MD08G1186800.v1.1

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0000630
Physical Location & Seq
Forward (+)
23531368 .. 23540505
9138 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1186800.v1.1.491

Sequence Viewer

Length: 2946 bp
ATGGAGAATTCTCAGGCCCACGCGTTAGGGCTCACAGATCGCCGGCCGGCCGCTCTCGGAGACTTCCGAGTCCTTCCTGACGACCTCATCTGCGACATCCTGGAATACCTCCCTCCTCGCGACGTCGCTCGCCTCGCTTGCGTTAGCAGTGTGATGTACATACTATGCAATGAAGAGCCACTTTGGATGAGTTTATGCCTCAGCAAAGTAAATGGTCCGCTCCAGTATAAAGGCTCGTGGAAGAAGACGGCACTTGATTTGGAGCATGTACCATATGAATGCGACGAGGCTGGCAGAAAACCACTTTATTTTGATGGATTCAACTCATTGTTCTTGTACAGAAGATTATACAGGTGCTATACCACATTGGATGCTTTTTCTTTTGACGATGGGAATGTGGAAAGGAAGAAAGACCTCAATTTGGAAGACTTTTCTTGTGACTATGATGGAAAGAAACCGGTTTTGCTTACTGGATTAGCTGATGCTTGGCCTGCAAGGCATACATGGACATTTGACCAGTTATTGCAGAAATATGGAGATACAGCTTTCAAAATTTCTCAAAGGAGTGCTCGCAAAGTCTCAATGAAATTCAAGGATTATGTTTCATACTTGAAAATTCAACATGATGAGGATCCCCTATATATTTTTGACCACAAGTTTGGAGAGGTTGCACCTGCCTTGTTGAAAGATTACTGTGTGCCTTGTCTATTTCAAGAGGACTTTTTTGATGTTTTAGATGGAGACAAGCGACCACCATTTAGGTGGCTCATTATTGGGCCACAGAGGTCTGGTGCCTCCTGGCATGTTGATCCAGCCCTGACCAGTGCCTGGAATACGCTACTAGTTGGCCGTAAGAGGTGGGCCCTGTATCCACCAGGAAGAGTACCACTAGGTGTCACGGTACATGTAAACGAAGAAGATGGTGATGTCAATATCGAAACTCCATCTTCACTGCAGTGGTGGCTAGATTTTTATCCACTTCTTGCTGATAAAGACAAACCGATAGAGTGTACCCAACTTCCAGGGGAAACGATTTTCGTTCCAAGTGGATGGTGGCACTGTGTCTTAAATCTGGAACCAAGTATTGCCGTTACCCAGAACTTTGTAAATCCCAAAAATTTTGAATTTGTGTGTTTAGACATGGCACCTGGTTATCATCATAAGGGAGTTTGCCGTGCTGGATTACTTGCACATGCAGAAGGAAATTCAGATGATGCCACAAATAATAAAGATGATTCTGGTATCTCTGATCTGATTAGGAAAGTGAAAAGAGTCAGGACTCTCGAACCTGGAGAAAAAAAGAATGCTGATGTTGCTTCTAATGACCATGATTTACAACAAAGAATTTCCCAGGGGTTTTCTTATGACATAAATTTCTTAGCCATGTATCTAGATAAAGAGAGAGATCACTACAACTCTCCTTGGAGCTCTGGCAACTGCATAGGACAACGAGAAATGAGAGAATGGTTATTTAAGCTTTGGTTTGCAAAAACAGGAATGAGAGACTTAATCTGGAAGGGAGCTTGTCTTGCATTAAATGCTGGGAGATGGTCTGAATGCCTGTCAGAAATTTGTGCATTCCATAATTTGCCTTATCCAGCAGAAGATGAGAAGCTTCCTGTTGGCATGGGTAGCAATCCTGTTTATCTCTTGAGCGACTGCGTAGTCAAAATTTTTGTTGAAGAAGGGCTGGAAAGATCACTTTATGGTCTAGGCACAGAGCTAGAGTTTTACAATCTCCTATGCAAAGTCAACTCCCCTCTGAAGAATCACATGCCTGATGTTTTGGAAAGTGGGATCATTTATCTTGAGAATGGAATTTACAGAATTGTACCATGGGATGCCAAAACAGTTCCTGATGTGATTGCCAGATGCAATCTTATTCCTGAGAAGTTAAATGCAGATGTTTGTCCTTTTGGCGTATGGAGCAAGAAACAGTTTAATTACAGAAGGGCCGGGATGCCAACAAATGAAACCATCGGCTCACCTGAATGCAGAAGAATATGGCCATATCTTATAACAAAACGATGCAAAGGAAAAATATATGCAGAGCTAAGAGATACGATTTCATTGGAAGACGCACTGAACTTAGCATCCTTTCTGGGAGAGCAGCTCCGCAACCTCCATCTCTTGCCTCAGCCACCTCTAAGCATCTCAACTTTCTCAGATATTAAACCAGAAATAGATATGCCTTTAACTAATGGTTGTATGGAGGCTGTTCAGGATAAATCAGAAGTTCCAGCTGAATGGAATATTTTCATCAGAACTCTAATCAGGAAGAAGAAGGATGTCTCAAGTCGCTTGAGTAAATGGGGAGATCCAATTCCTACCACACTGATCGAGAAAGTCGATGAATACTTACCAGATGATTTGGCCAAGTTGCTTCACATATTCGAGGATGAAAATGGCTTAAACAAGGTGGGTAAACTTTGTTCCTGGATACACTCAGATATCATGGATGATAATATTCACATGGAACCATGTGGTGCTAATTCTTGCCTGATTGAGAACACCAAAGACAATGGCCTGGTAACCAATGGTTCAGTGAATGGAAATGGCATCAGTGCAGGGACTAAATCATGGCGTCCTAGCCACATTCTTGATTTCAGTGATCTTTCTATAGGTGACCCCATTTATGATATAATACCCATATACTTGGATGTATTTAGAGGTGATACACGTCTCCTTAAGCAACTTTTAGAAAGTTATAAACTTCCTTTAGTGAGTGCAGAATCACAGAATAAGTCTGTTAAGGGTGGAGACAAGTTTGGACAGCTTTCCTACCACGCAATGTGCTACTGTATGCTGCATGACGAGAATGTATTGGGAGCCATTTTTAGTATCTGGGACGAACTCAAGACGGCGAAAACATGGGAAGAAGTTGAGCAAGTAGTGTGGGGAGAGTTGAATAATTACAAGGGGTTTCCCTTGGATCCTAAAATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
APH Cupin_8 F-box F-box-like JmjC

Protein Analysis

982

Amino Acids

111.64

Weight (kDa)

5.32

Isoelectric Point (pI)

43.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box-like PF12937 24 - 68 1.4e-09 F-box-like
F-box PF00646 25 - 62 1.8e-07 F-box domain
Cupin_8 PF13621 151 - 370 5.4e-19 Cupin-like domain
JmjC PF02373 255 - 368 4.4e-14 JmjC domain, hydroxylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000630)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G78280
fragaria_vesca FvH4_5g36800 FvH4_5g36800 FvH4_5g36800 FvH4_5g36800 FvH4_5g36800 FvH4_5g36800 FvH4_5g36800 FvH4_5g36800 FvH4_5g36800 FvH4_5g36800 FvH4_5g36800 FvH4_5g36800 FvH4_5g36800 FvH4_5g36800 FvH4_5g36800 FvH4_5g36800 FvH4_5g36800 FvH4_5g36800
malus_domestica MD08G1186800.v1.1 MD15G1372700.v1.1
prunus_persica Prupe.1G521300_v2.0.a1 Prupe.1G521300_v2.0.a1
pyrus_communis pycom08g16060 pycom15g33370
rosa_chinensis RchiOBHm_Chr2g0139391 RchiOBHm_Chr7g0242931 RchiOBHm_Chr7g0242951 RchiOBHm_Chr7g0243001 RchiOBHm_Chr7g0243011 RchiOBHm_Chr7g0243141 RchiOBHm_Chr7g0243151 RchiOBHm_Chr7g0243351
rosa_laevigata RLG00000000853 RLG00000000857 RLG00000000858 RLG00000000863 RLG00000000867
rosa_multiflora Rmu_co8457323.1_g000001 Rmu_sc0000083.1_g000017 Rmu_sc0000679.1_g000021 Rmu_sc0001036.1_g000029 Rmu_sc0001036.1_g000032 Rmu_sc0001176.1_g000015 Rmu_sc0001461.1_g000002 Rmu_sc0001461.1_g000003 Rmu_sc0001461.1_g000056 Rmu_sc0001461.1_g000057 Rmu_sc0001461.1_g000098 Rmu_sc0001461.1_g000105 Rmu_sc0004165.1_g000084 Rmu_sc0006141.1_g000004 Rmu_sc0033806.1_g000001
rosa_roxburghii Rroxscaffold_3G00222160 Rroxscaffold_3G00222220 Rroxscaffold_4G00316290
rosa_rugosa Rorug07G0318400 Rorug07G0318400 Rorug07G0318600
rosa_samantha Rh4CG066500 Rh5DG444300 Rh7AG473700 Rh7AG473900 Rh7AG474000 Rh7AG474600 Rh7AG474800 Rh7BG444500 Rh7BG444700 Rh7CG490700 Rh7CG490800 Rh7CG491000 Rh7DG458900 Rh7DG459000 Rh7DG459300
rosa_wichuraiana Rw7G038970 Rw7G039050 Rw7G039420 Rw7G039480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 2018, 2709
AarI CACCTGC 1 cut(s) 682
AasI GACNNNNNNGTC 2 cut(s) 68, 1664
AatII GACGTC 1 cut(s) 126
Acc36I ACCTGC 1 cut(s) 682
AccB1I GGYRCC 2 cut(s) 791, 1144
AccB7I CCANNNNNTGG 1 cut(s) 828
AccBSI CCGCTC 2 cut(s) 53, 220
AccII CGCG 2 cut(s) 23, 120
AciI CCGC 3 cut(s) 51, 218, 2116
AclWI GGATC 7 cut(s) 626, 639, 803, 1805, 2312, 2927, 2940
AcoI YGGCCR 5 cut(s) 44, 48, 847, 2006, 2373
AcuI CTGAAG 1 cut(s) 1784
AcyI GRCGYC 2 cut(s) 123, 2584
AdeI CACNNNGTG 1 cut(s) 893
AfaI GTAC 7 cut(s) 158, 270, 338, 885, 903, 1012, 1833
AfiI CCNNNNNNNGG 2 cut(s) 421, 828
AflII CTTAAG 1 cut(s) 2687
AflIII ACRYGT 3 cut(s) 21, 904, 2678
AgeI ACCGGT 1 cut(s) 457
AhlI ACTAGT 1 cut(s) 841
AjiI CACGTC 1 cut(s) 2681
AleI CACNNNNGTG 1 cut(s) 955
AloI GAACNNNNNNTCC 2 cut(s) 2078, 2110
Alw21I GWGCWC 2 cut(s) 571, 1430
Alw26I GTCTC 7 cut(s) 54, 583, 735, 1497, 2296, 2687, 2754
AlwI GGATC 7 cut(s) 626, 639, 803, 1805, 2312, 2927, 2940
AlwNI CAGNNNCTG 2 cut(s) 828, 1856
ApaI GGGCCC 1 cut(s) 865
ApeKI GCWGC 2 cut(s) 2110, 2806
ArsI GACNNNNNNTTYG 2 cut(s) 641, 673
AsiGI ACCGGT 1 cut(s) 457
Asp700I GAANNNNTTC 1 cut(s) 2255
AspS9I GGNCC 6 cut(s) 16, 215, 776, 861, 862, 1953
AsuC2I CCSGG 1 cut(s) 1957
AsuHPI GGTGA 4 cut(s) 935, 1977, 2636, 2684
AvaII GGWCC 1 cut(s) 215
BaeGI GKGCMC 1 cut(s) 865
BalI TGGCCA 2 cut(s) 2008, 2375
BamHI GGATCC 2 cut(s) 631, 2932
BanI GGYRCC 2 cut(s) 791, 1144
BanII GRGCYC 3 cut(s) 33, 865, 1430
BauI CACGAG 1 cut(s) 235
BbsI GAAGAC 3 cut(s) 251, 432, 2082
Bbv12I GWGCWC 2 cut(s) 571, 1430
BbvCI CCTCAGC 2 cut(s) 200, 2136
BbvI GCAGC 2 cut(s) 2122, 2793
BceAI ACGGC 5 cut(s) 264, 834, 1073, 1158, 2877
BciVI GTATCC 2 cut(s) 879, 2433
BcnI CCSGG 1 cut(s) 1957
BcoDI GTCTC 7 cut(s) 54, 583, 735, 1497, 2296, 2687, 2754
BcuI ACTAGT 1 cut(s) 841
BfaI CTAG 7 cut(s) 842, 890, 965, 1391, 1712, 1724, 2589
BfmI CTRYAG 2 cut(s) 953, 2619
BfrI CTTAAG 1 cut(s) 2687
BfuAI ACCTGC 1 cut(s) 682
BfuI GTATCC 2 cut(s) 879, 2433
BglI GCCNNNNNGGC 1 cut(s) 496
BisI GCNGC 3 cut(s) 51, 2111, 2807
BlsI GCNGC 3 cut(s) 52, 2112, 2808
Bme18I GGWCC 1 cut(s) 215
BmgBI CACGTC 1 cut(s) 2681
BmgT120I GGNCC 6 cut(s) 16, 215, 776, 861, 862, 1953
BmiI GGNNCC 8 cut(s) 633, 793, 863, 1077, 1146, 2478, 2830, 2934
BpiI GAAGAC 3 cut(s) 251, 432, 2082
BplI GAGNNNNNCTC 2 cut(s) 2097, 2129
BpmI CTGGAG 2 cut(s) 206, 1311
Bpu10I CCTNAGC 2 cut(s) 200, 2136
BpuEI CTTGAG 5 cut(s) 1672, 1829, 2278, 2323, 2840
BpuMI CCSGG 1 cut(s) 1957
BsaBI GATNNNNATC 2 cut(s) 630, 972
BsaHI GRCGYC 2 cut(s) 123, 2584
BsaJI CCNNGG 6 cut(s) 1022, 1350, 1351, 1421, 1835, 2928
BsaWI WCCGGW 1 cut(s) 457
Bsc4I CCNNNNNNNGG 2 cut(s) 421, 828
Bse118I RCCGGY 3 cut(s) 42, 46, 457
Bse1I ACTGG 4 cut(s) 223, 475, 517, 822
Bse3DI GCAATG 2 cut(s) 175, 2796
Bse8I GATNNNNATC 2 cut(s) 630, 972
BseDI CCNNGG 6 cut(s) 1022, 1350, 1351, 1421, 1835, 2928
BseJI GATNNNNATC 2 cut(s) 630, 972
BseLI CCNNNNNNNGG 2 cut(s) 421, 828
BseMI GCAATG 2 cut(s) 175, 2796
BseMII CTCAG 6 cut(s) 26, 214, 1878, 2150, 2178, 2460
BseNI ACTGG 4 cut(s) 223, 475, 517, 822
BseRI GAGGAG 1 cut(s) 105
BseSI GKGCMC 1 cut(s) 865
BseX3I CGGCCG 2 cut(s) 44, 48
BseXI GCAGC 2 cut(s) 2122, 2793
BseYI CCCAGC 1 cut(s) 1541
BsgI GTGCAG 2 cut(s) 2586, 2748
Bsh1236I CGCG 2 cut(s) 23, 120
Bsh1285I CGRYCG 2 cut(s) 47, 51
BshNI GGYRCC 2 cut(s) 791, 1144
BshTI ACCGGT 1 cut(s) 457
BsiEI CGRYCG 2 cut(s) 47, 51
BsiHKAI GWGCWC 2 cut(s) 571, 1430
BsiSI CCGG 4 cut(s) 43, 47, 458, 1956
BslFI GGGAC 2 cut(s) 2584, 2861
BslI CCNNNNNNNGG 2 cut(s) 421, 828
BsmAI GTCTC 7 cut(s) 54, 583, 735, 1497, 2296, 2687, 2754
BsmBI CGTCTC 1 cut(s) 2687
BsmFI GGGAC 2 cut(s) 2584, 2861
BsmI GAATGC 5 cut(s) 284, 1309, 1562, 1577, 1997
Bsp120I GGGCCC 1 cut(s) 861
Bsp1286I GDGCHC 4 cut(s) 33, 571, 865, 1430
Bsp1407I TGTACA 2 cut(s) 156, 336
Bsp19I CCATGG 1 cut(s) 1835
Bsp68I TCGCGA 1 cut(s) 120
BspACI CCGC 3 cut(s) 51, 218, 2116
BspCNI CTCAG 6 cut(s) 25, 213, 1879, 2149, 2177, 2459
BspFNI CGCG 2 cut(s) 23, 120
BspLI GGNNCC 8 cut(s) 633, 793, 863, 1077, 1146, 2478, 2830, 2934
BspMAI CTGCAG 1 cut(s) 957
BspMI ACCTGC 1 cut(s) 682
BspPI GGATC 7 cut(s) 626, 639, 803, 1805, 2312, 2927, 2940
BspQI GCTCTTC 1 cut(s) 168
BspT107I GGYRCC 2 cut(s) 791, 1144
BspTI CTTAAG 1 cut(s) 2687
BsrBI CCGCTC 2 cut(s) 53, 220
BsrDI GCAATG 2 cut(s) 175, 2796
BsrFI RCCGGY 3 cut(s) 42, 46, 457
BsrGI TGTACA 2 cut(s) 156, 336
BsrI ACTGG 4 cut(s) 223, 475, 517, 822
BssAI RCCGGY 3 cut(s) 42, 46, 457
BssECI CCNNGG 6 cut(s) 1022, 1350, 1351, 1421, 1835, 2928
BssNI GRCGYC 2 cut(s) 123, 2584
BssSI CACGAG 1 cut(s) 235
BssT1I CCWWGG 3 cut(s) 1421, 1835, 2928
Bst2BI CACGAG 1 cut(s) 235
Bst4CI ACNGT 6 cut(s) 695, 901, 1061, 1852, 1938, 2801
Bst6I CTCTTC 2 cut(s) 168, 874
BstACI GRCGYC 2 cut(s) 123, 2584
BstAFI CTTAAG 1 cut(s) 2687
BstAPI GCANNNNNTGC 1 cut(s) 1538
BstAUI TGTACA 2 cut(s) 156, 336
BstC8I GCNNGC 7 cut(s) 44, 48, 130, 139, 292, 492, 571
BstDSI CCRYGG 1 cut(s) 1835
BstEII GGTNACC 2 cut(s) 2530, 2624
BstFNI CGCG 2 cut(s) 23, 120
BstMAI GTCTC 7 cut(s) 54, 583, 735, 1497, 2296, 2687, 2754
BstMCI CGRYCG 2 cut(s) 47, 51
BstNSI RCATGY 5 cut(s) 269, 806, 908, 1196, 1777
BstPI GGTNACC 2 cut(s) 2530, 2624
BstSFI CTRYAG 2 cut(s) 953, 2619
BstSLI GKGCMC 1 cut(s) 865
BstUI CGCG 2 cut(s) 23, 120
BstV1I GCAGC 2 cut(s) 2122, 2793
BstV2I GAAGAC 3 cut(s) 251, 432, 2082
BstX2I RGATCY 3 cut(s) 631, 2317, 2932
BstXI CCANNNNNNTGG 5 cut(s) 659, 762, 1050, 2247, 2656
BstYI RGATCY 3 cut(s) 631, 2317, 2932
BstZI CGGCCG 2 cut(s) 44, 48
BsuI GTATCC 2 cut(s) 879, 2433
BtgI CCRYGG 1 cut(s) 1835
BtrI CACGTC 1 cut(s) 2681
BtsI GCAGTG 3 cut(s) 154, 950, 962
BtuMI TCGCGA 1 cut(s) 120
BveI ACCTGC 1 cut(s) 682
Cac8I GCNNGC 7 cut(s) 44, 48, 130, 139, 292, 492, 571
CaiI CAGNNNCTG 2 cut(s) 828, 1856
Cfr10I RCCGGY 3 cut(s) 42, 46, 457
Cfr13I GGNCC 6 cut(s) 16, 215, 776, 861, 862, 1953
CseI GACGC 2 cut(s) 2087, 2573
CsiI ACCWGGT 1 cut(s) 1147
Csp6I GTAC 7 cut(s) 157, 269, 337, 884, 902, 1011, 1832
CspAI ACCGGT 1 cut(s) 457
CspCI CAANNNNNGTGG 2 cut(s) 2876, 2911
CviQI GTAC 7 cut(s) 157, 269, 337, 884, 902, 1011, 1832
DraIII CACNNNGTG 1 cut(s) 893
DrdI GACNNNNNNGTC 2 cut(s) 68, 1664
DseDI GACNNNNNNGTC 2 cut(s) 68, 1664
EaeI YGGCCR 5 cut(s) 44, 48, 847, 2006, 2373
EagI CGGCCG 2 cut(s) 44, 48
Eam1104I CTCTTC 2 cut(s) 168, 874
EarI CTCTTC 2 cut(s) 168, 874
Ecl136II GAGCTC 1 cut(s) 1428
EclXI CGGCCG 2 cut(s) 44, 48
Eco130I CCWWGG 3 cut(s) 1421, 1835, 2928
Eco24I GRGCYC 3 cut(s) 33, 865, 1430
Eco32I GATATC 1 cut(s) 2452
Eco47I GGWCC 1 cut(s) 215
Eco52I CGGCCG 2 cut(s) 44, 48
Eco53kI GAGCTC 1 cut(s) 1428
Eco57I CTGAAG 1 cut(s) 1784
Eco91I GGTNACC 2 cut(s) 2530, 2624
EcoICRI GAGCTC 1 cut(s) 1428
EcoO109I RGGNCCY 1 cut(s) 862
EcoO65I GGTNACC 2 cut(s) 2530, 2624
EcoRI GAATTC 1 cut(s) 7
EcoRV GATATC 1 cut(s) 2452
EcoT14I CCWWGG 3 cut(s) 1421, 1835, 2928
EcoT38I GRGCYC 3 cut(s) 33, 865, 1430
ErhI CCWWGG 3 cut(s) 1421, 1835, 2928
Esp3I CGTCTC 1 cut(s) 2687
FalI AAGNNNNNCTT 2 cut(s) 165, 197
FaqI GGGAC 2 cut(s) 2584, 2861
FauNDI CATATG 1 cut(s) 274
Fnu4HI GCNGC 3 cut(s) 51, 2111, 2807
FriOI GRGCYC 3 cut(s) 33, 865, 1430
FseI GGCCGGCC 1 cut(s) 50
Fsp4HI GCNGC 3 cut(s) 51, 2111, 2807
FspBI CTAG 7 cut(s) 842, 890, 965, 1391, 1712, 1724, 2589
GluI GCNGC 3 cut(s) 51, 2111, 2807
GsaI CCCAGC 1 cut(s) 1545
GsuI CTGGAG 2 cut(s) 206, 1311
HapII CCGG 4 cut(s) 43, 47, 458, 1956
HgaI GACGC 2 cut(s) 2087, 2573
Hin1I GRCGYC 2 cut(s) 123, 2584
HincII GTYRAC 1 cut(s) 1753
HindII GTYRAC 1 cut(s) 1753
HindIII AAGCTT 2 cut(s) 1475, 1613
HinfI GANTC 7 cut(s) 69, 318, 1235, 1272, 1279, 1768, 2732
HpaII CCGG 4 cut(s) 43, 47, 458, 1956
HphI GGTGA 4 cut(s) 935, 1977, 2636, 2684
Hpy166II GTNNAC 4 cut(s) 910, 1011, 1753, 2426
Hpy8I GTNNAC 4 cut(s) 910, 1011, 1753, 2426
Hpy99I CGWCG 3 cut(s) 125, 128, 287
HpyAV CCTTC 6 cut(s) 83, 1193, 1510, 1679, 1944, 2278
HpyCH4III ACNGT 6 cut(s) 695, 901, 1061, 1852, 1938, 2801
HpyCH4IV ACGT 2 cut(s) 123, 2680
HpySE526I ACGT 2 cut(s) 123, 2680
Hsp92I GRCGYC 2 cut(s) 123, 2584
KroI GCCGGC 2 cut(s) 42, 46
KroNI GCCGGC 2 cut(s) 44, 48
LguI GCTCTTC 1 cut(s) 168
LmnI GCTCC 7 cut(s) 225, 262, 1425, 1520, 1926, 2118, 2828
Lsp1109I GCAGC 2 cut(s) 2122, 2793
MabI ACCWGGT 1 cut(s) 1147
MaeI CTAG 7 cut(s) 842, 890, 965, 1391, 1712, 1724, 2589
MaeII ACGT 2 cut(s) 123, 2680
MaeIII GTNAC 5 cut(s) 437, 895, 1090, 2530, 2624
MbiI CCGCTC 2 cut(s) 53, 220
MflI RGATCY 3 cut(s) 631, 2317, 2932
MhlI GDGCHC 4 cut(s) 33, 571, 865, 1430
MlsI TGGCCA 2 cut(s) 2008, 2375
MluI ACGCGT 1 cut(s) 21
MluNI TGGCCA 2 cut(s) 2008, 2375
MlyI GAGTC 3 cut(s) 78, 1273, 1281
Mox20I TGGCCA 2 cut(s) 2008, 2375
MroNI GCCGGC 2 cut(s) 42, 46
MroXI GAANNNNTTC 1 cut(s) 2255
MscI TGGCCA 2 cut(s) 2008, 2375
MslI CAYNNNNRTG 4 cut(s) 277, 760, 955, 1990
Msp20I TGGCCA 2 cut(s) 2008, 2375
MspA1I CMGCKG 1 cut(s) 2243
MspCI CTTAAG 1 cut(s) 2687
MspI CCGG 4 cut(s) 43, 47, 458, 1956
Mva1269I GAATGC 5 cut(s) 284, 1309, 1562, 1577, 1997
MvnI CGCG 2 cut(s) 23, 120
NaeI GCCGGC 2 cut(s) 44, 48
NciI CCSGG 1 cut(s) 1957
NcoI CCATGG 1 cut(s) 1835
NdeI CATATG 1 cut(s) 274
NgoMIV GCCGGC 2 cut(s) 42, 46
NlaIV GGNNCC 8 cut(s) 633, 793, 863, 1077, 1146, 2478, 2830, 2934
NmuCI GTSAC 3 cut(s) 437, 895, 2624
NruI TCGCGA 1 cut(s) 120
NspI RCATGY 5 cut(s) 269, 806, 908, 1196, 1777
OliI CACNNNNGTG 1 cut(s) 955
PaqCI CACCTGC 1 cut(s) 682
PasI CCCWGGG 1 cut(s) 1351
PciI ACATGT 1 cut(s) 904
PciSI GCTCTTC 1 cut(s) 168
PcsI WCGNNNNNNNCGW 1 cut(s) 2346
PctI GAATGC 5 cut(s) 284, 1309, 1562, 1577, 1997
PdiI GCCGGC 2 cut(s) 44, 48
PdmI GAANNNNTTC 1 cut(s) 2255
PfeI GAWTC 4 cut(s) 318, 1235, 1768, 2732
PflMI CCANNNNNTGG 1 cut(s) 828
PfoI TCCNGGA 2 cut(s) 99, 2435
PinAI ACCGGT 1 cut(s) 457
PkrI GCNGC 3 cut(s) 52, 2112, 2808
PleI GAGTC 3 cut(s) 77, 1273, 1280
PpsI GAGTC 3 cut(s) 77, 1273, 1280
PscI ACATGT 1 cut(s) 904
PsiI TTATAA 2 cut(s) 2018, 2709
Psp124BI GAGCTC 1 cut(s) 1430
PspEI GGTNACC 2 cut(s) 2530, 2624
PspFI CCCAGC 1 cut(s) 1541
PspN4I GGNNCC 8 cut(s) 633, 793, 863, 1077, 1146, 2478, 2830, 2934
PspOMI GGGCCC 1 cut(s) 861
PspPI GGNCC 6 cut(s) 16, 215, 776, 861, 862, 1953
PsrI GAACNNNNNNTAC 2 cut(s) 2416, 2448
PstI CTGCAG 1 cut(s) 957
PstNI CAGNNNCTG 2 cut(s) 828, 1856
PsuI RGATCY 3 cut(s) 631, 2317, 2932
PvuII CAGCTG 1 cut(s) 2243
RigI GGCCGGCC 1 cut(s) 50
RruI TCGCGA 1 cut(s) 120
RsaI GTAC 7 cut(s) 158, 270, 338, 885, 903, 1012, 1833
RsaNI GTAC 7 cut(s) 157, 269, 337, 884, 902, 1011, 1832
RseI CAYNNNNRTG 4 cut(s) 277, 760, 955, 1990
SacI GAGCTC 1 cut(s) 1430
SapI GCTCTTC 1 cut(s) 168
SatI GCNGC 3 cut(s) 51, 2111, 2807
Sau96I GGNCC 6 cut(s) 16, 215, 776, 861, 862, 1953
SchI GAGTC 3 cut(s) 78, 1273, 1281
SduI GDGCHC 4 cut(s) 33, 571, 865, 1430
SexAI ACCWGGT 1 cut(s) 1147
SfcI CTRYAG 2 cut(s) 953, 2619
SinI GGWCC 1 cut(s) 215
SmiMI CAYNNNNRTG 4 cut(s) 277, 760, 955, 1990
SmlI CTYRAG 6 cut(s) 1651, 1808, 2293, 2302, 2687, 2855
SmoI CTYRAG 6 cut(s) 1651, 1808, 2293, 2302, 2687, 2855
SpeI ACTAGT 1 cut(s) 841
SsiI CCGC 3 cut(s) 51, 218, 2116
SspI AATATT 2 cut(s) 2254, 2467
SspMI CTAG 7 cut(s) 842, 890, 965, 1391, 1712, 1724, 2589
SstI GAGCTC 1 cut(s) 1430
StyI CCWWGG 3 cut(s) 1421, 1835, 2928
TaaI ACNGT 6 cut(s) 695, 901, 1061, 1852, 1938, 2801
TaiI ACGT 2 cut(s) 126, 2683
TaqI TCGA 5 cut(s) 936, 1284, 2340, 2349, 2394
TatI WGTACW 2 cut(s) 156, 336
TauI GCSGC 1 cut(s) 53
TfiI GAWTC 4 cut(s) 318, 1235, 1768, 2732
TseFI GTSAC 3 cut(s) 437, 895, 2624
TseI GCWGC 2 cut(s) 2110, 2806
Tsp45I GTSAC 3 cut(s) 437, 895, 2624
TspDTI ATGAA 9 cut(s) 186, 291, 594, 599, 1986, 2058, 2248, 2367, 2415
Van91I CCANNNNNTGG 1 cut(s) 828
Vha464I CTTAAG 1 cut(s) 2687
VpaK11BI GGWCC 1 cut(s) 215
XbaI TCTAGA 1 cut(s) 1390
XceI RCATGY 5 cut(s) 269, 806, 908, 1196, 1777
XcmI CCANNNNNNNNNTGG 2 cut(s) 759, 1050
XmnI GAANNNNTTC 1 cut(s) 2255
XspI CTAG 7 cut(s) 842, 890, 965, 1391, 1712, 1724, 2589
ZraI GACGTC 1 cut(s) 124
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.