MD15G1372700.v1.1

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0000630
Physical Location & Seq
Forward (+)
45518685 .. 45527976
9292 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1372700.v1.1.491

Sequence Viewer

Length: 2928 bp
ATGGAGAATTCTGAGGCCCACGCGTTGGGGCTCACAGATCGCCGGCCGGCCGCTCTCGGAAACTTCCGAGTCCTCCCTGACGACCTCATCTGCGATATCCTGGAATACCTCTCTCCTCGCGACGTCGCTCGCCTCGCTTGCGTCAGCAGTGTGATGTACATACTGTGCAATGAAGAGCCTCTTTGGATGAGTTTATGCCTCGGTAAAGTAAATGGTCCGCTTCAGTATAAGGGCTCGTGGAAAAAGACGGCACTGCATTTAGAGAATGTACCATATGAATGCGACGAGGCTGGCAGAAAACCACTTTATTTTGATGGATTCAACTCCTTGTTCTTGTACAGAAGATTATACAGGTGCCATACCACATTGGATGGTTTTTCTTTTGAAGATGGGAATGTGGCAAGGACGAAGAACCTCGCTATAGAAGACTTCTGTCGTGACTATGATGGGAAGAAACCGGTTTTGCTCACGGGATTAGCTGATGCTTGGCCTGCAAGGCATACATGGACATTTGACCAGTTATTGCAGAAATATGGAGATACAGCTTTCATAATTTCTCAAAAGAGTGCTCGCAAAGTCTCAATGAAATTCAAGGATTATGTTTCATACTTGAAAATTCAACATGATGAGGATCCCCTATATATTTTTGATCACAAGTTTGGAGAGGTTGCACCTGCTTTGTTGAAAGATTACAGTTTACCTTGTCTATTTCAAGAGGACTTTTTTGATGTTTTAGATGGAGATAAACGACCTCCTTTTAGGTGGCTCATTATTGGGCCACAGAGGTCTGGTGCCTCTTGGCATGTTGATCCAGCTCTGACCAGTGCTTGGAACACACTGTTAGTAGGCCGTAAGAGGTGGGCCCTGTATCCTCCAGGAAGAGTACCGCTAGGTGTTACGGTACATGTAAACGAAGAAGATGGTGATGTCAATATTGAAACTCCATCTTCACTGCAGTGGTGGCTAGATTTTTATCCACTTCTTGCTGATGAAGACAAGCCAATAGAGTGTACCCAAGTTCCAGGGGAAACTATTTTTGTTCCAAGTGGATGGTGGCATTGTGTCTTAAATCTGGAACCAAGTATTGCTGTTACCCAGAACTTTGTAAATCCCAAAAACTTTGAATTTGTGTGTTTAGATATGGCACCTGGTTATCGTCATAAGGGAGTTTGCCGTGCTGGATTACTTGCACATGAAGAAGGCATTTCGGAGAATGATTCTATTATCTCTGATCCGATTAGGAAAAAGAAGAGAGTGAGAACTCTCGAACCTGGAGAAAAGAATGCTGATGCTGCCTCTAATGACCGTAATGTACCACAAGGAAGTTACCAGGGTTTTTCTTATGACATCAATTTCTTAGCCATGTATCTGGACAAAGAGAGAGATCACTACAACTCTCCTTGGAGCTCGGGCAATTGCATAGGACAAAGAGAAATGAGAGAATGGTTATTTAAGCTTTGGTGTGGAAAACCAGGAATGAGAGACTTAATCTGGAAGGGAGCTTGTCTTGCATTAAATGCAGGGAGATGGTCCGAATGCCTGGCAGAAATTTGTGCATTCCATAATTTGCCTTCTCCAGCAGAAGATGAGAGGCTTCCTGTTGGCACTGGTAGCAATCCTGTTTATCTCTTGAGCGACTGTGTAGTCAAAATTTTTGTTGAAGAAGGGCTGGAAACGTCACTCTATGGTCTGGGCACAGAGCTAGAGTTTTACAATCTCCTATGCAAAGTCAATTCCCCTCTGAAAAATCACATGCCTGATGTTTTGGAAAGTGGGATCATTTATCTTGAGAATGGAGTTTACAGAATTGTACCATGGGATGGCAACACGGTTCCTGATGTGATTGCCAGATGCAATCTTATTCCTGAGAAGTTCGATGTAGATGGTTGTCCTTGTCCTTTTGGTGTATGGAGTAAAAAACAATTTAATTACAGAAGGGCTGGGATGCCAATACATGAAACTGTCAGCTCACCTGAATGCTCAAGAATATGGCCATATCTTATAACAAAACGATGCAAAGGAAAAATATATGCAGAGTTAAGAGATACCATATCATTGGAAGACGCACTGAACTTAGCATCCTTTCTGGGAGAGCAGCTCCGTAACCTCCATCTCTTGCCCCGGCCACCTCTTAGTGTCTCAACTTTCTCAGATATTGAACCAGAAATAGATATGCCCTTATCTAATGGTTGTATGGAGGCTGTTCCTGATAAATCAAAAATTCCAGCTGAATGGAATATTTTCATCAGGACTCTAATGAGGAAGAAGGATGTCTCAAGCCGCTTGGTTAAATGGGGAGATCCAATTCCTGCCACACTAATAGAGAAAGTCGATGAATACTTACCAGATGATTTTGCCAAGTTGCTTCACATATTTGAGGATGAAAATGGCTTAAACAAAGTGGGTAAACTTTGTTCCTGGATACACTCAGATATCATGGATGACAACATTCACATGGAACCGTGTGGTGCTAATTCTTGCTTGATTGAGAACACCAAAGACAATGGTCTGGTAACCAATGGTTCAGTGAATGGAAATGGCAACAGTGCAGGGACTGAGTCATGGCGTCCTAGCCACATTCTTGATTTCAGTGATCTTTCTATAGGTGACCCCATTTTTGATATAATACCCATATACTTGGATATATTTAGAGGTGATACGCATCTCCTTAAGCGGCTTTTAGAAAGCTATAAAGTTCCTTTAGTGAGTGGAGAATCACAGAATAAGTCTGTTAAGGGTGGAGACAAGTTTGGACGGCTATCCTACCATGCGATGTGCTACTGTATTATGCATGAAGAGAATGTGTTGGGAGCTATTTTTAGCATCTGGAGCGAACTCAAGACGGCAAAGACATGGGAAGAAGTTGAGCAAGCGGTGTGGGGAGAGTTGAATAATTACAAAGGTTTCCCTGGTTTATCTTCCACTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
APH Cupin_8 F-box F-box-like JmjC

Protein Analysis

976

Amino Acids

110.42

Weight (kDa)

5.29

Isoelectric Point (pI)

45.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box-like PF12937 24 - 67 8.5e-10 F-box-like
F-box PF00646 25 - 62 3.5e-07 F-box domain
Cupin_8 PF13621 151 - 370 4.1e-19 Cupin-like domain
JmjC PF02373 255 - 368 3.8e-14 JmjC domain, hydroxylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000630)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G78280
fragaria_vesca FvH4_5g36800 FvH4_5g36800 FvH4_5g36800 FvH4_5g36800 FvH4_5g36800 FvH4_5g36800 FvH4_5g36800 FvH4_5g36800 FvH4_5g36800 FvH4_5g36800 FvH4_5g36800 FvH4_5g36800 FvH4_5g36800 FvH4_5g36800 FvH4_5g36800 FvH4_5g36800 FvH4_5g36800 FvH4_5g36800
malus_domestica MD08G1186800.v1.1 MD15G1372700.v1.1
prunus_persica Prupe.1G521300_v2.0.a1 Prupe.1G521300_v2.0.a1
pyrus_communis pycom08g16060 pycom15g33370
rosa_chinensis RchiOBHm_Chr2g0139391 RchiOBHm_Chr7g0242931 RchiOBHm_Chr7g0242951 RchiOBHm_Chr7g0243001 RchiOBHm_Chr7g0243011 RchiOBHm_Chr7g0243141 RchiOBHm_Chr7g0243151 RchiOBHm_Chr7g0243351
rosa_laevigata RLG00000000853 RLG00000000857 RLG00000000858 RLG00000000863 RLG00000000867
rosa_multiflora Rmu_co8457323.1_g000001 Rmu_sc0000083.1_g000017 Rmu_sc0000679.1_g000021 Rmu_sc0001036.1_g000029 Rmu_sc0001036.1_g000032 Rmu_sc0001176.1_g000015 Rmu_sc0001461.1_g000002 Rmu_sc0001461.1_g000003 Rmu_sc0001461.1_g000056 Rmu_sc0001461.1_g000057 Rmu_sc0001461.1_g000098 Rmu_sc0001461.1_g000105 Rmu_sc0004165.1_g000084 Rmu_sc0006141.1_g000004 Rmu_sc0033806.1_g000001
rosa_roxburghii Rroxscaffold_3G00222160 Rroxscaffold_3G00222220 Rroxscaffold_4G00316290
rosa_rugosa Rorug07G0318400 Rorug07G0318400 Rorug07G0318600
rosa_samantha Rh4CG066500 Rh5DG444300 Rh7AG473700 Rh7AG473900 Rh7AG474000 Rh7AG474600 Rh7AG474800 Rh7BG444500 Rh7BG444700 Rh7CG490700 Rh7CG490800 Rh7CG491000 Rh7DG458900 Rh7DG459000 Rh7DG459300
rosa_wichuraiana Rw7G038970 Rw7G039050 Rw7G039420 Rw7G039480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2003
AarI CACCTGC 1 cut(s) 682
AasI GACNNNNNNGTC 1 cut(s) 1643
AatII GACGTC 1 cut(s) 126
Acc36I ACCTGC 1 cut(s) 682
AccB1I GGYRCC 3 cut(s) 354, 791, 1144
AccB7I CCANNNNNTGG 3 cut(s) 25, 828, 1820
AccBSI CCGCTC 1 cut(s) 53
AccII CGCG 2 cut(s) 23, 120
AciI CCGC 6 cut(s) 51, 218, 887, 2281, 2674, 2873
AclWI GGATC 6 cut(s) 626, 639, 803, 1226, 1784, 2294
AcoI YGGCCR 4 cut(s) 44, 48, 1991, 2123
AcsI RAATTY 7 cut(s) 7, 587, 615, 1124, 1548, 1650, 2220
AcuI CTGAAG 1 cut(s) 206
AcyI GRCGYC 2 cut(s) 123, 2566
AfaI GTAC 8 cut(s) 158, 270, 338, 885, 903, 1012, 1314, 1812
AfiI CCNNNNNNNGG 3 cut(s) 25, 828, 1820
AflII CTTAAG 1 cut(s) 2669
AflIII ACRYGT 2 cut(s) 21, 904
AgeI ACCGGT 1 cut(s) 457
AleI CACNNNNGTG 1 cut(s) 955
AloI GAACNNNNNNTCC 2 cut(s) 2063, 2095
Alw21I GWGCWC 2 cut(s) 571, 1409
Alw26I GTCTC 5 cut(s) 583, 1476, 2143, 2278, 2736
AlwI GGATC 6 cut(s) 626, 639, 803, 1226, 1784, 2294
AlwNI CAGNNNCTG 1 cut(s) 2555
Ama87I CYCGRG 1 cut(s) 1408
AoxI GGCC 9 cut(s) 15, 44, 48, 488, 776, 847, 861, 1991, 2123
ApaI GGGCCC 1 cut(s) 865
ApeKI GCWGC 2 cut(s) 1292, 2095
ApoI RAATTY 7 cut(s) 7, 587, 615, 1124, 1548, 1650, 2220
ArsI GACNNNNNNTTYG 2 cut(s) 2600, 2632
AsiGI ACCGGT 1 cut(s) 457
Asp700I GAANNNNTTC 1 cut(s) 2240
AspS9I GGNCC 6 cut(s) 16, 215, 776, 861, 862, 1530
AsuC2I CCSGG 1 cut(s) 2122
AsuHPI GGTGA 4 cut(s) 935, 1962, 2618, 2666
AvaI CYCGRG 1 cut(s) 1408
AvaII GGWCC 2 cut(s) 215, 1530
BaeGI GKGCMC 2 cut(s) 865, 1697
BalI TGGCCA 1 cut(s) 1993
BamHI GGATCC 1 cut(s) 631
BanI GGYRCC 3 cut(s) 354, 791, 1144
BanII GRGCYC 4 cut(s) 33, 236, 865, 1409
BauI CACGAG 1 cut(s) 235
BbsI GAAGAC 3 cut(s) 432, 999, 2067
Bbv12I GWGCWC 2 cut(s) 571, 1409
BbvI GCAGC 2 cut(s) 1279, 2107
BceAI ACGGC 5 cut(s) 264, 834, 1158, 2771, 2859
BciVI GTATCC 2 cut(s) 879, 2415
BclI TGATCA 1 cut(s) 649
BcnI CCSGG 1 cut(s) 2122
BcoDI GTCTC 5 cut(s) 583, 1476, 2143, 2278, 2736
BfaI CTAG 4 cut(s) 890, 965, 1703, 2571
BfmI CTRYAG 3 cut(s) 420, 953, 2601
BfrI CTTAAG 1 cut(s) 2669
BfuAI ACCTGC 1 cut(s) 682
BfuI GTATCC 2 cut(s) 879, 2415
BglI GCCNNNNNGGC 1 cut(s) 496
BisI GCNGC 5 cut(s) 51, 1293, 2096, 2281, 2675
BlsI GCNGC 5 cut(s) 52, 1294, 2097, 2282, 2676
Bme18I GGWCC 2 cut(s) 215, 1530
BmeT110I CYCGRG 1 cut(s) 1408
BmgT120I GGNCC 6 cut(s) 16, 215, 776, 861, 862, 1530
BmiI GGNNCC 8 cut(s) 356, 633, 793, 863, 1077, 1146, 1833, 2460
BmsI GCATC 8 cut(s) 472, 1279, 1841, 1935, 2003, 2087, 2671, 2832
BoxI GACNNNNGTC 2 cut(s) 432, 2505
BpiI GAAGAC 3 cut(s) 432, 999, 2067
BplI GAGNNNNNCTC 2 cut(s) 2082, 2114
BpmI CTGGAG 4 cut(s) 858, 1293, 1560, 2848
BpuEI CTTGAG 5 cut(s) 1651, 1808, 1966, 2260, 2822
BpuMI CCSGG 1 cut(s) 2122
BsaBI GATNNNNATC 3 cut(s) 630, 972, 2661
BsaHI GRCGYC 2 cut(s) 123, 2566
BsaJI CCNNGG 7 cut(s) 199, 1022, 1330, 1400, 1814, 2120, 2908
BsaWI WCCGGW 1 cut(s) 457
Bsc4I CCNNNNNNNGG 3 cut(s) 25, 828, 1820
Bse118I RCCGGY 3 cut(s) 42, 46, 457
Bse1I ACTGG 3 cut(s) 517, 822, 1612
Bse3DI GCAATG 1 cut(s) 175
Bse8I GATNNNNATC 3 cut(s) 630, 972, 2661
BseDI CCNNGG 7 cut(s) 199, 1022, 1330, 1400, 1814, 2120, 2908
BseGI GGATG 9 cut(s) 192, 376, 1055, 1825, 1950, 2078, 2275, 2386, 2446
BseJI GATNNNNATC 3 cut(s) 630, 972, 2661
BseLI CCNNNNNNNGG 3 cut(s) 25, 828, 1820
BseMI GCAATG 1 cut(s) 175
BseMII CTCAG 4 cut(s) 1857, 2163, 2442, 2547
BseNI ACTGG 3 cut(s) 517, 822, 1612
BseRI GAGGAG 1 cut(s) 105
BseSI GKGCMC 2 cut(s) 865, 1697
BseX3I CGGCCG 2 cut(s) 44, 48
BseXI GCAGC 2 cut(s) 1279, 2107
BseYI CCCAGC 1 cut(s) 1940
BsgI GTGCAG 1 cut(s) 2568
Bsh1236I CGCG 2 cut(s) 23, 120
Bsh1285I CGRYCG 2 cut(s) 47, 51
BshFI GGCC 9 cut(s) 17, 46, 50, 490, 778, 849, 863, 1993, 2125
BshNI GGYRCC 3 cut(s) 354, 791, 1144
BshTI ACCGGT 1 cut(s) 457
BsiEI CGRYCG 2 cut(s) 47, 51
BsiHKAI GWGCWC 2 cut(s) 571, 1409
BsiHKCI CYCGRG 1 cut(s) 1408
BsiSI CCGG 4 cut(s) 43, 47, 458, 2122
BslFI GGGAC 1 cut(s) 2566
BslI CCNNNNNNNGG 3 cut(s) 25, 828, 1820
BsmAI GTCTC 5 cut(s) 583, 1476, 2143, 2278, 2736
BsmFI GGGAC 1 cut(s) 2566
BsmI GAATGC 5 cut(s) 284, 1288, 1541, 1556, 1982
BsnI GGCC 9 cut(s) 17, 46, 50, 490, 778, 849, 863, 1993, 2125
BsoBI CYCGRG 1 cut(s) 1408
Bsp120I GGGCCC 1 cut(s) 861
Bsp1286I GDGCHC 6 cut(s) 33, 236, 571, 865, 1409, 1697
Bsp1407I TGTACA 2 cut(s) 156, 336
Bsp143I GATC 9 cut(s) 37, 631, 649, 808, 1231, 1384, 1776, 2299, 2593
Bsp19I CCATGG 1 cut(s) 1814
Bsp68I TCGCGA 1 cut(s) 120
BspACI CCGC 6 cut(s) 51, 218, 887, 2281, 2674, 2873
BspANI GGCC 9 cut(s) 17, 46, 50, 490, 778, 849, 863, 1993, 2125
BspCNI CTCAG 5 cut(s) 4, 1858, 2162, 2441, 2548
BspFNI CGCG 2 cut(s) 23, 120
BspLI GGNNCC 8 cut(s) 356, 633, 793, 863, 1077, 1146, 1833, 2460
BspMAI CTGCAG 1 cut(s) 957
BspMI ACCTGC 1 cut(s) 682
BspPI GGATC 6 cut(s) 626, 639, 803, 1226, 1784, 2294
BspQI GCTCTTC 1 cut(s) 168
BspT107I GGYRCC 3 cut(s) 354, 791, 1144
BspTI CTTAAG 1 cut(s) 2669
BsrBI CCGCTC 1 cut(s) 53
BsrDI GCAATG 1 cut(s) 175
BsrFI RCCGGY 3 cut(s) 42, 46, 457
BsrGI TGTACA 2 cut(s) 156, 336
BsrI ACTGG 3 cut(s) 517, 822, 1612
BssAI RCCGGY 3 cut(s) 42, 46, 457
BssECI CCNNGG 7 cut(s) 199, 1022, 1330, 1400, 1814, 2120, 2908
BssMI GATC 9 cut(s) 37, 631, 649, 808, 1231, 1384, 1776, 2299, 2593
BssNI GRCGYC 2 cut(s) 123, 2566
BssSI CACGAG 1 cut(s) 235
BssT1I CCWWGG 2 cut(s) 1400, 1814
Bst2BI CACGAG 1 cut(s) 235
Bst6I CTCTTC 4 cut(s) 168, 874, 1244, 2790
BstACI GRCGYC 2 cut(s) 123, 2566
BstAFI CTTAAG 1 cut(s) 2669
BstAPI GCANNNNNTGC 1 cut(s) 1517
BstAUI TGTACA 2 cut(s) 156, 336
BstC8I GCNNGC 8 cut(s) 44, 48, 130, 139, 292, 492, 571, 2871
BstDEI CTNAG 9 cut(s) 12, 1357, 1866, 2074, 2132, 2149, 2428, 2556, 2925
BstDSI CCRYGG 1 cut(s) 1814
BstEII GGTNACC 2 cut(s) 2512, 2606
BstF5I GGATG 9 cut(s) 192, 376, 1055, 1825, 1950, 2078, 2275, 2386, 2446
BstFNI CGCG 2 cut(s) 23, 120
BstKTI GATC 9 cut(s) 40, 634, 652, 811, 1234, 1387, 1779, 2302, 2596
BstMAI GTCTC 5 cut(s) 583, 1476, 2143, 2278, 2736
BstMBI GATC 9 cut(s) 37, 631, 649, 808, 1231, 1384, 1776, 2299, 2593
BstMCI CGRYCG 2 cut(s) 47, 51
BstNSI RCATGY 3 cut(s) 806, 908, 1756
BstPAI GACNNNNGTC 2 cut(s) 432, 2505
BstPI GGTNACC 2 cut(s) 2512, 2606
BstSFI CTRYAG 3 cut(s) 420, 953, 2601
BstSLI GKGCMC 2 cut(s) 865, 1697
BstUI CGCG 2 cut(s) 23, 120
BstV1I GCAGC 2 cut(s) 1279, 2107
BstV2I GAAGAC 3 cut(s) 432, 999, 2067
BstX2I RGATCY 2 cut(s) 631, 2299
BstXI CCANNNNNNTGG 5 cut(s) 1050, 1369, 2056, 2232, 2638
BstYI RGATCY 2 cut(s) 631, 2299
BstZI CGGCCG 2 cut(s) 44, 48
BsuI GTATCC 2 cut(s) 879, 2415
BsuRI GGCC 9 cut(s) 17, 46, 50, 490, 778, 849, 863, 1993, 2125
BtgI CCRYGG 1 cut(s) 1814
BtgZI GCGATG 1 cut(s) 2786
BtsCI GGATG 9 cut(s) 192, 376, 1055, 1825, 1950, 2078, 2275, 2386, 2446
BtsI GCAGTG 4 cut(s) 154, 251, 950, 962
BtuMI TCGCGA 1 cut(s) 120
BveI ACCTGC 1 cut(s) 682
Cac8I GCNNGC 8 cut(s) 44, 48, 130, 139, 292, 492, 571, 2871
CaiI CAGNNNCTG 1 cut(s) 2555
Cfr10I RCCGGY 3 cut(s) 42, 46, 457
Cfr13I GGNCC 6 cut(s) 16, 215, 776, 861, 862, 1530
CseI GACGC 3 cut(s) 130, 2072, 2555
CsiI ACCWGGT 1 cut(s) 1147
Csp6I GTAC 8 cut(s) 157, 269, 337, 884, 902, 1011, 1313, 1811
CspAI ACCGGT 1 cut(s) 457
CspCI CAANNNNNGTGG 2 cut(s) 2858, 2893
CviQI GTAC 8 cut(s) 157, 269, 337, 884, 902, 1011, 1313, 1811
DdeI CTNAG 9 cut(s) 12, 1357, 1866, 2074, 2132, 2149, 2428, 2556, 2925
DpnI GATC 9 cut(s) 39, 633, 651, 810, 1233, 1386, 1778, 2301, 2595
DpnII GATC 9 cut(s) 37, 631, 649, 808, 1231, 1384, 1776, 2299, 2593
DrdI GACNNNNNNGTC 1 cut(s) 1643
DseDI GACNNNNNNGTC 1 cut(s) 1643
EaeI YGGCCR 4 cut(s) 44, 48, 1991, 2123
EagI CGGCCG 2 cut(s) 44, 48
Eam1104I CTCTTC 4 cut(s) 168, 874, 1244, 2790
EarI CTCTTC 4 cut(s) 168, 874, 1244, 2790
Ecl136II GAGCTC 1 cut(s) 1407
EclXI CGGCCG 2 cut(s) 44, 48
Eco130I CCWWGG 2 cut(s) 1400, 1814
Eco24I GRGCYC 4 cut(s) 33, 236, 865, 1409
Eco32I GATATC 2 cut(s) 97, 2434
Eco47I GGWCC 2 cut(s) 215, 1530
Eco52I CGGCCG 2 cut(s) 44, 48
Eco53kI GAGCTC 1 cut(s) 1407
Eco57I CTGAAG 1 cut(s) 206
Eco88I CYCGRG 1 cut(s) 1408
Eco91I GGTNACC 2 cut(s) 2512, 2606
EcoICRI GAGCTC 1 cut(s) 1407
EcoO109I RGGNCCY 1 cut(s) 862
EcoO65I GGTNACC 2 cut(s) 2512, 2606
EcoRI GAATTC 1 cut(s) 7
EcoRV GATATC 2 cut(s) 97, 2434
EcoT14I CCWWGG 2 cut(s) 1400, 1814
EcoT22I ATGCAT 1 cut(s) 2793
EcoT38I GRGCYC 4 cut(s) 33, 236, 865, 1409
ErhI CCWWGG 2 cut(s) 1400, 1814
FalI AAGNNNNNCTT 2 cut(s) 165, 197
FaqI GGGAC 1 cut(s) 2566
FauNDI CATATG 1 cut(s) 274
FbaI TGATCA 1 cut(s) 649
Fnu4HI GCNGC 5 cut(s) 51, 1293, 2096, 2281, 2675
FokI GGATG 9 cut(s) 199, 383, 1062, 1832, 1957, 2065, 2282, 2393, 2453
FriOI GRGCYC 4 cut(s) 33, 236, 865, 1409
FseI GGCCGGCC 1 cut(s) 50
Fsp4HI GCNGC 5 cut(s) 51, 1293, 2096, 2281, 2675
FspBI CTAG 4 cut(s) 890, 965, 1703, 2571
GluI GCNGC 5 cut(s) 51, 1293, 2096, 2281, 2675
GsaI CCCAGC 1 cut(s) 1944
GsuI CTGGAG 4 cut(s) 858, 1293, 1560, 2848
HaeIII GGCC 9 cut(s) 17, 46, 50, 490, 778, 849, 863, 1993, 2125
HapII CCGG 4 cut(s) 43, 47, 458, 2122
HgaI GACGC 3 cut(s) 130, 2072, 2555
Hin1I GRCGYC 2 cut(s) 123, 2566
HindIII AAGCTT 1 cut(s) 1454
HinfI GANTC 6 cut(s) 69, 318, 1217, 2251, 2558, 2714
HpaII CCGG 4 cut(s) 43, 47, 458, 2122
HphI GGTGA 4 cut(s) 935, 1962, 2618, 2666
Hpy166II GTNNAC 5 cut(s) 698, 910, 1011, 1801, 2408
Hpy8I GTNNAC 5 cut(s) 698, 910, 1011, 1801, 2408
Hpy99I CGWCG 3 cut(s) 125, 128, 287
HpyAV CCTTC 6 cut(s) 1193, 1489, 1581, 1658, 1929, 2260
HpyCH4IV ACGT 2 cut(s) 123, 1676
HpyF3I CTNAG 9 cut(s) 12, 1357, 1866, 2074, 2132, 2149, 2428, 2556, 2925
HpySE526I ACGT 2 cut(s) 123, 1676
Hsp92I GRCGYC 2 cut(s) 123, 2566
KroI GCCGGC 2 cut(s) 42, 46
KroNI GCCGGC 2 cut(s) 44, 48
Ksp22I TGATCA 1 cut(s) 649
Kzo9I GATC 9 cut(s) 37, 631, 649, 808, 1231, 1384, 1776, 2299, 2593
LguI GCTCTTC 1 cut(s) 168
LmnI GCTCC 5 cut(s) 1404, 1499, 2103, 2810, 2829
Lsp1109I GCAGC 2 cut(s) 1279, 2107
LweI GCATC 8 cut(s) 472, 1279, 1841, 1935, 2003, 2087, 2671, 2832
MabI ACCWGGT 1 cut(s) 1147
MaeI CTAG 4 cut(s) 890, 965, 1703, 2571
MaeII ACGT 2 cut(s) 123, 1676
MaeIII GTNAC 8 cut(s) 437, 895, 1090, 1325, 1677, 2102, 2512, 2606
MalI GATC 9 cut(s) 39, 633, 651, 810, 1233, 1386, 1778, 2301, 2595
MbiI CCGCTC 1 cut(s) 53
MboI GATC 9 cut(s) 37, 631, 649, 808, 1231, 1384, 1776, 2299, 2593
MfeI CAATTG 1 cut(s) 1414
MflI RGATCY 2 cut(s) 631, 2299
MhlI GDGCHC 6 cut(s) 33, 236, 571, 865, 1409, 1697
MlsI TGGCCA 1 cut(s) 1993
MluI ACGCGT 1 cut(s) 21
MluNI TGGCCA 1 cut(s) 1993
MlyI GAGTC 3 cut(s) 78, 2245, 2567
Mox20I TGGCCA 1 cut(s) 1993
Mph1103I ATGCAT 1 cut(s) 2793
MroNI GCCGGC 2 cut(s) 42, 46
MroXI GAANNNNTTC 1 cut(s) 2240
MscI TGGCCA 1 cut(s) 1993
MslI CAYNNNNRTG 4 cut(s) 277, 955, 1975, 2453
Msp20I TGGCCA 1 cut(s) 1993
MspA1I CMGCKG 1 cut(s) 2228
MspCI CTTAAG 1 cut(s) 2669
MspI CCGG 4 cut(s) 43, 47, 458, 2122
MunI CAATTG 1 cut(s) 1414
Mva1269I GAATGC 5 cut(s) 284, 1288, 1541, 1556, 1982
MvnI CGCG 2 cut(s) 23, 120
NaeI GCCGGC 2 cut(s) 44, 48
NciI CCSGG 1 cut(s) 2122
NcoI CCATGG 1 cut(s) 1814
NdeI CATATG 1 cut(s) 274
NdeII GATC 9 cut(s) 37, 631, 649, 808, 1231, 1384, 1776, 2299, 2593
NgoMIV GCCGGC 2 cut(s) 42, 46
NlaIV GGNNCC 8 cut(s) 356, 633, 793, 863, 1077, 1146, 1833, 2460
NmuCI GTSAC 3 cut(s) 437, 1677, 2606
NruI TCGCGA 1 cut(s) 120
NsiI ATGCAT 1 cut(s) 2793
NspI RCATGY 3 cut(s) 806, 908, 1756
OliI CACNNNNGTG 1 cut(s) 955
PaqCI CACCTGC 1 cut(s) 682
PciI ACATGT 1 cut(s) 904
PciSI GCTCTTC 1 cut(s) 168
PctI GAATGC 5 cut(s) 284, 1288, 1541, 1556, 1982
PdiI GCCGGC 2 cut(s) 44, 48
PdmI GAANNNNTTC 1 cut(s) 2240
PfeI GAWTC 3 cut(s) 318, 1217, 2714
PflFI GACNNNGTC 1 cut(s) 2557
PflMI CCANNNNNTGG 3 cut(s) 25, 828, 1820
PfoI TCCNGGA 3 cut(s) 99, 874, 2417
PinAI ACCGGT 1 cut(s) 457
PkrI GCNGC 5 cut(s) 52, 1294, 2097, 2282, 2676
PleI GAGTC 3 cut(s) 77, 2245, 2566
PpsI GAGTC 3 cut(s) 77, 2245, 2566
PscI ACATGT 1 cut(s) 904
PshAI GACNNNNGTC 2 cut(s) 432, 2505
PsiI TTATAA 1 cut(s) 2003
Psp124BI GAGCTC 1 cut(s) 1409
PspEI GGTNACC 2 cut(s) 2512, 2606
PspFI CCCAGC 1 cut(s) 1940
PspN4I GGNNCC 8 cut(s) 356, 633, 793, 863, 1077, 1146, 1833, 2460
PspOMI GGGCCC 1 cut(s) 861
PspPI GGNCC 6 cut(s) 16, 215, 776, 861, 862, 1530
PsrI GAACNNNNNNTAC 2 cut(s) 2398, 2430
PstI CTGCAG 1 cut(s) 957
PstNI CAGNNNCTG 1 cut(s) 2555
PsuI RGATCY 2 cut(s) 631, 2299
PsyI GACNNNGTC 1 cut(s) 2557
PvuII CAGCTG 1 cut(s) 2228
RigI GGCCGGCC 1 cut(s) 50
RruI TCGCGA 1 cut(s) 120
RsaI GTAC 8 cut(s) 158, 270, 338, 885, 903, 1012, 1314, 1812
RsaNI GTAC 8 cut(s) 157, 269, 337, 884, 902, 1011, 1313, 1811
RseI CAYNNNNRTG 4 cut(s) 277, 955, 1975, 2453
SacI GAGCTC 1 cut(s) 1409
SapI GCTCTTC 1 cut(s) 168
SatI GCNGC 5 cut(s) 51, 1293, 2096, 2281, 2675
Sau3AI GATC 9 cut(s) 37, 631, 649, 808, 1231, 1384, 1776, 2299, 2593
Sau96I GGNCC 6 cut(s) 16, 215, 776, 861, 862, 1530
SchI GAGTC 3 cut(s) 78, 2245, 2567
SduI GDGCHC 6 cut(s) 33, 236, 571, 865, 1409, 1697
SexAI ACCWGGT 1 cut(s) 1147
SfaNI GCATC 8 cut(s) 472, 1279, 1841, 1935, 2003, 2087, 2671, 2832
SfcI CTRYAG 3 cut(s) 420, 953, 2601
SinI GGWCC 2 cut(s) 215, 1530
SmiMI CAYNNNNRTG 4 cut(s) 277, 955, 1975, 2453
SmlI CTYRAG 6 cut(s) 1630, 1787, 1981, 2275, 2669, 2837
SmoI CTYRAG 6 cut(s) 1630, 1787, 1981, 2275, 2669, 2837
SsiI CCGC 6 cut(s) 51, 218, 887, 2281, 2674, 2873
SspI AATATT 2 cut(s) 934, 2239
SspMI CTAG 4 cut(s) 890, 965, 1703, 2571
SstI GAGCTC 1 cut(s) 1409
StyI CCWWGG 2 cut(s) 1400, 1814
TaiI ACGT 2 cut(s) 126, 1679
TaqI TCGA 3 cut(s) 1266, 1875, 2331
TatI WGTACW 2 cut(s) 156, 336
TauI GCSGC 3 cut(s) 53, 2283, 2677
TfiI GAWTC 3 cut(s) 318, 1217, 2714
TseFI GTSAC 3 cut(s) 437, 1677, 2606
TseI GCWGC 2 cut(s) 1292, 2095
Tsp45I GTSAC 3 cut(s) 437, 1677, 2606
TspGWI ACGGA 1 cut(s) 2090
Tth111I GACNNNGTC 1 cut(s) 2557
Van91I CCANNNNNTGG 3 cut(s) 25, 828, 1820
Vha464I CTTAAG 1 cut(s) 2669
VpaK11BI GGWCC 2 cut(s) 215, 1530
XapI RAATTY 7 cut(s) 7, 587, 615, 1124, 1548, 1650, 2220
XceI RCATGY 3 cut(s) 806, 908, 1756
XcmI CCANNNNNNNNNTGG 1 cut(s) 1050
XmnI GAANNNNTTC 1 cut(s) 2240
XspI CTAG 4 cut(s) 890, 965, 1703, 2571
ZraI GACGTC 1 cut(s) 124
Zsp2I ATGCAT 1 cut(s) 2793
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.