Rroxscaffold_3G00222160

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0000630
Physical Location & Seq
Forward (+)
5155668 .. 5163836
8169 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00222160.1

Sequence Viewer

Length: 3021 bp
ATGGAGATTTCTGAAGCCCCTCCCTTCCGGCCACGAGACCGCCGGCCGGACGCCCTCGGAAGCTTCCTAGTCCTCCCCGACGAGCTCATCTGTGCCGTCTTGGAGTTGCTCTCTCCTCGCGACGTTGCTCGCCTCTCATGCGTTAGCAGTGTGATGTATATATTTTGCAATGAAGAGCCACTCTGGTTGAGTTTATGCCTCAACACACTGAATGGTCCGCTCCAGTACAAAGGATCGTGGAAGAAGACGGTGCTGCATTTGGAGAATGTGTCGTATGAACGAGACGAGGCTTGCAGAAAACCACTGAGTTTTGATGGTTTTCCCTGGCTGAAGGCGTTTGTCATTGCCTATCTACTTGGTGCAGGATTCGACTCTTTGTTCTTGTACAGAAGATTATACAGGTGTCATACCACATTGGATGGTTTTTCTTTTGACAATGGGAATGTGGAAAGAAAGGACAAGATCACATTGGAAGAGTTTTCTTGTGATTATGATGGGAAGAAACCGGTTTTGCTCACTGGATTAGCTGATGCATGGCCTGCAAGGCGTACATGGACACTTGACCACTTATTGCAGAATTATGGAGATACTGCTTTCAAGATTTCTCAAAGGAGCTCACGTAAAATCTCAATGACATTCAAAGATTATGTTTCATATATGAAGGTTCAGCATGATGAGGATCCGCTTTATATCTTTGATCACAAGTTTGGAGAGGTTGAACCTGGCTTGTTGAAAGATTACAGTGTACCTTATCTATTTCAAGAGGACTTTTTTGATGTTTTGGACAAAGATAAGCGACCTCCATTTAGATGGCTCATTATTGGTCCCCAGAGGTCTGGTGCTTCTTGGCATGTTGATCCAGCTCTGACCAGTGCCTGGAATACACTTCTATGTGGTCGTAAAAGGTGGGCCTTGTATCCTCCAGGAAGAGTACCTGTAGGTGTCACAGTACATGTAAACGAAGAAGACGGTGATGTCAATATTGAAACTCCAACTTCGTTGCAGTGGTGGCTAGATTTTTATCCACTTCTTGCTGATGAAGACAAGCCCATAGAGTGCACCCAAATGCCGGGGGAGACGATTTTCGTTCCTAGTGGATGGTGGCACTGTGTCCTAAATCTTGAACCATCTATTGCTGTTACCCAGAACTTTGTAAATTCTAAGAACTTTGAATTTGTGTGCTTGGATATGGCGCCTGGTTATCGTCATAAGGGAGTTTGCCGTGCTGGATTACTTGCAGATGATGAAGGCGGTATTGAGGATTCCACAAATCATATTCTCTATGATAAAGATGATTATAATTCCTCAGATATGACTAGGAAAGTAAAAAGAGTCAGAACTCTCAAACCTGGAGAATATCCTAGAAGTGAAAGAACTAGTAATGGTGCTCAGGTTTTTTCTTATGATGTAAATTTCTTAGCCATGTATCTGGATGAAGAGAGAGATCATTACAACTCTCCTTGGAGCTCAGGCAATTGCATAGGGCAACGAGAAATGAGAGAATGGTTATTCAAGCTTTGGATTGGAAAACCAGGAATGAGAGACTTGATCTGGAAGGGAGCATGTCTTGCACTAAATGCTAGCAAATGGTCAGAATCCCTGGCTGAAATTTGTGCCTTCCACAAATTGCCTTCTCCAACAGATGATGAGAGGCTTCCAGTTGGCACGGGTAGCAATCCTGTTTATCTCATGAGCAACTGCGTAATCAAAATTTTTGTTGAGGAAGGACTGGAAACTTCACTTTATGGATTAGGTGCTGAGCTTGAGTTCTACAGTCTTCTAGGCAAAGTTAACTCCCCTCTGAAAAATCACGTACCTGACGTCTTGGCAAGTGGGATCATTTATCTTGAAAATGGAACGTATAAAATTGTACCTTGGGATGGCATCAGAGTTCCTGATGTGATTGCTAACTGCAATTTTATTCCGGAGAGGGTCAAGGGAGATGTCTCTCCTTTTGGTGTATGGAGGAAGAAACAATTTGAATACAGAAAAGCTGGGGTCTCAACAAACGAATCCATCAGTTCAGCTGAATATACAAGGATATGGCCGTATCTCATAACAAAACGATGCAAAGGAAAAATATATGCAGAGTTAAGAGATACTGTGTCACGGGAAGATGAACTGAACTTAGCTTCCTTCCTGGGACAGCAGCTCCGTAACCTCCATCTCTTGCCCCATCCACCTCTTAATATCTCAACTTTCTCAGATATTGAACAGGGATCAGATTTTCCATTCACCAACGGTAGTATGGAAGCTGTTCCCCATAAATCAAACATTCCTGCTGAATGGGATATATTCATTAGAACTCTATTCAAGAAGAAGAAGGATGTCTCAAGTCGCTTGATTAAATGGGGAGATCCAATTCCTAGCACACTCATAGAGAAAGTTGACAAGTACATCCCAGATGATTTTGCCAAGTTTCTGTACATGTTCAAGGATGAAAATGGTGTGAGCAAAGTTGGTAAAAGTTGTTCGTGGATACACTCTGATATCATGGATGACAACATTCACATGGAACTATGTGGTTTTAATTCTTGCTTCATTGGCAACGCCAAAGACACTTGCCTGGTAAATAATGGTTCCTTGAATGTTGATGGTGACCGTGCAGAGAGGAAAACATGGTGCCCTAGCCACATTCTTGATTTCAGTAATCTTTCTATAGGTGATCCCATCTATGATTTGATACCCTTATACTTGGATATATTCAGAGGTGATTGCTACCTCCTTAAGCAATTTCTAGATAGTTATAAACTTCCTTTAGTAAGACAAGCATCACCTCATGAGTATATGGAGGGTGGCGACAAGTTTGGACGACTTTCCTACCATGCCATGTGCTACTGTATTCTACATGAAGAGAATGTCCTGGGAGCAATTTTTAGTCTCTGGGACGAACTCAAGATGGCAAAATCATGGGAAGAAGTTGAACAGGTCGTATGGGGCGAGCTAAATAACTACAAAGGCTTTCCTGGATCATCCACACATCTCTCTTTGTTGGAGCTGAGAACTCCCTCAACATTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
APH Cupin_8 F-box F-box-like JmjC

Protein Analysis

1006

Amino Acids

115.07

Weight (kDa)

5.47

Isoelectric Point (pI)

42.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box-like PF12937 23 - 67 3.3e-07 F-box-like
Cupin_8 PF13621 165 - 387 1.8e-19 Cupin-like domain
JmjC PF02373 271 - 384 8.4e-15 JmjC domain, hydroxylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000630)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G78280
fragaria_vesca FvH4_5g36800 FvH4_5g36800 FvH4_5g36800 FvH4_5g36800 FvH4_5g36800 FvH4_5g36800 FvH4_5g36800 FvH4_5g36800 FvH4_5g36800 FvH4_5g36800 FvH4_5g36800 FvH4_5g36800 FvH4_5g36800 FvH4_5g36800 FvH4_5g36800 FvH4_5g36800 FvH4_5g36800 FvH4_5g36800
malus_domestica MD08G1186800.v1.1 MD15G1372700.v1.1
prunus_persica Prupe.1G521300_v2.0.a1 Prupe.1G521300_v2.0.a1
pyrus_communis pycom08g16060 pycom15g33370
rosa_chinensis RchiOBHm_Chr2g0139391 RchiOBHm_Chr7g0242931 RchiOBHm_Chr7g0242951 RchiOBHm_Chr7g0243001 RchiOBHm_Chr7g0243011 RchiOBHm_Chr7g0243141 RchiOBHm_Chr7g0243151 RchiOBHm_Chr7g0243351
rosa_laevigata RLG00000000853 RLG00000000857 RLG00000000858 RLG00000000863 RLG00000000867
rosa_multiflora Rmu_co8457323.1_g000001 Rmu_sc0000083.1_g000017 Rmu_sc0000679.1_g000021 Rmu_sc0001036.1_g000029 Rmu_sc0001036.1_g000032 Rmu_sc0001176.1_g000015 Rmu_sc0001461.1_g000002 Rmu_sc0001461.1_g000003 Rmu_sc0001461.1_g000056 Rmu_sc0001461.1_g000057 Rmu_sc0001461.1_g000098 Rmu_sc0001461.1_g000105 Rmu_sc0004165.1_g000084 Rmu_sc0006141.1_g000004 Rmu_sc0033806.1_g000001
rosa_roxburghii Rroxscaffold_3G00222160 Rroxscaffold_3G00222220 Rroxscaffold_4G00316290
rosa_rugosa Rorug07G0318400 Rorug07G0318400 Rorug07G0318600
rosa_samantha Rh4CG066500 Rh5DG444300 Rh7AG473700 Rh7AG473900 Rh7AG474000 Rh7AG474600 Rh7AG474800 Rh7BG444500 Rh7BG444700 Rh7CG490700 Rh7CG490800 Rh7CG491000 Rh7DG458900 Rh7DG459000 Rh7DG459300
rosa_wichuraiana Rw7G038970 Rw7G039050 Rw7G039420 Rw7G039480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1299, 2748
AasI GACNNNNNNGTC 1 cut(s) 974
AatII GACGTC 1 cut(s) 1824
AccB1I GGYRCC 2 cut(s) 1192, 2622
AccB7I CCANNNNNTGG 1 cut(s) 876
AccBSI CCGCTC 1 cut(s) 220
AccII CGCG 1 cut(s) 120
AccIII TCCGGA 1 cut(s) 1924
AciI CCGC 4 cut(s) 40, 218, 683, 1251
AclWI GGATC 9 cut(s) 241, 674, 687, 851, 1844, 2227, 2351, 2660, 2977
AcoI YGGCCR 3 cut(s) 29, 44, 2045
AcsI RAATTY 5 cut(s) 1156, 1172, 1411, 1608, 1710
AcuI CTGAAG 2 cut(s) 33, 350
AcyI GRCGYC 3 cut(s) 51, 1193, 1821
AfiI CCNNNNNNNGG 4 cut(s) 46, 876, 1069, 1880
AflII CTTAAG 1 cut(s) 2726
AflIII ACRYGT 2 cut(s) 952, 2427
AgeI ACCGGT 1 cut(s) 505
AhlI ACTAGT 1 cut(s) 1376
AloI GAACNNNNNNTCC 2 cut(s) 2117, 2149
Alw21I GWGCWC 5 cut(s) 87, 617, 1061, 1390, 1469
Alw26I GTCTC 8 cut(s) 30, 276, 1070, 1536, 1951, 2005, 2335, 2885
Alw44I GTGCAC 1 cut(s) 1057
AlwI GGATC 9 cut(s) 241, 674, 687, 851, 1844, 2227, 2351, 2660, 2977
AlwNI CAGNNNCTG 1 cut(s) 876
Aor13HI TCCGGA 1 cut(s) 1924
AoxI GGCC 5 cut(s) 29, 44, 536, 909, 2045
ApaLI GTGCAC 1 cut(s) 1057
ApeKI GCWGC 2 cut(s) 253, 2149
ApoI RAATTY 5 cut(s) 1156, 1172, 1411, 1608, 1710
AsiGI ACCGGT 1 cut(s) 505
Asp700I GAANNNNTTC 1 cut(s) 2256
AspLEI GCGC 1 cut(s) 1195
AspS9I GGNCC 3 cut(s) 215, 824, 909
AsuC2I CCSGG 1 cut(s) 1071
AsuHPI GGTGA 6 cut(s) 983, 2227, 2609, 2675, 2723, 2766
AsuNHI GCTAGC 1 cut(s) 1580
AvaII GGWCC 2 cut(s) 215, 824
BaeGI GKGCMC 2 cut(s) 1061, 2627
BamHI GGATCC 1 cut(s) 679
BanI GGYRCC 2 cut(s) 1192, 2622
BanII GRGCYC 3 cut(s) 87, 617, 1469
BauI CACGAG 1 cut(s) 33
BbsI GAAGAC 4 cut(s) 251, 972, 1047, 1769
Bbv12I GWGCWC 5 cut(s) 87, 617, 1061, 1390, 1469
BbvI GCAGC 2 cut(s) 240, 2161
BceAI ACGGC 3 cut(s) 80, 1206, 2032
BciVI GTATCC 2 cut(s) 927, 2472
BclI TGATCA 1 cut(s) 697
BcnI CCSGG 1 cut(s) 1071
BcoDI GTCTC 8 cut(s) 30, 276, 1070, 1536, 1951, 2005, 2335, 2885
BcuI ACTAGT 1 cut(s) 1376
BfmI CTRYAG 3 cut(s) 936, 1771, 2658
BfoI RGCGCY 1 cut(s) 1196
BfrI CTTAAG 1 cut(s) 2726
BfuI GTATCC 2 cut(s) 927, 2472
BglI GCCNNNNNGGC 1 cut(s) 544
BisI GCNGC 2 cut(s) 254, 2150
BlpI GCTNAGC 1 cut(s) 1758
BlsI GCNGC 2 cut(s) 255, 2151
Bme18I GGWCC 2 cut(s) 215, 824
BmgT120I GGNCC 3 cut(s) 215, 824, 909
BmiI GGNNCC 5 cut(s) 681, 826, 1194, 2581, 2624
BmsI GCATC 4 cut(s) 520, 1893, 2057, 2780
BmtI GCTAGC 1 cut(s) 1584
BpiI GAAGAC 4 cut(s) 251, 972, 1047, 1769
BplI GAGNNNNNCTC 2 cut(s) 95, 127
BpmI CTGGAG 3 cut(s) 206, 906, 1371
Bpu10I CCTNAGC 2 cut(s) 1389, 1468
Bpu1102I GCTNAGC 1 cut(s) 1758
BpuEI CTTGAG 3 cut(s) 1784, 2317, 2879
BpuMI CCSGG 1 cut(s) 1071
BsaAI YACGTR 2 cut(s) 620, 1813
BsaBI GATNNNNATC 2 cut(s) 678, 1020
BsaHI GRCGYC 3 cut(s) 51, 1193, 1821
BsaI GGTCTC 2 cut(s) 30, 2005
BsaJI CCNNGG 8 cut(s) 55, 323, 1070, 1460, 1599, 1874, 2140, 2863
BsaWI WCCGGW 2 cut(s) 505, 1924
BsaXI ACNNNNNCTCC 6 cut(s) 254, 284, 604, 634, 2136, 2166
Bsc4I CCNNNNNNNGG 4 cut(s) 46, 876, 1069, 1880
Bse118I RCCGGY 2 cut(s) 42, 505
Bse1I ACTGG 5 cut(s) 223, 523, 870, 1658, 1734
Bse3DI GCAATG 2 cut(s) 175, 342
Bse8I GATNNNNATC 2 cut(s) 678, 1020
BseAI TCCGGA 1 cut(s) 1924
BseDI CCNNGG 8 cut(s) 55, 323, 1070, 1460, 1599, 1874, 2140, 2863
BseJI GATNNNNATC 2 cut(s) 678, 1020
BseLI CCNNNNNNNGG 4 cut(s) 46, 876, 1069, 1880
BseMI GCAATG 2 cut(s) 175, 342
BseMII CTCAG 7 cut(s) 296, 1320, 1403, 1482, 1749, 2217, 2990
BseNI ACTGG 5 cut(s) 223, 523, 870, 1658, 1734
BseRI GAGGAG 1 cut(s) 105
BseSI GKGCMC 2 cut(s) 1061, 2627
BseX3I CGGCCG 1 cut(s) 44
BseXI GCAGC 2 cut(s) 240, 2161
BseYI CCCAGC 1 cut(s) 1994
BsgI GTGCAG 2 cut(s) 381, 2625
Bsh1236I CGCG 1 cut(s) 120
Bsh1285I CGRYCG 1 cut(s) 47
BshFI GGCC 5 cut(s) 31, 46, 538, 911, 2047
BshNI GGYRCC 2 cut(s) 1192, 2622
BshTI ACCGGT 1 cut(s) 505
BsiEI CGRYCG 1 cut(s) 47
BsiHKAI GWGCWC 5 cut(s) 87, 617, 1061, 1390, 1469
BsiSI CCGG 6 cut(s) 28, 43, 47, 506, 1070, 1925
BslFI GGGAC 3 cut(s) 810, 2157, 2900
BslI CCNNNNNNNGG 4 cut(s) 46, 876, 1069, 1880
BsmAI GTCTC 8 cut(s) 30, 276, 1070, 1536, 1951, 2005, 2335, 2885
BsmBI CGTCTC 2 cut(s) 276, 1070
BsmFI GGGAC 3 cut(s) 810, 2157, 2900
BsnI GGCC 5 cut(s) 31, 46, 538, 911, 2047
Bso31I GGTCTC 2 cut(s) 30, 2005
Bsp1286I GDGCHC 6 cut(s) 87, 617, 1061, 1390, 1469, 2627
Bsp13I TCCGGA 1 cut(s) 1924
Bsp1407I TGTACA 2 cut(s) 384, 2424
Bsp1720I GCTNAGC 1 cut(s) 1758
Bsp68I TCGCGA 1 cut(s) 120
BspACI CCGC 4 cut(s) 40, 218, 683, 1251
BspANI GGCC 5 cut(s) 31, 46, 538, 911, 2047
BspCNI CTCAG 7 cut(s) 297, 1319, 1402, 1481, 1750, 2216, 2991
BspEI TCCGGA 1 cut(s) 1924
BspFNI CGCG 1 cut(s) 120
BspHI TCATGA 2 cut(s) 1689, 2779
BspLI GGNNCC 5 cut(s) 681, 826, 1194, 2581, 2624
BspOI GCTAGC 1 cut(s) 1584
BspPI GGATC 9 cut(s) 241, 674, 687, 851, 1844, 2227, 2351, 2660, 2977
BspQI GCTCTTC 1 cut(s) 168
BspT107I GGYRCC 2 cut(s) 1192, 2622
BspTI CTTAAG 1 cut(s) 2726
BspTNI GGTCTC 2 cut(s) 30, 2005
BsrBI CCGCTC 1 cut(s) 220
BsrDI GCAATG 2 cut(s) 175, 342
BsrFI RCCGGY 2 cut(s) 42, 505
BsrGI TGTACA 2 cut(s) 384, 2424
BsrI ACTGG 5 cut(s) 223, 523, 870, 1658, 1734
BssAI RCCGGY 2 cut(s) 42, 505
BssECI CCNNGG 8 cut(s) 55, 323, 1070, 1460, 1599, 1874, 2140, 2863
BssNI GRCGYC 3 cut(s) 51, 1193, 1821
BssSI CACGAG 1 cut(s) 33
BssT1I CCWWGG 2 cut(s) 1460, 1874
Bst2BI CACGAG 1 cut(s) 33
Bst6I CTCTTC 5 cut(s) 168, 468, 922, 1431, 2847
BstACI GRCGYC 3 cut(s) 51, 1193, 1821
BstAFI CTTAAG 1 cut(s) 2726
BstAPI GCANNNNNTGC 3 cut(s) 539, 1568, 1577
BstAUI TGTACA 2 cut(s) 384, 2424
BstBAI YACGTR 2 cut(s) 620, 1813
BstC8I GCNNGC 6 cut(s) 44, 130, 292, 540, 1582, 2942
BstEII GGTNACC 1 cut(s) 2597
BstFNI CGCG 1 cut(s) 120
BstH2I RGCGCY 1 cut(s) 1196
BstHHI GCGC 1 cut(s) 1195
BstMAI GTCTC 8 cut(s) 30, 276, 1070, 1536, 1951, 2005, 2335, 2885
BstMCI CGRYCG 1 cut(s) 47
BstMWI GCNNNNNNNGC 8 cut(s) 138, 539, 544, 1009, 1568, 1577, 1671, 2544
BstNSI RCATGY 4 cut(s) 854, 956, 1566, 2431
BstPI GGTNACC 1 cut(s) 2597
BstSFI CTRYAG 3 cut(s) 936, 1771, 2658
BstSLI GKGCMC 2 cut(s) 1061, 2627
BstUI CGCG 1 cut(s) 120
BstV1I GCAGC 2 cut(s) 240, 2161
BstV2I GAAGAC 4 cut(s) 251, 972, 1047, 1769
BstX2I RGATCY 2 cut(s) 679, 2356
BstXI CCANNNNNNTGG 3 cut(s) 810, 836, 1429
BstYI RGATCY 2 cut(s) 679, 2356
BstZI CGGCCG 1 cut(s) 44
BsuI GTATCC 2 cut(s) 927, 2472
BsuRI GGCC 5 cut(s) 31, 46, 538, 911, 2047
BtsI GCAGTG 2 cut(s) 154, 1010
BtsIMutI CAGTG 8 cut(s) 154, 206, 302, 516, 748, 877, 1010, 1105
BtuMI TCGCGA 1 cut(s) 120
Cac8I GCNNGC 6 cut(s) 44, 130, 292, 540, 1582, 2942
CaiI CAGNNNCTG 1 cut(s) 876
CciI TCATGA 2 cut(s) 1689, 2779
CfoI GCGC 1 cut(s) 1195
Cfr10I RCCGGY 2 cut(s) 42, 505
Cfr13I GGNCC 3 cut(s) 215, 824, 909
CseI GACGC 1 cut(s) 59
CspAI ACCGGT 1 cut(s) 505
CspCI CAANNNNNGTGG 2 cut(s) 168, 203
DinI GGCGCC 1 cut(s) 1194
DrdI GACNNNNNNGTC 1 cut(s) 974
DseDI GACNNNNNNGTC 1 cut(s) 974
EaeI YGGCCR 3 cut(s) 29, 44, 2045
EagI CGGCCG 1 cut(s) 44
Eam1104I CTCTTC 5 cut(s) 168, 468, 922, 1431, 2847
EarI CTCTTC 5 cut(s) 168, 468, 922, 1431, 2847
Ecl136II GAGCTC 3 cut(s) 85, 615, 1467
EclXI CGGCCG 1 cut(s) 44
Eco130I CCWWGG 2 cut(s) 1460, 1874
Eco24I GRGCYC 3 cut(s) 87, 617, 1469
Eco31I GGTCTC 2 cut(s) 30, 2005
Eco32I GATATC 1 cut(s) 2491
Eco47I GGWCC 2 cut(s) 215, 824
Eco52I CGGCCG 1 cut(s) 44
Eco53kI GAGCTC 3 cut(s) 85, 615, 1467
Eco57I CTGAAG 2 cut(s) 33, 350
Eco91I GGTNACC 1 cut(s) 2597
EcoICRI GAGCTC 3 cut(s) 85, 615, 1467
EcoO65I GGTNACC 1 cut(s) 2597
EcoRV GATATC 1 cut(s) 2491
EcoT14I CCWWGG 2 cut(s) 1460, 1874
EcoT22I ATGCAT 1 cut(s) 535
EcoT38I GRGCYC 3 cut(s) 87, 617, 1469
EgeI GGCGCC 1 cut(s) 1194
EheI GGCGCC 1 cut(s) 1194
ErhI CCWWGG 2 cut(s) 1460, 1874
Esp3I CGTCTC 2 cut(s) 276, 1070
FaqI GGGAC 3 cut(s) 810, 2157, 2900
FbaI TGATCA 1 cut(s) 697
Fnu4HI GCNGC 2 cut(s) 254, 2150
FriOI GRGCYC 3 cut(s) 87, 617, 1469
Fsp4HI GCNGC 2 cut(s) 254, 2150
GlaI GCGC 1 cut(s) 1194
GluI GCNGC 2 cut(s) 254, 2150
GsaI CCCAGC 1 cut(s) 1998
GsuI CTGGAG 3 cut(s) 206, 906, 1371
HaeII RGCGCY 1 cut(s) 1196
HaeIII GGCC 5 cut(s) 31, 46, 538, 911, 2047
HapII CCGG 6 cut(s) 28, 43, 47, 506, 1070, 1925
HgaI GACGC 1 cut(s) 59
HhaI GCGC 1 cut(s) 1195
Hin1I GRCGYC 3 cut(s) 51, 1193, 1821
Hin6I GCGC 1 cut(s) 1193
HinP1I GCGC 1 cut(s) 1193
HincII GTYRAC 2 cut(s) 1792, 2389
HindII GTYRAC 2 cut(s) 1792, 2389
HindIII AAGCTT 2 cut(s) 61, 1514
HinfI GANTC 6 cut(s) 366, 371, 1262, 1332, 1595, 2012
HpaI GTTAAC 1 cut(s) 1792
HpaII CCGG 6 cut(s) 28, 43, 47, 506, 1070, 1925
HphI GGTGA 6 cut(s) 983, 2227, 2609, 2675, 2723, 2766
Hpy166II GTNNAC 5 cut(s) 746, 958, 1059, 1792, 2389
Hpy8I GTNNAC 5 cut(s) 746, 958, 1059, 1792, 2389
Hpy99I CGWCG 2 cut(s) 83, 125
HpyCH4IV ACGT 5 cut(s) 123, 619, 1812, 1821, 1859
HpyF10VI GCNNNNNNNGC 8 cut(s) 138, 539, 544, 1009, 1568, 1577, 1671, 2544
HpySE526I ACGT 5 cut(s) 123, 619, 1812, 1821, 1859
Hsp92I GRCGYC 3 cut(s) 51, 1193, 1821
HspAI GCGC 1 cut(s) 1193
KasI GGCGCC 1 cut(s) 1192
Kpn2I TCCGGA 1 cut(s) 1924
KroI GCCGGC 1 cut(s) 42
KroNI GCCGGC 1 cut(s) 44
Ksp22I TGATCA 1 cut(s) 697
KspAI GTTAAC 1 cut(s) 1792
LguI GCTCTTC 1 cut(s) 168
LmnI GCTCC 7 cut(s) 225, 612, 1464, 1559, 2157, 2867, 2995
Lsp1109I GCAGC 2 cut(s) 240, 2161
LweI GCATC 4 cut(s) 520, 1893, 2057, 2780
MaeII ACGT 5 cut(s) 123, 619, 1812, 1821, 1859
MaeIII GTNAC 5 cut(s) 943, 1138, 2106, 2156, 2597
MbiI CCGCTC 1 cut(s) 220
MfeI CAATTG 1 cut(s) 1474
MflI RGATCY 2 cut(s) 679, 2356
MhlI GDGCHC 6 cut(s) 87, 617, 1061, 1390, 1469, 2627
Mly113I GGCGCC 1 cut(s) 1193
MlyI GAGTC 2 cut(s) 365, 1341
MmeI TCCRAC 3 cut(s) 1016, 1661, 2973
Mph1103I ATGCAT 1 cut(s) 535
MroI TCCGGA 1 cut(s) 1924
MroNI GCCGGC 1 cut(s) 42
MroXI GAANNNNTTC 1 cut(s) 2256
MseI TTAA 6 cut(s) 1791, 2093, 2187, 2346, 2529, 2727
MslI CAYNNNNRTG 4 cut(s) 808, 889, 1064, 2510
MspA1I CMGCKG 1 cut(s) 2027
MspCI CTTAAG 1 cut(s) 2726
MspI CCGG 6 cut(s) 28, 43, 47, 506, 1070, 1925
MunI CAATTG 1 cut(s) 1474
MvnI CGCG 1 cut(s) 120
MwoI GCNNNNNNNGC 8 cut(s) 138, 539, 544, 1009, 1568, 1577, 1671, 2544
NaeI GCCGGC 1 cut(s) 44
NarI GGCGCC 1 cut(s) 1193
NciI CCSGG 1 cut(s) 1071
NgoMIV GCCGGC 1 cut(s) 42
NheI GCTAGC 1 cut(s) 1580
NlaIV GGNNCC 5 cut(s) 681, 826, 1194, 2581, 2624
NmuCI GTSAC 3 cut(s) 943, 2106, 2597
NruI TCGCGA 1 cut(s) 120
NsiI ATGCAT 1 cut(s) 535
NspI RCATGY 4 cut(s) 854, 956, 1566, 2431
PagI TCATGA 2 cut(s) 1689, 2779
PciI ACATGT 2 cut(s) 952, 2427
PciSI GCTCTTC 1 cut(s) 168
PcsI WCGNNNNNNNCGW 2 cut(s) 1818, 2937
PdiI GCCGGC 1 cut(s) 44
PdmI GAANNNNTTC 1 cut(s) 2256
PfeI GAWTC 4 cut(s) 366, 1262, 1595, 2012
PflMI CCANNNNNTGG 1 cut(s) 876
PfoI TCCNGGA 2 cut(s) 922, 2965
PinAI ACCGGT 1 cut(s) 505
PkrI GCNGC 2 cut(s) 255, 2151
PleI GAGTC 2 cut(s) 365, 1340
PluTI GGCGCC 1 cut(s) 1196
PpsI GAGTC 2 cut(s) 365, 1340
Ppu21I YACGTR 2 cut(s) 620, 1813
PscI ACATGT 2 cut(s) 952, 2427
PsiI TTATAA 2 cut(s) 1299, 2748
Psp124BI GAGCTC 3 cut(s) 87, 617, 1469
PspEI GGTNACC 1 cut(s) 2597
PspFI CCCAGC 1 cut(s) 1994
PspN4I GGNNCC 5 cut(s) 681, 826, 1194, 2581, 2624
PspPI GGNCC 3 cut(s) 215, 824, 909
PsrI GAACNNNNNNTAC 2 cut(s) 2455, 2487
PstNI CAGNNNCTG 1 cut(s) 876
PsuI RGATCY 2 cut(s) 679, 2356
PvuII CAGCTG 1 cut(s) 2027
RruI TCGCGA 1 cut(s) 120
RseI CAYNNNNRTG 4 cut(s) 808, 889, 1064, 2510
SacI GAGCTC 3 cut(s) 87, 617, 1469
SapI GCTCTTC 1 cut(s) 168
SaqAI TTAA 6 cut(s) 1791, 2093, 2187, 2346, 2529, 2727
SatI GCNGC 2 cut(s) 254, 2150
Sau96I GGNCC 3 cut(s) 215, 824, 909
SchI GAGTC 2 cut(s) 365, 1341
SduI GDGCHC 6 cut(s) 87, 617, 1061, 1390, 1469, 2627
SfaNI GCATC 4 cut(s) 520, 1893, 2057, 2780
SfcI CTRYAG 3 cut(s) 936, 1771, 2658
SfoI GGCGCC 1 cut(s) 1194
SinI GGWCC 2 cut(s) 215, 824
SmiMI CAYNNNNRTG 4 cut(s) 808, 889, 1064, 2510
SmlI CTYRAG 4 cut(s) 1763, 2332, 2726, 2894
SmoI CTYRAG 4 cut(s) 1763, 2332, 2726, 2894
SpeI ACTAGT 1 cut(s) 1376
SsiI CCGC 4 cut(s) 40, 218, 683, 1251
SspDI GGCGCC 1 cut(s) 1192
SspI AATATT 1 cut(s) 982
SstI GAGCTC 3 cut(s) 87, 617, 1469
StyI CCWWGG 2 cut(s) 1460, 1874
TaiI ACGT 5 cut(s) 126, 622, 1815, 1824, 1862
TaqI TCGA 1 cut(s) 369
TatI WGTACW 5 cut(s) 225, 384, 949, 2394, 2424
TfiI GAWTC 4 cut(s) 366, 1262, 1595, 2012
Tru1I TTAA 6 cut(s) 1791, 2093, 2187, 2346, 2529, 2727
Tru9I TTAA 6 cut(s) 1791, 2093, 2187, 2346, 2529, 2727
TscAI CASTG 8 cut(s) 154, 213, 309, 523, 748, 877, 1010, 1112
TseFI GTSAC 3 cut(s) 943, 2106, 2597
TseI GCWGC 2 cut(s) 253, 2149
Tsp45I GTSAC 3 cut(s) 943, 2106, 2597
TspGWI ACGGA 1 cut(s) 2144
TspRI CASTG 8 cut(s) 154, 213, 309, 523, 748, 877, 1010, 1112
Van91I CCANNNNNTGG 1 cut(s) 876
Vha464I CTTAAG 1 cut(s) 2726
VneI GTGCAC 1 cut(s) 1057
VpaK11BI GGWCC 2 cut(s) 215, 824
XapI RAATTY 5 cut(s) 1156, 1172, 1411, 1608, 1710
XbaI TCTAGA 1 cut(s) 2737
XceI RCATGY 4 cut(s) 854, 956, 1566, 2431
XcmI CCANNNNNNNNNTGG 1 cut(s) 2245
XmnI GAANNNNTTC 1 cut(s) 2256
ZraI GACGTC 1 cut(s) 1822
Zsp2I ATGCAT 1 cut(s) 535
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.