RchiOBHm_Chr7g0243351

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0000630
Physical Location & Seq
Reverse (-)
69624245 .. 69632043
7799 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ21809

Sequence Viewer

Length: 2973 bp
ATGGAGATTTCCGAAGCCCCTCCCTTCCGGCCAAGAGACCGCCGGCCGGACGCCCTCGGAAGCTTCCTAGTCCTCCCCGACGAGCTCATCTGTGCCGTCTTGGAATTGCTCTCTCCTCGCGACGTTGCTCGCCTCTCATGCGTCAGCAGTGTGATGTATATATTTTGCAATGAAGAGCCACTCTGGTTGGGTTTATGCCTCAACACACTGAATGGTCCGCTCCAGTACAAAGGATCGTGGAAGAAGACGGTGCTGCATTTGGAGAATGTGTCGTATGAACGCGACGAGGCTGGCAGAAAACCACTGAGTTTTGATGGATTCGACTCTTTGTTCTTGTACAGAAGATTATACAGGTGTCATACCACATTGGATGGTTTTTCTTTTGACAATGGGAATGTGGAAAGAAAGGACAAGATCACAGTGGAAGAGTTTTCTTGTGACTATGATGGAAAGAAACCGGTTTTGCTCACTGGATTAGCTGATGCATGGCCTGCAAGGCCTACATGGACACTTGACCACTTATTGCAGAATTATGGAGATACAGCTTTCAAGATTTCTCAAAGGAGCTCACGTAAAATCTCAATGACATTCAAGGATTATGTTTCATATATGAAAGTTCAGCATGATGAGGATCCGCTTTATATCTTTGATCACAAGTTTGGCGAGGTTGAACCTGGCTTGTTGAAAGATTACAGTGTACCTTATCTATTTCAAGAGGACTTTTTTGATGTTTTGGACAAAGATAAGCGACCTCCATTTAGATGGCTCATTATTGGTCCCCAGAGGTCTGGTGCTTCTTGGCATGTTGATCCAGCTCTGACCAGTGCGTGGAATACGCTTCTATGTGGTCGTAAAAGGTGGGCCTTGTATCCTCCAGGAAGAGTACCTATAGGTGTCACAGTACATGTAAATGAAGAAGACGGTGATGTCAATATTGAAACTCCAACTTCGTTGCAGTGGTGGCTAGATTTTTATCCACTTCTTGCTGATGAAGACAAGCCCATAGAGTGCACCCAAATGCCGGGGGAGACGATTTTTGTTCCTAGTGGATGGTGGCACAGTGTCCTAAATCTTGAACCATCTATTGCAGTTACCCAGAACTTTGTAAATTCTAAGAACTTTGAATTTGTGTGCTTGGATATGGCACCTGGTTATCGTCATAAGGGAGTTTGCCGTGCTGGATTACTTGCAGATGATGAAGGCAGTATTGAGGATTCCACAAATCATATACTCTATGATAATGATGATTATAATTCCTCAGATATGACTAGGAAAGTAAAAAGAGTCAGAACTCTCAAACCTGGAGAATATCCAAGTACTGAAAGAACTAGTAATGGCGCTCAGGCTTTTTCTTATGATGTAAATGTCTTAGCCATGTATCTGGATGAAGAGAGAGATCATTACAACTCTCCTTGGAGCTCAGGCAATTGCATAGGGCAACGAGAAATGAGAGAATGGTTATTCAAGCTTTGGGTTGGAAAACCAGGAATGAGAGACTTAATCTGGAAGGGAGCATGTCTTGCACTAAATGCGAGCAAATGGTCAGAATCCCTGGCAGAAATTTGTGCCTTCCACAAATTGCCTTCTCCAACAGATGATGAGAGGCTTCCTGTTGGCACGGGTAGCAATCCTGTTTATCTCATGAGCAACTGTGTAATCAAAATTTTTGTTGAGGAAGGACTGGAAACGTCACTTTATGGATTAGGTGCTGAGCTTGAGTTCTACAGTCTCCTAGGCAAAGTCAAATCCCCTCTGAAAAATCACATACCTGACGTTTTGGCAAGTGGGATCATTTATCTTGAAAATGGAACTTATAAAATTGTACCTTGGGATGGCAACGGCGTTCCTGATGTGATTGCTAAGTGCAATTTTATTCCGGAGAAGGTCAAAGGAGATGTTTCTCCTTTTGGTGTATGGAGGAAGAAACAATTTGAATACAGAAAAGCTGGTGTCTCAACAAATGAATCCATCAGTTCAGCTGAATATACAAGGATATGGCCATATCTCATAACAAAACGATGCAAAGGAAAAATATATGCAGAGTTAAGAGATACTGTGTCACGGGAAGATGAATTGAACTTAGCTTCCTTCTTGGGAGAGCAGCTCCGTAACCTCCATCTCTTGCCCCATCCACCTCTTAATATCTCAACTTTCTCAGATATTGAACAGGAATCAGATTTTCTCTTCACCAGTGGTAGTATAGAAGCTGTTCCCAATAAATCAAACATTCCTGCTGAATGGGATATATTCATTAGAAGTCTATCCAAGAAGAAGAAGGATGTCTCAAGTCGCTTGATTAAATGGGGAGATCCAATTCCTAGCACACTCATAGAGAAAGTTGACAAATACATCCCAGATGATTTTGCCAAGTTTCTGTACATATTCAAGGATGAAAATGGTGTAAGCAATGTTGGTAAAAGTTGTTCGTGGATACACTCTGATATCATGGATGACAACATTCACATGGAACTATGTGGTTTTAATTCTTGCGTCATTGGCAATGCCAAAAACACTTGCCTGGTAAAGAATGGTTCCTTGAATGTTGATGGTGACCGTGCAGAGAGGAGAACATGGTGCCCTAGCCACATTCTTGATTTCAGTAATCTTTCTATAGGTGATCCCATCTATGATTTGATACCCTTATACTTGGATATATTCAGAGGAGATTGCTGCCTCCTTAAGAGATTTTTAGATAGTTATAAACTTCCTTTAGTAAGACAAGCATCACCTCATGAGTATATTGAGGGTGGCGACAAGTTTGGACGACTTTCATACCATGCCATGTGCTACTGTATTCTACATGAAGAGAATGTCCTGGGAGCAATTTTTAGTCTCTGGGACGAACTCAAGATGGCAAAATCATGGGAAGAAGTTGAACAGGTCGTATGGGGCGAGCTAAACAACTACAAAGGCTTTACTGGATCATCCACATCTCTCTCTTTGTTGGAGCCGGAAACTCCCTCAACATTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
APH Cupin_8 F-box F-box-like JmjC

Protein Analysis

990

Amino Acids

112.73

Weight (kDa)

5.26

Isoelectric Point (pI)

43.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box-like PF12937 23 - 67 4.3e-07 F-box-like
F-box PF00646 23 - 62 6.4e-06 F-box domain
Cupin_8 PF13621 150 - 371 2.8e-19 Cupin-like domain
JmjC PF02373 255 - 368 3.5e-15 JmjC domain, hydroxylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000630)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G78280
fragaria_vesca FvH4_5g36800 FvH4_5g36800 FvH4_5g36800 FvH4_5g36800 FvH4_5g36800 FvH4_5g36800 FvH4_5g36800 FvH4_5g36800 FvH4_5g36800 FvH4_5g36800 FvH4_5g36800 FvH4_5g36800 FvH4_5g36800 FvH4_5g36800 FvH4_5g36800 FvH4_5g36800 FvH4_5g36800 FvH4_5g36800
malus_domestica MD08G1186800.v1.1 MD15G1372700.v1.1
prunus_persica Prupe.1G521300_v2.0.a1 Prupe.1G521300_v2.0.a1
pyrus_communis pycom08g16060 pycom15g33370
rosa_chinensis RchiOBHm_Chr2g0139391 RchiOBHm_Chr7g0242931 RchiOBHm_Chr7g0242951 RchiOBHm_Chr7g0243001 RchiOBHm_Chr7g0243011 RchiOBHm_Chr7g0243141 RchiOBHm_Chr7g0243151 RchiOBHm_Chr7g0243351
rosa_laevigata RLG00000000853 RLG00000000857 RLG00000000858 RLG00000000863 RLG00000000867
rosa_multiflora Rmu_co8457323.1_g000001 Rmu_sc0000083.1_g000017 Rmu_sc0000679.1_g000021 Rmu_sc0001036.1_g000029 Rmu_sc0001036.1_g000032 Rmu_sc0001176.1_g000015 Rmu_sc0001461.1_g000002 Rmu_sc0001461.1_g000003 Rmu_sc0001461.1_g000056 Rmu_sc0001461.1_g000057 Rmu_sc0001461.1_g000098 Rmu_sc0001461.1_g000105 Rmu_sc0004165.1_g000084 Rmu_sc0006141.1_g000004 Rmu_sc0033806.1_g000001
rosa_roxburghii Rroxscaffold_3G00222160 Rroxscaffold_3G00222220 Rroxscaffold_4G00316290
rosa_rugosa Rorug07G0318400 Rorug07G0318400 Rorug07G0318600
rosa_samantha Rh4CG066500 Rh5DG444300 Rh7AG473700 Rh7AG473900 Rh7AG474000 Rh7AG474600 Rh7AG474800 Rh7BG444500 Rh7BG444700 Rh7CG490700 Rh7CG490800 Rh7CG491000 Rh7DG458900 Rh7DG459000 Rh7DG459300
rosa_wichuraiana Rw7G038970 Rw7G039050 Rw7G039420 Rw7G039480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 1251, 1815, 2700
AasI GACNNNNNNGTC 1 cut(s) 926
AccB1I GGYRCC 2 cut(s) 1144, 2574
AccB7I CCANNNNNTGG 1 cut(s) 828
AccBSI CCGCTC 1 cut(s) 220
AccII CGCG 2 cut(s) 120, 282
AccIII TCCGGA 1 cut(s) 1876
AciI CCGC 3 cut(s) 40, 218, 635
AclWI GGATC 8 cut(s) 241, 626, 639, 803, 1796, 2303, 2612, 2929
AcoI YGGCCR 3 cut(s) 29, 44, 1997
AcsI RAATTY 4 cut(s) 1108, 1124, 1560, 1662
AcyI GRCGYC 1 cut(s) 51
AfaI GTAC 8 cut(s) 227, 338, 699, 885, 903, 1318, 1824, 2378
AfiI CCNNNNNNNGG 4 cut(s) 46, 828, 1021, 1832
AflII CTTAAG 1 cut(s) 2678
AflIII ACRYGT 1 cut(s) 904
AgeI ACCGGT 1 cut(s) 457
AhlI ACTAGT 1 cut(s) 1328
AjnI CCWGG 8 cut(s) 673, 874, 1147, 1300, 1483, 1551, 2517, 2814
AloI GAACNNNNNNTCC 2 cut(s) 2069, 2101
Alw21I GWGCWC 4 cut(s) 87, 569, 1013, 1421
Alw26I GTCTC 7 cut(s) 30, 1022, 1488, 1733, 1957, 2287, 2837
Alw44I GTGCAC 1 cut(s) 1009
AlwI GGATC 8 cut(s) 241, 626, 639, 803, 1796, 2303, 2612, 2929
Aor13HI TCCGGA 1 cut(s) 1876
AoxI GGCC 6 cut(s) 29, 44, 488, 497, 861, 1997
ApaLI GTGCAC 1 cut(s) 1009
ApeKI GCWGC 3 cut(s) 253, 2101, 2670
ApoI RAATTY 4 cut(s) 1108, 1124, 1560, 1662
AsiGI ACCGGT 1 cut(s) 457
Asp700I GAANNNNTTC 1 cut(s) 2208
AspA2I CCTAGG 1 cut(s) 1732
AspLEI GCGC 1 cut(s) 1340
AspS9I GGNCC 3 cut(s) 215, 776, 861
AsuC2I CCSGG 1 cut(s) 1023
AsuHPI GGTGA 5 cut(s) 935, 2179, 2561, 2627, 2718
AvaII GGWCC 2 cut(s) 215, 776
AvrII CCTAGG 1 cut(s) 1732
BaeGI GKGCMC 2 cut(s) 1013, 2579
BalI TGGCCA 1 cut(s) 1999
BamHI GGATCC 1 cut(s) 631
BanI GGYRCC 2 cut(s) 1144, 2574
BanII GRGCYC 3 cut(s) 87, 569, 1421
BbsI GAAGAC 3 cut(s) 251, 924, 999
Bbv12I GWGCWC 4 cut(s) 87, 569, 1013, 1421
BbvI GCAGC 3 cut(s) 240, 2113, 2657
BceAI ACGGC 3 cut(s) 80, 1158, 1855
BciT130I CCWGG 8 cut(s) 675, 876, 1149, 1302, 1485, 1553, 2519, 2816
BciVI GTATCC 2 cut(s) 879, 2424
BclI TGATCA 1 cut(s) 649
BcnI CCSGG 1 cut(s) 1023
BcoDI GTCTC 7 cut(s) 30, 1022, 1488, 1733, 1957, 2287, 2837
BcuI ACTAGT 1 cut(s) 1328
BfaI CTAG 8 cut(s) 68, 965, 1044, 1269, 1329, 1733, 2319, 2580
BfmI CTRYAG 3 cut(s) 888, 1723, 2610
BfoI RGCGCY 1 cut(s) 1341
BfrI CTTAAG 1 cut(s) 2678
BfuI GTATCC 2 cut(s) 879, 2424
BglI GCCNNNNNGGC 1 cut(s) 496
BisI GCNGC 3 cut(s) 254, 2102, 2671
BlnI CCTAGG 1 cut(s) 1732
BlpI GCTNAGC 1 cut(s) 1710
BlsI GCNGC 3 cut(s) 255, 2103, 2672
BmcAI AGTACT 1 cut(s) 1318
Bme1390I CCNGG 9 cut(s) 675, 876, 1023, 1149, 1302, 1485, 1553, 2519, 2816
Bme18I GGWCC 2 cut(s) 215, 776
BmgT120I GGNCC 3 cut(s) 215, 776, 861
BmiI GGNNCC 6 cut(s) 633, 778, 1146, 2533, 2576, 2949
BmrFI CCNGG 9 cut(s) 675, 876, 1023, 1149, 1302, 1485, 1553, 2519, 2816
BmsI GCATC 3 cut(s) 472, 2009, 2732
BpiI GAAGAC 3 cut(s) 251, 924, 999
BplI GAGNNNNNCTC 2 cut(s) 2088, 2120
BpmI CTGGAG 3 cut(s) 206, 858, 1323
Bpu10I CCTNAGC 2 cut(s) 1341, 1420
Bpu1102I GCTNAGC 1 cut(s) 1710
BpuEI CTTGAG 3 cut(s) 1736, 2269, 2831
BpuMI CCSGG 1 cut(s) 1023
BsaAI YACGTR 1 cut(s) 572
BsaBI GATNNNNATC 2 cut(s) 630, 972
BsaHI GRCGYC 1 cut(s) 51
BsaI GGTCTC 1 cut(s) 30
BsaJI CCNNGG 7 cut(s) 55, 1022, 1412, 1551, 1732, 1826, 2815
BsaWI WCCGGW 2 cut(s) 457, 1876
BsaXI ACNNNNNCTCC 4 cut(s) 254, 284, 556, 586
Bsc4I CCNNNNNNNGG 4 cut(s) 46, 828, 1021, 1832
Bse118I RCCGGY 2 cut(s) 42, 457
Bse1I ACTGG 6 cut(s) 223, 475, 822, 1686, 2190, 2923
Bse3DI GCAATG 3 cut(s) 175, 2413, 2506
Bse8I GATNNNNATC 2 cut(s) 630, 972
BseAI TCCGGA 1 cut(s) 1876
BseBI CCWGG 8 cut(s) 675, 876, 1149, 1302, 1485, 1553, 2519, 2816
BseDI CCNNGG 7 cut(s) 55, 1022, 1412, 1551, 1732, 1826, 2815
BseJI GATNNNNATC 2 cut(s) 630, 972
BseLI CCNNNNNNNGG 4 cut(s) 46, 828, 1021, 1832
BseMI GCAATG 3 cut(s) 175, 2413, 2506
BseMII CTCAG 6 cut(s) 296, 1272, 1355, 1434, 1701, 2169
BseNI ACTGG 6 cut(s) 223, 475, 822, 1686, 2190, 2923
BseRI GAGGAG 3 cut(s) 105, 2578, 2676
BseSI GKGCMC 2 cut(s) 1013, 2579
BseX3I CGGCCG 1 cut(s) 44
BseXI GCAGC 3 cut(s) 240, 2113, 2657
BsgI GTGCAG 1 cut(s) 2577
Bsh1236I CGCG 2 cut(s) 120, 282
Bsh1285I CGRYCG 1 cut(s) 47
BshFI GGCC 6 cut(s) 31, 46, 490, 499, 863, 1999
BshNI GGYRCC 2 cut(s) 1144, 2574
BshTI ACCGGT 1 cut(s) 457
BsiEI CGRYCG 1 cut(s) 47
BsiHKAI GWGCWC 4 cut(s) 87, 569, 1013, 1421
BsiSI CCGG 7 cut(s) 28, 43, 47, 458, 1022, 1877, 2951
BslFI GGGAC 2 cut(s) 762, 2852
BslI CCNNNNNNNGG 4 cut(s) 46, 828, 1021, 1832
BsmAI GTCTC 7 cut(s) 30, 1022, 1488, 1733, 1957, 2287, 2837
BsmBI CGTCTC 1 cut(s) 1022
BsmFI GGGAC 2 cut(s) 762, 2852
BsnI GGCC 6 cut(s) 31, 46, 490, 499, 863, 1999
Bso31I GGTCTC 1 cut(s) 30
Bsp1286I GDGCHC 5 cut(s) 87, 569, 1013, 1421, 2579
Bsp13I TCCGGA 1 cut(s) 1876
Bsp1407I TGTACA 2 cut(s) 336, 2376
Bsp1720I GCTNAGC 1 cut(s) 1710
Bsp68I TCGCGA 1 cut(s) 120
BspACI CCGC 3 cut(s) 40, 218, 635
BspANI GGCC 6 cut(s) 31, 46, 490, 499, 863, 1999
BspCNI CTCAG 6 cut(s) 297, 1271, 1354, 1433, 1702, 2168
BspEI TCCGGA 1 cut(s) 1876
BspFNI CGCG 2 cut(s) 120, 282
BspHI TCATGA 2 cut(s) 1641, 2731
BspLI GGNNCC 6 cut(s) 633, 778, 1146, 2533, 2576, 2949
BspPI GGATC 8 cut(s) 241, 626, 639, 803, 1796, 2303, 2612, 2929
BspQI GCTCTTC 1 cut(s) 168
BspT107I GGYRCC 2 cut(s) 1144, 2574
BspTI CTTAAG 1 cut(s) 2678
BspTNI GGTCTC 1 cut(s) 30
BsrBI CCGCTC 1 cut(s) 220
BsrDI GCAATG 3 cut(s) 175, 2413, 2506
BsrFI RCCGGY 2 cut(s) 42, 457
BsrGI TGTACA 2 cut(s) 336, 2376
BsrI ACTGG 6 cut(s) 223, 475, 822, 1686, 2190, 2923
BssAI RCCGGY 2 cut(s) 42, 457
BssECI CCNNGG 7 cut(s) 55, 1022, 1412, 1551, 1732, 1826, 2815
BssNI GRCGYC 1 cut(s) 51
BssT1I CCWWGG 3 cut(s) 1412, 1732, 1826
Bst2UI CCWGG 8 cut(s) 675, 876, 1149, 1302, 1485, 1553, 2519, 2816
Bst6I CTCTTC 6 cut(s) 168, 420, 874, 1383, 2189, 2799
BstACI GRCGYC 1 cut(s) 51
BstAFI CTTAAG 1 cut(s) 2678
BstAPI GCANNNNNTGC 3 cut(s) 491, 1520, 1529
BstAUI TGTACA 2 cut(s) 336, 2376
BstBAI YACGTR 1 cut(s) 572
BstC8I GCNNGC 6 cut(s) 44, 130, 292, 492, 1534, 2894
BstEII GGTNACC 1 cut(s) 2549
BstFNI CGCG 2 cut(s) 120, 282
BstH2I RGCGCY 1 cut(s) 1341
BstHHI GCGC 1 cut(s) 1340
BstMAI GTCTC 7 cut(s) 30, 1022, 1488, 1733, 1957, 2287, 2837
BstMCI CGRYCG 1 cut(s) 47
BstMWI GCNNNNNNNGC 8 cut(s) 138, 491, 496, 961, 1520, 1529, 1623, 2496
BstNI CCWGG 8 cut(s) 675, 876, 1149, 1302, 1485, 1553, 2519, 2816
BstNSI RCATGY 3 cut(s) 806, 908, 1518
BstPI GGTNACC 1 cut(s) 2549
BstSCI CCNGG 9 cut(s) 673, 874, 1021, 1147, 1300, 1483, 1551, 2517, 2814
BstSFI CTRYAG 3 cut(s) 888, 1723, 2610
BstSLI GKGCMC 2 cut(s) 1013, 2579
BstUI CGCG 2 cut(s) 120, 282
BstV1I GCAGC 3 cut(s) 240, 2113, 2657
BstV2I GAAGAC 3 cut(s) 251, 924, 999
BstX2I RGATCY 2 cut(s) 631, 2308
BstXI CCANNNNNNTGG 3 cut(s) 762, 788, 1381
BstYI RGATCY 2 cut(s) 631, 2308
BstZI CGGCCG 1 cut(s) 44
BsuI GTATCC 2 cut(s) 879, 2424
BsuRI GGCC 6 cut(s) 31, 46, 490, 499, 863, 1999
BtsI GCAGTG 2 cut(s) 154, 962
BtuMI TCGCGA 1 cut(s) 120
Cac8I GCNNGC 6 cut(s) 44, 130, 292, 492, 1534, 2894
CciI TCATGA 2 cut(s) 1641, 2731
CfoI GCGC 1 cut(s) 1340
Cfr10I RCCGGY 2 cut(s) 42, 457
Cfr13I GGNCC 3 cut(s) 215, 776, 861
CseI GACGC 3 cut(s) 59, 130, 2479
CsiI ACCWGGT 1 cut(s) 1147
Csp6I GTAC 8 cut(s) 226, 337, 698, 884, 902, 1317, 1823, 2377
CspAI ACCGGT 1 cut(s) 457
CspCI CAANNNNNGTGG 2 cut(s) 168, 203
CviQI GTAC 8 cut(s) 226, 337, 698, 884, 902, 1317, 1823, 2377
DrdI GACNNNNNNGTC 1 cut(s) 926
DseDI GACNNNNNNGTC 1 cut(s) 926
EaeI YGGCCR 3 cut(s) 29, 44, 1997
EagI CGGCCG 1 cut(s) 44
Eam1104I CTCTTC 6 cut(s) 168, 420, 874, 1383, 2189, 2799
EarI CTCTTC 6 cut(s) 168, 420, 874, 1383, 2189, 2799
Ecl136II GAGCTC 3 cut(s) 85, 567, 1419
EclXI CGGCCG 1 cut(s) 44
Eco130I CCWWGG 3 cut(s) 1412, 1732, 1826
Eco147I AGGCCT 1 cut(s) 499
Eco24I GRGCYC 3 cut(s) 87, 569, 1421
Eco31I GGTCTC 1 cut(s) 30
Eco32I GATATC 1 cut(s) 2443
Eco47I GGWCC 2 cut(s) 215, 776
Eco52I CGGCCG 1 cut(s) 44
Eco53kI GAGCTC 3 cut(s) 85, 567, 1419
Eco91I GGTNACC 1 cut(s) 2549
EcoICRI GAGCTC 3 cut(s) 85, 567, 1419
EcoO65I GGTNACC 1 cut(s) 2549
EcoRII CCWGG 8 cut(s) 673, 874, 1147, 1300, 1483, 1551, 2517, 2814
EcoRV GATATC 1 cut(s) 2443
EcoT14I CCWWGG 3 cut(s) 1412, 1732, 1826
EcoT22I ATGCAT 1 cut(s) 487
EcoT38I GRGCYC 3 cut(s) 87, 569, 1421
ErhI CCWWGG 3 cut(s) 1412, 1732, 1826
Esp3I CGTCTC 1 cut(s) 1022
FaqI GGGAC 2 cut(s) 762, 2852
FbaI TGATCA 1 cut(s) 649
Fnu4HI GCNGC 3 cut(s) 254, 2102, 2671
FriOI GRGCYC 3 cut(s) 87, 569, 1421
Fsp4HI GCNGC 3 cut(s) 254, 2102, 2671
FspBI CTAG 8 cut(s) 68, 965, 1044, 1269, 1329, 1733, 2319, 2580
GlaI GCGC 1 cut(s) 1339
GluI GCNGC 3 cut(s) 254, 2102, 2671
GsuI CTGGAG 3 cut(s) 206, 858, 1323
HaeII RGCGCY 1 cut(s) 1341
HaeIII GGCC 6 cut(s) 31, 46, 490, 499, 863, 1999
HapII CCGG 7 cut(s) 28, 43, 47, 458, 1022, 1877, 2951
HgaI GACGC 3 cut(s) 59, 130, 2479
HhaI GCGC 1 cut(s) 1340
Hin1I GRCGYC 1 cut(s) 51
Hin6I GCGC 1 cut(s) 1338
HinP1I GCGC 1 cut(s) 1338
HincII GTYRAC 1 cut(s) 2341
HindII GTYRAC 1 cut(s) 2341
HindIII AAGCTT 2 cut(s) 61, 1466
HinfI GANTC 7 cut(s) 318, 323, 1214, 1284, 1547, 1964, 2171
HpaII CCGG 7 cut(s) 28, 43, 47, 458, 1022, 1877, 2951
HphI GGTGA 5 cut(s) 935, 2179, 2561, 2627, 2718
Hpy166II GTNNAC 3 cut(s) 698, 1011, 2341
Hpy8I GTNNAC 3 cut(s) 698, 1011, 2341
Hpy99I CGWCG 3 cut(s) 83, 125, 287
HpyAV CCTTC 9 cut(s) 34, 1193, 1501, 1579, 1593, 1670, 1876, 2098, 2269
HpyCH4IV ACGT 4 cut(s) 123, 571, 1688, 1773
HpyF10VI GCNNNNNNNGC 8 cut(s) 138, 491, 496, 961, 1520, 1529, 1623, 2496
HpySE526I ACGT 4 cut(s) 123, 571, 1688, 1773
Hsp92I GRCGYC 1 cut(s) 51
HspAI GCGC 1 cut(s) 1338
Kpn2I TCCGGA 1 cut(s) 1876
KroI GCCGGC 1 cut(s) 42
KroNI GCCGGC 1 cut(s) 44
Ksp22I TGATCA 1 cut(s) 649
LguI GCTCTTC 1 cut(s) 168
LmnI GCTCC 7 cut(s) 225, 564, 1416, 1511, 2109, 2819, 2947
Lsp1109I GCAGC 3 cut(s) 240, 2113, 2657
LweI GCATC 3 cut(s) 472, 2009, 2732
MabI ACCWGGT 1 cut(s) 1147
MaeI CTAG 8 cut(s) 68, 965, 1044, 1269, 1329, 1733, 2319, 2580
MaeII ACGT 4 cut(s) 123, 571, 1688, 1773
MaeIII GTNAC 7 cut(s) 437, 895, 1090, 1689, 2058, 2108, 2549
MbiI CCGCTC 1 cut(s) 220
MfeI CAATTG 1 cut(s) 1426
MflI RGATCY 2 cut(s) 631, 2308
MhlI GDGCHC 5 cut(s) 87, 569, 1013, 1421, 2579
MlsI TGGCCA 1 cut(s) 1999
MluNI TGGCCA 1 cut(s) 1999
MlyI GAGTC 2 cut(s) 317, 1293
MmeI TCCRAC 4 cut(s) 968, 1456, 1613, 2925
Mox20I TGGCCA 1 cut(s) 1999
Mph1103I ATGCAT 1 cut(s) 487
MroI TCCGGA 1 cut(s) 1876
MroNI GCCGGC 1 cut(s) 42
MroXI GAANNNNTTC 1 cut(s) 2208
MscI TGGCCA 1 cut(s) 1999
MseI TTAA 6 cut(s) 1499, 2045, 2139, 2298, 2481, 2679
MslI CAYNNNNRTG 4 cut(s) 760, 909, 1016, 2462
Msp20I TGGCCA 1 cut(s) 1999
MspA1I CMGCKG 1 cut(s) 1979
MspCI CTTAAG 1 cut(s) 2678
MspI CCGG 7 cut(s) 28, 43, 47, 458, 1022, 1877, 2951
MspR9I CCNGG 9 cut(s) 675, 876, 1023, 1149, 1302, 1485, 1553, 2519, 2816
MunI CAATTG 1 cut(s) 1426
MvaI CCWGG 8 cut(s) 675, 876, 1149, 1302, 1485, 1553, 2519, 2816
MvnI CGCG 2 cut(s) 120, 282
MwoI GCNNNNNNNGC 8 cut(s) 138, 491, 496, 961, 1520, 1529, 1623, 2496
NaeI GCCGGC 1 cut(s) 44
NciI CCSGG 1 cut(s) 1023
NgoMIV GCCGGC 1 cut(s) 42
NlaIV GGNNCC 6 cut(s) 633, 778, 1146, 2533, 2576, 2949
NmuCI GTSAC 5 cut(s) 437, 895, 1689, 2058, 2549
NruI TCGCGA 1 cut(s) 120
NsiI ATGCAT 1 cut(s) 487
NspI RCATGY 3 cut(s) 806, 908, 1518
PagI TCATGA 2 cut(s) 1641, 2731
PceI AGGCCT 1 cut(s) 499
PciI ACATGT 1 cut(s) 904
PciSI GCTCTTC 1 cut(s) 168
PcsI WCGNNNNNNNCGW 1 cut(s) 2889
PdiI GCCGGC 1 cut(s) 44
PdmI GAANNNNTTC 1 cut(s) 2208
PfeI GAWTC 5 cut(s) 318, 1214, 1547, 1964, 2171
PflMI CCANNNNNTGG 1 cut(s) 828
PfoI TCCNGGA 1 cut(s) 874
PinAI ACCGGT 1 cut(s) 457
PkrI GCNGC 3 cut(s) 255, 2103, 2672
PleI GAGTC 2 cut(s) 317, 1292
PpsI GAGTC 2 cut(s) 317, 1292
Ppu21I YACGTR 1 cut(s) 572
PscI ACATGT 1 cut(s) 904
PsiI TTATAA 3 cut(s) 1251, 1815, 2700
Psp124BI GAGCTC 3 cut(s) 87, 569, 1421
Psp6I CCWGG 8 cut(s) 673, 874, 1147, 1300, 1483, 1551, 2517, 2814
PspEI GGTNACC 1 cut(s) 2549
PspGI CCWGG 8 cut(s) 673, 874, 1147, 1300, 1483, 1551, 2517, 2814
PspN4I GGNNCC 6 cut(s) 633, 778, 1146, 2533, 2576, 2949
PspPI GGNCC 3 cut(s) 215, 776, 861
PsrI GAACNNNNNNTAC 2 cut(s) 2407, 2439
PsuI RGATCY 2 cut(s) 631, 2308
PvuII CAGCTG 1 cut(s) 1979
RruI TCGCGA 1 cut(s) 120
RsaI GTAC 8 cut(s) 227, 338, 699, 885, 903, 1318, 1824, 2378
RsaNI GTAC 8 cut(s) 226, 337, 698, 884, 902, 1317, 1823, 2377
RseI CAYNNNNRTG 4 cut(s) 760, 909, 1016, 2462
SacI GAGCTC 3 cut(s) 87, 569, 1421
SapI GCTCTTC 1 cut(s) 168
SaqAI TTAA 6 cut(s) 1499, 2045, 2139, 2298, 2481, 2679
SatI GCNGC 3 cut(s) 254, 2102, 2671
Sau96I GGNCC 3 cut(s) 215, 776, 861
ScaI AGTACT 1 cut(s) 1318
SchI GAGTC 2 cut(s) 317, 1293
ScrFI CCNGG 9 cut(s) 675, 876, 1023, 1149, 1302, 1485, 1553, 2519, 2816
SduI GDGCHC 5 cut(s) 87, 569, 1013, 1421, 2579
SexAI ACCWGGT 1 cut(s) 1147
SfaNI GCATC 3 cut(s) 472, 2009, 2732
SfcI CTRYAG 3 cut(s) 888, 1723, 2610
SfiI GGCCNNNNNGGCC 1 cut(s) 496
SinI GGWCC 2 cut(s) 215, 776
SmiMI CAYNNNNRTG 4 cut(s) 760, 909, 1016, 2462
SmlI CTYRAG 4 cut(s) 1715, 2284, 2678, 2846
SmoI CTYRAG 4 cut(s) 1715, 2284, 2678, 2846
SpeI ACTAGT 1 cut(s) 1328
SseBI AGGCCT 1 cut(s) 499
SsiI CCGC 3 cut(s) 40, 218, 635
SspI AATATT 1 cut(s) 934
SspMI CTAG 8 cut(s) 68, 965, 1044, 1269, 1329, 1733, 2319, 2580
SstI GAGCTC 3 cut(s) 87, 569, 1421
StuI AGGCCT 1 cut(s) 499
StyD4I CCNGG 9 cut(s) 673, 874, 1021, 1147, 1300, 1483, 1551, 2517, 2814
StyI CCWWGG 3 cut(s) 1412, 1732, 1826
TaiI ACGT 4 cut(s) 126, 574, 1691, 1776
TaqI TCGA 1 cut(s) 321
TatI WGTACW 5 cut(s) 225, 336, 901, 1316, 2376
TfiI GAWTC 5 cut(s) 318, 1214, 1547, 1964, 2171
Tru1I TTAA 6 cut(s) 1499, 2045, 2139, 2298, 2481, 2679
Tru9I TTAA 6 cut(s) 1499, 2045, 2139, 2298, 2481, 2679
TseFI GTSAC 5 cut(s) 437, 895, 1689, 2058, 2549
TseI GCWGC 3 cut(s) 253, 2101, 2670
Tsp45I GTSAC 5 cut(s) 437, 895, 1689, 2058, 2549
TspGWI ACGGA 1 cut(s) 2096
Van91I CCANNNNNTGG 1 cut(s) 828
Vha464I CTTAAG 1 cut(s) 2678
VneI GTGCAC 1 cut(s) 1009
VpaK11BI GGWCC 2 cut(s) 215, 776
XapI RAATTY 4 cut(s) 1108, 1124, 1560, 1662
XceI RCATGY 3 cut(s) 806, 908, 1518
XmaJI CCTAGG 1 cut(s) 1732
XmnI GAANNNNTTC 1 cut(s) 2208
XspI CTAG 8 cut(s) 68, 965, 1044, 1269, 1329, 1733, 2319, 2580
ZrmI AGTACT 1 cut(s) 1318
Zsp2I ATGCAT 1 cut(s) 487
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.