Cupin-like domain
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 2018 |
| AatII | GACGTC | 2 cut(s) | 2253, 2562 |
| AccB1I | GGYRCC | 1 cut(s) | 1126 |
| AccB7I | CCANNNNNTGG | 2 cut(s) | 810, 1479 |
| AccI | GTMKAC | 1 cut(s) | 2815 |
| AccII | CGCG | 1 cut(s) | 1305 |
| AccIII | TCCGGA | 2 cut(s) | 760, 2155 |
| AciI | CCGC | 5 cut(s) | 604, 828, 968, 1303, 1794 |
| AclWI | GGATC | 3 cut(s) | 608, 1825, 2519 |
| AcoI | YGGCCR | 3 cut(s) | 470, 1744, 2006 |
| AcsI | RAATTY | 4 cut(s) | 558, 1081, 1186, 1910 |
| AcuI | CTGAAG | 5 cut(s) | 1212, 1508, 1800, 2488, 2619 |
| AcyI | GRCGYC | 5 cut(s) | 429, 1127, 2250, 2482, 2559 |
| AfaI | GTAC | 6 cut(s) | 314, 1576, 1706, 1717, 2668, 2701 |
| AfiI | CCNNNNNNNGG | 6 cut(s) | 810, 967, 1309, 1479, 1841, 2397 |
| AflII | CTTAAG | 1 cut(s) | 2585 |
| AflIII | ACRYGT | 2 cut(s) | 1999, 2150 |
| AgeI | ACCGGT | 1 cut(s) | 2143 |
| AhlI | ACTAGT | 1 cut(s) | 2255 |
| AjiI | CACGTC | 2 cut(s) | 2153, 2353 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 1902, 1934 |
| AluBI | AGCT | 8 cut(s) | 650, 797, 1239, 1477, 1615, 1723, 1872, 2593 |
| AluI | AGCT | 8 cut(s) | 650, 797, 1239, 1477, 1615, 1723, 1872, 2593 |
| Alw21I | GWGCWC | 1 cut(s) | 995 |
| Alw26I | GTCTC | 7 cut(s) | 53, 62, 188, 1286, 2357, 2565, 2691 |
| Alw44I | GTGCAC | 1 cut(s) | 991 |
| AlwI | GGATC | 3 cut(s) | 608, 1825, 2519 |
| AlwNI | CAGNNNCTG | 3 cut(s) | 810, 1070, 2089 |
| Ama87I | CYCGRG | 2 cut(s) | 102, 2289 |
| Aor13HI | TCCGGA | 2 cut(s) | 760, 2155 |
| AoxI | GGCC | 5 cut(s) | 454, 470, 1444, 1744, 2006 |
| ApaLI | GTGCAC | 1 cut(s) | 991 |
| ApeKI | GCWGC | 2 cut(s) | 650, 1632 |
| ApoI | RAATTY | 4 cut(s) | 558, 1081, 1186, 1910 |
| AseI | ATTAAT | 1 cut(s) | 2618 |
| AsiGI | ACCGGT | 1 cut(s) | 2143 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 1614, 2319 |
| AspLEI | GCGC | 2 cut(s) | 809, 1129 |
| AspS9I | GGNCC | 2 cut(s) | 200, 758 |
| AsuC2I | CCSGG | 1 cut(s) | 1748 |
| AsuHPI | GGTGA | 3 cut(s) | 107, 530, 917 |
| AsuII | TTCGAA | 1 cut(s) | 1809 |
| AvaI | CYCGRG | 2 cut(s) | 102, 2289 |
| AvaII | GGWCC | 2 cut(s) | 200, 758 |
| BaeGI | GKGCMC | 2 cut(s) | 684, 995 |
| BalI | TGGCCA | 2 cut(s) | 472, 2008 |
| BanI | GGYRCC | 1 cut(s) | 1126 |
| BanII | GRGCYC | 2 cut(s) | 551, 2296 |
| BbsI | GAAGAC | 2 cut(s) | 981, 2347 |
| Bbv12I | GWGCWC | 1 cut(s) | 995 |
| BbvI | GCAGC | 2 cut(s) | 637, 1644 |
| BccI | CCATC | 9 cut(s) | 80, 290, 738, 896, 1146, 1835, 2456, 2537, 2698 |
| BceAI | ACGGC | 2 cut(s) | 1140, 1576 |
| BcgI | CGANNNNNNTGC | 4 cut(s) | 77, 111, 1871, 1905 |
| BciVI | GTATCC | 3 cut(s) | 861, 1055, 2394 |
| BcnI | CCSGG | 1 cut(s) | 1748 |
| BcoDI | GTCTC | 7 cut(s) | 53, 62, 188, 1286, 2357, 2565, 2691 |
| BcuI | ACTAGT | 1 cut(s) | 2255 |
| BfaI | CTAG | 9 cut(s) | 245, 872, 947, 1562, 1691, 1787, 1869, 2256, 2597 |
| BfmI | CTRYAG | 4 cut(s) | 331, 673, 935, 1440 |
| BfoI | RGCGCY | 2 cut(s) | 810, 1130 |
| BfrI | CTTAAG | 1 cut(s) | 2585 |
| BfuI | GTATCC | 3 cut(s) | 861, 1055, 2394 |
| BglII | AGATCT | 2 cut(s) | 711, 2630 |
| BisI | GCNGC | 2 cut(s) | 651, 1633 |
| BlsI | GCNGC | 2 cut(s) | 652, 1634 |
| Bme18I | GGWCC | 2 cut(s) | 200, 758 |
| BmeT110I | CYCGRG | 2 cut(s) | 102, 2289 |
| BmgBI | CACGTC | 2 cut(s) | 2153, 2353 |
| BmgT120I | GGNCC | 2 cut(s) | 200, 758 |
| BmiI | GGNNCC | 3 cut(s) | 548, 1128, 2439 |
| BmrI | ACTGGG | 2 cut(s) | 2281, 2481 |
| BmsI | GCATC | 8 cut(s) | 25, 250, 785, 1547, 1879, 1901, 2018, 2215 |
| BmuI | ACTGGG | 2 cut(s) | 2281, 2481 |
| BpiI | GAAGAC | 2 cut(s) | 981, 2347 |
| BpmI | CTGGAG | 2 cut(s) | 639, 1858 |
| Bpu14I | TTCGAA | 1 cut(s) | 1809 |
| BpuEI | CTTGAG | 2 cut(s) | 243, 1745 |
| BpuMI | CCSGG | 1 cut(s) | 1748 |
| Bsa29I | ATCGAT | 1 cut(s) | 1371 |
| BsaAI | YACGTR | 2 cut(s) | 486, 1943 |
| BsaBI | GATNNNNATC | 1 cut(s) | 612 |
| BsaHI | GRCGYC | 5 cut(s) | 429, 1127, 2250, 2482, 2559 |
| BsaI | GGTCTC | 1 cut(s) | 62 |
| BsaJI | CCNNGG | 6 cut(s) | 402, 856, 1421, 1835, 1957, 2288 |
| BsaWI | WCCGGW | 3 cut(s) | 760, 2143, 2155 |
| Bsc4I | CCNNNNNNNGG | 6 cut(s) | 810, 967, 1309, 1479, 1841, 2397 |
| Bse118I | RCCGGY | 1 cut(s) | 2143 |
| Bse1I | ACTGG | 6 cut(s) | 477, 1619, 1633, 1875, 2276, 2487 |
| Bse3DI | GCAATG | 2 cut(s) | 236, 1527 |
| Bse8I | GATNNNNATC | 1 cut(s) | 612 |
| BseAI | TCCGGA | 2 cut(s) | 760, 2155 |
| BseCI | ATCGAT | 1 cut(s) | 1371 |
| BseDI | CCNNGG | 6 cut(s) | 402, 856, 1421, 1835, 1957, 2288 |
| BseGI | GGATG | 9 cut(s) | 177, 412, 613, 1281, 1318, 1846, 1892, 2425, 2690 |
| BseJI | GATNNNNATC | 1 cut(s) | 612 |
| BseLI | CCNNNNNNNGG | 6 cut(s) | 810, 967, 1309, 1479, 1841, 2397 |
| BseMI | GCAATG | 2 cut(s) | 236, 1527 |
| BseNI | ACTGG | 6 cut(s) | 477, 1619, 1633, 1875, 2276, 2487 |
| BseRI | GAGGAG | 3 cut(s) | 559, 2201, 2583 |
| BseSI | GKGCMC | 2 cut(s) | 684, 995 |
| BseXI | GCAGC | 2 cut(s) | 637, 1644 |
| BseYI | CCCAGC | 2 cut(s) | 793, 1160 |
| BsgI | GTGCAG | 1 cut(s) | 2377 |
| Bsh1236I | CGCG | 1 cut(s) | 1305 |
| Bsh1285I | CGRYCG | 1 cut(s) | 832 |
| BshFI | GGCC | 5 cut(s) | 456, 472, 1446, 1746, 2008 |
| BshNI | GGYRCC | 1 cut(s) | 1126 |
| BshTI | ACCGGT | 1 cut(s) | 2143 |
| BshVI | ATCGAT | 1 cut(s) | 1371 |
| BsiEI | CGRYCG | 1 cut(s) | 832 |
| BsiHKAI | GWGCWC | 1 cut(s) | 995 |
| BsiHKCI | CYCGRG | 2 cut(s) | 102, 2289 |
| BsiSI | CCGG | 4 cut(s) | 761, 1747, 2144, 2156 |
| BslFI | GGGAC | 4 cut(s) | 509, 1817, 2469, 2774 |
| BslI | CCNNNNNNNGG | 6 cut(s) | 810, 967, 1309, 1479, 1841, 2397 |
| BsmAI | GTCTC | 7 cut(s) | 53, 62, 188, 1286, 2357, 2565, 2691 |
| BsmBI | CGTCTC | 1 cut(s) | 2565 |
| BsmFI | GGGAC | 4 cut(s) | 509, 1817, 2469, 2774 |
| BsmI | GAATGC | 1 cut(s) | 2179 |
| BsnI | GGCC | 5 cut(s) | 456, 472, 1446, 1746, 2008 |
| Bso31I | GGTCTC | 1 cut(s) | 62 |
| BsoBI | CYCGRG | 2 cut(s) | 102, 2289 |
| Bsp119I | TTCGAA | 1 cut(s) | 1809 |
| Bsp1286I | GDGCHC | 4 cut(s) | 551, 684, 995, 2296 |
| Bsp13I | TCCGGA | 2 cut(s) | 760, 2155 |
| Bsp19I | CCATGG | 1 cut(s) | 1421 |
| BspACI | CCGC | 5 cut(s) | 604, 828, 968, 1303, 1794 |
| BspANI | GGCC | 5 cut(s) | 456, 472, 1446, 1746, 2008 |
| BspDI | ATCGAT | 1 cut(s) | 1371 |
| BspEI | TCCGGA | 2 cut(s) | 760, 2155 |
| BspFNI | CGCG | 1 cut(s) | 1305 |
| BspHI | TCATGA | 1 cut(s) | 2182 |
| BspLI | GGNNCC | 3 cut(s) | 548, 1128, 2439 |
| BspMAI | CTGCAG | 1 cut(s) | 939 |
| BspPI | GGATC | 3 cut(s) | 608, 1825, 2519 |
| BspQI | GCTCTTC | 1 cut(s) | 184 |
| BspT104I | TTCGAA | 1 cut(s) | 1809 |
| BspT107I | GGYRCC | 1 cut(s) | 1126 |
| BspTI | CTTAAG | 1 cut(s) | 2585 |
| BspTNI | GGTCTC | 1 cut(s) | 62 |
| BsrDI | GCAATG | 2 cut(s) | 236, 1527 |
| BsrFI | RCCGGY | 1 cut(s) | 2143 |
| BsrI | ACTGG | 6 cut(s) | 477, 1619, 1633, 1875, 2276, 2487 |
| BssAI | RCCGGY | 1 cut(s) | 2143 |
| BssECI | CCNNGG | 6 cut(s) | 402, 856, 1421, 1835, 1957, 2288 |
| BssNI | GRCGYC | 5 cut(s) | 429, 1127, 2250, 2482, 2559 |
| BssT1I | CCWWGG | 3 cut(s) | 402, 1421, 1835 |
| Bst4CI | ACNGT | 9 cut(s) | 883, 1043, 1066, 1674, 1709, 2666, 2699, 2714, 2806 |
| Bst6I | CTCTTC | 4 cut(s) | 184, 1259, 1302, 1325 |
| BstACI | GRCGYC | 5 cut(s) | 429, 1127, 2250, 2482, 2559 |
| BstAFI | CTTAAG | 1 cut(s) | 2585 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 1529 |
| BstBAI | YACGTR | 2 cut(s) | 486, 1943 |
| BstBI | TTCGAA | 1 cut(s) | 1809 |
| BstC8I | GCNNGC | 4 cut(s) | 474, 1525, 1792, 1968 |
| BstDSI | CCRYGG | 1 cut(s) | 1421 |
| BstEII | GGTNACC | 2 cut(s) | 1133, 1597 |
| BstF5I | GGATG | 9 cut(s) | 177, 412, 613, 1281, 1318, 1846, 1892, 2425, 2690 |
| BstFNI | CGCG | 1 cut(s) | 1305 |
| BstH2I | RGCGCY | 2 cut(s) | 810, 1130 |
| BstHHI | GCGC | 2 cut(s) | 809, 1129 |
| BstMAI | GTCTC | 7 cut(s) | 53, 62, 188, 1286, 2357, 2565, 2691 |
| BstMCI | CGRYCG | 1 cut(s) | 832 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 119, 943, 1474, 1529, 1538 |
| BstNSI | RCATGY | 3 cut(s) | 788, 1527, 2003 |
| BstPI | GGTNACC | 2 cut(s) | 1133, 1597 |
| BstSFI | CTRYAG | 4 cut(s) | 331, 673, 935, 1440 |
| BstSLI | GKGCMC | 2 cut(s) | 684, 995 |
| BstUI | CGCG | 1 cut(s) | 1305 |
| BstV1I | GCAGC | 2 cut(s) | 637, 1644 |
| BstV2I | GAAGAC | 2 cut(s) | 981, 2347 |
| BstX2I | RGATCY | 5 cut(s) | 613, 711, 1817, 2524, 2630 |
| BstXI | CCANNNNNNTGG | 3 cut(s) | 744, 1032, 2208 |
| BstYI | RGATCY | 5 cut(s) | 613, 711, 1817, 2524, 2630 |
| Bsu15I | ATCGAT | 1 cut(s) | 1371 |
| BsuI | GTATCC | 3 cut(s) | 861, 1055, 2394 |
| BsuRI | GGCC | 5 cut(s) | 456, 472, 1446, 1746, 2008 |
| BsuTUI | ATCGAT | 1 cut(s) | 1371 |
| BtgI | CCRYGG | 1 cut(s) | 1421 |
| BtgZI | GCGATG | 2 cut(s) | 568, 1570 |
| BtrI | CACGTC | 2 cut(s) | 2153, 2353 |
| BtsCI | GGATG | 9 cut(s) | 177, 412, 613, 1281, 1318, 1846, 1892, 2425, 2690 |
| BtsI | GCAGTG | 2 cut(s) | 139, 944 |
| BtsIMutI | CAGTG | 5 cut(s) | 58, 139, 944, 1039, 1071 |
| Cac8I | GCNNGC | 4 cut(s) | 474, 1525, 1792, 1968 |
| CaiI | CAGNNNCTG | 3 cut(s) | 810, 1070, 2089 |
| CciI | TCATGA | 1 cut(s) | 2182 |
| CfoI | GCGC | 2 cut(s) | 809, 1129 |
| Cfr10I | RCCGGY | 1 cut(s) | 2143 |
| Cfr13I | GGNCC | 2 cut(s) | 200, 758 |
| ClaI | ATCGAT | 1 cut(s) | 1371 |
| CseI | GACGC | 5 cut(s) | 278, 437, 2087, 2471, 2582 |
| Csp6I | GTAC | 6 cut(s) | 313, 1575, 1705, 1716, 2667, 2700 |
| CspAI | ACCGGT | 1 cut(s) | 2143 |
| CviQI | GTAC | 6 cut(s) | 313, 1575, 1705, 1716, 2667, 2700 |
| DinI | GGCGCC | 1 cut(s) | 1128 |
| DraI | TTTAAA | 1 cut(s) | 1536 |
| EaeI | YGGCCR | 3 cut(s) | 470, 1744, 2006 |
| Eam1104I | CTCTTC | 4 cut(s) | 184, 1259, 1302, 1325 |
| EarI | CTCTTC | 4 cut(s) | 184, 1259, 1302, 1325 |
| Eco130I | CCWWGG | 3 cut(s) | 402, 1421, 1835 |
| Eco24I | GRGCYC | 2 cut(s) | 551, 2296 |
| Eco31I | GGTCTC | 1 cut(s) | 62 |
| Eco32I | GATATC | 1 cut(s) | 1369 |
| Eco47I | GGWCC | 2 cut(s) | 200, 758 |
| Eco57I | CTGAAG | 5 cut(s) | 1212, 1508, 1800, 2488, 2619 |
| Eco88I | CYCGRG | 2 cut(s) | 102, 2289 |
| Eco91I | GGTNACC | 2 cut(s) | 1133, 1597 |
| EcoO109I | RGGNCCY | 1 cut(s) | 200 |
| EcoO65I | GGTNACC | 2 cut(s) | 1133, 1597 |
| EcoRI | GAATTC | 1 cut(s) | 1910 |
| EcoRV | GATATC | 1 cut(s) | 1369 |
| EcoT14I | CCWWGG | 3 cut(s) | 402, 1421, 1835 |
| EcoT22I | ATGCAT | 2 cut(s) | 1529, 1894 |
| EcoT38I | GRGCYC | 2 cut(s) | 551, 2296 |
| EgeI | GGCGCC | 1 cut(s) | 1128 |
| EheI | GGCGCC | 1 cut(s) | 1128 |
| ErhI | CCWWGG | 3 cut(s) | 402, 1421, 1835 |
| Esp3I | CGTCTC | 1 cut(s) | 2565 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 189, 221, 2617, 2649 |
| FaqI | GGGAC | 4 cut(s) | 509, 1817, 2469, 2774 |
| FauI | CCCGC | 2 cut(s) | 597, 1787 |
| FauNDI | CATATG | 1 cut(s) | 1363 |
| FblI | GTMKAC | 1 cut(s) | 2815 |
| Fnu4HI | GCNGC | 2 cut(s) | 651, 1633 |
| FokI | GGATG | 9 cut(s) | 184, 419, 620, 1288, 1325, 1853, 1879, 2432, 2677 |
| FriOI | GRGCYC | 2 cut(s) | 551, 2296 |
| Fsp4HI | GCNGC | 2 cut(s) | 651, 1633 |
| FspBI | CTAG | 9 cut(s) | 245, 872, 947, 1562, 1691, 1787, 1869, 2256, 2597 |
| GlaI | GCGC | 2 cut(s) | 808, 1128 |
| GluI | GCNGC | 2 cut(s) | 651, 1633 |
| GsaI | CCCAGC | 2 cut(s) | 797, 1164 |
| GsuI | CTGGAG | 2 cut(s) | 639, 1858 |
| HaeII | RGCGCY | 2 cut(s) | 810, 1130 |
| HaeIII | GGCC | 5 cut(s) | 456, 472, 1446, 1746, 2008 |
| HapII | CCGG | 4 cut(s) | 761, 1747, 2144, 2156 |
| HgaI | GACGC | 5 cut(s) | 278, 437, 2087, 2471, 2582 |
| HhaI | GCGC | 2 cut(s) | 809, 1129 |
| Hin1I | GRCGYC | 5 cut(s) | 429, 1127, 2250, 2482, 2559 |
| Hin6I | GCGC | 2 cut(s) | 807, 1127 |
| HinP1I | GCGC | 2 cut(s) | 807, 1127 |
| HincII | GTYRAC | 2 cut(s) | 790, 2468 |
| HindII | GTYRAC | 2 cut(s) | 790, 2468 |
| HindIII | AAGCTT | 3 cut(s) | 1475, 1613, 2591 |
| HpaII | CCGG | 4 cut(s) | 761, 1747, 2144, 2156 |
| HphI | GGTGA | 3 cut(s) | 107, 530, 917 |
| Hpy166II | GTNNAC | 8 cut(s) | 489, 790, 993, 1090, 1946, 1997, 2468, 2816 |
| Hpy8I | GTNNAC | 8 cut(s) | 489, 790, 993, 1090, 1946, 1997, 2468, 2816 |
| Hpy99I | CGWCG | 3 cut(s) | 10, 431, 2081 |
| HpyAV | CCTTC | 6 cut(s) | 242, 971, 1510, 1676, 2132, 2239 |
| HpyCH4III | ACNGT | 9 cut(s) | 883, 1043, 1066, 1674, 1709, 2666, 2699, 2714, 2806 |
| HpyCH4V | TGCA | 8 cut(s) | 241, 776, 937, 993, 1527, 1892, 2031, 2394 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 119, 943, 1474, 1529, 1538 |
| Hsp92I | GRCGYC | 5 cut(s) | 429, 1127, 2250, 2482, 2559 |
| HspAI | GCGC | 2 cut(s) | 807, 1127 |
| KasI | GGCGCC | 1 cut(s) | 1126 |
| Kpn2I | TCCGGA | 2 cut(s) | 760, 2155 |
| LguI | GCTCTTC | 1 cut(s) | 184 |
| LmnI | GCTCC | 5 cut(s) | 103, 546, 658, 1520, 1877 |
| Lsp1109I | GCAGC | 2 cut(s) | 637, 1644 |
| LweI | GCATC | 8 cut(s) | 25, 250, 785, 1547, 1879, 1901, 2018, 2215 |
| MaeI | CTAG | 9 cut(s) | 245, 872, 947, 1562, 1691, 1787, 1869, 2256, 2597 |
| MaeIII | GTNAC | 8 cut(s) | 253, 877, 1072, 1133, 1597, 2146, 2251, 2708 |
| MflI | RGATCY | 5 cut(s) | 613, 711, 1817, 2524, 2630 |
| MhlI | GDGCHC | 4 cut(s) | 551, 684, 995, 2296 |
| MlsI | TGGCCA | 2 cut(s) | 472, 2008 |
| MluCI | AATT | 7 cut(s) | 558, 659, 1081, 1186, 1414, 1910, 2602 |
| MluNI | TGGCCA | 2 cut(s) | 472, 2008 |
| Mly113I | GGCGCC | 1 cut(s) | 1127 |
| MlyI | GAGTC | 1 cut(s) | 2821 |
| MmeI | TCCRAC | 2 cut(s) | 812, 1603 |
| Mox20I | TGGCCA | 2 cut(s) | 472, 2008 |
| Mph1103I | ATGCAT | 2 cut(s) | 1529, 1894 |
| MroI | TCCGGA | 2 cut(s) | 760, 2155 |
| MroXI | GAANNNNTTC | 2 cut(s) | 1614, 2319 |
| MscI | TGGCCA | 2 cut(s) | 472, 2008 |
| MseI | TTAA | 7 cut(s) | 1050, 1535, 1901, 2513, 2586, 2618, 2820 |
| MslI | CAYNNNNRTG | 4 cut(s) | 294, 742, 891, 2432 |
| Msp20I | TGGCCA | 2 cut(s) | 472, 2008 |
| MspA1I | CMGCKG | 1 cut(s) | 604 |
| MspCI | CTTAAG | 1 cut(s) | 2585 |
| MspI | CCGG | 4 cut(s) | 761, 1747, 2144, 2156 |
| Mva1269I | GAATGC | 1 cut(s) | 2179 |
| MvnI | CGCG | 1 cut(s) | 1305 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 119, 943, 1474, 1529, 1538 |
| NarI | GGCGCC | 1 cut(s) | 1127 |
| NciI | CCSGG | 1 cut(s) | 1748 |
| NcoI | CCATGG | 1 cut(s) | 1421 |
| NdeI | CATATG | 1 cut(s) | 1363 |
| NlaIV | GGNNCC | 3 cut(s) | 548, 1128, 2439 |
| NmuCI | GTSAC | 2 cut(s) | 2146, 2251 |
| NsiI | ATGCAT | 2 cut(s) | 1529, 1894 |
| NspI | RCATGY | 3 cut(s) | 788, 1527, 2003 |
| NspV | TTCGAA | 1 cut(s) | 1809 |
| PaeI | GCATGC | 1 cut(s) | 1527 |
| PagI | TCATGA | 1 cut(s) | 2182 |
| PciI | ACATGT | 1 cut(s) | 1999 |
| PciSI | GCTCTTC | 1 cut(s) | 184 |
| PctI | GAATGC | 1 cut(s) | 2179 |
| PdmI | GAANNNNTTC | 2 cut(s) | 1614, 2319 |
| PfeI | GAWTC | 9 cut(s) | 300, 327, 464, 1754, 1798, 1851, 1976, 2453, 2780 |
| PflMI | CCANNNNNTGG | 2 cut(s) | 810, 1479 |
| PinAI | ACCGGT | 1 cut(s) | 2143 |
| PkrI | GCNGC | 2 cut(s) | 652, 1634 |
| PleI | GAGTC | 1 cut(s) | 2820 |
| PluTI | GGCGCC | 1 cut(s) | 1130 |
| PpsI | GAGTC | 1 cut(s) | 2820 |
| Ppu21I | YACGTR | 2 cut(s) | 486, 1943 |
| PpuMI | RGGWCCY | 1 cut(s) | 200 |
| PscI | ACATGT | 1 cut(s) | 1999 |
| PshBI | ATTAAT | 1 cut(s) | 2618 |
| PsiI | TTATAA | 1 cut(s) | 2018 |
| Psp5II | RGGWCCY | 1 cut(s) | 200 |
| PspEI | GGTNACC | 2 cut(s) | 1133, 1597 |
| PspFI | CCCAGC | 2 cut(s) | 793, 1160 |
| PspN4I | GGNNCC | 3 cut(s) | 548, 1128, 2439 |
| PspPI | GGNCC | 2 cut(s) | 200, 758 |
| PspPPI | RGGWCCY | 1 cut(s) | 200 |
| PstI | CTGCAG | 1 cut(s) | 939 |
| PstNI | CAGNNNCTG | 3 cut(s) | 810, 1070, 2089 |
| PsuI | RGATCY | 5 cut(s) | 613, 711, 1817, 2524, 2630 |
| RsaI | GTAC | 6 cut(s) | 314, 1576, 1706, 1717, 2668, 2701 |
| RsaNI | GTAC | 6 cut(s) | 313, 1575, 1705, 1716, 2667, 2700 |
| RseI | CAYNNNNRTG | 4 cut(s) | 294, 742, 891, 2432 |
| SapI | GCTCTTC | 1 cut(s) | 184 |
| SaqAI | TTAA | 7 cut(s) | 1050, 1535, 1901, 2513, 2586, 2618, 2820 |
| SatI | GCNGC | 2 cut(s) | 651, 1633 |
| Sau96I | GGNCC | 2 cut(s) | 200, 758 |
| SchI | GAGTC | 1 cut(s) | 2821 |
| SduI | GDGCHC | 4 cut(s) | 551, 684, 995, 2296 |
| SfaNI | GCATC | 8 cut(s) | 25, 250, 785, 1547, 1879, 1901, 2018, 2215 |
| SfcI | CTRYAG | 4 cut(s) | 331, 673, 935, 1440 |
| SfoI | GGCGCC | 1 cut(s) | 1128 |
| SfuI | TTCGAA | 1 cut(s) | 1809 |
| SinI | GGWCC | 2 cut(s) | 200, 758 |
| SmiMI | CAYNNNNRTG | 4 cut(s) | 294, 742, 891, 2432 |
| SmlI | CTYRAG | 3 cut(s) | 258, 1724, 2585 |
| SmoI | CTYRAG | 3 cut(s) | 258, 1724, 2585 |
| SpeI | ACTAGT | 1 cut(s) | 2255 |
| SphI | GCATGC | 1 cut(s) | 1527 |
| Sse9I | AATT | 7 cut(s) | 558, 659, 1081, 1186, 1414, 1910, 2602 |
| SsiI | CCGC | 5 cut(s) | 604, 828, 968, 1303, 1794 |
| SspDI | GGCGCC | 1 cut(s) | 1126 |
| SspI | AATATT | 1 cut(s) | 2748 |
| SspMI | CTAG | 9 cut(s) | 245, 872, 947, 1562, 1691, 1787, 1869, 2256, 2597 |
| StyI | CCWWGG | 3 cut(s) | 402, 1421, 1835 |
| TaaI | ACNGT | 9 cut(s) | 883, 1043, 1066, 1674, 1709, 2666, 2699, 2714, 2806 |
| TaqI | TCGA | 8 cut(s) | 87, 495, 630, 1328, 1371, 1809, 1881, 2223 |
| TasI | AATT | 7 cut(s) | 558, 659, 1081, 1186, 1414, 1910, 2602 |
| TatI | WGTACW | 2 cut(s) | 312, 1574 |
| TfiI | GAWTC | 9 cut(s) | 300, 327, 464, 1754, 1798, 1851, 1976, 2453, 2780 |
| Tru1I | TTAA | 7 cut(s) | 1050, 1535, 1901, 2513, 2586, 2618, 2820 |
| Tru9I | TTAA | 7 cut(s) | 1050, 1535, 1901, 2513, 2586, 2618, 2820 |
| TscAI | CASTG | 5 cut(s) | 58, 139, 944, 1046, 1071 |
| TseFI | GTSAC | 2 cut(s) | 2146, 2251 |
| TseI | GCWGC | 2 cut(s) | 650, 1632 |
| Tsp45I | GTSAC | 2 cut(s) | 2146, 2251 |
| TspGWI | ACGGA | 2 cut(s) | 1288, 2658 |
| TspRI | CASTG | 5 cut(s) | 58, 139, 944, 1046, 1071 |
| Van91I | CCANNNNNTGG | 2 cut(s) | 810, 1479 |
| Vha464I | CTTAAG | 1 cut(s) | 2585 |
| VneI | GTGCAC | 1 cut(s) | 991 |
| VpaK11BI | GGWCC | 2 cut(s) | 200, 758 |
| VspI | ATTAAT | 1 cut(s) | 2618 |
| XapI | RAATTY | 4 cut(s) | 558, 1081, 1186, 1910 |
| XbaI | TCTAGA | 2 cut(s) | 244, 2596 |
| XceI | RCATGY | 3 cut(s) | 788, 1527, 2003 |
| XcmI | CCANNNNNNNNNTGG | 2 cut(s) | 1032, 1923 |
| XmiI | GTMKAC | 1 cut(s) | 2815 |
| XmnI | GAANNNNTTC | 2 cut(s) | 1614, 2319 |
| XspI | CTAG | 9 cut(s) | 245, 872, 947, 1562, 1691, 1787, 1869, 2256, 2597 |
| ZraI | GACGTC | 2 cut(s) | 2251, 2560 |
| Zsp2I | ATGCAT | 2 cut(s) | 1529, 1894 |