pycom02g07280

RNA binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0000728
Physical Location & Seq
Reverse (-)
5079062 .. 5081165
2104 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g07280.2

Sequence Viewer

Length: 747 bp
ATGTCAATCGCTCTACGGAGAAACGGCGGTGGCGGCAATGATATGATCCGACGGCCGAGGTTCATTCACGGGGCGCCTTGCATCCCCATCCACGACTCGTCGGAAAATAAAGGGTTTGCTCAAGATCGTCGTCATGACCAGGAGGACTCTTGCAGCAGCTCGTCGTCTTCAGTTGGAAGGAACAGCGACTCGTCCGATGGGTCGTCGGAGGGGGAGGAATCCGGTGAGGCTGAGGTGCAGAGCTCGCTTAAAGGACCGTTGGACACCATGGACCAGCTCGAGGAGGTTTTGCCTGTCAAGAGAGGTATATCCAAGTTTTATAGCGGTAAATCAAAGTCCTTCACAAGCCTAGCAGACATCTCGTCTGTTTCTTCTGTCAAGGAGCTTGCAAAGCCAAAAAATCGTTATACCAAGAAACGCAAGAATGTATTAGCCCAGAATTATTTTCAGGATAAAAACTGCAATGACTTATCAAAGAGACCAGCTGCTAACTCTAGTCGAGCATCGTTTCTTATTGGGGAGACTTTGAGCCGCTCCCCTAGCTATAGCAACGGGGAGGGATCCAACTCAATTTCACCATCACCTTCTTCCTGTCTTCCACCTCTGCCACCACATGGTAGACGATCACCTGATAAAGAACCATCACCACCTCTTCCTCGACGCAGTTCTCCTTGGAGATCATTCTCTCTTTCTGATCTGCAGTGCATCGCTGCAGAAACACCTGACATAACTGGCATGGGAGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

249

Amino Acids

26.78

Weight (kDa)

8.96

Isoelectric Point (pI)

82.78

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 73
AccB7I CCANNNNNTGG 1 cut(s) 614
AccBSI CCGCTC 1 cut(s) 534
AccI GTMKAC 1 cut(s) 619
AciI CCGC 4 cut(s) 27, 33, 324, 532
AclWI GGATC 3 cut(s) 40, 555, 568
AcoI YGGCCR 1 cut(s) 53
AcuI CTGAAG 1 cut(s) 153
AcyI GRCGYC 1 cut(s) 74
AfiI CCNNNNNNNGG 2 cut(s) 280, 614
AhdI GACNNNNNGTC 1 cut(s) 361
AjnI CCWGG 1 cut(s) 138
AluBI AGCT 6 cut(s) 159, 243, 277, 385, 485, 543
AluI AGCT 6 cut(s) 159, 243, 277, 385, 485, 543
Alw21I GWGCWC 1 cut(s) 245
Alw26I GTCTC 2 cut(s) 472, 515
AlwI GGATC 3 cut(s) 40, 555, 568
Ama87I CYCGRG 1 cut(s) 278
AoxI GGCC 1 cut(s) 53
ApeKI GCWGC 4 cut(s) 153, 156, 485, 710
AspLEI GCGC 1 cut(s) 76
AspS9I GGNCC 2 cut(s) 254, 271
AsuHPI GGTGA 5 cut(s) 236, 567, 573, 618, 636
AvaI CYCGRG 1 cut(s) 278
AvaII GGWCC 2 cut(s) 254, 271
BamHI GGATCC 1 cut(s) 560
BanI GGYRCC 1 cut(s) 73
BanII GRGCYC 1 cut(s) 245
BbsI GAAGAC 2 cut(s) 159, 587
Bbv12I GWGCWC 1 cut(s) 245
BbvCI CCTCAGC 1 cut(s) 231
BbvI GCAGC 4 cut(s) 165, 168, 472, 697
BccI CCATC 4 cut(s) 95, 191, 586, 649
BceAI ACGGC 2 cut(s) 40, 68
BcgI CGANNNNNNTGC 2 cut(s) 342, 376
BciT130I CCWGG 1 cut(s) 140
BcoDI GTCTC 2 cut(s) 472, 515
BfaI CTAG 3 cut(s) 350, 495, 540
BfmI CTRYAG 3 cut(s) 544, 698, 711
BfoI RGCGCY 1 cut(s) 77
BisI GCNGC 6 cut(s) 34, 154, 157, 486, 532, 711
BlsI GCNGC 6 cut(s) 35, 155, 158, 487, 533, 712
Bme1390I CCNGG 1 cut(s) 140
Bme18I GGWCC 2 cut(s) 254, 271
BmeRI GACNNNNNGTC 1 cut(s) 361
BmeT110I CYCGRG 1 cut(s) 278
BmgT120I GGNCC 2 cut(s) 254, 271
BmiI GGNNCC 2 cut(s) 75, 562
BmrFI CCNGG 1 cut(s) 140
BmsI GCATC 3 cut(s) 90, 512, 714
BpiI GAAGAC 2 cut(s) 159, 587
Bpu10I CCTNAGC 1 cut(s) 231
BpuEI CTTGAG 1 cut(s) 105
BsaHI GRCGYC 1 cut(s) 74
BsaI GGTCTC 1 cut(s) 472
BsaJI CCNNGG 3 cut(s) 56, 267, 671
BsaWI WCCGGW 1 cut(s) 221
Bsc4I CCNNNNNNNGG 2 cut(s) 280, 614
Bse1I ACTGG 1 cut(s) 736
Bse3DI GCAATG 2 cut(s) 43, 469
BseBI CCWGG 1 cut(s) 140
BseDI CCNNGG 3 cut(s) 56, 267, 671
BseGI GGATG 2 cut(s) 81, 87
BseLI CCNNNNNNNGG 2 cut(s) 280, 614
BseMI GCAATG 2 cut(s) 43, 469
BseMII CTCAG 1 cut(s) 222
BseNI ACTGG 1 cut(s) 736
BseRI GAGGAG 1 cut(s) 296
BseX3I CGGCCG 1 cut(s) 53
BseXI GCAGC 4 cut(s) 165, 168, 472, 697
BsgI GTGCAG 1 cut(s) 257
Bsh1285I CGRYCG 1 cut(s) 56
BshFI GGCC 1 cut(s) 55
BshNI GGYRCC 1 cut(s) 73
BsiEI CGRYCG 1 cut(s) 56
BsiHKAI GWGCWC 1 cut(s) 245
BsiHKCI CYCGRG 1 cut(s) 278
BsiSI CCGG 1 cut(s) 222
BslI CCNNNNNNNGG 2 cut(s) 280, 614
BsmAI GTCTC 2 cut(s) 472, 515
BsnI GGCC 1 cut(s) 55
Bso31I GGTCTC 1 cut(s) 472
BsoBI CYCGRG 1 cut(s) 278
Bsp1286I GDGCHC 1 cut(s) 245
Bsp143I GATC 6 cut(s) 45, 124, 560, 623, 677, 694
Bsp19I CCATGG 1 cut(s) 267
BspACI CCGC 4 cut(s) 27, 33, 324, 532
BspANI GGCC 1 cut(s) 55
BspCNI CTCAG 1 cut(s) 223
BspHI TCATGA 1 cut(s) 133
BspLI GGNNCC 2 cut(s) 75, 562
BspMAI CTGCAG 2 cut(s) 702, 715
BspPI GGATC 3 cut(s) 40, 555, 568
BspT107I GGYRCC 1 cut(s) 73
BspTNI GGTCTC 1 cut(s) 472
BsrBI CCGCTC 1 cut(s) 534
BsrDI GCAATG 2 cut(s) 43, 469
BsrI ACTGG 1 cut(s) 736
BssECI CCNNGG 3 cut(s) 56, 267, 671
BssMI GATC 6 cut(s) 45, 124, 560, 623, 677, 694
BssNI GRCGYC 1 cut(s) 74
BssT1I CCWWGG 2 cut(s) 267, 671
Bst2UI CCWGG 1 cut(s) 140
Bst4CI ACNGT 1 cut(s) 258
Bst6I CTCTTC 1 cut(s) 657
BstACI GRCGYC 1 cut(s) 74
BstC8I GCNNGC 2 cut(s) 245, 387
BstDEI CTNAG 1 cut(s) 231
BstDSI CCRYGG 1 cut(s) 267
BstF5I GGATG 2 cut(s) 81, 87
BstH2I RGCGCY 1 cut(s) 77
BstHHI GCGC 1 cut(s) 76
BstKTI GATC 6 cut(s) 48, 127, 563, 626, 680, 697
BstMAI GTCTC 2 cut(s) 472, 515
BstMBI GATC 6 cut(s) 45, 124, 560, 623, 677, 694
BstMCI CGRYCG 1 cut(s) 56
BstMWI GCNNNNNNNGC 4 cut(s) 33, 244, 391, 540
BstNI CCWGG 1 cut(s) 140
BstSCI CCNGG 1 cut(s) 138
BstSFI CTRYAG 3 cut(s) 544, 698, 711
BstV1I GCAGC 4 cut(s) 165, 168, 472, 697
BstV2I GAAGAC 2 cut(s) 159, 587
BstX2I RGATCY 1 cut(s) 560
BstYI RGATCY 1 cut(s) 560
BstZI CGGCCG 1 cut(s) 53
BsuRI GGCC 1 cut(s) 55
BtgI CCRYGG 1 cut(s) 267
BtgZI GCGATG 1 cut(s) 691
BtsCI GGATG 2 cut(s) 81, 87
BtsI GCAGTG 1 cut(s) 707
BtsIMutI CAGTG 1 cut(s) 707
Cac8I GCNNGC 2 cut(s) 245, 387
CciI TCATGA 1 cut(s) 133
CfoI GCGC 1 cut(s) 76
Cfr13I GGNCC 2 cut(s) 254, 271
CseI GACGC 1 cut(s) 669
CviAII CATG 4 cut(s) 134, 268, 614, 736
DdeI CTNAG 1 cut(s) 231
DinI GGCGCC 1 cut(s) 75
DpnI GATC 6 cut(s) 47, 126, 562, 625, 679, 696
DpnII GATC 6 cut(s) 45, 124, 560, 623, 677, 694
DriI GACNNNNNGTC 1 cut(s) 361
EaeI YGGCCR 1 cut(s) 53
EagI CGGCCG 1 cut(s) 53
Eam1104I CTCTTC 1 cut(s) 657
Eam1105I GACNNNNNGTC 1 cut(s) 361
EarI CTCTTC 1 cut(s) 657
Ecl136II GAGCTC 1 cut(s) 243
EclXI CGGCCG 1 cut(s) 53
Eco130I CCWWGG 2 cut(s) 267, 671
Eco24I GRGCYC 1 cut(s) 245
Eco31I GGTCTC 1 cut(s) 472
Eco47I GGWCC 2 cut(s) 254, 271
Eco52I CGGCCG 1 cut(s) 53
Eco53kI GAGCTC 1 cut(s) 243
Eco57I CTGAAG 1 cut(s) 153
Eco88I CYCGRG 1 cut(s) 278
EcoICRI GAGCTC 1 cut(s) 243
EcoRII CCWGG 1 cut(s) 138
EcoT14I CCWWGG 2 cut(s) 267, 671
EcoT38I GRGCYC 1 cut(s) 245
EgeI GGCGCC 1 cut(s) 75
EheI GGCGCC 1 cut(s) 75
ErhI CCWWGG 2 cut(s) 267, 671
FaeI CATG 4 cut(s) 137, 271, 617, 739
FatI CATG 4 cut(s) 133, 267, 613, 735
FblI GTMKAC 1 cut(s) 619
Fnu4HI GCNGC 6 cut(s) 34, 154, 157, 486, 532, 711
FokI GGATG 2 cut(s) 68, 74
FriOI GRGCYC 1 cut(s) 245
Fsp4HI GCNGC 6 cut(s) 34, 154, 157, 486, 532, 711
FspBI CTAG 3 cut(s) 350, 495, 540
GlaI GCGC 1 cut(s) 75
GluI GCNGC 6 cut(s) 34, 154, 157, 486, 532, 711
HaeII RGCGCY 1 cut(s) 77
HaeIII GGCC 1 cut(s) 55
HapII CCGG 1 cut(s) 222
HgaI GACGC 1 cut(s) 669
HhaI GCGC 1 cut(s) 76
Hin1I GRCGYC 1 cut(s) 74
Hin1II CATG 4 cut(s) 137, 271, 617, 739
Hin6I GCGC 1 cut(s) 74
HinP1I GCGC 1 cut(s) 74
HinfI GANTC 4 cut(s) 95, 146, 188, 218
HpaII CCGG 1 cut(s) 222
HphI GGTGA 5 cut(s) 236, 567, 573, 618, 636
Hpy166II GTNNAC 1 cut(s) 620
Hpy188I TCNGA 5 cut(s) 50, 103, 196, 208, 694
Hpy188III TCNNGA 4 cut(s) 122, 134, 298, 449
Hpy8I GTNNAC 1 cut(s) 620
Hpy99I CGWCG 6 cut(s) 54, 103, 132, 166, 208, 663
HpyAV CCTTC 3 cut(s) 171, 349, 594
HpyCH4III ACNGT 1 cut(s) 258
HpyCH4V TGCA 8 cut(s) 81, 153, 238, 389, 462, 700, 705, 713
HpyF10VI GCNNNNNNNGC 4 cut(s) 33, 244, 391, 540
HpyF3I CTNAG 1 cut(s) 231
Hsp92I GRCGYC 1 cut(s) 74
Hsp92II CATG 4 cut(s) 137, 271, 617, 739
HspAI GCGC 1 cut(s) 74
KasI GGCGCC 1 cut(s) 73
Kzo9I GATC 6 cut(s) 45, 124, 560, 623, 677, 694
LmnI GCTCC 2 cut(s) 382, 539
Lsp1109I GCAGC 4 cut(s) 165, 168, 472, 697
LweI GCATC 3 cut(s) 90, 512, 714
MaeI CTAG 3 cut(s) 350, 495, 540
MalI GATC 6 cut(s) 47, 126, 562, 625, 679, 696
MbiI CCGCTC 1 cut(s) 534
MboI GATC 6 cut(s) 45, 124, 560, 623, 677, 694
MboII GAAGA 5 cut(s) 159, 363, 579, 587, 644
MflI RGATCY 1 cut(s) 560
MhlI GDGCHC 1 cut(s) 245
MluCI AATT 2 cut(s) 439, 570
Mly113I GGCGCC 1 cut(s) 74
MlyI GAGTC 3 cut(s) 89, 140, 182
MmeI TCCRAC 6 cut(s) 73, 81, 154, 186, 240, 588
MseI TTAA 1 cut(s) 249
MspA1I CMGCKG 1 cut(s) 485
MspI CCGG 1 cut(s) 222
MspR9I CCNGG 1 cut(s) 140
MvaI CCWGG 1 cut(s) 140
MwoI GCNNNNNNNGC 4 cut(s) 33, 244, 391, 540
NarI GGCGCC 1 cut(s) 74
NcoI CCATGG 1 cut(s) 267
NdeII GATC 6 cut(s) 45, 124, 560, 623, 677, 694
NlaIII CATG 4 cut(s) 137, 271, 617, 739
NlaIV GGNNCC 2 cut(s) 75, 562
NmeAIII GCCGAG 1 cut(s) 81
PaeR7I CTCGAG 1 cut(s) 278
PagI TCATGA 1 cut(s) 133
PfeI GAWTC 1 cut(s) 218
PflMI CCANNNNNTGG 1 cut(s) 614
PkrI GCNGC 6 cut(s) 35, 155, 158, 487, 533, 712
PleI GAGTC 3 cut(s) 89, 140, 182
PluTI GGCGCC 1 cut(s) 77
PpsI GAGTC 3 cut(s) 89, 140, 182
Psp124BI GAGCTC 1 cut(s) 245
Psp6I CCWGG 1 cut(s) 138
PspGI CCWGG 1 cut(s) 138
PspN4I GGNNCC 2 cut(s) 75, 562
PspPI GGNCC 2 cut(s) 254, 271
PspXI VCTCGAGB 1 cut(s) 278
PstI CTGCAG 2 cut(s) 702, 715
PsuI RGATCY 1 cut(s) 560
PvuII CAGCTG 1 cut(s) 485
SacI GAGCTC 1 cut(s) 245
SaqAI TTAA 1 cut(s) 249
SatI GCNGC 6 cut(s) 34, 154, 157, 486, 532, 711
Sau3AI GATC 6 cut(s) 45, 124, 560, 623, 677, 694
Sau96I GGNCC 2 cut(s) 254, 271
SchI GAGTC 3 cut(s) 89, 140, 182
ScrFI CCNGG 1 cut(s) 140
SduI GDGCHC 1 cut(s) 245
SfaNI GCATC 3 cut(s) 90, 512, 714
SfcI CTRYAG 3 cut(s) 544, 698, 711
SfoI GGCGCC 1 cut(s) 75
Sfr274I CTCGAG 1 cut(s) 278
SinI GGWCC 2 cut(s) 254, 271
SlaI CTCGAG 1 cut(s) 278
SmlI CTYRAG 2 cut(s) 120, 278
SmoI CTYRAG 2 cut(s) 120, 278
Sse9I AATT 2 cut(s) 439, 570
SsiI CCGC 4 cut(s) 27, 33, 324, 532
SspDI GGCGCC 1 cut(s) 73
SspMI CTAG 3 cut(s) 350, 495, 540
SstI GAGCTC 1 cut(s) 245
StyD4I CCNGG 1 cut(s) 138
StyI CCWWGG 2 cut(s) 267, 671
TaaI ACNGT 1 cut(s) 258
TaqI TCGA 3 cut(s) 279, 499, 658
TasI AATT 2 cut(s) 439, 570
TauI GCSGC 2 cut(s) 36, 534
TfiI GAWTC 1 cut(s) 218
Tru1I TTAA 1 cut(s) 249
Tru9I TTAA 1 cut(s) 249
TscAI CASTG 1 cut(s) 707
TseI GCWGC 4 cut(s) 153, 156, 485, 710
TspDTI ATGAA 1 cut(s) 52
TspGWI ACGGA 1 cut(s) 31
TspRI CASTG 1 cut(s) 707
Van91I CCANNNNNTGG 1 cut(s) 614
VpaK11BI GGWCC 2 cut(s) 254, 271
XhoI CTCGAG 1 cut(s) 278
XmiI GTMKAC 1 cut(s) 619
XspI CTAG 3 cut(s) 350, 495, 540
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.