Catalyzes the reduction of fatty acyl-CoA to fatty alcohols
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc16I | TGCGCA | 1 cut(s) | 960 |
| AccB1I | GGYRCC | 1 cut(s) | 623 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 329 |
| AccI | GTMKAC | 1 cut(s) | 595 |
| AccII | CGCG | 1 cut(s) | 876 |
| AciI | CCGC | 2 cut(s) | 420, 876 |
| AclWI | GGATC | 3 cut(s) | 91, 785, 935 |
| AcoI | YGGCCR | 1 cut(s) | 279 |
| AcuI | CTGAAG | 2 cut(s) | 78, 877 |
| AcvI | CACGTG | 1 cut(s) | 1048 |
| AfaI | GTAC | 5 cut(s) | 266, 615, 651, 661, 970 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 200, 306, 329, 364 |
| AflIII | ACRYGT | 3 cut(s) | 483, 598, 1045 |
| AgsI | TTSAA | 5 cut(s) | 155, 209, 470, 490, 753 |
| AhlI | ACTAGT | 3 cut(s) | 221, 370, 505 |
| AjnI | CCWGG | 3 cut(s) | 363, 517, 640 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 880, 912 |
| Alw21I | GWGCWC | 1 cut(s) | 216 |
| Alw26I | GTCTC | 5 cut(s) | 474, 713, 783, 854, 1175 |
| AlwI | GGATC | 3 cut(s) | 91, 785, 935 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 863 |
| Ama87I | CYCGRG | 3 cut(s) | 254, 780, 802 |
| AoxI | GGCC | 4 cut(s) | 272, 279, 297, 638 |
| AspLEI | GCGC | 1 cut(s) | 961 |
| AspS9I | GGNCC | 3 cut(s) | 644, 727, 1066 |
| AsuHPI | GGTGA | 2 cut(s) | 7, 1073 |
| AvaI | CYCGRG | 3 cut(s) | 254, 780, 802 |
| AvaII | GGWCC | 3 cut(s) | 644, 727, 1066 |
| BaeGI | GKGCMC | 1 cut(s) | 825 |
| BalI | TGGCCA | 1 cut(s) | 281 |
| BanI | GGYRCC | 1 cut(s) | 623 |
| BanII | GRGCYC | 2 cut(s) | 171, 669 |
| BbrPI | CACGTG | 1 cut(s) | 1048 |
| BbsI | GAAGAC | 1 cut(s) | 1310 |
| Bbv12I | GWGCWC | 1 cut(s) | 216 |
| BccI | CCATC | 3 cut(s) | 395, 593, 1283 |
| BciT130I | CCWGG | 3 cut(s) | 365, 519, 642 |
| BciVI | GTATCC | 2 cut(s) | 201, 778 |
| BcoDI | GTCTC | 5 cut(s) | 474, 713, 783, 854, 1175 |
| BcuI | ACTAGT | 3 cut(s) | 221, 370, 505 |
| BfaI | CTAG | 5 cut(s) | 114, 222, 371, 506, 723 |
| BfmI | CTRYAG | 1 cut(s) | 1266 |
| BfuI | GTATCC | 2 cut(s) | 201, 778 |
| BlpI | GCTNAGC | 1 cut(s) | 240 |
| Bme1390I | CCNGG | 3 cut(s) | 365, 519, 642 |
| Bme18I | GGWCC | 3 cut(s) | 644, 727, 1066 |
| BmeT110I | CYCGRG | 3 cut(s) | 254, 780, 802 |
| BmgT120I | GGNCC | 3 cut(s) | 644, 727, 1066 |
| BmiI | GGNNCC | 3 cut(s) | 625, 728, 729 |
| BmrFI | CCNGG | 3 cut(s) | 365, 519, 642 |
| BmrI | ACTGGG | 1 cut(s) | 672 |
| BmsI | GCATC | 4 cut(s) | 340, 399, 571, 970 |
| BmuI | ACTGGG | 1 cut(s) | 672 |
| BpiI | GAAGAC | 1 cut(s) | 1310 |
| BpmI | CTGGAG | 2 cut(s) | 347, 540 |
| Bpu10I | CCTNAGC | 1 cut(s) | 170 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 240 |
| BpuEI | CTTGAG | 2 cut(s) | 122, 610 |
| Bsa29I | ATCGAT | 1 cut(s) | 579 |
| BsaAI | YACGTR | 1 cut(s) | 1048 |
| BsaBI | GATNNNNATC | 1 cut(s) | 789 |
| BsaJI | CCNNGG | 1 cut(s) | 1107 |
| BsaWI | WCCGGW | 1 cut(s) | 646 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 880, 910 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 200, 306, 329, 364 |
| Bse1I | ACTGG | 4 cut(s) | 292, 323, 625, 667 |
| Bse3DI | GCAATG | 4 cut(s) | 447, 756, 1023, 1300 |
| Bse8I | GATNNNNATC | 1 cut(s) | 789 |
| BseBI | CCWGG | 3 cut(s) | 365, 519, 642 |
| BseCI | ATCGAT | 1 cut(s) | 579 |
| BseDI | CCNNGG | 1 cut(s) | 1107 |
| BseGI | GGATG | 3 cut(s) | 165, 355, 1294 |
| BseJI | GATNNNNATC | 1 cut(s) | 789 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 200, 306, 329, 364 |
| BseMI | GCAATG | 4 cut(s) | 447, 756, 1023, 1300 |
| BseMII | CTCAG | 5 cut(s) | 135, 184, 231, 891, 1128 |
| BseNI | ACTGG | 4 cut(s) | 292, 323, 625, 667 |
| BseRI | GAGGAG | 3 cut(s) | 83, 190, 1247 |
| BseSI | GKGCMC | 1 cut(s) | 825 |
| BseYI | CCCAGC | 1 cut(s) | 199 |
| BsgI | GTGCAG | 1 cut(s) | 1109 |
| Bsh1236I | CGCG | 1 cut(s) | 876 |
| BshFI | GGCC | 4 cut(s) | 274, 281, 299, 640 |
| BshNI | GGYRCC | 1 cut(s) | 623 |
| BshVI | ATCGAT | 1 cut(s) | 579 |
| BsiHKAI | GWGCWC | 1 cut(s) | 216 |
| BsiHKCI | CYCGRG | 3 cut(s) | 254, 780, 802 |
| BsiSI | CCGG | 1 cut(s) | 647 |
| BslFI | GGGAC | 1 cut(s) | 740 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 200, 306, 329, 364 |
| BsmAI | GTCTC | 5 cut(s) | 474, 713, 783, 854, 1175 |
| BsmFI | GGGAC | 1 cut(s) | 740 |
| BsnI | GGCC | 4 cut(s) | 274, 281, 299, 640 |
| BsoBI | CYCGRG | 3 cut(s) | 254, 780, 802 |
| Bsp1286I | GDGCHC | 4 cut(s) | 171, 216, 669, 825 |
| Bsp143I | GATC | 3 cut(s) | 83, 790, 927 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 240 |
| Bsp19I | CCATGG | 1 cut(s) | 1107 |
| BspACI | CCGC | 2 cut(s) | 420, 876 |
| BspANI | GGCC | 4 cut(s) | 274, 281, 299, 640 |
| BspCNI | CTCAG | 5 cut(s) | 136, 183, 232, 892, 1129 |
| BspDI | ATCGAT | 1 cut(s) | 579 |
| BspFNI | CGCG | 1 cut(s) | 876 |
| BspHI | TCATGA | 1 cut(s) | 184 |
| BspLI | GGNNCC | 3 cut(s) | 625, 728, 729 |
| BspMAI | CTGCAG | 1 cut(s) | 1270 |
| BspPI | GGATC | 3 cut(s) | 91, 785, 935 |
| BspQI | GCTCTTC | 3 cut(s) | 204, 674, 1245 |
| BspT107I | GGYRCC | 1 cut(s) | 623 |
| BsrDI | GCAATG | 4 cut(s) | 447, 756, 1023, 1300 |
| BsrI | ACTGG | 4 cut(s) | 292, 323, 625, 667 |
| BssECI | CCNNGG | 1 cut(s) | 1107 |
| BssMI | GATC | 3 cut(s) | 83, 790, 927 |
| BssNAI | GTATAC | 1 cut(s) | 596 |
| BssT1I | CCWWGG | 1 cut(s) | 1107 |
| Bst1107I | GTATAC | 1 cut(s) | 596 |
| Bst2UI | CCWGG | 3 cut(s) | 365, 519, 642 |
| Bst4CI | ACNGT | 3 cut(s) | 499, 817, 865 |
| Bst6I | CTCTTC | 4 cut(s) | 204, 519, 674, 1245 |
| BstAPI | GCANNNNNTGC | 2 cut(s) | 1096, 1262 |
| BstBAI | YACGTR | 1 cut(s) | 1048 |
| BstC8I | GCNNGC | 3 cut(s) | 414, 418, 951 |
| BstDEI | CTNAG | 6 cut(s) | 144, 170, 240, 335, 900, 1137 |
| BstDSI | CCRYGG | 1 cut(s) | 1107 |
| BstF5I | GGATG | 3 cut(s) | 165, 355, 1294 |
| BstFNI | CGCG | 1 cut(s) | 876 |
| BstHHI | GCGC | 1 cut(s) | 961 |
| BstKTI | GATC | 3 cut(s) | 86, 793, 930 |
| BstMAI | GTCTC | 5 cut(s) | 474, 713, 783, 854, 1175 |
| BstMBI | GATC | 3 cut(s) | 83, 790, 927 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 1096, 1262 |
| BstNI | CCWGG | 3 cut(s) | 365, 519, 642 |
| BstNSI | RCATGY | 3 cut(s) | 416, 487, 602 |
| BstSCI | CCNGG | 3 cut(s) | 363, 517, 640 |
| BstSFI | CTRYAG | 1 cut(s) | 1266 |
| BstSLI | GKGCMC | 1 cut(s) | 825 |
| BstUI | CGCG | 1 cut(s) | 876 |
| BstV2I | GAAGAC | 1 cut(s) | 1310 |
| BstX2I | RGATCY | 1 cut(s) | 790 |
| BstYI | RGATCY | 1 cut(s) | 790 |
| BstZ17I | GTATAC | 1 cut(s) | 596 |
| Bsu15I | ATCGAT | 1 cut(s) | 579 |
| BsuI | GTATCC | 2 cut(s) | 201, 778 |
| BsuRI | GGCC | 4 cut(s) | 274, 281, 299, 640 |
| BsuTUI | ATCGAT | 1 cut(s) | 579 |
| BtgI | CCRYGG | 1 cut(s) | 1107 |
| BtsCI | GGATG | 3 cut(s) | 165, 355, 1294 |
| Cac8I | GCNNGC | 3 cut(s) | 414, 418, 951 |
| CaiI | CAGNNNCTG | 1 cut(s) | 863 |
| CciI | TCATGA | 1 cut(s) | 184 |
| CfoI | GCGC | 1 cut(s) | 961 |
| Cfr13I | GGNCC | 3 cut(s) | 644, 727, 1066 |
| ClaI | ATCGAT | 1 cut(s) | 579 |
| Csp6I | GTAC | 5 cut(s) | 265, 614, 650, 660, 969 |
| CviQI | GTAC | 5 cut(s) | 265, 614, 650, 660, 969 |
| DdeI | CTNAG | 6 cut(s) | 144, 170, 240, 335, 900, 1137 |
| DpnI | GATC | 3 cut(s) | 85, 792, 929 |
| DpnII | GATC | 3 cut(s) | 83, 790, 927 |
| EaeI | YGGCCR | 1 cut(s) | 279 |
| Eam1104I | CTCTTC | 4 cut(s) | 204, 519, 674, 1245 |
| EarI | CTCTTC | 4 cut(s) | 204, 519, 674, 1245 |
| Eco130I | CCWWGG | 1 cut(s) | 1107 |
| Eco147I | AGGCCT | 1 cut(s) | 640 |
| Eco24I | GRGCYC | 2 cut(s) | 171, 669 |
| Eco32I | GATATC | 1 cut(s) | 577 |
| Eco47I | GGWCC | 3 cut(s) | 644, 727, 1066 |
| Eco57I | CTGAAG | 2 cut(s) | 78, 877 |
| Eco72I | CACGTG | 1 cut(s) | 1048 |
| Eco88I | CYCGRG | 3 cut(s) | 254, 780, 802 |
| EcoO109I | RGGNCCY | 2 cut(s) | 727, 1066 |
| EcoRII | CCWGG | 3 cut(s) | 363, 517, 640 |
| EcoRV | GATATC | 1 cut(s) | 577 |
| EcoT14I | CCWWGG | 1 cut(s) | 1107 |
| EcoT22I | ATGCAT | 1 cut(s) | 414 |
| EcoT38I | GRGCYC | 2 cut(s) | 171, 669 |
| ErhI | CCWWGG | 1 cut(s) | 1107 |
| FaqI | GGGAC | 1 cut(s) | 740 |
| FauNDI | CATATG | 1 cut(s) | 549 |
| FblI | GTMKAC | 1 cut(s) | 595 |
| FokI | GGATG | 3 cut(s) | 172, 362, 1301 |
| FriOI | GRGCYC | 2 cut(s) | 171, 669 |
| FspBI | CTAG | 5 cut(s) | 114, 222, 371, 506, 723 |
| FspI | TGCGCA | 1 cut(s) | 960 |
| GlaI | GCGC | 1 cut(s) | 960 |
| GsaI | CCCAGC | 1 cut(s) | 203 |
| GsuI | CTGGAG | 2 cut(s) | 347, 540 |
| HaeIII | GGCC | 4 cut(s) | 274, 281, 299, 640 |
| HapII | CCGG | 1 cut(s) | 647 |
| HhaI | GCGC | 1 cut(s) | 961 |
| Hin6I | GCGC | 1 cut(s) | 959 |
| HinP1I | GCGC | 1 cut(s) | 959 |
| HinfI | GANTC | 6 cut(s) | 128, 181, 428, 800, 1144, 1183 |
| HpaII | CCGG | 1 cut(s) | 647 |
| HphI | GGTGA | 2 cut(s) | 7, 1073 |
| Hpy166II | GTNNAC | 3 cut(s) | 227, 596, 982 |
| Hpy188I | TCNGA | 4 cut(s) | 88, 97, 901, 1188 |
| Hpy188III | TCNNGA | 9 cut(s) | 172, 185, 194, 256, 425, 589, 782, 797, 804 |
| Hpy8I | GTNNAC | 3 cut(s) | 227, 596, 982 |
| Hpy99I | CGWCG | 1 cut(s) | 1232 |
| HpyAV | CCTTC | 2 cut(s) | 28, 484 |
| HpyCH4III | ACNGT | 3 cut(s) | 499, 817, 865 |
| HpyCH4IV | ACGT | 1 cut(s) | 1047 |
| HpyCH4V | TGCA | 6 cut(s) | 412, 571, 740, 1090, 1207, 1268 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 1096, 1262 |
| HpyF3I | CTNAG | 6 cut(s) | 144, 170, 240, 335, 900, 1137 |
| HpySE526I | ACGT | 1 cut(s) | 1047 |
| HspAI | GCGC | 1 cut(s) | 959 |
| KflI | GGGWCCC | 1 cut(s) | 727 |
| Kzo9I | GATC | 3 cut(s) | 83, 790, 927 |
| LguI | GCTCTTC | 3 cut(s) | 204, 674, 1245 |
| LmnI | GCTCC | 6 cut(s) | 118, 452, 704, 715, 888, 1250 |
| LweI | GCATC | 4 cut(s) | 340, 399, 571, 970 |
| MaeI | CTAG | 5 cut(s) | 114, 222, 371, 506, 723 |
| MaeII | ACGT | 1 cut(s) | 1047 |
| MaeIII | GTNAC | 4 cut(s) | 422, 530, 811, 1270 |
| MalI | GATC | 3 cut(s) | 85, 792, 929 |
| MboI | GATC | 3 cut(s) | 83, 790, 927 |
| MboII | GAAGA | 6 cut(s) | 221, 536, 661, 1063, 1153, 1232 |
| MflI | RGATCY | 1 cut(s) | 790 |
| MhlI | GDGCHC | 4 cut(s) | 171, 216, 669, 825 |
| MlsI | TGGCCA | 1 cut(s) | 281 |
| MluCI | AATT | 1 cut(s) | 1275 |
| MluNI | TGGCCA | 1 cut(s) | 281 |
| MlyI | GAGTC | 2 cut(s) | 794, 1177 |
| MmeI | TCCRAC | 2 cut(s) | 790, 943 |
| Mox20I | TGGCCA | 1 cut(s) | 281 |
| Mph1103I | ATGCAT | 1 cut(s) | 414 |
| MscI | TGGCCA | 1 cut(s) | 281 |
| MseI | TTAA | 3 cut(s) | 554, 1023, 1260 |
| MslI | CAYNNNNRTG | 1 cut(s) | 828 |
| Msp20I | TGGCCA | 1 cut(s) | 281 |
| MspA1I | CMGCKG | 1 cut(s) | 956 |
| MspI | CCGG | 1 cut(s) | 647 |
| MspR9I | CCNGG | 3 cut(s) | 365, 519, 642 |
| MvaI | CCWGG | 3 cut(s) | 365, 519, 642 |
| MvnI | CGCG | 1 cut(s) | 876 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 1096, 1262 |
| NcoI | CCATGG | 1 cut(s) | 1107 |
| NdeI | CATATG | 1 cut(s) | 549 |
| NdeII | GATC | 3 cut(s) | 83, 790, 927 |
| NlaIV | GGNNCC | 3 cut(s) | 625, 728, 729 |
| NmeAIII | GCCGAG | 1 cut(s) | 653 |
| NmuCI | GTSAC | 1 cut(s) | 422 |
| NsbI | TGCGCA | 1 cut(s) | 960 |
| NsiI | ATGCAT | 1 cut(s) | 414 |
| NspI | RCATGY | 3 cut(s) | 416, 487, 602 |
| PaeI | GCATGC | 1 cut(s) | 416 |
| PaeR7I | CTCGAG | 1 cut(s) | 802 |
| PagI | TCATGA | 1 cut(s) | 184 |
| PceI | AGGCCT | 1 cut(s) | 640 |
| PciI | ACATGT | 2 cut(s) | 483, 598 |
| PciSI | GCTCTTC | 3 cut(s) | 204, 674, 1245 |
| PfeI | GAWTC | 4 cut(s) | 128, 181, 428, 1144 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 329 |
| PfoI | TCCNGGA | 1 cut(s) | 363 |
| PleI | GAGTC | 2 cut(s) | 794, 1177 |
| PmaCI | CACGTG | 1 cut(s) | 1048 |
| PmlI | CACGTG | 1 cut(s) | 1048 |
| PpsI | GAGTC | 2 cut(s) | 794, 1177 |
| Ppu21I | YACGTR | 1 cut(s) | 1048 |
| PpuMI | RGGWCCY | 2 cut(s) | 727, 1066 |
| PscI | ACATGT | 2 cut(s) | 483, 598 |
| Psp5II | RGGWCCY | 2 cut(s) | 727, 1066 |
| Psp6I | CCWGG | 3 cut(s) | 363, 517, 640 |
| PspCI | CACGTG | 1 cut(s) | 1048 |
| PspFI | CCCAGC | 1 cut(s) | 199 |
| PspGI | CCWGG | 3 cut(s) | 363, 517, 640 |
| PspN4I | GGNNCC | 3 cut(s) | 625, 728, 729 |
| PspPI | GGNCC | 3 cut(s) | 644, 727, 1066 |
| PspPPI | RGGWCCY | 2 cut(s) | 727, 1066 |
| PstI | CTGCAG | 1 cut(s) | 1270 |
| PstNI | CAGNNNCTG | 1 cut(s) | 863 |
| PsuI | RGATCY | 1 cut(s) | 790 |
| PvuII | CAGCTG | 1 cut(s) | 956 |
| RsaI | GTAC | 5 cut(s) | 266, 615, 651, 661, 970 |
| RsaNI | GTAC | 5 cut(s) | 265, 614, 650, 660, 969 |
| RseI | CAYNNNNRTG | 1 cut(s) | 828 |
| SapI | GCTCTTC | 3 cut(s) | 204, 674, 1245 |
| SaqAI | TTAA | 3 cut(s) | 554, 1023, 1260 |
| Sau3AI | GATC | 3 cut(s) | 83, 790, 927 |
| Sau96I | GGNCC | 3 cut(s) | 644, 727, 1066 |
| SchI | GAGTC | 2 cut(s) | 794, 1177 |
| ScrFI | CCNGG | 3 cut(s) | 365, 519, 642 |
| SduI | GDGCHC | 4 cut(s) | 171, 216, 669, 825 |
| SfaNI | GCATC | 4 cut(s) | 340, 399, 571, 970 |
| SfcI | CTRYAG | 1 cut(s) | 1266 |
| Sfr274I | CTCGAG | 1 cut(s) | 802 |
| SinI | GGWCC | 3 cut(s) | 644, 727, 1066 |
| SlaI | CTCGAG | 1 cut(s) | 802 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 828 |
| SmlI | CTYRAG | 3 cut(s) | 101, 589, 802 |
| SmoI | CTYRAG | 3 cut(s) | 101, 589, 802 |
| SpeI | ACTAGT | 3 cut(s) | 221, 370, 505 |
| SphI | GCATGC | 1 cut(s) | 416 |
| Sse9I | AATT | 1 cut(s) | 1275 |
| SseBI | AGGCCT | 1 cut(s) | 640 |
| SsiI | CCGC | 2 cut(s) | 420, 876 |
| SspMI | CTAG | 5 cut(s) | 114, 222, 371, 506, 723 |
| StuI | AGGCCT | 1 cut(s) | 640 |
| StyD4I | CCNGG | 3 cut(s) | 363, 517, 640 |
| StyI | CCWWGG | 1 cut(s) | 1107 |
| TaaI | ACNGT | 3 cut(s) | 499, 817, 865 |
| TaiI | ACGT | 1 cut(s) | 1050 |
| TaqI | TCGA | 4 cut(s) | 579, 635, 803, 1227 |
| TasI | AATT | 1 cut(s) | 1275 |
| TatI | WGTACW | 2 cut(s) | 264, 968 |
| TfiI | GAWTC | 4 cut(s) | 128, 181, 428, 1144 |
| Tru1I | TTAA | 3 cut(s) | 554, 1023, 1260 |
| Tru9I | TTAA | 3 cut(s) | 554, 1023, 1260 |
| TseFI | GTSAC | 1 cut(s) | 422 |
| Tsp45I | GTSAC | 1 cut(s) | 422 |
| TspDTI | ATGAA | 5 cut(s) | 38, 173, 976, 1044, 1269 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 329 |
| VpaK11BI | GGWCC | 3 cut(s) | 644, 727, 1066 |
| XceI | RCATGY | 3 cut(s) | 416, 487, 602 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 289 |
| XhoI | CTCGAG | 1 cut(s) | 802 |
| XmiI | GTMKAC | 1 cut(s) | 595 |
| XspI | CTAG | 5 cut(s) | 114, 222, 371, 506, 723 |
| Zsp2I | ATGCAT | 1 cut(s) | 414 |