AT3G43850

RNA binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0000728
Physical Location & Seq
Reverse (-)
15702085 .. 15704391
2307 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G43850.2

Sequence Viewer

Length: 813 bp
ATGTTTACTGCCATTGACAAGATTGATCGGACTTTCACGGTGGATCAAGAATTTGCTTGCTTATCGTCGTCGACGTCTTCTGATTCGATCGGGGAGAACAGTGACGACGATGAAGGTGGAGAGAATGAGATTGAGTCTTCTTACAATGGTCCTCTTGATATGATGGAATCTCTTGAAGAAGCCTTGCCCATCAAGAGAGCTATATCAAAGTTTTACAAAGGGAAGTCAAAGTCGTTCATGAGCTTATCGGAAACTTCATCTTTGCCGGTGAAGGACTTGACGAAACCGGAGAATCTTTATAGTCGTCGGAGGAGGAATCTTCTCAGTCACCGGATATGTAGTCGTGGTGGGATCTCAAAGAAGCCATTCAAGAGTGTTTTAGCGATGAGTCAACGAGAAGGAGACTCTTCTTCCTCCGGTGATGATTCTCTTCCGACTCTAAGGCAGCATCATAAGACTCTTACGCCGAGGCTGAGAAAAGGGAGTTTTGAAGCTTTTACATTTCAAGACAAAAGCGAGACGGATGAGGATCACAAGAAGGCCACCAAAGGAGCCACAAAGCCTGTGAATCAAACCTGGTCAAGCTCTGGTCACAGTATAAAGACAAGGCAATGGCCAAGATCAACAATAGCAGAAGCAAGAAGAGCCGGTGAAAGCATGGCTAGTATTGACAAGCTGATGGCCGAAAACTTCCTGGATAATGTAAACCTTGATCCTATTTCCATCAAGAAGTCGAGAGATCCTTGTGTTCCTAAATCTTCTTGTTGTTTGTTTCTCAACACTTCTCTGTATTTCTTGGGAAGGACTGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

270

Amino Acids

30.14

Weight (kDa)

8.5

Isoelectric Point (pI)

54.17

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 77
AccI GTMKAC 1 cut(s) 71
AclWI GGATC 5 cut(s) 51, 359, 537, 707, 734
AcoI YGGCCR 2 cut(s) 614, 681
AcsI RAATTY 1 cut(s) 50
AcyI GRCGYC 1 cut(s) 74
AgsI TTSAA 4 cut(s) 176, 370, 491, 506
AjnI CCWGG 2 cut(s) 575, 693
AluBI AGCT 5 cut(s) 200, 243, 494, 585, 676
AluI AGCT 5 cut(s) 200, 243, 494, 585, 676
Alw26I GTCTC 2 cut(s) 396, 512
AlwI GGATC 5 cut(s) 51, 359, 537, 707, 734
AoxI GGCC 3 cut(s) 540, 614, 681
ApeKI GCWGC 1 cut(s) 445
ApoI RAATTY 1 cut(s) 50
Asp700I GAANNNNTTC 1 cut(s) 365
AspS9I GGNCC 1 cut(s) 149
AsuHPI GGTGA 4 cut(s) 280, 320, 431, 662
AvaII GGWCC 1 cut(s) 149
BalI TGGCCA 1 cut(s) 616
BbsI GAAGAC 2 cut(s) 69, 129
BbvI GCAGC 1 cut(s) 457
BccI CCATC 4 cut(s) 157, 197, 673, 731
BciT130I CCWGG 2 cut(s) 577, 695
BcoDI GTCTC 2 cut(s) 396, 512
BfaI CTAG 1 cut(s) 663
BisI GCNGC 1 cut(s) 446
BlsI GCNGC 1 cut(s) 447
Bme1390I CCNGG 2 cut(s) 577, 695
Bme18I GGWCC 1 cut(s) 149
BmgT120I GGNCC 1 cut(s) 149
BmiI GGNNCC 1 cut(s) 553
BmrFI CCNGG 2 cut(s) 577, 695
BmsI GCATC 1 cut(s) 457
BpiI GAAGAC 2 cut(s) 69, 129
BsaBI GATNNNNATC 1 cut(s) 528
BsaHI GRCGYC 1 cut(s) 74
BsaJI CCNNGG 1 cut(s) 467
BsaWI WCCGGW 3 cut(s) 286, 330, 416
Bse118I RCCGGY 2 cut(s) 265, 647
Bse3DI GCAATG 1 cut(s) 617
Bse8I GATNNNNATC 1 cut(s) 528
BseBI CCWGG 2 cut(s) 577, 695
BseDI CCNNGG 1 cut(s) 467
BseGI GGATG 1 cut(s) 529
BseJI GATNNNNATC 1 cut(s) 528
BseMI GCAATG 1 cut(s) 617
BseMII CTCAG 2 cut(s) 337, 464
BseRI GAGGAG 1 cut(s) 325
BseXI GCAGC 1 cut(s) 457
Bsh1285I CGRYCG 1 cut(s) 90
BshFI GGCC 3 cut(s) 542, 616, 683
BsiEI CGRYCG 1 cut(s) 90
BsiSI CCGG 5 cut(s) 266, 287, 331, 417, 648
BsmAI GTCTC 2 cut(s) 396, 512
BsmBI CGTCTC 1 cut(s) 512
BsnI GGCC 3 cut(s) 542, 616, 683
Bsp143I GATC 8 cut(s) 25, 43, 87, 351, 529, 620, 712, 739
BspANI GGCC 3 cut(s) 542, 616, 683
BspCNI CTCAG 2 cut(s) 336, 465
BspHI TCATGA 1 cut(s) 237
BspLI GGNNCC 1 cut(s) 553
BspPI GGATC 5 cut(s) 51, 359, 537, 707, 734
BspQI GCTCTTC 1 cut(s) 637
BsrDI GCAATG 1 cut(s) 617
BsrFI RCCGGY 2 cut(s) 265, 647
BssAI RCCGGY 2 cut(s) 265, 647
BssECI CCNNGG 1 cut(s) 467
BssMI GATC 8 cut(s) 25, 43, 87, 351, 529, 620, 712, 739
BssNI GRCGYC 1 cut(s) 74
Bst2UI CCWGG 2 cut(s) 577, 695
Bst4CI ACNGT 3 cut(s) 40, 101, 596
Bst6I CTCTTC 3 cut(s) 412, 435, 637
BstACI GRCGYC 1 cut(s) 74
BstC8I GCNNGC 1 cut(s) 58
BstDEI CTNAG 3 cut(s) 323, 440, 473
BstF5I GGATG 1 cut(s) 529
BstKTI GATC 8 cut(s) 28, 46, 90, 354, 532, 623, 715, 742
BstMAI GTCTC 2 cut(s) 396, 512
BstMBI GATC 8 cut(s) 25, 43, 87, 351, 529, 620, 712, 739
BstMCI CGRYCG 1 cut(s) 90
BstMWI GCNNNNNNNGC 1 cut(s) 644
BstNI CCWGG 2 cut(s) 577, 695
BstSCI CCNGG 2 cut(s) 575, 693
BstV1I GCAGC 1 cut(s) 457
BstV2I GAAGAC 2 cut(s) 69, 129
BstX2I RGATCY 2 cut(s) 351, 739
BstYI RGATCY 2 cut(s) 351, 739
BsuRI GGCC 3 cut(s) 542, 616, 683
BtgZI GCGATG 1 cut(s) 398
BtsCI GGATG 1 cut(s) 529
BtsIMutI CAGTG 1 cut(s) 106
Cac8I GCNNGC 1 cut(s) 58
CciI TCATGA 1 cut(s) 237
Cfr10I RCCGGY 2 cut(s) 265, 647
Cfr13I GGNCC 1 cut(s) 149
CsiI ACCWGGT 1 cut(s) 575
CviAII CATG 2 cut(s) 238, 658
DdeI CTNAG 3 cut(s) 323, 440, 473
DpnI GATC 8 cut(s) 27, 45, 89, 353, 531, 622, 714, 741
DpnII GATC 8 cut(s) 25, 43, 87, 351, 529, 620, 712, 739
EaeI YGGCCR 2 cut(s) 614, 681
Eam1104I CTCTTC 3 cut(s) 412, 435, 637
EarI CTCTTC 3 cut(s) 412, 435, 637
Eco47I GGWCC 1 cut(s) 149
EcoRII CCWGG 2 cut(s) 575, 693
Esp3I CGTCTC 1 cut(s) 512
FaeI CATG 2 cut(s) 241, 661
FaiI YATR 8 cut(s) 161, 203, 239, 300, 337, 453, 599, 659
FatI CATG 2 cut(s) 237, 657
FblI GTMKAC 1 cut(s) 71
Fnu4HI GCNGC 1 cut(s) 446
FokI GGATG 1 cut(s) 536
Fsp4HI GCNGC 1 cut(s) 446
FspBI CTAG 1 cut(s) 663
GluI GCNGC 1 cut(s) 446
HaeIII GGCC 3 cut(s) 542, 616, 683
HapII CCGG 5 cut(s) 266, 287, 331, 417, 648
Hin1I GRCGYC 1 cut(s) 74
Hin1II CATG 2 cut(s) 241, 661
HincII GTYRAC 2 cut(s) 72, 392
HindII GTYRAC 2 cut(s) 72, 392
HindIII AAGCTT 1 cut(s) 492
HpaII CCGG 5 cut(s) 266, 287, 331, 417, 648
HphI GGTGA 4 cut(s) 280, 320, 431, 662
Hpy166II GTNNAC 4 cut(s) 6, 72, 392, 706
Hpy188I TCNGA 5 cut(s) 30, 82, 250, 309, 435
Hpy188III TCNNGA 9 cut(s) 47, 155, 173, 193, 238, 370, 506, 727, 735
Hpy8I GTNNAC 4 cut(s) 6, 72, 392, 706
Hpy99I CGWCG 5 cut(s) 70, 73, 76, 110, 309
HpyAV CCTTC 5 cut(s) 107, 265, 392, 532, 795
HpyCH4III ACNGT 3 cut(s) 40, 101, 596
HpyCH4IV ACGT 1 cut(s) 74
HpyF10VI GCNNNNNNNGC 1 cut(s) 644
HpyF3I CTNAG 3 cut(s) 323, 440, 473
HpySE526I ACGT 1 cut(s) 74
Hsp92I GRCGYC 1 cut(s) 74
Hsp92II CATG 2 cut(s) 241, 661
Kzo9I GATC 8 cut(s) 25, 43, 87, 351, 529, 620, 712, 739
LguI GCTCTTC 1 cut(s) 637
LmnI GCTCC 1 cut(s) 551
Lsp1109I GCAGC 1 cut(s) 457
LweI GCATC 1 cut(s) 457
MabI ACCWGGT 1 cut(s) 575
MaeI CTAG 1 cut(s) 663
MaeII ACGT 1 cut(s) 74
MaeIII GTNAC 3 cut(s) 101, 326, 590
MalI GATC 8 cut(s) 27, 45, 89, 353, 531, 622, 714, 741
MboI GATC 8 cut(s) 25, 43, 87, 351, 529, 620, 712, 739
MboII GAAGA 9 cut(s) 69, 129, 188, 311, 399, 402, 422, 654, 750
MflI RGATCY 2 cut(s) 351, 739
MlsI TGGCCA 1 cut(s) 616
MluCI AATT 1 cut(s) 50
MluNI TGGCCA 1 cut(s) 616
MlyI GAGTC 5 cut(s) 143, 397, 398, 430, 451
MmeI TCCRAC 2 cut(s) 287, 458
MnlI CCTC 6 cut(s) 162, 303, 306, 424, 462, 520
Mox20I TGGCCA 1 cut(s) 616
MroXI GAANNNNTTC 1 cut(s) 365
MscI TGGCCA 1 cut(s) 616
MseI TTAA 1 cut(s) 811
Msp20I TGGCCA 1 cut(s) 616
MspI CCGG 5 cut(s) 266, 287, 331, 417, 648
MspR9I CCNGG 2 cut(s) 577, 695
MvaI CCWGG 2 cut(s) 577, 695
MwoI GCNNNNNNNGC 1 cut(s) 644
NdeII GATC 8 cut(s) 25, 43, 87, 351, 529, 620, 712, 739
NlaIII CATG 2 cut(s) 241, 661
NlaIV GGNNCC 1 cut(s) 553
NmeAIII GCCGAG 1 cut(s) 492
NmuCI GTSAC 3 cut(s) 101, 326, 590
PagI TCATGA 1 cut(s) 237
PciSI GCTCTTC 1 cut(s) 637
PcsI WCGNNNNNNNCGW 1 cut(s) 71
PdmI GAANNNNTTC 1 cut(s) 365
PfeI GAWTC 6 cut(s) 83, 167, 292, 316, 425, 568
PfoI TCCNGGA 1 cut(s) 693
PkrI GCNGC 1 cut(s) 447
Ple19I CGATCG 1 cut(s) 90
PleI GAGTC 5 cut(s) 142, 396, 398, 430, 451
PpsI GAGTC 5 cut(s) 142, 396, 398, 430, 451
Psp6I CCWGG 2 cut(s) 575, 693
PspGI CCWGG 2 cut(s) 575, 693
PspN4I GGNNCC 1 cut(s) 553
PspPI GGNCC 1 cut(s) 149
PsuI RGATCY 2 cut(s) 351, 739
PvuI CGATCG 1 cut(s) 90
SalI GTCGAC 1 cut(s) 70
SapI GCTCTTC 1 cut(s) 637
SaqAI TTAA 1 cut(s) 811
SatI GCNGC 1 cut(s) 446
Sau3AI GATC 8 cut(s) 25, 43, 87, 351, 529, 620, 712, 739
Sau96I GGNCC 1 cut(s) 149
SchI GAGTC 5 cut(s) 143, 397, 398, 430, 451
ScrFI CCNGG 2 cut(s) 577, 695
SetI ASST 9 cut(s) 77, 118, 202, 245, 496, 578, 587, 678, 711
SexAI ACCWGGT 1 cut(s) 575
SfaNI GCATC 1 cut(s) 457
SgrDI CGTCGACG 1 cut(s) 70
SinI GGWCC 1 cut(s) 149
Sse9I AATT 1 cut(s) 50
SspMI CTAG 1 cut(s) 663
StyD4I CCNGG 2 cut(s) 575, 693
TaaI ACNGT 3 cut(s) 40, 101, 596
TaiI ACGT 1 cut(s) 77
TaqI TCGA 3 cut(s) 71, 86, 734
TasI AATT 1 cut(s) 50
TfiI GAWTC 6 cut(s) 83, 167, 292, 316, 425, 568
Tru1I TTAA 1 cut(s) 811
Tru9I TTAA 1 cut(s) 811
TscAI CASTG 1 cut(s) 106
TseFI GTSAC 3 cut(s) 101, 326, 590
TseI GCWGC 1 cut(s) 445
Tsp45I GTSAC 3 cut(s) 101, 326, 590
TspDTI ATGAA 3 cut(s) 126, 226, 246
TspGWI ACGGA 1 cut(s) 536
TspRI CASTG 1 cut(s) 106
VpaK11BI GGWCC 1 cut(s) 149
XapI RAATTY 1 cut(s) 50
XmiI GTMKAC 1 cut(s) 71
XmnI GAANNNNTTC 1 cut(s) 365
XspI CTAG 1 cut(s) 663
ZraI GACGTC 1 cut(s) 75
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.