Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000003

Orthogroup ID

OG0000227

Physical Location & Seq

Genomic Coordinates Forward (+)

14655610 .. 14708064

Gene Structure 52455 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_3G00230110.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1500 bp

ATGGAGTTGGAGAGCATTCGAGGATGTTTGGAGAACAAGACCGTCTTAGTTACTGGAGCCACTGGCTTGCTTGGAATGGTTTTTGTGGAGAAAATACTGAGAGTTCAGCCAAATGTAAAAAAGCTATACCTCCTCATTAGAGCTTCTGATGCCAGATCTGCCACACGTCGCATGCACAATGAGATCACAAGGAAAGAGCTGTTCAGAAATTTAAGGAAGAAATGGGGTGCAAATTTTGACTCCTTTGTATCAGAAAAAGTTGTTGCTCTTCCAGGAGACATAACATATACAAGTCTACTAGAAGCAAACGAAATCAAACTAAGGGACCAATTGTGCAATGAGATACAAATCATCCTTAACTCTGCAGCCACCCTCACTTTTGATGAGAGGTATGATGTTGCTCTGGACGTTAATACATTTGGAGTTTTGAATGTGCTGAGCTTTGCAAAAATGTGTTTAAGGCTAGAGATACTTGTTCACATATCTACTGCTTATGTATGTGGTGAAAAGGCTGGGCTCATAGTCGAGGATTTCTCTTTAATGGATGAGGTGGTAAAGAGAAGCACTAAATCCGACCTGGCAATAGTGGAAAAAACCATAGCAGACAAGATATTGAAAGAACTCAAAGCACAAGATACTTCTGAAAAATTCATTACAAATAGGATGAAGGACTTGGGCTCTACAAGGGCTAAGTTATATGGCTGGCCAAATACGTATGTGTTTACAAAAGCAATGGCAGAAGTATATCTAGAACACTCGAAAGGTAGTGTACCGATTACCATTATACGTCCAACTGTTGTTACTAGTACTTATAAGGAGCCATTTCCAGGATGGATTCAAGGTCTCAGAACCGTTGATAGTGTAATTAGTGGATACTGGAGAGGGAACTTGACATGCCTACCAATGGATCCTACCTCAGTGTTAGATATGATTCCAGTTGACATGCTAGTAAATTCAATAATTACAGCAATCGTAATGAATGGTAATAAATCTTCTGGAATCATCTACCATGTGGGGACCTCATTAAGAAACCCTATAAATATGTCCAAGATGATGAATTTTTTGTTCCAATACTGCACCACAAATCCACTGCTTCATAAGGATGGAAGCCCTATCAAGATTGACAGGCTGAAAATGTTCAAGACTATGGACACTTTTCGCTTCTACATGCAAATTCGCTTCATGCTACCATTAGAGGTAGTGAAGCTCCTAAATAAAGAATTTGACCAGTATTTCAGAGATGTGTACGTTAGCTATAATCGGAAGCTCACATCGGTGATGCGGTTAGCTGAGCTATATGAACCCTATCTTCTATTCAAGGCAATCTTTGACGACACCAATTCGGAGGAGTTGAGAAGGATGGCAAGTAAGAAATACACAGATGCCGAAATTTTCAATTTTGATCCAAGATGCATTAATTGGGAAGACTACGTTATGCGTACTCACATCCCTGGCCTCTGGAAAAACGTTATGACCAAGAAGAGTCACAGCCGGCTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005886 GO:0006139 GO:0006163 GO:0006637 GO:0006725 GO:0006732 GO:0006753 GO:0006790 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006970 GO:0008150 GO:0008152 GO:0009058 GO:0009117 GO:0009150 GO:0009259 GO:0009507 GO:0009536 GO:0009611 GO:0009628 GO:0009651 GO:0009698 GO:0009699 GO:0009987 GO:0010345 GO:0016020 GO:0016491 GO:0016620 GO:0016903 GO:0019438 GO:0019637 GO:0019693 GO:0019748 GO:0033865 GO:0033875 GO:0034032 GO:0034641 GO:0035336 GO:0035337 GO:0035383 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043603 GO:0044237 GO:0044238 GO:0044249 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0044550 GO:0046483 GO:0050062 GO:0050896 GO:0051186 GO:0055086 GO:0055114 GO:0071704 GO:0071944 GO:0072521 GO:0080019 GO:1901135 GO:1901360 GO:1901362 GO:1901564 GO:1901568 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00073 ko04146 ko04212 map00073 map04146 map04212

Pfam Domains

Protein Families

NAD_binding_4 Sterile

Protein Analysis

499

Amino Acids

57.41

Weight (kDa)

9.14

Isoelectric Point (pI)

32.76

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
NAD_binding_4	PF07993	17 - 320	2e-66	Male sterility protein
Sterile	PF03015	395 - 491	2e-14	Male sterility protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000227)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G44550 AT3G44550 AT3G44560 AT3G44560 AT3G44560
fragaria_vesca	FvH4_3g09600 FvH4_3g24560 FvH4_3g24560 FvH4_3g24560 FvH4_3g24570 FvH4_3g24570 FvH4_3g38410 FvH4_3g38410 FvH4_3g38410 FvH4_5g01800 FvH4_7g07480 FvH4_7g07480
malus_domestica	MD02G1239300.v1.1 MD03G1141400.v1.1 MD03G1141600.v1.1 MD03G1141700.v1.1 MD10G1066600.v1.1 MD11G1171600.v1.1
prunus_persica	Prupe.6G126600_v2.0.a1 Prupe.6G126700_v2.0.a1 Prupe.6G126700_v2.0.a1 Prupe.6G126800_v2.0.a1
pyrus_communis	pycom03g09610 pycom03g09620 pycom03g09650 pycom10g05210 pycom11g13330
rosa_chinensis	RchiOBHm_Chr3g0483921 RchiOBHm_Chr5g0043321 RchiOBHm_Chr5g0044031 RchiOBHm_Chr5g0044071 RchiOBHm_Chr5g0069061 RchiOBHm_Chr5g0069111 RchiOBHm_Chr5g0069131 RchiOBHm_Chr6g0256891 RchiOBHm_Chr6g0256901 RchiOBHm_Chr7g0198821 RchiOBHm_Chr7g0198851 RchiOBHm_Chr7g0198861 RchiOBHm_Chr7g0219381 RchiOBHm_Chr7g0230491
rosa_laevigata	RLG00000001491 RLG00000003837 RLG00000014654 RLG00000014655 RLG00000020958 RLG00000023252 RLG00000028966 RLG00000034179 RLG00000034211 RLG00000034236 RLG00000034238 RLG00000036034 RLG00000036038
rosa_multiflora	Rmu_co8020118.1_g000001 Rmu_sc0000060.1_g000002 Rmu_sc0000060.1_g000026 Rmu_sc0001157.1_g000010 Rmu_sc0001157.1_g000016 Rmu_sc0002252.1_g000009 Rmu_sc0003076.1_g000025 Rmu_sc0008623.1_g000018 Rmu_sc0008623.1_g000021 Rmu_sc0022203.1_g000001
rosa_roxburghii	Rroxscaffold_1G00036940 Rroxscaffold_1G00036990 Rroxscaffold_3G00230110 Rroxscaffold_3G00257340 Rroxscaffold_3G00257380 Rroxscaffold_6G00398020 Rroxscaffold_6G00398040 Rroxscaffold_7G00208240 Rroxscaffold_7G00208260
rosa_rugosa	Rorug01G0059500 Rorug03G0208700 Rorug03G0208700 Rorug03G0208800 Rorug03G0208900 Rorug03G0208900 Rorug04G0119300 Rorug05G0210300 Rorug05G0210300 Rorug05G0395800 Rorug05G0396000 Rorug05G0396100 Rorug05G0396200 Rorug05G0577200 Rorug07G0050100 Rorug07G0050200 Rorug07G0215200
rosa_samantha	Rh1BG061200 Rh2BG536700 Rh2DG545600 Rh3AG257500 Rh3BG294300 Rh3BG322600 Rh3CG292100 Rh3DG287300 Rh4BG178300 Rh5AG290800 Rh5AG291000 Rh5AG294200 Rh5AG294300 Rh5BG297400 Rh5BG302600 Rh5BG303000 Rh5CG329900 Rh5CG330100 Rh5DG307000 Rh5DG311500 Rh5DG311700 Rh6AG095800 Rh6BG086800 Rh6BG086900 Rh6CG082900 Rh6DG078600 Rh6DG078700 Rh7AG173700 Rh7AG174000 Rh7AG174100 Rh7AG327500 Rh7AG412100 Rh7BG177100 Rh7CG182900 Rh7CG183100 Rh7CG344800 Rh7CG429900 Rh7DG176600
rosa_wichuraiana	Rw0G003880 Rw0G006090 Rw2G043200 Rw3G023270 Rw3G023310 Rw5G026990 Rw5G027280 Rw5G027300 Rw5G042270 Rw6G008160 Rw7G015210 Rw7G015230

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	813
AccI	GTMKAC	1 cut(s)	295
AciI	CCGC	1 cut(s)	1282
AclI	AACGTT	1 cut(s)	1467
AclWI	GGATC	3 cut(s)	902, 915, 1397
AcoI	YGGCCR	1 cut(s)	704
AcsI	RAATTY	8 cut(s)	208, 232, 647, 952, 1057, 1173, 1220, 1389
AfaI	GTAC	4 cut(s)	771, 808, 1247, 1441
AfiI	CCNNNNNNNGG	2 cut(s)	827, 904
AflIII	ACRYGT	1 cut(s)	164
AgsI	TTSAA	7 cut(s)	430, 616, 839, 957, 1141, 1318, 1396
AhlI	ACTAGT	1 cut(s)	803
AjiI	CACGTC	1 cut(s)	167
AjnI	CCWGG	4 cut(s)	271, 576, 826, 1450
AleI	CACNNNNGTG	1 cut(s)	1274
AluBI	AGCT	9 cut(s)	124, 143, 199, 441, 1207, 1254, 1267, 1289, 1294
AluI	AGCT	9 cut(s)	124, 143, 199, 441, 1207, 1254, 1267, 1289, 1294
Alw26I	GTCTC	2 cut(s)	270, 848
AlwI	GGATC	3 cut(s)	902, 915, 1397
AoxI	GGCC	2 cut(s)	704, 1453
ApeKI	GCWGC	1 cut(s)	365
ApoI	RAATTY	8 cut(s)	208, 232, 647, 952, 1057, 1173, 1220, 1389
AseI	ATTAAT	1 cut(s)	1416
Asp700I	GAANNNNTTC	1 cut(s)	1136
AspS9I	GGNCC	2 cut(s)	325, 1017
AsuHPI	GGTGA	2 cut(s)	515, 1288
AvaII	GGWCC	2 cut(s)	325, 1017
BalI	TGGCCA	1 cut(s)	706
BamHI	GGATCC	1 cut(s)	907
BanII	GRGCYC	2 cut(s)	519, 680
BarI	GAAGNNNNNNTAC	2 cut(s)	976, 1008
BbsI	GAAGAC	1 cut(s)	1431
BbvI	GCAGC	1 cut(s)	377
BccI	CCATC	3 cut(s)	825, 1097, 1354
BciT130I	CCWGG	4 cut(s)	273, 578, 828, 1452
BciVI	GTATCC	1 cut(s)	866
BcoDI	GTCTC	2 cut(s)	270, 848
BcuI	ACTAGT	1 cut(s)	803
BfaI	CTAG	5 cut(s)	299, 464, 749, 804, 947
BfmI	CTRYAG	1 cut(s)	363
BfuI	GTATCC	1 cut(s)	866
BglII	AGATCT	1 cut(s)	155
BisI	GCNGC	1 cut(s)	366
BlpI	GCTNAGC	2 cut(s)	437, 1290
BlsI	GCNGC	1 cut(s)	367
BmcAI	AGTACT	1 cut(s)	808
Bme1390I	CCNGG	4 cut(s)	273, 578, 828, 1452
Bme18I	GGWCC	2 cut(s)	325, 1017
BmgBI	CACGTC	1 cut(s)	167
BmgT120I	GGNCC	2 cut(s)	325, 1017
BmiI	GGNNCC	5 cut(s)	58, 326, 819, 909, 1018
BmrFI	CCNGG	4 cut(s)	273, 578, 828, 1452
BmsI	GCATC	4 cut(s)	139, 1269, 1372, 1400
BpiI	GAAGAC	1 cut(s)	1431
BplI	GAGNNNNNCTC	2 cut(s)	518, 550
BpmI	CTGGAG	2 cut(s)	75, 898
Bpu1102I	GCTNAGC	2 cut(s)	437, 1290
BsaAI	YACGTR	1 cut(s)	714
BsaBI	GATNNNNATC	1 cut(s)	347
BsaI	GGTCTC	1 cut(s)	848
BsaJI	CCNNGG	1 cut(s)	1450
Bsc4I	CCNNNNNNNGG	2 cut(s)	827, 904
Bse118I	RCCGGY	1 cut(s)	1491
Bse1I	ACTGG	5 cut(s)	58, 67, 881, 935, 1228
Bse3DI	GCAATG	2 cut(s)	343, 738
Bse8I	GATNNNNATC	1 cut(s)	347
BseBI	CCWGG	4 cut(s)	273, 578, 828, 1452
BseDI	CCNNGG	1 cut(s)	1450
BseGI	GGATG	8 cut(s)	29, 351, 550, 669, 836, 1108, 1365, 1446
BseJI	GATNNNNATC	1 cut(s)	347
BseLI	CCNNNNNNNGG	2 cut(s)	827, 904
BseMI	GCAATG	2 cut(s)	343, 738
BseMII	CTCAG	5 cut(s)	89, 428, 859, 930, 1281
BseNI	ACTGG	5 cut(s)	58, 67, 881, 935, 1228
BseRI	GAGGAG	2 cut(s)	122, 1361
BseXI	GCAGC	1 cut(s)	377
BseYI	CCCAGC	1 cut(s)	512
BsgI	GTGCAG	1 cut(s)	1060
BshFI	GGCC	2 cut(s)	706, 1455
BsiSI	CCGG	1 cut(s)	1492
BslFI	GGGAC	2 cut(s)	338, 1030
BslI	CCNNNNNNNGG	2 cut(s)	827, 904
BsmAI	GTCTC	2 cut(s)	270, 848
BsmFI	GGGAC	2 cut(s)	338, 1030
BsmI	GAATGC	1 cut(s)	15
BsnI	GGCC	2 cut(s)	706, 1455
Bso31I	GGTCTC	1 cut(s)	848
Bsp1286I	GDGCHC	2 cut(s)	519, 680
Bsp143I	GATC	4 cut(s)	155, 183, 907, 1402
Bsp1720I	GCTNAGC	2 cut(s)	437, 1290
BspACI	CCGC	1 cut(s)	1282
BspANI	GGCC	2 cut(s)	706, 1455
BspCNI	CTCAG	5 cut(s)	90, 429, 858, 929, 1282
BspLI	GGNNCC	5 cut(s)	58, 326, 819, 909, 1018
BspMAI	CTGCAG	1 cut(s)	367
BspPI	GGATC	3 cut(s)	902, 915, 1397
BspQI	GCTCTTC	1 cut(s)	273
BspTNI	GGTCTC	1 cut(s)	848
BsrDI	GCAATG	2 cut(s)	343, 738
BsrFI	RCCGGY	1 cut(s)	1491
BsrI	ACTGG	5 cut(s)	58, 67, 881, 935, 1228
BssAI	RCCGGY	1 cut(s)	1491
BssECI	CCNNGG	1 cut(s)	1450
BssMI	GATC	4 cut(s)	155, 183, 907, 1402
Bst2UI	CCWGG	4 cut(s)	273, 578, 828, 1452
Bst4CI	ACNGT	3 cut(s)	43, 796, 853
Bst6I	CTCTTC	2 cut(s)	273, 1475
BstBAI	YACGTR	1 cut(s)	714
BstC8I	GCNNGC	4 cut(s)	68, 173, 704, 1493
BstDEI	CTNAG	8 cut(s)	46, 98, 320, 437, 690, 845, 916, 1290
BstF5I	GGATG	8 cut(s)	29, 351, 550, 669, 836, 1108, 1365, 1446
BstKTI	GATC	4 cut(s)	158, 186, 910, 1405
BstMAI	GTCTC	2 cut(s)	270, 848
BstMBI	GATC	4 cut(s)	155, 183, 907, 1402
BstMWI	GCNNNNNNNGC	2 cut(s)	149, 158
BstNI	CCWGG	4 cut(s)	273, 578, 828, 1452
BstNSI	RCATGY	4 cut(s)	175, 897, 946, 1171
BstSCI	CCNGG	4 cut(s)	271, 576, 826, 1450
BstSFI	CTRYAG	1 cut(s)	363
BstSNI	TACGTA	1 cut(s)	714
BstV1I	GCAGC	1 cut(s)	377
BstV2I	GAAGAC	1 cut(s)	1431
BstX2I	RGATCY	2 cut(s)	155, 907
BstYI	RGATCY	2 cut(s)	155, 907
BsuI	GTATCC	1 cut(s)	866
BsuRI	GGCC	2 cut(s)	706, 1455
BtrI	CACGTC	1 cut(s)	167
BtsCI	GGATG	8 cut(s)	29, 351, 550, 669, 836, 1108, 1365, 1446
BtsI	GCAGTG	1 cut(s)	1088
BtsIMutI	CAGTG	3 cut(s)	60, 924, 1088
Cac8I	GCNNGC	4 cut(s)	68, 173, 704, 1493
Cfr10I	RCCGGY	1 cut(s)	1491
Cfr13I	GGNCC	2 cut(s)	325, 1017
Csp6I	GTAC	4 cut(s)	770, 807, 1246, 1440
CviAII	CATG	6 cut(s)	172, 894, 943, 1010, 1168, 1183
CviQI	GTAC	4 cut(s)	770, 807, 1246, 1440
DdeI	CTNAG	8 cut(s)	46, 98, 320, 437, 690, 845, 916, 1290
DpnI	GATC	4 cut(s)	157, 185, 909, 1404
DpnII	GATC	4 cut(s)	155, 183, 907, 1402
EaeI	YGGCCR	1 cut(s)	704
Eam1104I	CTCTTC	2 cut(s)	273, 1475
EarI	CTCTTC	2 cut(s)	273, 1475
Eco105I	TACGTA	1 cut(s)	714
Eco24I	GRGCYC	2 cut(s)	519, 680
Eco31I	GGTCTC	1 cut(s)	848
Eco47I	GGWCC	2 cut(s)	325, 1017
EcoO109I	RGGNCCY	1 cut(s)	1017
EcoRII	CCWGG	4 cut(s)	271, 576, 826, 1450
EcoT22I	ATGCAT	1 cut(s)	1415
EcoT38I	GRGCYC	2 cut(s)	519, 680
FaeI	CATG	6 cut(s)	175, 897, 946, 1013, 1171, 1186
FalI	AAGNNNNNCTT	4 cut(s)	29, 61, 1310, 1342
FaqI	GGGAC	2 cut(s)	338, 1030
FatI	CATG	6 cut(s)	171, 893, 942, 1009, 1167, 1182
FblI	GTMKAC	1 cut(s)	295
Fnu4HI	GCNGC	1 cut(s)	366
FokI	GGATG	8 cut(s)	36, 338, 557, 676, 843, 1115, 1372, 1433
FriOI	GRGCYC	2 cut(s)	519, 680
Fsp4HI	GCNGC	1 cut(s)	366
FspBI	CTAG	5 cut(s)	299, 464, 749, 804, 947
GluI	GCNGC	1 cut(s)	366
GsaI	CCCAGC	1 cut(s)	516
GsuI	CTGGAG	2 cut(s)	75, 898
HaeIII	GGCC	2 cut(s)	706, 1455
HapII	CCGG	1 cut(s)	1492
Hin1II	CATG	6 cut(s)	175, 897, 946, 1013, 1171, 1186
HincII	GTYRAC	1 cut(s)	940
HindII	GTYRAC	1 cut(s)	940
HinfI	GANTC	5 cut(s)	239, 835, 931, 999, 1483
HpaII	CCGG	1 cut(s)	1492
HphI	GGTGA	2 cut(s)	515, 1288
Hpy166II	GTNNAC	6 cut(s)	296, 478, 723, 770, 940, 1246
Hpy188I	TCNGA	9 cut(s)	148, 206, 253, 574, 643, 848, 1238, 1263, 1345
Hpy188III	TCNNGA	6 cut(s)	404, 749, 996, 1117, 1141, 1459
Hpy8I	GTNNAC	6 cut(s)	296, 478, 723, 770, 940, 1246
Hpy99I	CGWCG	1 cut(s)	171
HpyAV	CCTTC	2 cut(s)	661, 1350
HpyCH4III	ACNGT	3 cut(s)	43, 796, 853
HpyCH4IV	ACGT	7 cut(s)	166, 408, 713, 787, 1248, 1431, 1467
HpyCH4V	TGCA	8 cut(s)	175, 230, 336, 365, 446, 1077, 1171, 1413
HpyF10VI	GCNNNNNNNGC	2 cut(s)	149, 158
HpyF3I	CTNAG	8 cut(s)	46, 98, 320, 437, 690, 845, 916, 1290
HpySE526I	ACGT	7 cut(s)	166, 408, 713, 787, 1248, 1431, 1467
Hsp92II	CATG	6 cut(s)	175, 897, 946, 1013, 1171, 1186
KroI	GCCGGC	1 cut(s)	1491
KroNI	GCCGGC	1 cut(s)	1493
Kzo9I	GATC	4 cut(s)	155, 183, 907, 1402
LguI	GCTCTTC	1 cut(s)	273
LmnI	GCTCC	3 cut(s)	56, 817, 1212
Lsp1109I	GCAGC	1 cut(s)	377
LweI	GCATC	4 cut(s)	139, 1269, 1372, 1400
MaeI	CTAG	5 cut(s)	299, 464, 749, 804, 947
MaeII	ACGT	7 cut(s)	166, 408, 713, 787, 1248, 1431, 1467
MaeIII	GTNAC	3 cut(s)	49, 799, 1484
MalI	GATC	4 cut(s)	157, 185, 909, 1404
MboI	GATC	4 cut(s)	155, 183, 907, 1402
MboII	GAAGA	6 cut(s)	229, 260, 984, 1301, 1436, 1492
MfeI	CAATTG	1 cut(s)	329
MflI	RGATCY	2 cut(s)	155, 907
MhlI	GDGCHC	2 cut(s)	519, 680
MlsI	TGGCCA	1 cut(s)	706
MluNI	TGGCCA	1 cut(s)	706
MlyI	GAGTC	2 cut(s)	233, 1492
MmeI	TCCRAC	2 cut(s)	597, 815
Mox20I	TGGCCA	1 cut(s)	706
Mph1103I	ATGCAT	1 cut(s)	1415
MroNI	GCCGGC	1 cut(s)	1491
MroXI	GAANNNNTTC	1 cut(s)	1136
MscI	TGGCCA	1 cut(s)	706
MseI	TTAA	7 cut(s)	212, 357, 411, 458, 539, 1025, 1416
MslI	CAYNNNNRTG	2 cut(s)	1101, 1274
Msp20I	TGGCCA	1 cut(s)	706
MspI	CCGG	1 cut(s)	1492
MspR9I	CCNGG	4 cut(s)	273, 578, 828, 1452
MunI	CAATTG	1 cut(s)	329
Mva1269I	GAATGC	1 cut(s)	15
MvaI	CCWGG	4 cut(s)	273, 578, 828, 1452
MwoI	GCNNNNNNNGC	2 cut(s)	149, 158
NaeI	GCCGGC	1 cut(s)	1493
NdeII	GATC	4 cut(s)	155, 183, 907, 1402
NgoMIV	GCCGGC	1 cut(s)	1491
NlaIII	CATG	6 cut(s)	175, 897, 946, 1013, 1171, 1186
NlaIV	GGNNCC	5 cut(s)	58, 326, 819, 909, 1018
NmuCI	GTSAC	1 cut(s)	1484
NsiI	ATGCAT	1 cut(s)	1415
NspI	RCATGY	4 cut(s)	175, 897, 946, 1171
OliI	CACNNNNGTG	1 cut(s)	1274
PaeI	GCATGC	1 cut(s)	175
PciSI	GCTCTTC	1 cut(s)	273
PctI	GAATGC	1 cut(s)	15
PdiI	GCCGGC	1 cut(s)	1493
PdmI	GAANNNNTTC	1 cut(s)	1136
PfeI	GAWTC	3 cut(s)	835, 931, 999
PfoI	TCCNGGA	2 cut(s)	271, 826
PkrI	GCNGC	1 cut(s)	367
PleI	GAGTC	2 cut(s)	233, 1491
PpsI	GAGTC	2 cut(s)	233, 1491
Ppu21I	YACGTR	1 cut(s)	714
PpuMI	RGGWCCY	1 cut(s)	1017
PshBI	ATTAAT	1 cut(s)	1416
PsiI	TTATAA	1 cut(s)	813
Psp1406I	AACGTT	1 cut(s)	1467
Psp5II	RGGWCCY	1 cut(s)	1017
Psp6I	CCWGG	4 cut(s)	271, 576, 826, 1450
PspFI	CCCAGC	1 cut(s)	512
PspGI	CCWGG	4 cut(s)	271, 576, 826, 1450
PspN4I	GGNNCC	5 cut(s)	58, 326, 819, 909, 1018
PspPI	GGNCC	2 cut(s)	325, 1017
PspPPI	RGGWCCY	1 cut(s)	1017
PstI	CTGCAG	1 cut(s)	367
PsuI	RGATCY	2 cut(s)	155, 907
RsaI	GTAC	4 cut(s)	771, 808, 1247, 1441
RsaNI	GTAC	4 cut(s)	770, 807, 1246, 1440
RseI	CAYNNNNRTG	2 cut(s)	1101, 1274
SapI	GCTCTTC	1 cut(s)	273
SaqAI	TTAA	7 cut(s)	212, 357, 411, 458, 539, 1025, 1416
SatI	GCNGC	1 cut(s)	366
Sau3AI	GATC	4 cut(s)	155, 183, 907, 1402
Sau96I	GGNCC	2 cut(s)	325, 1017
ScaI	AGTACT	1 cut(s)	808
SchI	GAGTC	2 cut(s)	233, 1492
ScrFI	CCNGG	4 cut(s)	273, 578, 828, 1452
SduI	GDGCHC	2 cut(s)	519, 680
SfaNI	GCATC	4 cut(s)	139, 1269, 1372, 1400
SfcI	CTRYAG	1 cut(s)	363
SinI	GGWCC	2 cut(s)	325, 1017
SmiMI	CAYNNNNRTG	2 cut(s)	1101, 1274
SnaBI	TACGTA	1 cut(s)	714
SpeI	ACTAGT	1 cut(s)	803
SphI	GCATGC	1 cut(s)	175
SsiI	CCGC	1 cut(s)	1282
SspMI	CTAG	5 cut(s)	299, 464, 749, 804, 947
StyD4I	CCNGG	4 cut(s)	271, 576, 826, 1450
TaaI	ACNGT	3 cut(s)	43, 796, 853
TaiI	ACGT	7 cut(s)	169, 411, 716, 790, 1251, 1434, 1470
TaqI	TCGA	3 cut(s)	19, 525, 758
TatI	WGTACW	1 cut(s)	806
TfiI	GAWTC	3 cut(s)	835, 931, 999
Tru1I	TTAA	7 cut(s)	212, 357, 411, 458, 539, 1025, 1416
Tru9I	TTAA	7 cut(s)	212, 357, 411, 458, 539, 1025, 1416
TscAI	CASTG	3 cut(s)	67, 924, 1095
TseFI	GTSAC	1 cut(s)	1484
TseI	GCWGC	1 cut(s)	365
Tsp45I	GTSAC	1 cut(s)	1484
TspDTI	ATGAA	7 cut(s)	640, 680, 992, 1070, 1085, 1171, 1314
TspRI	CASTG	3 cut(s)	67, 924, 1095
VpaK11BI	GGWCC	2 cut(s)	325, 1017
VspI	ATTAAT	1 cut(s)	1416
XapI	RAATTY	8 cut(s)	208, 232, 647, 952, 1057, 1173, 1220, 1389
XbaI	TCTAGA	1 cut(s)	748
XceI	RCATGY	4 cut(s)	175, 897, 946, 1171
XcmI	CCANNNNNNNNNTGG	1 cut(s)	828
XmiI	GTMKAC	1 cut(s)	295
XmnI	GAANNNNTTC	1 cut(s)	1136
XspI	CTAG	5 cut(s)	299, 464, 749, 804, 947
ZrmI	AGTACT	1 cut(s)	808
Zsp2I	ATGCAT	1 cut(s)	1415

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.