Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000007

Orthogroup ID

OG0000227

Physical Location & Seq

Genomic Coordinates Reverse (-)

58257342 .. 58260499

Gene Structure 3158 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_7G00208260.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 900 bp

ATGTTCGAGGTCAACTCCGACCGGACTTGGGTGCTATTTTCATCAACCACAATCGGTTCGAGTTCGATCCGGACTCGTCCCAATGATGTTCTTGTTCAACCTTCGAACCCATCTCCACTTCTCTTCGAGCTTATCGATCTTGTTGTGGCACCGCATGGCGGTCAATGGGAATTGGGCGGCGAGGGTGGTGGTGACGTCATCCCGATTGGTGGCTTTGAGATTGGTGTGGTCGAGAGAGGACCACTTGTCCTTGAAGATGGAGATGAGGGCTTCGGCCTCGTTGTCCTGTTGGTGGAGGAAATACTAAGAGTTCAACCAAACGTGAAAAAGCTGTATCTTCTTTTAAGAGCTTCCGATGCCAAATGTGCCGCACATCGCATGCATAATGAGATCACAGGAAAAGAGTTGTTCAGGATTTTAAGGGAGAAATGGGGCACAAGTTTTGATTCCTTCATGTCAGAAAAGATTGTTGCTCTCTCAGGAGACGCAACTTTTAAAGACCTGGGAGTGAAAGAATTCAAAATAAGAGAAGAAATGTGCAACGCAGTACAAATTATAATTAATTCTGCAGCTAGCACCAAGTGGAATGAAAGGTATGACGTTGCACTGGAAGTGAATACATTTGGAGTTTTGAATGTGTTAGACTTTGCAAAGAAATGCTTAAATCCGGAGATGGTTGTTCACGTGTCAACAACTTATGTGTCTGCTGATAGGGCAGGGCTGATACTAGAGGACTCATCTTCAATGGATGAGATGGCAAGACAAACTACTCATGATGTTGACTTGAAAGAACATGAAAAGAAGATGGTGGAGCGCAAATTGAAAGAACGACAAGCACAGCACGCTTCTGAAGAAGTTATTACAAATACAATGAAGGACTTTGGCACTGCAAGGTTTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005886 GO:0006139 GO:0006163 GO:0006637 GO:0006725 GO:0006732 GO:0006753 GO:0006790 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006970 GO:0008150 GO:0008152 GO:0009058 GO:0009117 GO:0009150 GO:0009259 GO:0009507 GO:0009536 GO:0009611 GO:0009628 GO:0009651 GO:0009698 GO:0009699 GO:0009987 GO:0010345 GO:0016020 GO:0016491 GO:0016620 GO:0016903 GO:0019438 GO:0019637 GO:0019693 GO:0019748 GO:0033865 GO:0033875 GO:0034032 GO:0034641 GO:0035336 GO:0035337 GO:0035383 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043603 GO:0044237 GO:0044238 GO:0044249 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0044550 GO:0046483 GO:0050062 GO:0050896 GO:0051186 GO:0055086 GO:0055114 GO:0071704 GO:0071944 GO:0072521 GO:0080019 GO:1901135 GO:1901360 GO:1901362 GO:1901564 GO:1901568 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00073 ko04146 ko04212 map00073 map04146 map04212

Pfam Domains

Protein Families

NAD_binding_4 Sterile

Protein Analysis

299

Amino Acids

33.34

Weight (kDa)

5.16

Isoelectric Point (pI)

33.87

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
NAD_binding_4	PF07993	95 - 267	3.7e-35	Male sterility protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000227)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G44550 AT3G44550 AT3G44560 AT3G44560 AT3G44560
fragaria_vesca	FvH4_3g09600 FvH4_3g24560 FvH4_3g24560 FvH4_3g24560 FvH4_3g24570 FvH4_3g24570 FvH4_3g38410 FvH4_3g38410 FvH4_3g38410 FvH4_5g01800 FvH4_7g07480 FvH4_7g07480
malus_domestica	MD02G1239300.v1.1 MD03G1141400.v1.1 MD03G1141600.v1.1 MD03G1141700.v1.1 MD10G1066600.v1.1 MD11G1171600.v1.1
prunus_persica	Prupe.6G126600_v2.0.a1 Prupe.6G126700_v2.0.a1 Prupe.6G126700_v2.0.a1 Prupe.6G126800_v2.0.a1
pyrus_communis	pycom03g09610 pycom03g09620 pycom03g09650 pycom10g05210 pycom11g13330
rosa_chinensis	RchiOBHm_Chr3g0483921 RchiOBHm_Chr5g0043321 RchiOBHm_Chr5g0044031 RchiOBHm_Chr5g0044071 RchiOBHm_Chr5g0069061 RchiOBHm_Chr5g0069111 RchiOBHm_Chr5g0069131 RchiOBHm_Chr6g0256891 RchiOBHm_Chr6g0256901 RchiOBHm_Chr7g0198821 RchiOBHm_Chr7g0198851 RchiOBHm_Chr7g0198861 RchiOBHm_Chr7g0219381 RchiOBHm_Chr7g0230491
rosa_laevigata	RLG00000001491 RLG00000003837 RLG00000014654 RLG00000014655 RLG00000020958 RLG00000023252 RLG00000028966 RLG00000034179 RLG00000034211 RLG00000034236 RLG00000034238 RLG00000036034 RLG00000036038
rosa_multiflora	Rmu_co8020118.1_g000001 Rmu_sc0000060.1_g000002 Rmu_sc0000060.1_g000026 Rmu_sc0001157.1_g000010 Rmu_sc0001157.1_g000016 Rmu_sc0002252.1_g000009 Rmu_sc0003076.1_g000025 Rmu_sc0008623.1_g000018 Rmu_sc0008623.1_g000021 Rmu_sc0022203.1_g000001
rosa_roxburghii	Rroxscaffold_1G00036940 Rroxscaffold_1G00036990 Rroxscaffold_3G00230110 Rroxscaffold_3G00257340 Rroxscaffold_3G00257380 Rroxscaffold_6G00398020 Rroxscaffold_6G00398040 Rroxscaffold_7G00208240 Rroxscaffold_7G00208260
rosa_rugosa	Rorug01G0059500 Rorug03G0208700 Rorug03G0208700 Rorug03G0208800 Rorug03G0208900 Rorug03G0208900 Rorug04G0119300 Rorug05G0210300 Rorug05G0210300 Rorug05G0395800 Rorug05G0396000 Rorug05G0396100 Rorug05G0396200 Rorug05G0577200 Rorug07G0050100 Rorug07G0050200 Rorug07G0215200
rosa_samantha	Rh1BG061200 Rh2BG536700 Rh2DG545600 Rh3AG257500 Rh3BG294300 Rh3BG322600 Rh3CG292100 Rh3DG287300 Rh4BG178300 Rh5AG290800 Rh5AG291000 Rh5AG294200 Rh5AG294300 Rh5BG297400 Rh5BG302600 Rh5BG303000 Rh5CG329900 Rh5CG330100 Rh5DG307000 Rh5DG311500 Rh5DG311700 Rh6AG095800 Rh6BG086800 Rh6BG086900 Rh6CG082900 Rh6DG078600 Rh6DG078700 Rh7AG173700 Rh7AG174000 Rh7AG174100 Rh7AG327500 Rh7AG412100 Rh7BG177100 Rh7CG182900 Rh7CG183100 Rh7CG344800 Rh7CG429900 Rh7DG176600
rosa_wichuraiana	Rw0G003880 Rw0G006090 Rw2G043200 Rw3G023270 Rw3G023310 Rw5G026990 Rw5G027280 Rw5G027300 Rw5G042270 Rw6G008160 Rw7G015210 Rw7G015230

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	557
AatII	GACGTC	1 cut(s)	198
AccB1I	GGYRCC	1 cut(s)	148
AccIII	TCCGGA	2 cut(s)	69, 667
AciI	CCGC	4 cut(s)	152, 159, 177, 369
AclWI	GGATC	1 cut(s)	61
AcsI	RAATTY	1 cut(s)	515
AcuI	CTGAAG	1 cut(s)	870
AcvI	CACGTG	1 cut(s)	685
AcyI	GRCGYC	1 cut(s)	195
AdeI	CACNNNGTG	1 cut(s)	582
AfaI	GTAC	1 cut(s)	549
AfiI	CCNNNNNNNGG	4 cut(s)	28, 158, 209, 292
AflIII	ACRYGT	1 cut(s)	684
AgsI	TTSAA	8 cut(s)	98, 254, 314, 520, 634, 744, 787, 823
AhdI	GACNNNNNGTC	1 cut(s)	245
AjnI	CCWGG	1 cut(s)	501
AluBI	AGCT	4 cut(s)	130, 331, 350, 572
AluI	AGCT	4 cut(s)	130, 331, 350, 572
Alw26I	GTCTC	1 cut(s)	477
AlwI	GGATC	1 cut(s)	61
Aor13HI	TCCGGA	2 cut(s)	69, 667
AoxI	GGCC	1 cut(s)	274
ApeKI	GCWGC	1 cut(s)	569
ApoI	RAATTY	1 cut(s)	515
AseI	ATTAAT	1 cut(s)	561
Asp700I	GAANNNNTTC	1 cut(s)	515
AspLEI	GCGC	1 cut(s)	816
AspS9I	GGNCC	1 cut(s)	239
AsuHPI	GGTGA	1 cut(s)	203
AsuII	TTCGAA	1 cut(s)	104
AsuNHI	GCTAGC	1 cut(s)	572
AvaII	GGWCC	1 cut(s)	239
BaeGI	GKGCMC	1 cut(s)	437
BanI	GGYRCC	1 cut(s)	148
BbrPI	CACGTG	1 cut(s)	685
BbvI	GCAGC	1 cut(s)	581
BccI	CCATC	5 cut(s)	118, 251, 667, 748, 799
BciT130I	CCWGG	1 cut(s)	503
BcoDI	GTCTC	1 cut(s)	477
BfaI	CTAG	2 cut(s)	573, 728
BfmI	CTRYAG	1 cut(s)	567
BisI	GCNGC	3 cut(s)	178, 369, 570
BlsI	GCNGC	3 cut(s)	179, 370, 571
Bme1390I	CCNGG	1 cut(s)	503
Bme18I	GGWCC	1 cut(s)	239
BmeRI	GACNNNNNGTC	1 cut(s)	245
BmgT120I	GGNCC	1 cut(s)	239
BmiI	GGNNCC	1 cut(s)	150
BmrFI	CCNGG	1 cut(s)	503
BmsI	GCATC	1 cut(s)	346
BmtI	GCTAGC	1 cut(s)	576
BplI	GAGNNNNNCTC	1 cut(s)	31
Bpu14I	TTCGAA	1 cut(s)	104
Bsa29I	ATCGAT	1 cut(s)	135
BsaAI	YACGTR	1 cut(s)	685
BsaHI	GRCGYC	1 cut(s)	195
BsaJI	CCNNGG	1 cut(s)	502
BsaWI	WCCGGW	3 cut(s)	21, 69, 667
Bsc4I	CCNNNNNNNGG	4 cut(s)	28, 158, 209, 292
Bse1I	ACTGG	1 cut(s)	612
BseAI	TCCGGA	2 cut(s)	69, 667
BseBI	CCWGG	1 cut(s)	503
BseCI	ATCGAT	1 cut(s)	135
BseDI	CCNNGG	1 cut(s)	502
BseGI	GGATG	2 cut(s)	198, 754
BseLI	CCNNNNNNNGG	4 cut(s)	28, 158, 209, 292
BseMII	CTCAG	1 cut(s)	492
BseNI	ACTGG	1 cut(s)	612
BseSI	GKGCMC	1 cut(s)	437
BseXI	GCAGC	1 cut(s)	581
Bsh1285I	CGRYCG	1 cut(s)	22
BshFI	GGCC	1 cut(s)	276
BshNI	GGYRCC	1 cut(s)	148
BshVI	ATCGAT	1 cut(s)	135
BsiEI	CGRYCG	1 cut(s)	22
BsiSI	CCGG	3 cut(s)	22, 70, 668
BslFI	GGGAC	1 cut(s)	63
BslI	CCNNNNNNNGG	4 cut(s)	28, 158, 209, 292
BsmAI	GTCTC	1 cut(s)	477
BsmBI	CGTCTC	1 cut(s)	477
BsmFI	GGGAC	1 cut(s)	63
BsnI	GGCC	1 cut(s)	276
Bsp119I	TTCGAA	1 cut(s)	104
Bsp1286I	GDGCHC	1 cut(s)	437
Bsp13I	TCCGGA	2 cut(s)	69, 667
Bsp143I	GATC	3 cut(s)	66, 136, 390
BspACI	CCGC	4 cut(s)	152, 159, 177, 369
BspANI	GGCC	1 cut(s)	276
BspCNI	CTCAG	1 cut(s)	491
BspDI	ATCGAT	1 cut(s)	135
BspEI	TCCGGA	2 cut(s)	69, 667
BspHI	TCATGA	1 cut(s)	772
BspLI	GGNNCC	1 cut(s)	150
BspMAI	CTGCAG	1 cut(s)	571
BspOI	GCTAGC	1 cut(s)	576
BspPI	GGATC	1 cut(s)	61
BspT104I	TTCGAA	1 cut(s)	104
BspT107I	GGYRCC	1 cut(s)	148
BsrI	ACTGG	1 cut(s)	612
BssECI	CCNNGG	1 cut(s)	502
BssMI	GATC	3 cut(s)	66, 136, 390
BssNI	GRCGYC	1 cut(s)	195
Bst2UI	CCWGG	1 cut(s)	503
Bst6I	CTCTTC	1 cut(s)	128
BstACI	GRCGYC	1 cut(s)	195
BstBAI	YACGTR	1 cut(s)	685
BstBI	TTCGAA	1 cut(s)	104
BstC8I	GCNNGC	3 cut(s)	380, 574, 843
BstDEI	CTNAG	2 cut(s)	305, 478
BstF5I	GGATG	2 cut(s)	198, 754
BstHHI	GCGC	1 cut(s)	816
BstKTI	GATC	3 cut(s)	69, 139, 393
BstMAI	GTCTC	1 cut(s)	477
BstMBI	GATC	3 cut(s)	66, 136, 390
BstMCI	CGRYCG	1 cut(s)	22
BstMWI	GCNNNNNNNGC	4 cut(s)	356, 365, 713, 842
BstNI	CCWGG	1 cut(s)	503
BstNSI	RCATGY	1 cut(s)	382
BstSCI	CCNGG	1 cut(s)	501
BstSFI	CTRYAG	1 cut(s)	567
BstSLI	GKGCMC	1 cut(s)	437
BstV1I	GCAGC	1 cut(s)	581
Bsu15I	ATCGAT	1 cut(s)	135
BsuRI	GGCC	1 cut(s)	276
BsuTUI	ATCGAT	1 cut(s)	135
BtgZI	GCGATG	1 cut(s)	359
BtsCI	GGATG	2 cut(s)	198, 754
BtsI	GCAGTG	1 cut(s)	885
BtsIMutI	CAGTG	2 cut(s)	605, 885
Cac8I	GCNNGC	3 cut(s)	380, 574, 843
CciI	TCATGA	1 cut(s)	772
CfoI	GCGC	1 cut(s)	816
Cfr13I	GGNCC	1 cut(s)	239
ClaI	ATCGAT	1 cut(s)	135
CseI	GACGC	1 cut(s)	494
Csp6I	GTAC	1 cut(s)	548
CviAII	CATG	5 cut(s)	155, 379, 454, 773, 794
CviJI	RGCY	8 cut(s)	130, 213, 270, 276, 331, 350, 572, 721
CviKI_1	RGCY	8 cut(s)	130, 213, 270, 276, 331, 350, 572, 721
CviQI	GTAC	1 cut(s)	548
DdeI	CTNAG	2 cut(s)	305, 478
DpnI	GATC	3 cut(s)	68, 138, 392
DpnII	GATC	3 cut(s)	66, 136, 390
DraI	TTTAAA	1 cut(s)	496
DraIII	CACNNNGTG	1 cut(s)	582
DriI	GACNNNNNGTC	1 cut(s)	245
Eam1104I	CTCTTC	1 cut(s)	128
Eam1105I	GACNNNNNGTC	1 cut(s)	245
EarI	CTCTTC	1 cut(s)	128
Eco47I	GGWCC	1 cut(s)	239
Eco57I	CTGAAG	1 cut(s)	870
Eco72I	CACGTG	1 cut(s)	685
EcoRI	GAATTC	1 cut(s)	515
EcoRII	CCWGG	1 cut(s)	501
EcoT22I	ATGCAT	1 cut(s)	384
Esp3I	CGTCTC	1 cut(s)	477
FaeI	CATG	5 cut(s)	158, 382, 457, 776, 797
FaiI	YATR	9 cut(s)	156, 380, 384, 455, 557, 597, 699, 774, 795
FalI	AAGNNNNNCTT	2 cut(s)	644, 676
FaqI	GGGAC	1 cut(s)	63
FatI	CATG	5 cut(s)	154, 378, 453, 772, 793
Fnu4HI	GCNGC	3 cut(s)	178, 369, 570
FokI	GGATG	2 cut(s)	185, 761
Fsp4HI	GCNGC	3 cut(s)	178, 369, 570
FspBI	CTAG	2 cut(s)	573, 728
GlaI	GCGC	1 cut(s)	815
GluI	GCNGC	3 cut(s)	178, 369, 570
HaeIII	GGCC	1 cut(s)	276
HapII	CCGG	3 cut(s)	22, 70, 668
HgaI	GACGC	1 cut(s)	494
HhaI	GCGC	1 cut(s)	816
Hin1I	GRCGYC	1 cut(s)	195
Hin1II	CATG	5 cut(s)	158, 382, 457, 776, 797
Hin6I	GCGC	1 cut(s)	814
HinP1I	GCGC	1 cut(s)	814
HincII	GTYRAC	3 cut(s)	13, 690, 781
HindII	GTYRAC	3 cut(s)	13, 690, 781
HinfI	GANTC	3 cut(s)	73, 446, 734
HpaII	CCGG	3 cut(s)	22, 70, 668
HphI	GGTGA	1 cut(s)	203
Hpy166II	GTNNAC	4 cut(s)	13, 682, 690, 781
Hpy188I	TCNGA	4 cut(s)	19, 355, 460, 850
Hpy188III	TCNNGA	7 cut(s)	70, 202, 232, 412, 480, 668, 773
Hpy8I	GTNNAC	4 cut(s)	13, 682, 690, 781
HpyAV	CCTTC	3 cut(s)	111, 460, 868
HpyCH4IV	ACGT	4 cut(s)	195, 321, 600, 684
HpyCH4V	TGCA	6 cut(s)	382, 540, 569, 605, 650, 890
HpyF10VI	GCNNNNNNNGC	4 cut(s)	356, 365, 713, 842
HpyF3I	CTNAG	2 cut(s)	305, 478
HpySE526I	ACGT	4 cut(s)	195, 321, 600, 684
Hsp92I	GRCGYC	1 cut(s)	195
Hsp92II	CATG	5 cut(s)	158, 382, 457, 776, 797
HspAI	GCGC	1 cut(s)	814
Kpn2I	TCCGGA	2 cut(s)	69, 667
Kzo9I	GATC	3 cut(s)	66, 136, 390
LmnI	GCTCC	1 cut(s)	811
Lsp1109I	GCAGC	1 cut(s)	581
LweI	GCATC	1 cut(s)	346
MaeI	CTAG	2 cut(s)	573, 728
MaeII	ACGT	4 cut(s)	195, 321, 600, 684
MaeIII	GTNAC	1 cut(s)	191
MalI	GATC	3 cut(s)	68, 138, 392
MboI	GATC	3 cut(s)	66, 136, 390
MboII	GAAGA	7 cut(s)	115, 266, 329, 542, 732, 814, 863
MhlI	GDGCHC	1 cut(s)	437
MluCI	AATT	6 cut(s)	170, 515, 552, 558, 562, 818
MlyI	GAGTC	2 cut(s)	67, 728
MmeI	TCCRAC	1 cut(s)	42
MnlI	CCTC	6 cut(s)	175, 230, 259, 287, 289, 724
Mph1103I	ATGCAT	1 cut(s)	384
MroI	TCCGGA	2 cut(s)	69, 667
MroXI	GAANNNNTTC	1 cut(s)	515
MseI	TTAA	5 cut(s)	344, 419, 495, 561, 662
MspI	CCGG	3 cut(s)	22, 70, 668
MspR9I	CCNGG	1 cut(s)	503
MvaI	CCWGG	1 cut(s)	503
MwoI	GCNNNNNNNGC	4 cut(s)	356, 365, 713, 842
NdeII	GATC	3 cut(s)	66, 136, 390
NheI	GCTAGC	1 cut(s)	572
NlaIII	CATG	5 cut(s)	158, 382, 457, 776, 797
NlaIV	GGNNCC	1 cut(s)	150
NmuCI	GTSAC	1 cut(s)	191
NsiI	ATGCAT	1 cut(s)	384
NspI	RCATGY	1 cut(s)	382
NspV	TTCGAA	1 cut(s)	104
PaeI	GCATGC	1 cut(s)	382
PagI	TCATGA	1 cut(s)	772
PcsI	WCGNNNNNNNCGW	1 cut(s)	132
PdmI	GAANNNNTTC	1 cut(s)	515
PfeI	GAWTC	1 cut(s)	446
PkrI	GCNGC	3 cut(s)	179, 370, 571
PleI	GAGTC	2 cut(s)	67, 728
PmaCI	CACGTG	1 cut(s)	685
PmlI	CACGTG	1 cut(s)	685
PpsI	GAGTC	2 cut(s)	67, 728
Ppu21I	YACGTR	1 cut(s)	685
PshBI	ATTAAT	1 cut(s)	561
PsiI	TTATAA	1 cut(s)	557
Psp6I	CCWGG	1 cut(s)	501
PspCI	CACGTG	1 cut(s)	685
PspGI	CCWGG	1 cut(s)	501
PspN4I	GGNNCC	1 cut(s)	150
PspPI	GGNCC	1 cut(s)	239
PstI	CTGCAG	1 cut(s)	571
RsaI	GTAC	1 cut(s)	549
RsaNI	GTAC	1 cut(s)	548
SaqAI	TTAA	5 cut(s)	344, 419, 495, 561, 662
SatI	GCNGC	3 cut(s)	178, 369, 570
Sau3AI	GATC	3 cut(s)	66, 136, 390
Sau96I	GGNCC	1 cut(s)	239
SchI	GAGTC	2 cut(s)	67, 728
ScrFI	CCNGG	1 cut(s)	503
SduI	GDGCHC	1 cut(s)	437
SfaNI	GCATC	1 cut(s)	346
SfcI	CTRYAG	1 cut(s)	567
SfuI	TTCGAA	1 cut(s)	104
SinI	GGWCC	1 cut(s)	239
SphI	GCATGC	1 cut(s)	382
Sse9I	AATT	6 cut(s)	170, 515, 552, 558, 562, 818
SsiI	CCGC	4 cut(s)	152, 159, 177, 369
SspMI	CTAG	2 cut(s)	573, 728
StyD4I	CCNGG	1 cut(s)	501
TaiI	ACGT	4 cut(s)	198, 324, 603, 687
TaqI	TCGA	7 cut(s)	6, 59, 65, 104, 126, 135, 231
TasI	AATT	6 cut(s)	170, 515, 552, 558, 562, 818
TatI	WGTACW	1 cut(s)	547
TauI	GCSGC	2 cut(s)	180, 371
TfiI	GAWTC	1 cut(s)	446
Tru1I	TTAA	5 cut(s)	344, 419, 495, 561, 662
Tru9I	TTAA	5 cut(s)	344, 419, 495, 561, 662
TscAI	CASTG	2 cut(s)	612, 892
TseFI	GTSAC	1 cut(s)	191
TseI	GCWGC	1 cut(s)	569
Tsp45I	GTSAC	1 cut(s)	191
TspDTI	ATGAA	5 cut(s)	30, 442, 603, 810, 887
TspRI	CASTG	2 cut(s)	612, 892
VpaK11BI	GGWCC	1 cut(s)	239
VspI	ATTAAT	1 cut(s)	561
XapI	RAATTY	1 cut(s)	515
XceI	RCATGY	1 cut(s)	382
XmnI	GAANNNNTTC	1 cut(s)	515
XspI	CTAG	2 cut(s)	573, 728
ZraI	GACGTC	1 cut(s)	196
Zsp2I	ATGCAT	1 cut(s)	384

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.