Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0000227

Physical Location & Seq

Genomic Coordinates Forward (+)

16137576 .. 16142270

Gene Structure 4695 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

AT3G44550.2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1491 bp

ATGGAACTCAATTGTGTTCAATTTCTTCGAAACAAGACGATTCTTGTCACCGGAGCTACCGGTTTCCTTGCGAAGGTTTTTGTGGAGAAAATTCTGAGAGTGCAACCAAATGTGAAGAAGCTGTACCTTTTGGTGAGAGCATCTGACAATGAAGCTGCCACTAAACGCTTACGCACAGAGGTATTTGAGAAAGAACTTTTTAAGGTATTGAGACAGAATCTTGGCGATGAGAAATTAAATACATTGTTGTACGAAAAAGTTGTTTCGGTCCCGGGTGATATAGCGACTGATCAATTAGGCATAAATGACTCTCATCTTAGAGAACGTATGCAAAAAGAAATAGATATTGTTGTCAATGTTGCAGCCACAACAAACTTTGACGAAAGATATGATGTTGGGCTTGGGATCAATACATTTGGAGCTCTCAACGTCCTTAACTTTGCCAAAAAATGTGTTAAAGTTCAATTGCTTCTCCATGTCTCAACTGCTTATGTTTGTGGAGAAAAGCCAGGTCTCATACCTGAAAAACCATTCATCATGGAAGAGATTCGTAACGAGAATGGTCTTCAATTGGATATTAACCTTGAAAGGGAGCTTATGAAGCAAAGATTGAAAGAACTCAATGAACAAGATTGTTCCGAAGAAGACATTACTCTTTCCATGAAAGAACTCGGAATGGAAAGGGCCAAGCTTCATGGATGGCCTAACACATATGTTTTCACCAAATCAATGGGAGAAATGCTTCTTGGTAAGCATAAAGAAAATCTCCCCCTCGTCATCATCCGTCCCACGATGATCACTAGTACTCTTTCGGAACCTTTTCCTGGTTGGATCGAAGGCTTGAGAACTGTCGACAGCGTGATTATTGCATATGGAAAGGGAGTGCTCAAGTGTTTTCTTGTCGATGTTAACTCGGTCTGCGATATGATACCAGTGGATATGGTGGCAAACGCAATGATCACGGCTGCAGCCAAACACGCTGGAGGTTCAGGGGTTCACATGGTGTACCATGTCGGCTCATCTCACCAGAACCCAGTCACATTTGGAGAGATTCATGAGATTGCGGTTCGTTACTTTACGAAAAACCCTTTGCGAAGTCGCAATGGCTCACTCATAACCGTCTCAAAAGTGAGGTTCATACCAACCATGGCTTTGTTCAGCCTCTACATGACCCTACGTTACAAACTACCTCTCCAGTTATTAAAACTAGTTGACATAATATATCCTTGGAGAAACGGAGATAAATATGGAGACAAGAACCGCAAAATCGAGTTGGTGATGAGATTGGTAGAGCTTTATGAGCCTTATGTACTCTTCAAGGGAATATTCGACGATAGAAATACAAAGAGTTTATGCGCAAACCAGAAGGAAGAGGAGATCAAAAATACAGAAAAATTGATGTTTGATTTCGACCCAAAAGGAATTAATTGGGGAGATTATCTCACAAATATACACATTTCTGGACTCGTCACACACGTGCTTAAGAAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005886 GO:0006139 GO:0006163 GO:0006637 GO:0006725 GO:0006732 GO:0006753 GO:0006790 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006970 GO:0008150 GO:0008152 GO:0009058 GO:0009117 GO:0009150 GO:0009259 GO:0009507 GO:0009536 GO:0009611 GO:0009628 GO:0009651 GO:0009698 GO:0009699 GO:0009987 GO:0010025 GO:0010166 GO:0010345 GO:0012505 GO:0016020 GO:0016491 GO:0016620 GO:0016903 GO:0019438 GO:0019637 GO:0019693 GO:0019748 GO:0033865 GO:0033875 GO:0034032 GO:0034641 GO:0035336 GO:0035337 GO:0035383 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043603 GO:0044237 GO:0044238 GO:0044249 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0044550 GO:0046483 GO:0050062 GO:0050896 GO:0051186 GO:0055086 GO:0055114 GO:0071704 GO:0071944 GO:0072521 GO:0080019 GO:1901135 GO:1901360 GO:1901362 GO:1901564 GO:1901568 GO:1901570 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00073 ko04146 ko04212 map00073 map04146 map04212

Pfam Domains

Protein Families

NAD_binding_4 Sterile

Protein Analysis

496

Amino Acids

56.43

Weight (kDa)

8.59

Isoelectric Point (pI)

26.51

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
NAD_binding_4	PF07993	2 - 210	9.1e-50	Male sterility protein
Sterile	PF03015	285 - 386	4.1e-15	Male sterility protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000227)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G44550 AT3G44550 AT3G44560 AT3G44560 AT3G44560
fragaria_vesca	FvH4_3g09600 FvH4_3g24560 FvH4_3g24560 FvH4_3g24560 FvH4_3g24570 FvH4_3g24570 FvH4_3g38410 FvH4_3g38410 FvH4_3g38410 FvH4_5g01800 FvH4_7g07480 FvH4_7g07480
malus_domestica	MD02G1239300.v1.1 MD03G1141400.v1.1 MD03G1141600.v1.1 MD03G1141700.v1.1 MD10G1066600.v1.1 MD11G1171600.v1.1
prunus_persica	Prupe.6G126600_v2.0.a1 Prupe.6G126700_v2.0.a1 Prupe.6G126700_v2.0.a1 Prupe.6G126800_v2.0.a1
pyrus_communis	pycom03g09610 pycom03g09620 pycom03g09650 pycom10g05210 pycom11g13330
rosa_chinensis	RchiOBHm_Chr3g0483921 RchiOBHm_Chr5g0043321 RchiOBHm_Chr5g0044031 RchiOBHm_Chr5g0044071 RchiOBHm_Chr5g0069061 RchiOBHm_Chr5g0069111 RchiOBHm_Chr5g0069131 RchiOBHm_Chr6g0256891 RchiOBHm_Chr6g0256901 RchiOBHm_Chr7g0198821 RchiOBHm_Chr7g0198851 RchiOBHm_Chr7g0198861 RchiOBHm_Chr7g0219381 RchiOBHm_Chr7g0230491
rosa_laevigata	RLG00000001491 RLG00000003837 RLG00000014654 RLG00000014655 RLG00000020958 RLG00000023252 RLG00000028966 RLG00000034179 RLG00000034211 RLG00000034236 RLG00000034238 RLG00000036034 RLG00000036038
rosa_multiflora	Rmu_co8020118.1_g000001 Rmu_sc0000060.1_g000002 Rmu_sc0000060.1_g000026 Rmu_sc0001157.1_g000010 Rmu_sc0001157.1_g000016 Rmu_sc0002252.1_g000009 Rmu_sc0003076.1_g000025 Rmu_sc0008623.1_g000018 Rmu_sc0008623.1_g000021 Rmu_sc0022203.1_g000001
rosa_roxburghii	Rroxscaffold_1G00036940 Rroxscaffold_1G00036990 Rroxscaffold_3G00230110 Rroxscaffold_3G00257340 Rroxscaffold_3G00257380 Rroxscaffold_6G00398020 Rroxscaffold_6G00398040 Rroxscaffold_7G00208240 Rroxscaffold_7G00208260
rosa_rugosa	Rorug01G0059500 Rorug03G0208700 Rorug03G0208700 Rorug03G0208800 Rorug03G0208900 Rorug03G0208900 Rorug04G0119300 Rorug05G0210300 Rorug05G0210300 Rorug05G0395800 Rorug05G0396000 Rorug05G0396100 Rorug05G0396200 Rorug05G0577200 Rorug07G0050100 Rorug07G0050200 Rorug07G0215200
rosa_samantha	Rh1BG061200 Rh2BG536700 Rh2DG545600 Rh3AG257500 Rh3BG294300 Rh3BG322600 Rh3CG292100 Rh3DG287300 Rh4BG178300 Rh5AG290800 Rh5AG291000 Rh5AG294200 Rh5AG294300 Rh5BG297400 Rh5BG302600 Rh5BG303000 Rh5CG329900 Rh5CG330100 Rh5DG307000 Rh5DG311500 Rh5DG311700 Rh6AG095800 Rh6BG086800 Rh6BG086900 Rh6CG082900 Rh6DG078600 Rh6DG078700 Rh7AG173700 Rh7AG174000 Rh7AG174100 Rh7AG327500 Rh7AG412100 Rh7BG177100 Rh7CG182900 Rh7CG183100 Rh7CG344800 Rh7CG429900 Rh7DG176600
rosa_wichuraiana	Rw0G003880 Rw0G006090 Rw2G043200 Rw3G023270 Rw3G023310 Rw5G026990 Rw5G027280 Rw5G027300 Rw5G042270 Rw6G008160 Rw7G015210 Rw7G015230

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	1357
AccI	GTMKAC	1 cut(s)	852
AciI	CCGC	2 cut(s)	1064, 1261
AclWI	GGATC	2 cut(s)	413, 839
AcsI	RAATTY	1 cut(s)	90
AcvI	CACGTG	1 cut(s)	1477
AdeI	CACNNNGTG	1 cut(s)	1003
AfaI	GTAC	5 cut(s)	125, 251, 805, 1007, 1311
AfiI	CCNNNNNNNGG	3 cut(s)	73, 589, 824
AflII	CTTAAG	1 cut(s)	1481
AflIII	ACRYGT	1 cut(s)	1474
AgeI	ACCGGT	1 cut(s)	59
AgsI	TTSAA	6 cut(s)	20, 464, 569, 587, 613, 1318
AhlI	ACTAGT	2 cut(s)	800, 1207
AjnI	CCWGG	2 cut(s)	508, 823
AjuI	GAANNNNNNNTTGG	2 cut(s)	729, 761
AleI	CACNNNNGTG	1 cut(s)	1475
AluBI	AGCT	7 cut(s)	56, 121, 155, 422, 595, 691, 1294
AluI	AGCT	7 cut(s)	56, 121, 155, 422, 595, 691, 1294
Alw21I	GWGCWC	2 cut(s)	424, 888
Alw26I	GTCTC	5 cut(s)	205, 484, 518, 1126, 1245
AlwI	GGATC	2 cut(s)	413, 839
Ama87I	CYCGRG	1 cut(s)	271
AoxI	GGCC	2 cut(s)	684, 701
ApeKI	GCWGC	4 cut(s)	155, 362, 965, 968
ApoI	RAATTY	1 cut(s)	90
AseI	ATTAAT	1 cut(s)	1425
AsiGI	ACCGGT	1 cut(s)	59
Asp700I	GAANNNNTTC	3 cut(s)	546, 741, 819
AspLEI	GCGC	1 cut(s)	1358
AspS9I	GGNCC	2 cut(s)	268, 684
AsuC2I	CCSGG	2 cut(s)	272, 273
AsuHPI	GGTGA	6 cut(s)	40, 145, 287, 712, 1016, 1288
AsuII	TTCGAA	1 cut(s)	28
AvaI	CYCGRG	1 cut(s)	271
AvaII	GGWCC	1 cut(s)	268
BanII	GRGCYC	1 cut(s)	424
BarI	GAAGNNNNNNTAC	2 cut(s)	107, 139
BbrPI	CACGTG	1 cut(s)	1477
BbsI	GAAGAC	2 cut(s)	557, 651
Bbv12I	GWGCWC	2 cut(s)	424, 888
BbvI	GCAGC	4 cut(s)	142, 374, 952, 980
BccI	CCATC	1 cut(s)	693
BceAI	ACGGC	1 cut(s)	978
BciT130I	CCWGG	2 cut(s)	510, 825
BclI	TGATCA	3 cut(s)	289, 795, 957
BcnI	CCSGG	2 cut(s)	272, 273
BcoDI	GTCTC	5 cut(s)	205, 484, 518, 1126, 1245
BcuI	ACTAGT	2 cut(s)	800, 1207
BfaI	CTAG	2 cut(s)	801, 1208
BfmI	CTRYAG	1 cut(s)	966
BfrI	CTTAAG	1 cut(s)	1481
BisI	GCNGC	4 cut(s)	156, 363, 966, 969
BlsI	GCNGC	4 cut(s)	157, 364, 967, 970
BmcAI	AGTACT	1 cut(s)	805
Bme1390I	CCNGG	4 cut(s)	272, 273, 510, 825
Bme18I	GGWCC	1 cut(s)	268
BmeT110I	CYCGRG	1 cut(s)	271
BmgT120I	GGNCC	2 cut(s)	268, 684
BmiI	GGNNCC	2 cut(s)	270, 816
BmrFI	CCNGG	4 cut(s)	272, 273, 510, 825
BmrI	ACTGGG	1 cut(s)	1028
BmsI	GCATC	1 cut(s)	149
BmuI	ACTGGG	1 cut(s)	1028
BpiI	GAAGAC	2 cut(s)	557, 651
BplI	GAGNNNNNCTC	2 cut(s)	1425, 1457
BpmI	CTGGAG	2 cut(s)	1002, 1178
Bpu14I	TTCGAA	1 cut(s)	28
BpuEI	CTTGAG	2 cut(s)	862, 872
BpuMI	CCSGG	2 cut(s)	272, 273
BsaAI	YACGTR	1 cut(s)	1477
BsaI	GGTCTC	1 cut(s)	518
BsaJI	CCNNGG	3 cut(s)	271, 1146, 1226
BsaWI	WCCGGW	2 cut(s)	50, 59
BsaXI	ACNNNNNCTCC	2 cut(s)	1176, 1206
Bsc4I	CCNNNNNNNGG	3 cut(s)	73, 589, 824
Bse118I	RCCGGY	1 cut(s)	59
Bse1I	ACTGG	3 cut(s)	932, 1034, 1195
Bse3DI	GCAATG	2 cut(s)	960, 1108
BseBI	CCWGG	2 cut(s)	510, 825
BseDI	CCNNGG	3 cut(s)	271, 1146, 1226
BseGI	GGATG	2 cut(s)	704, 780
BseLI	CCNNNNNNNGG	3 cut(s)	73, 589, 824
BseMI	GCAATG	2 cut(s)	960, 1108
BseMII	CTCAG	1 cut(s)	86
BseNI	ACTGG	3 cut(s)	932, 1034, 1195
BseRI	GAGGAG	1 cut(s)	1388
BseXI	GCAGC	4 cut(s)	142, 374, 952, 980
BshFI	GGCC	2 cut(s)	686, 703
BshTI	ACCGGT	1 cut(s)	59
BsiHKAI	GWGCWC	2 cut(s)	424, 888
BsiHKCI	CYCGRG	1 cut(s)	271
BsiSI	CCGG	3 cut(s)	51, 60, 272
BslFI	GGGAC	2 cut(s)	254, 771
BslI	CCNNNNNNNGG	3 cut(s)	73, 589, 824
BsmAI	GTCTC	5 cut(s)	205, 484, 518, 1126, 1245
BsmBI	CGTCTC	1 cut(s)	1126
BsmFI	GGGAC	2 cut(s)	254, 771
BsnI	GGCC	2 cut(s)	686, 703
Bso31I	GGTCTC	1 cut(s)	518
BsoBI	CYCGRG	1 cut(s)	271
Bsp119I	TTCGAA	1 cut(s)	28
Bsp1286I	GDGCHC	2 cut(s)	424, 888
Bsp143I	GATC	6 cut(s)	289, 405, 795, 831, 957, 1377
Bsp19I	CCATGG	1 cut(s)	1146
BspACI	CCGC	2 cut(s)	1064, 1261
BspANI	GGCC	2 cut(s)	686, 703
BspCNI	CTCAG	1 cut(s)	87
BspHI	TCATGA	1 cut(s)	1054
BspLI	GGNNCC	2 cut(s)	270, 816
BspMAI	CTGCAG	1 cut(s)	970
BspPI	GGATC	2 cut(s)	413, 839
BspT104I	TTCGAA	1 cut(s)	28
BspTI	CTTAAG	1 cut(s)	1481
BspTNI	GGTCTC	1 cut(s)	518
BsrDI	GCAATG	2 cut(s)	960, 1108
BsrFI	RCCGGY	1 cut(s)	59
BsrI	ACTGG	3 cut(s)	932, 1034, 1195
BssAI	RCCGGY	1 cut(s)	59
BssECI	CCNNGG	3 cut(s)	271, 1146, 1226
BssMI	GATC	6 cut(s)	289, 405, 795, 831, 957, 1377
BssT1I	CCWWGG	2 cut(s)	1146, 1226
Bst2UI	CCWGG	2 cut(s)	510, 825
Bst4CI	ACNGT	2 cut(s)	850, 1120
Bst6I	CTCTTC	3 cut(s)	537, 1319, 1365
BstAFI	CTTAAG	1 cut(s)	1481
BstBAI	YACGTR	1 cut(s)	1477
BstBI	TTCGAA	1 cut(s)	28
BstDEI	CTNAG	2 cut(s)	95, 317
BstDSI	CCRYGG	1 cut(s)	1146
BstENI	CCTNNNNNAGG	1 cut(s)	71
BstF5I	GGATG	2 cut(s)	704, 780
BstHHI	GCGC	1 cut(s)	1358
BstKTI	GATC	6 cut(s)	292, 408, 798, 834, 960, 1380
BstMAI	GTCTC	5 cut(s)	205, 484, 518, 1126, 1245
BstMBI	GATC	6 cut(s)	289, 405, 795, 831, 957, 1377
BstMWI	GCNNNNNNNGC	3 cut(s)	601, 977, 1300
BstNI	CCWGG	2 cut(s)	510, 825
BstSCI	CCNGG	4 cut(s)	270, 271, 508, 823
BstSFI	CTRYAG	1 cut(s)	966
BstV1I	GCAGC	4 cut(s)	142, 374, 952, 980
BstV2I	GAAGAC	2 cut(s)	557, 651
BstXI	CCANNNNNNTGG	1 cut(s)	730
BsuRI	GGCC	2 cut(s)	686, 703
BtgI	CCRYGG	1 cut(s)	1146
BtgZI	GCGATG	1 cut(s)	240
BtsCI	GGATG	2 cut(s)	704, 780
BtsIMutI	CAGTG	1 cut(s)	939
CciI	TCATGA	1 cut(s)	1054
CfoI	GCGC	1 cut(s)	1358
Cfr10I	RCCGGY	1 cut(s)	59
Cfr13I	GGNCC	2 cut(s)	268, 684
Cfr9I	CCCGGG	1 cut(s)	271
Csp6I	GTAC	5 cut(s)	124, 250, 804, 1006, 1310
CspAI	ACCGGT	1 cut(s)	59
CviAII	CATG	9 cut(s)	476, 538, 661, 695, 1000, 1010, 1055, 1147, 1168
CviQI	GTAC	5 cut(s)	124, 250, 804, 1006, 1310
DdeI	CTNAG	2 cut(s)	95, 317
DpnI	GATC	6 cut(s)	291, 407, 797, 833, 959, 1379
DpnII	GATC	6 cut(s)	289, 405, 795, 831, 957, 1377
DraIII	CACNNNGTG	1 cut(s)	1003
Eam1104I	CTCTTC	3 cut(s)	537, 1319, 1365
EarI	CTCTTC	3 cut(s)	537, 1319, 1365
Ecl136II	GAGCTC	1 cut(s)	422
Eco130I	CCWWGG	2 cut(s)	1146, 1226
Eco24I	GRGCYC	1 cut(s)	424
Eco31I	GGTCTC	1 cut(s)	518
Eco47I	GGWCC	1 cut(s)	268
Eco53kI	GAGCTC	1 cut(s)	422
Eco72I	CACGTG	1 cut(s)	1477
Eco88I	CYCGRG	1 cut(s)	271
EcoICRI	GAGCTC	1 cut(s)	422
EcoNI	CCTNNNNNAGG	1 cut(s)	71
EcoRII	CCWGG	2 cut(s)	508, 823
EcoT14I	CCWWGG	2 cut(s)	1146, 1226
EcoT38I	GRGCYC	1 cut(s)	424
ErhI	CCWWGG	2 cut(s)	1146, 1226
Esp3I	CGTCTC	1 cut(s)	1126
FaeI	CATG	9 cut(s)	479, 541, 664, 698, 1003, 1013, 1058, 1150, 1171
FaqI	GGGAC	2 cut(s)	254, 771
FatI	CATG	9 cut(s)	475, 537, 660, 694, 999, 1009, 1054, 1146, 1167
FauNDI	CATATG	2 cut(s)	712, 871
FbaI	TGATCA	3 cut(s)	289, 795, 957
FblI	GTMKAC	1 cut(s)	852
Fnu4HI	GCNGC	4 cut(s)	156, 363, 966, 969
FokI	GGATG	2 cut(s)	711, 767
FriOI	GRGCYC	1 cut(s)	424
Fsp4HI	GCNGC	4 cut(s)	156, 363, 966, 969
FspBI	CTAG	2 cut(s)	801, 1208
FspI	TGCGCA	1 cut(s)	1357
GlaI	GCGC	1 cut(s)	1357
GluI	GCNGC	4 cut(s)	156, 363, 966, 969
GsuI	CTGGAG	2 cut(s)	1002, 1178
HaeIII	GGCC	2 cut(s)	686, 703
HapII	CCGG	3 cut(s)	51, 60, 272
HhaI	GCGC	1 cut(s)	1358
Hin1II	CATG	9 cut(s)	479, 541, 664, 698, 1003, 1013, 1058, 1150, 1171
Hin6I	GCGC	1 cut(s)	1356
HinP1I	GCGC	1 cut(s)	1356
HincII	GTYRAC	3 cut(s)	853, 910, 1213
HindII	GTYRAC	3 cut(s)	853, 910, 1213
HindIII	AAGCTT	1 cut(s)	689
HinfI	GANTC	6 cut(s)	40, 217, 308, 547, 1051, 1464
HpaI	GTTAAC	1 cut(s)	910
HpaII	CCGG	3 cut(s)	51, 60, 272
HphI	GGTGA	6 cut(s)	40, 145, 287, 712, 1016, 1288
Hpy166II	GTNNAC	5 cut(s)	853, 910, 997, 1006, 1213
Hpy188I	TCNGA	5 cut(s)	96, 145, 640, 674, 814
Hpy188III	TCNNGA	2 cut(s)	1055, 1461
Hpy8I	GTNNAC	5 cut(s)	853, 910, 997, 1006, 1213
Hpy99I	CGWCG	1 cut(s)	1334
HpyAV	CCTTC	3 cut(s)	67, 830, 1360
HpyCH4III	ACNGT	2 cut(s)	850, 1120
HpyCH4IV	ACGT	4 cut(s)	325, 429, 1177, 1476
HpyCH4V	TGCA	5 cut(s)	103, 331, 362, 869, 968
HpyF10VI	GCNNNNNNNGC	3 cut(s)	601, 977, 1300
HpyF3I	CTNAG	2 cut(s)	95, 317
HpySE526I	ACGT	4 cut(s)	325, 429, 1177, 1476
Hsp92II	CATG	9 cut(s)	479, 541, 664, 698, 1003, 1013, 1058, 1150, 1171
HspAI	GCGC	1 cut(s)	1356
Ksp22I	TGATCA	3 cut(s)	289, 795, 957
KspAI	GTTAAC	1 cut(s)	910
Kzo9I	GATC	6 cut(s)	289, 405, 795, 831, 957, 1377
LmnI	GCTCC	3 cut(s)	53, 419, 592
Lsp1109I	GCAGC	4 cut(s)	142, 374, 952, 980
LweI	GCATC	1 cut(s)	149
MaeI	CTAG	2 cut(s)	801, 1208
MaeII	ACGT	4 cut(s)	325, 429, 1177, 1476
MaeIII	GTNAC	6 cut(s)	46, 551, 1036, 1070, 1178, 1468
MalI	GATC	6 cut(s)	291, 407, 797, 833, 959, 1379
MboI	GATC	6 cut(s)	289, 405, 795, 831, 957, 1377
MboII	GAAGA	8 cut(s)	17, 127, 554, 557, 653, 656, 1306, 1382
MfeI	CAATTG	3 cut(s)	10, 464, 569
MhlI	GDGCHC	2 cut(s)	424, 888
MlyI	GAGTC	2 cut(s)	302, 1458
MmeI	TCCRAC	1 cut(s)	809
MnlI	CCTC	7 cut(s)	172, 782, 977, 1125, 1172, 1200, 1366
MroXI	GAANNNNTTC	3 cut(s)	546, 741, 819
MseI	TTAA	9 cut(s)	201, 236, 435, 456, 579, 909, 1202, 1425, 1482
MslI	CAYNNNNRTG	1 cut(s)	1475
MspCI	CTTAAG	1 cut(s)	1481
MspI	CCGG	3 cut(s)	51, 60, 272
MspR9I	CCNGG	4 cut(s)	272, 273, 510, 825
MunI	CAATTG	3 cut(s)	10, 464, 569
MvaI	CCWGG	2 cut(s)	510, 825
MwoI	GCNNNNNNNGC	3 cut(s)	601, 977, 1300
NciI	CCSGG	2 cut(s)	272, 273
NcoI	CCATGG	1 cut(s)	1146
NdeI	CATATG	2 cut(s)	712, 871
NdeII	GATC	6 cut(s)	289, 405, 795, 831, 957, 1377
NlaIII	CATG	9 cut(s)	479, 541, 664, 698, 1003, 1013, 1058, 1150, 1171
NlaIV	GGNNCC	2 cut(s)	270, 816
NmuCI	GTSAC	3 cut(s)	46, 1036, 1468
NsbI	TGCGCA	1 cut(s)	1357
NspV	TTCGAA	1 cut(s)	28
OliI	CACNNNNGTG	1 cut(s)	1475
PagI	TCATGA	1 cut(s)	1054
PcsI	WCGNNNNNNNCGW	1 cut(s)	1473
PdmI	GAANNNNTTC	3 cut(s)	546, 741, 819
PfeI	GAWTC	4 cut(s)	40, 217, 547, 1051
PinAI	ACCGGT	1 cut(s)	59
PkrI	GCNGC	4 cut(s)	157, 364, 967, 970
PleI	GAGTC	2 cut(s)	302, 1458
PmaCI	CACGTG	1 cut(s)	1477
PmlI	CACGTG	1 cut(s)	1477
PpsI	GAGTC	2 cut(s)	302, 1458
Ppu21I	YACGTR	1 cut(s)	1477
PshBI	ATTAAT	1 cut(s)	1425
Psp124BI	GAGCTC	1 cut(s)	424
Psp6I	CCWGG	2 cut(s)	508, 823
PspCI	CACGTG	1 cut(s)	1477
PspGI	CCWGG	2 cut(s)	508, 823
PspN4I	GGNNCC	2 cut(s)	270, 816
PspPI	GGNCC	2 cut(s)	268, 684
PstI	CTGCAG	1 cut(s)	970
RsaI	GTAC	5 cut(s)	125, 251, 805, 1007, 1311
RsaNI	GTAC	5 cut(s)	124, 250, 804, 1006, 1310
RseI	CAYNNNNRTG	1 cut(s)	1475
SacI	GAGCTC	1 cut(s)	424
SalI	GTCGAC	1 cut(s)	851
SaqAI	TTAA	9 cut(s)	201, 236, 435, 456, 579, 909, 1202, 1425, 1482
SatI	GCNGC	4 cut(s)	156, 363, 966, 969
Sau3AI	GATC	6 cut(s)	289, 405, 795, 831, 957, 1377
Sau96I	GGNCC	2 cut(s)	268, 684
ScaI	AGTACT	1 cut(s)	805
SchI	GAGTC	2 cut(s)	302, 1458
ScrFI	CCNGG	4 cut(s)	272, 273, 510, 825
SduI	GDGCHC	2 cut(s)	424, 888
SfaNI	GCATC	1 cut(s)	149
SfcI	CTRYAG	1 cut(s)	966
SfuI	TTCGAA	1 cut(s)	28
SinI	GGWCC	1 cut(s)	268
SmaI	CCCGGG	1 cut(s)	273
SmiMI	CAYNNNNRTG	1 cut(s)	1475
SmlI	CTYRAG	3 cut(s)	841, 887, 1481
SmoI	CTYRAG	3 cut(s)	841, 887, 1481
SpeI	ACTAGT	2 cut(s)	800, 1207
SsiI	CCGC	2 cut(s)	1064, 1261
SspI	AATATT	1 cut(s)	1326
SspMI	CTAG	2 cut(s)	801, 1208
SstI	GAGCTC	1 cut(s)	424
StyD4I	CCNGG	4 cut(s)	270, 271, 508, 823
StyI	CCWWGG	2 cut(s)	1146, 1226
TaaI	ACNGT	2 cut(s)	850, 1120
TaiI	ACGT	4 cut(s)	328, 432, 1180, 1479
TaqI	TCGA	7 cut(s)	28, 834, 852, 903, 1269, 1329, 1410
TaqII	GACCGA	2 cut(s)	256, 904
TatI	WGTACW	2 cut(s)	803, 1309
TfiI	GAWTC	4 cut(s)	40, 217, 547, 1051
Tru1I	TTAA	9 cut(s)	201, 236, 435, 456, 579, 909, 1202, 1425, 1482
Tru9I	TTAA	9 cut(s)	201, 236, 435, 456, 579, 909, 1202, 1425, 1482
TscAI	CASTG	1 cut(s)	939
TseFI	GTSAC	3 cut(s)	46, 1036, 1468
TseI	GCWGC	4 cut(s)	155, 362, 965, 968
Tsp45I	GTSAC	3 cut(s)	46, 1036, 1468
TspDTI	ATGAA	8 cut(s)	165, 523, 614, 639, 677, 683, 1043, 1126
TspGWI	ACGGA	2 cut(s)	773, 1251
TspMI	CCCGGG	1 cut(s)	271
TspRI	CASTG	1 cut(s)	939
Vha464I	CTTAAG	1 cut(s)	1481
VpaK11BI	GGWCC	1 cut(s)	268
VspI	ATTAAT	1 cut(s)	1425
XagI	CCTNNNNNAGG	1 cut(s)	71
XapI	RAATTY	1 cut(s)	90
XmaI	CCCGGG	1 cut(s)	271
XmiI	GTMKAC	1 cut(s)	852
XmnI	GAANNNNTTC	3 cut(s)	546, 741, 819
XspI	CTAG	2 cut(s)	801, 1208
ZrmI	AGTACT	1 cut(s)	805

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.