Rorug05G0356300

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0000937
Physical Location & Seq
Reverse (-)
44888141 .. 44914702
26562 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0356300.1

Sequence Viewer

Length: 564 bp
ATGGCTAGCCCCGTCTATGACATGCATCCGGTAGGCCGACACCCGAGCTACGTTGCCATGGTGGAGGTCGGCCGGTATCTAGTCGGGAGGCCACCCTCCCATGCTCTACAAGAGGCTTCAAGGGAAGCAATATATTATCTTAACAAGGCCCGGTGGAAGAAGAAGAGATTGTGCAGATCAACAATGAAGAAATCGATCATCAAGCCAAATCAAGGACATCAAAAAGAAGAAAACAAGGTCAGTATATATATATATATAGTATCTCAACCAATAATGACAAGACAGAATCAGCTTGGGGAAATGAGCAATCAGAATGTTGACATAATGGAAGATCGTGATCAACAAGAGGTTGGTCAGATCACAGATGAATATCAACATTCATACGGTCAAGCATCATATCAAAGAAGTAGACAAGGATCCGCTATTGCATATGAAGATTCAGGAATTAGCAACAGTGAGACCTCGGTGAGAGCTCAGCTTTTTGAAATCAATATCAACACAAGAGTTACAACATTTGCTAAAGAAAAGTTCTCCAGGTATCTTATTCAAGTTGTAAACGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

187

Amino Acids

21.58

Weight (kDa)

9.36

Isoelectric Point (pI)

50.79

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 409
AciI CCGC 1 cut(s) 420
AclI AACGTT 1 cut(s) 558
AclWI GGATC 2 cut(s) 411, 424
AcoI YGGCCR 1 cut(s) 70
AfiI CCNNNNNNNGG 1 cut(s) 212
AgsI TTSAA 3 cut(s) 120, 485, 548
AjnI CCWGG 1 cut(s) 533
AluBI AGCT 4 cut(s) 48, 292, 473, 478
AluI AGCT 4 cut(s) 48, 292, 473, 478
Alw21I GWGCWC 1 cut(s) 475
Alw26I GTCTC 1 cut(s) 452
AlwI GGATC 2 cut(s) 411, 424
Ama87I CYCGRG 1 cut(s) 43
AoxI GGCC 4 cut(s) 34, 70, 89, 147
AspS9I GGNCC 1 cut(s) 148
AsuC2I CCSGG 1 cut(s) 151
AsuHPI GGTGA 1 cut(s) 478
AsuNHI GCTAGC 1 cut(s) 5
AvaI CYCGRG 1 cut(s) 43
BamHI GGATCC 1 cut(s) 416
BanII GRGCYC 1 cut(s) 475
Bbv12I GWGCWC 1 cut(s) 475
BciT130I CCWGG 1 cut(s) 535
BclI TGATCA 1 cut(s) 337
BcnI CCSGG 1 cut(s) 151
BcoDI GTCTC 1 cut(s) 452
BfaI CTAG 2 cut(s) 6, 80
BlpI GCTNAGC 1 cut(s) 474
Bme1390I CCNGG 2 cut(s) 151, 535
BmeT110I CYCGRG 1 cut(s) 43
BmgT120I GGNCC 1 cut(s) 148
BmiI GGNNCC 1 cut(s) 418
BmrFI CCNGG 2 cut(s) 151, 535
BmsI GCATC 2 cut(s) 34, 401
BmtI GCTAGC 1 cut(s) 9
BpmI CTGGAG 1 cut(s) 517
Bpu1102I GCTNAGC 1 cut(s) 474
BpuMI CCSGG 1 cut(s) 151
Bsa29I ATCGAT 1 cut(s) 194
BsaBI GATNNNNATC 2 cut(s) 336, 369
BsaI GGTCTC 1 cut(s) 452
BsaJI CCNNGG 2 cut(s) 57, 462
BsaWI WCCGGW 1 cut(s) 28
Bsc4I CCNNNNNNNGG 1 cut(s) 212
Bse118I RCCGGY 1 cut(s) 72
Bse8I GATNNNNATC 2 cut(s) 336, 369
BseBI CCWGG 1 cut(s) 535
BseCI ATCGAT 1 cut(s) 194
BseDI CCNNGG 2 cut(s) 57, 462
BseGI GGATG 1 cut(s) 25
BseJI GATNNNNATC 2 cut(s) 336, 369
BseLI CCNNNNNNNGG 1 cut(s) 212
BseMII CTCAG 1 cut(s) 488
BseX3I CGGCCG 1 cut(s) 70
BsgI GTGCAG 1 cut(s) 193
Bsh1285I CGRYCG 1 cut(s) 73
BshFI GGCC 4 cut(s) 36, 72, 91, 149
BshVI ATCGAT 1 cut(s) 194
BsiEI CGRYCG 1 cut(s) 73
BsiHKAI GWGCWC 1 cut(s) 475
BsiHKCI CYCGRG 1 cut(s) 43
BsiSI CCGG 3 cut(s) 29, 73, 151
BslI CCNNNNNNNGG 1 cut(s) 212
BsmAI GTCTC 1 cut(s) 452
BsnI GGCC 4 cut(s) 36, 72, 91, 149
Bso31I GGTCTC 1 cut(s) 452
BsoBI CYCGRG 1 cut(s) 43
Bsp1286I GDGCHC 1 cut(s) 475
Bsp143I GATC 6 cut(s) 176, 195, 331, 337, 357, 416
Bsp1720I GCTNAGC 1 cut(s) 474
Bsp19I CCATGG 1 cut(s) 57
BspACI CCGC 1 cut(s) 420
BspANI GGCC 4 cut(s) 36, 72, 91, 149
BspCNI CTCAG 1 cut(s) 487
BspDI ATCGAT 1 cut(s) 194
BspLI GGNNCC 1 cut(s) 418
BspOI GCTAGC 1 cut(s) 9
BspPI GGATC 2 cut(s) 411, 424
BspTNI GGTCTC 1 cut(s) 452
BsrFI RCCGGY 1 cut(s) 72
BssAI RCCGGY 1 cut(s) 72
BssECI CCNNGG 2 cut(s) 57, 462
BssMI GATC 6 cut(s) 176, 195, 331, 337, 357, 416
BssT1I CCWWGG 1 cut(s) 57
Bst2UI CCWGG 1 cut(s) 535
Bst4CI ACNGT 2 cut(s) 386, 455
Bst6I CTCTTC 1 cut(s) 158
BstC8I GCNNGC 1 cut(s) 7
BstDEI CTNAG 1 cut(s) 474
BstDSI CCRYGG 1 cut(s) 57
BstF5I GGATG 1 cut(s) 25
BstKTI GATC 6 cut(s) 179, 198, 334, 340, 360, 419
BstMAI GTCTC 1 cut(s) 452
BstMBI GATC 6 cut(s) 176, 195, 331, 337, 357, 416
BstMCI CGRYCG 1 cut(s) 73
BstNI CCWGG 1 cut(s) 535
BstNSI RCATGY 1 cut(s) 25
BstSCI CCNGG 2 cut(s) 149, 533
BstX2I RGATCY 1 cut(s) 416
BstYI RGATCY 1 cut(s) 416
BstZI CGGCCG 1 cut(s) 70
Bsu15I ATCGAT 1 cut(s) 194
BsuRI GGCC 4 cut(s) 36, 72, 91, 149
BsuTUI ATCGAT 1 cut(s) 194
BtgI CCRYGG 1 cut(s) 57
BtsCI GGATG 1 cut(s) 25
BtsIMutI CAGTG 1 cut(s) 460
Cac8I GCNNGC 1 cut(s) 7
Cfr10I RCCGGY 1 cut(s) 72
Cfr13I GGNCC 1 cut(s) 148
ClaI ATCGAT 1 cut(s) 194
CviAII CATG 3 cut(s) 22, 58, 101
DdeI CTNAG 1 cut(s) 474
DpnI GATC 6 cut(s) 178, 197, 333, 339, 359, 418
DpnII GATC 6 cut(s) 176, 195, 331, 337, 357, 416
EaeI YGGCCR 1 cut(s) 70
EagI CGGCCG 1 cut(s) 70
Eam1104I CTCTTC 1 cut(s) 158
EarI CTCTTC 1 cut(s) 158
Ecl136II GAGCTC 1 cut(s) 473
EclXI CGGCCG 1 cut(s) 70
Eco130I CCWWGG 1 cut(s) 57
Eco24I GRGCYC 1 cut(s) 475
Eco31I GGTCTC 1 cut(s) 452
Eco52I CGGCCG 1 cut(s) 70
Eco53kI GAGCTC 1 cut(s) 473
Eco88I CYCGRG 1 cut(s) 43
EcoICRI GAGCTC 1 cut(s) 473
EcoRII CCWGG 1 cut(s) 533
EcoT14I CCWWGG 1 cut(s) 57
EcoT22I ATGCAT 1 cut(s) 27
EcoT38I GRGCYC 1 cut(s) 475
ErhI CCWWGG 1 cut(s) 57
FaeI CATG 3 cut(s) 25, 61, 104
FatI CATG 3 cut(s) 21, 57, 100
FauNDI CATATG 1 cut(s) 430
FbaI TGATCA 1 cut(s) 337
FblI GTMKAC 1 cut(s) 409
FokI GGATG 1 cut(s) 12
FriOI GRGCYC 1 cut(s) 475
FspBI CTAG 2 cut(s) 6, 80
GsuI CTGGAG 1 cut(s) 517
HaeIII GGCC 4 cut(s) 36, 72, 91, 149
HapII CCGG 3 cut(s) 29, 73, 151
Hin1II CATG 3 cut(s) 25, 61, 104
HincII GTYRAC 1 cut(s) 319
HindII GTYRAC 1 cut(s) 319
HinfI GANTC 2 cut(s) 286, 437
HpaII CCGG 3 cut(s) 29, 73, 151
HphI GGTGA 1 cut(s) 478
Hpy166II GTNNAC 3 cut(s) 319, 410, 556
Hpy188I TCNGA 2 cut(s) 312, 357
Hpy188III TCNNGA 3 cut(s) 85, 335, 441
Hpy8I GTNNAC 3 cut(s) 319, 410, 556
HpyCH4III ACNGT 2 cut(s) 386, 455
HpyCH4IV ACGT 2 cut(s) 51, 558
HpyCH4V TGCA 3 cut(s) 25, 174, 428
HpyF3I CTNAG 1 cut(s) 474
HpySE526I ACGT 2 cut(s) 51, 558
Hsp92II CATG 3 cut(s) 25, 61, 104
Ksp22I TGATCA 1 cut(s) 337
Kzo9I GATC 6 cut(s) 176, 195, 331, 337, 357, 416
LpnPI CCDG 6 cut(s) 42, 86, 164, 426, 520, 547
LweI GCATC 2 cut(s) 34, 401
MaeI CTAG 2 cut(s) 6, 80
MaeII ACGT 2 cut(s) 51, 558
MaeIII GTNAC 1 cut(s) 505
MalI GATC 6 cut(s) 178, 197, 333, 339, 359, 418
MboI GATC 6 cut(s) 176, 195, 331, 337, 357, 416
MboII GAAGA 7 cut(s) 169, 172, 175, 199, 239, 341, 446
MflI RGATCY 1 cut(s) 416
MhlI GDGCHC 1 cut(s) 475
MluCI AATT 1 cut(s) 444
MnlI CCTC 6 cut(s) 58, 81, 106, 106, 340, 472
Mph1103I ATGCAT 1 cut(s) 27
MseI TTAA 1 cut(s) 141
MspI CCGG 3 cut(s) 29, 73, 151
MspR9I CCNGG 2 cut(s) 151, 535
MvaI CCWGG 1 cut(s) 535
NciI CCSGG 1 cut(s) 151
NcoI CCATGG 1 cut(s) 57
NdeI CATATG 1 cut(s) 430
NdeII GATC 6 cut(s) 176, 195, 331, 337, 357, 416
NheI GCTAGC 1 cut(s) 5
NlaIII CATG 3 cut(s) 25, 61, 104
NlaIV GGNNCC 1 cut(s) 418
NsiI ATGCAT 1 cut(s) 27
NspI RCATGY 1 cut(s) 25
PfeI GAWTC 2 cut(s) 286, 437
Psp124BI GAGCTC 1 cut(s) 475
Psp1406I AACGTT 1 cut(s) 558
Psp6I CCWGG 1 cut(s) 533
PspGI CCWGG 1 cut(s) 533
PspN4I GGNNCC 1 cut(s) 418
PspPI GGNCC 1 cut(s) 148
PsuI RGATCY 1 cut(s) 416
SacI GAGCTC 1 cut(s) 475
SaqAI TTAA 1 cut(s) 141
Sau3AI GATC 6 cut(s) 176, 195, 331, 337, 357, 416
Sau96I GGNCC 1 cut(s) 148
ScrFI CCNGG 2 cut(s) 151, 535
SduI GDGCHC 1 cut(s) 475
SfaNI GCATC 2 cut(s) 34, 401
Sse9I AATT 1 cut(s) 444
SsiI CCGC 1 cut(s) 420
SspMI CTAG 2 cut(s) 6, 80
SstI GAGCTC 1 cut(s) 475
StyD4I CCNGG 2 cut(s) 149, 533
StyI CCWWGG 1 cut(s) 57
TaaI ACNGT 2 cut(s) 386, 455
TaiI ACGT 2 cut(s) 54, 561
TaqI TCGA 1 cut(s) 194
TasI AATT 1 cut(s) 444
TfiI GAWTC 2 cut(s) 286, 437
Tru1I TTAA 1 cut(s) 141
Tru9I TTAA 1 cut(s) 141
TscAI CASTG 1 cut(s) 460
TspDTI ATGAA 4 cut(s) 200, 369, 381, 447
TspRI CASTG 1 cut(s) 460
XceI RCATGY 1 cut(s) 25
XmiI GTMKAC 1 cut(s) 409
XspI CTAG 2 cut(s) 6, 80
Zsp2I ATGCAT 1 cut(s) 27
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.