MD16G1040700.v1.1

Belongs to the class-I aminoacyl-tRNA synthetase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0000872
Physical Location & Seq
Forward (+)
2887137 .. 2894182
7046 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1040700.v1.1.491

Sequence Viewer

Length: 2394 bp
ATGCCCGGCAAGGACGAGGGCGGCTCGTCCTCCGAGAAGGAGAAGAACCTCGAGCTCTTCCTGAAGGTCGGATTGGACGAGCGAACGGCCAAAAACACCATCGCCAACAACAAAGTCACCGCCAATCTCACCGCCGTCATTCACGAGGCGGCGGTTGCTGATGGATGCAGTCGAACAGTTGGTAATCTCCTCTACACGGTTGCCACGAAGCACCCAGCAAATGCCCTTGTGCATCGCCCAAAATTGGCGCAATACATTGTATTGTCGAAGATTAAAACCCCTGCCCAGTTAGAAGCAGCTTTTTCATTTTTTGCAACGACGGGGCCAGAGAATTTTGATATAAATAAATTTGAAGAAGCTTGTGGTGTTGGTGTTGAAGTTTCCGAAGAAGAAATAAAACAAACTGTTAATGAGGTTTTTGAGGAGAATAAGACTGCAATTTTGGAGCAGCGGTATAGAACAAATGTGGGTGATTTGTTTGCACACGTTAGGAAGAGGCATCCGTGGGCTGATCCAAAAATTGTAAAGCAATTTATTGATGCAAAATTGCGTGAATTACTTGGTGAAAGGACAGCAGCAGATGATGAGAAGGTTCCAAAAAAGAAGAAAGAGAAGCCTGCTAAAGTAGAGGAAAAAGTTGTTGCTGTTTCTACTCCAGAACAGCCATCTGAAGAAGTTCTTAATCCATACTCGATATTCCCTCAGCCATCGGATAATTATAAGGTTCATACTTCTGTTTTCTTTAGTGATGGTTCTATTTTGAGATGTTCCAATACAAGGGAATTGCTTGAGAAACACTTAAGCAGGACCGGAGGGAAAGTTTTGACCCGCTTTCCTCCTGAACCAAATGGGTATCTACATATAGGTCATGCCAAGGCAATGTTTATTGACTTTGGCCTGGCAAAAGAGAGGGATGGAGGCTGCTACCTACGGTTTGACGATACAAACCCAGAAGCTGAAAAGAAAGAATATATTGATCATATACAAGAAATCGTGAAGTGGATGGGTTGGGAGCCCTACAAGATTACTTACACCAGTGATTACTTTCAAGATCTGTACGATTTAGCAGTGGAACTCATACGAAGAGGTCATGCTTATGTTGATCATCAGACAGCGGATGAGATAAAGGAGTACAGGGAGAAAAAAATGAACAGCCCTTGGCGGGACAGGCCAATTGCAGAGTCATTGAAACTTTTTGAGGATATGAGACGAGGCCTAATCGAAGAAGGCAAAGCAACCCTTAGAATGAAGCAAGACATGCAGAGTGATAATTATAACATGTATGACCTTATTGCATATCGTATCAAGTTTACACCCCATCCGCATGCTGGAGACAAGTGGTGTATCTATCCAAGTTATGATTATGCTCACTGCATTGTTGATTCTCTTGAGGATATCACACATTCGCTTTGCACACTTGAATTTGAGACTCGCCGTGCTTCGTACTACTGGTTATTAAATGCCCTCGGACTTTATCAGCCATATGTATGGGAATATTCACGACTGAATGTCAGTAACACTGTCATGTCAAAGCGTAAGTTAAATCGACTAGTGACACAGAACTGGGTTGATGGTTGGGATGACCCACGTCTTATGACACTGGCTGGTCTACGGCGTAGGGGTGTTACTTCAACAGCAATAAATGCTTTTGTTAGAGGAATTGGAATCACTAGAAGTGATTGTAGTATGATACATTTAAGTCGACTTGAGTATCACATAAGAGAGGAGCTAAATAAAACAGCTCCTCGCACGATAGCTGTACTACATCCCCTCAAGGTGGTTATTACAAACCTGGAAGCTGGCACTATAATGGATCTAGAAGCAAGGAAGTGGCCTGATGCTCAAGCAGATGATGCATCTGCTTTCTACAAGGTTCCCTTTTCCAATACTGTTTATATTGACCAGTCTGATTTTCGACTTAAAGATTCAAAGGATTACTTTGGGCTTGCTCCTGGTAAATCTGCCCTGCTCAGATATGCGTTCCCTATTAAGTGCACGGATGTCATATTAGCTGATGATAAGGAAACTGTTCTTGAGATACGAGCTGAGTATGATCCTACAAAGAAGACAAAGCCAAAGGGGGTGCTTCACTGGGTTTCTGAACCTTCCCCAGGTGTTGATCCACTCAAGGTGGAAATCAGATTGTTTGACCGACTGTTCCTTTCAGAGAATCCTGCTGAGCTCGAAGACTGGCTTGCTGATTTGAACCCACAATCCAAGGTGATAATACCCAATGCATATGCTGTGCCCTCACTTGGAAGTGCTGTTGTTGGGGACACTTTCCAGTTTGAAAGGCTCGGATATTTTGTAGTTGACAAGGATTCAACTCCTGAGAAGCTCGTGTTCAATCGCACTGTCACGCTGAGAGATAGCTATCGCAAGGGCGGGATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00970 ko01100 map00970 map01100
Pfam Domains
Protein Families
tRNA-synt_1c tRNA-synt_1c_C tRNA_synt_1c_R1 tRNA_synt_1c_R2

Protein Analysis

798

Amino Acids

91.0

Weight (kDa)

6.33

Isoelectric Point (pI)

37.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
tRNA_synt_1c_R1 PF04558 15 - 172 9.1e-55 Glutaminyl-tRNA synthetase, non-specific RNA binding region part 1
tRNA_synt_1c_R2 PF04557 175 - 250 7.5e-07 Glutaminyl-tRNA synthetase, non-specific RNA binding region part 2
tRNA-synt_1c PF00749 273 - 578 1.3e-122 tRNA synthetases class I (E and Q), catalytic domain
tRNA-synt_1c_C PF03950 581 - 685 8.5e-18 tRNA synthetases class I (E and Q), anti-codon binding domain
tRNA-synt_1c_C2 PF20974 695 - 772 2.6e-18 tRNA synthetases class I (E and Q), anti-codon binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 720, 1275
AccI GTMKAC 2 cut(s) 1609, 1702
AclWI GGATC 4 cut(s) 506, 1821, 2048, 2114
AcoI YGGCCR 1 cut(s) 87
AcsI RAATTY 3 cut(s) 331, 347, 1421
AcuI CTGAAG 2 cut(s) 83, 690
AfaI GTAC 4 cut(s) 1058, 1133, 1445, 1761
AfiI CCNNNNNNNGG 8 cut(s) 196, 244, 777, 1162, 1328, 1777, 2111, 2255
AflII CTTAAG 1 cut(s) 799
AflIII ACRYGT 2 cut(s) 484, 1278
AhdI GACNNNNNGTC 1 cut(s) 1508
AhlI ACTAGT 1 cut(s) 1549
AjiI CACGTC 1 cut(s) 1589
AjnI CCWGG 4 cut(s) 897, 1791, 1951, 2110
AjuI GAANNNNNNNTTGG 2 cut(s) 56, 88
AloI GAACNNNNNNTCC 2 cut(s) 2198, 2230
Alw21I GWGCWC 3 cut(s) 57, 1997, 2184
Alw26I GTCTC 3 cut(s) 1201, 1326, 1421
Alw44I GTGCAC 1 cut(s) 1993
AlwI GGATC 4 cut(s) 506, 1821, 2048, 2114
AlwNI CAGNNNCTG 1 cut(s) 956
Ama87I CYCGRG 1 cut(s) 50
AoxI GGCC 6 cut(s) 87, 323, 895, 1169, 1213, 1832
ApaLI GTGCAC 1 cut(s) 1993
ApeKI GCWGC 4 cut(s) 296, 448, 575, 921
ApoI RAATTY 3 cut(s) 331, 347, 1421
ArsI GACNNNNNNTTYG 2 cut(s) 424, 456
Asp700I GAANNNNTTC 2 cut(s) 675, 2028
AspLEI GCGC 1 cut(s) 250
AspS9I GGNCC 2 cut(s) 323, 807
AsuC2I CCSGG 1 cut(s) 6
AsuHPI GGTGA 5 cut(s) 109, 121, 482, 575, 2233
AvaI CYCGRG 1 cut(s) 50
AvaII GGWCC 1 cut(s) 807
BaeGI GKGCMC 2 cut(s) 1997, 2250
BaeI ACNNNNGTAYC 2 cut(s) 1694, 1727
BanII GRGCYC 3 cut(s) 57, 1017, 2184
BauI CACGAG 2 cut(s) 143, 2339
BbsI GAAGAC 2 cut(s) 2072, 2193
Bbv12I GWGCWC 3 cut(s) 57, 1997, 2184
BbvCI CCTCAGC 1 cut(s) 702
BbvI GCAGC 4 cut(s) 308, 460, 587, 908
BccI CCATC 9 cut(s) 107, 155, 673, 715, 743, 908, 997, 1326, 1565
BceAI ACGGC 4 cut(s) 102, 119, 1419, 1628
BciT130I CCWGG 4 cut(s) 899, 1793, 1953, 2112
BclI TGATCA 2 cut(s) 976, 1102
BcnI CCSGG 1 cut(s) 6
BcoDI GTCTC 3 cut(s) 1201, 1326, 1421
BcuI ACTAGT 1 cut(s) 1549
BfaI CTAG 3 cut(s) 1550, 1671, 1817
BfrI CTTAAG 1 cut(s) 799
BglII AGATCT 1 cut(s) 1051
BisI GCNGC 6 cut(s) 22, 150, 297, 449, 576, 922
BlpI GCTNAGC 1 cut(s) 2178
BlsI GCNGC 6 cut(s) 23, 151, 298, 450, 577, 923
Bme1390I CCNGG 5 cut(s) 6, 899, 1793, 1953, 2112
Bme18I GGWCC 1 cut(s) 807
BmeRI GACNNNNNGTC 1 cut(s) 1508
BmeT110I CYCGRG 1 cut(s) 50
BmgBI CACGTC 1 cut(s) 1589
BmgT120I GGNCC 2 cut(s) 323, 807
BmiI GGNNCC 4 cut(s) 324, 594, 1014, 1875
BmrFI CCNGG 5 cut(s) 6, 899, 1793, 1953, 2112
BmrI ACTGGG 3 cut(s) 280, 1573, 2101
BmsI GCATC 7 cut(s) 155, 241, 508, 529, 1828, 1843, 1865
BmuI ACTGGG 3 cut(s) 280, 1573, 2101
BoxI GACNNNNGTC 1 cut(s) 1587
BpiI GAAGAC 2 cut(s) 2072, 2193
BplI GAGNNNNNCTC 2 cut(s) 8, 40
BpmI CTGGAG 2 cut(s) 639, 1350
Bpu10I CCTNAGC 1 cut(s) 702
Bpu1102I GCTNAGC 1 cut(s) 2178
BpuEI CTTGAG 7 cut(s) 809, 1409, 1727, 1757, 1827, 2054, 2111
BpuMI CCSGG 1 cut(s) 6
BsaBI GATNNNNATC 1 cut(s) 2373
BsaJI CCNNGG 6 cut(s) 503, 873, 1157, 1465, 2110, 2217
BsaWI WCCGGW 1 cut(s) 809
BsaXI ACNNNNNCTCC 4 cut(s) 437, 467, 804, 834
Bsc4I CCNNNNNNNGG 8 cut(s) 196, 244, 777, 1162, 1328, 1777, 2111, 2255
Bse1I ACTGG 9 cut(s) 286, 1035, 1454, 1568, 1605, 1903, 2096, 2195, 2284
Bse3DI GCAATG 1 cut(s) 885
Bse8I GATNNNNATC 1 cut(s) 2373
BseBI CCWGG 4 cut(s) 899, 1793, 1953, 2112
BseDI CCNNGG 6 cut(s) 503, 873, 1157, 1465, 2110, 2217
BseGI GGATG 9 cut(s) 170, 499, 919, 1008, 1123, 1318, 1585, 1765, 2005
BseJI GATNNNNATC 1 cut(s) 2373
BseLI CCNNNNNNNGG 8 cut(s) 196, 244, 777, 1162, 1328, 1777, 2111, 2255
BseMI GCAATG 1 cut(s) 885
BseMII CTCAG 6 cut(s) 716, 1984, 2037, 2169, 2322, 2354
BseNI ACTGG 9 cut(s) 286, 1035, 1454, 1568, 1605, 1903, 2096, 2195, 2284
BseRI GAGGAG 4 cut(s) 179, 437, 1734, 1739
BseSI GKGCMC 2 cut(s) 1997, 2250
BseXI GCAGC 4 cut(s) 308, 460, 587, 908
BseYI CCCAGC 1 cut(s) 214
BshFI GGCC 6 cut(s) 89, 325, 897, 1171, 1215, 1834
BsiHKAI GWGCWC 3 cut(s) 57, 1997, 2184
BsiHKCI CYCGRG 1 cut(s) 50
BsiSI CCGG 2 cut(s) 6, 810
BslFI GGGAC 2 cut(s) 1178, 2288
BslI CCNNNNNNNGG 8 cut(s) 196, 244, 777, 1162, 1328, 1777, 2111, 2255
BsmAI GTCTC 3 cut(s) 1201, 1326, 1421
BsmBI CGTCTC 1 cut(s) 1201
BsmFI GGGAC 2 cut(s) 1178, 2288
BsnI GGCC 6 cut(s) 89, 325, 897, 1171, 1215, 1834
BsoBI CYCGRG 1 cut(s) 50
Bsp1286I GDGCHC 5 cut(s) 57, 1017, 1997, 2184, 2250
Bsp143I GATC 7 cut(s) 511, 976, 1051, 1102, 1813, 2053, 2119
Bsp1720I GCTNAGC 1 cut(s) 2178
BspANI GGCC 6 cut(s) 89, 325, 897, 1171, 1215, 1834
BspCNI CTCAG 6 cut(s) 715, 1983, 2038, 2170, 2323, 2355
BspLI GGNNCC 4 cut(s) 324, 594, 1014, 1875
BspPI GGATC 4 cut(s) 506, 1821, 2048, 2114
BspQI GCTCTTC 1 cut(s) 62
BspTI CTTAAG 1 cut(s) 799
BsrDI GCAATG 1 cut(s) 885
BsrI ACTGG 9 cut(s) 286, 1035, 1454, 1568, 1605, 1903, 2096, 2195, 2284
BssECI CCNNGG 6 cut(s) 503, 873, 1157, 1465, 2110, 2217
BssMI GATC 7 cut(s) 511, 976, 1051, 1102, 1813, 2053, 2119
BssSI CACGAG 2 cut(s) 143, 2339
BssT1I CCWWGG 3 cut(s) 873, 1157, 2217
Bst2BI CACGAG 2 cut(s) 143, 2339
Bst2UI CCWGG 4 cut(s) 899, 1793, 1953, 2112
Bst4CI ACNGT 9 cut(s) 178, 199, 406, 933, 1522, 1891, 2029, 2157, 2356
Bst6I CTCTTC 3 cut(s) 62, 488, 1078
BstAFI CTTAAG 1 cut(s) 799
BstAPI GCANNNNNTGC 3 cut(s) 1258, 1643, 1853
BstC8I GCNNGC 5 cut(s) 618, 1326, 1801, 1947, 2196
BstDEI CTNAG 7 cut(s) 702, 1241, 1970, 2046, 2178, 2331, 2363
BstDSI CCRYGG 1 cut(s) 503
BstENI CCTNNNNNAGG 1 cut(s) 2109
BstF5I GGATG 9 cut(s) 170, 499, 919, 1008, 1123, 1318, 1585, 1765, 2005
BstHHI GCGC 1 cut(s) 250
BstKTI GATC 7 cut(s) 514, 979, 1054, 1105, 1816, 2056, 2122
BstMAI GTCTC 3 cut(s) 1201, 1326, 1421
BstMBI GATC 7 cut(s) 511, 976, 1051, 1102, 1813, 2053, 2119
BstMWI GCNNNNNNNGC 5 cut(s) 155, 1168, 1258, 1643, 1853
BstNI CCWGG 4 cut(s) 899, 1793, 1953, 2112
BstNSI RCATGY 3 cut(s) 1261, 1282, 1328
BstPAI GACNNNNGTC 1 cut(s) 1587
BstSCI CCNGG 5 cut(s) 4, 897, 1791, 1951, 2110
BstSLI GKGCMC 2 cut(s) 1997, 2250
BstV1I GCAGC 4 cut(s) 308, 460, 587, 908
BstV2I GAAGAC 2 cut(s) 2072, 2193
BstX2I RGATCY 2 cut(s) 1051, 1813
BstXI CCANNNNNNTGG 1 cut(s) 1488
BstYI RGATCY 2 cut(s) 1051, 1813
BsuRI GGCC 6 cut(s) 89, 325, 897, 1171, 1215, 1834
BtgI CCRYGG 1 cut(s) 503
BtgZI GCGATG 2 cut(s) 85, 218
BtrI CACGTC 1 cut(s) 1589
BtsCI GGATG 9 cut(s) 170, 499, 919, 1008, 1123, 1318, 1585, 1765, 2005
BtsI GCAGTG 2 cut(s) 1074, 1369
BtsIMutI CAGTG 7 cut(s) 1042, 1074, 1369, 1518, 1598, 2089, 2352
Cac8I GCNNGC 5 cut(s) 618, 1326, 1801, 1947, 2196
CaiI CAGNNNCTG 1 cut(s) 956
CfoI GCGC 1 cut(s) 250
Cfr13I GGNCC 2 cut(s) 323, 807
Csp6I GTAC 4 cut(s) 1057, 1132, 1444, 1760
CviAII CATG 6 cut(s) 869, 1091, 1258, 1279, 1325, 1525
CviQI GTAC 4 cut(s) 1057, 1132, 1444, 1760
DdeI CTNAG 7 cut(s) 702, 1241, 1970, 2046, 2178, 2331, 2363
DpnI GATC 7 cut(s) 513, 978, 1053, 1104, 1815, 2055, 2121
DpnII GATC 7 cut(s) 511, 976, 1051, 1102, 1813, 2053, 2119
DriI GACNNNNNGTC 1 cut(s) 1508
EaeI YGGCCR 1 cut(s) 87
Eam1104I CTCTTC 3 cut(s) 62, 488, 1078
Eam1105I GACNNNNNGTC 1 cut(s) 1508
EarI CTCTTC 3 cut(s) 62, 488, 1078
Ecl136II GAGCTC 2 cut(s) 55, 2182
Eco130I CCWWGG 3 cut(s) 873, 1157, 2217
Eco147I AGGCCT 1 cut(s) 1215
Eco24I GRGCYC 3 cut(s) 57, 1017, 2184
Eco32I GATATC 1 cut(s) 1396
Eco47I GGWCC 1 cut(s) 807
Eco53kI GAGCTC 2 cut(s) 55, 2182
Eco57I CTGAAG 2 cut(s) 83, 690
Eco88I CYCGRG 1 cut(s) 50
EcoICRI GAGCTC 2 cut(s) 55, 2182
EcoNI CCTNNNNNAGG 1 cut(s) 2109
EcoRII CCWGG 4 cut(s) 897, 1791, 1951, 2110
EcoRV GATATC 1 cut(s) 1396
EcoT14I CCWWGG 3 cut(s) 873, 1157, 2217
EcoT22I ATGCAT 2 cut(s) 1858, 2239
EcoT38I GRGCYC 3 cut(s) 57, 1017, 2184
ErhI CCWWGG 3 cut(s) 873, 1157, 2217
Esp3I CGTCTC 1 cut(s) 1201
FaeI CATG 6 cut(s) 872, 1094, 1261, 1282, 1328, 1528
FaqI GGGAC 2 cut(s) 1178, 2288
FatI CATG 6 cut(s) 868, 1090, 1257, 1278, 1324, 1524
FauI CCCGC 3 cut(s) 836, 1155, 2378
FauNDI CATATG 2 cut(s) 1483, 2239
FbaI TGATCA 2 cut(s) 976, 1102
FblI GTMKAC 2 cut(s) 1609, 1702
Fnu4HI GCNGC 6 cut(s) 22, 150, 297, 449, 576, 922
FokI GGATG 9 cut(s) 177, 486, 926, 1015, 1130, 1305, 1592, 1752, 2012
FriOI GRGCYC 3 cut(s) 57, 1017, 2184
Fsp4HI GCNGC 6 cut(s) 22, 150, 297, 449, 576, 922
FspBI CTAG 3 cut(s) 1550, 1671, 1817
GlaI GCGC 1 cut(s) 249
GluI GCNGC 6 cut(s) 22, 150, 297, 449, 576, 922
GsaI CCCAGC 1 cut(s) 218
GsuI CTGGAG 2 cut(s) 639, 1350
HaeIII GGCC 6 cut(s) 89, 325, 897, 1171, 1215, 1834
HapII CCGG 2 cut(s) 6, 810
HhaI GCGC 1 cut(s) 250
Hin1II CATG 6 cut(s) 872, 1094, 1261, 1282, 1328, 1528
Hin6I GCGC 1 cut(s) 248
HinP1I GCGC 1 cut(s) 248
HincII GTYRAC 2 cut(s) 1703, 2314
HindII GTYRAC 2 cut(s) 1703, 2314
HindIII AAGCTT 1 cut(s) 357
HinfI GANTC 7 cut(s) 1181, 1382, 1429, 1665, 1925, 2170, 2321
HpaII CCGG 2 cut(s) 6, 810
HphI GGTGA 5 cut(s) 109, 121, 482, 575, 2233
Hpy166II GTNNAC 5 cut(s) 1311, 1610, 1703, 1995, 2314
Hpy8I GTNNAC 5 cut(s) 1311, 1610, 1703, 1995, 2314
Hpy99I CGWCG 1 cut(s) 322
HpyAV CCTTC 5 cut(s) 31, 58, 583, 1220, 2115
HpyCH4III ACNGT 9 cut(s) 178, 199, 406, 933, 1522, 1891, 2029, 2157, 2356
HpyCH4IV ACGT 2 cut(s) 486, 1588
HpyF10VI GCNNNNNNNGC 5 cut(s) 155, 1168, 1258, 1643, 1853
HpyF3I CTNAG 7 cut(s) 702, 1241, 1970, 2046, 2178, 2331, 2363
HpySE526I ACGT 2 cut(s) 486, 1588
Hsp92II CATG 6 cut(s) 872, 1094, 1261, 1282, 1328, 1528
HspAI GCGC 1 cut(s) 248
Ksp22I TGATCA 2 cut(s) 976, 1102
Kzo9I GATC 7 cut(s) 511, 976, 1051, 1102, 1813, 2053, 2119
LguI GCTCTTC 1 cut(s) 62
LmnI GCTCC 5 cut(s) 445, 1012, 1726, 1747, 1954
Lsp1109I GCAGC 4 cut(s) 308, 460, 587, 908
LweI GCATC 7 cut(s) 155, 241, 508, 529, 1828, 1843, 1865
MaeI CTAG 3 cut(s) 1550, 1671, 1817
MaeII ACGT 2 cut(s) 486, 1588
MaeIII GTNAC 5 cut(s) 115, 1514, 1552, 1624, 2356
MalI GATC 7 cut(s) 513, 978, 1053, 1104, 1815, 2055, 2121
MboI GATC 7 cut(s) 511, 976, 1051, 1102, 1813, 2053, 2119
MfeI CAATTG 1 cut(s) 1173
MflI RGATCY 2 cut(s) 1051, 1813
MhlI GDGCHC 5 cut(s) 57, 1017, 1997, 2184, 2250
MlyI GAGTC 2 cut(s) 1190, 1423
MmeI TCCRAC 1 cut(s) 49
Mph1103I ATGCAT 2 cut(s) 1858, 2239
MroXI GAANNNNTTC 2 cut(s) 675, 2028
MseI TTAA 9 cut(s) 273, 408, 681, 800, 1457, 1541, 1697, 1920, 1989
MslI CAYNNNNRTG 5 cut(s) 1095, 1323, 1486, 1523, 1808
MspA1I CMGCKG 2 cut(s) 451, 1115
MspCI CTTAAG 1 cut(s) 799
MspI CCGG 2 cut(s) 6, 810
MspR9I CCNGG 5 cut(s) 6, 899, 1793, 1953, 2112
MunI CAATTG 1 cut(s) 1173
MvaI CCWGG 4 cut(s) 899, 1793, 1953, 2112
MwoI GCNNNNNNNGC 5 cut(s) 155, 1168, 1258, 1643, 1853
NciI CCSGG 1 cut(s) 6
NdeI CATATG 2 cut(s) 1483, 2239
NdeII GATC 7 cut(s) 511, 976, 1051, 1102, 1813, 2053, 2119
NlaIII CATG 6 cut(s) 872, 1094, 1261, 1282, 1328, 1528
NlaIV GGNNCC 4 cut(s) 324, 594, 1014, 1875
NmuCI GTSAC 3 cut(s) 115, 1552, 2356
NsiI ATGCAT 2 cut(s) 1858, 2239
NspI RCATGY 3 cut(s) 1261, 1282, 1328
PaeI GCATGC 1 cut(s) 1328
PaeR7I CTCGAG 1 cut(s) 50
PceI AGGCCT 1 cut(s) 1215
PciI ACATGT 1 cut(s) 1278
PciSI GCTCTTC 1 cut(s) 62
PcsI WCGNNNNNNNCGW 2 cut(s) 75, 203
PdmI GAANNNNTTC 2 cut(s) 675, 2028
PfeI GAWTC 5 cut(s) 1382, 1665, 1925, 2170, 2321
PkrI GCNGC 6 cut(s) 23, 151, 298, 450, 577, 923
PleI GAGTC 2 cut(s) 1189, 1423
PpsI GAGTC 2 cut(s) 1189, 1423
PscI ACATGT 1 cut(s) 1278
PshAI GACNNNNGTC 1 cut(s) 1587
PsiI TTATAA 2 cut(s) 720, 1275
Psp124BI GAGCTC 2 cut(s) 57, 2184
Psp6I CCWGG 4 cut(s) 897, 1791, 1951, 2110
PspFI CCCAGC 1 cut(s) 214
PspGI CCWGG 4 cut(s) 897, 1791, 1951, 2110
PspN4I GGNNCC 4 cut(s) 324, 594, 1014, 1875
PspPI GGNCC 2 cut(s) 323, 807
PspXI VCTCGAGB 1 cut(s) 50
PstNI CAGNNNCTG 1 cut(s) 956
PsuI RGATCY 2 cut(s) 1051, 1813
RsaI GTAC 4 cut(s) 1058, 1133, 1445, 1761
RsaNI GTAC 4 cut(s) 1057, 1132, 1444, 1760
RseI CAYNNNNRTG 5 cut(s) 1095, 1323, 1486, 1523, 1808
SacI GAGCTC 2 cut(s) 57, 2184
SalI GTCGAC 1 cut(s) 1701
SapI GCTCTTC 1 cut(s) 62
SaqAI TTAA 9 cut(s) 273, 408, 681, 800, 1457, 1541, 1697, 1920, 1989
SatI GCNGC 6 cut(s) 22, 150, 297, 449, 576, 922
Sau3AI GATC 7 cut(s) 511, 976, 1051, 1102, 1813, 2053, 2119
Sau96I GGNCC 2 cut(s) 323, 807
SchI GAGTC 2 cut(s) 1190, 1423
ScrFI CCNGG 5 cut(s) 6, 899, 1793, 1953, 2112
SduI GDGCHC 5 cut(s) 57, 1017, 1997, 2184, 2250
SfaNI GCATC 7 cut(s) 155, 241, 508, 529, 1828, 1843, 1865
Sfr274I CTCGAG 1 cut(s) 50
SinI GGWCC 1 cut(s) 807
SlaI CTCGAG 1 cut(s) 50
SmiMI CAYNNNNRTG 5 cut(s) 1095, 1323, 1486, 1523, 1808
SmlI CTYRAG 9 cut(s) 50, 788, 799, 1388, 1706, 1772, 1842, 2033, 2126
SmoI CTYRAG 9 cut(s) 50, 788, 799, 1388, 1706, 1772, 1842, 2033, 2126
SpeI ACTAGT 1 cut(s) 1549
SphI GCATGC 1 cut(s) 1328
SseBI AGGCCT 1 cut(s) 1215
SspI AATATT 1 cut(s) 1496
SspMI CTAG 3 cut(s) 1550, 1671, 1817
SstI GAGCTC 2 cut(s) 57, 2184
StuI AGGCCT 1 cut(s) 1215
StyD4I CCNGG 5 cut(s) 4, 897, 1791, 1951, 2110
StyI CCWWGG 3 cut(s) 873, 1157, 2217
TaaI ACNGT 9 cut(s) 178, 199, 406, 933, 1522, 1891, 2029, 2157, 2356
TaiI ACGT 2 cut(s) 489, 1591
TaqI TCGA 9 cut(s) 51, 172, 266, 692, 1221, 1546, 1702, 1915, 2184
TaqII GACCGA 1 cut(s) 2166
TatI WGTACW 2 cut(s) 1131, 1759
TauI GCSGC 2 cut(s) 24, 152
TfiI GAWTC 5 cut(s) 1382, 1665, 1925, 2170, 2321
Tru1I TTAA 9 cut(s) 273, 408, 681, 800, 1457, 1541, 1697, 1920, 1989
Tru9I TTAA 9 cut(s) 273, 408, 681, 800, 1457, 1541, 1697, 1920, 1989
TscAI CASTG 7 cut(s) 1042, 1074, 1376, 1525, 1605, 2096, 2359
TseFI GTSAC 3 cut(s) 115, 1552, 2356
TseI GCWGC 4 cut(s) 296, 448, 575, 921
Tsp45I GTSAC 3 cut(s) 115, 1552, 2356
TspDTI ATGAA 4 cut(s) 294, 716, 1163, 1262
TspGWI ACGGA 2 cut(s) 492, 2012
TspRI CASTG 7 cut(s) 1042, 1074, 1376, 1525, 1605, 2096, 2359
Vha464I CTTAAG 1 cut(s) 799
VneI GTGCAC 1 cut(s) 1993
VpaK11BI GGWCC 1 cut(s) 807
XagI CCTNNNNNAGG 1 cut(s) 2109
XapI RAATTY 3 cut(s) 331, 347, 1421
XbaI TCTAGA 1 cut(s) 1816
XceI RCATGY 3 cut(s) 1261, 1282, 1328
XcmI CCANNNNNNNNNTGG 1 cut(s) 1325
XhoI CTCGAG 1 cut(s) 50
XmiI GTMKAC 2 cut(s) 1609, 1702
XmnI GAANNNNTTC 2 cut(s) 675, 2028
XspI CTAG 3 cut(s) 1550, 1671, 1817
Zsp2I ATGCAT 2 cut(s) 1858, 2239
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.