pycom15g19310

RNA binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0000728
Physical Location & Seq
Reverse (-)
13739307 .. 13741492
2186 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g19310.2

Sequence Viewer

Length: 771 bp
ATGTCAATCGCTCTACAGAGAAACGGCGGTGGCGGCCATAATATGAACCGACGGCCGAGGTTCATCCATGGGGCGCCTTGCATCCCCATCTACAACTCGTCCGAGGATAAAGGGTTTGCTCAAGATCGTCGTCTTGACCAGGAGGACTCTTGCAGCAGCTCGGCGTCTTCAGTTGGAAGGAACAGCGACTCGTCTGATGGGTCGTCGGAGGGGGAGGAATCCGGTGAGACTGAGGTGCAGAGCTCGTTTAAAGGACCGTTGGACACCATGGACCAGCTCGAGGAGGTTTTGCCGGTCAAGAGAGGTATATCCAAGTTTTATAGTGGTAAATCAAAGTCCTTTACAAGCCTAGCAGATGTCTCGTCTGTTTCTTCTGTCAAGGAACTTGCAAAGCCAAAAAATCGTTATACAAAGAAGCGCAAGAATTTATTAGCCCAGAATTATTTTCAGGATAAAAACTGCAATGACTCTATGACGAATAATGGTGTTGTGATATCAAAGAGGCCAGCTGCTAACCCTAGTCGAGGATCGTTTATTATTGGGGAGACTTTGAGCAGCTCTCCTAGCTATAGCAATGGGGAGGGATCCAACTCCATTTCACCATCACCTTCTTCCGGTCTTCCACCTCTGCCTCCACACGGTAGACAATCACCTGATAATGAAGCATCGCCACCTCTTCCTCAACGGAGTTCTCCTTGGCGATCATTCTCTCTTTCTGATCTGCAGTGCATTGCTGCAGCAACACCTGACATAACTGGCATGCGAGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

257

Amino Acids

27.45

Weight (kDa)

8.64

Isoelectric Point (pI)

68.44

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 73
AccI GTMKAC 1 cut(s) 643
AciI CCGC 2 cut(s) 27, 33
AclWI GGATC 3 cut(s) 535, 579, 592
AcoI YGGCCR 2 cut(s) 34, 53
AcsI RAATTY 1 cut(s) 424
AcuI CTGAAG 1 cut(s) 153
AcyI GRCGYC 2 cut(s) 74, 164
AfiI CCNNNNNNNGG 4 cut(s) 280, 524, 614, 638
AjnI CCWGG 1 cut(s) 138
AluBI AGCT 6 cut(s) 159, 243, 277, 509, 558, 567
AluI AGCT 6 cut(s) 159, 243, 277, 509, 558, 567
Alw21I GWGCWC 1 cut(s) 245
Alw26I GTCTC 3 cut(s) 221, 364, 539
AlwI GGATC 3 cut(s) 535, 579, 592
Ama87I CYCGRG 1 cut(s) 278
AoxI GGCC 3 cut(s) 34, 53, 503
ApeKI GCWGC 6 cut(s) 153, 156, 509, 555, 734, 737
ApoI RAATTY 1 cut(s) 424
AspLEI GCGC 2 cut(s) 76, 420
AspS9I GGNCC 2 cut(s) 254, 271
AsuHPI GGTGA 4 cut(s) 236, 591, 597, 642
AvaI CYCGRG 1 cut(s) 278
AvaII GGWCC 2 cut(s) 254, 271
BamHI GGATCC 1 cut(s) 584
BanI GGYRCC 1 cut(s) 73
BanII GRGCYC 1 cut(s) 245
BbsI GAAGAC 2 cut(s) 159, 611
Bbv12I GWGCWC 1 cut(s) 245
BbvI GCAGC 6 cut(s) 165, 168, 496, 567, 721, 749
BccI CCATC 3 cut(s) 95, 191, 610
BceAI ACGGC 2 cut(s) 40, 68
BcgI CGANNNNNNTGC 2 cut(s) 342, 376
BciT130I CCWGG 1 cut(s) 140
BcoDI GTCTC 3 cut(s) 221, 364, 539
BfaI CTAG 3 cut(s) 350, 519, 564
BfmI CTRYAG 4 cut(s) 14, 568, 722, 735
BfoI RGCGCY 1 cut(s) 77
BisI GCNGC 7 cut(s) 34, 154, 157, 510, 556, 735, 738
BlsI GCNGC 7 cut(s) 35, 155, 158, 511, 557, 736, 739
Bme1390I CCNGG 1 cut(s) 140
Bme18I GGWCC 2 cut(s) 254, 271
BmeT110I CYCGRG 1 cut(s) 278
BmgT120I GGNCC 2 cut(s) 254, 271
BmiI GGNNCC 2 cut(s) 75, 586
BmrFI CCNGG 1 cut(s) 140
BmsI GCATC 2 cut(s) 90, 674
BpiI GAAGAC 2 cut(s) 159, 611
BplI GAGNNNNNCTC 2 cut(s) 544, 576
BpuEI CTTGAG 1 cut(s) 105
BsaHI GRCGYC 2 cut(s) 74, 164
BsaJI CCNNGG 5 cut(s) 56, 67, 102, 267, 695
BsaWI WCCGGW 2 cut(s) 221, 614
Bsc4I CCNNNNNNNGG 4 cut(s) 280, 524, 614, 638
Bse118I RCCGGY 1 cut(s) 292
Bse1I ACTGG 1 cut(s) 760
Bse3DI GCAATG 3 cut(s) 469, 580, 729
BseBI CCWGG 1 cut(s) 140
BseDI CCNNGG 5 cut(s) 56, 67, 102, 267, 695
BseGI GGATG 2 cut(s) 63, 81
BseLI CCNNNNNNNGG 4 cut(s) 280, 524, 614, 638
BseMI GCAATG 3 cut(s) 469, 580, 729
BseMII CTCAG 1 cut(s) 222
BseNI ACTGG 1 cut(s) 760
BseRI GAGGAG 1 cut(s) 296
BseX3I CGGCCG 1 cut(s) 53
BseXI GCAGC 6 cut(s) 165, 168, 496, 567, 721, 749
BsgI GTGCAG 1 cut(s) 257
Bsh1285I CGRYCG 1 cut(s) 56
BshFI GGCC 3 cut(s) 36, 55, 505
BshNI GGYRCC 1 cut(s) 73
BsiEI CGRYCG 1 cut(s) 56
BsiHKAI GWGCWC 1 cut(s) 245
BsiHKCI CYCGRG 1 cut(s) 278
BsiSI CCGG 3 cut(s) 222, 293, 615
BslI CCNNNNNNNGG 4 cut(s) 280, 524, 614, 638
BsmAI GTCTC 3 cut(s) 221, 364, 539
BsnI GGCC 3 cut(s) 36, 55, 505
BsoBI CYCGRG 1 cut(s) 278
Bsp1286I GDGCHC 1 cut(s) 245
Bsp143I GATC 5 cut(s) 124, 527, 584, 701, 718
Bsp19I CCATGG 2 cut(s) 67, 267
BspACI CCGC 2 cut(s) 27, 33
BspANI GGCC 3 cut(s) 36, 55, 505
BspCNI CTCAG 1 cut(s) 223
BspLI GGNNCC 2 cut(s) 75, 586
BspMAI CTGCAG 2 cut(s) 726, 739
BspPI GGATC 3 cut(s) 535, 579, 592
BspT107I GGYRCC 1 cut(s) 73
BsrDI GCAATG 3 cut(s) 469, 580, 729
BsrFI RCCGGY 1 cut(s) 292
BsrI ACTGG 1 cut(s) 760
BssAI RCCGGY 1 cut(s) 292
BssECI CCNNGG 5 cut(s) 56, 67, 102, 267, 695
BssMI GATC 5 cut(s) 124, 527, 584, 701, 718
BssNI GRCGYC 2 cut(s) 74, 164
BssT1I CCWWGG 3 cut(s) 67, 267, 695
Bst2UI CCWGG 1 cut(s) 140
Bst4CI ACNGT 2 cut(s) 258, 641
Bst6I CTCTTC 1 cut(s) 681
BstACI GRCGYC 2 cut(s) 74, 164
BstC8I GCNNGC 2 cut(s) 507, 761
BstDEI CTNAG 1 cut(s) 231
BstDSI CCRYGG 2 cut(s) 67, 267
BstENI CCTNNNNNAGG 1 cut(s) 522
BstF5I GGATG 2 cut(s) 63, 81
BstH2I RGCGCY 1 cut(s) 77
BstHHI GCGC 2 cut(s) 76, 420
BstKTI GATC 5 cut(s) 127, 530, 587, 704, 721
BstMAI GTCTC 3 cut(s) 221, 364, 539
BstMBI GATC 5 cut(s) 124, 527, 584, 701, 718
BstMCI CGRYCG 1 cut(s) 56
BstMWI GCNNNNNNNGC 2 cut(s) 33, 564
BstNI CCWGG 1 cut(s) 140
BstNSI RCATGY 1 cut(s) 763
BstSCI CCNGG 1 cut(s) 138
BstSFI CTRYAG 4 cut(s) 14, 568, 722, 735
BstV1I GCAGC 6 cut(s) 165, 168, 496, 567, 721, 749
BstV2I GAAGAC 2 cut(s) 159, 611
BstX2I RGATCY 1 cut(s) 584
BstYI RGATCY 1 cut(s) 584
BstZI CGGCCG 1 cut(s) 53
BsuRI GGCC 3 cut(s) 36, 55, 505
BtgI CCRYGG 2 cut(s) 67, 267
BtgZI GCGATG 1 cut(s) 651
BtsCI GGATG 2 cut(s) 63, 81
BtsI GCAGTG 1 cut(s) 731
BtsIMutI CAGTG 1 cut(s) 731
Cac8I GCNNGC 2 cut(s) 507, 761
CfoI GCGC 2 cut(s) 76, 420
Cfr10I RCCGGY 1 cut(s) 292
Cfr13I GGNCC 2 cut(s) 254, 271
CseI GACGC 1 cut(s) 153
CviAII CATG 3 cut(s) 68, 268, 760
DdeI CTNAG 1 cut(s) 231
DinI GGCGCC 1 cut(s) 75
DpnI GATC 5 cut(s) 126, 529, 586, 703, 720
DpnII GATC 5 cut(s) 124, 527, 584, 701, 718
DraI TTTAAA 1 cut(s) 250
EaeI YGGCCR 2 cut(s) 34, 53
EagI CGGCCG 1 cut(s) 53
Eam1104I CTCTTC 1 cut(s) 681
EarI CTCTTC 1 cut(s) 681
Ecl136II GAGCTC 1 cut(s) 243
EclXI CGGCCG 1 cut(s) 53
Eco130I CCWWGG 3 cut(s) 67, 267, 695
Eco24I GRGCYC 1 cut(s) 245
Eco32I GATATC 1 cut(s) 495
Eco47I GGWCC 2 cut(s) 254, 271
Eco52I CGGCCG 1 cut(s) 53
Eco53kI GAGCTC 1 cut(s) 243
Eco57I CTGAAG 1 cut(s) 153
Eco88I CYCGRG 1 cut(s) 278
EcoICRI GAGCTC 1 cut(s) 243
EcoNI CCTNNNNNAGG 1 cut(s) 522
EcoRII CCWGG 1 cut(s) 138
EcoRV GATATC 1 cut(s) 495
EcoT14I CCWWGG 3 cut(s) 67, 267, 695
EcoT38I GRGCYC 1 cut(s) 245
EgeI GGCGCC 1 cut(s) 75
EheI GGCGCC 1 cut(s) 75
ErhI CCWWGG 3 cut(s) 67, 267, 695
FaeI CATG 3 cut(s) 71, 271, 763
FatI CATG 3 cut(s) 67, 267, 759
FblI GTMKAC 1 cut(s) 643
Fnu4HI GCNGC 7 cut(s) 34, 154, 157, 510, 556, 735, 738
FokI GGATG 2 cut(s) 50, 68
FriOI GRGCYC 1 cut(s) 245
Fsp4HI GCNGC 7 cut(s) 34, 154, 157, 510, 556, 735, 738
FspBI CTAG 3 cut(s) 350, 519, 564
GlaI GCGC 2 cut(s) 75, 419
GluI GCNGC 7 cut(s) 34, 154, 157, 510, 556, 735, 738
HaeII RGCGCY 1 cut(s) 77
HaeIII GGCC 3 cut(s) 36, 55, 505
HapII CCGG 3 cut(s) 222, 293, 615
HgaI GACGC 1 cut(s) 153
HhaI GCGC 2 cut(s) 76, 420
Hin1I GRCGYC 2 cut(s) 74, 164
Hin1II CATG 3 cut(s) 71, 271, 763
Hin6I GCGC 2 cut(s) 74, 418
HinP1I GCGC 2 cut(s) 74, 418
HinfI GANTC 4 cut(s) 146, 188, 218, 467
HpaII CCGG 3 cut(s) 222, 293, 615
HphI GGTGA 4 cut(s) 236, 591, 597, 642
Hpy166II GTNNAC 1 cut(s) 644
Hpy188I TCNGA 4 cut(s) 103, 196, 208, 718
Hpy188III TCNNGA 4 cut(s) 122, 134, 298, 449
Hpy8I GTNNAC 1 cut(s) 644
Hpy99I CGWCG 3 cut(s) 54, 132, 208
HpyAV CCTTC 2 cut(s) 171, 618
HpyCH4III ACNGT 2 cut(s) 258, 641
HpyCH4V TGCA 8 cut(s) 81, 153, 238, 389, 462, 724, 729, 737
HpyF10VI GCNNNNNNNGC 2 cut(s) 33, 564
HpyF3I CTNAG 1 cut(s) 231
Hsp92I GRCGYC 2 cut(s) 74, 164
Hsp92II CATG 3 cut(s) 71, 271, 763
HspAI GCGC 2 cut(s) 74, 418
KasI GGCGCC 1 cut(s) 73
Kzo9I GATC 5 cut(s) 124, 527, 584, 701, 718
Lsp1109I GCAGC 6 cut(s) 165, 168, 496, 567, 721, 749
LweI GCATC 2 cut(s) 90, 674
MaeI CTAG 3 cut(s) 350, 519, 564
MalI GATC 5 cut(s) 126, 529, 586, 703, 720
MboI GATC 5 cut(s) 124, 527, 584, 701, 718
MboII GAAGA 5 cut(s) 159, 363, 603, 611, 668
MflI RGATCY 1 cut(s) 584
MhlI GDGCHC 1 cut(s) 245
MluCI AATT 2 cut(s) 424, 439
Mly113I GGCGCC 1 cut(s) 74
MlyI GAGTC 3 cut(s) 140, 182, 461
MmeI TCCRAC 4 cut(s) 154, 186, 240, 612
MseI TTAA 1 cut(s) 249
MspA1I CMGCKG 1 cut(s) 509
MspI CCGG 3 cut(s) 222, 293, 615
MspR9I CCNGG 1 cut(s) 140
MvaI CCWGG 1 cut(s) 140
MwoI GCNNNNNNNGC 2 cut(s) 33, 564
NarI GGCGCC 1 cut(s) 74
NcoI CCATGG 2 cut(s) 67, 267
NdeII GATC 5 cut(s) 124, 527, 584, 701, 718
NlaIII CATG 3 cut(s) 71, 271, 763
NlaIV GGNNCC 2 cut(s) 75, 586
NmeAIII GCCGAG 2 cut(s) 81, 140
NspI RCATGY 1 cut(s) 763
PaeI GCATGC 1 cut(s) 763
PaeR7I CTCGAG 1 cut(s) 278
PfeI GAWTC 1 cut(s) 218
PkrI GCNGC 7 cut(s) 35, 155, 158, 511, 557, 736, 739
PleI GAGTC 3 cut(s) 140, 182, 461
PluTI GGCGCC 1 cut(s) 77
PpsI GAGTC 3 cut(s) 140, 182, 461
Psp124BI GAGCTC 1 cut(s) 245
Psp6I CCWGG 1 cut(s) 138
PspGI CCWGG 1 cut(s) 138
PspN4I GGNNCC 2 cut(s) 75, 586
PspPI GGNCC 2 cut(s) 254, 271
PspXI VCTCGAGB 1 cut(s) 278
PstI CTGCAG 2 cut(s) 726, 739
PsuI RGATCY 1 cut(s) 584
PvuII CAGCTG 1 cut(s) 509
SacI GAGCTC 1 cut(s) 245
SaqAI TTAA 1 cut(s) 249
SatI GCNGC 7 cut(s) 34, 154, 157, 510, 556, 735, 738
Sau3AI GATC 5 cut(s) 124, 527, 584, 701, 718
Sau96I GGNCC 2 cut(s) 254, 271
SchI GAGTC 3 cut(s) 140, 182, 461
ScrFI CCNGG 1 cut(s) 140
SduI GDGCHC 1 cut(s) 245
SfaNI GCATC 2 cut(s) 90, 674
SfcI CTRYAG 4 cut(s) 14, 568, 722, 735
SfoI GGCGCC 1 cut(s) 75
Sfr274I CTCGAG 1 cut(s) 278
SinI GGWCC 2 cut(s) 254, 271
SlaI CTCGAG 1 cut(s) 278
SmlI CTYRAG 2 cut(s) 120, 278
SmoI CTYRAG 2 cut(s) 120, 278
SphI GCATGC 1 cut(s) 763
Sse9I AATT 2 cut(s) 424, 439
SsiI CCGC 2 cut(s) 27, 33
SspDI GGCGCC 1 cut(s) 73
SspMI CTAG 3 cut(s) 350, 519, 564
SstI GAGCTC 1 cut(s) 245
StyD4I CCNGG 1 cut(s) 138
StyI CCWWGG 3 cut(s) 67, 267, 695
TaaI ACNGT 2 cut(s) 258, 641
TaqI TCGA 2 cut(s) 279, 523
TasI AATT 2 cut(s) 424, 439
TauI GCSGC 1 cut(s) 36
TfiI GAWTC 1 cut(s) 218
Tru1I TTAA 1 cut(s) 249
Tru9I TTAA 1 cut(s) 249
TscAI CASTG 1 cut(s) 731
TseI GCWGC 6 cut(s) 153, 156, 509, 555, 734, 737
TspDTI ATGAA 3 cut(s) 52, 59, 675
TspGWI ACGGA 1 cut(s) 700
TspRI CASTG 1 cut(s) 731
VpaK11BI GGWCC 2 cut(s) 254, 271
XagI CCTNNNNNAGG 1 cut(s) 522
XapI RAATTY 1 cut(s) 424
XceI RCATGY 1 cut(s) 763
XhoI CTCGAG 1 cut(s) 278
XmiI GTMKAC 1 cut(s) 643
XspI CTAG 3 cut(s) 350, 519, 564
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.