Rh7AG474600

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0000630
Physical Location & Seq
Reverse (-)
65907746 .. 65915201
7456 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG474600.1

Sequence Viewer

Length: 2811 bp
ATGTCGCACGTGGCAATCGCCGATTCCGTCAAGTCTCCAAAACCACAGAGCCCGCCTTTCTTAACCGCCGTTTGCCACAGTCAGTATTCCTCGTCTCCCTTTCACTCAAACTCCCGCAGCTTCTCTCTATCAACTTCAATCTTCCGATGGAGGTTTCGGAAGCCCCTCCCTTCCGGCCAAGAGATCGCCGCCCGGACGCCCTCGGAAACTTCCCTAGTCCTCTCCAACAAGCTCATCTGTACCCTCTTGGAATCGTTCTCTCCTCGCGACGTTGCTCGCCTCTCCTGCTTTAGCAGTGTGATGTATATACTTGGCAATGATGAGCCACTCTGGTTGGGTTTATGCCTCAACACATCGTGGAAGAAGACGGTTTTGCTCACTGGATTAGCTGATGCATGGCCTGCAAGGCATACATGGACACTTGACCACTTATTGCAGAATTATGGAGATACAGCTTTCAAGATTTCTCAAAGGACCTCACGTAAAATCTCAATGACATTCAAGGATTATGTTTCATATATGAAAGTTCAGCATGATGAGGATCCGCTTTATATCTTTGATCACAAGTTTGGAGAGGTCGAACCTGGCTTGTTGAAAGATTACAGTGTACCTTATCTATTTCAAGAGGACTTTTTTGATGTTTTGGACAAAGATAAGCGACCTCCATTTAGATGGCTCATTATTGGTCCCCAGAGGTGGGCCTTGTATCCTCCAGGAAGAGTACCTATAGGTGTCACAGTACATGTAAACGAAGAAGACGGTGATGTCAATATTGAAACTCCAACTTCGTTGCAGTGGTGGCTAGATTTTTATCCACTTCTTGCTGATGAAGACAAGCCCATAGAGTGCACCCAAATGCCGGGGGAGACAATTTTCGTTCCTAGTGGATGGTGGCACTGTGTCCTAAATCTTGAACCATCTATTGCTGTTACCCAGAACTTTGTAAATTCTAAGAACTTTGAATTTGTGTGCTTGGATATGGCACCTGGTTATCGTCATAAGGGAGTTTGCCGTGCTGGATTACTTGCAGATGATGAAGGCAGTATTGAGGATTCCACAAATCATATACTCTATGATAAAGATGATTATAATTCCTCAGATATGACTAGGAAAGTAAAAAGAGTCAGAACTCTCAAACCTGGAGAATATCCAAGTAGTGAAAGAACTAGTAATGGCGCTCAGGCTTTTTCTTATGATGTAAATTTCCTAGCCATGTATCTGGATGAAGAGAGAGATCATTACAACTCTCCTTGGAGCTCAGGCAATTGCATAGGGCAACGAGAAATGAGAGAATGGTTATTCAAGCTTTGGGTTGGAAAACCAGGAATGAGAGACTTAATCTGGAAGGGAGCATGTCTTGCACTAAATGCGAGCAAATGGTCAGAATCCCTGGCAGAAATTTGTGCCTTCCACAAATTGCCTTCTCCAACAGATGATGAGAGGCTTCCTGTTGGCACGGGTAGCAATCCTGTTTATCTCATGAGCAACTGTGTAATCAAAATTTTTGTTGACGGAGGACTGGAAACGTCACTTTATGGATTAGGTGCTGAGCTTGAGTTCTACAGTCTCCTAGGCAAAGTCAAATCCCCTCTGAAAAATCACATACCTGACATTTTGGCAAGTGGGATCATTTATCTTGAAAATGGAACTTATAAAATTGTACCTTGGGATGGCAACGGAGTTCCTGATGTGATTGCTAAGTGCAATTTTATTCCGGAGAAGGTCAAAGGAGATGTTTCTCCTTTTGGTGTATGGAGGAAGAAACAATTTGAATACAGAAAAGCTGGGGTCTCAACAAACGAATCCATCAGTTCAGTTGAATATACAAGGATATGGCCATATCTCATAACAAAACGATGCAGAGGAAAAATATATGCAGAGTTAAGAGATACTGTGTCACGGGAAGATGAATTGAACTTAGCTTCCTTCCTGGGAGAGCAGCTCTGTAACCTCCATCTCTTGCCCCATCCACCTCTTAATATCTCAACTTTCTCAGATATTGAACAGGAATCAGATTTTCCCTTCACCCGCGGTAGTATAGAAGCTGTTCCCAATAAATCAAACATTCCTGCTGAATGGGATATATTCATTAGAACTCTATCCAAGAAGAAGAAGGATGTCTCAAGTCGCTTGGTTAAATGGGGAGATCCAATTCCTAGTACACTCATAGAGAAAGTTGACAAATACATCCCAGATGATTTTGCCAAGTTTCTGTACATATTCAAGGATGAAAATGGTGTAAGCAATGTTGGTAAAAGTTGTTCGTGGATACACTCTGATATCATGGATGACAACATTCACATGGAACTATGTGGTTTTAATTCTTGTGTCATTGGCAATGCCAAAAACACTTGCCTGGTAAAGAATGGTTCCTTGAATGTTGATGGTGACCGTGCAGAGAGGAGAACATGGTGCCCTAGCCACATTCTTGATTTCAGTAATCTTTCTATAGGTGATCCCATCTATGATTTGATACCCTTATACTTGGATATATTCAGAGGAGATTGCTGCCTGCTTAAGGGATTTTTAGATAGTTATAAACTTCCTTTAGTGAGACAAGCATCACCTCATGAGTATATTGAGGGTGGCGACAAGTTTGGACGACTTTCATACCAGGCCATGTGCTACTGTATTCTACATGAAGAGAATGTCCTGGGAGCAATTTTTAGTCTCTGGGACGAACTCAAGATGGCAAAATCATGGGAAGAAGTTGAACAGGTCGTATGGGGCGAGCTAAACAACTACAAAGGCTTTCCTGGATCATCCACATCTCTCTCTTTGTTGGAGCCGAAAACTCCCTCAACATTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
APH Cupin_8 F-box F-box-like JmjC

Protein Analysis

936

Amino Acids

106.2

Weight (kDa)

5.61

Isoelectric Point (pI)

46.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cupin_8 PF13621 226 - 317 1.9e-08 Cupin-like domain
JmjC PF02373 276 - 314 4.4e-08 JmjC domain, hydroxylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000630)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G78280
fragaria_vesca FvH4_5g36800 FvH4_5g36800 FvH4_5g36800 FvH4_5g36800 FvH4_5g36800 FvH4_5g36800 FvH4_5g36800 FvH4_5g36800 FvH4_5g36800 FvH4_5g36800 FvH4_5g36800 FvH4_5g36800 FvH4_5g36800 FvH4_5g36800 FvH4_5g36800 FvH4_5g36800 FvH4_5g36800 FvH4_5g36800
malus_domestica MD08G1186800.v1.1 MD15G1372700.v1.1
prunus_persica Prupe.1G521300_v2.0.a1 Prupe.1G521300_v2.0.a1
pyrus_communis pycom08g16060 pycom15g33370
rosa_chinensis RchiOBHm_Chr2g0139391 RchiOBHm_Chr7g0242931 RchiOBHm_Chr7g0242951 RchiOBHm_Chr7g0243001 RchiOBHm_Chr7g0243011 RchiOBHm_Chr7g0243141 RchiOBHm_Chr7g0243151 RchiOBHm_Chr7g0243351
rosa_laevigata RLG00000000853 RLG00000000857 RLG00000000858 RLG00000000863 RLG00000000867
rosa_multiflora Rmu_co8457323.1_g000001 Rmu_sc0000083.1_g000017 Rmu_sc0000679.1_g000021 Rmu_sc0001036.1_g000029 Rmu_sc0001036.1_g000032 Rmu_sc0001176.1_g000015 Rmu_sc0001461.1_g000002 Rmu_sc0001461.1_g000003 Rmu_sc0001461.1_g000056 Rmu_sc0001461.1_g000057 Rmu_sc0001461.1_g000098 Rmu_sc0001461.1_g000105 Rmu_sc0004165.1_g000084 Rmu_sc0006141.1_g000004 Rmu_sc0033806.1_g000001
rosa_roxburghii Rroxscaffold_3G00222160 Rroxscaffold_3G00222220 Rroxscaffold_4G00316290
rosa_rugosa Rorug07G0318400 Rorug07G0318400 Rorug07G0318600
rosa_samantha Rh4CG066500 Rh5DG444300 Rh7AG473700 Rh7AG473900 Rh7AG474000 Rh7AG474600 Rh7AG474800 Rh7BG444500 Rh7BG444700 Rh7CG490700 Rh7CG490800 Rh7CG491000 Rh7DG458900 Rh7DG459000 Rh7DG459300
rosa_wichuraiana Rw7G038970 Rw7G039050 Rw7G039420 Rw7G039480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 4 cut(s) 1089, 1653, 2538, 2809
AasI GACNNNNNNGTC 1 cut(s) 764
AccB1I GGYRCC 2 cut(s) 982, 2412
AccII CGCG 2 cut(s) 267, 2031
AccIII TCCGGA 1 cut(s) 1714
AciI CCGC 7 cut(s) 53, 66, 115, 189, 545, 2029, 2031
AclWI GGATC 6 cut(s) 536, 549, 1634, 2141, 2450, 2767
AcoI YGGCCR 2 cut(s) 175, 1835
AcsI RAATTY 5 cut(s) 946, 962, 1201, 1398, 1500
AcvI CACGTG 1 cut(s) 10
AcyI GRCGYC 1 cut(s) 197
AdeI CACNNNGTG 1 cut(s) 357
AfaI GTAC 7 cut(s) 241, 609, 723, 741, 1662, 2161, 2216
AfiI CCNNNNNNNGG 5 cut(s) 696, 697, 859, 1670, 2518
AflII CTTAAG 1 cut(s) 2516
AflIII ACRYGT 1 cut(s) 742
AhlI ACTAGT 1 cut(s) 1166
AloI GAACNNNNNNTCC 2 cut(s) 1907, 1939
Alw21I GWGCWC 2 cut(s) 851, 1259
Alw26I GTCTC 9 cut(s) 39, 99, 860, 1326, 1571, 1795, 2125, 2548, 2675
Alw44I GTGCAC 1 cut(s) 847
AlwI GGATC 6 cut(s) 536, 549, 1634, 2141, 2450, 2767
Aor13HI TCCGGA 1 cut(s) 1714
AoxI GGCC 5 cut(s) 175, 398, 699, 1835, 2616
ApaLI GTGCAC 1 cut(s) 847
ApeKI GCWGC 3 cut(s) 117, 1939, 2508
ApoI RAATTY 5 cut(s) 946, 962, 1201, 1398, 1500
Asp700I GAANNNNTTC 1 cut(s) 2046
AspA2I CCTAGG 1 cut(s) 1570
AspLEI GCGC 1 cut(s) 1178
AspS9I GGNCC 3 cut(s) 474, 686, 699
AsuC2I CCSGG 2 cut(s) 193, 861
AsuHPI GGTGA 5 cut(s) 773, 2017, 2399, 2465, 2556
AvaII GGWCC 2 cut(s) 474, 686
AvrII CCTAGG 1 cut(s) 1570
BaeGI GKGCMC 2 cut(s) 851, 2417
BalI TGGCCA 1 cut(s) 1837
BamHI GGATCC 1 cut(s) 541
BanI GGYRCC 2 cut(s) 982, 2412
BanII GRGCYC 2 cut(s) 53, 1259
BbrPI CACGTG 1 cut(s) 10
BbsI GAAGAC 3 cut(s) 371, 762, 837
Bbv12I GWGCWC 2 cut(s) 851, 1259
BbvI GCAGC 3 cut(s) 129, 1951, 2495
BceAI ACGGC 2 cut(s) 53, 996
BciVI GTATCC 2 cut(s) 717, 2262
BclI TGATCA 1 cut(s) 559
BcnI CCSGG 2 cut(s) 193, 861
BcoDI GTCTC 9 cut(s) 39, 99, 860, 1326, 1571, 1795, 2125, 2548, 2675
BcuI ACTAGT 1 cut(s) 1166
BfaI CTAG 9 cut(s) 215, 803, 882, 1107, 1167, 1208, 1571, 2157, 2418
BfmI CTRYAG 3 cut(s) 726, 1561, 2448
BfoI RGCGCY 1 cut(s) 1179
BfrI CTTAAG 1 cut(s) 2516
BfuI GTATCC 2 cut(s) 717, 2262
BglI GCCNNNNNGGC 1 cut(s) 406
BisI GCNGC 4 cut(s) 118, 189, 1940, 2509
BlnI CCTAGG 1 cut(s) 1570
BlpI GCTNAGC 1 cut(s) 1548
BlsI GCNGC 4 cut(s) 119, 190, 1941, 2510
Bme18I GGWCC 2 cut(s) 474, 686
BmgT120I GGNCC 3 cut(s) 474, 686, 699
BmiI GGNNCC 6 cut(s) 543, 688, 984, 2371, 2414, 2787
BmsI GCATC 3 cut(s) 382, 1847, 2570
BpiI GAAGAC 3 cut(s) 371, 762, 837
BplI GAGNNNNNCTC 2 cut(s) 1926, 1958
BpmI CTGGAG 2 cut(s) 696, 1161
Bpu10I CCTNAGC 2 cut(s) 1179, 1258
Bpu1102I GCTNAGC 1 cut(s) 1548
BpuEI CTTGAG 3 cut(s) 1574, 2107, 2669
BpuMI CCSGG 2 cut(s) 193, 861
BsaAI YACGTR 2 cut(s) 10, 482
BsaBI GATNNNNATC 2 cut(s) 540, 810
BsaHI GRCGYC 1 cut(s) 197
BsaI GGTCTC 1 cut(s) 1795
BsaJI CCNNGG 9 cut(s) 201, 860, 1250, 1389, 1570, 1664, 1930, 2029, 2653
BsaWI WCCGGW 1 cut(s) 1714
BsaXI ACNNNNNCTCC 2 cut(s) 95, 125
Bsc4I CCNNNNNNNGG 5 cut(s) 696, 697, 859, 1670, 2518
Bse1I ACTGG 2 cut(s) 385, 1524
Bse3DI GCAATG 3 cut(s) 322, 2251, 2344
Bse8I GATNNNNATC 2 cut(s) 540, 810
BseAI TCCGGA 1 cut(s) 1714
BseDI CCNNGG 9 cut(s) 201, 860, 1250, 1389, 1570, 1664, 1930, 2029, 2653
BseGI GGATG 9 cut(s) 893, 1228, 1675, 1966, 2122, 2187, 2233, 2293, 2762
BseJI GATNNNNATC 2 cut(s) 540, 810
BseLI CCNNNNNNNGG 5 cut(s) 696, 697, 859, 1670, 2518
BseMI GCAATG 3 cut(s) 322, 2251, 2344
BseMII CTCAG 5 cut(s) 1110, 1193, 1272, 1539, 2007
BseNI ACTGG 2 cut(s) 385, 1524
BseRI GAGGAG 3 cut(s) 252, 2416, 2514
BseSI GKGCMC 2 cut(s) 851, 2417
BseXI GCAGC 3 cut(s) 129, 1951, 2495
BseYI CCCAGC 1 cut(s) 1784
BsgI GTGCAG 1 cut(s) 2415
Bsh1236I CGCG 2 cut(s) 267, 2031
BshFI GGCC 5 cut(s) 177, 400, 701, 1837, 2618
BshNI GGYRCC 2 cut(s) 982, 2412
BsiHKAI GWGCWC 2 cut(s) 851, 1259
BsiSI CCGG 4 cut(s) 174, 193, 860, 1715
BslFI GGGAC 2 cut(s) 672, 2690
BslI CCNNNNNNNGG 5 cut(s) 696, 697, 859, 1670, 2518
BsmAI GTCTC 9 cut(s) 39, 99, 860, 1326, 1571, 1795, 2125, 2548, 2675
BsmBI CGTCTC 1 cut(s) 99
BsmFI GGGAC 2 cut(s) 672, 2690
BsnI GGCC 5 cut(s) 177, 400, 701, 1837, 2618
Bso31I GGTCTC 1 cut(s) 1795
Bsp1286I GDGCHC 4 cut(s) 53, 851, 1259, 2417
Bsp13I TCCGGA 1 cut(s) 1714
Bsp1407I TGTACA 1 cut(s) 2214
Bsp143I GATC 8 cut(s) 183, 541, 559, 1234, 1626, 2146, 2455, 2759
Bsp1720I GCTNAGC 1 cut(s) 1548
Bsp68I TCGCGA 1 cut(s) 267
BspACI CCGC 7 cut(s) 53, 66, 115, 189, 545, 2029, 2031
BspANI GGCC 5 cut(s) 177, 400, 701, 1837, 2618
BspCNI CTCAG 5 cut(s) 1109, 1192, 1271, 1540, 2006
BspEI TCCGGA 1 cut(s) 1714
BspFNI CGCG 2 cut(s) 267, 2031
BspHI TCATGA 2 cut(s) 1479, 2569
BspLI GGNNCC 6 cut(s) 543, 688, 984, 2371, 2414, 2787
BspPI GGATC 6 cut(s) 536, 549, 1634, 2141, 2450, 2767
BspT107I GGYRCC 2 cut(s) 982, 2412
BspTI CTTAAG 1 cut(s) 2516
BspTNI GGTCTC 1 cut(s) 1795
BsrDI GCAATG 3 cut(s) 322, 2251, 2344
BsrGI TGTACA 1 cut(s) 2214
BsrI ACTGG 2 cut(s) 385, 1524
BssECI CCNNGG 9 cut(s) 201, 860, 1250, 1389, 1570, 1664, 1930, 2029, 2653
BssMI GATC 8 cut(s) 183, 541, 559, 1234, 1626, 2146, 2455, 2759
BssNI GRCGYC 1 cut(s) 197
BssT1I CCWWGG 3 cut(s) 1250, 1570, 1664
Bst6I CTCTTC 3 cut(s) 712, 1221, 2637
BstACI GRCGYC 1 cut(s) 197
BstAFI CTTAAG 1 cut(s) 2516
BstAPI GCANNNNNTGC 3 cut(s) 401, 1358, 1367
BstAUI TGTACA 1 cut(s) 2214
BstBAI YACGTR 2 cut(s) 10, 482
BstC8I GCNNGC 6 cut(s) 53, 277, 402, 1372, 2513, 2732
BstDEI CTNAG 8 cut(s) 951, 1096, 1179, 1258, 1548, 1698, 1918, 1993
BstDSI CCRYGG 1 cut(s) 2029
BstEII GGTNACC 1 cut(s) 2387
BstENI CCTNNNNNAGG 1 cut(s) 2516
BstF5I GGATG 9 cut(s) 893, 1228, 1675, 1966, 2122, 2187, 2233, 2293, 2762
BstFNI CGCG 2 cut(s) 267, 2031
BstH2I RGCGCY 1 cut(s) 1179
BstHHI GCGC 1 cut(s) 1178
BstKTI GATC 8 cut(s) 186, 544, 562, 1237, 1629, 2149, 2458, 2762
BstMAI GTCTC 9 cut(s) 39, 99, 860, 1326, 1571, 1795, 2125, 2548, 2675
BstMBI GATC 8 cut(s) 183, 541, 559, 1234, 1626, 2146, 2455, 2759
BstMWI GCNNNNNNNGC 7 cut(s) 285, 401, 406, 799, 1358, 1367, 1461
BstNSI RCATGY 2 cut(s) 746, 1356
BstPI GGTNACC 1 cut(s) 2387
BstSFI CTRYAG 3 cut(s) 726, 1561, 2448
BstSLI GKGCMC 2 cut(s) 851, 2417
BstUI CGCG 2 cut(s) 267, 2031
BstV1I GCAGC 3 cut(s) 129, 1951, 2495
BstV2I GAAGAC 3 cut(s) 371, 762, 837
BstX2I RGATCY 2 cut(s) 541, 2146
BstXI CCANNNNNNTGG 2 cut(s) 672, 1219
BstYI RGATCY 2 cut(s) 541, 2146
BsuI GTATCC 2 cut(s) 717, 2262
BsuRI GGCC 5 cut(s) 177, 400, 701, 1837, 2618
BtgI CCRYGG 1 cut(s) 2029
BtsCI GGATG 9 cut(s) 893, 1228, 1675, 1966, 2122, 2187, 2233, 2293, 2762
BtsI GCAGTG 2 cut(s) 301, 800
BtsIMutI CAGTG 5 cut(s) 301, 378, 610, 800, 895
BtuMI TCGCGA 1 cut(s) 267
Cac8I GCNNGC 6 cut(s) 53, 277, 402, 1372, 2513, 2732
CciI TCATGA 2 cut(s) 1479, 2569
CfoI GCGC 1 cut(s) 1178
Cfr13I GGNCC 3 cut(s) 474, 686, 699
Cfr42I CCGCGG 1 cut(s) 2032
CseI GACGC 1 cut(s) 205
CsiI ACCWGGT 1 cut(s) 985
Csp6I GTAC 7 cut(s) 240, 608, 722, 740, 1661, 2160, 2215
CspCI CAANNNNNGTGG 2 cut(s) 315, 350
CviQI GTAC 7 cut(s) 240, 608, 722, 740, 1661, 2160, 2215
DdeI CTNAG 8 cut(s) 951, 1096, 1179, 1258, 1548, 1698, 1918, 1993
DpnI GATC 8 cut(s) 185, 543, 561, 1236, 1628, 2148, 2457, 2761
DpnII GATC 8 cut(s) 183, 541, 559, 1234, 1626, 2146, 2455, 2759
DraIII CACNNNGTG 1 cut(s) 357
DrdI GACNNNNNNGTC 1 cut(s) 764
DseDI GACNNNNNNGTC 1 cut(s) 764
EaeI YGGCCR 2 cut(s) 175, 1835
Eam1104I CTCTTC 3 cut(s) 712, 1221, 2637
EarI CTCTTC 3 cut(s) 712, 1221, 2637
Ecl136II GAGCTC 1 cut(s) 1257
Eco130I CCWWGG 3 cut(s) 1250, 1570, 1664
Eco24I GRGCYC 2 cut(s) 53, 1259
Eco31I GGTCTC 1 cut(s) 1795
Eco32I GATATC 1 cut(s) 2281
Eco47I GGWCC 2 cut(s) 474, 686
Eco53kI GAGCTC 1 cut(s) 1257
Eco72I CACGTG 1 cut(s) 10
Eco91I GGTNACC 1 cut(s) 2387
EcoICRI GAGCTC 1 cut(s) 1257
EcoNI CCTNNNNNAGG 1 cut(s) 2516
EcoO109I RGGNCCY 1 cut(s) 474
EcoO65I GGTNACC 1 cut(s) 2387
EcoRV GATATC 1 cut(s) 2281
EcoT14I CCWWGG 3 cut(s) 1250, 1570, 1664
EcoT22I ATGCAT 1 cut(s) 397
EcoT38I GRGCYC 2 cut(s) 53, 1259
ErhI CCWWGG 3 cut(s) 1250, 1570, 1664
Esp3I CGTCTC 1 cut(s) 99
FaqI GGGAC 2 cut(s) 672, 2690
FauI CCCGC 3 cut(s) 60, 122, 2036
FbaI TGATCA 1 cut(s) 559
Fnu4HI GCNGC 4 cut(s) 118, 189, 1940, 2509
FokI GGATG 9 cut(s) 900, 1235, 1682, 1953, 2129, 2174, 2240, 2300, 2749
FriOI GRGCYC 2 cut(s) 53, 1259
Fsp4HI GCNGC 4 cut(s) 118, 189, 1940, 2509
FspBI CTAG 9 cut(s) 215, 803, 882, 1107, 1167, 1208, 1571, 2157, 2418
GlaI GCGC 1 cut(s) 1177
GluI GCNGC 4 cut(s) 118, 189, 1940, 2509
GsaI CCCAGC 1 cut(s) 1788
GsuI CTGGAG 2 cut(s) 696, 1161
HaeII RGCGCY 1 cut(s) 1179
HaeIII GGCC 5 cut(s) 177, 400, 701, 1837, 2618
HapII CCGG 4 cut(s) 174, 193, 860, 1715
HgaI GACGC 1 cut(s) 205
HhaI GCGC 1 cut(s) 1178
Hin1I GRCGYC 1 cut(s) 197
Hin6I GCGC 1 cut(s) 1176
HinP1I GCGC 1 cut(s) 1176
HincII GTYRAC 2 cut(s) 1510, 2179
HindII GTYRAC 2 cut(s) 1510, 2179
HindIII AAGCTT 1 cut(s) 1304
HinfI GANTC 7 cut(s) 23, 251, 1052, 1122, 1385, 1802, 2009
HpaII CCGG 4 cut(s) 174, 193, 860, 1715
HphI GGTGA 5 cut(s) 773, 2017, 2399, 2465, 2556
Hpy166II GTNNAC 6 cut(s) 608, 748, 849, 1510, 2162, 2179
Hpy8I GTNNAC 6 cut(s) 608, 748, 849, 1510, 2162, 2179
Hpy99I CGWCG 1 cut(s) 272
HpyAV CCTTC 9 cut(s) 180, 1031, 1339, 1417, 1431, 1714, 1936, 2032, 2107
HpyCH4IV ACGT 4 cut(s) 9, 270, 481, 1526
HpyF10VI GCNNNNNNNGC 7 cut(s) 285, 401, 406, 799, 1358, 1367, 1461
HpyF3I CTNAG 8 cut(s) 951, 1096, 1179, 1258, 1548, 1698, 1918, 1993
HpySE526I ACGT 4 cut(s) 9, 270, 481, 1526
Hsp92I GRCGYC 1 cut(s) 197
HspAI GCGC 1 cut(s) 1176
Kpn2I TCCGGA 1 cut(s) 1714
Ksp22I TGATCA 1 cut(s) 559
KspI CCGCGG 1 cut(s) 2032
Kzo9I GATC 8 cut(s) 183, 541, 559, 1234, 1626, 2146, 2455, 2759
LmnI GCTCC 4 cut(s) 1254, 1349, 2657, 2785
Lsp1109I GCAGC 3 cut(s) 129, 1951, 2495
LweI GCATC 3 cut(s) 382, 1847, 2570
MabI ACCWGGT 1 cut(s) 985
MaeI CTAG 9 cut(s) 215, 803, 882, 1107, 1167, 1208, 1571, 2157, 2418
MaeII ACGT 4 cut(s) 9, 270, 481, 1526
MaeIII GTNAC 6 cut(s) 733, 928, 1527, 1896, 1946, 2387
MalI GATC 8 cut(s) 185, 543, 561, 1236, 1628, 2148, 2457, 2761
MboI GATC 8 cut(s) 183, 541, 559, 1234, 1626, 2146, 2455, 2759
MfeI CAATTG 1 cut(s) 1264
MflI RGATCY 2 cut(s) 541, 2146
MhlI GDGCHC 4 cut(s) 53, 851, 1259, 2417
MlsI TGGCCA 1 cut(s) 1837
MluNI TGGCCA 1 cut(s) 1837
MlyI GAGTC 1 cut(s) 1131
MmeI TCCRAC 5 cut(s) 249, 806, 1294, 1451, 2763
Mox20I TGGCCA 1 cut(s) 1837
Mph1103I ATGCAT 1 cut(s) 397
MroI TCCGGA 1 cut(s) 1714
MroXI GAANNNNTTC 1 cut(s) 2046
MscI TGGCCA 1 cut(s) 1837
MseI TTAA 7 cut(s) 62, 1337, 1883, 1977, 2136, 2319, 2517
MslI CAYNNNNRTG 3 cut(s) 670, 854, 2300
Msp20I TGGCCA 1 cut(s) 1837
MspA1I CMGCKG 1 cut(s) 2031
MspCI CTTAAG 1 cut(s) 2516
MspI CCGG 4 cut(s) 174, 193, 860, 1715
MunI CAATTG 1 cut(s) 1264
MvnI CGCG 2 cut(s) 267, 2031
MwoI GCNNNNNNNGC 7 cut(s) 285, 401, 406, 799, 1358, 1367, 1461
NciI CCSGG 2 cut(s) 193, 861
NdeII GATC 8 cut(s) 183, 541, 559, 1234, 1626, 2146, 2455, 2759
NlaIV GGNNCC 6 cut(s) 543, 688, 984, 2371, 2414, 2787
NmuCI GTSAC 4 cut(s) 733, 1527, 1896, 2387
NruI TCGCGA 1 cut(s) 267
NsiI ATGCAT 1 cut(s) 397
NspI RCATGY 2 cut(s) 746, 1356
PagI TCATGA 2 cut(s) 1479, 2569
PciI ACATGT 1 cut(s) 742
PcsI WCGNNNNNNNCGW 2 cut(s) 24, 2727
PdmI GAANNNNTTC 1 cut(s) 2046
PfeI GAWTC 6 cut(s) 23, 251, 1052, 1385, 1802, 2009
PfoI TCCNGGA 2 cut(s) 712, 2755
PkrI GCNGC 4 cut(s) 119, 190, 1941, 2510
PleI GAGTC 1 cut(s) 1130
PmaCI CACGTG 1 cut(s) 10
PmlI CACGTG 1 cut(s) 10
PpsI GAGTC 1 cut(s) 1130
Ppu21I YACGTR 2 cut(s) 10, 482
PpuMI RGGWCCY 1 cut(s) 474
PscI ACATGT 1 cut(s) 742
PsiI TTATAA 4 cut(s) 1089, 1653, 2538, 2809
Psp124BI GAGCTC 1 cut(s) 1259
Psp5II RGGWCCY 1 cut(s) 474
PspCI CACGTG 1 cut(s) 10
PspEI GGTNACC 1 cut(s) 2387
PspFI CCCAGC 1 cut(s) 1784
PspN4I GGNNCC 6 cut(s) 543, 688, 984, 2371, 2414, 2787
PspPI GGNCC 3 cut(s) 474, 686, 699
PspPPI RGGWCCY 1 cut(s) 474
PsrI GAACNNNNNNTAC 2 cut(s) 2245, 2277
PsuI RGATCY 2 cut(s) 541, 2146
RruI TCGCGA 1 cut(s) 267
RsaI GTAC 7 cut(s) 241, 609, 723, 741, 1662, 2161, 2216
RsaNI GTAC 7 cut(s) 240, 608, 722, 740, 1661, 2160, 2215
RseI CAYNNNNRTG 3 cut(s) 670, 854, 2300
SacI GAGCTC 1 cut(s) 1259
SacII CCGCGG 1 cut(s) 2032
SaqAI TTAA 7 cut(s) 62, 1337, 1883, 1977, 2136, 2319, 2517
SatI GCNGC 4 cut(s) 118, 189, 1940, 2509
Sau3AI GATC 8 cut(s) 183, 541, 559, 1234, 1626, 2146, 2455, 2759
Sau96I GGNCC 3 cut(s) 474, 686, 699
SchI GAGTC 1 cut(s) 1131
SduI GDGCHC 4 cut(s) 53, 851, 1259, 2417
SexAI ACCWGGT 1 cut(s) 985
SfaNI GCATC 3 cut(s) 382, 1847, 2570
SfcI CTRYAG 3 cut(s) 726, 1561, 2448
Sfr303I CCGCGG 1 cut(s) 2032
SgrBI CCGCGG 1 cut(s) 2032
SinI GGWCC 2 cut(s) 474, 686
SmiMI CAYNNNNRTG 3 cut(s) 670, 854, 2300
SmlI CTYRAG 4 cut(s) 1553, 2122, 2516, 2684
SmoI CTYRAG 4 cut(s) 1553, 2122, 2516, 2684
SpeI ACTAGT 1 cut(s) 1166
SsiI CCGC 7 cut(s) 53, 66, 115, 189, 545, 2029, 2031
SspI AATATT 1 cut(s) 772
SspMI CTAG 9 cut(s) 215, 803, 882, 1107, 1167, 1208, 1571, 2157, 2418
SstI GAGCTC 1 cut(s) 1259
StyI CCWWGG 3 cut(s) 1250, 1570, 1664
TaiI ACGT 4 cut(s) 12, 273, 484, 1529
TaqI TCGA 1 cut(s) 579
TatI WGTACW 3 cut(s) 739, 2159, 2214
TauI GCSGC 1 cut(s) 191
TfiI GAWTC 6 cut(s) 23, 251, 1052, 1385, 1802, 2009
Tru1I TTAA 7 cut(s) 62, 1337, 1883, 1977, 2136, 2319, 2517
Tru9I TTAA 7 cut(s) 62, 1337, 1883, 1977, 2136, 2319, 2517
TscAI CASTG 5 cut(s) 301, 385, 610, 800, 902
TseFI GTSAC 4 cut(s) 733, 1527, 1896, 2387
TseI GCWGC 3 cut(s) 117, 1939, 2508
Tsp45I GTSAC 4 cut(s) 733, 1527, 1896, 2387
TspGWI ACGGA 3 cut(s) 16, 1527, 1692
TspRI CASTG 5 cut(s) 301, 385, 610, 800, 902
Vha464I CTTAAG 1 cut(s) 2516
VneI GTGCAC 1 cut(s) 847
VpaK11BI GGWCC 2 cut(s) 474, 686
XagI CCTNNNNNAGG 1 cut(s) 2516
XapI RAATTY 5 cut(s) 946, 962, 1201, 1398, 1500
XceI RCATGY 2 cut(s) 746, 1356
XmaJI CCTAGG 1 cut(s) 1570
XmnI GAANNNNTTC 1 cut(s) 2046
XspI CTAG 9 cut(s) 215, 803, 882, 1107, 1167, 1208, 1571, 2157, 2418
Zsp2I ATGCAT 1 cut(s) 397
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.