Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000005

Orthogroup ID

OG0000937

Physical Location & Seq

Genomic Coordinates Reverse (-)

61390497 .. 61397644

Gene Structure 7148 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_5G00381380.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2286 bp

ATGTTGTGTAGTGAATGGGATCAACCTCCTGATGAAATGAGGCCACCTGGTTCAATTCCAATTAAGGACTATGCAATAGAAACACCCTTGAAGAAATCAGCATCATCTGTTGGAGATGATACCTCTCAGGAAGTGAAGATTGATATTCATCTTGACGGTGCAATTGCAGCCATAAATTTCGTTCTAAAGGATGAAGAAACTGGCACTTGGTATCAGTACAAAGGAAGAGATTTCAAAGTGCCTCTTGTGGGCTACCTGCAAGAATATGGCAATGTTGTTGCAGCAAAAAGGGAGTGGGGATATTGCCAGGCAGAATCATCAAACTCCCAAGATAAAAATAGCAGTAGTGAATCCAGAGGAACTAAAGAGGAAACTAGGTCTCTAGAAGGGTTCTATGAAGAACTCCCTATCGCAAAACAAATTGCTGTAGTGAATTCAGTAACTGTTTCTGTCAGGAAGTGCCCTGAGACAGCTAAGAATCTTCTATATTTGGAAACGGATTTACCTGATCATGTTGTTGTTCATTGGGGAGTTTGCAAAGATGATGCTAAAAGATGGGAAATTCCAGCAGCCCCACATCCGCCAGAAACAGTAGTGTTCAAGGATAAGGCTTTGCGAACTCGACTGCAGCAAAAAGAGGGAGCAAATGGACGTTGGGCATTGTTTACTTTAGATGAAGGAACCGCAGGATTTCTTTTTGTTTTCAAGCTAAGTGAAAGTACATGGTTGAAATGTAAGGGCAATGACTTCTACATCCCCCTTTCAAGCGCAAATAATTCACCTGCTGCAGCAAAAGAGGATCAATCTGAAGGTGCTAATGTAGATGAGAGAAGTGCAGAAGAAATAGATGAAAGTTCTTTTACTGAATATACCAATGGAATAATAAATGAAATAAGGACCCTGGTGAGTGGCATTTCCTCTGAGAAGAGTCGAAAGACAAAATCCAAAGAAGCACAAGAAAGCATTCTTCAAGAAATAGAAAAATTGGCTGCTGAAGCTTATAGTATCTTCAGAAGCACTGTTCCCACATTTACTGAGGAAGCTATTTCAGAAACTGAAGAATTACCACCCTCTGTGAAAATAACCTCAGGGACAGGCACGGGGTTTGAAATATTGTGCCAAGGTTTTAATTGGGAATCTCATAAATCTAGAAGATGGTACATGGAGCTCAAAGATAAAGCTGCTGAATTATCTTCGATAGGTTTCACTGTGATTTGGTTACCACCCCCTACAGATTCTGTGTCACCTGAAGGGTACATGCCGACGGATTTGTATAATCTGAACTCCAGATATGGCACTATGGATGAACTGAAGGAGACTGTGAAGGCATTCCATAAAGTTGGTATCAAAGTACTTGGAGATGTTGTTCTAAATCACCGCTGTGCGCAGTATCAGAATAGCAATGGTGTATGGAATATATTTGGTGGTCGTTTAAATTGGGATGATCGTGCAGTTGTTGCAGATGATCCACATTTTCAGGGTAGGGGCAACAAAAGTAGTGGAGATAGTTTTCATGCTGCACCAAACATTGATCATTCACAAGAGTTTGTGAGGAAGGATATCAAAGAATGGCTATGCTGGCTAAGGCAAGAAATTGGGTATGATGGATGGAGGCTTGATTTTGTTAGAGGATTTTGGGGTGGTTATGTTAAGGACTACATGGATGCTAGTGAGCCATACTTTGCTGTTGGTGAGTTTTGGGATTCCCTAAGTTATACGTATGGTGAGATGGATCACAATCAAGATGCACATAGGCAGAGAATTATTGATTGGATTAATGCTACTAGTGGAGCTGCTGGTGCTTTTGATGTAACAACAAAAGGCATCCTTCATGCTGTGTTGGAAAGATGTGAGTATTGGCGATTATCGGATCAGAAGGGAAAACCTCCTGGGGTTGTTGGATGGTGGCCATCTCGTGCTGTTACCTTCATAGAGAATCACGATACTGGTTCTACTCAGGGTCACTGGAGGTTTCCACATGACAAGGAAATTCAAGGATATGCATACATTCTGACTCATCCCGGAACACCTGCAGTCTTCTATGACCACATCTTCTCACATTACAAATCTGAAATTGCAGGTCTAATCTCTCTGAGAAACAGGAACACAATCAGCTGTCGGAGCAGAGTTAAAATTACCAAAGCTGAAAGAGATGTGTATGCCGCTATCATTGATGAAAAAGTAGCAATGAAGATCGGACCAGGTCACTATGAACCCCCAAATGGGCCTCAAAAATGGTCCAAAAGCCTTGAAGGAAAAGACTACAAGGTCTGGGAAGCATCTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000272 GO:0003674 GO:0003824 GO:0004553 GO:0004556 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0005976 GO:0005982 GO:0005983 GO:0005984 GO:0005985 GO:0005987 GO:0006073 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009251 GO:0009311 GO:0009313 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009987 GO:0016052 GO:0016160 GO:0016787 GO:0016798 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044042 GO:0044237 GO:0044238 GO:0044247 GO:0044248 GO:0044260 GO:0044262 GO:0044264 GO:0044275 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046352 GO:0071704 GO:1901575

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Alpha-amyl_C2 Alpha-amylase

Protein Analysis

761

Amino Acids

85.92

Weight (kDa)

5.41

Isoelectric Point (pI)

44.94

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ig_N_CWD1	PF23166	4 - 82	8.3e-20	Alpha-glucan water dikinase 1 Ig-like N-terminal domain
Ig_N_CWD1	PF23166	137 - 254	2.2e-34	Alpha-glucan water dikinase 1 Ig-like N-terminal domain
Alpha-amylase	PF00128	397 - 464	4.3e-13	Alpha amylase, catalytic domain
Alpha-amyl_C2	PF07821	701 - 759	9.5e-21	Alpha-amylase C-terminal beta-sheet domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000937)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G69830
fragaria_vesca	FvH4_4g31910 FvH4_4g31910
malus_domestica	MD13G1078600.v1.1 MD16G1078200.v1.1
prunus_persica	Prupe.1G268300_v2.0.a1 Prupe.1G268300_v2.0.a1
pyrus_communis	pycom13g06820 pycom16g06770
rosa_chinensis	RchiOBHm_Chr1g0315221 RchiOBHm_Chr1g0339091 RchiOBHm_Chr4g0440561 RchiOBHm_Chr5g0021421 RchiOBHm_Chr6g0272311
rosa_laevigata	RLG00000006168 RLG00000015039 RLG00000019642 RLG00000030692
rosa_multiflora	Rmu_co8071990.1_g000001 Rmu_sc0000829.1_g000011 Rmu_sc0002387.1_g000011 Rmu_sc0011312.1_g000002
rosa_roxburghii	Rroxscaffold_4G00298760 Rroxscaffold_5G00381380 Rroxscaffold_6G00426500 Rroxscaffold_7G00169140 Rroxscaffold_7G00214180
rosa_rugosa	Rorug01G0004200 Rorug04G0326700 Rorug05G0356300 Rorug05G0477400 Rorug05G0527600 Rorug07G0284000
rosa_samantha	Rh1AG270700 Rh1AG270800 Rh1AG346400 Rh1BG260100 Rh1CG254500 Rh2AG416800 Rh2CG403000 Rh3DG074600 Rh3DG292400 Rh4AG190200 Rh4AG246600 Rh4AG378400 Rh4BG035500 Rh4BG391100 Rh4CG405800 Rh4DG085900 Rh4DG385000 Rh5AG156300 Rh5AG268600 Rh5AG480700 Rh5BG155700 Rh5CG169500 Rh6AG024000 Rh6BG089200 Rh6DG025600 Rh7CG251800
rosa_wichuraiana	Rw1G024120 Rw1G030800 Rw4G032400 Rw4G032500 Rw7G034860

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	2 cut(s)	790, 2038
AasI	GACNNNNNNGTC	1 cut(s)	2267
Acc16I	TGCGCA	1 cut(s)	1386
Acc36I	ACCTGC	4 cut(s)	264, 790, 2038, 2069
AciI	CCGC	4 cut(s)	581, 684, 1378, 2163
AclWI	GGATC	5 cut(s)	27, 807, 1460, 1740, 1878
AcoI	YGGCCR	1 cut(s)	1907
AcsI	RAATTY	4 cut(s)	175, 433, 561, 1989
AcuI	CTGAAG	6 cut(s)	828, 994, 1014, 1077, 1269, 1331
AfaI	GTAC	5 cut(s)	218, 721, 1160, 1256, 1353
AfiI	CCNNNNNNNGG	3 cut(s)	248, 2222, 2223
AhlI	ACTAGT	1 cut(s)	1784
AjnI	CCWGG	5 cut(s)	46, 306, 900, 1888, 2200
AjuI	GAANNNNNNNTTGG	2 cut(s)	1753, 1785
AleI	CACNNNNGTG	1 cut(s)	1380
AloI	GAACNNNNNNTCC	2 cut(s)	1350, 1382
AluBI	AGCT	9 cut(s)	473, 709, 998, 1043, 1168, 1181, 1793, 2115, 2144
AluI	AGCT	9 cut(s)	473, 709, 998, 1043, 1168, 1181, 1793, 2115, 2144
Alw21I	GWGCWC	1 cut(s)	1170
Alw26I	GTCTC	3 cut(s)	384, 461, 1310
AlwI	GGATC	5 cut(s)	27, 807, 1460, 1740, 1878
AlwNI	CAGNNNCTG	3 cut(s)	443, 1055, 1238
AoxI	GGCC	3 cut(s)	41, 1907, 2225
ApoI	RAATTY	4 cut(s)	175, 433, 561, 1989
AseI	ATTAAT	1 cut(s)	1776
Asp700I	GAANNNNTTC	2 cut(s)	963, 1328
AspLEI	GCGC	2 cut(s)	770, 1387
AspS9I	GGNCC	4 cut(s)	897, 2198, 2225, 2238
AsuC2I	CCSGG	1 cut(s)	2022
AsuHPI	GGTGA	6 cut(s)	771, 916, 1236, 1367, 1703, 1736
AvaII	GGWCC	3 cut(s)	897, 2198, 2238
AxyI	CCTNAGG	1 cut(s)	1087
BaeGI	GKGCMC	1 cut(s)	464
BalI	TGGCCA	1 cut(s)	1909
BanII	GRGCYC	1 cut(s)	1170
BauI	CACGAG	1 cut(s)	1914
BbsI	GAAGAC	1 cut(s)	2029
Bbv12I	GWGCWC	1 cut(s)	1170
BccI	CCATC	7 cut(s)	549, 1149, 1598, 1602, 1723, 1896, 1918
BciT130I	CCWGG	5 cut(s)	48, 308, 902, 1890, 2202
BclI	TGATCA	2 cut(s)	508, 1531
BcnI	CCSGG	1 cut(s)	2022
BcoDI	GTCTC	3 cut(s)	384, 461, 1310
BcuI	ACTAGT	1 cut(s)	1784
BfaI	CTAG	5 cut(s)	375, 383, 1149, 1668, 1785
BfmI	CTRYAG	5 cut(s)	426, 626, 786, 1230, 2031
BfuAI	ACCTGC	4 cut(s)	264, 790, 2038, 2069
BmcAI	AGTACT	1 cut(s)	1353
Bme1390I	CCNGG	6 cut(s)	48, 308, 902, 1890, 2022, 2202
Bme18I	GGWCC	3 cut(s)	897, 2198, 2238
BmgT120I	GGNCC	4 cut(s)	897, 2198, 2225, 2238
BmiI	GGNNCC	2 cut(s)	682, 899
BmrFI	CCNGG	6 cut(s)	48, 308, 902, 1890, 2022, 2202
BmsI	GCATC	5 cut(s)	110, 535, 1654, 1735, 1833
BpiI	GAAGAC	1 cut(s)	2029
BpmI	CTGGAG	2 cut(s)	1270, 1987
Bpu10I	CCTNAGC	1 cut(s)	1583
BpuMI	CCSGG	1 cut(s)	2022
BsaAI	YACGTR	1 cut(s)	1719
BsaBI	GATNNNNATC	2 cut(s)	147, 1737
BsaI	GGTCTC	1 cut(s)	384
BsaJI	CCNNGG	3 cut(s)	900, 1120, 1889
BsaXI	ACNNNNNCTCC	2 cut(s)	1350, 1380
Bsc4I	CCNNNNNNNGG	3 cut(s)	248, 2222, 2223
Bse1I	ACTGG	3 cut(s)	205, 1951, 1970
Bse21I	CCTNAGG	1 cut(s)	1087
Bse3DI	GCAATG	4 cut(s)	277, 748, 1408, 2193
Bse8I	GATNNNNATC	2 cut(s)	147, 1737
BseBI	CCWGG	5 cut(s)	48, 308, 902, 1890, 2202
BseDI	CCNNGG	3 cut(s)	900, 1120, 1889
BseJI	GATNNNNATC	2 cut(s)	147, 1737
BseLI	CCNNNNNNNGG	3 cut(s)	248, 2222, 2223
BseMI	GCAATG	4 cut(s)	277, 748, 1408, 2193
BseMII	CTCAG	7 cut(s)	140, 456, 912, 1026, 1101, 1970, 2084
BseNI	ACTGG	3 cut(s)	205, 1951, 1970
BseSI	GKGCMC	1 cut(s)	464
BsgI	GTGCAG	3 cut(s)	855, 1470, 1503
BshFI	GGCC	3 cut(s)	43, 1909, 2227
BsiHKAI	GWGCWC	1 cut(s)	1170
BsiSI	CCGG	1 cut(s)	2022
BslFI	GGGAC	1 cut(s)	1105
BslI	CCNNNNNNNGG	3 cut(s)	248, 2222, 2223
BsmAI	GTCTC	3 cut(s)	384, 461, 1310
BsmFI	GGGAC	1 cut(s)	1105
BsmI	GAATGC	2 cut(s)	963, 1328
BsnI	GGCC	3 cut(s)	43, 1909, 2227
Bso31I	GGTCTC	1 cut(s)	384
Bsp1286I	GDGCHC	2 cut(s)	464, 1170
Bsp143I	GATC	9 cut(s)	19, 508, 799, 1444, 1465, 1531, 1732, 1870, 2193
BspACI	CCGC	4 cut(s)	581, 684, 1378, 2163
BspANI	GGCC	3 cut(s)	43, 1909, 2227
BspCNI	CTCAG	7 cut(s)	139, 457, 913, 1027, 1100, 1969, 2085
BspLI	GGNNCC	2 cut(s)	682, 899
BspMAI	CTGCAG	3 cut(s)	630, 790, 2035
BspMI	ACCTGC	4 cut(s)	264, 790, 2038, 2069
BspPI	GGATC	5 cut(s)	27, 807, 1460, 1740, 1878
BspTNI	GGTCTC	1 cut(s)	384
BsrDI	GCAATG	4 cut(s)	277, 748, 1408, 2193
BsrI	ACTGG	3 cut(s)	205, 1951, 1970
BssECI	CCNNGG	3 cut(s)	900, 1120, 1889
BssMI	GATC	9 cut(s)	19, 508, 799, 1444, 1465, 1531, 1732, 1870, 2193
BssSI	CACGAG	1 cut(s)	1914
BssT1I	CCWWGG	1 cut(s)	1120
Bst2BI	CACGAG	1 cut(s)	1914
Bst2UI	CCWGG	5 cut(s)	48, 308, 902, 1890, 2202
Bst4CI	ACNGT	6 cut(s)	158, 445, 592, 1021, 1210, 1321
Bst6I	CTCTTC	2 cut(s)	220, 920
BstAPI	GCANNNNNTGC	1 cut(s)	1457
BstBAI	YACGTR	1 cut(s)	1719
BstC8I	GCNNGC	1 cut(s)	1580
BstEII	GGTNACC	1 cut(s)	1218
BstHHI	GCGC	2 cut(s)	770, 1387
BstKTI	GATC	9 cut(s)	22, 511, 802, 1447, 1468, 1534, 1735, 1873, 2196
BstMAI	GTCTC	3 cut(s)	384, 461, 1310
BstMBI	GATC	9 cut(s)	19, 508, 799, 1444, 1465, 1531, 1732, 1870, 2193
BstMWI	GCNNNNNNNGC	6 cut(s)	167, 995, 1457, 1579, 1799, 2121
BstNI	CCWGG	5 cut(s)	48, 308, 902, 1890, 2202
BstNSI	RCATGY	1 cut(s)	1261
BstPI	GGTNACC	1 cut(s)	1218
BstSCI	CCNGG	6 cut(s)	46, 306, 900, 1888, 2020, 2200
BstSFI	CTRYAG	5 cut(s)	426, 626, 786, 1230, 2031
BstSLI	GKGCMC	1 cut(s)	464
BstSNI	TACGTA	1 cut(s)	1719
BstV2I	GAAGAC	1 cut(s)	2029
BstXI	CCANNNNNNTGG	1 cut(s)	1340
Bsu36I	CCTNAGG	1 cut(s)	1087
BsuRI	GGCC	3 cut(s)	43, 1909, 2227
BtsIMutI	CAGTG	3 cut(s)	1017, 1206, 1963
BveI	ACCTGC	4 cut(s)	264, 790, 2038, 2069
Cac8I	GCNNGC	1 cut(s)	1580
CaiI	CAGNNNCTG	3 cut(s)	443, 1055, 1238
CfoI	GCGC	2 cut(s)	770, 1387
Cfr13I	GGNCC	4 cut(s)	897, 2198, 2225, 2238
CsiI	ACCWGGT	2 cut(s)	46, 2200
Csp6I	GTAC	5 cut(s)	217, 720, 1159, 1255, 1352
CspCI	CAANNNNNGTGG	2 cut(s)	1480, 1515
CviAII	CATG	8 cut(s)	512, 723, 1162, 1258, 1514, 1660, 1832, 1979
CviQI	GTAC	5 cut(s)	217, 720, 1159, 1255, 1352
DpnI	GATC	9 cut(s)	21, 510, 801, 1446, 1467, 1533, 1734, 1872, 2195
DpnII	GATC	9 cut(s)	19, 508, 799, 1444, 1465, 1531, 1732, 1870, 2193
DraI	TTTAAA	1 cut(s)	1434
DrdI	GACNNNNNNGTC	1 cut(s)	2267
DseDI	GACNNNNNNGTC	1 cut(s)	2267
EaeI	YGGCCR	1 cut(s)	1907
Eam1104I	CTCTTC	2 cut(s)	220, 920
EarI	CTCTTC	2 cut(s)	220, 920
EciI	GGCGGA	1 cut(s)	570
Ecl136II	GAGCTC	1 cut(s)	1168
Eco105I	TACGTA	1 cut(s)	1719
Eco130I	CCWWGG	1 cut(s)	1120
Eco24I	GRGCYC	1 cut(s)	1170
Eco31I	GGTCTC	1 cut(s)	384
Eco32I	GATATC	1 cut(s)	1561
Eco47I	GGWCC	3 cut(s)	897, 2198, 2238
Eco53kI	GAGCTC	1 cut(s)	1168
Eco57I	CTGAAG	6 cut(s)	828, 994, 1014, 1077, 1269, 1331
Eco81I	CCTNAGG	1 cut(s)	1087
Eco91I	GGTNACC	1 cut(s)	1218
EcoICRI	GAGCTC	1 cut(s)	1168
EcoO109I	RGGNCCY	1 cut(s)	897
EcoO65I	GGTNACC	1 cut(s)	1218
EcoRI	GAATTC	1 cut(s)	433
EcoRII	CCWGG	5 cut(s)	46, 306, 900, 1888, 2200
EcoRV	GATATC	1 cut(s)	1561
EcoT14I	CCWWGG	1 cut(s)	1120
EcoT22I	ATGCAT	1 cut(s)	2005
EcoT38I	GRGCYC	1 cut(s)	1170
ErhI	CCWWGG	1 cut(s)	1120
FaeI	CATG	8 cut(s)	515, 726, 1165, 1261, 1517, 1663, 1835, 1982
FalI	AAGNNNNNCTT	4 cut(s)	228, 260, 1812, 1844
FaqI	GGGAC	1 cut(s)	1105
FatI	CATG	8 cut(s)	511, 722, 1161, 1257, 1513, 1659, 1831, 1978
FbaI	TGATCA	2 cut(s)	508, 1531
FriOI	GRGCYC	1 cut(s)	1170
FspBI	CTAG	5 cut(s)	375, 383, 1149, 1668, 1785
FspI	TGCGCA	1 cut(s)	1386
GlaI	GCGC	2 cut(s)	769, 1386
GsuI	CTGGAG	2 cut(s)	1270, 1987
HaeIII	GGCC	3 cut(s)	43, 1909, 2227
HapII	CCGG	1 cut(s)	2022
HhaI	GCGC	2 cut(s)	770, 1387
Hin1II	CATG	8 cut(s)	515, 726, 1165, 1261, 1517, 1663, 1835, 1982
Hin6I	GCGC	2 cut(s)	768, 1385
HinP1I	GCGC	2 cut(s)	768, 1385
HindIII	AAGCTT	1 cut(s)	996
HinfI	GANTC	9 cut(s)	314, 350, 478, 928, 1136, 1235, 1703, 1936, 2014
HpaII	CCGG	1 cut(s)	2022
HphI	GGTGA	6 cut(s)	771, 916, 1236, 1367, 1703, 1736
Hpy166II	GTNNAC	1 cut(s)	666
Hpy8I	GTNNAC	1 cut(s)	666
Hpy99I	CGWCG	1 cut(s)	1267
HpyCH4III	ACNGT	6 cut(s)	158, 445, 592, 1021, 1210, 1321
HpyCH4IV	ACGT	2 cut(s)	652, 1718
HpyF10VI	GCNNNNNNNGC	6 cut(s)	167, 995, 1457, 1579, 1799, 2121
HpySE526I	ACGT	2 cut(s)	652, 1718
Hsp92II	CATG	8 cut(s)	515, 726, 1165, 1261, 1517, 1663, 1835, 1982
HspAI	GCGC	2 cut(s)	768, 1385
Ksp22I	TGATCA	2 cut(s)	508, 1531
Kzo9I	GATC	9 cut(s)	19, 508, 799, 1444, 1465, 1531, 1732, 1870, 2193
LmnI	GCTCC	4 cut(s)	641, 1165, 1790, 2121
LweI	GCATC	5 cut(s)	110, 535, 1654, 1735, 1833
MabI	ACCWGGT	2 cut(s)	46, 2200
MaeI	CTAG	5 cut(s)	375, 383, 1149, 1668, 1785
MaeII	ACGT	2 cut(s)	652, 1718
MaeIII	GTNAC	7 cut(s)	439, 1218, 1242, 1810, 1921, 1961, 2204
MalI	GATC	9 cut(s)	21, 510, 801, 1446, 1467, 1533, 1734, 1872, 2195
MboI	GATC	9 cut(s)	19, 508, 799, 1444, 1465, 1531, 1732, 1870, 2193
MfeI	CAATTG	1 cut(s)	162
MhlI	GDGCHC	2 cut(s)	464, 1170
MlsI	TGGCCA	1 cut(s)	1909
MluNI	TGGCCA	1 cut(s)	1909
MlyI	GAGTC	2 cut(s)	937, 2008
MmeI	TCCRAC	4 cut(s)	91, 1821, 1879, 2099
Mox20I	TGGCCA	1 cut(s)	1909
Mph1103I	ATGCAT	1 cut(s)	2005
MroXI	GAANNNNTTC	2 cut(s)	963, 1328
MscI	TGGCCA	1 cut(s)	1909
MseI	TTAA	7 cut(s)	63, 1128, 1433, 1650, 1776, 2130, 2284
MslI	CAYNNNNRTG	1 cut(s)	1380
Msp20I	TGGCCA	1 cut(s)	1909
MspA1I	CMGCKG	2 cut(s)	1380, 2115
MspI	CCGG	1 cut(s)	2022
MspR9I	CCNGG	6 cut(s)	48, 308, 902, 1890, 2022, 2202
MunI	CAATTG	1 cut(s)	162
Mva1269I	GAATGC	2 cut(s)	963, 1328
MvaI	CCWGG	5 cut(s)	48, 308, 902, 1890, 2202
MwoI	GCNNNNNNNGC	6 cut(s)	167, 995, 1457, 1579, 1799, 2121
NciI	CCSGG	1 cut(s)	2022
NdeII	GATC	9 cut(s)	19, 508, 799, 1444, 1465, 1531, 1732, 1870, 2193
NlaIII	CATG	8 cut(s)	515, 726, 1165, 1261, 1517, 1663, 1835, 1982
NlaIV	GGNNCC	2 cut(s)	682, 899
NmuCI	GTSAC	3 cut(s)	1242, 1961, 2204
NsbI	TGCGCA	1 cut(s)	1386
NsiI	ATGCAT	1 cut(s)	2005
NspI	RCATGY	1 cut(s)	1261
OliI	CACNNNNGTG	1 cut(s)	1380
PaqCI	CACCTGC	2 cut(s)	790, 2038
PctI	GAATGC	2 cut(s)	963, 1328
PdmI	GAANNNNTTC	2 cut(s)	963, 1328
PfeI	GAWTC	7 cut(s)	314, 350, 478, 1136, 1235, 1703, 1936
PflFI	GACNNNGTC	1 cut(s)	2202
PfoI	TCCNGGA	1 cut(s)	2020
PleI	GAGTC	2 cut(s)	936, 2008
PpsI	GAGTC	2 cut(s)	936, 2008
Ppu21I	YACGTR	1 cut(s)	1719
PpuMI	RGGWCCY	1 cut(s)	897
PshBI	ATTAAT	1 cut(s)	1776
Psp124BI	GAGCTC	1 cut(s)	1170
Psp5II	RGGWCCY	1 cut(s)	897
Psp6I	CCWGG	5 cut(s)	46, 306, 900, 1888, 2200
PspEI	GGTNACC	1 cut(s)	1218
PspGI	CCWGG	5 cut(s)	46, 306, 900, 1888, 2200
PspN4I	GGNNCC	2 cut(s)	682, 899
PspPI	GGNCC	4 cut(s)	897, 2198, 2225, 2238
PspPPI	RGGWCCY	1 cut(s)	897
PstI	CTGCAG	3 cut(s)	630, 790, 2035
PstNI	CAGNNNCTG	3 cut(s)	443, 1055, 1238
PsyI	GACNNNGTC	1 cut(s)	2202
PvuII	CAGCTG	1 cut(s)	2115
RsaI	GTAC	5 cut(s)	218, 721, 1160, 1256, 1353
RsaNI	GTAC	5 cut(s)	217, 720, 1159, 1255, 1352
RseI	CAYNNNNRTG	1 cut(s)	1380
SacI	GAGCTC	1 cut(s)	1170
SaqAI	TTAA	7 cut(s)	63, 1128, 1433, 1650, 1776, 2130, 2284
Sau3AI	GATC	9 cut(s)	19, 508, 799, 1444, 1465, 1531, 1732, 1870, 2193
Sau96I	GGNCC	4 cut(s)	897, 2198, 2225, 2238
ScaI	AGTACT	1 cut(s)	1353
SchI	GAGTC	2 cut(s)	937, 2008
ScrFI	CCNGG	6 cut(s)	48, 308, 902, 1890, 2022, 2202
SduI	GDGCHC	2 cut(s)	464, 1170
SexAI	ACCWGGT	2 cut(s)	46, 2200
SfaNI	GCATC	5 cut(s)	110, 535, 1654, 1735, 1833
SfcI	CTRYAG	5 cut(s)	426, 626, 786, 1230, 2031
SinI	GGWCC	3 cut(s)	897, 2198, 2238
SmiMI	CAYNNNNRTG	1 cut(s)	1380
SnaBI	TACGTA	1 cut(s)	1719
SpeI	ACTAGT	1 cut(s)	1784
SsiI	CCGC	4 cut(s)	581, 684, 1378, 2163
SspI	AATATT	1 cut(s)	1113
SspMI	CTAG	5 cut(s)	375, 383, 1149, 1668, 1785
SstI	GAGCTC	1 cut(s)	1170
StyD4I	CCNGG	6 cut(s)	46, 306, 900, 1888, 2020, 2200
StyI	CCWWGG	1 cut(s)	1120
TaaI	ACNGT	6 cut(s)	158, 445, 592, 1021, 1210, 1321
TaiI	ACGT	2 cut(s)	655, 1721
TaqI	TCGA	3 cut(s)	622, 931, 1196
TatI	WGTACW	3 cut(s)	216, 719, 1351
TauI	GCSGC	1 cut(s)	2165
TfiI	GAWTC	7 cut(s)	314, 350, 478, 1136, 1235, 1703, 1936
Tru1I	TTAA	7 cut(s)	63, 1128, 1433, 1650, 1776, 2130, 2284
Tru9I	TTAA	7 cut(s)	63, 1128, 1433, 1650, 1776, 2130, 2284
TscAI	CASTG	3 cut(s)	1024, 1213, 1970
TseFI	GTSAC	3 cut(s)	1242, 1961, 2204
Tsp45I	GTSAC	3 cut(s)	1242, 1961, 2204
TspGWI	ACGGA	2 cut(s)	512, 1280
TspRI	CASTG	3 cut(s)	1024, 1213, 1970
Tth111I	GACNNNGTC	1 cut(s)	2202
VpaK11BI	GGWCC	3 cut(s)	897, 2198, 2238
VspI	ATTAAT	1 cut(s)	1776
XapI	RAATTY	4 cut(s)	175, 433, 561, 1989
XbaI	TCTAGA	2 cut(s)	382, 1148
XceI	RCATGY	1 cut(s)	1261
XmnI	GAANNNNTTC	2 cut(s)	963, 1328
XspI	CTAG	5 cut(s)	375, 383, 1149, 1668, 1785
ZrmI	AGTACT	1 cut(s)	1353
Zsp2I	ATGCAT	1 cut(s)	2005

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.