Rmu_sc0001461.1_g000056

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001461.1
OG0000630
Physical Location & Seq
Reverse (-)
199150 .. 205984
6835 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0001461.1_g000056.1.cds

Sequence Viewer

Length: 1650 bp
atgccgggggagacgattttcgttcctagtggatggtggcactgtgtcctaaatcttgaaccatctattgctgttacccagaactttgtaaattctaagaactttgaatttgtgtgcttggatatggcacctggttatcgtcataagggagtttgccgtgctggattacttgcagatgatgaaggcagtattgaggattccacaaatcatatactctatgataaagatgattataattcctcagatatgactaggaaagtaaaaagaggagcatgtcttgcactaaatgcaagcaaatggtcagaatccctggcagaaatttgtgccttccacaaattgccttctcgaacagatgatgagaggcttcctgttggcacgggtagcaatcctcttgagttctgcagtctcctaggcaaagtcaaatcccctctgaaaaatcacatacctgacgttttggcaagtggggtcatttatcttgaaaatggaacttataaaattgtaccttgtgatggcaacggagttcctgatgtgattgctaagtgcaattttattccggagaaggtcaaaggagatgtttctccttttggtgtatggaggaagaaacaatttgaatacagaaaagctggggtctcaacaaacgaatccatcagttcagctgaatatacaaggatatggccatatctcataacaaaacgatgcaaaggaaaaatatatgcagagttaagagatactgtgtcacgggaagatgaattgaacttagcttccttcctgggagagcagctccgtaacctccatctcttgccccatccacctcttaatatctcaactttctcagatattgaacaggaatcagattttcccttcaccagcggtagtatagaagctgttcccaataaatcaaacattcctgctgaatgggatatattcattagaactctacccaagaagaagaaggatgtctcaagtcgcttgattaaatggggagatccaattcctagcacactcatagagaaagttgacaaatacatcccagatgattttgccaagtttctgtacatattgaaggatgaaaatggtgtaagcaatgttggtaaaagttgttcgtggatacactctgatatcatggatgacaacattcacatggatctatgtggttttaattcttgcgtcattggcaatgccaaaaacacttgcctggtaaagaatggttccttgaatgttgatggtgaccgtgcagagaggagaacatggtgccctagccacattcttgatttcagtaatctttctataggtgatcccatctatgatttgatacccttatacttggatatattcagaggagattgctgcctccttaagagatttttagatagttataaacttcctttagtaagacaagcatcacctcatgagtatattgagggtggcgacaagtttggacgactttcataccatgccatgtgctactgtattctacatgaagagaatgtcctgggagcaatttttagtctctgggacgaactcaagatggcaaaatcatgggaagaagttgaacaggtcgtatggggcgagctaaacaactacaaaggctttcctggatcatccacatctctctctttgttggagccggaaactccctcaacattgtaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
APH Cupin_8 F-box F-box-like JmjC

Protein Analysis

549

Amino Acids

61.72

Weight (kDa)

5.5

Isoelectric Point (pI)

41.93

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000630)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G78280
fragaria_vesca FvH4_5g36800 FvH4_5g36800 FvH4_5g36800 FvH4_5g36800 FvH4_5g36800 FvH4_5g36800 FvH4_5g36800 FvH4_5g36800 FvH4_5g36800 FvH4_5g36800 FvH4_5g36800 FvH4_5g36800 FvH4_5g36800 FvH4_5g36800 FvH4_5g36800 FvH4_5g36800 FvH4_5g36800 FvH4_5g36800
malus_domestica MD08G1186800.v1.1 MD15G1372700.v1.1
prunus_persica Prupe.1G521300_v2.0.a1 Prupe.1G521300_v2.0.a1
pyrus_communis pycom08g16060 pycom15g33370
rosa_chinensis RchiOBHm_Chr2g0139391 RchiOBHm_Chr7g0242931 RchiOBHm_Chr7g0242951 RchiOBHm_Chr7g0243001 RchiOBHm_Chr7g0243011 RchiOBHm_Chr7g0243141 RchiOBHm_Chr7g0243151 RchiOBHm_Chr7g0243351
rosa_laevigata RLG00000000853 RLG00000000857 RLG00000000858 RLG00000000863 RLG00000000867
rosa_multiflora Rmu_co8457323.1_g000001 Rmu_sc0000083.1_g000017 Rmu_sc0000679.1_g000021 Rmu_sc0001036.1_g000029 Rmu_sc0001036.1_g000032 Rmu_sc0001176.1_g000015 Rmu_sc0001461.1_g000002 Rmu_sc0001461.1_g000003 Rmu_sc0001461.1_g000056 Rmu_sc0001461.1_g000057 Rmu_sc0001461.1_g000098 Rmu_sc0001461.1_g000105 Rmu_sc0004165.1_g000084 Rmu_sc0006141.1_g000004 Rmu_sc0033806.1_g000001
rosa_roxburghii Rroxscaffold_3G00222160 Rroxscaffold_3G00222220 Rroxscaffold_4G00316290
rosa_rugosa Rorug07G0318400 Rorug07G0318400 Rorug07G0318600
rosa_samantha Rh4CG066500 Rh5DG444300 Rh7AG473700 Rh7AG473900 Rh7AG474000 Rh7AG474600 Rh7AG474800 Rh7BG444500 Rh7BG444700 Rh7CG490700 Rh7CG490800 Rh7CG491000 Rh7DG458900 Rh7DG459000 Rh7DG459300
rosa_wichuraiana Rw7G038970 Rw7G039050 Rw7G039420 Rw7G039480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 234, 492, 1377
AccB1I GGYRCC 2 cut(s) 127, 1251
AccIII TCCGGA 1 cut(s) 553
AciI CCGC 1 cut(s) 870
AclWI GGATC 4 cut(s) 980, 1152, 1289, 1606
AcoI YGGCCR 1 cut(s) 674
AcsI RAATTY 3 cut(s) 91, 107, 318
AfaI GTAC 2 cut(s) 501, 1055
AfiI CCNNNNNNNGG 1 cut(s) 509
AflII CTTAAG 1 cut(s) 1355
AgsI TTSAA 9 cut(s) 59, 107, 479, 611, 754, 842, 1063, 1216, 1553
AjnI CCWGG 6 cut(s) 130, 309, 768, 1194, 1491, 1594
AloI GAACNNNNNNTCC 2 cut(s) 746, 778
AluBI AGCT 6 cut(s) 623, 656, 761, 781, 884, 1573
AluI AGCT 6 cut(s) 623, 656, 761, 781, 884, 1573
Alw26I GTCTC 5 cut(s) 5, 410, 634, 964, 1514
AlwI GGATC 4 cut(s) 980, 1152, 1289, 1606
Aor13HI TCCGGA 1 cut(s) 553
AoxI GGCC 1 cut(s) 674
ApeKI GCWGC 2 cut(s) 778, 1347
ApoI RAATTY 3 cut(s) 91, 107, 318
Asp700I GAANNNNTTC 1 cut(s) 885
AspA2I CCTAGG 1 cut(s) 409
AsuC2I CCSGG 1 cut(s) 6
AsuHPI GGTGA 4 cut(s) 856, 1238, 1304, 1395
AvrII CCTAGG 1 cut(s) 409
BaeGI GKGCMC 1 cut(s) 1256
BalI TGGCCA 1 cut(s) 676
BanI GGYRCC 2 cut(s) 127, 1251
BbvI GCAGC 2 cut(s) 790, 1334
BccI CCATC 9 cut(s) 27, 70, 503, 653, 801, 813, 1217, 1307, 1522
BceAI ACGGC 1 cut(s) 141
BciT130I CCWGG 6 cut(s) 132, 311, 770, 1196, 1493, 1596
BciVI GTATCC 1 cut(s) 1101
BcnI CCSGG 1 cut(s) 6
BcoDI GTCTC 5 cut(s) 5, 410, 634, 964, 1514
BfaI CTAG 5 cut(s) 27, 252, 410, 996, 1257
BfmI CTRYAG 2 cut(s) 400, 1287
BfrI CTTAAG 1 cut(s) 1355
BfuI GTATCC 1 cut(s) 1101
BisI GCNGC 2 cut(s) 779, 1348
BlnI CCTAGG 1 cut(s) 409
BlsI GCNGC 2 cut(s) 780, 1349
Bme1390I CCNGG 7 cut(s) 6, 132, 311, 770, 1196, 1493, 1596
BmiI GGNNCC 4 cut(s) 129, 1210, 1253, 1626
BmrFI CCNGG 7 cut(s) 6, 132, 311, 770, 1196, 1493, 1596
BmsI GCATC 2 cut(s) 686, 1409
BplI GAGNNNNNCTC 2 cut(s) 765, 797
BpuEI CTTGAG 3 cut(s) 413, 946, 1508
BpuMI CCSGG 1 cut(s) 6
BsaI GGTCTC 1 cut(s) 634
BsaJI CCNNGG 5 cut(s) 5, 309, 409, 769, 1492
BsaWI WCCGGW 1 cut(s) 553
Bsc4I CCNNNNNNNGG 1 cut(s) 509
Bse3DI GCAATG 2 cut(s) 1090, 1183
BseAI TCCGGA 1 cut(s) 553
BseBI CCWGG 6 cut(s) 132, 311, 770, 1196, 1493, 1596
BseDI CCNNGG 5 cut(s) 5, 309, 409, 769, 1492
BseGI GGATG 7 cut(s) 38, 805, 961, 1026, 1072, 1132, 1601
BseLI CCNNNNNNNGG 1 cut(s) 509
BseMI GCAATG 2 cut(s) 1090, 1183
BseMII CTCAG 2 cut(s) 255, 846
BseRI GAGGAG 3 cut(s) 282, 1255, 1353
BseSI GKGCMC 1 cut(s) 1256
BseXI GCAGC 2 cut(s) 790, 1334
BseYI CCCAGC 1 cut(s) 623
BsgI GTGCAG 1 cut(s) 1254
BshFI GGCC 1 cut(s) 676
BshNI GGYRCC 2 cut(s) 127, 1251
BsiSI CCGG 3 cut(s) 5, 554, 1628
BslFI GGGAC 1 cut(s) 1529
BslI CCNNNNNNNGG 1 cut(s) 509
BsmAI GTCTC 5 cut(s) 5, 410, 634, 964, 1514
BsmBI CGTCTC 1 cut(s) 5
BsmFI GGGAC 1 cut(s) 1529
BsnI GGCC 1 cut(s) 676
Bso31I GGTCTC 1 cut(s) 634
Bsp1286I GDGCHC 1 cut(s) 1256
Bsp13I TCCGGA 1 cut(s) 553
Bsp1407I TGTACA 1 cut(s) 1053
Bsp143I GATC 4 cut(s) 985, 1144, 1294, 1598
BspACI CCGC 1 cut(s) 870
BspANI GGCC 1 cut(s) 676
BspCNI CTCAG 2 cut(s) 254, 845
BspEI TCCGGA 1 cut(s) 553
BspHI TCATGA 1 cut(s) 1408
BspLI GGNNCC 4 cut(s) 129, 1210, 1253, 1626
BspMAI CTGCAG 1 cut(s) 404
BspPI GGATC 4 cut(s) 980, 1152, 1289, 1606
BspT107I GGYRCC 2 cut(s) 127, 1251
BspTI CTTAAG 1 cut(s) 1355
BspTNI GGTCTC 1 cut(s) 634
BsrDI GCAATG 2 cut(s) 1090, 1183
BsrGI TGTACA 1 cut(s) 1053
BssECI CCNNGG 5 cut(s) 5, 309, 409, 769, 1492
BssMI GATC 4 cut(s) 985, 1144, 1294, 1598
BssT1I CCWWGG 1 cut(s) 409
Bst2UI CCWGG 6 cut(s) 132, 311, 770, 1196, 1493, 1596
Bst4CI ACNGT 4 cut(s) 44, 733, 1232, 1469
Bst6I CTCTTC 1 cut(s) 1476
BstAFI CTTAAG 1 cut(s) 1355
BstAPI GCANNNNNTGC 2 cut(s) 278, 287
BstAUI TGTACA 1 cut(s) 1053
BstC8I GCNNGC 2 cut(s) 292, 1571
BstDEI CTNAG 5 cut(s) 96, 241, 537, 757, 832
BstEII GGTNACC 1 cut(s) 1226
BstF5I GGATG 7 cut(s) 38, 805, 961, 1026, 1072, 1132, 1601
BstKTI GATC 4 cut(s) 988, 1147, 1297, 1601
BstMAI GTCTC 5 cut(s) 5, 410, 634, 964, 1514
BstMBI GATC 4 cut(s) 985, 1144, 1294, 1598
BstMWI GCNNNNNNNGC 4 cut(s) 278, 287, 381, 1173
BstNI CCWGG 6 cut(s) 132, 311, 770, 1196, 1493, 1596
BstNSI RCATGY 1 cut(s) 276
BstPI GGTNACC 1 cut(s) 1226
BstSCI CCNGG 7 cut(s) 4, 130, 309, 768, 1194, 1491, 1594
BstSFI CTRYAG 2 cut(s) 400, 1287
BstSLI GKGCMC 1 cut(s) 1256
BstV1I GCAGC 2 cut(s) 790, 1334
BstX2I RGATCY 2 cut(s) 985, 1144
BstYI RGATCY 2 cut(s) 985, 1144
BsuI GTATCC 1 cut(s) 1101
BsuRI GGCC 1 cut(s) 676
BtsCI GGATG 7 cut(s) 38, 805, 961, 1026, 1072, 1132, 1601
BtsIMutI CAGTG 1 cut(s) 40
Cac8I GCNNGC 2 cut(s) 292, 1571
CciI TCATGA 1 cut(s) 1408
CseI GACGC 1 cut(s) 1156
CsiI ACCWGGT 1 cut(s) 130
Csp6I GTAC 2 cut(s) 500, 1054
CviAII CATG 9 cut(s) 273, 1123, 1141, 1248, 1409, 1454, 1459, 1478, 1539
CviQI GTAC 2 cut(s) 500, 1054
DdeI CTNAG 5 cut(s) 96, 241, 537, 757, 832
DpnI GATC 4 cut(s) 987, 1146, 1296, 1600
DpnII GATC 4 cut(s) 985, 1144, 1294, 1598
EaeI YGGCCR 1 cut(s) 674
Eam1104I CTCTTC 1 cut(s) 1476
EarI CTCTTC 1 cut(s) 1476
Eco130I CCWWGG 1 cut(s) 409
Eco31I GGTCTC 1 cut(s) 634
Eco32I GATATC 1 cut(s) 1120
Eco91I GGTNACC 1 cut(s) 1226
EcoO65I GGTNACC 1 cut(s) 1226
EcoRII CCWGG 6 cut(s) 130, 309, 768, 1194, 1491, 1594
EcoRV GATATC 1 cut(s) 1120
EcoT14I CCWWGG 1 cut(s) 409
ErhI CCWWGG 1 cut(s) 409
Esp3I CGTCTC 1 cut(s) 5
FaeI CATG 9 cut(s) 276, 1126, 1144, 1251, 1412, 1457, 1462, 1481, 1542
FaqI GGGAC 1 cut(s) 1529
FatI CATG 9 cut(s) 272, 1122, 1140, 1247, 1408, 1453, 1458, 1477, 1538
Fnu4HI GCNGC 2 cut(s) 779, 1348
FokI GGATG 7 cut(s) 45, 792, 968, 1013, 1079, 1139, 1588
Fsp4HI GCNGC 2 cut(s) 779, 1348
FspBI CTAG 5 cut(s) 27, 252, 410, 996, 1257
GluI GCNGC 2 cut(s) 779, 1348
GsaI CCCAGC 1 cut(s) 627
HaeIII GGCC 1 cut(s) 676
HapII CCGG 3 cut(s) 5, 554, 1628
HgaI GACGC 1 cut(s) 1156
Hin1II CATG 9 cut(s) 276, 1126, 1144, 1251, 1412, 1457, 1462, 1481, 1542
HincII GTYRAC 1 cut(s) 1018
HindII GTYRAC 1 cut(s) 1018
HinfI GANTC 4 cut(s) 197, 305, 641, 848
HpaII CCGG 3 cut(s) 5, 554, 1628
HphI GGTGA 4 cut(s) 856, 1238, 1304, 1395
Hpy166II GTNNAC 1 cut(s) 1018
Hpy188I TCNGA 7 cut(s) 244, 304, 432, 835, 853, 1117, 1337
Hpy188III TCNNGA 9 cut(s) 56, 345, 392, 476, 524, 554, 1268, 1409, 1525
Hpy8I GTNNAC 1 cut(s) 1018
HpyAV CCTTC 8 cut(s) 176, 337, 351, 553, 775, 871, 946, 1057
HpyCH4III ACNGT 4 cut(s) 44, 733, 1232, 1469
HpyCH4IV ACGT 1 cut(s) 450
HpyCH4V TGCA 8 cut(s) 173, 281, 290, 402, 543, 699, 716, 1235
HpyF10VI GCNNNNNNNGC 4 cut(s) 278, 287, 381, 1173
HpyF3I CTNAG 5 cut(s) 96, 241, 537, 757, 832
HpySE526I ACGT 1 cut(s) 450
Hsp92II CATG 9 cut(s) 276, 1126, 1144, 1251, 1412, 1457, 1462, 1481, 1542
Kpn2I TCCGGA 1 cut(s) 553
Kzo9I GATC 4 cut(s) 985, 1144, 1294, 1598
LmnI GCTCC 4 cut(s) 269, 786, 1496, 1624
Lsp1109I GCAGC 2 cut(s) 790, 1334
LweI GCATC 2 cut(s) 686, 1409
MabI ACCWGGT 1 cut(s) 130
MaeI CTAG 5 cut(s) 27, 252, 410, 996, 1257
MaeII ACGT 1 cut(s) 450
MaeIII GTNAC 4 cut(s) 73, 735, 785, 1226
MalI GATC 4 cut(s) 987, 1146, 1296, 1600
MboI GATC 4 cut(s) 985, 1144, 1294, 1598
MboII GAAGA 6 cut(s) 610, 755, 958, 961, 1493, 1556
MflI RGATCY 2 cut(s) 985, 1144
MhlI GDGCHC 1 cut(s) 1256
MlsI TGGCCA 1 cut(s) 676
MluNI TGGCCA 1 cut(s) 676
MmeI TCCRAC 1 cut(s) 1602
Mox20I TGGCCA 1 cut(s) 676
MroI TCCGGA 1 cut(s) 553
MroXI GAANNNNTTC 1 cut(s) 885
MscI TGGCCA 1 cut(s) 676
MseI TTAA 5 cut(s) 722, 816, 975, 1158, 1356
MslI CAYNNNNRTG 1 cut(s) 1139
Msp20I TGGCCA 1 cut(s) 676
MspA1I CMGCKG 2 cut(s) 656, 870
MspCI CTTAAG 1 cut(s) 1355
MspI CCGG 3 cut(s) 5, 554, 1628
MspR9I CCNGG 7 cut(s) 6, 132, 311, 770, 1196, 1493, 1596
MvaI CCWGG 6 cut(s) 132, 311, 770, 1196, 1493, 1596
MwoI GCNNNNNNNGC 4 cut(s) 278, 287, 381, 1173
NciI CCSGG 1 cut(s) 6
NdeII GATC 4 cut(s) 985, 1144, 1294, 1598
NlaIII CATG 9 cut(s) 276, 1126, 1144, 1251, 1412, 1457, 1462, 1481, 1542
NlaIV GGNNCC 4 cut(s) 129, 1210, 1253, 1626
NmuCI GTSAC 2 cut(s) 735, 1226
NspI RCATGY 1 cut(s) 276
PagI TCATGA 1 cut(s) 1408
PcsI WCGNNNNNNNCGW 1 cut(s) 1566
PdmI GAANNNNTTC 1 cut(s) 885
PfeI GAWTC 4 cut(s) 197, 305, 641, 848
PfoI TCCNGGA 1 cut(s) 1594
PkrI GCNGC 2 cut(s) 780, 1349
PsiI TTATAA 3 cut(s) 234, 492, 1377
Psp6I CCWGG 6 cut(s) 130, 309, 768, 1194, 1491, 1594
PspEI GGTNACC 1 cut(s) 1226
PspFI CCCAGC 1 cut(s) 623
PspGI CCWGG 6 cut(s) 130, 309, 768, 1194, 1491, 1594
PspN4I GGNNCC 4 cut(s) 129, 1210, 1253, 1626
PsrI GAACNNNNNNTAC 2 cut(s) 1084, 1116
PstI CTGCAG 1 cut(s) 404
PsuI RGATCY 2 cut(s) 985, 1144
PvuII CAGCTG 1 cut(s) 656
RsaI GTAC 2 cut(s) 501, 1055
RsaNI GTAC 2 cut(s) 500, 1054
RseI CAYNNNNRTG 1 cut(s) 1139
SaqAI TTAA 5 cut(s) 722, 816, 975, 1158, 1356
SatI GCNGC 2 cut(s) 779, 1348
Sau3AI GATC 4 cut(s) 985, 1144, 1294, 1598
ScrFI CCNGG 7 cut(s) 6, 132, 311, 770, 1196, 1493, 1596
SduI GDGCHC 1 cut(s) 1256
SexAI ACCWGGT 1 cut(s) 130
SfaNI GCATC 2 cut(s) 686, 1409
SfcI CTRYAG 2 cut(s) 400, 1287
SmiMI CAYNNNNRTG 1 cut(s) 1139
SmlI CTYRAG 4 cut(s) 392, 961, 1355, 1523
SmoI CTYRAG 4 cut(s) 392, 961, 1355, 1523
SsiI CCGC 1 cut(s) 870
SspMI CTAG 5 cut(s) 27, 252, 410, 996, 1257
StyD4I CCNGG 7 cut(s) 4, 130, 309, 768, 1194, 1491, 1594
StyI CCWWGG 1 cut(s) 409
TaaI ACNGT 4 cut(s) 44, 733, 1232, 1469
TaiI ACGT 1 cut(s) 453
TaqI TCGA 1 cut(s) 346
TatI WGTACW 1 cut(s) 1053
TfiI GAWTC 4 cut(s) 197, 305, 641, 848
Tru1I TTAA 5 cut(s) 722, 816, 975, 1158, 1356
Tru9I TTAA 5 cut(s) 722, 816, 975, 1158, 1356
TscAI CASTG 1 cut(s) 47
TseFI GTSAC 2 cut(s) 735, 1226
TseI GCWGC 2 cut(s) 778, 1347
Tsp45I GTSAC 2 cut(s) 735, 1226
TspDTI ATGAA 6 cut(s) 195, 762, 916, 1083, 1437, 1494
TspGWI ACGGA 2 cut(s) 531, 773
TspRI CASTG 1 cut(s) 47
Vha464I CTTAAG 1 cut(s) 1355
XapI RAATTY 3 cut(s) 91, 107, 318
XceI RCATGY 1 cut(s) 276
XmaJI CCTAGG 1 cut(s) 409
XmnI GAANNNNTTC 1 cut(s) 885
XspI CTAG 5 cut(s) 27, 252, 410, 996, 1257
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.