Rmu_sc0004165.1_g000084

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004165.1
OG0000630
Physical Location & Seq
Reverse (-)
328078 .. 330179
2102 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0004165.1_g000084.1.cds

Sequence Viewer

Length: 798 bp
atggaagtttccgaagcaactcccttccggccaagagaccgccggccggacgccctcggaagcttcctagtcctccacgacgagctcatctgtgccgtcttggagttgctctcccctcgcgacgttgctcgcctctcatgcgtcagcagggagaatgtgtcgtatgaacgtgatgaggcttgcagaaaaccactgagttttgatggttttctctggctgaaggcgtttgtcattgcctatctacgtggtgcaggattcgactctttgttcttgtacagaagattatacaggtgtcataccacattggatggtttttcttttgacaatgggaatgtggaaagaaaggacaagatcacattggaagagttttcttgtgattatgatgggaagaaaccggttttgctcactggattagctgatgcatggcctgcaaagcggacatgggcacttgaccactttttgcagaattatggagatacagctttcaagatttctcaaaggagctcacgtaaaatctcaatgaaattcaaggattatgtttcatatatgaaagttcagcatgatgaggatccgctttatatctttgatctcaagtttggagaggttgaacctggcttgttgaaagattacagtgtaccttatctatttcaagaggacttttttgatgtttcggaacaagacaagcgacctccatttcgatggctaattattggtccccagaggtctggtgcttcttggcatgttgatccagctctgaccagtgcctggaatacgcttctacgtggtcgtaaaagataa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
APH Cupin_8 F-box F-box-like JmjC

Protein Analysis

265

Amino Acids

30.85

Weight (kDa)

6.96

Isoelectric Point (pI)

45.75

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000630)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G78280
fragaria_vesca FvH4_5g36800 FvH4_5g36800 FvH4_5g36800 FvH4_5g36800 FvH4_5g36800 FvH4_5g36800 FvH4_5g36800 FvH4_5g36800 FvH4_5g36800 FvH4_5g36800 FvH4_5g36800 FvH4_5g36800 FvH4_5g36800 FvH4_5g36800 FvH4_5g36800 FvH4_5g36800 FvH4_5g36800 FvH4_5g36800
malus_domestica MD08G1186800.v1.1 MD15G1372700.v1.1
prunus_persica Prupe.1G521300_v2.0.a1 Prupe.1G521300_v2.0.a1
pyrus_communis pycom08g16060 pycom15g33370
rosa_chinensis RchiOBHm_Chr2g0139391 RchiOBHm_Chr7g0242931 RchiOBHm_Chr7g0242951 RchiOBHm_Chr7g0243001 RchiOBHm_Chr7g0243011 RchiOBHm_Chr7g0243141 RchiOBHm_Chr7g0243151 RchiOBHm_Chr7g0243351
rosa_laevigata RLG00000000853 RLG00000000857 RLG00000000858 RLG00000000863 RLG00000000867
rosa_multiflora Rmu_co8457323.1_g000001 Rmu_sc0000083.1_g000017 Rmu_sc0000679.1_g000021 Rmu_sc0001036.1_g000029 Rmu_sc0001036.1_g000032 Rmu_sc0001176.1_g000015 Rmu_sc0001461.1_g000002 Rmu_sc0001461.1_g000003 Rmu_sc0001461.1_g000056 Rmu_sc0001461.1_g000057 Rmu_sc0001461.1_g000098 Rmu_sc0001461.1_g000105 Rmu_sc0004165.1_g000084 Rmu_sc0006141.1_g000004 Rmu_sc0033806.1_g000001
rosa_roxburghii Rroxscaffold_3G00222160 Rroxscaffold_3G00222220 Rroxscaffold_4G00316290
rosa_rugosa Rorug07G0318400 Rorug07G0318400 Rorug07G0318600
rosa_samantha Rh4CG066500 Rh5DG444300 Rh7AG473700 Rh7AG473900 Rh7AG474000 Rh7AG474600 Rh7AG474800 Rh7BG444500 Rh7BG444700 Rh7CG490700 Rh7CG490800 Rh7CG491000 Rh7DG458900 Rh7DG459000 Rh7DG459300
rosa_wichuraiana Rw7G038970 Rw7G039050 Rw7G039420 Rw7G039480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 765
AccII CGCG 1 cut(s) 120
AciI CCGC 3 cut(s) 40, 436, 572
AclWI GGATC 3 cut(s) 563, 576, 740
AcoI YGGCCR 2 cut(s) 29, 44
AcsI RAATTY 1 cut(s) 524
AcuI CTGAAG 1 cut(s) 239
AcyI GRCGYC 1 cut(s) 51
AfaI GTAC 2 cut(s) 275, 636
AfiI CCNNNNNNNGG 2 cut(s) 46, 765
AgeI ACCGGT 1 cut(s) 394
AgsI TTSAA 5 cut(s) 487, 529, 608, 622, 650
AjnI CCWGG 2 cut(s) 610, 764
AluBI AGCT 6 cut(s) 63, 85, 416, 482, 504, 752
AluI AGCT 6 cut(s) 63, 85, 416, 482, 504, 752
Alw21I GWGCWC 2 cut(s) 87, 506
Alw26I GTCTC 1 cut(s) 30
AlwI GGATC 3 cut(s) 563, 576, 740
AlwNI CAGNNNCTG 1 cut(s) 765
AoxI GGCC 3 cut(s) 29, 44, 425
ApoI RAATTY 1 cut(s) 524
ArsI GACNNNNNNTTYG 2 cut(s) 678, 710
AsiGI ACCGGT 1 cut(s) 394
AspS9I GGNCC 1 cut(s) 713
AvaII GGWCC 1 cut(s) 713
BaeGI GKGCMC 1 cut(s) 448
BamHI GGATCC 1 cut(s) 568
BanII GRGCYC 2 cut(s) 87, 506
Bbv12I GWGCWC 2 cut(s) 87, 506
BccI CCATC 4 cut(s) 197, 302, 377, 693
BceAI ACGGC 1 cut(s) 80
BciT130I CCWGG 2 cut(s) 612, 766
BcoDI GTCTC 1 cut(s) 30
BfaI CTAG 1 cut(s) 68
Bme1390I CCNGG 2 cut(s) 612, 766
Bme18I GGWCC 1 cut(s) 713
BmgT120I GGNCC 1 cut(s) 713
BmiI GGNNCC 2 cut(s) 570, 715
BmrFI CCNGG 2 cut(s) 612, 766
BmsI GCATC 1 cut(s) 409
BplI GAGNNNNNCTC 2 cut(s) 95, 127
BpuEI CTTGAG 1 cut(s) 575
BsaAI YACGTR 3 cut(s) 245, 509, 782
BsaBI GATNNNNATC 1 cut(s) 567
BsaHI GRCGYC 1 cut(s) 51
BsaI GGTCTC 1 cut(s) 30
BsaJI CCNNGG 1 cut(s) 55
BsaWI WCCGGW 1 cut(s) 394
BsaXI ACNNNNNCTCC 4 cut(s) 143, 173, 493, 523
Bsc4I CCNNNNNNNGG 2 cut(s) 46, 765
Bse118I RCCGGY 2 cut(s) 42, 394
Bse1I ACTGG 2 cut(s) 412, 759
Bse3DI GCAATG 1 cut(s) 231
Bse8I GATNNNNATC 1 cut(s) 567
BseBI CCWGG 2 cut(s) 612, 766
BseDI CCNNGG 1 cut(s) 55
BseGI GGATG 1 cut(s) 313
BseJI GATNNNNATC 1 cut(s) 567
BseLI CCNNNNNNNGG 2 cut(s) 46, 765
BseMI GCAATG 1 cut(s) 231
BseMII CTCAG 1 cut(s) 185
BseNI ACTGG 2 cut(s) 412, 759
BseSI GKGCMC 1 cut(s) 448
BseX3I CGGCCG 1 cut(s) 44
BsgI GTGCAG 1 cut(s) 270
Bsh1236I CGCG 1 cut(s) 120
Bsh1285I CGRYCG 1 cut(s) 47
BshFI GGCC 3 cut(s) 31, 46, 427
BshTI ACCGGT 1 cut(s) 394
BsiEI CGRYCG 1 cut(s) 47
BsiHKAI GWGCWC 2 cut(s) 87, 506
BsiSI CCGG 4 cut(s) 28, 43, 47, 395
BslFI GGGAC 1 cut(s) 699
BslI CCNNNNNNNGG 2 cut(s) 46, 765
BsmAI GTCTC 1 cut(s) 30
BsmFI GGGAC 1 cut(s) 699
BsnI GGCC 3 cut(s) 31, 46, 427
Bso31I GGTCTC 1 cut(s) 30
Bsp1286I GDGCHC 3 cut(s) 87, 448, 506
Bsp1407I TGTACA 1 cut(s) 273
Bsp143I GATC 4 cut(s) 351, 568, 586, 745
Bsp68I TCGCGA 1 cut(s) 120
BspACI CCGC 3 cut(s) 40, 436, 572
BspANI GGCC 3 cut(s) 31, 46, 427
BspCNI CTCAG 1 cut(s) 186
BspFNI CGCG 1 cut(s) 120
BspLI GGNNCC 2 cut(s) 570, 715
BspPI GGATC 3 cut(s) 563, 576, 740
BspTNI GGTCTC 1 cut(s) 30
BsrDI GCAATG 1 cut(s) 231
BsrFI RCCGGY 2 cut(s) 42, 394
BsrGI TGTACA 1 cut(s) 273
BsrI ACTGG 2 cut(s) 412, 759
BssAI RCCGGY 2 cut(s) 42, 394
BssECI CCNNGG 1 cut(s) 55
BssMI GATC 4 cut(s) 351, 568, 586, 745
BssNI GRCGYC 1 cut(s) 51
Bst2UI CCWGG 2 cut(s) 612, 766
Bst4CI ACNGT 1 cut(s) 632
Bst6I CTCTTC 1 cut(s) 357
BstACI GRCGYC 1 cut(s) 51
BstAPI GCANNNNNTGC 1 cut(s) 428
BstAUI TGTACA 1 cut(s) 273
BstBAI YACGTR 3 cut(s) 245, 509, 782
BstC8I GCNNGC 4 cut(s) 44, 130, 181, 429
BstDEI CTNAG 1 cut(s) 194
BstF5I GGATG 1 cut(s) 313
BstFNI CGCG 1 cut(s) 120
BstKTI GATC 4 cut(s) 354, 571, 589, 748
BstMAI GTCTC 1 cut(s) 30
BstMBI GATC 4 cut(s) 351, 568, 586, 745
BstMCI CGRYCG 1 cut(s) 47
BstMWI GCNNNNNNNGC 3 cut(s) 138, 428, 433
BstNI CCWGG 2 cut(s) 612, 766
BstNSI RCATGY 1 cut(s) 743
BstSCI CCNGG 2 cut(s) 610, 764
BstSLI GKGCMC 1 cut(s) 448
BstUI CGCG 1 cut(s) 120
BstX2I RGATCY 1 cut(s) 568
BstXI CCANNNNNNTGG 2 cut(s) 699, 725
BstYI RGATCY 1 cut(s) 568
BstZI CGGCCG 1 cut(s) 44
BsuRI GGCC 3 cut(s) 31, 46, 427
BtsCI GGATG 1 cut(s) 313
BtsIMutI CAGTG 4 cut(s) 191, 405, 637, 766
BtuMI TCGCGA 1 cut(s) 120
Cac8I GCNNGC 4 cut(s) 44, 130, 181, 429
CaiI CAGNNNCTG 1 cut(s) 765
Cfr10I RCCGGY 2 cut(s) 42, 394
Cfr13I GGNCC 1 cut(s) 713
CseI GACGC 2 cut(s) 59, 130
Csp6I GTAC 2 cut(s) 274, 635
CspAI ACCGGT 1 cut(s) 394
CviAII CATG 5 cut(s) 138, 423, 441, 560, 740
CviQI GTAC 2 cut(s) 274, 635
DdeI CTNAG 1 cut(s) 194
DpnI GATC 4 cut(s) 353, 570, 588, 747
DpnII GATC 4 cut(s) 351, 568, 586, 745
EaeI YGGCCR 2 cut(s) 29, 44
EagI CGGCCG 1 cut(s) 44
Eam1104I CTCTTC 1 cut(s) 357
EarI CTCTTC 1 cut(s) 357
Ecl136II GAGCTC 2 cut(s) 85, 504
EclXI CGGCCG 1 cut(s) 44
Eco24I GRGCYC 2 cut(s) 87, 506
Eco31I GGTCTC 1 cut(s) 30
Eco47I GGWCC 1 cut(s) 713
Eco52I CGGCCG 1 cut(s) 44
Eco53kI GAGCTC 2 cut(s) 85, 504
Eco57I CTGAAG 1 cut(s) 239
EcoICRI GAGCTC 2 cut(s) 85, 504
EcoRII CCWGG 2 cut(s) 610, 764
EcoT22I ATGCAT 1 cut(s) 424
EcoT38I GRGCYC 2 cut(s) 87, 506
FaeI CATG 5 cut(s) 141, 426, 444, 563, 743
FaqI GGGAC 1 cut(s) 699
FatI CATG 5 cut(s) 137, 422, 440, 559, 739
FokI GGATG 1 cut(s) 320
FriOI GRGCYC 2 cut(s) 87, 506
FspBI CTAG 1 cut(s) 68
HaeIII GGCC 3 cut(s) 31, 46, 427
HapII CCGG 4 cut(s) 28, 43, 47, 395
HgaI GACGC 2 cut(s) 59, 130
Hin1I GRCGYC 1 cut(s) 51
Hin1II CATG 5 cut(s) 141, 426, 444, 563, 743
HindIII AAGCTT 1 cut(s) 61
HinfI GANTC 2 cut(s) 255, 260
HpaII CCGG 4 cut(s) 28, 43, 47, 395
Hpy166II GTNNAC 1 cut(s) 635
Hpy188I TCNGA 4 cut(s) 13, 59, 673, 756
Hpy188III TCNNGA 3 cut(s) 119, 487, 650
Hpy8I GTNNAC 1 cut(s) 635
Hpy99I CGWCG 2 cut(s) 83, 125
HpyAV CCTTC 2 cut(s) 34, 214
HpyCH4III ACNGT 1 cut(s) 632
HpyCH4IV ACGT 5 cut(s) 123, 169, 244, 508, 781
HpyCH4V TGCA 5 cut(s) 183, 251, 422, 431, 463
HpyF10VI GCNNNNNNNGC 3 cut(s) 138, 428, 433
HpyF3I CTNAG 1 cut(s) 194
HpySE526I ACGT 5 cut(s) 123, 169, 244, 508, 781
Hsp92I GRCGYC 1 cut(s) 51
Hsp92II CATG 5 cut(s) 141, 426, 444, 563, 743
KroI GCCGGC 1 cut(s) 42
KroNI GCCGGC 1 cut(s) 44
Kzo9I GATC 4 cut(s) 351, 568, 586, 745
LmnI GCTCC 1 cut(s) 501
LweI GCATC 1 cut(s) 409
MaeI CTAG 1 cut(s) 68
MaeII ACGT 5 cut(s) 123, 169, 244, 508, 781
MalI GATC 4 cut(s) 353, 570, 588, 747
MboI GATC 4 cut(s) 351, 568, 586, 745
MboII GAAGA 3 cut(s) 291, 374, 400
MflI RGATCY 1 cut(s) 568
MhlI GDGCHC 3 cut(s) 87, 448, 506
MluCI AATT 3 cut(s) 466, 524, 705
MlyI GAGTC 1 cut(s) 254
Mph1103I ATGCAT 1 cut(s) 424
MroNI GCCGGC 1 cut(s) 42
MslI CAYNNNNRTG 1 cut(s) 697
MspI CCGG 4 cut(s) 28, 43, 47, 395
MspR9I CCNGG 2 cut(s) 612, 766
MvaI CCWGG 2 cut(s) 612, 766
MvnI CGCG 1 cut(s) 120
MwoI GCNNNNNNNGC 3 cut(s) 138, 428, 433
NaeI GCCGGC 1 cut(s) 44
NdeII GATC 4 cut(s) 351, 568, 586, 745
NgoMIV GCCGGC 1 cut(s) 42
NlaIII CATG 5 cut(s) 141, 426, 444, 563, 743
NlaIV GGNNCC 2 cut(s) 570, 715
NruI TCGCGA 1 cut(s) 120
NsiI ATGCAT 1 cut(s) 424
NspI RCATGY 1 cut(s) 743
PdiI GCCGGC 1 cut(s) 44
PfeI GAWTC 1 cut(s) 255
PflMI CCANNNNNTGG 1 cut(s) 765
PinAI ACCGGT 1 cut(s) 394
PleI GAGTC 1 cut(s) 254
PpsI GAGTC 1 cut(s) 254
Ppu21I YACGTR 3 cut(s) 245, 509, 782
Psp124BI GAGCTC 2 cut(s) 87, 506
Psp6I CCWGG 2 cut(s) 610, 764
PspGI CCWGG 2 cut(s) 610, 764
PspN4I GGNNCC 2 cut(s) 570, 715
PspPI GGNCC 1 cut(s) 713
PstNI CAGNNNCTG 1 cut(s) 765
PsuI RGATCY 1 cut(s) 568
RruI TCGCGA 1 cut(s) 120
RsaI GTAC 2 cut(s) 275, 636
RsaNI GTAC 2 cut(s) 274, 635
RseI CAYNNNNRTG 1 cut(s) 697
SacI GAGCTC 2 cut(s) 87, 506
Sau3AI GATC 4 cut(s) 351, 568, 586, 745
Sau96I GGNCC 1 cut(s) 713
SchI GAGTC 1 cut(s) 254
ScrFI CCNGG 2 cut(s) 612, 766
SduI GDGCHC 3 cut(s) 87, 448, 506
SfaNI GCATC 1 cut(s) 409
SinI GGWCC 1 cut(s) 713
SmiMI CAYNNNNRTG 1 cut(s) 697
SmlI CTYRAG 1 cut(s) 590
SmoI CTYRAG 1 cut(s) 590
Sse9I AATT 3 cut(s) 466, 524, 705
SsiI CCGC 3 cut(s) 40, 436, 572
SspMI CTAG 1 cut(s) 68
SstI GAGCTC 2 cut(s) 87, 506
StyD4I CCNGG 2 cut(s) 610, 764
TaaI ACNGT 1 cut(s) 632
TaiI ACGT 5 cut(s) 126, 172, 247, 511, 784
TaqI TCGA 2 cut(s) 258, 697
TasI AATT 3 cut(s) 466, 524, 705
TatI WGTACW 1 cut(s) 273
TfiI GAWTC 1 cut(s) 255
TscAI CASTG 4 cut(s) 198, 412, 637, 766
TspDTI ATGAA 4 cut(s) 180, 531, 536, 563
TspRI CASTG 4 cut(s) 198, 412, 637, 766
Van91I CCANNNNNTGG 1 cut(s) 765
VpaK11BI GGWCC 1 cut(s) 713
XapI RAATTY 1 cut(s) 524
XceI RCATGY 1 cut(s) 743
XspI CTAG 1 cut(s) 68
Zsp2I ATGCAT 1 cut(s) 424
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.