(s) 167, 425
BseRI GAGGAG 2 cut(s) 23, 363
BseSI GKGCMC 1 cut(s) 128
BshFI GGCC 2 cut(s) 183, 463
BsiHKAI GWGCWC 3 cut(s) 128, 360, 524
BsiHKCI CYCGRG 1 cut(s) 335
BslI CCNNNNNNNGG 2 cut(s) 46, 439
BsmAI GTCTC 3 cut(s) 44, 134, 644
BsmI GAATGC 1 cut(s) 172
BsnI GGCC 2 cut(s) 183, 463
BsoBI CYCGRG 1 cut(s) 335
Bsp1286I GDGCHC 3 cut(s) 128, 360, 524
Bsp1407I TGTACA 1 cut(s) 510
Bsp143I GATC 2 cut(s) 394, 679
BspACI CCGC 2 cut(s) 40, 476
BspANI GGCC 2 cut(s) 183, 463
BspCNI CTCAG 4 cut(s) 318, 463, 599, 631
BspLI GGNNCC 1 cut(s) 492
BspQI GCTCTTC 1 cut(s) 348
BsrDI GCAATG 1 cut(s) 81
BsrGI TGTACA 1 cut(s) 510
BsrI ACTGG 2 cut(s) 167, 425
BssECI CCNNGG 1 cut(s) 501
BssMI GATC 2 cut(s) 394, 679
BssT1I CCWWGG 1 cut(s) 501
Bst2UI CCWGG 1 cut(s) 569
Bst4CI ACNGT 2 cut(s) 446, 655
Bst6I CTCTTC 1 cut(s) 348
BstAUI TGTACA 1 cut(s) 510
BstC8I GCNNGC 1 cut(s) 44
BstDEI CTNAG 4 cut(s) 326, 471, 586, 639
BstENI CCTNNNNNAGG 1 cut(s) 437
BstF5I GGATG 3 cut(s) 36, 621, 630
BstKTI GATC 2 cut(s) 397, 682
BstMAI GTCTC 3 cut(s) 44, 134, 644
BstMBI GATC 2 cut(s) 394, 679
BstNI CCWGG 1 cut(s) 569
BstNSI RCATGY 2 cut(s) 200, 599
BstSCI CCNGG 1 cut(s) 567
BstSLI GKGCMC 1 cut(s) 128
BstV2I GAAGAC 1 cut(s) 698
BstXI CCANNNNNNTGG 1 cut(s) 201
BsuRI GGCC 2 cut(s) 183, 463
BtsCI GGATG 3 cut(s) 36, 621, 630
Cac8I GCNNGC 1 cut(s) 44
Cfr13I GGNCC 3 cut(s) 59, 181, 462
Csp6I GTAC 2 cut(s) 340, 511
CviAII CATG 2 cut(s) 197, 596
CviJI RGCY 8 cut(s) 166, 183, 358, 371, 463, 638, 700, 707
CviKI_1 RGCY 8 cut(s) 166, 183, 358, 371, 463, 638, 700, 707
CviQI GTAC 2 cut(s) 340, 511
DdeI CTNAG 4 cut(s) 326, 471, 586, 639
DpnI GATC 2 cut(s) 396, 681
DpnII GATC 2 cut(s) 394, 679
DraIII CACNNNGTG 1 cut(s) 149
DrdI GACNNNNNNGTC 1 cut(s) 596
DriI GACNNNNNGTC 2 cut(s) 57, 221
DseDI GACNNNNNNGTC 1 cut(s) 596
Eam1104I CTCTTC 1 cut(s) 348
Eam1105I GACNNNNNGTC 2 cut(s) 57, 221
EarI CTCTTC 1 cut(s) 348
EciI GGCGGA 1 cut(s) 491
Ecl136II GAGCTC 1 cut(s) 358
Eco130I CCWWGG 1 cut(s) 501
Eco24I GRGCYC 1 cut(s) 360
Eco47I GGWCC 1 cut(s) 59
Eco53kI GAGCTC 1 cut(s) 358
Eco88I CYCGRG 1 cut(s) 335
EcoICRI GAGCTC 1 cut(s) 358
EcoNI CCTNNNNNAGG 1 cut(s) 437
EcoRII CCWGG 1 cut(s) 567
EcoT14I CCWWGG 1 cut(s) 501
EcoT38I GRGCYC 1 cut(s) 360
ErhI CCWWGG 1 cut(s) 501
FaeI CATG 2 cut(s) 200, 599
FatI CATG 2 cut(s) 196, 595
FbaI TGATCA 1 cut(s) 679
FokI GGATG 3 cut(s) 23, 628, 637
FriOI GRGCYC 1 cut(s) 360
FspBI CTAG 1 cut(s) 263
GsuI CTGGAG 2 cut(s) 68, 590
HaeIII GGCC 2 cut(s) 183, 463
Hin1II CATG 2 cut(s) 200, 599
HinfI GANTC 8 cut(s) 94, 294, 319, 328, 467, 479, 541, 574
HphI GGTGA 1 cut(s) 289
Hpy166II GTNNAC 3 cut(s) 29, 62, 126
Hpy188I TCNGA 5 cut(s) 327, 350, 472, 559, 589
Hpy188III TCNNGA 3 cut(s) 100, 213, 683
Hpy8I GTNNAC 3 cut(s) 29, 62, 126
HpyCH4III ACNGT 2 cut(s) 446, 655
HpyCH4V TGCA 5 cut(s) 13, 86, 126, 172, 619
HpyF3I CTNAG 4 cut(s) 326, 471, 586, 639
Hsp92II CATG 2 cut(s) 200, 599
Ksp22I TGATCA 1 cut(s) 679
Kzo9I GATC 2 cut(s) 394, 679
LguI GCTCTTC 1 cut(s) 348
LmnI GCTCC 3 cut(s) 376, 527, 570
LweI GCATC 1 cut(s) 606
MaeI CTAG 1 cut(s) 263
MaeIII GTNAC 3 cut(s) 67, 227, 265
MalI GATC 2 cut(s) 396, 681
MboI GATC 2 cut(s) 394, 679
MboII GAAGA 3 cut(s) 365, 700, 703
MhlI GDGCHC 3 cut(s) 128, 360, 524
MluCI AATT 5 cut(s) 72, 134, 274, 288, 526
MlyI GAGTC 3 cut(s) 337, 488, 535
MnlI CCTC 6 cut(s) 44, 344, 384, 443, 466, 634
MseI TTAA 1 cut(s) 254
MslI CAYNNNNRTG 1 cut(s) 236
MspR9I CCNGG 1 cut(s) 569
Mva1269I GAATGC 1 cut(s) 172
MvaI CCWGG 1 cut(s) 569
NdeII GATC 2 cut(s) 394, 679
NlaIII CATG 2 cut(s) 200, 599
NlaIV GGNNCC 1 cut(s) 492
NmuCI GTSAC 1 cut(s) 265
NspI RCATGY 2 cut(s) 200, 599
PaeR7I CTCGAG 1 cut(s) 335
PciI ACATGT 1 cut(s) 196
PciSI GCTCTTC 1 cut(s) 348
PctI GAATGC 1 cut(s) 172
PfeI GAWTC 5 cut(s) 94, 294, 319, 467, 574
PfoI TCCNGGA 1 cut(s) 567
PleI GAGTC 3 cut(s) 336, 487, 535
PpsI GAGTC 3 cut(s) 336, 487, 535
PscI ACATGT 1 cut(s) 196
Psp124BI GAGCTC 1 cut(s) 360
Psp6I CCWGG 1 cut(s) 567
PspGI CCWGG 1 cut(s) 567
PspN4I GGNNCC 1 cut(s) 492
PspPI GGNCC 3 cut(s) 59, 181, 462
PspXI VCTCGAGB 1 cut(s) 335
RsaI GTAC 2 cut(s) 341, 512
RsaNI GTAC 2 cut(s) 340, 511
RseI CAYNNNNRTG 1 cut(s) 236
SacI GAGCTC 1 cut(s) 360
SapI GCTCTTC 1 cut(s) 348
SaqAI TTAA 1 cut(s) 254
Sau3AI GATC 2 cut(s) 394, 679
Sau96I GGNCC 3 cut(s) 59, 181, 462
SchI GAGTC 3 cut(s) 337, 488, 535
ScrFI CCNGG 1 cut(s) 569
SduI GDGCHC 3 cut(s) 128, 360, 524
SetI ASST 9 cut(s) 8, 360, 373, 408, 435, 492, 507, 640, 672
SfaNI GCATC 1 cut(s) 606
Sfr274I CTCGAG 1 cut(s) 335
SinI GGWCC 1 cut(s) 59
SlaI CTCGAG 1 cut(s) 335
SmiMI CAYNNNNRTG 1 cut(s) 236
SmlI CTYRAG 3 cut(s) 100, 335, 659
SmoI CTYRAG 3 cut(s) 100, 335, 659
SpeI ACTAGT 1 cut(s) 262
Sse9I AATT 5 cut(s) 72, 134, 274, 288, 526
SsiI CCGC 2 cut(s) 40, 476
SspI AATATT 2 cut(s) 108, 209
SspMI CTAG 1 cut(s) 263
SstI GAGCTC 1 cut(s) 360
StyD4I CCNGG 1 cut(s) 567
StyI CCWWGG 1 cut(s) 501
TaaI ACNGT 2 cut(s) 446, 655
TaqI TCGA 4 cut(s) 242, 259, 336, 672
TasI AATT 5 cut(s) 72, 134, 274, 288, 526
TatI WGTACW 1 cut(s) 510
TfiI GAWTC 5 cut(s) 94, 294, 319, 467, 574
Tru1I TTAA 1 cut(s) 254
Tru9I TTAA 1 cut(s) 254
TseFI GTSAC 1 cut(s) 265
Tsp45I GTSAC 1 cut(s) 265
TspDTI ATGAA 2 cut(s) 144, 311
VneI GTGCAC 1 cut(s) 124
VpaK11BI GGWCC 1 cut(s) 59
XagI CCTNNNNNAGG 1 cut(s) 437
XapI RAATTY 1 cut(s) 134
XceI RCATGY 2 cut(s) 200, 599
XhoI CTCGAG 1 cut(s) 335
XspI CTAG 1 cut(s) 263
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.