Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr4C

Orthogroup ID

OG0000937

Physical Location & Seq

Genomic Coordinates Reverse (-)

65289795 .. 65298627

Gene Structure 8833 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh4CG405800.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2070 bp

ATGTCGACGGTGAGCATTGAGCCTCTTCTCCACCACCACTGTCGCCGGGGAAACTCGCGGCACCGTTCAACGCCGAAGCTCATCAACCTCAGCTCCTCCCTGAGTGCCTTTCCGAAAAAGGTGGAGTTTCGTGGCCGGAGCTTCTGCAACTTCCGGCATCCCACTGCTCTCACTCTCAGAGCTTCTTCTACTGACACCGCCGTAGCTGCGACCACCGCGCCTTTCTTCAAGGAAACATTTCCTCTTGAGCGAACTGAACTGGTGGAGGGAAAGATCTCTGTGAGATTAGATCACGGGAAGAATGCTCGGAATTGGATTCTTACTGTTGGTTGTACTCTTCCCGGAAAGTGGATTCTGCACTGGGGAGTTTCACAGGTTGATGACGTCGGCAGTGAATGGGATCAACCTCCTGATGAAATGAGGCCACCTGGTTCAATTCCAATTAAGGACTATGCAATAGAAACACCCTTGAACAAATCAGCATCATCTGTTGGAGATGATACCTCTCAGGAAGTGAAGATTGATTTTCATCTTGACGGTGCAATTGCAGCCATAAATTTCGTTCTAAAGGATGAAGAAACTGGCACTTGGTATCAGTACAAAGGAAGAGATTTCAAAGTGCCTCTTGTGGGCTACCTGCAAGAATATGGCAATGTTGTTGGAGCAAAAAAGGGAGTGGGGATATTGCCAGGAGCTTTGGGGAAGTTATCCAACTTATTTTTCAAGGCAGAATCATCAAACTCCCAAGATAAAAATAGCAGTAGTGAATCCAGAGGAACTAAAGAGGAAACTAGGTCTCTAGAAGGGTTCTACGAAGAACTCCCTATCGCAAAACAAATTGCTGTAGTGAATTCAGTAACTGTTTCTGTCAGGAAGTGCCCTGAGACAGCTAAGAATCTTCTATATTTGGAAACGGATTTACCTGATCATGTTGTTGTTCATTGGGGAGTTTGCAAAGATGATGCTAAAAGATGGGAAATTCCAGCAGCCCCACATCCGCCAGAAACAGTAGTGTTCAAGGATAAGGCTTTGCGAACTCGACTGCAGCAAAAAGAGGGAGCAAATGGACGTTGGGCATTGTTTACTTTAGATGAAGGAACCGCAGGATTTCTTTTTGTTTTCAAGCTAAGTGAAAGTACATGGTTGAAATGTAAGGGCAATGACTTCTACATCCCCCTTTCAAGCGCAAATAATTCACCTGCTGCAGCAAAAGAGGATCAATCTGAAGGTGCTAATGTCGATGAGAGAAGTGCAGAAGAAATAGATGAAAGTTCTTTTACTGAATATACCAATGGAATAATAAATGAAATAAGGACCTTGGTGAGTGGCATTTCCTCTGAGAAGAGTCGAAAGACAAAATCCAAAGAAGCACAAGAAAGCATTCTTCAAGAAATAGAAAAATTGGCTGCTGAAGCCTATAGTATATTCAGAAGCACTGTTCCCACATTTACTGAGGAAGCTATTTCAGAAACTGAAGAATTACCCCCCTCTGTGAAAATAACCTCAGGGACAGGCACGGGGTTTGAAATATTGTGCCAAGGTTTTAATTGGGAATCTCATAAATCTGGAAGATGGTACATGGAGCTCAAAGATAAAGCTGCTGAACTATCTTCACTAGGTTTCACTGTGATTTGGTTACCACCCCCTACAGATTCTGTGTCACCTGAAGGGTACATGCCGACAGATTTGTATAATCTGAACTCCAGATATGGCACTATGGATGAACTGAAGGAGACTGTGAAGGCATTCCATAAAGTTGGTATCAAAGTACTTGGAGATGTTGTTCTAAATCACCGCTGTGCGCAGTATAAGAATAGCAATGGTGTATGGAATATATTTGGTGGTCGTTTAAATTGGGATGATCGTGCAGTTGTTGCAGATGATCCGCATTTTCAGGGTAGGGGCAACAAAAGTAGTGGAGATAGTTTTCATGCTGCACCAAACATTGATCATTCACAAGAGTTTGTGAGGAAGGATATCAAAGAATGGTTATGCTGGCTAAGGTTAAAAGTCTGGACTATTTACTCAATTCTTGGGATTCTATCCTTATTGTTTGCAAGTTTAACTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000272 GO:0003674 GO:0003824 GO:0004553 GO:0004556 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0005976 GO:0005982 GO:0005983 GO:0005984 GO:0005985 GO:0005987 GO:0006073 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009251 GO:0009311 GO:0009313 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009987 GO:0016052 GO:0016160 GO:0016787 GO:0016798 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044042 GO:0044237 GO:0044238 GO:0044247 GO:0044248 GO:0044260 GO:0044262 GO:0044264 GO:0044275 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046352 GO:0071704 GO:1901575

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Alpha-amyl_C2 Alpha-amylase

Protein Analysis

689

Amino Acids

76.78

Weight (kDa)

6.01

Isoelectric Point (pI)

44.01

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ig_N_CWD1	PF23166	87 - 209	1.7e-30	Alpha-glucan water dikinase 1 Ig-like N-terminal domain
Ig_N_CWD1	PF23166	276 - 393	1.9e-34	Alpha-glucan water dikinase 1 Ig-like N-terminal domain
Alpha-amylase	PF00128	536 - 603	2e-13	Alpha amylase, catalytic domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000937)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G69830
fragaria_vesca	FvH4_4g31910 FvH4_4g31910
malus_domestica	MD13G1078600.v1.1 MD16G1078200.v1.1
prunus_persica	Prupe.1G268300_v2.0.a1 Prupe.1G268300_v2.0.a1
pyrus_communis	pycom13g06820 pycom16g06770
rosa_chinensis	RchiOBHm_Chr1g0315221 RchiOBHm_Chr1g0339091 RchiOBHm_Chr4g0440561 RchiOBHm_Chr5g0021421 RchiOBHm_Chr6g0272311
rosa_laevigata	RLG00000006168 RLG00000015039 RLG00000019642 RLG00000030692
rosa_multiflora	Rmu_co8071990.1_g000001 Rmu_sc0000829.1_g000011 Rmu_sc0002387.1_g000011 Rmu_sc0011312.1_g000002
rosa_roxburghii	Rroxscaffold_4G00298760 Rroxscaffold_5G00381380 Rroxscaffold_6G00426500 Rroxscaffold_7G00169140 Rroxscaffold_7G00214180
rosa_rugosa	Rorug01G0004200 Rorug04G0326700 Rorug05G0356300 Rorug05G0477400 Rorug05G0527600 Rorug07G0284000
rosa_samantha	Rh1AG270700 Rh1AG270800 Rh1AG346400 Rh1BG260100 Rh1CG254500 Rh2AG416800 Rh2CG403000 Rh3DG074600 Rh3DG292400 Rh4AG190200 Rh4AG246600 Rh4AG378400 Rh4BG035500 Rh4BG391100 Rh4CG405800 Rh4DG085900 Rh4DG385000 Rh5AG156300 Rh5AG268600 Rh5AG480700 Rh5BG155700 Rh5CG169500 Rh6AG024000 Rh6BG089200 Rh6DG025600 Rh7CG251800
rosa_wichuraiana	Rw1G024120 Rw1G030800 Rw4G032400 Rw4G032500 Rw7G034860

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1207
AatII	GACGTC	1 cut(s)	387
Acc16I	TGCGCA	1 cut(s)	1803
Acc36I	ACCTGC	2 cut(s)	645, 1207
AccB1I	GGYRCC	1 cut(s)	60
AccI	GTMKAC	1 cut(s)	5
AccII	CGCG	2 cut(s)	58, 218
AciI	CCGC	7 cut(s)	58, 198, 216, 998, 1101, 1795, 1886
AclWI	GGATC	3 cut(s)	408, 1224, 1877
AcoI	YGGCCR	1 cut(s)	133
AcsI	RAATTY	3 cut(s)	556, 850, 978
AcuI	CTGAAG	5 cut(s)	1245, 1431, 1494, 1686, 1748
AcyI	GRCGYC	1 cut(s)	384
AfaI	GTAC	6 cut(s)	334, 599, 1138, 1577, 1673, 1770
AfiI	CCNNNNNNNGG	2 cut(s)	348, 629
AjnI	CCWGG	2 cut(s)	427, 688
AjuI	GAANNNNNNNTTGG	2 cut(s)	704, 736
AleI	CACNNNNGTG	1 cut(s)	1797
AloI	GAACNNNNNNTCC	2 cut(s)	1767, 1799
Alw21I	GWGCWC	1 cut(s)	1587
Alw26I	GTCTC	3 cut(s)	801, 878, 1727
AlwI	GGATC	3 cut(s)	408, 1224, 1877
AlwNI	CAGNNNCTG	3 cut(s)	860, 1472, 1655
AoxI	GGCC	2 cut(s)	133, 422
ApeKI	GCWGC	9 cut(s)	206, 548, 986, 1045, 1202, 1205, 1406, 1598, 1934
ApoI	RAATTY	3 cut(s)	556, 850, 978
Asp700I	GAANNNNTTC	3 cut(s)	237, 1380, 1745
AspLEI	GCGC	3 cut(s)	220, 1187, 1804
AspS9I	GGNCC	1 cut(s)	1314
AsuC2I	CCSGG	2 cut(s)	47, 342
AsuHPI	GGTGA	5 cut(s)	22, 1188, 1333, 1653, 1784
AvaII	GGWCC	1 cut(s)	1314
AxyI	CCTNAGG	1 cut(s)	1504
BaeGI	GKGCMC	1 cut(s)	881
BanI	GGYRCC	1 cut(s)	60
BanII	GRGCYC	1 cut(s)	1587
Bbv12I	GWGCWC	1 cut(s)	1587
BbvCI	CCTCAGC	1 cut(s)	89
BbvI	GCAGC	9 cut(s)	193, 560, 998, 1057, 1189, 1217, 1393, 1585, 1921
BccI	CCATC	2 cut(s)	966, 1566
BceAI	ACGGC	1 cut(s)	185
BciT130I	CCWGG	2 cut(s)	429, 690
BclI	TGATCA	2 cut(s)	925, 1948
BcnI	CCSGG	2 cut(s)	47, 342
BcoDI	GTCTC	3 cut(s)	801, 878, 1727
BfaI	CTAG	3 cut(s)	792, 800, 1616
BfmI	CTRYAG	5 cut(s)	843, 1043, 1203, 1417, 1647
BfuAI	ACCTGC	2 cut(s)	645, 1207
BglII	AGATCT	1 cut(s)	273
BmcAI	AGTACT	1 cut(s)	1770
Bme1390I	CCNGG	4 cut(s)	47, 342, 429, 690
Bme18I	GGWCC	1 cut(s)	1314
BmgT120I	GGNCC	1 cut(s)	1314
BmiI	GGNNCC	2 cut(s)	62, 1099
BmrFI	CCNGG	4 cut(s)	47, 342, 429, 690
BmrI	ACTGGG	1 cut(s)	370
BmsI	GCATC	3 cut(s)	166, 491, 952
BmuI	ACTGGG	1 cut(s)	370
BpmI	CTGGAG	1 cut(s)	1687
Bpu10I	CCTNAGC	2 cut(s)	89, 2000
BpuEI	CTTGAG	1 cut(s)	266
BpuMI	CCSGG	2 cut(s)	47, 342
BsaBI	GATNNNNATC	1 cut(s)	528
BsaHI	GRCGYC	1 cut(s)	384
BsaI	GGTCTC	1 cut(s)	801
BsaJI	CCNNGG	3 cut(s)	46, 1317, 1537
BsaXI	ACNNNNNCTCC	4 cut(s)	77, 107, 1767, 1797
Bsc4I	CCNNNNNNNGG	2 cut(s)	348, 629
Bse1I	ACTGG	3 cut(s)	264, 365, 586
Bse21I	CCTNAGG	1 cut(s)	1504
Bse3DI	GCAATG	3 cut(s)	658, 1165, 1825
Bse8I	GATNNNNATC	1 cut(s)	528
BseBI	CCWGG	2 cut(s)	429, 690
BseDI	CCNNGG	3 cut(s)	46, 1317, 1537
BseGI	GGATG	6 cut(s)	157, 577, 994, 1170, 1726, 1864
BseJI	GATNNNNATC	1 cut(s)	528
BseLI	CCNNNNNNNGG	2 cut(s)	348, 629
BseMI	GCAATG	3 cut(s)	658, 1165, 1825
BseMII	CTCAG	8 cut(s)	92, 103, 190, 521, 873, 1329, 1443, 1518
BseNI	ACTGG	3 cut(s)	264, 365, 586
BseRI	GAGGAG	1 cut(s)	85
BseSI	GKGCMC	1 cut(s)	881
BseXI	GCAGC	9 cut(s)	193, 560, 998, 1057, 1189, 1217, 1393, 1585, 1921
BsgI	GTGCAG	4 cut(s)	341, 1272, 1887, 1920
Bsh1236I	CGCG	2 cut(s)	58, 218
BshFI	GGCC	2 cut(s)	135, 424
BshNI	GGYRCC	1 cut(s)	60
BsiHKAI	GWGCWC	1 cut(s)	1587
BsiSI	CCGG	4 cut(s)	46, 136, 154, 342
BslFI	GGGAC	1 cut(s)	1522
BslI	CCNNNNNNNGG	2 cut(s)	348, 629
BsmAI	GTCTC	3 cut(s)	801, 878, 1727
BsmFI	GGGAC	1 cut(s)	1522
BsmI	GAATGC	3 cut(s)	307, 1380, 1745
BsnI	GGCC	2 cut(s)	135, 424
Bso31I	GGTCTC	1 cut(s)	801
Bsp1286I	GDGCHC	2 cut(s)	881, 1587
Bsp143I	GATC	8 cut(s)	273, 289, 400, 925, 1216, 1861, 1882, 1948
BspACI	CCGC	7 cut(s)	58, 198, 216, 998, 1101, 1795, 1886
BspANI	GGCC	2 cut(s)	135, 424
BspCNI	CTCAG	8 cut(s)	93, 102, 189, 520, 874, 1330, 1444, 1517
BspFNI	CGCG	2 cut(s)	58, 218
BspLI	GGNNCC	2 cut(s)	62, 1099
BspMAI	CTGCAG	2 cut(s)	1047, 1207
BspMI	ACCTGC	2 cut(s)	645, 1207
BspPI	GGATC	3 cut(s)	408, 1224, 1877
BspT107I	GGYRCC	1 cut(s)	60
BspTNI	GGTCTC	1 cut(s)	801
BsrDI	GCAATG	3 cut(s)	658, 1165, 1825
BsrI	ACTGG	3 cut(s)	264, 365, 586
BssECI	CCNNGG	3 cut(s)	46, 1317, 1537
BssMI	GATC	8 cut(s)	273, 289, 400, 925, 1216, 1861, 1882, 1948
BssNI	GRCGYC	1 cut(s)	384
BssT1I	CCWWGG	2 cut(s)	1317, 1537
Bst2UI	CCWGG	2 cut(s)	429, 690
Bst6I	CTCTTC	4 cut(s)	30, 342, 601, 1337
BstACI	GRCGYC	1 cut(s)	384
BstAPI	GCANNNNNTGC	1 cut(s)	1874
BstC8I	GCNNGC	1 cut(s)	1997
BstEII	GGTNACC	1 cut(s)	1635
BstF5I	GGATG	6 cut(s)	157, 577, 994, 1170, 1726, 1864
BstFNI	CGCG	2 cut(s)	58, 218
BstHHI	GCGC	3 cut(s)	220, 1187, 1804
BstKTI	GATC	8 cut(s)	276, 292, 403, 928, 1219, 1864, 1885, 1951
BstMAI	GTCTC	3 cut(s)	801, 878, 1727
BstMBI	GATC	8 cut(s)	273, 289, 400, 925, 1216, 1861, 1882, 1948
BstMWI	GCNNNNNNNGC	5 cut(s)	206, 215, 548, 1412, 1874
BstNI	CCWGG	2 cut(s)	429, 690
BstNSI	RCATGY	1 cut(s)	1678
BstPI	GGTNACC	1 cut(s)	1635
BstSCI	CCNGG	4 cut(s)	45, 340, 427, 688
BstSFI	CTRYAG	5 cut(s)	843, 1043, 1203, 1417, 1647
BstSLI	GKGCMC	1 cut(s)	881
BstUI	CGCG	2 cut(s)	58, 218
BstV1I	GCAGC	9 cut(s)	193, 560, 998, 1057, 1189, 1217, 1393, 1585, 1921
BstX2I	RGATCY	1 cut(s)	273
BstXI	CCANNNNNNTGG	1 cut(s)	1757
BstYI	RGATCY	1 cut(s)	273
Bsu36I	CCTNAGG	1 cut(s)	1504
BsuRI	GGCC	2 cut(s)	135, 424
BtsCI	GGATG	6 cut(s)	157, 577, 994, 1170, 1726, 1864
BtsI	GCAGTG	2 cut(s)	162, 397
BtsIMutI	CAGTG	6 cut(s)	37, 162, 358, 397, 1434, 1623
BveI	ACCTGC	2 cut(s)	645, 1207
Cac8I	GCNNGC	1 cut(s)	1997
CaiI	CAGNNNCTG	3 cut(s)	860, 1472, 1655
CfoI	GCGC	3 cut(s)	220, 1187, 1804
Cfr13I	GGNCC	1 cut(s)	1314
CsiI	ACCWGGT	1 cut(s)	427
Csp6I	GTAC	6 cut(s)	333, 598, 1137, 1576, 1672, 1769
CspCI	CAANNNNNGTGG	2 cut(s)	1897, 1932
CviAII	CATG	5 cut(s)	929, 1140, 1579, 1675, 1931
CviQI	GTAC	6 cut(s)	333, 598, 1137, 1576, 1672, 1769
DpnI	GATC	8 cut(s)	275, 291, 402, 927, 1218, 1863, 1884, 1950
DpnII	GATC	8 cut(s)	273, 289, 400, 925, 1216, 1861, 1882, 1948
DraI	TTTAAA	1 cut(s)	1851
EaeI	YGGCCR	1 cut(s)	133
Eam1104I	CTCTTC	4 cut(s)	30, 342, 601, 1337
EarI	CTCTTC	4 cut(s)	30, 342, 601, 1337
EciI	GGCGGA	1 cut(s)	987
Ecl136II	GAGCTC	1 cut(s)	1585
Eco130I	CCWWGG	2 cut(s)	1317, 1537
Eco24I	GRGCYC	1 cut(s)	1587
Eco31I	GGTCTC	1 cut(s)	801
Eco32I	GATATC	1 cut(s)	1978
Eco47I	GGWCC	1 cut(s)	1314
Eco53kI	GAGCTC	1 cut(s)	1585
Eco57I	CTGAAG	5 cut(s)	1245, 1431, 1494, 1686, 1748
Eco81I	CCTNAGG	1 cut(s)	1504
Eco91I	GGTNACC	1 cut(s)	1635
EcoICRI	GAGCTC	1 cut(s)	1585
EcoO109I	RGGNCCY	1 cut(s)	1314
EcoO65I	GGTNACC	1 cut(s)	1635
EcoRI	GAATTC	1 cut(s)	850
EcoRII	CCWGG	2 cut(s)	427, 688
EcoRV	GATATC	1 cut(s)	1978
EcoT14I	CCWWGG	2 cut(s)	1317, 1537
EcoT38I	GRGCYC	1 cut(s)	1587
ErhI	CCWWGG	2 cut(s)	1317, 1537
FaeI	CATG	5 cut(s)	932, 1143, 1582, 1678, 1934
FalI	AAGNNNNNCTT	3 cut(s)	609, 641, 2050
FaqI	GGGAC	1 cut(s)	1522
FatI	CATG	5 cut(s)	928, 1139, 1578, 1674, 1930
FbaI	TGATCA	2 cut(s)	925, 1948
FblI	GTMKAC	1 cut(s)	5
FokI	GGATG	6 cut(s)	144, 584, 981, 1157, 1733, 1871
FriOI	GRGCYC	1 cut(s)	1587
FspBI	CTAG	3 cut(s)	792, 800, 1616
FspI	TGCGCA	1 cut(s)	1803
GlaI	GCGC	3 cut(s)	219, 1186, 1803
GsuI	CTGGAG	1 cut(s)	1687
HaeIII	GGCC	2 cut(s)	135, 424
HapII	CCGG	4 cut(s)	46, 136, 154, 342
HhaI	GCGC	3 cut(s)	220, 1187, 1804
Hin1I	GRCGYC	1 cut(s)	384
Hin1II	CATG	5 cut(s)	932, 1143, 1582, 1678, 1934
Hin6I	GCGC	3 cut(s)	218, 1185, 1802
HinP1I	GCGC	3 cut(s)	218, 1185, 1802
HincII	GTYRAC	1 cut(s)	6
HindII	GTYRAC	1 cut(s)	6
HinfI	GANTC	9 cut(s)	316, 352, 731, 767, 895, 1345, 1553, 1652, 2038
HpaII	CCGG	4 cut(s)	46, 136, 154, 342
HphI	GGTGA	5 cut(s)	22, 1188, 1333, 1653, 1784
Hpy166II	GTNNAC	2 cut(s)	6, 1083
Hpy188I	TCNGA	8 cut(s)	114, 179, 309, 1225, 1339, 1430, 1468, 1698
Hpy8I	GTNNAC	2 cut(s)	6, 1083
Hpy99I	CGWCG	2 cut(s)	10, 389
HpyAV	CCTTC	7 cut(s)	797, 1088, 1220, 1661, 1723, 1735, 1966
HpyCH4IV	ACGT	2 cut(s)	384, 1069
HpyF10VI	GCNNNNNNNGC	5 cut(s)	206, 215, 548, 1412, 1874
HpySE526I	ACGT	2 cut(s)	384, 1069
Hsp92I	GRCGYC	1 cut(s)	384
Hsp92II	CATG	5 cut(s)	932, 1143, 1582, 1678, 1934
HspAI	GCGC	3 cut(s)	218, 1185, 1802
Ksp22I	TGATCA	2 cut(s)	925, 1948
Kzo9I	GATC	8 cut(s)	273, 289, 400, 925, 1216, 1861, 1882, 1948
LmnI	GCTCC	6 cut(s)	98, 138, 662, 692, 1058, 1582
Lsp1109I	GCAGC	9 cut(s)	193, 560, 998, 1057, 1189, 1217, 1393, 1585, 1921
LweI	GCATC	3 cut(s)	166, 491, 952
MabI	ACCWGGT	1 cut(s)	427
MaeI	CTAG	3 cut(s)	792, 800, 1616
MaeII	ACGT	2 cut(s)	384, 1069
MaeIII	GTNAC	3 cut(s)	856, 1635, 1659
MalI	GATC	8 cut(s)	275, 291, 402, 927, 1218, 1863, 1884, 1950
MboI	GATC	8 cut(s)	273, 289, 400, 925, 1216, 1861, 1882, 1948
MfeI	CAATTG	1 cut(s)	543
MflI	RGATCY	1 cut(s)	273
MhlI	GDGCHC	2 cut(s)	881, 1587
MlyI	GAGTC	1 cut(s)	1354
MmeI	TCCRAC	3 cut(s)	472, 640, 735
MroXI	GAANNNNTTC	3 cut(s)	237, 1380, 1745
MseI	TTAA	5 cut(s)	444, 1545, 1850, 2006, 2063
MslI	CAYNNNNRTG	1 cut(s)	1797
MspA1I	CMGCKG	1 cut(s)	1797
MspI	CCGG	4 cut(s)	46, 136, 154, 342
MspR9I	CCNGG	4 cut(s)	47, 342, 429, 690
MunI	CAATTG	1 cut(s)	543
Mva1269I	GAATGC	3 cut(s)	307, 1380, 1745
MvaI	CCWGG	2 cut(s)	429, 690
MvnI	CGCG	2 cut(s)	58, 218
MwoI	GCNNNNNNNGC	5 cut(s)	206, 215, 548, 1412, 1874
NciI	CCSGG	2 cut(s)	47, 342
NdeII	GATC	8 cut(s)	273, 289, 400, 925, 1216, 1861, 1882, 1948
NlaIII	CATG	5 cut(s)	932, 1143, 1582, 1678, 1934
NlaIV	GGNNCC	2 cut(s)	62, 1099
NmuCI	GTSAC	1 cut(s)	1659
NsbI	TGCGCA	1 cut(s)	1803
NspI	RCATGY	1 cut(s)	1678
OliI	CACNNNNGTG	1 cut(s)	1797
PaqCI	CACCTGC	1 cut(s)	1207
PctI	GAATGC	3 cut(s)	307, 1380, 1745
PdmI	GAANNNNTTC	3 cut(s)	237, 1380, 1745
PfeI	GAWTC	8 cut(s)	316, 352, 731, 767, 895, 1553, 1652, 2038
PfoI	TCCNGGA	1 cut(s)	340
PleI	GAGTC	1 cut(s)	1353
PpsI	GAGTC	1 cut(s)	1353
PpuMI	RGGWCCY	1 cut(s)	1314
Psp124BI	GAGCTC	1 cut(s)	1587
Psp5II	RGGWCCY	1 cut(s)	1314
Psp6I	CCWGG	2 cut(s)	427, 688
PspEI	GGTNACC	1 cut(s)	1635
PspGI	CCWGG	2 cut(s)	427, 688
PspN4I	GGNNCC	2 cut(s)	62, 1099
PspPI	GGNCC	1 cut(s)	1314
PspPPI	RGGWCCY	1 cut(s)	1314
PstI	CTGCAG	2 cut(s)	1047, 1207
PstNI	CAGNNNCTG	3 cut(s)	860, 1472, 1655
PsuI	RGATCY	1 cut(s)	273
RsaI	GTAC	6 cut(s)	334, 599, 1138, 1577, 1673, 1770
RsaNI	GTAC	6 cut(s)	333, 598, 1137, 1576, 1672, 1769
RseI	CAYNNNNRTG	1 cut(s)	1797
SacI	GAGCTC	1 cut(s)	1587
SalI	GTCGAC	1 cut(s)	4
SaqAI	TTAA	5 cut(s)	444, 1545, 1850, 2006, 2063
Sau3AI	GATC	8 cut(s)	273, 289, 400, 925, 1216, 1861, 1882, 1948
Sau96I	GGNCC	1 cut(s)	1314
ScaI	AGTACT	1 cut(s)	1770
SchI	GAGTC	1 cut(s)	1354
ScrFI	CCNGG	4 cut(s)	47, 342, 429, 690
SduI	GDGCHC	2 cut(s)	881, 1587
SexAI	ACCWGGT	1 cut(s)	427
SfaNI	GCATC	3 cut(s)	166, 491, 952
SfcI	CTRYAG	5 cut(s)	843, 1043, 1203, 1417, 1647
SinI	GGWCC	1 cut(s)	1314
SmiMI	CAYNNNNRTG	1 cut(s)	1797
SmlI	CTYRAG	1 cut(s)	245
SmoI	CTYRAG	1 cut(s)	245
SsiI	CCGC	7 cut(s)	58, 198, 216, 998, 1101, 1795, 1886
SspI	AATATT	1 cut(s)	1530
SspMI	CTAG	3 cut(s)	792, 800, 1616
SstI	GAGCTC	1 cut(s)	1587
StyD4I	CCNGG	4 cut(s)	45, 340, 427, 688
StyI	CCWWGG	2 cut(s)	1317, 1537
TaiI	ACGT	2 cut(s)	387, 1072
TaqI	TCGA	4 cut(s)	5, 1039, 1239, 1348
TatI	WGTACW	4 cut(s)	332, 597, 1136, 1768
TauI	GCSGC	1 cut(s)	61
TfiI	GAWTC	8 cut(s)	316, 352, 731, 767, 895, 1553, 1652, 2038
Tru1I	TTAA	5 cut(s)	444, 1545, 1850, 2006, 2063
Tru9I	TTAA	5 cut(s)	444, 1545, 1850, 2006, 2063
TscAI	CASTG	6 cut(s)	44, 169, 365, 397, 1441, 1630
TseFI	GTSAC	1 cut(s)	1659
TseI	GCWGC	9 cut(s)	206, 548, 986, 1045, 1202, 1205, 1406, 1598, 1934
Tsp45I	GTSAC	1 cut(s)	1659
TspDTI	ATGAA	9 cut(s)	429, 518, 588, 929, 1107, 1281, 1320, 1737, 1919
TspGWI	ACGGA	1 cut(s)	929
TspRI	CASTG	6 cut(s)	44, 169, 365, 397, 1441, 1630
VpaK11BI	GGWCC	1 cut(s)	1314
XapI	RAATTY	3 cut(s)	556, 850, 978
XbaI	TCTAGA	1 cut(s)	799
XceI	RCATGY	1 cut(s)	1678
XmiI	GTMKAC	1 cut(s)	5
XmnI	GAANNNNTTC	3 cut(s)	237, 1380, 1745
XspI	CTAG	3 cut(s)	792, 800, 1616
ZraI	GACGTC	1 cut(s)	385
ZrmI	AGTACT	1 cut(s)	1770

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.