Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0000937

Physical Location & Seq

Genomic Coordinates Reverse (-)

26288228 .. 26293173

Gene Structure 4946 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT1G69830.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2664 bp

ATGTCCACTGTTCCCATTGAGTCTCTTCTCCACCATTCTTATCTTCGTCACAACTCAAAAGTCAATCGCGGAAATAGAAGCTTCATACCTATCTCGTTGAATCTCCGTTCTCATTTCACTTCTAACAAACTACTACACTCAATTGGAAAAAGTGTCGGTGTTAGCTCGATGAACAAAAGTCCCGTCGCCATTCGTGCTACCTCATCGGATACTGCCGTCGTGGAAACCGCTCAATCGGATGATGTCATCTTCAAGGAAATTTTCCCTGTTCAGCGAATCGAAAAGGCAGAAGGAAAGATTTATGTTCGATTAAAGGAAGTGAAGGAGAAGAATTGGGAGCTGAGTGTTGGATGTAGTATACCTGGAAAATGGATTTTGCATTGGGGAGTTTCATATGTGGGTGATACTGGCAGTGAATGGGATCAACCTCCGGAAGATATGAGACCTCCTGGCTCAATTGCCATTAAGGACTATGCCATAGAGACACCTTTGAAGAAGTTATCTGAAGGAGATTCTTTTTTTGAAGTTGCTATTAATCTAAATCTGGAGAGTTCTGTAGCAGCTCTCAATTTTGTTTTGAAGGACGAAGAAACTGGGGCGTGGTATCAGCACAAAGGGAGAGACTTTAAGGTTCCTCTTGTAGACGATGTTCCTGATAATGGAAATTTGATCGGAGCTAAGAAAGGATTTGGTGCCCTTGGGCAGCTTTCAAACATCCCTCTCAAACAAGATAAGTCCAGTGCAGAGACTGATTCTATTGAAGAAAGAAAAGGTCTTCAAGAGTTTTACGAGGAGATGCCAATAAGTAAACGTGTTGCCGATGATAACTCAGTCAGTGTCACTGCAAGGAAATGCCCTGAAACATCTAAGAACATTGTATCAATTGAAACCGATTTACCGGGTGACGTTACTGTCCACTGGGGAGTTTGCAAAAACGGCACTAAGAAATGGGAAATTCCATCTGAACCTTACCCTGAAGAGACATCTTTGTTTAAGAACAAGGCATTACGCACTCGTTTACAGCGAAAAGACGATGGAAATGGATCGTTTGGATTATTCTCTCTGGATGGAAAGCTTGAAGGGTTATGCTTTGTTCTTAAGTTAAATGAAAATACTTGGCTAAATTATAGGGGGGAAGACTTCTATGTCCCTTTCCTTACTTCAAGTAGCTCGCCCGTTGAAACTGAAGCTGCCCAAGTGAGTAAACCCAAACGAAAAACAGATAAAGAAGTGTCTGCTAGTGGATTTACTAAAGAAATCATCACGGAGATAAGGAACTTGGCAATTGACATTTCCTCTCATAAGAATCAGAAGACAAACGTCAAAGAAGTGCAGGAAAACATTCTACAAGAAATTGAGAAACTGGCTGCGGAGGCATATAGCATATTTAGAAGCACAACTCCAGCTTTTTCCGAGGAAGGTGTTTTAGAAGCAGAAGCTGACAAGCCTGACATTAAAATCTCCTCAGGAACCGGCTCGGGATTTGAGATATTATGCCAAGGTTTCAACTGGGAGTCCAATAAATCTGGGAGATGGTACTTGGAACTTCAAGAAAAAGCCGATGAGTTAGCTTCACTTGGATTCACTGTTCTGTGGTTACCTCCACCGACAGAATCTGTGTCACCTGAAGGGTACATGCCTAAAGACCTGTATAACTTGAATTCCAGATATGGAACAATTGATGAGCTAAAAGATACAGTGAAGAAATTTCACAAGGTCGGGATCAAAGTTTTAGGCGATGCTGTTCTGAATCACCGCTGTGCACACTTCAAAAATCAAAATGGTGTATGGAATCTATTTGGAGGACGTCTAAACTGGGACGATAGGGCAGTAGTTGCAGATGACCCTCATTTCCAGGGTAGAGGAAACAAGAGCAGTGGAGATAATTTCCATGCTGCTCCAAACATAGATCACTCGCAAGACTTTGTTAGGAAGGATATCAAGGAATGGCTATGCTGGATGATGGAAGAAGTTGGGTATGATGGATGGAGGCTTGACTTTGTAAGAGGGTTCTGGGGAGGTTATGTGAAAGACTATATGGATGCTAGCAAACCGTACTTTGCGGTTGGTGAATATTGGGATTCGTTAAGTTACACGTACGGAGAAATGGACTACAATCAAGACGCACATCGTCAAAGAATAGTTGACTGGATTAATGCAACTAGTGGAGCTGCTGGTGCATTTGATGTCACTACCAAAGGAATTCTTCATACGGCGCTTCAAAAATGTGAATATTGGAGACTCTCAGACCCAAAAGGGAAGCCTCCAGGTGTAGTTGGTTGGTGGCCTTCTCGTGCTGTAACATTCATCGAGAATCATGACACTGGCTCTACACAGGGTCATTGGAGATTTCCGGAGGGGAAGGAAATGCAAGGATATGCTTACATCCTAACTCATCCAGGAACACCAGCGGTCTTCTTCGACCATATCTTCTCGGATTATCATTCCGAGATTGCTGCACTTCTCTCTCTCAGGAACAGACAGAAACTCCACTGTCGGAGTGAGGTGAATATAGACAAGAGTGAGAGAGATGTGTATGCGGCTATAATAGATGAAAAGGTTGCAATGAAGATCGGACCAGGGCATTATGAACCACCAAACGGATCGCAAAACTGGTCTGTAGCCGTTGAAGGCAGAGACTACAAGGTGTGGGAAACATCTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000272 GO:0003674 GO:0003824 GO:0004553 GO:0004556 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0005976 GO:0005982 GO:0005983 GO:0005984 GO:0005985 GO:0005987 GO:0006073 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009251 GO:0009311 GO:0009313 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009987 GO:0016052 GO:0016160 GO:0016787 GO:0016798 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044042 GO:0044237 GO:0044238 GO:0044247 GO:0044248 GO:0044260 GO:0044262 GO:0044264 GO:0044275 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046352 GO:0071704 GO:1901575

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Alpha-amyl_C2 Alpha-amylase

Protein Analysis

887

Amino Acids

99.84

Weight (kDa)

5.73

Isoelectric Point (pI)

39.53

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ig_N_CWD1	PF23166	95 - 213	8.5e-28	Alpha-glucan water dikinase 1 Ig-like N-terminal domain
Ig_N_CWD1	PF23166	268 - 385	3e-34	Alpha-glucan water dikinase 1 Ig-like N-terminal domain
Alpha-amylase	PF00128	517 - 590	2.5e-12	Alpha amylase, catalytic domain
Alpha-amyl_C2	PF07821	827 - 885	2e-21	Alpha-amylase C-terminal beta-sheet domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0000937)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G69830
fragaria_vesca	FvH4_4g31910 FvH4_4g31910
malus_domestica	MD13G1078600.v1.1 MD16G1078200.v1.1
prunus_persica	Prupe.1G268300_v2.0.a1 Prupe.1G268300_v2.0.a1
pyrus_communis	pycom13g06820 pycom16g06770
rosa_chinensis	RchiOBHm_Chr1g0315221 RchiOBHm_Chr1g0339091 RchiOBHm_Chr4g0440561 RchiOBHm_Chr5g0021421 RchiOBHm_Chr6g0272311
rosa_laevigata	RLG00000006168 RLG00000015039 RLG00000019642 RLG00000030692
rosa_multiflora	Rmu_co8071990.1_g000001 Rmu_sc0000829.1_g000011 Rmu_sc0002387.1_g000011 Rmu_sc0011312.1_g000002
rosa_roxburghii	Rroxscaffold_4G00298760 Rroxscaffold_5G00381380 Rroxscaffold_6G00426500 Rroxscaffold_7G00169140 Rroxscaffold_7G00214180
rosa_rugosa	Rorug01G0004200 Rorug04G0326700 Rorug05G0356300 Rorug05G0477400 Rorug05G0527600 Rorug07G0284000
rosa_samantha	Rh1AG270700 Rh1AG270800 Rh1AG346400 Rh1BG260100 Rh1CG254500 Rh2AG416800 Rh2CG403000 Rh3DG074600 Rh3DG292400 Rh4AG190200 Rh4AG246600 Rh4AG378400 Rh4BG035500 Rh4BG391100 Rh4CG405800 Rh4DG085900 Rh4DG385000 Rh5AG156300 Rh5AG268600 Rh5AG480700 Rh5BG155700 Rh5CG169500 Rh6AG024000 Rh6BG089200 Rh6DG025600 Rh7CG251800
rosa_wichuraiana	Rw1G024120 Rw1G030800 Rw4G032400 Rw4G032500 Rw7G034860

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	911, 1145
AatII	GACGTC	1 cut(s)	1810
AccB1I	GGYRCC	1 cut(s)	692
AccBSI	CCGCTC	1 cut(s)	230
AccI	GTMKAC	2 cut(s)	358, 642
AccII	CGCG	1 cut(s)	69
AccIII	TCCGGA	2 cut(s)	430, 2353
AciI	CCGC	7 cut(s)	69, 228, 1370, 1756, 2063, 2411, 2540
AclWI	GGATC	4 cut(s)	429, 1051, 1730, 2611
AcsI	RAATTY	6 cut(s)	258, 664, 954, 1660, 1706, 2202
AcuI	CTGAAG	4 cut(s)	525, 996, 1206, 1647
AcyI	GRCGYC	1 cut(s)	1807
AfaI	GTAC	4 cut(s)	1538, 1634, 2057, 2099
AfiI	CCNNNNNNNGG	2 cut(s)	659, 2600
AflII	CTTAAG	1 cut(s)	1097
AflIII	ACRYGT	2 cut(s)	811, 2094
AhlI	ACTAGT	1 cut(s)	2162
AjnI	CCWGG	6 cut(s)	361, 448, 1854, 2266, 2398, 2578
AleI	CACNNNNGTG	1 cut(s)	1758
AloI	GAACNNNNNNTCC	2 cut(s)	1572, 1604
Alw21I	GWGCWC	1 cut(s)	1765
Alw26I	GTCTC	8 cut(s)	27, 436, 476, 615, 740, 974, 2233, 2631
Alw44I	GTGCAC	1 cut(s)	1761
AlwI	GGATC	4 cut(s)	429, 1051, 1730, 2611
AlwNI	CAGNNNCTG	3 cut(s)	749, 1439, 1616
Ama87I	CYCGRG	1 cut(s)	1477
Aor13HI	TCCGGA	2 cut(s)	430, 2353
AoxI	GGCC	1 cut(s)	2285
ApaLI	GTGCAC	1 cut(s)	1761
ApeKI	GCWGC	7 cut(s)	560, 703, 1190, 1367, 1895, 2171, 2456
ApoI	RAATTY	6 cut(s)	258, 664, 954, 1660, 1706, 2202
ArsI	GACNNNNNNTTYG	2 cut(s)	168, 200
AseI	ATTAAT	2 cut(s)	534, 2154
Asp700I	GAANNNNTTC	1 cut(s)	1341
AspLEI	GCGC	1 cut(s)	2218
AspS9I	GGNCC	1 cut(s)	2576
AsuC2I	CCSGG	1 cut(s)	900
AsuHPI	GGTGA	6 cut(s)	413, 914, 1614, 1745, 2081, 2518
AsuNHI	GCTAGC	1 cut(s)	2045
AvaI	CYCGRG	1 cut(s)	1477
AvaII	GGWCC	1 cut(s)	2576
AxyI	CCTNAGG	1 cut(s)	1465
BaeGI	GKGCMC	2 cut(s)	697, 1765
BaeI	ACNNNNGTAYC	2 cut(s)	201, 234
BanI	GGYRCC	1 cut(s)	692
BauI	CACGAG	1 cut(s)	2292
BbsI	GAAGAC	4 cut(s)	767, 1143, 1319, 2407
Bbv12I	GWGCWC	1 cut(s)	1765
BbvI	GCAGC	7 cut(s)	572, 715, 1177, 1354, 1882, 2158, 2443
BccI	CCATC	7 cut(s)	967, 1028, 1061, 1527, 1957, 1976, 1980
BceAI	ACGGC	4 cut(s)	200, 952, 2229, 2609
BcgI	CGANNNNNNTGC	2 cut(s)	2438, 2472
BciT130I	CCWGG	6 cut(s)	363, 450, 1856, 2268, 2400, 2580
BciVI	GTATCC	1 cut(s)	202
BcnI	CCSGG	1 cut(s)	900
BcoDI	GTCTC	8 cut(s)	27, 436, 476, 615, 740, 974, 2233, 2631
BcuI	ACTAGT	1 cut(s)	2162
BfaI	CTAG	3 cut(s)	1239, 2046, 2163
BfmI	CTRYAG	2 cut(s)	555, 2619
BfoI	RGCGCY	1 cut(s)	2219
BfrI	CTTAAG	1 cut(s)	1097
BfuI	GTATCC	1 cut(s)	202
BisI	GCNGC	8 cut(s)	561, 704, 1191, 1368, 1896, 2172, 2457, 2541
BlsI	GCNGC	8 cut(s)	562, 705, 1192, 1369, 1897, 2173, 2458, 2542
Bme1390I	CCNGG	7 cut(s)	363, 450, 900, 1856, 2268, 2400, 2580
Bme18I	GGWCC	1 cut(s)	2576
BmeT110I	CYCGRG	1 cut(s)	1477
BmgT120I	GGNCC	1 cut(s)	2576
BmiI	GGNNCC	3 cut(s)	633, 694, 1471
BmrFI	CCNGG	7 cut(s)	363, 450, 900, 1856, 2268, 2400, 2580
BmrI	ACTGGG	4 cut(s)	603, 928, 1519, 1825
BmsI	GCATC	3 cut(s)	786, 1729, 2032
BmtI	GCTAGC	1 cut(s)	2049
BmuI	ACTGGG	4 cut(s)	603, 928, 1519, 1825
BoxI	GACNNNNGTC	1 cut(s)	1319
BpiI	GAAGAC	4 cut(s)	767, 1143, 1319, 2407
BpmI	CTGGAG	3 cut(s)	566, 1386, 2250
BpuMI	CCSGG	1 cut(s)	900
BsaAI	YACGTR	1 cut(s)	2097
BsaHI	GRCGYC	1 cut(s)	1807
BsaI	GGTCTC	1 cut(s)	436
BsaJI	CCNNGG	5 cut(s)	697, 1413, 1498, 1855, 2579
BsaWI	WCCGGW	2 cut(s)	430, 2353
BsaXI	ACNNNNNCTCC	4 cut(s)	2010, 2040, 2472, 2502
Bsc4I	CCNNNNNNNGG	2 cut(s)	659, 2600
Bse118I	RCCGGY	1 cut(s)	1472
Bse21I	CCTNAGG	1 cut(s)	1465
Bse3DI	GCAATG	1 cut(s)	2571
BseAI	TCCGGA	2 cut(s)	430, 2353
BseBI	CCWGG	6 cut(s)	363, 450, 1856, 2268, 2400, 2580
BseDI	CCNNGG	5 cut(s)	697, 1413, 1498, 1855, 2579
BseGI	GGATG	9 cut(s)	244, 356, 714, 1072, 1965, 1991, 2047, 2385, 2395
BseLI	CCNNNNNNNGG	2 cut(s)	659, 2600
BseMI	GCAATG	1 cut(s)	2571
BseMII	CTCAG	5 cut(s)	332, 843, 1479, 2259, 2485
BseRI	GAGGAG	2 cut(s)	806, 1453
BseSI	GKGCMC	2 cut(s)	697, 1765
BseXI	GCAGC	7 cut(s)	572, 715, 1177, 1354, 1882, 2158, 2443
BsgI	GTGCAG	3 cut(s)	762, 1352, 2442
Bsh1236I	CGCG	1 cut(s)	69
BshFI	GGCC	1 cut(s)	2287
BshNI	GGYRCC	1 cut(s)	692
BsiHKAI	GWGCWC	1 cut(s)	1765
BsiHKCI	CYCGRG	1 cut(s)	1477
BsiSI	CCGG	4 cut(s)	431, 899, 1473, 2354
BsiWI	CGTACG	1 cut(s)	2097
BslFI	GGGAC	3 cut(s)	165, 1133, 1832
BslI	CCNNNNNNNGG	2 cut(s)	659, 2600
BsmAI	GTCTC	8 cut(s)	27, 436, 476, 615, 740, 974, 2233, 2631
BsmFI	GGGAC	3 cut(s)	165, 1133, 1832
BsnI	GGCC	1 cut(s)	2287
Bso31I	GGTCTC	1 cut(s)	436
BsoBI	CYCGRG	1 cut(s)	1477
Bsp1286I	GDGCHC	2 cut(s)	697, 1765
Bsp13I	TCCGGA	2 cut(s)	430, 2353
Bsp143I	GATC	7 cut(s)	421, 669, 1043, 1722, 1909, 2571, 2603
BspACI	CCGC	7 cut(s)	69, 228, 1370, 1756, 2063, 2411, 2540
BspANI	GGCC	1 cut(s)	2287
BspCNI	CTCAG	5 cut(s)	333, 842, 1478, 2258, 2484
BspEI	TCCGGA	2 cut(s)	430, 2353
BspFNI	CGCG	1 cut(s)	69
BspHI	TCATGA	1 cut(s)	2317
BspLI	GGNNCC	3 cut(s)	633, 694, 1471
BspOI	GCTAGC	1 cut(s)	2049
BspPI	GGATC	4 cut(s)	429, 1051, 1730, 2611
BspT107I	GGYRCC	1 cut(s)	692
BspTI	CTTAAG	1 cut(s)	1097
BspTNI	GGTCTC	1 cut(s)	436
BsrBI	CCGCTC	1 cut(s)	230
BsrDI	GCAATG	1 cut(s)	2571
BsrFI	RCCGGY	1 cut(s)	1472
BssAI	RCCGGY	1 cut(s)	1472
BssECI	CCNNGG	5 cut(s)	697, 1413, 1498, 1855, 2579
BssMI	GATC	7 cut(s)	421, 669, 1043, 1722, 1909, 2571, 2603
BssNAI	GTATAC	1 cut(s)	359
BssNI	GRCGYC	1 cut(s)	1807
BssSI	CACGAG	1 cut(s)	2292
BssT1I	CCWWGG	2 cut(s)	697, 1498
Bst1107I	GTATAC	1 cut(s)	359
Bst2BI	CACGAG	1 cut(s)	2292
Bst2UI	CCWGG	6 cut(s)	363, 450, 1856, 2268, 2400, 2580
Bst4CI	ACNGT	6 cut(s)	10, 913, 1588, 1699, 2055, 2495
Bst6I	CTCTTC	2 cut(s)	30, 972
BstACI	GRCGYC	1 cut(s)	1807
BstAFI	CTTAAG	1 cut(s)	1097
BstAPI	GCANNNNNTGC	1 cut(s)	1835
BstBAI	YACGTR	1 cut(s)	2097
BstC8I	GCNNGC	2 cut(s)	1172, 2047
BstDEI	CTNAG	8 cut(s)	341, 678, 829, 867, 942, 1465, 2245, 2471
BstEII	GGTNACC	1 cut(s)	1596
BstF5I	GGATG	9 cut(s)	244, 356, 714, 1072, 1965, 1991, 2047, 2385, 2395
BstFNI	CGCG	1 cut(s)	69
BstH2I	RGCGCY	1 cut(s)	2219
BstHHI	GCGC	1 cut(s)	2218
BstKTI	GATC	7 cut(s)	424, 672, 1046, 1725, 1912, 2574, 2606
BstMAI	GTCTC	8 cut(s)	27, 436, 476, 615, 740, 974, 2233, 2631
BstMBI	GATC	7 cut(s)	421, 669, 1043, 1722, 1909, 2571, 2603
BstMWI	GCNNNNNNNGC	5 cut(s)	194, 936, 1373, 1835, 2177
BstNI	CCWGG	6 cut(s)	363, 450, 1856, 2268, 2400, 2580
BstNSI	RCATGY	1 cut(s)	1639
BstPAI	GACNNNNGTC	1 cut(s)	1319
BstPI	GGTNACC	1 cut(s)	1596
BstSCI	CCNGG	7 cut(s)	361, 448, 898, 1854, 2266, 2398, 2578
BstSFI	CTRYAG	2 cut(s)	555, 2619
BstSLI	GKGCMC	2 cut(s)	697, 1765
BstUI	CGCG	1 cut(s)	69
BstV1I	GCAGC	7 cut(s)	572, 715, 1177, 1354, 1882, 2158, 2443
BstV2I	GAAGAC	4 cut(s)	767, 1143, 1319, 2407
BstZ17I	GTATAC	1 cut(s)	359
Bsu36I	CCTNAGG	1 cut(s)	1465
BsuI	GTATCC	1 cut(s)	202
BsuRI	GGCC	1 cut(s)	2287
BtgZI	GCGATG	1 cut(s)	1752
BtsCI	GGATG	9 cut(s)	244, 356, 714, 1072, 1965, 1991, 2047, 2385, 2395
BtsI	GCAGTG	3 cut(s)	418, 840, 1882
Cac8I	GCNNGC	2 cut(s)	1172, 2047
CaiI	CAGNNNCTG	3 cut(s)	749, 1439, 1616
CciI	TCATGA	1 cut(s)	2317
CfoI	GCGC	1 cut(s)	2218
Cfr10I	RCCGGY	1 cut(s)	1472
Cfr13I	GGNCC	1 cut(s)	2576
CseI	GACGC	1 cut(s)	2132
Csp6I	GTAC	4 cut(s)	1537, 1633, 2056, 2098
CspCI	CAANNNNNGTGG	2 cut(s)	1858, 1893
CviAII	CATG	3 cut(s)	1636, 1892, 2318
CviQI	GTAC	4 cut(s)	1537, 1633, 2056, 2098
DdeI	CTNAG	8 cut(s)	341, 678, 829, 867, 942, 1465, 2245, 2471
DpnI	GATC	7 cut(s)	423, 671, 1045, 1724, 1911, 2573, 2605
DpnII	GATC	7 cut(s)	421, 669, 1043, 1722, 1909, 2571, 2603
DrdI	GACNNNNNNGTC	2 cut(s)	911, 1145
DseDI	GACNNNNNNGTC	2 cut(s)	911, 1145
Eam1104I	CTCTTC	2 cut(s)	30, 972
EarI	CTCTTC	2 cut(s)	30, 972
Eco130I	CCWWGG	2 cut(s)	697, 1498
Eco31I	GGTCTC	1 cut(s)	436
Eco32I	GATATC	1 cut(s)	1939
Eco47I	GGWCC	1 cut(s)	2576
Eco57I	CTGAAG	4 cut(s)	525, 996, 1206, 1647
Eco81I	CCTNAGG	1 cut(s)	1465
Eco88I	CYCGRG	1 cut(s)	1477
Eco91I	GGTNACC	1 cut(s)	1596
EcoO65I	GGTNACC	1 cut(s)	1596
EcoRI	GAATTC	2 cut(s)	1660, 2202
EcoRII	CCWGG	6 cut(s)	361, 448, 1854, 2266, 2398, 2578
EcoRV	GATATC	1 cut(s)	1939
EcoT14I	CCWWGG	2 cut(s)	697, 1498
ErhI	CCWWGG	2 cut(s)	697, 1498
FaeI	CATG	3 cut(s)	1639, 1895, 2321
FalI	AAGNNNNNCTT	2 cut(s)	2190, 2222
FaqI	GGGAC	3 cut(s)	165, 1133, 1832
FatI	CATG	3 cut(s)	1635, 1891, 2317
FauNDI	CATATG	1 cut(s)	394
FblI	GTMKAC	2 cut(s)	358, 642
Fnu4HI	GCNGC	8 cut(s)	561, 704, 1191, 1368, 1896, 2172, 2457, 2541
FokI	GGATG	9 cut(s)	251, 363, 701, 1079, 1972, 1998, 2054, 2372, 2382
Fsp4HI	GCNGC	8 cut(s)	561, 704, 1191, 1368, 1896, 2172, 2457, 2541
FspBI	CTAG	3 cut(s)	1239, 2046, 2163
GlaI	GCGC	1 cut(s)	2217
GluI	GCNGC	8 cut(s)	561, 704, 1191, 1368, 1896, 2172, 2457, 2541
GsuI	CTGGAG	3 cut(s)	566, 1386, 2250
HaeII	RGCGCY	1 cut(s)	2219
HaeIII	GGCC	1 cut(s)	2287
HapII	CCGG	4 cut(s)	431, 899, 1473, 2354
HgaI	GACGC	1 cut(s)	2132
HhaI	GCGC	1 cut(s)	2218
Hin1I	GRCGYC	1 cut(s)	1807
Hin1II	CATG	3 cut(s)	1639, 1895, 2321
Hin6I	GCGC	1 cut(s)	2216
HinP1I	GCGC	1 cut(s)	2216
HincII	GTYRAC	1 cut(s)	2146
HindII	GTYRAC	1 cut(s)	2146
HindIII	AAGCTT	2 cut(s)	79, 1073
HpaII	CCGG	4 cut(s)	431, 899, 1473, 2354
HphI	GGTGA	6 cut(s)	413, 914, 1614, 1745, 2081, 2518
Hpy166II	GTNNAC	9 cut(s)	6, 359, 643, 809, 916, 1019, 1205, 1763, 2146
Hpy8I	GTNNAC	9 cut(s)	6, 359, 643, 809, 916, 1019, 1205, 1763, 2146
Hpy99I	CGWCG	2 cut(s)	188, 221
HpyCH4III	ACNGT	6 cut(s)	10, 913, 1588, 1699, 2055, 2495
HpyCH4IV	ACGT	5 cut(s)	811, 906, 1320, 1807, 2096
HpyF10VI	GCNNNNNNNGC	5 cut(s)	194, 936, 1373, 1835, 2177
HpyF3I	CTNAG	8 cut(s)	341, 678, 829, 867, 942, 1465, 2245, 2471
HpySE526I	ACGT	5 cut(s)	811, 906, 1320, 1807, 2096
Hsp92I	GRCGYC	1 cut(s)	1807
Hsp92II	CATG	3 cut(s)	1639, 1895, 2321
HspAI	GCGC	1 cut(s)	2216
Kpn2I	TCCGGA	2 cut(s)	430, 2353
Kzo9I	GATC	7 cut(s)	421, 669, 1043, 1722, 1909, 2571, 2603
LmnI	GCTCC	4 cut(s)	337, 674, 1903, 2168
Lsp1109I	GCAGC	7 cut(s)	572, 715, 1177, 1354, 1882, 2158, 2443
LweI	GCATC	3 cut(s)	786, 1729, 2032
MaeI	CTAG	3 cut(s)	1239, 2046, 2163
MaeII	ACGT	5 cut(s)	811, 906, 1320, 1807, 2096
MaeIII	GTNAC	9 cut(s)	47, 838, 902, 907, 1596, 1620, 2090, 2188, 2299
MalI	GATC	7 cut(s)	423, 671, 1045, 1724, 1911, 2573, 2605
MbiI	CCGCTC	1 cut(s)	230
MboI	GATC	7 cut(s)	421, 669, 1043, 1722, 1909, 2571, 2603
MfeI	CAATTG	5 cut(s)	141, 456, 882, 1284, 1677
MhlI	GDGCHC	2 cut(s)	697, 1765
MlyI	GAGTC	3 cut(s)	29, 1523, 2235
MmeI	TCCRAC	2 cut(s)	328, 2477
MroI	TCCGGA	2 cut(s)	430, 2353
MroXI	GAANNNNTTC	1 cut(s)	1341
MslI	CAYNNNNRTG	1 cut(s)	1758
MspA1I	CMGCKG	2 cut(s)	1758, 2411
MspCI	CTTAAG	1 cut(s)	1097
MspI	CCGG	4 cut(s)	431, 899, 1473, 2354
MspR9I	CCNGG	7 cut(s)	363, 450, 900, 1856, 2268, 2400, 2580
MunI	CAATTG	5 cut(s)	141, 456, 882, 1284, 1677
MvaI	CCWGG	6 cut(s)	363, 450, 1856, 2268, 2400, 2580
MvnI	CGCG	1 cut(s)	69
MwoI	GCNNNNNNNGC	5 cut(s)	194, 936, 1373, 1835, 2177
NciI	CCSGG	1 cut(s)	900
NdeI	CATATG	1 cut(s)	394
NdeII	GATC	7 cut(s)	421, 669, 1043, 1722, 1909, 2571, 2603
NheI	GCTAGC	1 cut(s)	2045
NlaIII	CATG	3 cut(s)	1639, 1895, 2321
NlaIV	GGNNCC	3 cut(s)	633, 694, 1471
NmuCI	GTSAC	5 cut(s)	47, 838, 902, 1620, 2188
NspI	RCATGY	1 cut(s)	1639
OliI	CACNNNNGTG	1 cut(s)	1758
PagI	TCATGA	1 cut(s)	2317
PcsI	WCGNNNNNNNCGW	1 cut(s)	1021
PdmI	GAANNNNTTC	1 cut(s)	1341
Pfl23II	CGTACG	1 cut(s)	2097
PfoI	TCCNGGA	1 cut(s)	2398
PkrI	GCNGC	8 cut(s)	562, 705, 1192, 1369, 1897, 2173, 2458, 2542
PleI	GAGTC	3 cut(s)	28, 1522, 2235
PpsI	GAGTC	3 cut(s)	28, 1522, 2235
Ppu21I	YACGTR	1 cut(s)	2097
PshAI	GACNNNNGTC	1 cut(s)	1319
PshBI	ATTAAT	2 cut(s)	534, 2154
Psp6I	CCWGG	6 cut(s)	361, 448, 1854, 2266, 2398, 2578
PspEI	GGTNACC	1 cut(s)	1596
PspGI	CCWGG	6 cut(s)	361, 448, 1854, 2266, 2398, 2578
PspLI	CGTACG	1 cut(s)	2097
PspN4I	GGNNCC	3 cut(s)	633, 694, 1471
PspPI	GGNCC	1 cut(s)	2576
PsrI	GAACNNNNNNTAC	2 cut(s)	633, 665
PstNI	CAGNNNCTG	3 cut(s)	749, 1439, 1616
RsaI	GTAC	4 cut(s)	1538, 1634, 2057, 2099
RsaNI	GTAC	4 cut(s)	1537, 1633, 2056, 2098
RseI	CAYNNNNRTG	1 cut(s)	1758
SatI	GCNGC	8 cut(s)	561, 704, 1191, 1368, 1896, 2172, 2457, 2541
Sau3AI	GATC	7 cut(s)	421, 669, 1043, 1722, 1909, 2571, 2603
Sau96I	GGNCC	1 cut(s)	2576
SchI	GAGTC	3 cut(s)	29, 1523, 2235
ScrFI	CCNGG	7 cut(s)	363, 450, 900, 1856, 2268, 2400, 2580
SduI	GDGCHC	2 cut(s)	697, 1765
SfaNI	GCATC	3 cut(s)	786, 1729, 2032
SfcI	CTRYAG	2 cut(s)	555, 2619
SinI	GGWCC	1 cut(s)	2576
SmiMI	CAYNNNNRTG	1 cut(s)	1758
SmlI	CTYRAG	1 cut(s)	1097
SmoI	CTYRAG	1 cut(s)	1097
SpeI	ACTAGT	1 cut(s)	2162
SsiI	CCGC	7 cut(s)	69, 228, 1370, 1756, 2063, 2411, 2540
SspI	AATATT	2 cut(s)	2075, 2234
SspMI	CTAG	3 cut(s)	1239, 2046, 2163
StyD4I	CCNGG	7 cut(s)	361, 448, 898, 1854, 2266, 2398, 2578
StyI	CCWWGG	2 cut(s)	697, 1498
TaaI	ACNGT	6 cut(s)	10, 913, 1588, 1699, 2055, 2495
TaiI	ACGT	5 cut(s)	814, 909, 1323, 1810, 2099
TaqI	TCGA	5 cut(s)	167, 279, 307, 2310, 2421
TauI	GCSGC	1 cut(s)	2543
TseFI	GTSAC	5 cut(s)	47, 838, 902, 1620, 2188
TseI	GCWGC	7 cut(s)	560, 703, 1190, 1367, 1895, 2171, 2456
Tsp45I	GTSAC	5 cut(s)	47, 838, 902, 1620, 2188
TspDTI	ATGAA	9 cut(s)	73, 185, 381, 1122, 2198, 2296, 2568, 2582, 2604
TspGWI	ACGGA	4 cut(s)	95, 1280, 2115, 2616
Vha464I	CTTAAG	1 cut(s)	1097
VneI	GTGCAC	1 cut(s)	1761
VpaK11BI	GGWCC	1 cut(s)	2576
VspI	ATTAAT	2 cut(s)	534, 2154
XapI	RAATTY	6 cut(s)	258, 664, 954, 1660, 1706, 2202
XceI	RCATGY	1 cut(s)	1639
XmiI	GTMKAC	2 cut(s)	358, 642
XmnI	GAANNNNTTC	1 cut(s)	1341
XspI	CTAG	3 cut(s)	1239, 2046, 2163
ZraI	GACGTC	1 cut(s)	1808

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.