Rroxscaffold_4G00325230

Belongs to the class-I aminoacyl-tRNA synthetase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0000872
Physical Location & Seq
Reverse (-)
56990632 .. 56991399
768 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00325230.1

Sequence Viewer

Length: 768 bp
ATGTCGAAGCGTAAGTTAAATCGACTAGTGACTGAAAATTGGGTTGATGGTTGGGATGATCCACGCCTTATGACACTAGCTGGTTTACGACGTAGGGGCGTGACATCGACTGCCATTAATAAGTTTGTTAGAGAAATTGGAATTACCAGAAGTGATTGTAGTATGATTCATCTGAGTCGCCTCGAGTACCACATCAGAGAAGAGCTCAATAAAACAGCTCCTCGCACAATGGTTGTGCTTGATCCTGTAAAGGTCGTCATTACCAACTGGAAAGATGACCTCATAAAGGACTGTGAAGCAAAAAAGTGGCCCGATTCTGAGGCAGAGTCCTACAAGGTTCCAATTTCGAAGGTGTTGTACATTGAGCGCTCCGATTTTCGCCTTAAAGACTCAAAAGACTACTTCGGACTTGCTCCTGGAAAATCTGCACTGCTCAGATACGCATGTCCTATCAAGTGTGTGGATGCAAAAGATGTTGTTTTAGCCGAGGATAATGAGACTGTCCTTGAGATAAGGGTCGAGTATGATCTGGAGAAGAAGACAAAGCCAAAGGGGGTGCTGCATTGGGTCGCTGAACCTTCTCCGGGGATTGATCCACTAGAAGTGGAAGTCCGATTGTTTGACAGACTATTCAACTCAGAGAATCCTGCTGAGCTTGATGACTGGCTTTCTGATCTGAATGGAAAATCAAAAGTGGTAATACCCAATGCATTTGCTGTTCCCCAACTTGCAAAAGGTGTTGATGTTGGTGACAAGTTTCAGTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00970 ko01100 map00970 map01100
Pfam Domains
Protein Families
tRNA-synt_1c tRNA-synt_1c_C tRNA_synt_1c_R1 tRNA_synt_1c_R2

Protein Analysis

255

Amino Acids

29.13

Weight (kDa)

6.37

Isoelectric Point (pI)

37.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
tRNA-synt_1c PF00749 1 - 70 1.2e-13 tRNA synthetases class I (E and Q), catalytic domain
tRNA-synt_1c_C PF03950 73 - 176 8.8e-19 tRNA synthetases class I (E and Q), anti-codon binding domain
tRNA-synt_1c_C2 PF20974 186 - 255 3.6e-10 tRNA synthetases class I (E and Q), anti-codon binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 3 cut(s) 53, 236, 587
AfaI GTAC 2 cut(s) 188, 359
AfeI AGCGCT 1 cut(s) 368
AfiI CCNNNNNNNGG 2 cut(s) 286, 584
AgsI TTSAA 1 cut(s) 634
AhlI ACTAGT 1 cut(s) 25
AjnI CCWGG 1 cut(s) 415
AluBI AGCT 4 cut(s) 80, 205, 218, 655
AluI AGCT 4 cut(s) 80, 205, 218, 655
Alw21I GWGCWC 1 cut(s) 207
Alw26I GTCTC 1 cut(s) 491
AlwI GGATC 3 cut(s) 53, 236, 587
Ama87I CYCGRG 1 cut(s) 182
Aor51HI AGCGCT 1 cut(s) 368
AoxI GGCC 1 cut(s) 308
ApeKI GCWGC 1 cut(s) 559
AseI ATTAAT 1 cut(s) 117
AspLEI GCGC 1 cut(s) 369
AspS9I GGNCC 1 cut(s) 309
AsuC2I CCSGG 1 cut(s) 585
AsuHPI GGTGA 1 cut(s) 761
AsuII TTCGAA 1 cut(s) 347
AvaI CYCGRG 1 cut(s) 182
BaeI ACNNNNGTAYC 2 cut(s) 430, 463
BanII GRGCYC 1 cut(s) 207
BarI GAAGNNNNNNTAC 2 cut(s) 341, 373
BbsI GAAGAC 1 cut(s) 545
Bbv12I GWGCWC 1 cut(s) 207
BbvI GCAGC 1 cut(s) 546
BccI CCATC 1 cut(s) 41
BciT130I CCWGG 1 cut(s) 417
BcnI CCSGG 1 cut(s) 585
BcoDI GTCTC 1 cut(s) 491
BcuI ACTAGT 1 cut(s) 25
BfaI CTAG 3 cut(s) 26, 77, 599
BfoI RGCGCY 1 cut(s) 370
BisI GCNGC 1 cut(s) 560
BlpI GCTNAGC 1 cut(s) 651
BlsI GCNGC 1 cut(s) 561
Bme1390I CCNGG 2 cut(s) 417, 585
BmeT110I CYCGRG 1 cut(s) 182
BmgT120I GGNCC 1 cut(s) 309
BmiI GGNNCC 1 cut(s) 339
BmrFI CCNGG 2 cut(s) 417, 585
BmsI GCATC 1 cut(s) 454
BpiI GAAGAC 1 cut(s) 545
BplI GAGNNNNNCTC 2 cut(s) 189, 221
BpmI CTGGAG 1 cut(s) 551
Bpu1102I GCTNAGC 1 cut(s) 651
Bpu14I TTCGAA 1 cut(s) 347
BpuEI CTTGAG 1 cut(s) 527
BpuMI CCSGG 1 cut(s) 585
BsaJI CCNNGG 2 cut(s) 486, 584
Bsc4I CCNNNNNNNGG 2 cut(s) 286, 584
Bse1I ACTGG 2 cut(s) 272, 668
BseBI CCWGG 1 cut(s) 417
BseDI CCNNGG 2 cut(s) 486, 584
BseGI GGATG 2 cut(s) 61, 469
BseLI CCNNNNNNNGG 2 cut(s) 286, 584
BseMII CTCAG 5 cut(s) 164, 309, 448, 642, 651
BseNI ACTGG 2 cut(s) 272, 668
BseRI GAGGAG 1 cut(s) 210
BseXI GCAGC 1 cut(s) 546
BsgI GTGCAG 1 cut(s) 411
BshFI GGCC 1 cut(s) 310
BsiHKAI GWGCWC 1 cut(s) 207
BsiHKCI CYCGRG 1 cut(s) 182
BsiSI CCGG 1 cut(s) 584
BslI CCNNNNNNNGG 2 cut(s) 286, 584
BsmAI GTCTC 1 cut(s) 491
BsnI GGCC 1 cut(s) 310
BsoBI CYCGRG 1 cut(s) 182
Bsp119I TTCGAA 1 cut(s) 347
Bsp1286I GDGCHC 1 cut(s) 207
Bsp1407I TGTACA 1 cut(s) 357
Bsp143I GATC 5 cut(s) 58, 241, 526, 592, 673
Bsp1720I GCTNAGC 1 cut(s) 651
BspANI GGCC 1 cut(s) 310
BspCNI CTCAG 5 cut(s) 165, 310, 447, 643, 650
BspLI GGNNCC 1 cut(s) 339
BspPI GGATC 3 cut(s) 53, 236, 587
BspQI GCTCTTC 1 cut(s) 195
BspT104I TTCGAA 1 cut(s) 347
BsrGI TGTACA 1 cut(s) 357
BsrI ACTGG 2 cut(s) 272, 668
BssECI CCNNGG 2 cut(s) 486, 584
BssMI GATC 5 cut(s) 58, 241, 526, 592, 673
Bst2UI CCWGG 1 cut(s) 417
Bst4CI ACNGT 2 cut(s) 293, 502
Bst6I CTCTTC 1 cut(s) 195
BstAUI TGTACA 1 cut(s) 357
BstBI TTCGAA 1 cut(s) 347
BstDEI CTNAG 5 cut(s) 173, 318, 434, 637, 651
BstENI CCTNNNNNAGG 1 cut(s) 284
BstF5I GGATG 2 cut(s) 61, 469
BstH2I RGCGCY 1 cut(s) 370
BstHHI GCGC 1 cut(s) 369
BstKTI GATC 5 cut(s) 61, 244, 529, 595, 676
BstMAI GTCTC 1 cut(s) 491
BstMBI GATC 5 cut(s) 58, 241, 526, 592, 673
BstNI CCWGG 1 cut(s) 417
BstNSI RCATGY 1 cut(s) 447
BstSCI CCNGG 2 cut(s) 415, 583
BstV1I GCAGC 1 cut(s) 546
BstV2I GAAGAC 1 cut(s) 545
BsuRI GGCC 1 cut(s) 310
BtsCI GGATG 2 cut(s) 61, 469
BtsI GCAGTG 1 cut(s) 428
BtsIMutI CAGTG 1 cut(s) 428
CfoI GCGC 1 cut(s) 369
Cfr13I GGNCC 1 cut(s) 309
Csp6I GTAC 2 cut(s) 187, 358
CviAII CATG 1 cut(s) 444
CviJI RGCY 8 cut(s) 80, 205, 218, 310, 485, 547, 655, 667
CviKI_1 RGCY 8 cut(s) 80, 205, 218, 310, 485, 547, 655, 667
CviQI GTAC 2 cut(s) 187, 358
DdeI CTNAG 5 cut(s) 173, 318, 434, 637, 651
DpnI GATC 5 cut(s) 60, 243, 528, 594, 675
DpnII GATC 5 cut(s) 58, 241, 526, 592, 673
Eam1104I CTCTTC 1 cut(s) 195
EarI CTCTTC 1 cut(s) 195
Ecl136II GAGCTC 1 cut(s) 205
Eco24I GRGCYC 1 cut(s) 207
Eco47III AGCGCT 1 cut(s) 368
Eco53kI GAGCTC 1 cut(s) 205
Eco88I CYCGRG 1 cut(s) 182
EcoICRI GAGCTC 1 cut(s) 205
EcoNI CCTNNNNNAGG 1 cut(s) 284
EcoRII CCWGG 1 cut(s) 415
EcoT22I ATGCAT 1 cut(s) 712
EcoT38I GRGCYC 1 cut(s) 207
FaeI CATG 1 cut(s) 447
FaiI YATR 5 cut(s) 71, 164, 284, 445, 525
FatI CATG 1 cut(s) 443
Fnu4HI GCNGC 1 cut(s) 560
FokI GGATG 2 cut(s) 68, 476
FriOI GRGCYC 1 cut(s) 207
Fsp4HI GCNGC 1 cut(s) 560
FspBI CTAG 3 cut(s) 26, 77, 599
GlaI GCGC 1 cut(s) 368
GluI GCNGC 1 cut(s) 560
GsuI CTGGAG 1 cut(s) 551
HaeII RGCGCY 1 cut(s) 370
HaeIII GGCC 1 cut(s) 310
HapII CCGG 1 cut(s) 584
HhaI GCGC 1 cut(s) 369
Hin1II CATG 1 cut(s) 447
Hin6I GCGC 1 cut(s) 367
HinP1I GCGC 1 cut(s) 367
HinfI GANTC 6 cut(s) 166, 175, 314, 326, 389, 643
HpaII CCGG 1 cut(s) 584
HphI GGTGA 1 cut(s) 761
Hpy166II GTNNAC 1 cut(s) 86
Hpy188III TCNNGA 1 cut(s) 530
Hpy8I GTNNAC 1 cut(s) 86
Hpy99I CGWCG 1 cut(s) 93
HpyAV CCTTC 2 cut(s) 343, 588
HpyCH4III ACNGT 2 cut(s) 293, 502
HpyCH4IV ACGT 1 cut(s) 91
HpyCH4V TGCA 5 cut(s) 428, 467, 562, 710, 731
HpyF3I CTNAG 5 cut(s) 173, 318, 434, 637, 651
HpySE526I ACGT 1 cut(s) 91
Hsp92II CATG 1 cut(s) 447
HspAI GCGC 1 cut(s) 367
Kzo9I GATC 5 cut(s) 58, 241, 526, 592, 673
LguI GCTCTTC 1 cut(s) 195
LmnI GCTCC 3 cut(s) 223, 374, 418
Lsp1109I GCAGC 1 cut(s) 546
LweI GCATC 1 cut(s) 454
MaeI CTAG 3 cut(s) 26, 77, 599
MaeII ACGT 1 cut(s) 91
MaeIII GTNAC 3 cut(s) 28, 100, 749
MalI GATC 5 cut(s) 60, 243, 528, 594, 675
MboI GATC 5 cut(s) 58, 241, 526, 592, 673
MboII GAAGA 3 cut(s) 212, 547, 550
MhlI GDGCHC 1 cut(s) 207
MluCI AATT 4 cut(s) 37, 135, 141, 342
MlyI GAGTC 3 cut(s) 184, 335, 383
MnlI CCTC 5 cut(s) 191, 231, 290, 313, 481
Mph1103I ATGCAT 1 cut(s) 712
MseI TTAA 3 cut(s) 17, 117, 384
MspI CCGG 1 cut(s) 584
MspR9I CCNGG 2 cut(s) 417, 585
MvaI CCWGG 1 cut(s) 417
NciI CCSGG 1 cut(s) 585
NdeII GATC 5 cut(s) 58, 241, 526, 592, 673
NlaIII CATG 1 cut(s) 447
NlaIV GGNNCC 1 cut(s) 339
NmeAIII GCCGAG 1 cut(s) 511
NmuCI GTSAC 3 cut(s) 28, 100, 749
NsiI ATGCAT 1 cut(s) 712
NspI RCATGY 1 cut(s) 447
NspV TTCGAA 1 cut(s) 347
PaeR7I CTCGAG 1 cut(s) 182
PciSI GCTCTTC 1 cut(s) 195
PfeI GAWTC 3 cut(s) 166, 314, 643
PfoI TCCNGGA 1 cut(s) 415
PkrI GCNGC 1 cut(s) 561
PleI GAGTC 3 cut(s) 183, 334, 383
PpsI GAGTC 3 cut(s) 183, 334, 383
PshBI ATTAAT 1 cut(s) 117
Psp124BI GAGCTC 1 cut(s) 207
Psp6I CCWGG 1 cut(s) 415
PspGI CCWGG 1 cut(s) 415
PspN4I GGNNCC 1 cut(s) 339
PspPI GGNCC 1 cut(s) 309
PspXI VCTCGAGB 1 cut(s) 182
RsaI GTAC 2 cut(s) 188, 359
RsaNI GTAC 2 cut(s) 187, 358
SacI GAGCTC 1 cut(s) 207
SapI GCTCTTC 1 cut(s) 195
SaqAI TTAA 3 cut(s) 17, 117, 384
SatI GCNGC 1 cut(s) 560
Sau3AI GATC 5 cut(s) 58, 241, 526, 592, 673
Sau96I GGNCC 1 cut(s) 309
SchI GAGTC 3 cut(s) 184, 335, 383
ScrFI CCNGG 2 cut(s) 417, 585
SduI GDGCHC 1 cut(s) 207
SfaNI GCATC 1 cut(s) 454
Sfr274I CTCGAG 1 cut(s) 182
SfuI TTCGAA 1 cut(s) 347
SlaI CTCGAG 1 cut(s) 182
SmlI CTYRAG 2 cut(s) 182, 506
SmoI CTYRAG 2 cut(s) 182, 506
SpeI ACTAGT 1 cut(s) 25
Sse9I AATT 4 cut(s) 37, 135, 141, 342
SspMI CTAG 3 cut(s) 26, 77, 599
SstI GAGCTC 1 cut(s) 207
StyD4I CCNGG 2 cut(s) 415, 583
TaaI ACNGT 2 cut(s) 293, 502
TaiI ACGT 1 cut(s) 94
TaqI TCGA 6 cut(s) 5, 22, 107, 183, 347, 519
TasI AATT 4 cut(s) 37, 135, 141, 342
TatI WGTACW 1 cut(s) 357
TfiI GAWTC 3 cut(s) 166, 314, 643
Tru1I TTAA 3 cut(s) 17, 117, 384
Tru9I TTAA 3 cut(s) 17, 117, 384
TscAI CASTG 1 cut(s) 435
TseFI GTSAC 3 cut(s) 28, 100, 749
TseI GCWGC 1 cut(s) 559
Tsp45I GTSAC 3 cut(s) 28, 100, 749
TspDTI ATGAA 1 cut(s) 158
TspRI CASTG 1 cut(s) 435
VspI ATTAAT 1 cut(s) 117
XagI CCTNNNNNAGG 1 cut(s) 284
XceI RCATGY 1 cut(s) 447
XhoI CTCGAG 1 cut(s) 182
XspI CTAG 3 cut(s) 26, 77, 599
Zsp2I ATGCAT 1 cut(s) 712
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.