Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb4

Orthogroup ID

OG0000937

Physical Location & Seq

Genomic Coordinates Reverse (-)

30808068 .. 30815534

Gene Structure 7467 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_4g31910.t2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2685 bp

ATGTCGACGGTGAGCATTGAGCCTCTTCTCCACCACTGCCGCCGTGGAAACTCCCGCCACCGGTCAGCTTCATCCTCGAAGCTGATCAAGCTCAGCTACTTGAGCGCGTTTCCGAAAAAGGTGGAGGAGCTTCGTGGCCGGAGCTTCTGCAACTTCCGGCGTCCGACTCCTCTCACTCTCAGAGCTTCCTCTGCTGACGCCGCCGTAGCTGCGACGTTTGAGTCCACAAAACCTTTCTTCAAGCAGACATTTCCTCTGGAGAGAACTGAGCTGGTGGAGGGAAAGATCTATGTGAGGTTGGATCACGGGAAGAATGATCGGAATTGGACGCTTACAGTTGGTTGCACTCTTCCCGGGAAGTGGGTTCTTCACTGGGGAGTTTCACATGTTGATGATGACGTCGTCAGTGAATGGGAACAACCTCCTGAGGAGATGAGGCCACCTGGTTCGATTCCAATCAAGGACTATGCAATAGACACACCCTTGACGAAATTATCATCAGCTGTTGGAGGTGATAATTCTCAGGAAGTGAAGATTGATTTTAATCTCGATGGTGCAATTGCAGCCATAAATTTCATTCTAAAGGATGAAGAAACTGGTGCTTGTTATCAGCACAGAGGAAGAGATTTCAAAGTGCCTCTTGTGGGCTACCTGCAAGAAGAAAAAGGCAATGTTGTTGGAGCAAAAAAGGGATTGGGGATGTTGCCAGAAATATCAAACTCCCAAGAAAAAGATAGCGGTGGTGAATCCAGAGGAACTAAAGAGCAAACTAGGTCTCTAGAAGGCTTCTATGAAGAACTCCCTATCGCAAAAGAAATTGCTGTAGTGAACTCAGTAACTGTTTCTGTTAGGAAGTGCCCTGAGACAGCAAAGAATCTTCTATATTTGGAAACGGATTTACTTAATCATGTTGTTGTTCATTGGGGAGTTTGCAAAGATGATTCTAAAAGATGGGAAGTTCCAGCTGCCCCACATCCACCAGAAACAGTAGTATTCAAGGATAAGGCTCTGCGAACTCGGTTGCAGCAAAAAGAGGGAGGAAATGGATGTTGGGGACTGTTTACTTTAGAAGAAGGACCCGCAGGATTTCTTTTTGTTTTCAAGCTAAATGAAAGCACATGGTTGAAATGTAAGGGCAATGACTTCTACATCCCCCTATCAAGTGCAAATAAGTTACCTGCTGTAGCGAAAGATGATCACTCTGAAGGTGATAAGGTAGATGAGAGAAGTGAAGAAGAAATAGAAGAAAGTTCTTTTACTGAATTTACCAATGGAATAATAAATGAAATAAGGACCCTGGTGAGTGGCATTTCCTCTGAGAAGAGCCGAAAGACGACATCCAAAGAAGCACAAGAAAGCATTCTCCAAGAAATAGAAAAATTGGCTGCGGAAGCCTACAGTATCTTCAGAAGCAATGTTCCCACGTTTACTGAGGAAACCACTTTAGAATCTGAAGAATTGACACCCTCTGTCAAAATATCCTCAGGGACTGGCACCGGTTTTGAAGTACTGTGCCAAGGTTTTAATTGGGAATCTCATAAATCTGGAAGATGGTACATGGAGCTCAAAAGTAAAGCTGCTGAATTGTCATCACTAGGTTTCACTGTGATTTGGTTACCACCTCCTACAGATTCTGTGTCGCCTGAAGGGTACATGCCAACAGATTTGTATAATCTGAACTCCAGATATGGCACTATGGATGAACTGAAGGAGACTGTGAGGGAATTCCATAAAGTTGGTATCAAAGTACTTGGAGATGCTGTTCTAAATCACCGCTGTGCACAGTATCAGAATAAAAATGGTGTATGGAATATATTTGGTGGTCGTTTAAATTGGGATGATCGTGCAGTTGTTGCAGATGATCCACATTTTCAGGGTAGGGGCAACAAAAGTAGTGGAGATAGTTTTCATGCTGCTCCAAACATTGATCATTCGCAAGATTTTGTGAGGAAGGATATCAAAGAATGGTTATGCTGGCTAAGGCATGAAATTGGGTATGATGGATGGAGGCTAGATTTTGTTAGAGGATTTTGGGGTGGTTATGTTAAGGACTACATGGATGCTAGTGAACCCTACTTTGCTGTGGGTGAGTATTGGGATTCCCTAAGTTACACATATGGTGAGATGGATCACAATCAAGATGCACATAGGCAGAGAATTATTGATTGGATTAATGCTACTAGTGGAGCTGCTGGTGCTTTTGATGTAACAACAAAAGGGATCCTTCATGCTGCTCTGGAAAGATGCGAGTATTGGCGATTATCGGATCAGAAGGGAAAACCTCCTGGGGTTGTTGGATGGTGGCCATCTCGTGCTGTTACCTTCATAGAGAATCACGATACTGGTTCTACTCAGGGTCACTGGAGGTTTCCACGTGACAAGGAAATTCAAGGATACGCTTACACTCTGACTCATCCAGGAACACCAGCAGTCTTCTATGACCACATCTTCTCTCACTACCGATCGGAAATTGCAGGTCTAATCTCTCTAAGAAACCGGAACAAAATCAACTGTCGCAGCATTGTTAAAATTACCAAGGCCGAAAGAGATGTGTATGCGGCTATCATTGATAAAAAAGTTGCAATGAAGATCGGACCAGGTCACTATGAACCCCCAAACGGGGATCAAAAATGGTCCAAAAGCCTCGAAGGAAGAGACTACAAGGTCTGGGAATTAGCAGCATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000272 GO:0003674 GO:0003824 GO:0004553 GO:0004556 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0005976 GO:0005982 GO:0005983 GO:0005984 GO:0005985 GO:0005987 GO:0006073 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009251 GO:0009311 GO:0009313 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009987 GO:0016052 GO:0016160 GO:0016787 GO:0016798 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044042 GO:0044237 GO:0044238 GO:0044247 GO:0044248 GO:0044260 GO:0044262 GO:0044264 GO:0044275 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046352 GO:0071704 GO:1901575

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Alpha-amyl_C2 Alpha-amylase

Protein Analysis

895

Amino Acids

100.78

Weight (kDa)

6.03

Isoelectric Point (pI)

44.95

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ig_N_CWD1	PF23166	91 - 214	2.7e-26	Alpha-glucan water dikinase 1 Ig-like N-terminal domain
Ig_N_CWD1	PF23166	282 - 399	1.3e-33	Alpha-glucan water dikinase 1 Ig-like N-terminal domain
Alpha-amylase	PF00128	542 - 609	4.5e-13	Alpha amylase, catalytic domain
Alpha-amyl_C2	PF07821	846 - 904	2.4e-20	Alpha-amylase C-terminal beta-sheet domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0000937)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G69830
fragaria_vesca	FvH4_4g31910 FvH4_4g31910
malus_domestica	MD13G1078600.v1.1 MD16G1078200.v1.1
prunus_persica	Prupe.1G268300_v2.0.a1 Prupe.1G268300_v2.0.a1
pyrus_communis	pycom13g06820 pycom16g06770
rosa_chinensis	RchiOBHm_Chr1g0315221 RchiOBHm_Chr1g0339091 RchiOBHm_Chr4g0440561 RchiOBHm_Chr5g0021421 RchiOBHm_Chr6g0272311
rosa_laevigata	RLG00000006168 RLG00000015039 RLG00000019642 RLG00000030692
rosa_multiflora	Rmu_co8071990.1_g000001 Rmu_sc0000829.1_g000011 Rmu_sc0002387.1_g000011 Rmu_sc0011312.1_g000002
rosa_roxburghii	Rroxscaffold_4G00298760 Rroxscaffold_5G00381380 Rroxscaffold_6G00426500 Rroxscaffold_7G00169140 Rroxscaffold_7G00214180
rosa_rugosa	Rorug01G0004200 Rorug04G0326700 Rorug05G0356300 Rorug05G0477400 Rorug05G0527600 Rorug07G0284000
rosa_samantha	Rh1AG270700 Rh1AG270800 Rh1AG346400 Rh1BG260100 Rh1CG254500 Rh2AG416800 Rh2CG403000 Rh3DG074600 Rh3DG292400 Rh4AG190200 Rh4AG246600 Rh4AG378400 Rh4BG035500 Rh4BG391100 Rh4CG405800 Rh4DG085900 Rh4DG385000 Rh5AG156300 Rh5AG268600 Rh5AG480700 Rh5BG155700 Rh5CG169500 Rh6AG024000 Rh6BG089200 Rh6DG025600 Rh7CG251800
rosa_wichuraiana	Rw1G024120 Rw1G030800 Rw4G032400 Rw4G032500 Rw7G034860

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	220, 2663
AatII	GACGTC	1 cut(s)	402
Acc36I	ACCTGC	3 cut(s)	660, 1186, 2465
AccB1I	GGYRCC	1 cut(s)	1493
AccI	GTMKAC	1 cut(s)	5
AccII	CGCG	1 cut(s)	107
AciI	CCGC	8 cut(s)	40, 55, 201, 738, 1080, 1388, 1774, 2558
AclWI	GGATC	7 cut(s)	309, 1856, 2136, 2215, 2228, 2274, 2631
AcoI	YGGCCR	2 cut(s)	136, 2303
AcsI	RAATTY	4 cut(s)	571, 1262, 1724, 2385
AcuI	CTGAAG	5 cut(s)	1224, 1390, 1473, 1665, 1727
AcvI	CACGTG	1 cut(s)	2375
AcyI	GRCGYC	3 cut(s)	160, 198, 399
AfaI	GTAC	4 cut(s)	1509, 1556, 1652, 1749
AfiI	CCNNNNNNNGG	6 cut(s)	60, 360, 644, 2618, 2619, 2620
AflIII	ACRYGT	1 cut(s)	385
AgeI	ACCGGT	2 cut(s)	60, 1496
AgsI	TTSAA	7 cut(s)	241, 631, 997, 1102, 1126, 1505, 2390
AhlI	ACTAGT	1 cut(s)	2180
AjnI	CCWGG	5 cut(s)	442, 1296, 2284, 2416, 2596
AjuI	GAANNNNNNNTTGG	4 cut(s)	1033, 1065, 2149, 2181
AleI	CACNNNNGTG	1 cut(s)	1776
AloI	GAACNNNNNNTCC	2 cut(s)	1746, 1778
Alw21I	GWGCWC	2 cut(s)	1566, 1783
Alw26I	GTCTC	4 cut(s)	780, 857, 1706, 2649
Alw44I	GTGCAC	1 cut(s)	1779
AlwI	GGATC	7 cut(s)	309, 1856, 2136, 2215, 2228, 2274, 2631
AlwNI	CAGNNNCTG	3 cut(s)	839, 1490, 1634
Ama87I	CYCGRG	1 cut(s)	353
AoxI	GGCC	4 cut(s)	136, 437, 2303, 2538
ApaLI	GTGCAC	1 cut(s)	1779
ApoI	RAATTY	4 cut(s)	571, 1262, 1724, 2385
AseI	ATTAAT	1 cut(s)	2172
AsiGI	ACCGGT	2 cut(s)	60, 1496
Asp700I	GAANNNNTTC	1 cut(s)	1359
AspLEI	GCGC	1 cut(s)	107
AspS9I	GGNCC	4 cut(s)	1076, 1293, 2594, 2634
AsuC2I	CCSGG	2 cut(s)	354, 355
AsuHPI	GGTGA	8 cut(s)	22, 524, 755, 1220, 1312, 1763, 2099, 2132
AvaI	CYCGRG	1 cut(s)	353
AvaII	GGWCC	4 cut(s)	1076, 1293, 2594, 2634
AxyI	CCTNAGG	2 cut(s)	426, 1483
BaeGI	GKGCMC	2 cut(s)	860, 1783
BalI	TGGCCA	1 cut(s)	2305
BamHI	GGATCC	1 cut(s)	2220
BanI	GGYRCC	1 cut(s)	1493
BanII	GRGCYC	1 cut(s)	1566
BauI	CACGAG	1 cut(s)	2310
BbrPI	CACGTG	1 cut(s)	2375
BbsI	GAAGAC	1 cut(s)	2425
Bbv12I	GWGCWC	2 cut(s)	1566, 1783
BccI	CCATC	8 cut(s)	545, 945, 1545, 1994, 1998, 2119, 2292, 2314
BceAI	ACGGC	2 cut(s)	27, 188
BciT130I	CCWGG	5 cut(s)	444, 1298, 2286, 2418, 2598
BciVI	GTATCC	1 cut(s)	2387
BclI	TGATCA	3 cut(s)	84, 1195, 1927
BcnI	CCSGG	2 cut(s)	354, 355
BcoDI	GTCTC	4 cut(s)	780, 857, 1706, 2649
BcuI	ACTAGT	1 cut(s)	2180
BfaI	CTAG	6 cut(s)	771, 779, 1595, 2012, 2064, 2181
BfmI	CTRYAG	4 cut(s)	822, 1182, 1396, 1626
BfuAI	ACCTGC	3 cut(s)	660, 1186, 2465
BfuI	GTATCC	1 cut(s)	2387
BglII	AGATCT	1 cut(s)	285
BlpI	GCTNAGC	1 cut(s)	92
BmcAI	AGTACT	2 cut(s)	1509, 1749
Bme1390I	CCNGG	7 cut(s)	354, 355, 444, 1298, 2286, 2418, 2598
Bme18I	GGWCC	4 cut(s)	1076, 1293, 2594, 2634
BmeT110I	CYCGRG	1 cut(s)	353
BmgT120I	GGNCC	4 cut(s)	1076, 1293, 2594, 2634
BmiI	GGNNCC	4 cut(s)	1078, 1295, 1495, 2222
BmrFI	CCNGG	7 cut(s)	354, 355, 444, 1298, 2286, 2418, 2598
BmrI	ACTGGG	1 cut(s)	382
BmsI	GCATC	4 cut(s)	1747, 2050, 2131, 2234
BmuI	ACTGGG	1 cut(s)	382
BpiI	GAAGAC	1 cut(s)	2425
BpmI	CTGGAG	3 cut(s)	278, 1666, 2383
Bpu10I	CCTNAGC	1 cut(s)	1979
Bpu1102I	GCTNAGC	1 cut(s)	92
BpuEI	CTTGAG	1 cut(s)	121
BpuMI	CCSGG	2 cut(s)	354, 355
BsaAI	YACGTR	1 cut(s)	2375
BsaBI	GATNNNNATC	3 cut(s)	455, 543, 2133
BsaHI	GRCGYC	3 cut(s)	160, 198, 399
BsaI	GGTCTC	1 cut(s)	780
BsaJI	CCNNGG	6 cut(s)	43, 353, 1296, 1516, 2285, 2535
BsaWI	WCCGGW	3 cut(s)	60, 1496, 2496
BsaXI	ACNNNNNCTCC	2 cut(s)	1746, 1776
Bsc4I	CCNNNNNNNGG	6 cut(s)	60, 360, 644, 2618, 2619, 2620
Bse118I	RCCGGY	2 cut(s)	60, 1496
Bse1I	ACTGG	5 cut(s)	377, 601, 1495, 2347, 2366
Bse21I	CCTNAGG	2 cut(s)	426, 1483
Bse3DI	GCAATG	4 cut(s)	676, 1144, 1420, 2589
Bse8I	GATNNNNATC	3 cut(s)	455, 543, 2133
BseBI	CCWGG	5 cut(s)	444, 1298, 2286, 2418, 2598
BseDI	CCNNGG	6 cut(s)	43, 353, 1296, 1516, 2285, 2535
BseJI	GATNNNNATC	3 cut(s)	455, 543, 2133
BseLI	CCNNNNNNNGG	6 cut(s)	60, 360, 644, 2618, 2619, 2620
BseMI	GCAATG	4 cut(s)	676, 1144, 1420, 2589
BseNI	ACTGG	5 cut(s)	377, 601, 1495, 2347, 2366
BseRI	GAGGAG	3 cut(s)	140, 159, 443
BseSI	GKGCMC	2 cut(s)	860, 1783
BsgI	GTGCAG	1 cut(s)	1866
Bsh1236I	CGCG	1 cut(s)	107
Bsh1285I	CGRYCG	1 cut(s)	2465
BshFI	GGCC	4 cut(s)	138, 439, 2305, 2540
BshNI	GGYRCC	1 cut(s)	1493
BshTI	ACCGGT	2 cut(s)	60, 1496
BsiEI	CGRYCG	1 cut(s)	2465
BsiHKAI	GWGCWC	2 cut(s)	1566, 1783
BsiHKCI	CYCGRG	1 cut(s)	353
BsiSI	CCGG	6 cut(s)	61, 139, 157, 354, 1497, 2497
BslFI	GGGAC	2 cut(s)	1068, 1501
BslI	CCNNNNNNNGG	6 cut(s)	60, 360, 644, 2618, 2619, 2620
BsmAI	GTCTC	4 cut(s)	780, 857, 1706, 2649
BsmFI	GGGAC	2 cut(s)	1068, 1501
BsmI	GAATGC	1 cut(s)	1359
BsnI	GGCC	4 cut(s)	138, 439, 2305, 2540
Bso31I	GGTCTC	1 cut(s)	780
BsoBI	CYCGRG	1 cut(s)	353
Bsp1286I	GDGCHC	3 cut(s)	860, 1566, 1783
Bsp1720I	GCTNAGC	1 cut(s)	92
BspACI	CCGC	8 cut(s)	40, 55, 201, 738, 1080, 1388, 1774, 2558
BspANI	GGCC	4 cut(s)	138, 439, 2305, 2540
BspFNI	CGCG	1 cut(s)	107
BspLI	GGNNCC	4 cut(s)	1078, 1295, 1495, 2222
BspMI	ACCTGC	3 cut(s)	660, 1186, 2465
BspPI	GGATC	7 cut(s)	309, 1856, 2136, 2215, 2228, 2274, 2631
BspQI	GCTCTTC	1 cut(s)	1316
BspT107I	GGYRCC	1 cut(s)	1493
BspTNI	GGTCTC	1 cut(s)	780
BsrDI	GCAATG	4 cut(s)	676, 1144, 1420, 2589
BsrFI	RCCGGY	2 cut(s)	60, 1496
BsrI	ACTGG	5 cut(s)	377, 601, 1495, 2347, 2366
BssAI	RCCGGY	2 cut(s)	60, 1496
BssECI	CCNNGG	6 cut(s)	43, 353, 1296, 1516, 2285, 2535
BssNI	GRCGYC	3 cut(s)	160, 198, 399
BssSI	CACGAG	1 cut(s)	2310
BssT1I	CCWWGG	2 cut(s)	1516, 2535
Bst2BI	CACGAG	1 cut(s)	2310
Bst2UI	CCWGG	5 cut(s)	444, 1298, 2286, 2418, 2598
Bst6I	CTCTTC	5 cut(s)	30, 354, 616, 1316, 2647
BstACI	GRCGYC	3 cut(s)	160, 198, 399
BstAPI	GCANNNNNTGC	1 cut(s)	1853
BstBAI	YACGTR	1 cut(s)	2375
BstC8I	GCNNGC	1 cut(s)	1976
BstDSI	CCRYGG	1 cut(s)	43
BstEII	GGTNACC	1 cut(s)	1614
BstFNI	CGCG	1 cut(s)	107
BstHHI	GCGC	1 cut(s)	107
BstMAI	GTCTC	4 cut(s)	780, 857, 1706, 2649
BstMCI	CGRYCG	1 cut(s)	2465
BstNI	CCWGG	5 cut(s)	444, 1298, 2286, 2418, 2598
BstNSI	RCATGY	2 cut(s)	389, 1657
BstPI	GGTNACC	1 cut(s)	1614
BstSCI	CCNGG	7 cut(s)	352, 353, 442, 1296, 2284, 2416, 2596
BstSFI	CTRYAG	4 cut(s)	822, 1182, 1396, 1626
BstSLI	GKGCMC	2 cut(s)	860, 1783
BstUI	CGCG	1 cut(s)	107
BstV2I	GAAGAC	1 cut(s)	2425
BstX2I	RGATCY	2 cut(s)	285, 2220
BstXI	CCANNNNNNTGG	1 cut(s)	1736
BstYI	RGATCY	2 cut(s)	285, 2220
Bsu36I	CCTNAGG	2 cut(s)	426, 1483
BsuI	GTATCC	1 cut(s)	2387
BsuRI	GGCC	4 cut(s)	138, 439, 2305, 2540
BtgI	CCRYGG	1 cut(s)	43
BtsI	GCAGTG	1 cut(s)	34
BtsIMutI	CAGTG	5 cut(s)	34, 370, 412, 1602, 2359
BveI	ACCTGC	3 cut(s)	660, 1186, 2465
Cac8I	GCNNGC	1 cut(s)	1976
CaiI	CAGNNNCTG	3 cut(s)	839, 1490, 1634
CfoI	GCGC	1 cut(s)	107
Cfr10I	RCCGGY	2 cut(s)	60, 1496
Cfr13I	GGNCC	4 cut(s)	1076, 1293, 2594, 2634
Cfr9I	CCCGGG	1 cut(s)	353
CseI	GACGC	3 cut(s)	149, 206, 337
CsiI	ACCWGGT	2 cut(s)	442, 2596
Csp6I	GTAC	4 cut(s)	1508, 1555, 1651, 1748
CspAI	ACCGGT	2 cut(s)	60, 1496
CspCI	CAANNNNNGTGG	2 cut(s)	1876, 1911
CviAII	CATG	9 cut(s)	386, 908, 1119, 1558, 1654, 1910, 1985, 2056, 2228
CviQI	GTAC	4 cut(s)	1508, 1555, 1651, 1748
DraI	TTTAAA	1 cut(s)	1830
DrdI	GACNNNNNNGTC	2 cut(s)	220, 2663
DseDI	GACNNNNNNGTC	2 cut(s)	220, 2663
EaeI	YGGCCR	2 cut(s)	136, 2303
Eam1104I	CTCTTC	5 cut(s)	30, 354, 616, 1316, 2647
EarI	CTCTTC	5 cut(s)	30, 354, 616, 1316, 2647
Ecl136II	GAGCTC	1 cut(s)	1564
Eco130I	CCWWGG	2 cut(s)	1516, 2535
Eco24I	GRGCYC	1 cut(s)	1566
Eco31I	GGTCTC	1 cut(s)	780
Eco32I	GATATC	1 cut(s)	1957
Eco47I	GGWCC	4 cut(s)	1076, 1293, 2594, 2634
Eco53kI	GAGCTC	1 cut(s)	1564
Eco57I	CTGAAG	5 cut(s)	1224, 1390, 1473, 1665, 1727
Eco72I	CACGTG	1 cut(s)	2375
Eco81I	CCTNAGG	2 cut(s)	426, 1483
Eco88I	CYCGRG	1 cut(s)	353
Eco91I	GGTNACC	1 cut(s)	1614
EcoICRI	GAGCTC	1 cut(s)	1564
EcoO109I	RGGNCCY	2 cut(s)	1076, 1293
EcoO65I	GGTNACC	1 cut(s)	1614
EcoRI	GAATTC	1 cut(s)	1724
EcoRII	CCWGG	5 cut(s)	442, 1296, 2284, 2416, 2596
EcoRV	GATATC	1 cut(s)	1957
EcoT14I	CCWWGG	2 cut(s)	1516, 2535
EcoT38I	GRGCYC	1 cut(s)	1566
ErhI	CCWWGG	2 cut(s)	1516, 2535
FaeI	CATG	9 cut(s)	389, 911, 1122, 1561, 1657, 1913, 1988, 2059, 2231
FalI	AAGNNNNNCTT	4 cut(s)	624, 656, 2208, 2240
FaqI	GGGAC	2 cut(s)	1068, 1501
FatI	CATG	9 cut(s)	385, 907, 1118, 1557, 1653, 1909, 1984, 2055, 2227
FauI	CCCGC	2 cut(s)	62, 1087
FauNDI	CATATG	1 cut(s)	2116
FbaI	TGATCA	3 cut(s)	84, 1195, 1927
FblI	GTMKAC	1 cut(s)	5
FriOI	GRGCYC	1 cut(s)	1566
FspBI	CTAG	6 cut(s)	771, 779, 1595, 2012, 2064, 2181
GlaI	GCGC	1 cut(s)	106
GsuI	CTGGAG	3 cut(s)	278, 1666, 2383
HaeIII	GGCC	4 cut(s)	138, 439, 2305, 2540
HapII	CCGG	6 cut(s)	61, 139, 157, 354, 1497, 2497
HgaI	GACGC	3 cut(s)	149, 206, 337
HhaI	GCGC	1 cut(s)	107
Hin1I	GRCGYC	3 cut(s)	160, 198, 399
Hin1II	CATG	9 cut(s)	389, 911, 1122, 1561, 1657, 1913, 1988, 2059, 2231
Hin6I	GCGC	1 cut(s)	105
HinP1I	GCGC	1 cut(s)	105
HincII	GTYRAC	1 cut(s)	6
HindII	GTYRAC	1 cut(s)	6
HpaII	CCGG	6 cut(s)	61, 139, 157, 354, 1497, 2497
HphI	GGTGA	8 cut(s)	22, 524, 755, 1220, 1312, 1763, 2099, 2132
Hpy166II	GTNNAC	7 cut(s)	6, 225, 829, 1062, 1428, 1781, 2069
Hpy8I	GTNNAC	7 cut(s)	6, 225, 829, 1062, 1428, 1781, 2069
Hpy99I	CGWCG	3 cut(s)	10, 217, 404
HpyCH4IV	ACGT	4 cut(s)	215, 399, 1424, 2374
HpySE526I	ACGT	4 cut(s)	215, 399, 1424, 2374
Hsp92I	GRCGYC	3 cut(s)	160, 198, 399
Hsp92II	CATG	9 cut(s)	389, 911, 1122, 1561, 1657, 1913, 1988, 2059, 2231
HspAI	GCGC	1 cut(s)	105
Ksp22I	TGATCA	3 cut(s)	84, 1195, 1927
LguI	GCTCTTC	1 cut(s)	1316
LmnI	GCTCC	6 cut(s)	127, 141, 680, 1561, 1921, 2186
LweI	GCATC	4 cut(s)	1747, 2050, 2131, 2234
MabI	ACCWGGT	2 cut(s)	442, 2596
MaeI	CTAG	6 cut(s)	771, 779, 1595, 2012, 2064, 2181
MaeII	ACGT	4 cut(s)	215, 399, 1424, 2374
MaeIII	GTNAC	9 cut(s)	835, 1173, 1614, 2108, 2206, 2317, 2357, 2375, 2600
MfeI	CAATTG	1 cut(s)	558
MflI	RGATCY	2 cut(s)	285, 2220
MhlI	GDGCHC	3 cut(s)	860, 1566, 1783
MlsI	TGGCCA	1 cut(s)	2305
MluNI	TGGCCA	1 cut(s)	2305
MlyI	GAGTC	3 cut(s)	160, 230, 2404
MmeI	TCCRAC	5 cut(s)	188, 279, 487, 658, 2275
Mox20I	TGGCCA	1 cut(s)	2305
MroXI	GAANNNNTTC	1 cut(s)	1359
MscI	TGGCCA	1 cut(s)	2305
MseI	TTAA	7 cut(s)	543, 903, 1524, 1829, 2046, 2172, 2526
MslI	CAYNNNNRTG	2 cut(s)	390, 1776
Msp20I	TGGCCA	1 cut(s)	2305
MspA1I	CMGCKG	3 cut(s)	503, 965, 1776
MspI	CCGG	6 cut(s)	61, 139, 157, 354, 1497, 2497
MspR9I	CCNGG	7 cut(s)	354, 355, 444, 1298, 2286, 2418, 2598
MunI	CAATTG	1 cut(s)	558
Mva1269I	GAATGC	1 cut(s)	1359
MvaI	CCWGG	5 cut(s)	444, 1298, 2286, 2418, 2598
MvnI	CGCG	1 cut(s)	107
NciI	CCSGG	2 cut(s)	354, 355
NdeI	CATATG	1 cut(s)	2116
NlaIII	CATG	9 cut(s)	389, 911, 1122, 1561, 1657, 1913, 1988, 2059, 2231
NlaIV	GGNNCC	4 cut(s)	1078, 1295, 1495, 2222
NmuCI	GTSAC	3 cut(s)	2357, 2375, 2600
NspI	RCATGY	2 cut(s)	389, 1657
OliI	CACNNNNGTG	1 cut(s)	1776
PciI	ACATGT	1 cut(s)	385
PciSI	GCTCTTC	1 cut(s)	1316
PctI	GAATGC	1 cut(s)	1359
PdmI	GAANNNNTTC	1 cut(s)	1359
PfeI	GAWTC	9 cut(s)	451, 746, 874, 941, 1448, 1532, 1631, 2099, 2332
PflFI	GACNNNGTC	2 cut(s)	401, 2598
PfoI	TCCNGGA	1 cut(s)	2416
PinAI	ACCGGT	2 cut(s)	60, 1496
Ple19I	CGATCG	1 cut(s)	2465
PleI	GAGTC	3 cut(s)	160, 229, 2404
PmaCI	CACGTG	1 cut(s)	2375
PmlI	CACGTG	1 cut(s)	2375
PpsI	GAGTC	3 cut(s)	160, 229, 2404
Ppu21I	YACGTR	1 cut(s)	2375
PpuMI	RGGWCCY	2 cut(s)	1076, 1293
PscI	ACATGT	1 cut(s)	385
PshBI	ATTAAT	1 cut(s)	2172
Psp124BI	GAGCTC	1 cut(s)	1566
Psp5II	RGGWCCY	2 cut(s)	1076, 1293
Psp6I	CCWGG	5 cut(s)	442, 1296, 2284, 2416, 2596
PspCI	CACGTG	1 cut(s)	2375
PspEI	GGTNACC	1 cut(s)	1614
PspGI	CCWGG	5 cut(s)	442, 1296, 2284, 2416, 2596
PspN4I	GGNNCC	4 cut(s)	1078, 1295, 1495, 2222
PspPI	GGNCC	4 cut(s)	1076, 1293, 2594, 2634
PspPPI	RGGWCCY	2 cut(s)	1076, 1293
PstNI	CAGNNNCTG	3 cut(s)	839, 1490, 1634
PsuI	RGATCY	2 cut(s)	285, 2220
PsyI	GACNNNGTC	2 cut(s)	401, 2598
PvuI	CGATCG	1 cut(s)	2465
PvuII	CAGCTG	2 cut(s)	503, 965
RsaI	GTAC	4 cut(s)	1509, 1556, 1652, 1749
RsaNI	GTAC	4 cut(s)	1508, 1555, 1651, 1748
RseI	CAYNNNNRTG	2 cut(s)	390, 1776
SacI	GAGCTC	1 cut(s)	1566
SalI	GTCGAC	1 cut(s)	4
SapI	GCTCTTC	1 cut(s)	1316
SaqAI	TTAA	7 cut(s)	543, 903, 1524, 1829, 2046, 2172, 2526
Sau96I	GGNCC	4 cut(s)	1076, 1293, 2594, 2634
ScaI	AGTACT	2 cut(s)	1509, 1749
SchI	GAGTC	3 cut(s)	160, 230, 2404
ScrFI	CCNGG	7 cut(s)	354, 355, 444, 1298, 2286, 2418, 2598
SduI	GDGCHC	3 cut(s)	860, 1566, 1783
SexAI	ACCWGGT	2 cut(s)	442, 2596
SfaNI	GCATC	4 cut(s)	1747, 2050, 2131, 2234
SfcI	CTRYAG	4 cut(s)	822, 1182, 1396, 1626
SinI	GGWCC	4 cut(s)	1076, 1293, 2594, 2634
SmaI	CCCGGG	1 cut(s)	355
SmiMI	CAYNNNNRTG	2 cut(s)	390, 1776
SmlI	CTYRAG	1 cut(s)	100
SmoI	CTYRAG	1 cut(s)	100
SpeI	ACTAGT	1 cut(s)	2180
SsiI	CCGC	8 cut(s)	40, 55, 201, 738, 1080, 1388, 1774, 2558
SspMI	CTAG	6 cut(s)	771, 779, 1595, 2012, 2064, 2181
SstI	GAGCTC	1 cut(s)	1566
StyD4I	CCNGG	7 cut(s)	352, 353, 442, 1296, 2284, 2416, 2596
StyI	CCWWGG	2 cut(s)	1516, 2535
TaiI	ACGT	4 cut(s)	218, 402, 1427, 2377
TaqI	TCGA	5 cut(s)	5, 77, 449, 549, 2646
TatI	WGTACW	2 cut(s)	1507, 1747
TauI	GCSGC	3 cut(s)	42, 203, 2561
TfiI	GAWTC	9 cut(s)	451, 746, 874, 941, 1448, 1532, 1631, 2099, 2332
Tru1I	TTAA	7 cut(s)	543, 903, 1524, 1829, 2046, 2172, 2526
Tru9I	TTAA	7 cut(s)	543, 903, 1524, 1829, 2046, 2172, 2526
TscAI	CASTG	5 cut(s)	41, 377, 412, 1609, 2366
TseFI	GTSAC	3 cut(s)	2357, 2375, 2600
Tsp45I	GTSAC	3 cut(s)	2357, 2375, 2600
TspGWI	ACGGA	1 cut(s)	908
TspMI	CCCGGG	1 cut(s)	353
TspRI	CASTG	5 cut(s)	41, 377, 412, 1609, 2366
Tth111I	GACNNNGTC	2 cut(s)	401, 2598
VneI	GTGCAC	1 cut(s)	1779
VpaK11BI	GGWCC	4 cut(s)	1076, 1293, 2594, 2634
VspI	ATTAAT	1 cut(s)	2172
XapI	RAATTY	4 cut(s)	571, 1262, 1724, 2385
XbaI	TCTAGA	1 cut(s)	778
XceI	RCATGY	2 cut(s)	389, 1657
XcmI	CCANNNNNNNNNTGG	1 cut(s)	41
XmaI	CCCGGG	1 cut(s)	353
XmiI	GTMKAC	1 cut(s)	5
XmnI	GAANNNNTTC	1 cut(s)	1359
XspI	CTAG	6 cut(s)	771, 779, 1595, 2012, 2064, 2181
ZraI	GACGTC	1 cut(s)	400
ZrmI	AGTACT	2 cut(s)	1509, 1749

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.