Rw1G005360

Belongs to the class-I aminoacyl-tRNA synthetase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0000872
Physical Location & Seq
Forward (+)
10373603 .. 10375907
2305 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G005360.1

Sequence Viewer

Length: 2262 bp
ATGCCGGCCAAGGAGAAAACCCTTGAGCTCATGTTGAAGGTCGGACTGGACGAGCGGACGGCCAAAAACACAATGGCCAACAACAAGGTCACGTCCAATCTAAGGGCTGTCATTCACGAGGCTGCAGTTACTGATGGATGCAGTCGAAGCATTGGTAATCTTATATACACGGTTGCTACAAAGTTTCCAGCAAATGCCCTTGTGCATCGCCCGGTATTGGCACAGTACATTGTGTCTTCAAAGATTAAAACTCCAGCTCAGTTAGATGCGGCTTTATTATTTTTTGCAAATACGGGGCCAGAAAACTTCAAAACAAGTGAATTTGAAGAAGCATGTGGTGTTGGTGTTGAAGTTTCAGAAGAAGAAATCAAGCAATCTGTTAATGAGGTTTTTGAAGAGAATAAATCTGCAATTTTGGAGCAGCGCTATAAAACAAATGTGGGAAGCTTATTTGCACATCTTAGGAAGAGACATCCGTGGGCTGACCCAAAAATTGTCAAGGCATCCATTGATACAAAGTTGGGTGAATTACTTGGTGAAAGGACTGCAGCAGATGATGAAAAGGTTCCTAAAAACAAGAAAGAGAAACCTGCTAAAGTAGAGGAGAAAGCTACTGCTGAAGAAATTCTTAATCCATATGCGATATTCCCTCAACCAGAGGAAAACTTTAAGGTTCATACTTCAGTTTTCTTTAGTGATGGTTCTATTCTGAGATGTTGCAACTCAAAGGAACTCCTTGGAAAACACTTGAGCTTGACTGGGGGGAGAGTTCTAACTCGCTTTCCTCCTGAACCGAATGGATATCTACATATAGGCCATGCCAAGGCAATGTTTATTGACTTTGGCCTGGCAAAAGAGAGAGGTGGTGACTGCTATCTGAGGTATGATGACACAAATCCGGAAGCTGAAAAGAAAGAATATATTGATCATATTCAGGAAATTGTCAGTTGGATGGGTTGGGAGCCCTACAAGATCACTTACACTAGTGATTACTTTCACGATCTGTATGAATTAGCAGTGGAGCTCATACGAAGGGGTCACGCTTACGTTGATCATCAGACGGGGGATGAGATAAAGGAGTACAGGGAGAAAAAAATGAACAGCCCTTGGAGGGACAGGCCAATTAAAGAGTCGCTAAAACTTTTTGAGGATATGAGGCGTGGCCTGATTGAAGAAGGGAAAGCAACTCTGAGAATGAAACAAGACATGCAGAATGATAATTTTAACATGTATGACCTTATTGCATATCGTATCAAGTTTACTCCTCATCCACATGCTGGAGACAGTTGGTGTATCTACCCCAGTTACGACTATGCTCATTGCATAAATTTGAGACACGGCCTATATCAACCACATGTTTGGGAATATTCAAGACTGAATGTCAGTAACACTGTTATGTCGAAACGTAAGTTAAATCGACTAGTGACTGAAAATTGGGTTGATGGTTGGGATGATCCACGCCTTATGACACTAGCTGGTTTACGACGTAGGGGCGTGACATCGACTGCTATTAATACGTTTGTTAGAGAAATTGGAATCACCAGAAGTGATTGTAGTATGATTCATATGAGTCGCCTCGAGTACCACATCAGAGAAGAGCTCAATAAAACAGCTCCTCGCACAATGGTTGTGCTTGATCCTGTAAAGGTCGTCATTACCAACTGGAAAGATGACCTCATAAAGGACTGTGAAGCAAGAAAGTGGCCCGATTCTGAGGCAGAGTCCTACAAGGTTCCAATTTCCAAGGTGTTGTACATTGAGCGCTCCGATTTTCGCCTTAAAGACTCAAAAGACTACTTCGGACTTGCTCCTGGAAAATCTGCTCTGCTCAGATACGCATGTCCTATCAAGTGTGTGGATGCAAAAGATGTTGTTTTAGCCGAGGATAATGAGACTATCCTTGAGATAAGGGTCGAGTATGATCCGGAGAAGAAGACAAAGCCAAAGGGGGTGCTGCATTGGGTCGCTGAACCTTCTCCGGGGATCGATCCACTAGAAGTGGAAGTCCGATTGTTTGACAGACTATTCAACTCAGAGAATCCTGCTGAGCTTGATGACTGGCTTTCTGATCTGAATGGAAAATCAAAAGTGGTAATACCAAATGCATTCGCTGTTCCTCAACTTGCAAAAGGTGTTGATGTTGGTGACAAGTTTCAGTTCGAAAGACTTGGCTATTTTGTGGTCGACAAAGATTCGACTCCTGAGAAGCTTGTGTTTAATCGTACGGTCACACTGCGAGATAGCTACAAGAATGGGAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00970 ko01100 map00970 map01100
Pfam Domains
Protein Families
tRNA-synt_1c tRNA-synt_1c_C tRNA_synt_1c_R1 tRNA_synt_1c_R2

Protein Analysis

753

Amino Acids

86.26

Weight (kDa)

6.51

Isoelectric Point (pI)

38.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
tRNA_synt_1c_R1 PF04558 6 - 163 3.5e-54 Glutaminyl-tRNA synthetase, non-specific RNA binding region part 1
tRNA-synt_1c PF00749 257 - 442 1.4e-75 tRNA synthetases class I (E and Q), catalytic domain
tRNA-synt_1c PF00749 449 - 535 2.9e-20 tRNA synthetases class I (E and Q), catalytic domain
tRNA-synt_1c_C PF03950 538 - 641 7.9e-18 tRNA synthetases class I (E and Q), anti-codon binding domain
tRNA-synt_1c_C2 PF20974 651 - 729 1.4e-16 tRNA synthetases class I (E and Q), anti-codon binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 598
AccB7I CCANNNNNTGG 1 cut(s) 1277
AccBSI CCGCTC 1 cut(s) 55
AccI GTMKAC 1 cut(s) 2184
AccIII TCCGGA 2 cut(s) 898, 1924
AciI CCGC 2 cut(s) 55, 269
AclWI GGATC 5 cut(s) 1448, 1631, 1916, 1982, 1991
AcoI YGGCCR 3 cut(s) 6, 60, 75
AcsI RAATTY 3 cut(s) 320, 624, 1327
AcuI CTGAAG 2 cut(s) 639, 666
AfaI GTAC 5 cut(s) 227, 1082, 1583, 1754, 2224
AfeI AGCGCT 2 cut(s) 425, 1763
AfiI CCNNNNNNNGG 7 cut(s) 102, 217, 823, 1111, 1277, 1681, 1979
AflIII ACRYGT 2 cut(s) 1227, 1354
AgsI TTSAA 9 cut(s) 37, 240, 310, 326, 350, 395, 1172, 1371, 2029
AhdI GACNNNNNGTC 1 cut(s) 1379
AhlI ACTAGT 2 cut(s) 983, 1420
AjiI CACGTC 1 cut(s) 93
AjnI CCWGG 2 cut(s) 846, 1810
Alw21I GWGCWC 3 cut(s) 30, 1026, 1602
Alw26I GTCTC 4 cut(s) 463, 1275, 1327, 1886
AlwI GGATC 5 cut(s) 1448, 1631, 1916, 1982, 1991
AlwNI CAGNNNCTG 1 cut(s) 131
Ama87I CYCGRG 1 cut(s) 1577
Aor13HI TCCGGA 2 cut(s) 898, 1924
Aor51HI AGCGCT 2 cut(s) 425, 1763
ApeKI GCWGC 4 cut(s) 122, 421, 548, 1954
ApoI RAATTY 3 cut(s) 320, 624, 1327
AseI ATTAAT 1 cut(s) 1512
Asp700I GAANNNNTTC 2 cut(s) 564, 624
AspLEI GCGC 2 cut(s) 426, 1764
AspS9I GGNCC 2 cut(s) 296, 1704
AsuC2I CCSGG 2 cut(s) 212, 1980
AsuHPI GGTGA 5 cut(s) 536, 548, 878, 1531, 2156
AsuII TTCGAA 1 cut(s) 2160
AvaI CYCGRG 1 cut(s) 1577
BaeI ACNNNNGTAYC 2 cut(s) 1825, 1858
BalI TGGCCA 1 cut(s) 77
BanII GRGCYC 4 cut(s) 30, 966, 1026, 1602
BauI CACGAG 1 cut(s) 116
BbsI GAAGAC 2 cut(s) 228, 1940
Bbv12I GWGCWC 3 cut(s) 30, 1026, 1602
BbvI GCAGC 4 cut(s) 109, 433, 560, 1941
BccI CCATC 4 cut(s) 128, 692, 946, 1436
BceAI ACGGC 2 cut(s) 75, 1354
BciT130I CCWGG 2 cut(s) 848, 1812
BclI TGATCA 2 cut(s) 925, 1051
BcnI CCSGG 2 cut(s) 212, 1980
BcoDI GTCTC 4 cut(s) 463, 1275, 1327, 1886
BcuI ACTAGT 2 cut(s) 983, 1420
BfaI CTAG 4 cut(s) 984, 1421, 1472, 1994
BfmI CTRYAG 2 cut(s) 123, 546
BfoI RGCGCY 2 cut(s) 427, 1765
BfuAI ACCTGC 1 cut(s) 598
BisI GCNGC 5 cut(s) 123, 270, 422, 549, 1955
BlpI GCTNAGC 1 cut(s) 2046
BlsI GCNGC 5 cut(s) 124, 271, 423, 550, 1956
Bme1390I CCNGG 4 cut(s) 212, 848, 1812, 1980
BmeRI GACNNNNNGTC 1 cut(s) 1379
BmeT110I CYCGRG 1 cut(s) 1577
BmgBI CACGTC 1 cut(s) 93
BmgT120I GGNCC 2 cut(s) 296, 1704
BmiI GGNNCC 4 cut(s) 297, 567, 963, 1734
BmrFI CCNGG 4 cut(s) 212, 848, 1812, 1980
BmrI ACTGGG 2 cut(s) 768, 1296
BmsI GCATC 5 cut(s) 128, 214, 256, 512, 1849
BmuI ACTGGG 2 cut(s) 768, 1296
BpiI GAAGAC 2 cut(s) 228, 1940
BplI GAGNNNNNCTC 2 cut(s) 1584, 1616
BpmI CTGGAG 2 cut(s) 237, 1299
Bpu1102I GCTNAGC 1 cut(s) 2046
Bpu14I TTCGAA 1 cut(s) 2160
BpuEI CTTGAG 3 cut(s) 44, 769, 1922
BpuMI CCSGG 2 cut(s) 212, 1980
Bsa29I ATCGAT 1 cut(s) 1986
BsaJI CCNNGG 8 cut(s) 9, 476, 736, 822, 1106, 1743, 1881, 1979
BsaWI WCCGGW 2 cut(s) 898, 1924
Bsc4I CCNNNNNNNGG 7 cut(s) 102, 217, 823, 1111, 1277, 1681, 1979
Bse118I RCCGGY 1 cut(s) 4
Bse1I ACTGG 5 cut(s) 51, 763, 1302, 1667, 2063
Bse3DI GCAATG 2 cut(s) 834, 1318
BseAI TCCGGA 2 cut(s) 898, 1924
BseBI CCWGG 2 cut(s) 848, 1812
BseCI ATCGAT 1 cut(s) 1986
BseDI CCNNGG 8 cut(s) 9, 476, 736, 822, 1106, 1743, 1881, 1979
BseGI GGATG 8 cut(s) 143, 472, 503, 957, 1072, 1267, 1456, 1864
BseLI CCNNNNNNNGG 7 cut(s) 102, 217, 823, 1111, 1277, 1681, 1979
BseMI GCAATG 2 cut(s) 834, 1318
BseMII CTCAG 9 cut(s) 272, 701, 869, 1181, 1704, 1843, 2037, 2046, 2193
BseNI ACTGG 5 cut(s) 51, 763, 1302, 1667, 2063
BseRI GAGGAG 3 cut(s) 617, 1254, 1605
BseXI GCAGC 4 cut(s) 109, 433, 560, 1941
BshVI ATCGAT 1 cut(s) 1986
BsiHKAI GWGCWC 3 cut(s) 30, 1026, 1602
BsiHKCI CYCGRG 1 cut(s) 1577
BsiSI CCGG 5 cut(s) 5, 212, 899, 1925, 1979
BsiWI CGTACG 1 cut(s) 2222
BslFI GGGAC 1 cut(s) 1127
BslI CCNNNNNNNGG 7 cut(s) 102, 217, 823, 1111, 1277, 1681, 1979
BsmAI GTCTC 4 cut(s) 463, 1275, 1327, 1886
BsmFI GGGAC 1 cut(s) 1127
BsmI GAATGC 1 cut(s) 2105
BsoBI CYCGRG 1 cut(s) 1577
Bsp119I TTCGAA 1 cut(s) 2160
Bsp1286I GDGCHC 4 cut(s) 30, 966, 1026, 1602
Bsp13I TCCGGA 2 cut(s) 898, 1924
Bsp1407I TGTACA 1 cut(s) 1752
Bsp1720I GCTNAGC 1 cut(s) 2046
BspACI CCGC 2 cut(s) 55, 269
BspCNI CTCAG 9 cut(s) 271, 702, 870, 1182, 1705, 1842, 2038, 2045, 2194
BspDI ATCGAT 1 cut(s) 1986
BspEI TCCGGA 2 cut(s) 898, 1924
BspLI GGNNCC 4 cut(s) 297, 567, 963, 1734
BspMAI CTGCAG 2 cut(s) 127, 550
BspMI ACCTGC 1 cut(s) 598
BspPI GGATC 5 cut(s) 1448, 1631, 1916, 1982, 1991
BspQI GCTCTTC 1 cut(s) 1590
BspT104I TTCGAA 1 cut(s) 2160
BsrBI CCGCTC 1 cut(s) 55
BsrDI GCAATG 2 cut(s) 834, 1318
BsrFI RCCGGY 1 cut(s) 4
BsrGI TGTACA 1 cut(s) 1752
BsrI ACTGG 5 cut(s) 51, 763, 1302, 1667, 2063
BssAI RCCGGY 1 cut(s) 4
BssECI CCNNGG 8 cut(s) 9, 476, 736, 822, 1106, 1743, 1881, 1979
BssSI CACGAG 1 cut(s) 116
BssT1I CCWWGG 5 cut(s) 9, 736, 822, 1106, 1743
Bst2BI CACGAG 1 cut(s) 116
Bst2UI CCWGG 2 cut(s) 848, 1812
Bst4CI ACNGT 6 cut(s) 172, 225, 1286, 1393, 1688, 2227
Bst6I CTCTTC 3 cut(s) 390, 461, 1590
BstAUI TGTACA 1 cut(s) 1752
BstBI TTCGAA 1 cut(s) 2160
BstC8I GCNNGC 1 cut(s) 6
BstDSI CCRYGG 1 cut(s) 476
BstENI CCTNNNNNAGG 1 cut(s) 1679
BstF5I GGATG 8 cut(s) 143, 472, 503, 957, 1072, 1267, 1456, 1864
BstH2I RGCGCY 2 cut(s) 427, 1765
BstHHI GCGC 2 cut(s) 426, 1764
BstMAI GTCTC 4 cut(s) 463, 1275, 1327, 1886
BstMWI GCNNNNNNNGC 1 cut(s) 147
BstNI CCWGG 2 cut(s) 848, 1812
BstNSI RCATGY 6 cut(s) 336, 1210, 1231, 1277, 1358, 1842
BstSCI CCNGG 4 cut(s) 210, 846, 1810, 1978
BstSFI CTRYAG 2 cut(s) 123, 546
BstV1I GCAGC 4 cut(s) 109, 433, 560, 1941
BstV2I GAAGAC 2 cut(s) 228, 1940
BstXI CCANNNNNNTGG 1 cut(s) 1359
Bsu15I ATCGAT 1 cut(s) 1986
BsuTUI ATCGAT 1 cut(s) 1986
BtgI CCRYGG 1 cut(s) 476
BtgZI GCGATG 1 cut(s) 191
BtrI CACGTC 1 cut(s) 93
BtsCI GGATG 8 cut(s) 143, 472, 503, 957, 1072, 1267, 1456, 1864
BtsI GCAGTG 2 cut(s) 1023, 2231
BtsIMutI CAGTG 3 cut(s) 1023, 1389, 2231
BveI ACCTGC 1 cut(s) 598
Cac8I GCNNGC 1 cut(s) 6
CaiI CAGNNNCTG 1 cut(s) 131
CfoI GCGC 2 cut(s) 426, 1764
Cfr10I RCCGGY 1 cut(s) 4
Cfr13I GGNCC 2 cut(s) 296, 1704
ClaI ATCGAT 1 cut(s) 1986
Csp6I GTAC 5 cut(s) 226, 1081, 1582, 1753, 2223
CviAII CATG 8 cut(s) 31, 333, 818, 1207, 1228, 1274, 1355, 1839
CviQI GTAC 5 cut(s) 226, 1081, 1582, 1753, 2223
DriI GACNNNNNGTC 1 cut(s) 1379
EaeI YGGCCR 3 cut(s) 6, 60, 75
Eam1104I CTCTTC 3 cut(s) 390, 461, 1590
Eam1105I GACNNNNNGTC 1 cut(s) 1379
EarI CTCTTC 3 cut(s) 390, 461, 1590
Ecl136II GAGCTC 3 cut(s) 28, 1024, 1600
Eco130I CCWWGG 5 cut(s) 9, 736, 822, 1106, 1743
Eco24I GRGCYC 4 cut(s) 30, 966, 1026, 1602
Eco32I GATATC 1 cut(s) 803
Eco47III AGCGCT 2 cut(s) 425, 1763
Eco53kI GAGCTC 3 cut(s) 28, 1024, 1600
Eco57I CTGAAG 2 cut(s) 639, 666
Eco88I CYCGRG 1 cut(s) 1577
EcoICRI GAGCTC 3 cut(s) 28, 1024, 1600
EcoNI CCTNNNNNAGG 1 cut(s) 1679
EcoRII CCWGG 2 cut(s) 846, 1810
EcoRV GATATC 1 cut(s) 803
EcoT14I CCWWGG 5 cut(s) 9, 736, 822, 1106, 1743
EcoT22I ATGCAT 1 cut(s) 2107
EcoT38I GRGCYC 4 cut(s) 30, 966, 1026, 1602
ErhI CCWWGG 5 cut(s) 9, 736, 822, 1106, 1743
FaeI CATG 8 cut(s) 34, 336, 821, 1210, 1231, 1277, 1358, 1842
FalI AAGNNNNNCTT 2 cut(s) 612, 644
FaqI GGGAC 1 cut(s) 1127
FatI CATG 8 cut(s) 30, 332, 817, 1206, 1227, 1273, 1354, 1838
FauNDI CATATG 2 cut(s) 637, 1566
FbaI TGATCA 2 cut(s) 925, 1051
FblI GTMKAC 1 cut(s) 2184
Fnu4HI GCNGC 5 cut(s) 123, 270, 422, 549, 1955
FokI GGATG 8 cut(s) 150, 459, 490, 964, 1079, 1254, 1463, 1871
FriOI GRGCYC 4 cut(s) 30, 966, 1026, 1602
Fsp4HI GCNGC 5 cut(s) 123, 270, 422, 549, 1955
FspBI CTAG 4 cut(s) 984, 1421, 1472, 1994
GlaI GCGC 2 cut(s) 425, 1763
GluI GCNGC 5 cut(s) 123, 270, 422, 549, 1955
GsuI CTGGAG 2 cut(s) 237, 1299
HaeII RGCGCY 2 cut(s) 427, 1765
HapII CCGG 5 cut(s) 5, 212, 899, 1925, 1979
HhaI GCGC 2 cut(s) 426, 1764
Hin1II CATG 8 cut(s) 34, 336, 821, 1210, 1231, 1277, 1358, 1842
Hin6I GCGC 2 cut(s) 424, 1762
HinP1I GCGC 2 cut(s) 424, 1762
HincII GTYRAC 1 cut(s) 2185
HindII GTYRAC 1 cut(s) 2185
HindIII AAGCTT 2 cut(s) 445, 2207
HpaII CCGG 5 cut(s) 5, 212, 899, 1925, 1979
HphI GGTGA 5 cut(s) 536, 548, 878, 1531, 2156
Hpy166II GTNNAC 3 cut(s) 1260, 1481, 2185
Hpy188III TCNNGA 8 cut(s) 116, 788, 899, 935, 998, 1371, 1925, 2201
Hpy8I GTNNAC 3 cut(s) 1260, 1481, 2185
Hpy99I CGWCG 1 cut(s) 1488
HpyAV CCTTC 4 cut(s) 31, 1026, 1169, 1983
HpyCH4III ACNGT 6 cut(s) 172, 225, 1286, 1393, 1688, 2227
HpyCH4IV ACGT 5 cut(s) 92, 1047, 1405, 1486, 1517
HpyF10VI GCNNNNNNNGC 1 cut(s) 147
HpySE526I ACGT 5 cut(s) 92, 1047, 1405, 1486, 1517
Hsp92II CATG 8 cut(s) 34, 336, 821, 1210, 1231, 1277, 1358, 1842
HspAI GCGC 2 cut(s) 424, 1762
Kpn2I TCCGGA 2 cut(s) 898, 1924
KroI GCCGGC 1 cut(s) 4
KroNI GCCGGC 1 cut(s) 6
Ksp22I TGATCA 2 cut(s) 925, 1051
LguI GCTCTTC 1 cut(s) 1590
LmnI GCTCC 6 cut(s) 418, 961, 1021, 1618, 1769, 1813
Lsp1109I GCAGC 4 cut(s) 109, 433, 560, 1941
LweI GCATC 5 cut(s) 128, 214, 256, 512, 1849
MaeI CTAG 4 cut(s) 984, 1421, 1472, 1994
MaeII ACGT 5 cut(s) 92, 1047, 1405, 1486, 1517
MbiI CCGCTC 1 cut(s) 55
MhlI GDGCHC 4 cut(s) 30, 966, 1026, 1602
MlsI TGGCCA 1 cut(s) 77
MluNI TGGCCA 1 cut(s) 77
MlyI GAGTC 5 cut(s) 1139, 1579, 1730, 1778, 2191
MmeI TCCRAC 2 cut(s) 22, 929
Mox20I TGGCCA 1 cut(s) 77
Mph1103I ATGCAT 1 cut(s) 2107
MroI TCCGGA 2 cut(s) 898, 1924
MroNI GCCGGC 1 cut(s) 4
MroXI GAANNNNTTC 2 cut(s) 564, 624
MscI TGGCCA 1 cut(s) 77
MslI CAYNNNNRTG 2 cut(s) 1272, 1394
Msp20I TGGCCA 1 cut(s) 77
MspI CCGG 5 cut(s) 5, 212, 899, 1925, 1979
MspR9I CCNGG 4 cut(s) 212, 848, 1812, 1980
Mva1269I GAATGC 1 cut(s) 2105
MvaI CCWGG 2 cut(s) 848, 1812
MwoI GCNNNNNNNGC 1 cut(s) 147
NaeI GCCGGC 1 cut(s) 6
NciI CCSGG 2 cut(s) 212, 1980
NdeI CATATG 2 cut(s) 637, 1566
NgoMIV GCCGGC 1 cut(s) 4
NlaIII CATG 8 cut(s) 34, 336, 821, 1210, 1231, 1277, 1358, 1842
NlaIV GGNNCC 4 cut(s) 297, 567, 963, 1734
NmeAIII GCCGAG 1 cut(s) 1906
NmuCI GTSAC 7 cut(s) 88, 866, 1037, 1423, 1495, 2144, 2227
NsiI ATGCAT 1 cut(s) 2107
NspI RCATGY 6 cut(s) 336, 1210, 1231, 1277, 1358, 1842
NspV TTCGAA 1 cut(s) 2160
PaeR7I CTCGAG 1 cut(s) 1577
PciI ACATGT 2 cut(s) 1227, 1354
PciSI GCTCTTC 1 cut(s) 1590
PcsI WCGNNNNNNNCGW 1 cut(s) 48
PctI GAATGC 1 cut(s) 2105
PdiI GCCGGC 1 cut(s) 6
PdmI GAANNNNTTC 2 cut(s) 564, 624
PfeI GAWTC 5 cut(s) 1536, 1561, 1709, 2038, 2192
Pfl23II CGTACG 1 cut(s) 2222
PflMI CCANNNNNTGG 1 cut(s) 1277
PfoI TCCNGGA 1 cut(s) 1810
PkrI GCNGC 5 cut(s) 124, 271, 423, 550, 1956
PleI GAGTC 5 cut(s) 1138, 1578, 1729, 1778, 2191
PpsI GAGTC 5 cut(s) 1138, 1578, 1729, 1778, 2191
PscI ACATGT 2 cut(s) 1227, 1354
PshBI ATTAAT 1 cut(s) 1512
Psp124BI GAGCTC 3 cut(s) 30, 1026, 1602
Psp6I CCWGG 2 cut(s) 846, 1810
PspGI CCWGG 2 cut(s) 846, 1810
PspLI CGTACG 1 cut(s) 2222
PspN4I GGNNCC 4 cut(s) 297, 567, 963, 1734
PspPI GGNCC 2 cut(s) 296, 1704
PspXI VCTCGAGB 1 cut(s) 1577
PstI CTGCAG 2 cut(s) 127, 550
PstNI CAGNNNCTG 1 cut(s) 131
RsaI GTAC 5 cut(s) 227, 1082, 1583, 1754, 2224
RsaNI GTAC 5 cut(s) 226, 1081, 1582, 1753, 2223
RseI CAYNNNNRTG 2 cut(s) 1272, 1394
SacI GAGCTC 3 cut(s) 30, 1026, 1602
SalI GTCGAC 1 cut(s) 2183
SapI GCTCTTC 1 cut(s) 1590
SatI GCNGC 5 cut(s) 123, 270, 422, 549, 1955
Sau96I GGNCC 2 cut(s) 296, 1704
SchI GAGTC 5 cut(s) 1139, 1579, 1730, 1778, 2191
ScrFI CCNGG 4 cut(s) 212, 848, 1812, 1980
SduI GDGCHC 4 cut(s) 30, 966, 1026, 1602
SfaNI GCATC 5 cut(s) 128, 214, 256, 512, 1849
SfcI CTRYAG 2 cut(s) 123, 546
Sfr274I CTCGAG 1 cut(s) 1577
SfuI TTCGAA 1 cut(s) 2160
SlaI CTCGAG 1 cut(s) 1577
SmiMI CAYNNNNRTG 2 cut(s) 1272, 1394
SmlI CTYRAG 4 cut(s) 23, 748, 1577, 1901
SmoI CTYRAG 4 cut(s) 23, 748, 1577, 1901
SpeI ACTAGT 2 cut(s) 983, 1420
SsiI CCGC 2 cut(s) 55, 269
SspI AATATT 1 cut(s) 1367
SspMI CTAG 4 cut(s) 984, 1421, 1472, 1994
SstI GAGCTC 3 cut(s) 30, 1026, 1602
StyD4I CCNGG 4 cut(s) 210, 846, 1810, 1978
StyI CCWWGG 5 cut(s) 9, 736, 822, 1106, 1743
TaaI ACNGT 6 cut(s) 172, 225, 1286, 1393, 1688, 2227
TaiI ACGT 5 cut(s) 95, 1050, 1408, 1489, 1520
TatI WGTACW 3 cut(s) 225, 1080, 1752
TauI GCSGC 1 cut(s) 272
TfiI GAWTC 5 cut(s) 1536, 1561, 1709, 2038, 2192
TscAI CASTG 3 cut(s) 1023, 1396, 2238
TseFI GTSAC 7 cut(s) 88, 866, 1037, 1423, 1495, 2144, 2227
TseI GCWGC 4 cut(s) 122, 421, 548, 1954
Tsp45I GTSAC 7 cut(s) 88, 866, 1037, 1423, 1495, 2144, 2227
TspDTI ATGAA 6 cut(s) 573, 665, 1023, 1112, 1211, 1553
TspGWI ACGGA 1 cut(s) 465
TspRI CASTG 3 cut(s) 1023, 1396, 2238
Van91I CCANNNNNTGG 1 cut(s) 1277
VspI ATTAAT 1 cut(s) 1512
XagI CCTNNNNNAGG 1 cut(s) 1679
XapI RAATTY 3 cut(s) 320, 624, 1327
XceI RCATGY 6 cut(s) 336, 1210, 1231, 1277, 1358, 1842
XcmI CCANNNNNNNNNTGG 1 cut(s) 70
XhoI CTCGAG 1 cut(s) 1577
XmiI GTMKAC 1 cut(s) 2184
XmnI GAANNNNTTC 2 cut(s) 564, 624
XspI CTAG 4 cut(s) 984, 1421, 1472, 1994
Zsp2I ATGCAT 1 cut(s) 2107
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.