Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0000937

Physical Location & Seq

Genomic Coordinates Reverse (-)

5484910 .. 5491869

Gene Structure 6960 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1078200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2805 bp

ATGTTCTCAGTCTCCCGAGTGGCCGTTGCTCATACCATGTCGACGGTTAGGATAGAGCCCCTCCTCCAGGACCTCCACCACTACGGTCGACAAAAACCCAGCCACCGCCGTCCGCAGTCGAATCATCCATTAAAGCTCAGCTCTTCTTTCACTGCTTTTCCTAAGAAGCTAGTAGTCTCCAACAGCCGCAGCTTCTGTTACTTCCAGCCTCCCACTCCCAGGAGGGGTCCCACTCTCGGAGTCAGAGCTGCCTCCACCGATACAACCACTGTCGAGACCTCCGAATCCACCGATCCCATCTACAAGAAGACCTTCCCTCTCAAGCGGACGGAAGTGGTGGAGGGAAAGATCTTTGTGAAATTAGATCATGGGAAGAATGAAAAGAAGTGGGTGCTGACTGTGGGTTGTAATCTTCCTGGAAAATGGGTTCTTCATTGGGGAGTTTCTTTTGTGGACGATGTTAGTTGCGAATGGGAACAGCCTCCTAGTGAAATGAGACCAGCTGGTTCAGTTCCCATCAAGGACTATGCAATAGAGACACCCTTGAAGGAATCGTTGTCGTCTGTGGGAGGCGATACATCTTATGAAGTGAAGATTGATGTTAAACCCAACAGCGCAATTGCAGCGATAAATTTTGTTCTCAAGGATGAAGAAACTGGTGCTTGGTATCAGCACAGAGGGAGTGACTTTAGAGTGCCTCTTGTCGCCTACCCACAAGACGATGACAATGTAGTTGGAGCGACAAAGGGCTTTGGCATGTGGCCAGGAGTTTTGGGAAAACTATCCAACGTGTTCGTCAAAGCAGAATCATCAAATTCCAAAGATCAAGACAGCAGCGGCAGTGAATCCAGACACCCTCAACAGAAAACTACGTGTGTAGAAGGATTCTATGAAGAACTGCCAATTGCAAAAGAAATTTCTGTAAACAATTCAGTAACTGTTTCCGTTAGCAAGTGCCCTGAGACAGCTAAGAATCTACTATACTTGGAAACAGATTTACCTGATAATGCTGTTGTTCACTGGGGAGTTTGCAGAGATGATACTAAAACATGGGAAATTCCAGCTGCCCCGCATCCACCAGAAACAGTAGTATTCAAGGACAAGGCTTTGCGGACTCGATTACAGCAAAAAGAGGGTGGAAATGGATGCTGGGGACTGTTTACTTTGGAAGAAGGACTCGCAGGATTTCTTTTTCTTTTGAAACTAAATGAAAGTACGTGGTTGAGATGTGTGGGAAATGACTTCTACATCCCCCTTTCAAGCTCAAAAAACGCAAATGTTGTACAATCAGAGATTCAATCTAAAGATGCTCAGGTACCTGACGGAAGTACAGAAGCAGTAGAAGAAAGTACTGCATATGCCGATGGATTAATCAATGAAATGAGGAACTTGGTGAGTGACGTTTTCTCTGATAAAAGTCCAAGGACAAGATCCAAAAAAGCACAAGAAGCCATTCTTCAAGAAATAGAAAAATTGGCTGCTGAAGCGTATAGTATCTTCAGAACTACCGTTCCAACTTTACCTGAGGAAACCATTGCAGAAACTGAAGAAGTGAAGGTCGCCCCTGCCAAAATATGTTCAGGGACAGGAACAGGTTTCGAAATATTGTGCCAAGGTTTTAACTGGGAATCTAGTAAATCTGGAAGATGGTACATGGAACTCAAAAGTAAAGCTGCACTGTTATCTTCACTAGGTTTCACTGTGATTTGGTTTCCACCTCCCACAGAGTCTGTTTCACCTGAAGGGTACATGCCCAGGGATTTATATAATCTGAACTCCAGATACGGAAATATGGACGAACTGAAGGAGACCGTGAAGGTATTCCATGACGTTGGTATAAAAGTTCTTGGAGATGCTGTTCTGAATCACCGTTGTGCACATTATCGGAATCAAAATGGTGTTTGGAATATATTTGGTGGTCGTTTAAATTGGGATGATCGTGCAGTTGTTGCAGATGATCCACATTTTCAGGGAAGGGGCAACAAAAGTAGTGGAGATAGTTTTCATGCAGCCCCAAACATTGATCATTCACAAGATTTTGTGAGGAAGGATATCAGAGAATGGTTATGCTGGCTAAGGAACGATATTGGGTACGATGGATGGAGGCTGGATTTTGTTAGAGGATTTTGGGGTGGCTATGTCAGGGACTACGTGGATGCTAGTGAGCCCTACTTTGCTGTAGGCGAGTATTGGGATTCCCTAAGTTATACGTATGGGGAAATGGATCGCAATCAAGATGCACACAGGCAGAGAATTGTTGATTGGATCAACGCTACTAATGGAACTTGTGGTGCATTTGATGTCACAACAAAAGGGATTCTCCATGCAGCGCTGGAAAGGTGCGAGTATTGGCGATTGTCAGATGAGAAGGGTAAGCCTCCAGGGGTTCTTGGATGGTGGCCATCTCGTGCTGTCACTTTCATAGAGAATCATGATACTGGTTCTACTCAGGGTCATTGGAGGTTTCCCAAAGGAAAAGAAATGCAAGGATATGCTTACATTTTGACTCATCCGGGAACACCTACAGTTTTCTATGACCACATTTTCTCTCACTACCAATCTGAAATTGCTGCTCTGATCTCTCTCAGAAACCGGAACAAGCTCAACTGTCGGAGTCTAGTTAAAATCACCAAGGCAGAAAGAGACGTGTATGCTGCCATCATTGATGAAAAGGTTGCCATAAAAATCGGACCAGGTCATTACGAACCTGCTAGTGGACCTCAAAATTGGAATAAAAGCCTCGAGGGAGGAGACTACAAGGTCTGGGAAGCAGCATTAGTTTTTTCCGCACCGGTATGTGTGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000272 GO:0003674 GO:0003824 GO:0004553 GO:0004556 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0005976 GO:0005982 GO:0005983 GO:0005984 GO:0005985 GO:0005987 GO:0006073 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009251 GO:0009311 GO:0009313 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009987 GO:0016052 GO:0016160 GO:0016787 GO:0016798 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044042 GO:0044237 GO:0044238 GO:0044247 GO:0044248 GO:0044260 GO:0044262 GO:0044264 GO:0044275 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046352 GO:0071704 GO:1901575

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Alpha-amyl_C2 Alpha-amylase

Protein Analysis

935

Amino Acids

104.68

Weight (kDa)

6.08

Isoelectric Point (pI)

41.25

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ig_N_CWD1	PF23166	108 - 234	1.8e-29	Alpha-glucan water dikinase 1 Ig-like N-terminal domain
Ig_N_CWD1	PF23166	302 - 419	4.4e-34	Alpha-glucan water dikinase 1 Ig-like N-terminal domain
Alpha-amylase	PF00128	558 - 626	8.6e-11	Alpha amylase, catalytic domain
Alpha-amyl_C2	PF07821	865 - 923	1.3e-18	Alpha-amylase C-terminal beta-sheet domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000937)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G69830
fragaria_vesca	FvH4_4g31910 FvH4_4g31910
malus_domestica	MD13G1078600.v1.1 MD16G1078200.v1.1
prunus_persica	Prupe.1G268300_v2.0.a1 Prupe.1G268300_v2.0.a1
pyrus_communis	pycom13g06820 pycom16g06770
rosa_chinensis	RchiOBHm_Chr1g0315221 RchiOBHm_Chr1g0339091 RchiOBHm_Chr4g0440561 RchiOBHm_Chr5g0021421 RchiOBHm_Chr6g0272311
rosa_laevigata	RLG00000006168 RLG00000015039 RLG00000019642 RLG00000030692
rosa_multiflora	Rmu_co8071990.1_g000001 Rmu_sc0000829.1_g000011 Rmu_sc0002387.1_g000011 Rmu_sc0011312.1_g000002
rosa_roxburghii	Rroxscaffold_4G00298760 Rroxscaffold_5G00381380 Rroxscaffold_6G00426500 Rroxscaffold_7G00169140 Rroxscaffold_7G00214180
rosa_rugosa	Rorug01G0004200 Rorug04G0326700 Rorug05G0356300 Rorug05G0477400 Rorug05G0527600 Rorug07G0284000
rosa_samantha	Rh1AG270700 Rh1AG270800 Rh1AG346400 Rh1BG260100 Rh1CG254500 Rh2AG416800 Rh2CG403000 Rh3DG074600 Rh3DG292400 Rh4AG190200 Rh4AG246600 Rh4AG378400 Rh4BG035500 Rh4BG391100 Rh4CG405800 Rh4DG085900 Rh4DG385000 Rh5AG156300 Rh5AG268600 Rh5AG480700 Rh5BG155700 Rh5CG169500 Rh6AG024000 Rh6BG089200 Rh6DG025600 Rh7CG251800
rosa_wichuraiana	Rw1G024120 Rw1G030800 Rw4G032400 Rw4G032500 Rw7G034860

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	2759
AbsI	CCTCGAGG	1 cut(s)	2741
Acc36I	ACCTGC	1 cut(s)	2716
Acc65I	GGTACC	1 cut(s)	1315
AccB1I	GGYRCC	1 cut(s)	1315
AccI	GTMKAC	2 cut(s)	41, 88
AciI	CCGC	8 cut(s)	106, 113, 187, 325, 837, 1070, 1111, 2787
AclWI	GGATC	5 cut(s)	287, 1425, 1952, 2232, 2273
AcoI	YGGCCR	3 cut(s)	21, 761, 2399
AcsI	RAATTY	4 cut(s)	631, 814, 915, 1056
AcuI	CTGAAG	5 cut(s)	1483, 1503, 1566, 1761, 1823
AfaI	GTAC	8 cut(s)	1216, 1284, 1317, 1330, 1351, 1652, 1748, 2093
AfeI	AGCGCT	1 cut(s)	2331
AfiI	CCNNNNNNNGG	5 cut(s)	67, 219, 224, 236, 2714
AflIII	ACRYGT	3 cut(s)	789, 872, 2646
AgeI	ACCGGT	1 cut(s)	2791
AgsI	TTSAA	6 cut(s)	547, 1096, 1201, 1260, 1298, 1460
AjiI	CACGTC	1 cut(s)	2647
AjnI	CCWGG	7 cut(s)	66, 218, 415, 763, 1754, 2380, 2692
AjuI	GAANNNNNNNTTGG	2 cut(s)	2245, 2277
AleI	CACNNNNGTG	1 cut(s)	1872
AloI	GAACNNNNNNTCC	6 cut(s)	411, 443, 1495, 1527, 1842, 1874
Alw21I	GWGCWC	1 cut(s)	1879
Alw26I	GTCTC	9 cut(s)	16, 181, 269, 490, 530, 956, 1802, 2637, 2745
Alw44I	GTGCAC	1 cut(s)	1875
AlwI	GGATC	5 cut(s)	287, 1425, 1952, 2232, 2273
AlwNI	CAGNNNCTG	4 cut(s)	195, 938, 1544, 1730
Ama87I	CYCGRG	2 cut(s)	15, 2741
Aor51HI	AGCGCT	1 cut(s)	2331
AoxI	GGCC	3 cut(s)	21, 761, 2399
ApaLI	GTGCAC	1 cut(s)	1875
ApoI	RAATTY	4 cut(s)	631, 814, 915, 1056
AseI	ATTAAT	1 cut(s)	1370
AsiGI	ACCGGT	1 cut(s)	2791
Asp700I	GAANNNNTTC	3 cut(s)	311, 1452, 1820
Asp718I	GGTACC	1 cut(s)	1315
AspLEI	GCGC	2 cut(s)	617, 2332
AspS9I	GGNCC	4 cut(s)	70, 227, 2690, 2717
AsuC2I	CCSGG	1 cut(s)	2514
AsuHPI	GGTGA	4 cut(s)	1405, 1728, 1859, 2620
AsuII	TTCGAA	1 cut(s)	1599
AvaI	CYCGRG	2 cut(s)	15, 2741
AvaII	GGWCC	4 cut(s)	70, 227, 2690, 2717
AxyI	CCTNAGG	1 cut(s)	1524
BaeGI	GKGCMC	2 cut(s)	959, 1879
BalI	TGGCCA	2 cut(s)	763, 2401
BanI	GGYRCC	1 cut(s)	1315
BanII	GRGCYC	2 cut(s)	60, 2169
BarI	GAAGNNNNNNTAC	2 cut(s)	1481, 1513
BauI	CACGAG	1 cut(s)	2406
BbsI	GAAGAC	1 cut(s)	314
Bbv12I	GWGCWC	1 cut(s)	1879
BccI	CCATC	9 cut(s)	305, 524, 1358, 1641, 2090, 2094, 2388, 2410, 2666
BceAI	ACGGC	2 cut(s)	8, 93
BciT130I	CCWGG	7 cut(s)	68, 220, 417, 765, 1756, 2382, 2694
BclI	TGATCA	1 cut(s)	2023
BcnI	CCSGG	1 cut(s)	2514
BcoDI	GTCTC	9 cut(s)	16, 181, 269, 490, 530, 956, 1802, 2637, 2745
BfaI	CTAG	7 cut(s)	170, 486, 1632, 1691, 2160, 2618, 2712
BfmI	CTRYAG	2 cut(s)	2178, 2523
BfoI	RGCGCY	1 cut(s)	2333
BfuAI	ACCTGC	1 cut(s)	2716
BglII	AGATCT	1 cut(s)	348
BlpI	GCTNAGC	1 cut(s)	137
BmcAI	AGTACT	1 cut(s)	1351
Bme1390I	CCNGG	8 cut(s)	68, 220, 417, 765, 1756, 2382, 2514, 2694
Bme18I	GGWCC	4 cut(s)	70, 227, 2690, 2717
BmeT110I	CYCGRG	2 cut(s)	15, 2741
BmgBI	CACGTC	1 cut(s)	2647
BmgT120I	GGNCC	4 cut(s)	70, 227, 2690, 2717
BmiI	GGNNCC	3 cut(s)	228, 229, 1317
BmrFI	CCNGG	8 cut(s)	68, 220, 417, 765, 1756, 2382, 2514, 2694
BmrI	ACTGGG	2 cut(s)	1030, 1633
BmsI	GCATC	6 cut(s)	1081, 1136, 1297, 1843, 2146, 2227
BmuI	ACTGGG	2 cut(s)	1030, 1633
BpiI	GAAGAC	1 cut(s)	314
BpmI	CTGGAG	3 cut(s)	50, 1762, 2364
Bpu10I	CCTNAGC	2 cut(s)	1311, 2075
Bpu1102I	GCTNAGC	1 cut(s)	137
Bpu14I	TTCGAA	1 cut(s)	1599
BpuEI	CTTGAG	2 cut(s)	305, 626
BpuMI	CCSGG	1 cut(s)	2514
BsaAI	YACGTR	4 cut(s)	873, 1218, 2152, 2211
BsaBI	GATNNNNATC	1 cut(s)	2229
BsaI	GGTCTC	3 cut(s)	269, 490, 1802
BsaJI	CCNNGG	7 cut(s)	218, 1421, 1612, 1754, 1755, 2381, 2631
BsaWI	WCCGGW	2 cut(s)	2592, 2791
BsaXI	ACNNNNNCTCC	2 cut(s)	1842, 1872
Bsc4I	CCNNNNNNNGG	5 cut(s)	67, 219, 224, 236, 2714
Bse118I	RCCGGY	1 cut(s)	2791
Bse1I	ACTGG	4 cut(s)	661, 1025, 1628, 2443
Bse21I	CCTNAGG	1 cut(s)	1524
Bse3DI	GCAATG	1 cut(s)	1533
Bse8I	GATNNNNATC	1 cut(s)	2229
BseBI	CCWGG	7 cut(s)	68, 220, 417, 765, 1756, 2382, 2694
BseDI	CCNNGG	7 cut(s)	218, 1421, 1612, 1754, 1755, 2381, 2631
BseJI	GATNNNNATC	1 cut(s)	2229
BseLI	CCNNNNNNNGG	5 cut(s)	67, 219, 224, 236, 2714
BseMI	GCAATG	1 cut(s)	1533
BseMII	CTCAG	7 cut(s)	21, 151, 951, 1325, 1515, 2462, 2599
BseNI	ACTGG	4 cut(s)	661, 1025, 1628, 2443
BseRI	GAGGAG	2 cut(s)	53, 2763
BseSI	GKGCMC	2 cut(s)	959, 1879
BseYI	CCCAGC	2 cut(s)	98, 1149
BsgI	GTGCAG	2 cut(s)	1659, 1962
Bsh1285I	CGRYCG	1 cut(s)	88
BshFI	GGCC	3 cut(s)	23, 763, 2401
BshNI	GGYRCC	1 cut(s)	1315
BshTI	ACCGGT	1 cut(s)	2791
BsiEI	CGRYCG	1 cut(s)	88
BsiHKAI	GWGCWC	1 cut(s)	1879
BsiHKCI	CYCGRG	2 cut(s)	15, 2741
BsiSI	CCGG	3 cut(s)	2513, 2593, 2792
BslFI	GGGAC	4 cut(s)	213, 1167, 1597, 2159
BslI	CCNNNNNNNGG	5 cut(s)	67, 219, 224, 236, 2714
BsmAI	GTCTC	9 cut(s)	16, 181, 269, 490, 530, 956, 1802, 2637, 2745
BsmBI	CGTCTC	1 cut(s)	2637
BsmFI	GGGAC	4 cut(s)	213, 1167, 1597, 2159
BsnI	GGCC	3 cut(s)	23, 763, 2401
Bso31I	GGTCTC	3 cut(s)	269, 490, 1802
BsoBI	CYCGRG	2 cut(s)	15, 2741
Bsp119I	TTCGAA	1 cut(s)	1599
Bsp1286I	GDGCHC	4 cut(s)	60, 959, 1879, 2169
Bsp1407I	TGTACA	1 cut(s)	1282
Bsp1720I	GCTNAGC	1 cut(s)	137
BspACI	CCGC	8 cut(s)	106, 113, 187, 325, 837, 1070, 1111, 2787
BspANI	GGCC	3 cut(s)	23, 763, 2401
BspCNI	CTCAG	7 cut(s)	20, 150, 952, 1324, 1516, 2461, 2598
BspHI	TCATGA	1 cut(s)	2431
BspLI	GGNNCC	3 cut(s)	228, 229, 1317
BspMI	ACCTGC	1 cut(s)	2716
BspPI	GGATC	5 cut(s)	287, 1425, 1952, 2232, 2273
BspQI	GCTCTTC	1 cut(s)	148
BspT104I	TTCGAA	1 cut(s)	1599
BspT107I	GGYRCC	1 cut(s)	1315
BspTNI	GGTCTC	3 cut(s)	269, 490, 1802
BsrDI	GCAATG	1 cut(s)	1533
BsrFI	RCCGGY	1 cut(s)	2791
BsrGI	TGTACA	1 cut(s)	1282
BsrI	ACTGG	4 cut(s)	661, 1025, 1628, 2443
BssAI	RCCGGY	1 cut(s)	2791
BssECI	CCNNGG	7 cut(s)	218, 1421, 1612, 1754, 1755, 2381, 2631
BssSI	CACGAG	1 cut(s)	2406
BssT1I	CCWWGG	3 cut(s)	1421, 1612, 2631
Bst2BI	CACGAG	1 cut(s)	2406
Bst2UI	CCWGG	7 cut(s)	68, 220, 417, 765, 1756, 2382, 2694
Bst6I	CTCTTC	1 cut(s)	148
BstAPI	GCANNNNNTGC	1 cut(s)	1949
BstAUI	TGTACA	1 cut(s)	1282
BstBAI	YACGTR	4 cut(s)	873, 1218, 2152, 2211
BstBI	TTCGAA	1 cut(s)	1599
BstC8I	GCNNGC	1 cut(s)	2072
BstENI	CCTNNNNNAGG	1 cut(s)	65
BstH2I	RGCGCY	1 cut(s)	2333
BstHHI	GCGC	2 cut(s)	617, 2332
BstMAI	GTCTC	9 cut(s)	16, 181, 269, 490, 530, 956, 1802, 2637, 2745
BstMCI	CGRYCG	1 cut(s)	88
BstMWI	GCNNNNNNNGC	4 cut(s)	623, 1448, 1484, 1949
BstNI	CCWGG	7 cut(s)	68, 220, 417, 765, 1756, 2382, 2694
BstNSI	RCATGY	2 cut(s)	760, 1753
BstSCI	CCNGG	8 cut(s)	66, 218, 415, 763, 1754, 2380, 2512, 2692
BstSFI	CTRYAG	2 cut(s)	2178, 2523
BstSLI	GKGCMC	2 cut(s)	959, 1879
BstSNI	TACGTA	1 cut(s)	2211
BstV2I	GAAGAC	1 cut(s)	314
BstX2I	RGATCY	2 cut(s)	348, 1430
BstXI	CCANNNNNNTGG	1 cut(s)	1832
BstYI	RGATCY	2 cut(s)	348, 1430
Bsu36I	CCTNAGG	1 cut(s)	1524
BsuRI	GGCC	3 cut(s)	23, 763, 2401
BtrI	CACGTC	1 cut(s)	2647
BtsI	GCAGTG	2 cut(s)	150, 847
BtsIMutI	CAGTG	6 cut(s)	150, 267, 847, 1018, 1676, 1698
BveI	ACCTGC	1 cut(s)	2716
Cac8I	GCNNGC	1 cut(s)	2072
CaiI	CAGNNNCTG	4 cut(s)	195, 938, 1544, 1730
CciI	TCATGA	1 cut(s)	2431
CfoI	GCGC	2 cut(s)	617, 2332
Cfr10I	RCCGGY	1 cut(s)	2791
Cfr13I	GGNCC	4 cut(s)	70, 227, 2690, 2717
CsiI	ACCWGGT	1 cut(s)	2692
Csp6I	GTAC	8 cut(s)	1215, 1283, 1316, 1329, 1350, 1651, 1747, 2092
CspAI	ACCGGT	1 cut(s)	2791
CspCI	CAANNNNNGTGG	2 cut(s)	1972, 2007
CviQI	GTAC	8 cut(s)	1215, 1283, 1316, 1329, 1350, 1651, 1747, 2092
DraI	TTTAAA	1 cut(s)	1926
DrdI	GACNNNNNNGTC	1 cut(s)	2759
DseDI	GACNNNNNNGTC	1 cut(s)	2759
EaeI	YGGCCR	3 cut(s)	21, 761, 2399
Eam1104I	CTCTTC	1 cut(s)	148
EarI	CTCTTC	1 cut(s)	148
Eco105I	TACGTA	1 cut(s)	2211
Eco130I	CCWWGG	3 cut(s)	1421, 1612, 2631
Eco24I	GRGCYC	2 cut(s)	60, 2169
Eco31I	GGTCTC	3 cut(s)	269, 490, 1802
Eco32I	GATATC	1 cut(s)	2053
Eco47I	GGWCC	4 cut(s)	70, 227, 2690, 2717
Eco47III	AGCGCT	1 cut(s)	2331
Eco57I	CTGAAG	5 cut(s)	1483, 1503, 1566, 1761, 1823
Eco81I	CCTNAGG	1 cut(s)	1524
Eco88I	CYCGRG	2 cut(s)	15, 2741
EcoNI	CCTNNNNNAGG	1 cut(s)	65
EcoO109I	RGGNCCY	2 cut(s)	70, 227
EcoRII	CCWGG	7 cut(s)	66, 218, 415, 763, 1754, 2380, 2692
EcoRV	GATATC	1 cut(s)	2053
EcoT14I	CCWWGG	3 cut(s)	1421, 1612, 2631
EcoT38I	GRGCYC	2 cut(s)	60, 2169
ErhI	CCWWGG	3 cut(s)	1421, 1612, 2631
Esp3I	CGTCTC	1 cut(s)	2637
FalI	AAGNNNNNCTT	4 cut(s)	296, 328, 1440, 1472
FaqI	GGGAC	4 cut(s)	213, 1167, 1597, 2159
FauI	CCCGC	1 cut(s)	1077
FauNDI	CATATG	1 cut(s)	1357
FbaI	TGATCA	1 cut(s)	2023
FblI	GTMKAC	2 cut(s)	41, 88
FriOI	GRGCYC	2 cut(s)	60, 2169
FspBI	CTAG	7 cut(s)	170, 486, 1632, 1691, 2160, 2618, 2712
GlaI	GCGC	2 cut(s)	616, 2331
GsaI	CCCAGC	2 cut(s)	102, 1153
GsuI	CTGGAG	3 cut(s)	50, 1762, 2364
HaeII	RGCGCY	1 cut(s)	2333
HaeIII	GGCC	3 cut(s)	23, 763, 2401
HapII	CCGG	3 cut(s)	2513, 2593, 2792
HhaI	GCGC	2 cut(s)	617, 2332
Hin6I	GCGC	2 cut(s)	615, 2330
HinP1I	GCGC	2 cut(s)	615, 2330
HincII	GTYRAC	2 cut(s)	42, 89
HindII	GTYRAC	2 cut(s)	42, 89
HpaII	CCGG	3 cut(s)	2513, 2593, 2792
HphI	GGTGA	4 cut(s)	1405, 1728, 1859, 2620
Hpy166II	GTNNAC	8 cut(s)	42, 89, 454, 925, 1018, 1161, 1877, 2717
Hpy188III	TCNNGA	9 cut(s)	15, 274, 827, 849, 1460, 1641, 1779, 2234, 2432
Hpy8I	GTNNAC	8 cut(s)	42, 89, 454, 925, 1018, 1161, 1877, 2717
Hpy99I	CGWCG	1 cut(s)	46
HpyCH4IV	ACGT	8 cut(s)	789, 872, 1217, 1401, 1830, 2151, 2210, 2646
HpyF10VI	GCNNNNNNNGC	4 cut(s)	623, 1448, 1484, 1949
HpySE526I	ACGT	8 cut(s)	789, 872, 1217, 1401, 1830, 2151, 2210, 2646
HspAI	GCGC	2 cut(s)	615, 2330
KflI	GGGWCCC	1 cut(s)	227
KpnI	GGTACC	1 cut(s)	1319
Ksp22I	TGATCA	1 cut(s)	2023
LguI	GCTCTTC	1 cut(s)	148
LmnI	GCTCC	1 cut(s)	737
LweI	GCATC	6 cut(s)	1081, 1136, 1297, 1843, 2146, 2227
MabI	ACCWGGT	1 cut(s)	2692
MaeI	CTAG	7 cut(s)	170, 486, 1632, 1691, 2160, 2618, 2712
MaeII	ACGT	8 cut(s)	789, 872, 1217, 1401, 1830, 2151, 2210, 2646
MaeIII	GTNAC	6 cut(s)	197, 683, 934, 1397, 2302, 2413
MfeI	CAATTG	2 cut(s)	618, 903
MflI	RGATCY	2 cut(s)	348, 1430
MhlI	GDGCHC	4 cut(s)	60, 959, 1879, 2169
MlsI	TGGCCA	2 cut(s)	763, 2401
MluNI	TGGCCA	2 cut(s)	763, 2401
MlyI	GAGTC	6 cut(s)	249, 1108, 1170, 1736, 2500, 2623
MmeI	TCCRAC	5 cut(s)	204, 715, 810, 1538, 2591
Mox20I	TGGCCA	2 cut(s)	763, 2401
MroXI	GAANNNNTTC	3 cut(s)	311, 1452, 1820
MscI	TGGCCA	2 cut(s)	763, 2401
MseI	TTAA	6 cut(s)	131, 603, 1370, 1620, 1925, 2622
MslI	CAYNNNNRTG	2 cut(s)	1872, 2794
Msp20I	TGGCCA	2 cut(s)	763, 2401
MspA1I	CMGCKG	3 cut(s)	503, 837, 1064
MspI	CCGG	3 cut(s)	2513, 2593, 2792
MspR9I	CCNGG	8 cut(s)	68, 220, 417, 765, 1756, 2382, 2514, 2694
MunI	CAATTG	2 cut(s)	618, 903
MvaI	CCWGG	7 cut(s)	68, 220, 417, 765, 1756, 2382, 2694
MwoI	GCNNNNNNNGC	4 cut(s)	623, 1448, 1484, 1949
NciI	CCSGG	1 cut(s)	2514
NdeI	CATATG	1 cut(s)	1357
NlaIV	GGNNCC	3 cut(s)	228, 229, 1317
NmuCI	GTSAC	4 cut(s)	683, 1397, 2302, 2413
NspI	RCATGY	2 cut(s)	760, 1753
NspV	TTCGAA	1 cut(s)	1599
OliI	CACNNNNGTG	1 cut(s)	1872
PaeR7I	CTCGAG	1 cut(s)	2741
PagI	TCATGA	1 cut(s)	2431
PasI	CCCWGGG	1 cut(s)	1755
PciSI	GCTCTTC	1 cut(s)	148
PcsI	WCGNNNNNNNCGW	1 cut(s)	279
PdmI	GAANNNNTTC	3 cut(s)	311, 1452, 1820
PflFI	GACNNNGTC	1 cut(s)	2694
PfoI	TCCNGGA	3 cut(s)	66, 415, 2512
PinAI	ACCGGT	1 cut(s)	2791
PleI	GAGTC	6 cut(s)	248, 1108, 1170, 1735, 2500, 2622
PpsI	GAGTC	6 cut(s)	248, 1108, 1170, 1735, 2500, 2622
Ppu21I	YACGTR	4 cut(s)	873, 1218, 2152, 2211
PpuMI	RGGWCCY	2 cut(s)	70, 227
PshBI	ATTAAT	1 cut(s)	1370
Psp5II	RGGWCCY	2 cut(s)	70, 227
Psp6I	CCWGG	7 cut(s)	66, 218, 415, 763, 1754, 2380, 2692
PspFI	CCCAGC	2 cut(s)	98, 1149
PspGI	CCWGG	7 cut(s)	66, 218, 415, 763, 1754, 2380, 2692
PspN4I	GGNNCC	3 cut(s)	228, 229, 1317
PspPI	GGNCC	4 cut(s)	70, 227, 2690, 2717
PspPPI	RGGWCCY	2 cut(s)	70, 227
PspXI	VCTCGAGB	1 cut(s)	2741
PsrI	GAACNNNNNNTAC	2 cut(s)	1766, 1798
PstNI	CAGNNNCTG	4 cut(s)	195, 938, 1544, 1730
PsuI	RGATCY	2 cut(s)	348, 1430
PsyI	GACNNNGTC	1 cut(s)	2694
PvuII	CAGCTG	2 cut(s)	503, 1064
RsaI	GTAC	8 cut(s)	1216, 1284, 1317, 1330, 1351, 1652, 1748, 2093
RsaNI	GTAC	8 cut(s)	1215, 1283, 1316, 1329, 1350, 1651, 1747, 2092
RseI	CAYNNNNRTG	2 cut(s)	1872, 2794
SalI	GTCGAC	2 cut(s)	40, 87
SapI	GCTCTTC	1 cut(s)	148
SaqAI	TTAA	6 cut(s)	131, 603, 1370, 1620, 1925, 2622
Sau96I	GGNCC	4 cut(s)	70, 227, 2690, 2717
ScaI	AGTACT	1 cut(s)	1351
SchI	GAGTC	6 cut(s)	249, 1108, 1170, 1736, 2500, 2623
ScrFI	CCNGG	8 cut(s)	68, 220, 417, 765, 1756, 2382, 2514, 2694
SduI	GDGCHC	4 cut(s)	60, 959, 1879, 2169
SexAI	ACCWGGT	1 cut(s)	2692
SfaNI	GCATC	6 cut(s)	1081, 1136, 1297, 1843, 2146, 2227
SfcI	CTRYAG	2 cut(s)	2178, 2523
Sfr274I	CTCGAG	1 cut(s)	2741
SfuI	TTCGAA	1 cut(s)	1599
SinI	GGWCC	4 cut(s)	70, 227, 2690, 2717
SlaI	CTCGAG	1 cut(s)	2741
SmiMI	CAYNNNNRTG	2 cut(s)	1872, 2794
SmlI	CTYRAG	3 cut(s)	320, 641, 2741
SmoI	CTYRAG	3 cut(s)	320, 641, 2741
SnaBI	TACGTA	1 cut(s)	2211
SsiI	CCGC	8 cut(s)	106, 113, 187, 325, 837, 1070, 1111, 2787
SspI	AATATT	1 cut(s)	1605
SspMI	CTAG	7 cut(s)	170, 486, 1632, 1691, 2160, 2618, 2712
StyD4I	CCNGG	8 cut(s)	66, 218, 415, 763, 1754, 2380, 2512, 2692
StyI	CCWWGG	3 cut(s)	1421, 1612, 2631
TaiI	ACGT	8 cut(s)	792, 875, 1220, 1404, 1833, 2154, 2213, 2649
TaqI	TCGA	7 cut(s)	41, 88, 119, 273, 1117, 1599, 2742
TatI	WGTACW	3 cut(s)	1282, 1328, 1349
TauI	GCSGC	2 cut(s)	189, 840
Tru1I	TTAA	6 cut(s)	131, 603, 1370, 1620, 1925, 2622
Tru9I	TTAA	6 cut(s)	131, 603, 1370, 1620, 1925, 2622
TscAI	CASTG	6 cut(s)	157, 274, 847, 1025, 1683, 1705
TseFI	GTSAC	4 cut(s)	683, 1397, 2302, 2413
Tsp45I	GTSAC	4 cut(s)	683, 1397, 2302, 2413
TspGWI	ACGGA	4 cut(s)	344, 934, 1338, 1800
TspRI	CASTG	6 cut(s)	157, 274, 847, 1025, 1683, 1705
Tth111I	GACNNNGTC	1 cut(s)	2694
VneI	GTGCAC	1 cut(s)	1875
VpaK11BI	GGWCC	4 cut(s)	70, 227, 2690, 2717
VspI	ATTAAT	1 cut(s)	1370
XagI	CCTNNNNNAGG	1 cut(s)	65
XapI	RAATTY	4 cut(s)	631, 814, 915, 1056
XceI	RCATGY	2 cut(s)	760, 1753
XhoI	CTCGAG	1 cut(s)	2741
XmiI	GTMKAC	2 cut(s)	41, 88
XmnI	GAANNNNTTC	3 cut(s)	311, 1452, 1820
XspI	CTAG	7 cut(s)	170, 486, 1632, 1691, 2160, 2618, 2712
ZrmI	AGTACT	1 cut(s)	1351

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.