Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr16

Orthogroup ID

OG0000937

Physical Location & Seq

Genomic Coordinates Reverse (-)

4378574 .. 4383981

Gene Structure 5408 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom16g06770.6

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2244 bp

ATGTCGACGGTTAGGATAGAGCCCCTCCTCCGGGACCTCCACCACTACCGTCGACAAAAACCCAGACACCGCCGTCCGCCGTCGAATCATCCATTAAAGCTCAGCTCTTCTTTCACTGCTTTTCCAAAGAAGCTAGTAGTCTCCAACGGCCGCAGCTTCTGTTACTTCCAGCCTCCCACTCCCAGGAGGGGTCCCACTCTCGGAGTCAGAGCTGCCTCAACCGATACAGCCACTGTCGAGACCTCCGAATCCGCCGATCCCTTCTACAAGAAGACCTTCCCTCTCAAGCGGACGGAAGTGGTGGAGGGAAAGATCGTTGTGAAATTAGATCATGGGAAGAATGAAAAGAAGTGGGTGCTGACTGTGGGTTGTAATCTTCCTGGAAAATGGGTTCTTCACTGGGGAGTTTCTTTTGTGGACGATGTTAGTGGCGAATGGGAACAGCCTCCTAGTGAAATGAGACCAGCTGGTTCAGTTCCCATCAAGGACTATGCAATAGAGACACCCTTGAAGGAATCGTTGTCGTCTGTGGGAGGCGATACATCTTATGAAGTGAAGATTGATGTTAAACCCAACAGTGCAATTGCAGCGATAAATTTTGTTCTCAAGGATGAAGAAACTGGTGCTTGGTATCAGCACAGAAGGAGTGACTTTAGAGTGCCTCTTGTCGCCTACCCACAAGACGATGACAATGTAGTTGGAGAAACAAAGGGCTTCGGCATGTGGCCAGGAGTTTTGGGTAAACTATCCAACGTGTTCGTCAAAGCAGAATCATCAGATTCCAAAGATCAAGACAGCAGCGGCAGTGAATCCAGACACCCTCAACAGAAAACTACGTGTCTAGAAGGATTCTATGAAGAACTGCCAATTGCAAAAGAAATTTCTGTAAACAATTCAGTAACTGTTTCTGCTAGGAAGTGCCCTGAGACAGCTAAGAATCTACTATACTTGGAAACAGATTTACCTGATAATGCTGTTGTTCACTGGGGAGTTTGCAGAGATGATACTAAAAGATGGGAAATTCCAGCTGCCCCGCATCCACCAGAAACAGTAGTATTCAAGGACAAGGCTTTGCGGACTCGATTACAGCAAAAAGAGGGTGGAAATGGATGCTGGGGACTGTTTACTTTGGAAGAAGGACTCGCAGGATTTCTTTTTCTTTTGAAACTAAATGAAAGTACGTGGTTGAGATGTGTGGGAAATGACTTCTACATCCCCCTTTCAAGCTCAAAAAACGCAAATGTTTTACAAGGAGAGATTCAATCTGAAGATGCTCAGGTATCTGACGGAAGTACAGAAGCAGTAGAAGAAAATACTGCATATGCCGATGGATTAATCAATGAAATGAGGAACTTGGTGAGTGACGTTTTCTCTGATAGAAGTCAAAGGACAAGATCCAAAAAAGCACAAGAAGCCATTCTTCAAGAAATAGAAAAATTGGCTGCTGAAGCGTATAGTATCTTCAGAACTACCGTTCCAACTTTACCTGAGGAAACCATTGCAGAAACTGAAGATGTGAAAGTCGCCCCTGCCAAAATATGCTCAGGGACAGGAACAGGTTTCGAAATATTGTGCCAAGGTTTTAACTGGGAATCTAGTAAATCTGGAAGATGGTACATGGAACTCAAAAGTAAAGCTGCAGAGTTATCTTCACTAGGTTTCACTGTGATTTGGTTTCCACCTCCCACAGAGTCTGTTTCACCTCAAGGGTACATGCCCAGGGATTTATATAATCTGAACTCCAGATACGGAAATATGGACGAACTGAAGGAGACCGTGAAGGTATTCCATGACGTCGGGATAAAAGTTCTTGGAGATGCTGTTCTGAATCACCGTTGTGCACATTATCGGAATCAAAATGGTGTTTGGAATATATTTGGTGGTCGTTTAAATTGGGATGATCGTGCAGTTGTTGCAGACGATCCACATTTTCAGGGAAGGGGTAACAAAAGTAGTGGAGATAGTTTTCATGCAGCCCCAAACATTGATCATTCACAAGATTTTGTGAGGAAGGATATCAGAGAATGGTTATGCTGGCTAAGGAACGATATTGGGTACGACGGATGGAGGCTTGATTTTGTTAGAGGATTTTGGGGTGGCTATGTCAGGGACTACATTGATGCTAGTGAGCCCTACTTTGCTGTAGGCGAGTTTTGGGATTCCCTAAGTTATACATACGGGCAAATGGATCGACGATCAAGATGCACACAGGCAGAGAATTGTTGGATCGATCAACGCTACTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000272 GO:0003674 GO:0003824 GO:0004553 GO:0004556 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0005976 GO:0005982 GO:0005983 GO:0005984 GO:0005985 GO:0005987 GO:0006073 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009251 GO:0009311 GO:0009313 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009987 GO:0016052 GO:0016160 GO:0016787 GO:0016798 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044042 GO:0044237 GO:0044238 GO:0044247 GO:0044248 GO:0044260 GO:0044262 GO:0044264 GO:0044275 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046352 GO:0071704 GO:1901575

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Alpha-amyl_C2 Alpha-amylase

Protein Analysis

748

Amino Acids

84.29

Weight (kDa)

5.85

Isoelectric Point (pI)

47.39

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000937)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G69830
fragaria_vesca	FvH4_4g31910 FvH4_4g31910
malus_domestica	MD13G1078600.v1.1 MD16G1078200.v1.1
prunus_persica	Prupe.1G268300_v2.0.a1 Prupe.1G268300_v2.0.a1
pyrus_communis	pycom13g06820 pycom16g06770
rosa_chinensis	RchiOBHm_Chr1g0315221 RchiOBHm_Chr1g0339091 RchiOBHm_Chr4g0440561 RchiOBHm_Chr5g0021421 RchiOBHm_Chr6g0272311
rosa_laevigata	RLG00000006168 RLG00000015039 RLG00000019642 RLG00000030692
rosa_multiflora	Rmu_co8071990.1_g000001 Rmu_sc0000829.1_g000011 Rmu_sc0002387.1_g000011 Rmu_sc0011312.1_g000002
rosa_roxburghii	Rroxscaffold_4G00298760 Rroxscaffold_5G00381380 Rroxscaffold_6G00426500 Rroxscaffold_7G00169140 Rroxscaffold_7G00214180
rosa_rugosa	Rorug01G0004200 Rorug04G0326700 Rorug05G0356300 Rorug05G0477400 Rorug05G0527600 Rorug07G0284000
rosa_samantha	Rh1AG270700 Rh1AG270800 Rh1AG346400 Rh1BG260100 Rh1CG254500 Rh2AG416800 Rh2CG403000 Rh3DG074600 Rh3DG292400 Rh4AG190200 Rh4AG246600 Rh4AG378400 Rh4BG035500 Rh4BG391100 Rh4CG405800 Rh4DG085900 Rh4DG385000 Rh5AG156300 Rh5AG268600 Rh5AG480700 Rh5BG155700 Rh5CG169500 Rh6AG024000 Rh6BG089200 Rh6DG025600 Rh7CG251800
rosa_wichuraiana	Rw1G024120 Rw1G030800 Rw4G032400 Rw4G032500 Rw7G034860

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	72
AatII	GACGTC	1 cut(s)	1797
AccI	GTMKAC	2 cut(s)	5, 52
AciI	CCGC	8 cut(s)	70, 77, 151, 252, 289, 801, 1034, 1075
AclWI	GGATC	5 cut(s)	251, 1389, 1916, 2196, 2234
AcoI	YGGCCR	2 cut(s)	148, 725
AcsI	RAATTY	3 cut(s)	595, 879, 1020
AcuI	CTGAAG	5 cut(s)	1287, 1447, 1467, 1530, 1787
AcyI	GRCGYC	1 cut(s)	1794
AfaI	GTAC	5 cut(s)	1180, 1294, 1616, 1712, 2057
AfiI	CCNNNNNNNGG	5 cut(s)	30, 31, 183, 188, 200
AflIII	ACRYGT	2 cut(s)	753, 836
AgsI	TTSAA	6 cut(s)	511, 1060, 1165, 1224, 1262, 1424
AjnI	CCWGG	4 cut(s)	182, 379, 727, 1718
AleI	CACNNNNGTG	1 cut(s)	1836
AloI	GAACNNNNNNTCC	6 cut(s)	375, 407, 1459, 1491, 1806, 1838
Alw21I	GWGCWC	1 cut(s)	1843
Alw26I	GTCTC	6 cut(s)	145, 233, 454, 494, 920, 1766
Alw44I	GTGCAC	1 cut(s)	1839
AlwI	GGATC	5 cut(s)	251, 1389, 1916, 2196, 2234
AlwNI	CAGNNNCTG	5 cut(s)	159, 233, 902, 1508, 1694
AoxI	GGCC	2 cut(s)	148, 725
ApaLI	GTGCAC	1 cut(s)	1839
ApeKI	GCWGC	8 cut(s)	153, 212, 587, 798, 1028, 1442, 1637, 1973
ApoI	RAATTY	3 cut(s)	595, 879, 1020
AseI	ATTAAT	1 cut(s)	1334
Asp700I	GAANNNNTTC	3 cut(s)	275, 1416, 1784
AspS9I	GGNCC	2 cut(s)	34, 191
AsuC2I	CCSGG	1 cut(s)	32
AsuHPI	GGTGA	3 cut(s)	1369, 1692, 1823
AsuII	TTCGAA	1 cut(s)	1563
AvaII	GGWCC	2 cut(s)	34, 191
AxyI	CCTNAGG	1 cut(s)	1488
BaeGI	GKGCMC	2 cut(s)	923, 1843
BalI	TGGCCA	1 cut(s)	727
BanII	GRGCYC	2 cut(s)	24, 2133
BarI	GAAGNNNNNNTAC	2 cut(s)	1445, 1477
BbsI	GAAGAC	1 cut(s)	278
Bbv12I	GWGCWC	1 cut(s)	1843
BbvI	GCAGC	8 cut(s)	165, 199, 599, 810, 1015, 1429, 1624, 1985
BccI	CCATC	5 cut(s)	488, 1008, 1322, 1605, 2058
BceAI	ACGGC	3 cut(s)	57, 64, 163
BcgI	CGANNNNNNTGC	4 cut(s)	2171, 2184, 2205, 2218
BciT130I	CCWGG	4 cut(s)	184, 381, 729, 1720
BclI	TGATCA	1 cut(s)	1987
BcnI	CCSGG	1 cut(s)	32
BcoDI	GTCTC	6 cut(s)	145, 233, 454, 494, 920, 1766
BfaI	CTAG	7 cut(s)	134, 450, 842, 912, 1596, 1655, 2124
BfmI	CTRYAG	2 cut(s)	1638, 2142
BlpI	GCTNAGC	1 cut(s)	101
Bme1390I	CCNGG	5 cut(s)	32, 184, 381, 729, 1720
Bme18I	GGWCC	2 cut(s)	34, 191
BmgT120I	GGNCC	2 cut(s)	34, 191
BmiI	GGNNCC	3 cut(s)	35, 192, 193
BmrFI	CCNGG	5 cut(s)	32, 184, 381, 729, 1720
BmrI	ACTGGG	3 cut(s)	409, 994, 1597
BmsI	GCATC	6 cut(s)	1045, 1100, 1261, 1807, 2110, 2192
BmuI	ACTGGG	3 cut(s)	409, 994, 1597
BpiI	GAAGAC	1 cut(s)	278
BpmI	CTGGAG	1 cut(s)	1726
Bpu10I	CCTNAGC	3 cut(s)	1275, 1543, 2039
Bpu1102I	GCTNAGC	1 cut(s)	101
Bpu14I	TTCGAA	1 cut(s)	1563
BpuEI	CTTGAG	3 cut(s)	269, 590, 1689
BpuMI	CCSGG	1 cut(s)	32
Bsa29I	ATCGAT	1 cut(s)	2229
BsaAI	YACGTR	2 cut(s)	837, 1182
BsaHI	GRCGYC	1 cut(s)	1794
BsaI	GGTCTC	3 cut(s)	233, 454, 1766
BsaJI	CCNNGG	4 cut(s)	182, 1576, 1718, 1719
BsaXI	ACNNNNNCTCC	2 cut(s)	1806, 1836
Bsc4I	CCNNNNNNNGG	5 cut(s)	30, 31, 183, 188, 200
Bse1I	ACTGG	4 cut(s)	404, 625, 989, 1592
Bse21I	CCTNAGG	1 cut(s)	1488
Bse3DI	GCAATG	1 cut(s)	1497
BseBI	CCWGG	4 cut(s)	184, 381, 729, 1720
BseCI	ATCGAT	1 cut(s)	2229
BseDI	CCNNGG	4 cut(s)	182, 1576, 1718, 1719
BseGI	GGATG	7 cut(s)	88, 616, 1036, 1115, 1212, 1903, 2069
BseLI	CCNNNNNNNGG	5 cut(s)	30, 31, 183, 188, 200
BseMI	GCAATG	1 cut(s)	1497
BseMII	CTCAG	5 cut(s)	115, 915, 1289, 1479, 1557
BseNI	ACTGG	4 cut(s)	404, 625, 989, 1592
BseRI	GAGGAG	1 cut(s)	17
BseSI	GKGCMC	2 cut(s)	923, 1843
BseX3I	CGGCCG	1 cut(s)	148
BseXI	GCAGC	8 cut(s)	165, 199, 599, 810, 1015, 1429, 1624, 1985
BseYI	CCCAGC	1 cut(s)	1113
BsgI	GTGCAG	1 cut(s)	1926
Bsh1285I	CGRYCG	1 cut(s)	151
BshFI	GGCC	2 cut(s)	150, 727
BshVI	ATCGAT	1 cut(s)	2229
BsiEI	CGRYCG	1 cut(s)	151
BsiHKAI	GWGCWC	1 cut(s)	1843
BsiSI	CCGG	1 cut(s)	31
BslFI	GGGAC	5 cut(s)	47, 177, 1131, 1561, 2123
BslI	CCNNNNNNNGG	5 cut(s)	30, 31, 183, 188, 200
BsmAI	GTCTC	6 cut(s)	145, 233, 454, 494, 920, 1766
BsmFI	GGGAC	5 cut(s)	47, 177, 1131, 1561, 2123
BsnI	GGCC	2 cut(s)	150, 727
Bso31I	GGTCTC	3 cut(s)	233, 454, 1766
Bsp119I	TTCGAA	1 cut(s)	1563
Bsp1286I	GDGCHC	4 cut(s)	24, 923, 1843, 2133
Bsp1720I	GCTNAGC	1 cut(s)	101
BspACI	CCGC	8 cut(s)	70, 77, 151, 252, 289, 801, 1034, 1075
BspANI	GGCC	2 cut(s)	150, 727
BspCNI	CTCAG	5 cut(s)	114, 916, 1288, 1480, 1556
BspDI	ATCGAT	1 cut(s)	2229
BspLI	GGNNCC	3 cut(s)	35, 192, 193
BspMAI	CTGCAG	1 cut(s)	1642
BspPI	GGATC	5 cut(s)	251, 1389, 1916, 2196, 2234
BspQI	GCTCTTC	1 cut(s)	112
BspT104I	TTCGAA	1 cut(s)	1563
BspTNI	GGTCTC	3 cut(s)	233, 454, 1766
BsrDI	GCAATG	1 cut(s)	1497
BsrI	ACTGG	4 cut(s)	404, 625, 989, 1592
BssECI	CCNNGG	4 cut(s)	182, 1576, 1718, 1719
BssNI	GRCGYC	1 cut(s)	1794
BssT1I	CCWWGG	1 cut(s)	1576
Bst2UI	CCWGG	4 cut(s)	184, 381, 729, 1720
Bst6I	CTCTTC	1 cut(s)	112
BstACI	GRCGYC	1 cut(s)	1794
BstAPI	GCANNNNNTGC	1 cut(s)	1913
BstBAI	YACGTR	2 cut(s)	837, 1182
BstBI	TTCGAA	1 cut(s)	1563
BstC8I	GCNNGC	1 cut(s)	2036
BstDEI	CTNAG	8 cut(s)	101, 924, 933, 1275, 1488, 1543, 2039, 2165
BstF5I	GGATG	7 cut(s)	88, 616, 1036, 1115, 1212, 1903, 2069
BstMAI	GTCTC	6 cut(s)	145, 233, 454, 494, 920, 1766
BstMCI	CGRYCG	1 cut(s)	151
BstMWI	GCNNNNNNNGC	4 cut(s)	587, 1412, 1448, 1913
BstNI	CCWGG	4 cut(s)	184, 381, 729, 1720
BstNSI	RCATGY	2 cut(s)	724, 1717
BstSCI	CCNGG	5 cut(s)	30, 182, 379, 727, 1718
BstSFI	CTRYAG	2 cut(s)	1638, 2142
BstSLI	GKGCMC	2 cut(s)	923, 1843
BstV1I	GCAGC	8 cut(s)	165, 199, 599, 810, 1015, 1429, 1624, 1985
BstV2I	GAAGAC	1 cut(s)	278
BstX2I	RGATCY	1 cut(s)	1394
BstYI	RGATCY	1 cut(s)	1394
BstZI	CGGCCG	1 cut(s)	148
Bsu15I	ATCGAT	1 cut(s)	2229
Bsu36I	CCTNAGG	1 cut(s)	1488
BsuRI	GGCC	2 cut(s)	150, 727
BsuTUI	ATCGAT	1 cut(s)	2229
BtsCI	GGATG	7 cut(s)	88, 616, 1036, 1115, 1212, 1903, 2069
BtsI	GCAGTG	2 cut(s)	114, 811
BtsIMutI	CAGTG	7 cut(s)	114, 231, 397, 583, 811, 982, 1662
Cac8I	GCNNGC	1 cut(s)	2036
CaiI	CAGNNNCTG	5 cut(s)	159, 233, 902, 1508, 1694
Cfr13I	GGNCC	2 cut(s)	34, 191
ClaI	ATCGAT	1 cut(s)	2229
Csp6I	GTAC	5 cut(s)	1179, 1293, 1615, 1711, 2056
CspCI	CAANNNNNGTGG	2 cut(s)	1936, 1971
CviAII	CATG	6 cut(s)	332, 721, 1618, 1714, 1790, 1970
CviQI	GTAC	5 cut(s)	1179, 1293, 1615, 1711, 2056
DdeI	CTNAG	8 cut(s)	101, 924, 933, 1275, 1488, 1543, 2039, 2165
DraI	TTTAAA	1 cut(s)	1890
DrdI	GACNNNNNNGTC	1 cut(s)	72
DseDI	GACNNNNNNGTC	1 cut(s)	72
EaeI	YGGCCR	2 cut(s)	148, 725
EagI	CGGCCG	1 cut(s)	148
Eam1104I	CTCTTC	1 cut(s)	112
EarI	CTCTTC	1 cut(s)	112
EciI	GGCGGA	2 cut(s)	66, 241
EclXI	CGGCCG	1 cut(s)	148
Eco130I	CCWWGG	1 cut(s)	1576
Eco24I	GRGCYC	2 cut(s)	24, 2133
Eco31I	GGTCTC	3 cut(s)	233, 454, 1766
Eco32I	GATATC	1 cut(s)	2017
Eco47I	GGWCC	2 cut(s)	34, 191
Eco52I	CGGCCG	1 cut(s)	148
Eco57I	CTGAAG	5 cut(s)	1287, 1447, 1467, 1530, 1787
Eco81I	CCTNAGG	1 cut(s)	1488
EcoO109I	RGGNCCY	2 cut(s)	34, 191
EcoRII	CCWGG	4 cut(s)	182, 379, 727, 1718
EcoRV	GATATC	1 cut(s)	2017
EcoT14I	CCWWGG	1 cut(s)	1576
EcoT38I	GRGCYC	2 cut(s)	24, 2133
ErhI	CCWWGG	1 cut(s)	1576
FaeI	CATG	6 cut(s)	335, 724, 1621, 1717, 1793, 1973
FalI	AAGNNNNNCTT	4 cut(s)	260, 292, 1404, 1436
FaqI	GGGAC	5 cut(s)	47, 177, 1131, 1561, 2123
FatI	CATG	6 cut(s)	331, 720, 1617, 1713, 1789, 1969
FauI	CCCGC	1 cut(s)	1041
FauNDI	CATATG	1 cut(s)	1321
FbaI	TGATCA	1 cut(s)	1987
FblI	GTMKAC	2 cut(s)	5, 52
FokI	GGATG	7 cut(s)	75, 623, 1023, 1122, 1199, 1910, 2076
FriOI	GRGCYC	2 cut(s)	24, 2133
FspBI	CTAG	7 cut(s)	134, 450, 842, 912, 1596, 1655, 2124
GsaI	CCCAGC	1 cut(s)	1117
GsuI	CTGGAG	1 cut(s)	1726
HaeIII	GGCC	2 cut(s)	150, 727
HapII	CCGG	1 cut(s)	31
Hin1I	GRCGYC	1 cut(s)	1794
Hin1II	CATG	6 cut(s)	335, 724, 1621, 1717, 1793, 1973
HincII	GTYRAC	2 cut(s)	6, 53
HindII	GTYRAC	2 cut(s)	6, 53
HpaII	CCGG	1 cut(s)	31
HphI	GGTGA	3 cut(s)	1369, 1692, 1823
Hpy166II	GTNNAC	8 cut(s)	6, 53, 418, 743, 889, 982, 1125, 1841
Hpy188III	TCNNGA	9 cut(s)	238, 791, 813, 842, 1424, 1605, 1743, 1798, 2199
Hpy8I	GTNNAC	8 cut(s)	6, 53, 418, 743, 889, 982, 1125, 1841
Hpy99I	CGWCG	6 cut(s)	10, 54, 85, 1799, 2063, 2196
HpyCH4IV	ACGT	5 cut(s)	753, 836, 1181, 1365, 1794
HpyF10VI	GCNNNNNNNGC	4 cut(s)	587, 1412, 1448, 1913
HpyF3I	CTNAG	8 cut(s)	101, 924, 933, 1275, 1488, 1543, 2039, 2165
HpySE526I	ACGT	5 cut(s)	753, 836, 1181, 1365, 1794
Hsp92I	GRCGYC	1 cut(s)	1794
Hsp92II	CATG	6 cut(s)	335, 724, 1621, 1717, 1793, 1973
KflI	GGGWCCC	1 cut(s)	191
Ksp22I	TGATCA	1 cut(s)	1987
LguI	GCTCTTC	1 cut(s)	112
Lsp1109I	GCAGC	8 cut(s)	165, 199, 599, 810, 1015, 1429, 1624, 1985
LweI	GCATC	6 cut(s)	1045, 1100, 1261, 1807, 2110, 2192
MaeI	CTAG	7 cut(s)	134, 450, 842, 912, 1596, 1655, 2124
MaeII	ACGT	5 cut(s)	753, 836, 1181, 1365, 1794
MaeIII	GTNAC	5 cut(s)	161, 647, 898, 1361, 1943
MfeI	CAATTG	2 cut(s)	582, 867
MflI	RGATCY	1 cut(s)	1394
MhlI	GDGCHC	4 cut(s)	24, 923, 1843, 2133
MlsI	TGGCCA	1 cut(s)	727
MluNI	TGGCCA	1 cut(s)	727
MlyI	GAGTC	4 cut(s)	213, 1072, 1134, 1700
MmeI	TCCRAC	5 cut(s)	168, 679, 774, 1502, 2204
Mox20I	TGGCCA	1 cut(s)	727
MroXI	GAANNNNTTC	3 cut(s)	275, 1416, 1784
MscI	TGGCCA	1 cut(s)	727
MseI	TTAA	5 cut(s)	95, 567, 1334, 1584, 1889
MslI	CAYNNNNRTG	1 cut(s)	1836
Msp20I	TGGCCA	1 cut(s)	727
MspA1I	CMGCKG	3 cut(s)	467, 801, 1028
MspI	CCGG	1 cut(s)	31
MspR9I	CCNGG	5 cut(s)	32, 184, 381, 729, 1720
MunI	CAATTG	2 cut(s)	582, 867
MvaI	CCWGG	4 cut(s)	184, 381, 729, 1720
MwoI	GCNNNNNNNGC	4 cut(s)	587, 1412, 1448, 1913
NciI	CCSGG	1 cut(s)	32
NdeI	CATATG	1 cut(s)	1321
NlaIII	CATG	6 cut(s)	335, 724, 1621, 1717, 1793, 1973
NlaIV	GGNNCC	3 cut(s)	35, 192, 193
NmuCI	GTSAC	2 cut(s)	647, 1361
NspI	RCATGY	2 cut(s)	724, 1717
NspV	TTCGAA	1 cut(s)	1563
OliI	CACNNNNGTG	1 cut(s)	1836
PasI	CCCWGGG	1 cut(s)	1719
PciSI	GCTCTTC	1 cut(s)	112
PcsI	WCGNNNNNNNCGW	1 cut(s)	243
PdmI	GAANNNNTTC	3 cut(s)	275, 1416, 1784
PfoI	TCCNGGA	2 cut(s)	30, 379
PleI	GAGTC	4 cut(s)	212, 1072, 1134, 1699
PpsI	GAGTC	4 cut(s)	212, 1072, 1134, 1699
Ppu21I	YACGTR	2 cut(s)	837, 1182
PpuMI	RGGWCCY	2 cut(s)	34, 191
PshBI	ATTAAT	1 cut(s)	1334
Psp5II	RGGWCCY	2 cut(s)	34, 191
Psp6I	CCWGG	4 cut(s)	182, 379, 727, 1718
PspFI	CCCAGC	1 cut(s)	1113
PspGI	CCWGG	4 cut(s)	182, 379, 727, 1718
PspN4I	GGNNCC	3 cut(s)	35, 192, 193
PspPI	GGNCC	2 cut(s)	34, 191
PspPPI	RGGWCCY	2 cut(s)	34, 191
PsrI	GAACNNNNNNTAC	2 cut(s)	1730, 1762
PstI	CTGCAG	1 cut(s)	1642
PstNI	CAGNNNCTG	5 cut(s)	159, 233, 902, 1508, 1694
PsuI	RGATCY	1 cut(s)	1394
PvuII	CAGCTG	2 cut(s)	467, 1028
RsaI	GTAC	5 cut(s)	1180, 1294, 1616, 1712, 2057
RsaNI	GTAC	5 cut(s)	1179, 1293, 1615, 1711, 2056
RseI	CAYNNNNRTG	1 cut(s)	1836
SalI	GTCGAC	2 cut(s)	4, 51
SapI	GCTCTTC	1 cut(s)	112
SaqAI	TTAA	5 cut(s)	95, 567, 1334, 1584, 1889
Sau96I	GGNCC	2 cut(s)	34, 191
SchI	GAGTC	4 cut(s)	213, 1072, 1134, 1700
ScrFI	CCNGG	5 cut(s)	32, 184, 381, 729, 1720
SduI	GDGCHC	4 cut(s)	24, 923, 1843, 2133
SfaNI	GCATC	6 cut(s)	1045, 1100, 1261, 1807, 2110, 2192
SfcI	CTRYAG	2 cut(s)	1638, 2142
SfuI	TTCGAA	1 cut(s)	1563
SinI	GGWCC	2 cut(s)	34, 191
SmiMI	CAYNNNNRTG	1 cut(s)	1836
SmlI	CTYRAG	3 cut(s)	284, 605, 1704
SmoI	CTYRAG	3 cut(s)	284, 605, 1704
SsiI	CCGC	8 cut(s)	70, 77, 151, 252, 289, 801, 1034, 1075
SspI	AATATT	1 cut(s)	1569
SspMI	CTAG	7 cut(s)	134, 450, 842, 912, 1596, 1655, 2124
StyD4I	CCNGG	5 cut(s)	30, 182, 379, 727, 1718
StyI	CCWWGG	1 cut(s)	1576
TaiI	ACGT	5 cut(s)	756, 839, 1184, 1368, 1797
TaqI	TCGA	8 cut(s)	5, 52, 83, 237, 1081, 1563, 2191, 2229
TatI	WGTACW	1 cut(s)	1292
TauI	GCSGC	2 cut(s)	153, 804
Tru1I	TTAA	5 cut(s)	95, 567, 1334, 1584, 1889
Tru9I	TTAA	5 cut(s)	95, 567, 1334, 1584, 1889
TscAI	CASTG	7 cut(s)	121, 238, 404, 583, 811, 989, 1669
TseFI	GTSAC	2 cut(s)	647, 1361
TseI	GCWGC	8 cut(s)	153, 212, 587, 798, 1028, 1442, 1637, 1973
Tsp45I	GTSAC	2 cut(s)	647, 1361
TspDTI	ATGAA	7 cut(s)	357, 564, 627, 870, 1188, 1356, 1958
TspGWI	ACGGA	4 cut(s)	308, 1302, 1764, 2076
TspRI	CASTG	7 cut(s)	121, 238, 404, 583, 811, 989, 1669
VneI	GTGCAC	1 cut(s)	1839
VpaK11BI	GGWCC	2 cut(s)	34, 191
VspI	ATTAAT	1 cut(s)	1334
XapI	RAATTY	3 cut(s)	595, 879, 1020
XbaI	TCTAGA	1 cut(s)	841
XceI	RCATGY	2 cut(s)	724, 1717
XmiI	GTMKAC	2 cut(s)	5, 52
XmnI	GAANNNNTTC	3 cut(s)	275, 1416, 1784
XspI	CTAG	7 cut(s)	134, 450, 842, 912, 1596, 1655, 2124
ZraI	GACGTC	1 cut(s)	1795

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.