Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr4

Orthogroup ID

OG0000937

Physical Location & Seq

Genomic Coordinates Reverse (-)

59604304 .. 59612005

Gene Structure 7702 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw4G032500.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2703 bp

ATGTCGACGGTGAGCATTGATCCTCTTCTCCACCACCACTGTCGCCGGGGAAACTCCCGGCACCGTTCAACGCCGAAGCTCATCCACCTCAGCTCCTCCCTGAGTGTCTTTCCGAAAAAGTTCGAGTTTCGTGGCCGGAGCTTCTGCAACTTCCGGCATCGCACTGCTCTCACTCTCAGAGCTTCTTCTACTGACACCGCCGTAGCTGCGACCACCGCGCCTTTCTTCAAGGAAACATTTCCTCTTGAGCTAACTGAACTGGTGGAGGGAAAGATCTTTGTGAGGTTAGATCACGGGAAGAAGGCTCGGAATTGGATTCTCACTGTTGGTTGTACTCTTCCCGGAAACTGGATTCTGCACTGGGGAGTTTCACATGTTGATGACGTCGGCAGTGAATGGGATCAACCTCCTGATGAAATGAGGCCACCTGGTTCAATTCCAATTAAGGACTATGCAATAGAAACACCCTTGAAGAAATCAGCATCATCTGTTGGAGATGATACCTCTCAGGAAGTGAAGATTGATTTTCATCTTGACGGTGCAATTGCAGCCATAAATTTCGTTCTAAAGGATGAAGAAACTGGCACTTGGTATCAGTACAAAGGAAGAGATTTCAAAGTGCCTCTTGTGGGCTACCTGCAAGAATATGGCAATGTTGTTGGAGCAAAAAAGGGAGTGGGGATGTTGCCAGGAGCTTTGGGGAAGTTATCCAACTTATTTTTCAAGGCAGAATCATCAAACTCCCAAGATAAAAATAGCAGTAGTGAATCCAGAGGAACTAAAGAGGAAACTAGGTCTCTAGAAGGGTTCTACGAAGAACTCCCTATCGCAAAACAAATTGCTGTAGTGAATTCAGTAACTGTTTCTGTCAGGAAGTGCCCTGAGACAGCTAAGAATCTTCTATATTTGGAAACGGATTTACCTGATCATGTTGTTGTTCATTGGGGAGTTTGCAAAGATGATGCTAAAAGATGGGAAATTCCAGCAGCCCCACATCCGCCAGAAACAGTAGTGTTCAAGGATAAAGCTTTGCGAACTCGACTGCAGCAAAAAGAGGGAGCAAATGGACGTTGGGCATTGTTTACTTTAGATGAAGGAACCGCAGGATTTCTTTTTGTTTTCAAGCTAAGTGAAAGTACATGGTTGAAATGTAAGGGCAATGACTTCTACATCCCCCTTTCAAGTGCAAATAATTCACCTGCTGCAGCAAAAGAGGATCAATCTGAAGGTGCTAATGTAGATGAGAGAAGTGCAGAAAAAATAGATGAAAGTTCTTTTACTGAATATACCAATGGAATAATAAATGAAATAAGGACCCTGGTGAGTGGCATTTCCTCTGAGAAGAGACGAAAGACAAAATCCAAAGAAGCACAAGAAAGCATTCTTCAAGAAATAGAAAAATTGGCTGCTGAAGCCTATAGTATCTTCAGAAGCACTGTTCCCACATTTACTGAGGAAGCTATTTCAGAAACTGAAGAATTACCACCCTCTGTGAAAATAATCTCAGGGACAGGCACGGGGTTTGAAATATTGTGCCAAGGTTTTAATTGGGAATCTCATAAATCTGGAAGATGGTACATGGAGCTCAAAGATAAAGCTGCTGAATTATCTGCACTAGGTTTCACTGTGATTTGGTTACCACCCCCTACAGATTCTGTGTCACCTGAAGGGTACATGCCAACGGATTTGTATAATCTGAACTGCAGATATGGCACTATGGATGAACTGAAGGAGACTGTGAAGGCATTCCATAAAGTTGGTATCAAAGTACTTGGAGATGTTGTTCTAAATCACCGCTGTGCCCAGTATCAGAATAGCAATGGTGTATGGAATATATTTGGTGGTCGTTTAAATTGGGATGATCGTGCAGTTGTTGCAGATGATCCGCATTTTCAGGGTAGGGGCAACAAAAGTAGTGGAGATAGTTTTCATGCTGCACCAAACATTGATCATTCACAAGAGTTTGTGAGGAAGGATATCAAAGAATGGTTATGCTGGCTAAGGCAAGAAATTGGGTTTGATGGATGGAGGCTTGATTTTGTTCGAGGATTTTGGGGTGGTTATGTTAAGGACTACATGGATGCTAGCGAGCCATACTTTGCTGTTGGTGAGTTTTGGGATTCCCTAAGTTATACGTATGGTGAGATGGATCACAATCAAGATGCACATAGGCAGAGAATTATTGATTGGATTAATGCTACTAGTGGAGCCGCTGGTGCTTTTGATGTAACAACAAAAGGCATCCTTCATGCTGCGTTGGAAAGATGTGAGTATTGGCGATTATCGGATCAGAAGGGAAAACCTCCTGGGGTTGTTGGATGGTGGCCATCTCGTGCTGTTACCTTCATAGAGAATCACGATACTGGTTCTACTCAGGGTCACTGGAGGTTTCCACATGACAAGGAAATTCAAGGATATGCATACATTCTGACTCATCCAGGAACACCTGCAGTCTTCTATGACCACATCTTCTCACACTACAGATCTGAAATTGCAGGTCTAATCTCTCTGAGAAACCGGAACACAATCAACTGTCGGAGCATAGTTGAAATTACCAAAGCTGAAAGAGATGTGTATGCCGCTATAATTGATGAAAAAGTAGCAATGAAGATCGGACCAGGTCACTATGAACCCCCAAATGGGCCTAAAAAATGGTCCAAAAGCCTCGAGGGAAGAGACTACAAGGTCTGGGAAGCATCTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000272 GO:0003674 GO:0003824 GO:0004553 GO:0004556 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0005976 GO:0005982 GO:0005983 GO:0005984 GO:0005985 GO:0005987 GO:0006073 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009251 GO:0009311 GO:0009313 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009987 GO:0016052 GO:0016160 GO:0016787 GO:0016798 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044042 GO:0044237 GO:0044238 GO:0044247 GO:0044248 GO:0044260 GO:0044262 GO:0044264 GO:0044275 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046352 GO:0071704 GO:1901575

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Alpha-amyl_C2 Alpha-amylase

Protein Analysis

900

Amino Acids

101.3

Weight (kDa)

6.16

Isoelectric Point (pI)

41.38

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ig_N_CWD1	PF23166	88 - 209	9.5e-30	Alpha-glucan water dikinase 1 Ig-like N-terminal domain
Ig_N_CWD1	PF23166	276 - 393	2.9e-34	Alpha-glucan water dikinase 1 Ig-like N-terminal domain
Alpha-amylase	PF00128	536 - 603	1.1e-12	Alpha amylase, catalytic domain
Alpha-amyl_C2	PF07821	840 - 898	7.7e-20	Alpha-amylase C-terminal beta-sheet domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Orthologous Genes (Group: OG0000937)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G69830
fragaria_vesca	FvH4_4g31910 FvH4_4g31910
malus_domestica	MD13G1078600.v1.1 MD16G1078200.v1.1
prunus_persica	Prupe.1G268300_v2.0.a1 Prupe.1G268300_v2.0.a1
pyrus_communis	pycom13g06820 pycom16g06770
rosa_chinensis	RchiOBHm_Chr1g0315221 RchiOBHm_Chr1g0339091 RchiOBHm_Chr4g0440561 RchiOBHm_Chr5g0021421 RchiOBHm_Chr6g0272311
rosa_laevigata	RLG00000006168 RLG00000015039 RLG00000019642 RLG00000030692
rosa_multiflora	Rmu_co8071990.1_g000001 Rmu_sc0000829.1_g000011 Rmu_sc0002387.1_g000011 Rmu_sc0011312.1_g000002
rosa_roxburghii	Rroxscaffold_4G00298760 Rroxscaffold_5G00381380 Rroxscaffold_6G00426500 Rroxscaffold_7G00169140 Rroxscaffold_7G00214180
rosa_rugosa	Rorug01G0004200 Rorug04G0326700 Rorug05G0356300 Rorug05G0477400 Rorug05G0527600 Rorug07G0284000
rosa_samantha	Rh1AG270700 Rh1AG270800 Rh1AG346400 Rh1BG260100 Rh1CG254500 Rh2AG416800 Rh2CG403000 Rh3DG074600 Rh3DG292400 Rh4AG190200 Rh4AG246600 Rh4AG378400 Rh4BG035500 Rh4BG391100 Rh4CG405800 Rh4DG085900 Rh4DG385000 Rh5AG156300 Rh5AG268600 Rh5AG480700 Rh5BG155700 Rh5CG169500 Rh6AG024000 Rh6BG089200 Rh6DG025600 Rh7CG251800
rosa_wichuraiana	Rw1G024120 Rw1G030800 Rw4G032400 Rw4G032500 Rw7G034860

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	2 cut(s)	1207, 2455
AasI	GACNNNNNNGTC	1 cut(s)	2684
AatII	GACGTC	1 cut(s)	387
AbsI	CCTCGAGG	1 cut(s)	2666
Acc36I	ACCTGC	4 cut(s)	645, 1207, 2455, 2486
AccB1I	GGYRCC	1 cut(s)	60
AccI	GTMKAC	1 cut(s)	5
AccII	CGCG	1 cut(s)	218
AciI	CCGC	8 cut(s)	198, 216, 998, 1101, 1795, 1886, 2211, 2580
AclWI	GGATC	6 cut(s)	14, 408, 1224, 1877, 2157, 2295
AcoI	YGGCCR	2 cut(s)	133, 2324
AcsI	RAATTY	4 cut(s)	556, 850, 978, 2406
AcuI	CTGAAG	6 cut(s)	1245, 1411, 1431, 1494, 1686, 1748
AcyI	GRCGYC	1 cut(s)	384
AfaI	GTAC	6 cut(s)	334, 599, 1138, 1577, 1673, 1770
AfiI	CCNNNNNNNGG	4 cut(s)	348, 629, 2639, 2640
AflIII	ACRYGT	1 cut(s)	373
AhlI	ACTAGT	1 cut(s)	2201
AjnI	CCWGG	6 cut(s)	427, 688, 1317, 2305, 2437, 2617
AjuI	GAANNNNNNNTTGG	4 cut(s)	704, 736, 2170, 2202
AleI	CACNNNNGTG	1 cut(s)	1797
AloI	GAACNNNNNNTCC	2 cut(s)	1767, 1799
Alw21I	GWGCWC	1 cut(s)	1587
Alw26I	GTCTC	5 cut(s)	801, 878, 1339, 1727, 2670
AlwI	GGATC	6 cut(s)	14, 408, 1224, 1877, 2157, 2295
AlwNI	CAGNNNCTG	3 cut(s)	860, 1472, 1655
Ama87I	CYCGRG	1 cut(s)	2666
AoxI	GGCC	4 cut(s)	133, 422, 2324, 2642
ApoI	RAATTY	4 cut(s)	556, 850, 978, 2406
AseI	ATTAAT	1 cut(s)	2193
Asp700I	GAANNNNTTC	4 cut(s)	119, 237, 1380, 1745
AspLEI	GCGC	1 cut(s)	220
AspS9I	GGNCC	4 cut(s)	1314, 2615, 2642, 2655
AsuC2I	CCSGG	3 cut(s)	47, 58, 342
AsuHPI	GGTGA	7 cut(s)	22, 1188, 1333, 1653, 1784, 2120, 2153
AsuNHI	GCTAGC	1 cut(s)	2084
AvaI	CYCGRG	1 cut(s)	2666
AvaII	GGWCC	3 cut(s)	1314, 2615, 2655
BaeGI	GKGCMC	2 cut(s)	881, 1804
BalI	TGGCCA	1 cut(s)	2326
BanI	GGYRCC	1 cut(s)	60
BanII	GRGCYC	1 cut(s)	1587
BauI	CACGAG	1 cut(s)	2331
BbsI	GAAGAC	1 cut(s)	2446
Bbv12I	GWGCWC	1 cut(s)	1587
BbvCI	CCTCAGC	1 cut(s)	89
BccI	CCATC	7 cut(s)	966, 1566, 2015, 2019, 2140, 2313, 2335
BceAI	ACGGC	1 cut(s)	185
BciT130I	CCWGG	6 cut(s)	429, 690, 1319, 2307, 2439, 2619
BclI	TGATCA	2 cut(s)	925, 1948
BcnI	CCSGG	3 cut(s)	47, 58, 342
BcoDI	GTCTC	5 cut(s)	801, 878, 1339, 1727, 2670
BcuI	ACTAGT	1 cut(s)	2201
BfaI	CTAG	5 cut(s)	792, 800, 1616, 2085, 2202
BfmI	CTRYAG	8 cut(s)	843, 1043, 1203, 1417, 1647, 1702, 2448, 2479
BfuAI	ACCTGC	4 cut(s)	645, 1207, 2455, 2486
BglII	AGATCT	2 cut(s)	273, 2483
BmcAI	AGTACT	1 cut(s)	1770
Bme1390I	CCNGG	9 cut(s)	47, 58, 342, 429, 690, 1319, 2307, 2439, 2619
Bme18I	GGWCC	3 cut(s)	1314, 2615, 2655
BmeT110I	CYCGRG	1 cut(s)	2666
BmgT120I	GGNCC	4 cut(s)	1314, 2615, 2642, 2655
BmiI	GGNNCC	4 cut(s)	62, 1099, 1316, 2209
BmrFI	CCNGG	9 cut(s)	47, 58, 342, 429, 690, 1319, 2307, 2439, 2619
BmrI	ACTGGG	2 cut(s)	370, 1798
BmsI	GCATC	6 cut(s)	166, 491, 952, 2071, 2152, 2250
BmtI	GCTAGC	1 cut(s)	2088
BmuI	ACTGGG	2 cut(s)	370, 1798
BpiI	GAAGAC	1 cut(s)	2446
BpmI	CTGGAG	1 cut(s)	2404
Bpu10I	CCTNAGC	2 cut(s)	89, 2000
BpuEI	CTTGAG	1 cut(s)	266
BpuMI	CCSGG	3 cut(s)	47, 58, 342
BsaAI	YACGTR	1 cut(s)	2136
BsaBI	GATNNNNATC	2 cut(s)	528, 2154
BsaHI	GRCGYC	1 cut(s)	384
BsaI	GGTCTC	1 cut(s)	801
BsaJI	CCNNGG	4 cut(s)	46, 1317, 1537, 2306
BsaWI	WCCGGW	1 cut(s)	2517
BsaXI	ACNNNNNCTCC	4 cut(s)	77, 107, 1767, 1797
Bsc4I	CCNNNNNNNGG	4 cut(s)	348, 629, 2639, 2640
Bse1I	ACTGG	7 cut(s)	264, 353, 365, 586, 1804, 2368, 2387
Bse3DI	GCAATG	4 cut(s)	658, 1165, 1825, 2610
Bse8I	GATNNNNATC	2 cut(s)	528, 2154
BseBI	CCWGG	6 cut(s)	429, 690, 1319, 2307, 2439, 2619
BseDI	CCNNGG	4 cut(s)	46, 1317, 1537, 2306
BseJI	GATNNNNATC	2 cut(s)	528, 2154
BseLI	CCNNNNNNNGG	4 cut(s)	348, 629, 2639, 2640
BseMI	GCAATG	4 cut(s)	658, 1165, 1825, 2610
BseNI	ACTGG	7 cut(s)	264, 353, 365, 586, 1804, 2368, 2387
BseRI	GAGGAG	1 cut(s)	85
BseSI	GKGCMC	2 cut(s)	881, 1804
BsgI	GTGCAG	5 cut(s)	341, 1272, 1596, 1887, 1920
Bsh1236I	CGCG	1 cut(s)	218
BshFI	GGCC	4 cut(s)	135, 424, 2326, 2644
BshNI	GGYRCC	1 cut(s)	60
BsiHKAI	GWGCWC	1 cut(s)	1587
BsiHKCI	CYCGRG	1 cut(s)	2666
BsiSI	CCGG	6 cut(s)	46, 58, 136, 154, 342, 2518
BslFI	GGGAC	1 cut(s)	1522
BslI	CCNNNNNNNGG	4 cut(s)	348, 629, 2639, 2640
BsmAI	GTCTC	5 cut(s)	801, 878, 1339, 1727, 2670
BsmBI	CGTCTC	1 cut(s)	1339
BsmFI	GGGAC	1 cut(s)	1522
BsmI	GAATGC	2 cut(s)	1380, 1745
BsnI	GGCC	4 cut(s)	135, 424, 2326, 2644
Bso31I	GGTCTC	1 cut(s)	801
BsoBI	CYCGRG	1 cut(s)	2666
Bsp1286I	GDGCHC	3 cut(s)	881, 1587, 1804
BspACI	CCGC	8 cut(s)	198, 216, 998, 1101, 1795, 1886, 2211, 2580
BspANI	GGCC	4 cut(s)	135, 424, 2326, 2644
BspFNI	CGCG	1 cut(s)	218
BspLI	GGNNCC	4 cut(s)	62, 1099, 1316, 2209
BspMAI	CTGCAG	4 cut(s)	1047, 1207, 1706, 2452
BspMI	ACCTGC	4 cut(s)	645, 1207, 2455, 2486
BspOI	GCTAGC	1 cut(s)	2088
BspPI	GGATC	6 cut(s)	14, 408, 1224, 1877, 2157, 2295
BspT107I	GGYRCC	1 cut(s)	60
BspTNI	GGTCTC	1 cut(s)	801
BsrDI	GCAATG	4 cut(s)	658, 1165, 1825, 2610
BsrI	ACTGG	7 cut(s)	264, 353, 365, 586, 1804, 2368, 2387
BssECI	CCNNGG	4 cut(s)	46, 1317, 1537, 2306
BssNI	GRCGYC	1 cut(s)	384
BssSI	CACGAG	1 cut(s)	2331
BssT1I	CCWWGG	1 cut(s)	1537
Bst2BI	CACGAG	1 cut(s)	2331
Bst2UI	CCWGG	6 cut(s)	429, 690, 1319, 2307, 2439, 2619
Bst6I	CTCTTC	5 cut(s)	30, 342, 601, 1337, 2668
BstACI	GRCGYC	1 cut(s)	384
BstAPI	GCANNNNNTGC	1 cut(s)	1874
BstBAI	YACGTR	1 cut(s)	2136
BstC8I	GCNNGC	3 cut(s)	1997, 2086, 2090
BstEII	GGTNACC	1 cut(s)	1635
BstFNI	CGCG	1 cut(s)	218
BstHHI	GCGC	1 cut(s)	220
BstMAI	GTCTC	5 cut(s)	801, 878, 1339, 1727, 2670
BstMWI	GCNNNNNNNGC	7 cut(s)	206, 215, 548, 1412, 1710, 1874, 2216
BstNI	CCWGG	6 cut(s)	429, 690, 1319, 2307, 2439, 2619
BstNSI	RCATGY	2 cut(s)	377, 1678
BstPI	GGTNACC	1 cut(s)	1635
BstSCI	CCNGG	9 cut(s)	45, 56, 340, 427, 688, 1317, 2305, 2437, 2617
BstSFI	CTRYAG	8 cut(s)	843, 1043, 1203, 1417, 1647, 1702, 2448, 2479
BstSLI	GKGCMC	2 cut(s)	881, 1804
BstSNI	TACGTA	1 cut(s)	2136
BstUI	CGCG	1 cut(s)	218
BstV2I	GAAGAC	1 cut(s)	2446
BstX2I	RGATCY	2 cut(s)	273, 2483
BstXI	CCANNNNNNTGG	1 cut(s)	1757
BstYI	RGATCY	2 cut(s)	273, 2483
BsuRI	GGCC	4 cut(s)	135, 424, 2326, 2644
BtgZI	GCGATG	1 cut(s)	143
BtsI	GCAGTG	2 cut(s)	162, 397
BtsIMutI	CAGTG	8 cut(s)	37, 162, 321, 358, 397, 1434, 1623, 2380
BveI	ACCTGC	4 cut(s)	645, 1207, 2455, 2486
Cac8I	GCNNGC	3 cut(s)	1997, 2086, 2090
CaiI	CAGNNNCTG	3 cut(s)	860, 1472, 1655
CfoI	GCGC	1 cut(s)	220
Cfr13I	GGNCC	4 cut(s)	1314, 2615, 2642, 2655
CsiI	ACCWGGT	2 cut(s)	427, 2617
Csp6I	GTAC	6 cut(s)	333, 598, 1137, 1576, 1672, 1769
CspCI	CAANNNNNGTGG	2 cut(s)	1897, 1932
CviAII	CATG	9 cut(s)	374, 929, 1140, 1579, 1675, 1931, 2077, 2249, 2396
CviQI	GTAC	6 cut(s)	333, 598, 1137, 1576, 1672, 1769
DraI	TTTAAA	1 cut(s)	1851
DrdI	GACNNNNNNGTC	1 cut(s)	2684
DseDI	GACNNNNNNGTC	1 cut(s)	2684
EaeI	YGGCCR	2 cut(s)	133, 2324
Eam1104I	CTCTTC	5 cut(s)	30, 342, 601, 1337, 2668
EarI	CTCTTC	5 cut(s)	30, 342, 601, 1337, 2668
EciI	GGCGGA	1 cut(s)	987
Ecl136II	GAGCTC	1 cut(s)	1585
Eco105I	TACGTA	1 cut(s)	2136
Eco130I	CCWWGG	1 cut(s)	1537
Eco24I	GRGCYC	1 cut(s)	1587
Eco31I	GGTCTC	1 cut(s)	801
Eco32I	GATATC	1 cut(s)	1978
Eco47I	GGWCC	3 cut(s)	1314, 2615, 2655
Eco53kI	GAGCTC	1 cut(s)	1585
Eco57I	CTGAAG	6 cut(s)	1245, 1411, 1431, 1494, 1686, 1748
Eco88I	CYCGRG	1 cut(s)	2666
Eco91I	GGTNACC	1 cut(s)	1635
EcoICRI	GAGCTC	1 cut(s)	1585
EcoO109I	RGGNCCY	1 cut(s)	1314
EcoO65I	GGTNACC	1 cut(s)	1635
EcoRI	GAATTC	1 cut(s)	850
EcoRII	CCWGG	6 cut(s)	427, 688, 1317, 2305, 2437, 2617
EcoRV	GATATC	1 cut(s)	1978
EcoT14I	CCWWGG	1 cut(s)	1537
EcoT22I	ATGCAT	1 cut(s)	2422
EcoT38I	GRGCYC	1 cut(s)	1587
ErhI	CCWWGG	1 cut(s)	1537
Esp3I	CGTCTC	1 cut(s)	1339
FaeI	CATG	9 cut(s)	377, 932, 1143, 1582, 1678, 1934, 2080, 2252, 2399
FalI	AAGNNNNNCTT	4 cut(s)	609, 641, 2229, 2261
FaqI	GGGAC	1 cut(s)	1522
FatI	CATG	9 cut(s)	373, 928, 1139, 1578, 1674, 1930, 2076, 2248, 2395
FbaI	TGATCA	2 cut(s)	925, 1948
FblI	GTMKAC	1 cut(s)	5
FriOI	GRGCYC	1 cut(s)	1587
FspBI	CTAG	5 cut(s)	792, 800, 1616, 2085, 2202
GlaI	GCGC	1 cut(s)	219
GsuI	CTGGAG	1 cut(s)	2404
HaeIII	GGCC	4 cut(s)	135, 424, 2326, 2644
HapII	CCGG	6 cut(s)	46, 58, 136, 154, 342, 2518
HhaI	GCGC	1 cut(s)	220
Hin1I	GRCGYC	1 cut(s)	384
Hin1II	CATG	9 cut(s)	377, 932, 1143, 1582, 1678, 1934, 2080, 2252, 2399
Hin6I	GCGC	1 cut(s)	218
HinP1I	GCGC	1 cut(s)	218
HincII	GTYRAC	1 cut(s)	6
HindII	GTYRAC	1 cut(s)	6
HindIII	AAGCTT	1 cut(s)	1026
HpaII	CCGG	6 cut(s)	46, 58, 136, 154, 342, 2518
HphI	GGTGA	7 cut(s)	22, 1188, 1333, 1653, 1784, 2120, 2153
Hpy166II	GTNNAC	2 cut(s)	6, 1083
Hpy8I	GTNNAC	2 cut(s)	6, 1083
Hpy99I	CGWCG	2 cut(s)	10, 389
HpyCH4IV	ACGT	3 cut(s)	384, 1069, 2135
HpyF10VI	GCNNNNNNNGC	7 cut(s)	206, 215, 548, 1412, 1710, 1874, 2216
HpySE526I	ACGT	3 cut(s)	384, 1069, 2135
Hsp92I	GRCGYC	1 cut(s)	384
Hsp92II	CATG	9 cut(s)	377, 932, 1143, 1582, 1678, 1934, 2080, 2252, 2399
HspAI	GCGC	1 cut(s)	218
Ksp22I	TGATCA	2 cut(s)	925, 1948
LmnI	GCTCC	8 cut(s)	98, 138, 662, 692, 1058, 1582, 2207, 2538
LweI	GCATC	6 cut(s)	166, 491, 952, 2071, 2152, 2250
MabI	ACCWGGT	2 cut(s)	427, 2617
MaeI	CTAG	5 cut(s)	792, 800, 1616, 2085, 2202
MaeII	ACGT	3 cut(s)	384, 1069, 2135
MaeIII	GTNAC	7 cut(s)	856, 1635, 1659, 2227, 2338, 2378, 2621
MfeI	CAATTG	1 cut(s)	543
MflI	RGATCY	2 cut(s)	273, 2483
MhlI	GDGCHC	3 cut(s)	881, 1587, 1804
MlsI	TGGCCA	1 cut(s)	2326
MluNI	TGGCCA	1 cut(s)	2326
MlyI	GAGTC	1 cut(s)	2425
MmeI	TCCRAC	6 cut(s)	472, 640, 735, 2238, 2296, 2516
Mox20I	TGGCCA	1 cut(s)	2326
Mph1103I	ATGCAT	1 cut(s)	2422
MroXI	GAANNNNTTC	4 cut(s)	119, 237, 1380, 1745
MscI	TGGCCA	1 cut(s)	2326
MseI	TTAA	6 cut(s)	444, 1545, 1850, 2067, 2193, 2701
MslI	CAYNNNNRTG	2 cut(s)	378, 1797
Msp20I	TGGCCA	1 cut(s)	2326
MspA1I	CMGCKG	2 cut(s)	1797, 2213
MspI	CCGG	6 cut(s)	46, 58, 136, 154, 342, 2518
MspR9I	CCNGG	9 cut(s)	47, 58, 342, 429, 690, 1319, 2307, 2439, 2619
MunI	CAATTG	1 cut(s)	543
Mva1269I	GAATGC	2 cut(s)	1380, 1745
MvaI	CCWGG	6 cut(s)	429, 690, 1319, 2307, 2439, 2619
MvnI	CGCG	1 cut(s)	218
MwoI	GCNNNNNNNGC	7 cut(s)	206, 215, 548, 1412, 1710, 1874, 2216
NciI	CCSGG	3 cut(s)	47, 58, 342
NheI	GCTAGC	1 cut(s)	2084
NlaIII	CATG	9 cut(s)	377, 932, 1143, 1582, 1678, 1934, 2080, 2252, 2399
NlaIV	GGNNCC	4 cut(s)	62, 1099, 1316, 2209
NmuCI	GTSAC	3 cut(s)	1659, 2378, 2621
NsiI	ATGCAT	1 cut(s)	2422
NspI	RCATGY	2 cut(s)	377, 1678
OliI	CACNNNNGTG	1 cut(s)	1797
PaeR7I	CTCGAG	1 cut(s)	2666
PaqCI	CACCTGC	2 cut(s)	1207, 2455
PciI	ACATGT	1 cut(s)	373
PctI	GAATGC	2 cut(s)	1380, 1745
PdmI	GAANNNNTTC	4 cut(s)	119, 237, 1380, 1745
PfeI	GAWTC	9 cut(s)	316, 352, 731, 767, 895, 1553, 1652, 2120, 2353
PflFI	GACNNNGTC	1 cut(s)	2619
PfoI	TCCNGGA	2 cut(s)	340, 2437
PleI	GAGTC	1 cut(s)	2425
PpsI	GAGTC	1 cut(s)	2425
Ppu21I	YACGTR	1 cut(s)	2136
PpuMI	RGGWCCY	1 cut(s)	1314
PscI	ACATGT	1 cut(s)	373
PshBI	ATTAAT	1 cut(s)	2193
Psp124BI	GAGCTC	1 cut(s)	1587
Psp5II	RGGWCCY	1 cut(s)	1314
Psp6I	CCWGG	6 cut(s)	427, 688, 1317, 2305, 2437, 2617
PspEI	GGTNACC	1 cut(s)	1635
PspGI	CCWGG	6 cut(s)	427, 688, 1317, 2305, 2437, 2617
PspN4I	GGNNCC	4 cut(s)	62, 1099, 1316, 2209
PspPI	GGNCC	4 cut(s)	1314, 2615, 2642, 2655
PspPPI	RGGWCCY	1 cut(s)	1314
PspXI	VCTCGAGB	1 cut(s)	2666
PstI	CTGCAG	4 cut(s)	1047, 1207, 1706, 2452
PstNI	CAGNNNCTG	3 cut(s)	860, 1472, 1655
PsuI	RGATCY	2 cut(s)	273, 2483
PsyI	GACNNNGTC	1 cut(s)	2619
RsaI	GTAC	6 cut(s)	334, 599, 1138, 1577, 1673, 1770
RsaNI	GTAC	6 cut(s)	333, 598, 1137, 1576, 1672, 1769
RseI	CAYNNNNRTG	2 cut(s)	378, 1797
SacI	GAGCTC	1 cut(s)	1587
SalI	GTCGAC	1 cut(s)	4
SaqAI	TTAA	6 cut(s)	444, 1545, 1850, 2067, 2193, 2701
Sau96I	GGNCC	4 cut(s)	1314, 2615, 2642, 2655
ScaI	AGTACT	1 cut(s)	1770
SchI	GAGTC	1 cut(s)	2425
ScrFI	CCNGG	9 cut(s)	47, 58, 342, 429, 690, 1319, 2307, 2439, 2619
SduI	GDGCHC	3 cut(s)	881, 1587, 1804
SexAI	ACCWGGT	2 cut(s)	427, 2617
SfaNI	GCATC	6 cut(s)	166, 491, 952, 2071, 2152, 2250
SfcI	CTRYAG	8 cut(s)	843, 1043, 1203, 1417, 1647, 1702, 2448, 2479
Sfr274I	CTCGAG	1 cut(s)	2666
SinI	GGWCC	3 cut(s)	1314, 2615, 2655
SlaI	CTCGAG	1 cut(s)	2666
SmiMI	CAYNNNNRTG	2 cut(s)	378, 1797
SmlI	CTYRAG	2 cut(s)	245, 2666
SmoI	CTYRAG	2 cut(s)	245, 2666
SnaBI	TACGTA	1 cut(s)	2136
SpeI	ACTAGT	1 cut(s)	2201
SsiI	CCGC	8 cut(s)	198, 216, 998, 1101, 1795, 1886, 2211, 2580
SspI	AATATT	1 cut(s)	1530
SspMI	CTAG	5 cut(s)	792, 800, 1616, 2085, 2202
SstI	GAGCTC	1 cut(s)	1587
StyD4I	CCNGG	9 cut(s)	45, 56, 340, 427, 688, 1317, 2305, 2437, 2617
StyI	CCWWGG	1 cut(s)	1537
TaiI	ACGT	3 cut(s)	387, 1072, 2138
TaqI	TCGA	5 cut(s)	5, 123, 1039, 2044, 2667
TatI	WGTACW	4 cut(s)	332, 597, 1136, 1768
TauI	GCSGC	2 cut(s)	2213, 2582
TfiI	GAWTC	9 cut(s)	316, 352, 731, 767, 895, 1553, 1652, 2120, 2353
Tru1I	TTAA	6 cut(s)	444, 1545, 1850, 2067, 2193, 2701
Tru9I	TTAA	6 cut(s)	444, 1545, 1850, 2067, 2193, 2701
TscAI	CASTG	8 cut(s)	44, 169, 328, 365, 397, 1441, 1630, 2387
TseFI	GTSAC	3 cut(s)	1659, 2378, 2621
Tsp45I	GTSAC	3 cut(s)	1659, 2378, 2621
TspGWI	ACGGA	2 cut(s)	929, 1697
TspRI	CASTG	8 cut(s)	44, 169, 328, 365, 397, 1441, 1630, 2387
Tth111I	GACNNNGTC	1 cut(s)	2619
VpaK11BI	GGWCC	3 cut(s)	1314, 2615, 2655
VspI	ATTAAT	1 cut(s)	2193
XapI	RAATTY	4 cut(s)	556, 850, 978, 2406
XbaI	TCTAGA	1 cut(s)	799
XceI	RCATGY	2 cut(s)	377, 1678
XhoI	CTCGAG	1 cut(s)	2666
XmiI	GTMKAC	1 cut(s)	5
XmnI	GAANNNNTTC	4 cut(s)	119, 237, 1380, 1745
XspI	CTAG	5 cut(s)	792, 800, 1616, 2085, 2202
ZraI	GACGTC	1 cut(s)	385
ZrmI	AGTACT	1 cut(s)	1770
Zsp2I	ATGCAT	1 cut(s)	2422

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.