Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr4D

Orthogroup ID

OG0000937

Physical Location & Seq

Genomic Coordinates Reverse (-)

60219962 .. 60228415

Gene Structure 8454 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh4DG385000.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2457 bp

ATGTCGACGGTGAGCATTGAGCCTCTTCTCCACCACCACTGTCGCCGGGGAAACTCCCGGCACCGTTCAACGCCTCAGCTCATCCACCTCAGCTCCTCCTTGAGTGTCTTTCCGAAAAAGGTCGAGTTTCGTTGCCGGAGCTTGTGCAACTTCCGGCATCCCACTGCTCTCACTCTCAGAGCTTCTTCTACTGACACCGCCGTAGCTGCGACCACCGCGCCTTTCTTCAAGGAAACATTTCCTCTTGAGCTAACTGAACTGGTGGAGGGAAAGATCTTTGTGAGATTAGATCACGGGAAGAAGGCTCGGAATTGGATTCTCACTGTTGGTTGTACTCTTCCCGGAAAGTGGATTCTGCACTGGGGAGTTTCACATGTTGATGACGTCGGCAGTGAATGGGATCAACCTCCTGATGAAATGAGGCCGCCTGGTTCAATTCCAATTAAGGACTATGCAATAGAAACACCCTTGAAGAAATCAGCATCATCTGTTGGAGATGATACCTCTCAGGAAGTGAAGATTGATTTTCATCTTGACGGTGCAATTGCAGCCATAAATTTCGTTCTGAAGGATGAAGAAACTGGCACTTGGTATCAGTACAAAGGAAGAGATTTCAAAGTGCCTCTTGTGGGCTACCTGCAAGAATATGGCAATGTTGTTGGAGCAAAAAAGGGAGTGGGGATATTGCCAGGAGCTTTGGGGAAGTTATCCAACTTATTTTTCAAGGCAGAATCATCAAACTCCCAAGATAAAAATAGCAGTAGTGAATCCAGAGGAACTAAAGAGGAAACTAGGTCTCTAGAAGGGTTCTACGAAGAACTCCCTATCGCAAAACAAATTGCTGTAGTGAATTCAGTAACTGTTTCTGTCAGGAAGTGCCCTGAGACAGCTAAGAATCTTCTATATTTGGAAACGGATTTACCTGATCATGTTGTTGTTCATTGGGGAGTTTGCAAAGATGATGCTAAAAGATGGGAAATTCCAGCAGCCCCACATCCGCCAGAAACAGTAGTGTTCAAGGATAAGGCTTTGCGAACTCGACTGCAGCAAAAAGAGGGAGCAAATGGACGTTGGGCATTGTTTACTTTAGATGAAGGAACCGCAGGATTTCTTTTTGTTTTCAAGCTAAGTGAAAGTACATGGTTGAAATGTAAGGGCAATGACTTCTACATCCCCCTTTCAAGCGCAAATAATTCACCTGCTGCAGCAAAAGAGGATCAATCTGAAGGTGCTAATGTAGATGAGAGAAGTGCAGAAGAAATAGATGAAAGTTCTTTTTCTGAATATACCAATGGAATAATAAATGAAATAAGGACCCTGGTGAGTGGCATTTCCTCTGAGAAGAGTCGAAAGACAAAATCCAAAGAAGCGCAAGAAAGCATTCTTCAAGAAATAGAAAAATTGGCTGCTGAAGCCTATAGTATCTTCAGAACCACTGTTCCCACATTTACTGAGGAAACTATTTCAGAAACTGAAGAATTACCACCCTCTGTGAAAATAACCTCAGGGACAGGCACGGGGTTTGAAATATTGTGCCAAGGTTTTAATTGGGAATCTCATAAATCTGGAAGATGGTACATGGAGCTCAAAGATAAAGCTGCTGAATTATCTTCACTAGGTTTCACTGTGATTTGGTTACCACCCCCTACAGATTCTGTGTCACCTGAAGGGTACATGCCGACGGATTTGTATAATCTGAACTGCAGATATGGCACGATGGATGAACTGAAGGAGACTGTGAAGGCATTCCATAAAGTTGGTATCAAAGTACTTGGAGATGTTGTTCTAAATCACCGCTGTGCGCAGTATCAGAATAGCAATGGTGTATGGAATATATTTGGTGGTCGTTTAAATTGGGATGATCGTGCAGTTGTTGCAGATGATCCGCATTTTCAGGGTAGGGGCAACAAAAGTAGTGGAGATAATTTTCATGCTGCACCAAACATTGATCATTCACAAGAGTTTGTGAGGAAGGATATCAAAGAATGGTTATGCTGGCTAAGGCAAGAAATTGGGTATGATGGATGGAGGCTTGATTTTGTTCGAGGATTTTGGGGTGGTTATGTTAAGTACTACATGGATGCTAGCGAGCCATACTTTGCTGTTGGTGAGTTTTGGGATTCCCTAAGTTATACGTATGGTGAGATGGATCACAATCAAGATGCACATAGGCAGAGAATTATTGATTGGATTAATGCTACTAGTGGAGCCGCTGGTGCTTTTGATGTAACAACAAAAGGCATCCTTCATGCTGCGTTGGAAAGATGTGAGTATTGGCGATTATCGGATCAGAAGGGAAAACCTCCTGGGGTTGTTGGATGGTGGCCATCTCGTGCTGTTACCTTCATAGAGAATCACGATACTGGTTCTACTCAGGATTGGCCTTCAGTAGGAGCCCTTCCTAGCTGGACGATATCTGTCTTTTTTACATTGTGGATAGACCACTATACAATTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000272 GO:0003674 GO:0003824 GO:0004553 GO:0004556 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0005976 GO:0005982 GO:0005983 GO:0005984 GO:0005985 GO:0005987 GO:0006073 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009251 GO:0009311 GO:0009313 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009987 GO:0016052 GO:0016160 GO:0016787 GO:0016798 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044042 GO:0044237 GO:0044238 GO:0044247 GO:0044248 GO:0044260 GO:0044262 GO:0044264 GO:0044275 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046352 GO:0071704 GO:1901575

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Alpha-amyl_C2 Alpha-amylase

Protein Analysis

818

Amino Acids

91.88

Weight (kDa)

5.7

Isoelectric Point (pI)

41.37

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ig_N_CWD1	PF23166	88 - 209	7e-30	Alpha-glucan water dikinase 1 Ig-like N-terminal domain
Ig_N_CWD1	PF23166	276 - 393	2.5e-34	Alpha-glucan water dikinase 1 Ig-like N-terminal domain
Alpha-amylase	PF00128	536 - 603	6.8e-13	Alpha amylase, catalytic domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000937)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G69830
fragaria_vesca	FvH4_4g31910 FvH4_4g31910
malus_domestica	MD13G1078600.v1.1 MD16G1078200.v1.1
prunus_persica	Prupe.1G268300_v2.0.a1 Prupe.1G268300_v2.0.a1
pyrus_communis	pycom13g06820 pycom16g06770
rosa_chinensis	RchiOBHm_Chr1g0315221 RchiOBHm_Chr1g0339091 RchiOBHm_Chr4g0440561 RchiOBHm_Chr5g0021421 RchiOBHm_Chr6g0272311
rosa_laevigata	RLG00000006168 RLG00000015039 RLG00000019642 RLG00000030692
rosa_multiflora	Rmu_co8071990.1_g000001 Rmu_sc0000829.1_g000011 Rmu_sc0002387.1_g000011 Rmu_sc0011312.1_g000002
rosa_roxburghii	Rroxscaffold_4G00298760 Rroxscaffold_5G00381380 Rroxscaffold_6G00426500 Rroxscaffold_7G00169140 Rroxscaffold_7G00214180
rosa_rugosa	Rorug01G0004200 Rorug04G0326700 Rorug05G0356300 Rorug05G0477400 Rorug05G0527600 Rorug07G0284000
rosa_samantha	Rh1AG270700 Rh1AG270800 Rh1AG346400 Rh1BG260100 Rh1CG254500 Rh2AG416800 Rh2CG403000 Rh3DG074600 Rh3DG292400 Rh4AG190200 Rh4AG246600 Rh4AG378400 Rh4BG035500 Rh4BG391100 Rh4CG405800 Rh4DG085900 Rh4DG385000 Rh5AG156300 Rh5AG268600 Rh5AG480700 Rh5BG155700 Rh5CG169500 Rh6AG024000 Rh6BG089200 Rh6DG025600 Rh7CG251800
rosa_wichuraiana	Rw1G024120 Rw1G030800 Rw4G032400 Rw4G032500 Rw7G034860

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1207
AatII	GACGTC	1 cut(s)	387
Acc16I	TGCGCA	1 cut(s)	1803
Acc36I	ACCTGC	2 cut(s)	645, 1207
AccB1I	GGYRCC	1 cut(s)	60
AccI	GTMKAC	1 cut(s)	5
AccII	CGCG	1 cut(s)	218
AciI	CCGC	8 cut(s)	198, 216, 425, 998, 1101, 1795, 1886, 2211
AclWI	GGATC	5 cut(s)	408, 1224, 1877, 2157, 2295
AcoI	YGGCCR	1 cut(s)	2324
AcsI	RAATTY	3 cut(s)	556, 850, 978
AcuI	CTGAAG	8 cut(s)	587, 1245, 1411, 1431, 1494, 1686, 1748, 2370
AcyI	GRCGYC	1 cut(s)	384
AfaI	GTAC	7 cut(s)	334, 599, 1138, 1577, 1673, 1770, 2072
AfiI	CCNNNNNNNGG	3 cut(s)	348, 629, 2390
AflIII	ACRYGT	1 cut(s)	373
AhlI	ACTAGT	1 cut(s)	2201
AjnI	CCWGG	4 cut(s)	427, 688, 1317, 2305
AjuI	GAANNNNNNNTTGG	4 cut(s)	704, 736, 2170, 2202
AleI	CACNNNNGTG	1 cut(s)	1797
AloI	GAACNNNNNNTCC	4 cut(s)	1423, 1455, 1767, 1799
Alw21I	GWGCWC	1 cut(s)	1587
Alw26I	GTCTC	3 cut(s)	801, 878, 1727
AlwI	GGATC	5 cut(s)	408, 1224, 1877, 2157, 2295
AlwNI	CAGNNNCTG	3 cut(s)	860, 1472, 1655
AoxI	GGCC	3 cut(s)	422, 2324, 2381
ApoI	RAATTY	3 cut(s)	556, 850, 978
AseI	ATTAAT	1 cut(s)	2193
Asp700I	GAANNNNTTC	3 cut(s)	237, 1380, 1745
AspLEI	GCGC	4 cut(s)	220, 1187, 1372, 1804
AspS9I	GGNCC	1 cut(s)	1314
AsuC2I	CCSGG	3 cut(s)	47, 58, 342
AsuHPI	GGTGA	7 cut(s)	22, 1188, 1333, 1653, 1784, 2120, 2153
AsuNHI	GCTAGC	1 cut(s)	2084
AvaII	GGWCC	1 cut(s)	1314
AxyI	CCTNAGG	1 cut(s)	1504
BaeGI	GKGCMC	1 cut(s)	881
BalI	TGGCCA	1 cut(s)	2326
BanI	GGYRCC	1 cut(s)	60
BanII	GRGCYC	2 cut(s)	1587, 2398
BarI	GAAGNNNNNNTAC	2 cut(s)	2382, 2414
BauI	CACGAG	1 cut(s)	2331
Bbv12I	GWGCWC	1 cut(s)	1587
BbvCI	CCTCAGC	2 cut(s)	75, 89
BccI	CCATC	8 cut(s)	966, 1566, 1711, 2015, 2019, 2140, 2313, 2335
BceAI	ACGGC	1 cut(s)	185
BciT130I	CCWGG	4 cut(s)	429, 690, 1319, 2307
BclI	TGATCA	2 cut(s)	925, 1948
BcnI	CCSGG	3 cut(s)	47, 58, 342
BcoDI	GTCTC	3 cut(s)	801, 878, 1727
BcuI	ACTAGT	1 cut(s)	2201
BfaI	CTAG	6 cut(s)	792, 800, 1616, 2085, 2202, 2403
BfmI	CTRYAG	6 cut(s)	843, 1043, 1203, 1417, 1647, 1702
BfuAI	ACCTGC	2 cut(s)	645, 1207
BglII	AGATCT	1 cut(s)	273
BmcAI	AGTACT	2 cut(s)	1770, 2072
Bme1390I	CCNGG	7 cut(s)	47, 58, 342, 429, 690, 1319, 2307
Bme18I	GGWCC	1 cut(s)	1314
BmgT120I	GGNCC	1 cut(s)	1314
BmiI	GGNNCC	5 cut(s)	62, 1099, 1316, 2209, 2395
BmrFI	CCNGG	7 cut(s)	47, 58, 342, 429, 690, 1319, 2307
BmrI	ACTGGG	1 cut(s)	370
BmsI	GCATC	6 cut(s)	166, 491, 952, 2071, 2152, 2250
BmtI	GCTAGC	1 cut(s)	2088
BmuI	ACTGGG	1 cut(s)	370
Bpu10I	CCTNAGC	3 cut(s)	75, 89, 2000
BpuEI	CTTGAG	2 cut(s)	121, 266
BpuMI	CCSGG	3 cut(s)	47, 58, 342
BsaAI	YACGTR	1 cut(s)	2136
BsaBI	GATNNNNATC	2 cut(s)	528, 2154
BsaHI	GRCGYC	1 cut(s)	384
BsaI	GGTCTC	1 cut(s)	801
BsaJI	CCNNGG	4 cut(s)	46, 1317, 1537, 2306
BsaXI	ACNNNNNCTCC	4 cut(s)	77, 107, 1767, 1797
Bsc4I	CCNNNNNNNGG	3 cut(s)	348, 629, 2390
Bse1I	ACTGG	4 cut(s)	264, 365, 586, 2368
Bse21I	CCTNAGG	1 cut(s)	1504
Bse3DI	GCAATG	3 cut(s)	658, 1165, 1825
Bse8I	GATNNNNATC	2 cut(s)	528, 2154
BseBI	CCWGG	4 cut(s)	429, 690, 1319, 2307
BseDI	CCNNGG	4 cut(s)	46, 1317, 1537, 2306
BseJI	GATNNNNATC	2 cut(s)	528, 2154
BseLI	CCNNNNNNNGG	3 cut(s)	348, 629, 2390
BseMI	GCAATG	3 cut(s)	658, 1165, 1825
BseMII	CTCAG	9 cut(s)	89, 103, 190, 521, 873, 1329, 1443, 1518, 2387
BseNI	ACTGG	4 cut(s)	264, 365, 586, 2368
BseRI	GAGGAG	1 cut(s)	85
BseSI	GKGCMC	1 cut(s)	881
BsgI	GTGCAG	4 cut(s)	341, 1272, 1887, 1920
Bsh1236I	CGCG	1 cut(s)	218
BshFI	GGCC	3 cut(s)	424, 2326, 2383
BshNI	GGYRCC	1 cut(s)	60
BsiHKAI	GWGCWC	1 cut(s)	1587
BsiSI	CCGG	5 cut(s)	46, 58, 136, 154, 342
BslFI	GGGAC	1 cut(s)	1522
BslI	CCNNNNNNNGG	3 cut(s)	348, 629, 2390
BsmAI	GTCTC	3 cut(s)	801, 878, 1727
BsmFI	GGGAC	1 cut(s)	1522
BsmI	GAATGC	2 cut(s)	1380, 1745
BsnI	GGCC	3 cut(s)	424, 2326, 2383
Bso31I	GGTCTC	1 cut(s)	801
Bsp1286I	GDGCHC	3 cut(s)	881, 1587, 2398
BspACI	CCGC	8 cut(s)	198, 216, 425, 998, 1101, 1795, 1886, 2211
BspANI	GGCC	3 cut(s)	424, 2326, 2383
BspCNI	CTCAG	9 cut(s)	88, 102, 189, 520, 874, 1330, 1444, 1517, 2386
BspFNI	CGCG	1 cut(s)	218
BspLI	GGNNCC	5 cut(s)	62, 1099, 1316, 2209, 2395
BspMAI	CTGCAG	3 cut(s)	1047, 1207, 1706
BspMI	ACCTGC	2 cut(s)	645, 1207
BspOI	GCTAGC	1 cut(s)	2088
BspPI	GGATC	5 cut(s)	408, 1224, 1877, 2157, 2295
BspT107I	GGYRCC	1 cut(s)	60
BspTNI	GGTCTC	1 cut(s)	801
BsrDI	GCAATG	3 cut(s)	658, 1165, 1825
BsrI	ACTGG	4 cut(s)	264, 365, 586, 2368
BssECI	CCNNGG	4 cut(s)	46, 1317, 1537, 2306
BssNI	GRCGYC	1 cut(s)	384
BssSI	CACGAG	1 cut(s)	2331
BssT1I	CCWWGG	1 cut(s)	1537
Bst2BI	CACGAG	1 cut(s)	2331
Bst2UI	CCWGG	4 cut(s)	429, 690, 1319, 2307
Bst6I	CTCTTC	4 cut(s)	30, 342, 601, 1337
BstACI	GRCGYC	1 cut(s)	384
BstAPI	GCANNNNNTGC	1 cut(s)	1874
BstBAI	YACGTR	1 cut(s)	2136
BstC8I	GCNNGC	3 cut(s)	1997, 2086, 2090
BstEII	GGTNACC	1 cut(s)	1635
BstENI	CCTNNNNNAGG	1 cut(s)	2388
BstFNI	CGCG	1 cut(s)	218
BstHHI	GCGC	4 cut(s)	220, 1187, 1372, 1804
BstMAI	GTCTC	3 cut(s)	801, 878, 1727
BstMWI	GCNNNNNNNGC	7 cut(s)	206, 215, 548, 1412, 1710, 1874, 2216
BstNI	CCWGG	4 cut(s)	429, 690, 1319, 2307
BstNSI	RCATGY	2 cut(s)	377, 1678
BstPI	GGTNACC	1 cut(s)	1635
BstSCI	CCNGG	7 cut(s)	45, 56, 340, 427, 688, 1317, 2305
BstSFI	CTRYAG	6 cut(s)	843, 1043, 1203, 1417, 1647, 1702
BstSLI	GKGCMC	1 cut(s)	881
BstSNI	TACGTA	1 cut(s)	2136
BstUI	CGCG	1 cut(s)	218
BstX2I	RGATCY	1 cut(s)	273
BstXI	CCANNNNNNTGG	1 cut(s)	1757
BstYI	RGATCY	1 cut(s)	273
Bsu36I	CCTNAGG	1 cut(s)	1504
BsuRI	GGCC	3 cut(s)	424, 2326, 2383
BtsI	GCAGTG	2 cut(s)	162, 397
BtsIMutI	CAGTG	7 cut(s)	37, 162, 321, 358, 397, 1434, 1623
BveI	ACCTGC	2 cut(s)	645, 1207
Cac8I	GCNNGC	3 cut(s)	1997, 2086, 2090
CaiI	CAGNNNCTG	3 cut(s)	860, 1472, 1655
CfoI	GCGC	4 cut(s)	220, 1187, 1372, 1804
Cfr13I	GGNCC	1 cut(s)	1314
Csp6I	GTAC	7 cut(s)	333, 598, 1137, 1576, 1672, 1769, 2071
CspCI	CAANNNNNGTGG	2 cut(s)	1897, 1932
CviAII	CATG	8 cut(s)	374, 929, 1140, 1579, 1675, 1931, 2077, 2249
CviQI	GTAC	7 cut(s)	333, 598, 1137, 1576, 1672, 1769, 2071
DraI	TTTAAA	1 cut(s)	1851
EaeI	YGGCCR	1 cut(s)	2324
Eam1104I	CTCTTC	4 cut(s)	30, 342, 601, 1337
EarI	CTCTTC	4 cut(s)	30, 342, 601, 1337
EciI	GGCGGA	1 cut(s)	987
Ecl136II	GAGCTC	1 cut(s)	1585
Eco105I	TACGTA	1 cut(s)	2136
Eco130I	CCWWGG	1 cut(s)	1537
Eco24I	GRGCYC	2 cut(s)	1587, 2398
Eco31I	GGTCTC	1 cut(s)	801
Eco32I	GATATC	2 cut(s)	1978, 2415
Eco47I	GGWCC	1 cut(s)	1314
Eco53kI	GAGCTC	1 cut(s)	1585
Eco57I	CTGAAG	8 cut(s)	587, 1245, 1411, 1431, 1494, 1686, 1748, 2370
Eco81I	CCTNAGG	1 cut(s)	1504
Eco91I	GGTNACC	1 cut(s)	1635
EcoICRI	GAGCTC	1 cut(s)	1585
EcoNI	CCTNNNNNAGG	1 cut(s)	2388
EcoO109I	RGGNCCY	1 cut(s)	1314
EcoO65I	GGTNACC	1 cut(s)	1635
EcoRI	GAATTC	1 cut(s)	850
EcoRII	CCWGG	4 cut(s)	427, 688, 1317, 2305
EcoRV	GATATC	2 cut(s)	1978, 2415
EcoT14I	CCWWGG	1 cut(s)	1537
EcoT38I	GRGCYC	2 cut(s)	1587, 2398
ErhI	CCWWGG	1 cut(s)	1537
FaeI	CATG	8 cut(s)	377, 932, 1143, 1582, 1678, 1934, 2080, 2252
FalI	AAGNNNNNCTT	4 cut(s)	609, 641, 2229, 2261
FaqI	GGGAC	1 cut(s)	1522
FatI	CATG	8 cut(s)	373, 928, 1139, 1578, 1674, 1930, 2076, 2248
FbaI	TGATCA	2 cut(s)	925, 1948
FblI	GTMKAC	1 cut(s)	5
FriOI	GRGCYC	2 cut(s)	1587, 2398
FspBI	CTAG	6 cut(s)	792, 800, 1616, 2085, 2202, 2403
FspI	TGCGCA	1 cut(s)	1803
GlaI	GCGC	4 cut(s)	219, 1186, 1371, 1803
HaeIII	GGCC	3 cut(s)	424, 2326, 2383
HapII	CCGG	5 cut(s)	46, 58, 136, 154, 342
HhaI	GCGC	4 cut(s)	220, 1187, 1372, 1804
Hin1I	GRCGYC	1 cut(s)	384
Hin1II	CATG	8 cut(s)	377, 932, 1143, 1582, 1678, 1934, 2080, 2252
Hin6I	GCGC	4 cut(s)	218, 1185, 1370, 1802
HinP1I	GCGC	4 cut(s)	218, 1185, 1370, 1802
HincII	GTYRAC	1 cut(s)	6
HindII	GTYRAC	1 cut(s)	6
HpaII	CCGG	5 cut(s)	46, 58, 136, 154, 342
HphI	GGTGA	7 cut(s)	22, 1188, 1333, 1653, 1784, 2120, 2153
Hpy166II	GTNNAC	2 cut(s)	6, 1083
Hpy8I	GTNNAC	2 cut(s)	6, 1083
Hpy99I	CGWCG	3 cut(s)	10, 389, 1684
HpyCH4IV	ACGT	3 cut(s)	384, 1069, 2135
HpyF10VI	GCNNNNNNNGC	7 cut(s)	206, 215, 548, 1412, 1710, 1874, 2216
HpySE526I	ACGT	3 cut(s)	384, 1069, 2135
Hsp92I	GRCGYC	1 cut(s)	384
Hsp92II	CATG	8 cut(s)	377, 932, 1143, 1582, 1678, 1934, 2080, 2252
HspAI	GCGC	4 cut(s)	218, 1185, 1370, 1802
Ksp22I	TGATCA	2 cut(s)	925, 1948
LmnI	GCTCC	8 cut(s)	98, 138, 662, 692, 1058, 1582, 2207, 2393
LweI	GCATC	6 cut(s)	166, 491, 952, 2071, 2152, 2250
MaeI	CTAG	6 cut(s)	792, 800, 1616, 2085, 2202, 2403
MaeII	ACGT	3 cut(s)	384, 1069, 2135
MaeIII	GTNAC	5 cut(s)	856, 1635, 1659, 2227, 2338
MfeI	CAATTG	1 cut(s)	543
MflI	RGATCY	1 cut(s)	273
MhlI	GDGCHC	3 cut(s)	881, 1587, 2398
MlsI	TGGCCA	1 cut(s)	2326
MluNI	TGGCCA	1 cut(s)	2326
MlyI	GAGTC	1 cut(s)	1354
MmeI	TCCRAC	5 cut(s)	472, 640, 735, 2238, 2296
Mox20I	TGGCCA	1 cut(s)	2326
MroXI	GAANNNNTTC	3 cut(s)	237, 1380, 1745
MscI	TGGCCA	1 cut(s)	2326
MseI	TTAA	6 cut(s)	444, 1545, 1850, 2067, 2193, 2455
MslI	CAYNNNNRTG	2 cut(s)	378, 1797
Msp20I	TGGCCA	1 cut(s)	2326
MspA1I	CMGCKG	2 cut(s)	1797, 2213
MspI	CCGG	5 cut(s)	46, 58, 136, 154, 342
MspR9I	CCNGG	7 cut(s)	47, 58, 342, 429, 690, 1319, 2307
MunI	CAATTG	1 cut(s)	543
Mva1269I	GAATGC	2 cut(s)	1380, 1745
MvaI	CCWGG	4 cut(s)	429, 690, 1319, 2307
MvnI	CGCG	1 cut(s)	218
MwoI	GCNNNNNNNGC	7 cut(s)	206, 215, 548, 1412, 1710, 1874, 2216
NciI	CCSGG	3 cut(s)	47, 58, 342
NheI	GCTAGC	1 cut(s)	2084
NlaIII	CATG	8 cut(s)	377, 932, 1143, 1582, 1678, 1934, 2080, 2252
NlaIV	GGNNCC	5 cut(s)	62, 1099, 1316, 2209, 2395
NmuCI	GTSAC	1 cut(s)	1659
NsbI	TGCGCA	1 cut(s)	1803
NspI	RCATGY	2 cut(s)	377, 1678
OliI	CACNNNNGTG	1 cut(s)	1797
PaqCI	CACCTGC	1 cut(s)	1207
PciI	ACATGT	1 cut(s)	373
PctI	GAATGC	2 cut(s)	1380, 1745
PdmI	GAANNNNTTC	3 cut(s)	237, 1380, 1745
PfeI	GAWTC	9 cut(s)	316, 352, 731, 767, 895, 1553, 1652, 2120, 2353
PfoI	TCCNGGA	1 cut(s)	340
PleI	GAGTC	1 cut(s)	1353
PpsI	GAGTC	1 cut(s)	1353
Ppu21I	YACGTR	1 cut(s)	2136
PpuMI	RGGWCCY	1 cut(s)	1314
PscI	ACATGT	1 cut(s)	373
PshBI	ATTAAT	1 cut(s)	2193
Psp124BI	GAGCTC	1 cut(s)	1587
Psp5II	RGGWCCY	1 cut(s)	1314
Psp6I	CCWGG	4 cut(s)	427, 688, 1317, 2305
PspEI	GGTNACC	1 cut(s)	1635
PspGI	CCWGG	4 cut(s)	427, 688, 1317, 2305
PspN4I	GGNNCC	5 cut(s)	62, 1099, 1316, 2209, 2395
PspPI	GGNCC	1 cut(s)	1314
PspPPI	RGGWCCY	1 cut(s)	1314
PstI	CTGCAG	3 cut(s)	1047, 1207, 1706
PstNI	CAGNNNCTG	3 cut(s)	860, 1472, 1655
PsuI	RGATCY	1 cut(s)	273
RsaI	GTAC	7 cut(s)	334, 599, 1138, 1577, 1673, 1770, 2072
RsaNI	GTAC	7 cut(s)	333, 598, 1137, 1576, 1672, 1769, 2071
RseI	CAYNNNNRTG	2 cut(s)	378, 1797
SacI	GAGCTC	1 cut(s)	1587
SalI	GTCGAC	1 cut(s)	4
SaqAI	TTAA	6 cut(s)	444, 1545, 1850, 2067, 2193, 2455
Sau96I	GGNCC	1 cut(s)	1314
ScaI	AGTACT	2 cut(s)	1770, 2072
SchI	GAGTC	1 cut(s)	1354
ScrFI	CCNGG	7 cut(s)	47, 58, 342, 429, 690, 1319, 2307
SduI	GDGCHC	3 cut(s)	881, 1587, 2398
SfaNI	GCATC	6 cut(s)	166, 491, 952, 2071, 2152, 2250
SfcI	CTRYAG	6 cut(s)	843, 1043, 1203, 1417, 1647, 1702
SinI	GGWCC	1 cut(s)	1314
SmiMI	CAYNNNNRTG	2 cut(s)	378, 1797
SmlI	CTYRAG	2 cut(s)	100, 245
SmoI	CTYRAG	2 cut(s)	100, 245
SnaBI	TACGTA	1 cut(s)	2136
SpeI	ACTAGT	1 cut(s)	2201
SsiI	CCGC	8 cut(s)	198, 216, 425, 998, 1101, 1795, 1886, 2211
SspI	AATATT	1 cut(s)	1530
SspMI	CTAG	6 cut(s)	792, 800, 1616, 2085, 2202, 2403
SstI	GAGCTC	1 cut(s)	1587
StyD4I	CCNGG	7 cut(s)	45, 56, 340, 427, 688, 1317, 2305
StyI	CCWWGG	1 cut(s)	1537
TaiI	ACGT	3 cut(s)	387, 1072, 2138
TaqI	TCGA	5 cut(s)	5, 123, 1039, 1348, 2044
TatI	WGTACW	5 cut(s)	332, 597, 1136, 1768, 2070
TauI	GCSGC	2 cut(s)	427, 2213
TfiI	GAWTC	9 cut(s)	316, 352, 731, 767, 895, 1553, 1652, 2120, 2353
Tru1I	TTAA	6 cut(s)	444, 1545, 1850, 2067, 2193, 2455
Tru9I	TTAA	6 cut(s)	444, 1545, 1850, 2067, 2193, 2455
TscAI	CASTG	7 cut(s)	44, 169, 328, 365, 397, 1441, 1630
TseFI	GTSAC	1 cut(s)	1659
Tsp45I	GTSAC	1 cut(s)	1659
TspGWI	ACGGA	2 cut(s)	929, 1697
TspRI	CASTG	7 cut(s)	44, 169, 328, 365, 397, 1441, 1630
VpaK11BI	GGWCC	1 cut(s)	1314
VspI	ATTAAT	1 cut(s)	2193
XagI	CCTNNNNNAGG	1 cut(s)	2388
XapI	RAATTY	3 cut(s)	556, 850, 978
XbaI	TCTAGA	1 cut(s)	799
XceI	RCATGY	2 cut(s)	377, 1678
XmiI	GTMKAC	1 cut(s)	5
XmnI	GAANNNNTTC	3 cut(s)	237, 1380, 1745
XspI	CTAG	6 cut(s)	792, 800, 1616, 2085, 2202, 2403
ZraI	GACGTC	1 cut(s)	385
ZrmI	AGTACT	2 cut(s)	1770, 2072

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.