FvH4_3g00833

Catalyzes xyloglucan endohydrolysis (XEH) and or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0000460
Physical Location & Seq
Forward (+)
429630 .. 431126
1497 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g00833.t1

Sequence Viewer

Length: 876 bp
ATGGCTAGCAGATACTGCCTGGTGTTTTCGATTGCTTTTCTCATTGGTTCTTTGATGGCTGTTATCTCTTCTGCTAGTAATTTCAATCAGGATTTTGAGATTACATGGGGTGATGGCCGTGCCAAGATACTAAACAACAACGAGCTTCTTGTACTCTTCCTCGATAGAATCTCAGGGTCAGGATTCCAGTCCAAGTATGAGTATCTATTTGGTAAGATTGATATGCAGCTCAAGCTTGTCCCTGGAAACTCTGCTGGCACTGTCACCGCCTATTATTTGTCCTCAAAAGGGTCAGCATGGGATGAGATAGATTTCGAGTTCTTGGGTAACTTGAGTGGTGATCCGTACACCATCCACACCAATGTGTATAGCCAAGGCAAGGGCGATAGAGAGCAACAATTCCATCTTTGGTTTGACCCAACTACCGACTTTCATACATATTCCATTCTTTGGAACCCTCGGAGCATTGTATTTTATGTTGATGGCACCCCCATAAGAGAGTTCAAGAACTTGGAGTCAATTGGTGTTCCCTTCCCAAAGGACCAGCCCATGAGATTACACTCTAGCCTTTGGAGTGCAGATGACTGGGCTACAAGGGGTGGTCTGGTTAAAACAGACTGGACAAAAGCACCTTTCATTGCTTCTTATAGGAACTTCAACGCCAATGCCTGTATATGGTCCTCTGGAACATCTTCCTGCACTTCACCATCCACTAAAGGTGAATGGCTCTCACAAGAAATGGATTTAGCAAGTAAGCAAAAATTGAAAATGGTGCAAGAGAAGTTCATGATCTACAATTACTGCACAGACACAAAGAGGTTTCCCCAAGGTTTTCCTAAAGAATGCAGCAGGGTATCCGAGGAAATCCGAAAATAA

Protein Analysis

292

Amino Acids

33.12

Weight (kDa)

6.44

Isoelectric Point (pI)

37.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_16 PF00722 28 - 209 6.6e-66 Glycosyl hydrolases family 16
XET_C PF06955 238 - 282 1.9e-18 Xyloglucan endo-transglycosylase (XET) C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000460)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G25810 AT4G30270 AT5G57550 AT5G57560
fragaria_vesca FvH4_3g00833 FvH4_3g00840 FvH4_3g00850 FvH4_4g06700 FvH4_4g09230
malus_domestica MD13G1237300.v1.1 MD13G1268000.v1.1 MD13G1268900.v1.1 MD16G1267200.v1.1 MD16G1267300.v1.1
prunus_persica Prupe.1G069800_v2.0.a1 Prupe.1G088600_v2.0.a1 Prupe.1G088800_v2.0.a1 Prupe.1G088900_v2.0.a1 Prupe.1G089000_v2.0.a1
pyrus_communis pycom13g21020 pycom13g22350 pycom13g22380 pycom16g23630 pycom16g23640
rosa_chinensis RchiOBHm_Chr4g0398631 RchiOBHm_Chr4g0402891 RchiOBHm_Chr4g0402911 RchiOBHm_Chr4g0402921 RchiOBHm_Chr4g0402931 RchiOBHm_Chr4g0402951 RchiOBHm_Chr4g0402961 RchiOBHm_Chr4g0402981
rosa_laevigata RLG00000009031 RLG00000009033 RLG00000009034 RLG00000009035 RLG00000009039 RLG00000009040 RLG00000009339
rosa_multiflora Rmu_co8186292.1_g000001 Rmu_sc0000990.1_g000004 Rmu_sc0001023.1_g000002 Rmu_sc0001023.1_g000004 Rmu_sc0001023.1_g000007 Rmu_sc0001023.1_g000015 Rmu_sc0002178.1_g000007 Rmu_sc0002451.1_g000069 Rmu_sc0002451.1_g000079 Rmu_sc0002451.1_g000080 Rmu_sc0008058.1_g000026 Rmu_sc0008058.1_g000029 Rmu_sc0008058.1_g000030
rosa_roxburghii Rroxscaffold_5G00343590 Rroxscaffold_5G00347620 Rroxscaffold_5G00347630 Rroxscaffold_5G00347640 Rroxscaffold_5G00347650 Rroxscaffold_5G00347660 Rroxscaffold_5G00347670 Rroxscaffold_5G00347690
rosa_rugosa Rorug04G0009900 Rorug04G0039000 Rorug04G0039100 Rorug04G0039200 Rorug04G0039300 Rorug04G0039400 Rorug04G0039500 Rorug04G0039600 Rorug04G0039600 Rorug04G0040200
rosa_samantha Rh4AG087800 Rh4AG116500 Rh4AG116700 Rh4AG116800 Rh4AG116900 Rh4AG117000 Rh4AG117100 Rh4BG109300 Rh4BG109400 Rh4BG109500 Rh4BG109600 Rh4BG109700 Rh4BG109800 Rh4CG096100 Rh4CG123700 Rh4CG124200
rosa_wichuraiana Rw4G007280 Rw4G009320 Rw4G009340 Rw4G009370 Rw4G009380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 485
AccB7I CCANNNNNTGG 1 cut(s) 450
AciI CCGC 1 cut(s) 267
AclWI GGATC 1 cut(s) 335
AcoI YGGCCR 1 cut(s) 115
AfaI GTAC 2 cut(s) 153, 347
AfiI CCNNNNNNNGG 4 cut(s) 288, 379, 450, 675
AgsI TTSAA 4 cut(s) 85, 505, 658, 766
AjnI CCWGG 2 cut(s) 18, 241
AleI CACNNNNGTG 1 cut(s) 362
AluBI AGCT 3 cut(s) 145, 229, 235
AluI AGCT 3 cut(s) 145, 229, 235
AlwI GGATC 1 cut(s) 335
AlwNI CAGNNNCTG 1 cut(s) 15
AoxI GGCC 1 cut(s) 115
ApeKI GCWGC 2 cut(s) 226, 846
Asp700I GAANNNNTTC 1 cut(s) 691
AspS9I GGNCC 2 cut(s) 541, 678
AsuHPI GGTGA 5 cut(s) 122, 256, 350, 696, 731
AsuNHI GCTAGC 1 cut(s) 5
AvaII GGWCC 2 cut(s) 541, 678
BanI GGYRCC 1 cut(s) 485
BbvI GCAGC 2 cut(s) 238, 858
BccI CCATC 6 cut(s) 49, 107, 359, 411, 476, 715
BceAI ACGGC 1 cut(s) 102
BciT130I CCWGG 2 cut(s) 20, 243
BciVI GTATCC 1 cut(s) 865
BfaI CTAG 3 cut(s) 6, 75, 564
BfuI GTATCC 1 cut(s) 865
BisI GCNGC 2 cut(s) 227, 847
BlsI GCNGC 2 cut(s) 228, 848
Bme1390I CCNGG 2 cut(s) 20, 243
Bme18I GGWCC 2 cut(s) 541, 678
BmgT120I GGNCC 2 cut(s) 541, 678
BmiI GGNNCC 2 cut(s) 455, 487
BmrFI CCNGG 2 cut(s) 20, 243
BmrI ACTGGG 1 cut(s) 595
BmtI GCTAGC 1 cut(s) 9
BmuI ACTGGG 1 cut(s) 595
BpuEI CTTGAG 2 cut(s) 215, 352
BsaJI CCNNGG 5 cut(s) 241, 373, 458, 826, 858
Bsc4I CCNNNNNNNGG 4 cut(s) 288, 379, 450, 675
Bse1I ACTGG 3 cut(s) 187, 590, 623
Bse3DI GCAATG 1 cut(s) 636
BseBI CCWGG 2 cut(s) 20, 243
BseDI CCNNGG 5 cut(s) 241, 373, 458, 826, 858
BseGI GGATG 3 cut(s) 307, 351, 707
BseLI CCNNNNNNNGG 4 cut(s) 288, 379, 450, 675
BseMI GCAATG 1 cut(s) 636
BseMII CTCAG 1 cut(s) 186
BseNI ACTGG 3 cut(s) 187, 590, 623
BseXI GCAGC 2 cut(s) 238, 858
BsgI GTGCAG 3 cut(s) 597, 682, 787
BshFI GGCC 1 cut(s) 117
BshNI GGYRCC 1 cut(s) 485
BslFI GGGAC 1 cut(s) 224
BslI CCNNNNNNNGG 4 cut(s) 288, 379, 450, 675
BsmFI GGGAC 1 cut(s) 224
BsmI GAATGC 1 cut(s) 848
BsnI GGCC 1 cut(s) 117
Bsp143I GATC 2 cut(s) 340, 789
BspACI CCGC 1 cut(s) 267
BspANI GGCC 1 cut(s) 117
BspCNI CTCAG 1 cut(s) 185
BspHI TCATGA 1 cut(s) 786
BspLI GGNNCC 2 cut(s) 455, 487
BspOI GCTAGC 1 cut(s) 9
BspPI GGATC 1 cut(s) 335
BspT107I GGYRCC 1 cut(s) 485
BsrDI GCAATG 1 cut(s) 636
BsrI ACTGG 3 cut(s) 187, 590, 623
BssECI CCNNGG 5 cut(s) 241, 373, 458, 826, 858
BssMI GATC 2 cut(s) 340, 789
BssT1I CCWWGG 2 cut(s) 373, 826
Bst2UI CCWGG 2 cut(s) 20, 243
Bst4CI ACNGT 1 cut(s) 262
Bst6I CTCTTC 2 cut(s) 73, 161
BstAPI GCANNNNNTGC 1 cut(s) 15
BstC8I GCNNGC 2 cut(s) 7, 256
BstDEI CTNAG 1 cut(s) 172
BstF5I GGATG 3 cut(s) 307, 351, 707
BstKTI GATC 2 cut(s) 343, 792
BstMBI GATC 2 cut(s) 340, 789
BstMWI GCNNNNNNNGC 2 cut(s) 15, 232
BstNI CCWGG 2 cut(s) 20, 243
BstSCI CCNGG 2 cut(s) 18, 241
BstV1I GCAGC 2 cut(s) 238, 858
BsuI GTATCC 1 cut(s) 865
BsuRI GGCC 1 cut(s) 117
BtsCI GGATG 3 cut(s) 307, 351, 707
BtsIMutI CAGTG 1 cut(s) 258
Cac8I GCNNGC 2 cut(s) 7, 256
CaiI CAGNNNCTG 1 cut(s) 15
CciI TCATGA 1 cut(s) 786
Cfr13I GGNCC 2 cut(s) 541, 678
Csp6I GTAC 2 cut(s) 152, 346
CviAII CATG 4 cut(s) 105, 297, 550, 787
CviQI GTAC 2 cut(s) 152, 346
DdeI CTNAG 1 cut(s) 172
DpnI GATC 2 cut(s) 342, 791
DpnII GATC 2 cut(s) 340, 789
EaeI YGGCCR 1 cut(s) 115
Eam1104I CTCTTC 2 cut(s) 73, 161
EarI CTCTTC 2 cut(s) 73, 161
Eco130I CCWWGG 2 cut(s) 373, 826
Eco47I GGWCC 2 cut(s) 541, 678
EcoRII CCWGG 2 cut(s) 18, 241
EcoT14I CCWWGG 2 cut(s) 373, 826
ErhI CCWWGG 2 cut(s) 373, 826
FaeI CATG 4 cut(s) 108, 300, 553, 790
FaqI GGGAC 1 cut(s) 224
FatI CATG 4 cut(s) 104, 296, 549, 786
Fnu4HI GCNGC 2 cut(s) 227, 847
FokI GGATG 3 cut(s) 314, 338, 694
Fsp4HI GCNGC 2 cut(s) 227, 847
FspBI CTAG 3 cut(s) 6, 75, 564
GluI GCNGC 2 cut(s) 227, 847
HaeIII GGCC 1 cut(s) 117
Hin1II CATG 4 cut(s) 108, 300, 553, 790
HindIII AAGCTT 1 cut(s) 233
HinfI GANTC 3 cut(s) 168, 183, 515
HphI GGTGA 5 cut(s) 122, 256, 350, 696, 731
Hpy166II GTNNAC 1 cut(s) 348
Hpy188I TCNGA 3 cut(s) 462, 859, 869
Hpy188III TCNNGA 5 cut(s) 89, 180, 505, 684, 787
Hpy8I GTNNAC 1 cut(s) 348
HpyAV CCTTC 1 cut(s) 541
HpyCH4III ACNGT 1 cut(s) 262
HpyCH4V TGCA 6 cut(s) 226, 578, 699, 775, 804, 846
HpyF10VI GCNNNNNNNGC 2 cut(s) 15, 232
HpyF3I CTNAG 1 cut(s) 172
Hsp92II CATG 4 cut(s) 108, 300, 553, 790
Kzo9I GATC 2 cut(s) 340, 789
LmnI GCTCC 1 cut(s) 462
Lsp1109I GCAGC 2 cut(s) 238, 858
MaeI CTAG 3 cut(s) 6, 75, 564
MaeIII GTNAC 2 cut(s) 262, 326
MalI GATC 2 cut(s) 342, 791
MboI GATC 2 cut(s) 340, 789
MboII GAAGA 3 cut(s) 60, 148, 684
MfeI CAATTG 1 cut(s) 519
MluCI AATT 5 cut(s) 79, 398, 519, 761, 796
MlyI GAGTC 1 cut(s) 524
MnlI CCTC 6 cut(s) 170, 292, 468, 691, 810, 853
MroXI GAANNNNTTC 1 cut(s) 691
MseI TTAA 1 cut(s) 609
MslI CAYNNNNRTG 2 cut(s) 360, 362
MspR9I CCNGG 2 cut(s) 20, 243
MunI CAATTG 1 cut(s) 519
Mva1269I GAATGC 1 cut(s) 848
MvaI CCWGG 2 cut(s) 20, 243
MwoI GCNNNNNNNGC 2 cut(s) 15, 232
NdeII GATC 2 cut(s) 340, 789
NheI GCTAGC 1 cut(s) 5
NlaIII CATG 4 cut(s) 108, 300, 553, 790
NlaIV GGNNCC 2 cut(s) 455, 487
NmuCI GTSAC 1 cut(s) 262
OliI CACNNNNGTG 1 cut(s) 362
PagI TCATGA 1 cut(s) 786
PctI GAATGC 1 cut(s) 848
PdmI GAANNNNTTC 1 cut(s) 691
PfeI GAWTC 2 cut(s) 168, 183
PflMI CCANNNNNTGG 1 cut(s) 450
PkrI GCNGC 2 cut(s) 228, 848
PleI GAGTC 1 cut(s) 523
PpsI GAGTC 1 cut(s) 523
Psp6I CCWGG 2 cut(s) 18, 241
PspGI CCWGG 2 cut(s) 18, 241
PspN4I GGNNCC 2 cut(s) 455, 487
PspPI GGNCC 2 cut(s) 541, 678
PstNI CAGNNNCTG 1 cut(s) 15
RsaI GTAC 2 cut(s) 153, 347
RsaNI GTAC 2 cut(s) 152, 346
RseI CAYNNNNRTG 2 cut(s) 360, 362
SaqAI TTAA 1 cut(s) 609
SatI GCNGC 2 cut(s) 227, 847
Sau3AI GATC 2 cut(s) 340, 789
Sau96I GGNCC 2 cut(s) 541, 678
SchI GAGTC 1 cut(s) 524
ScrFI CCNGG 2 cut(s) 20, 243
SetI ASST 7 cut(s) 147, 231, 237, 634, 721, 821, 832
SinI GGWCC 2 cut(s) 541, 678
SmiMI CAYNNNNRTG 2 cut(s) 360, 362
SmlI CTYRAG 2 cut(s) 230, 331
SmoI CTYRAG 2 cut(s) 230, 331
Sse9I AATT 5 cut(s) 79, 398, 519, 761, 796
SsiI CCGC 1 cut(s) 267
SspMI CTAG 3 cut(s) 6, 75, 564
StyD4I CCNGG 2 cut(s) 18, 241
StyI CCWWGG 2 cut(s) 373, 826
TaaI ACNGT 1 cut(s) 262
TaqI TCGA 3 cut(s) 29, 162, 315
TasI AATT 5 cut(s) 79, 398, 519, 761, 796
TatI WGTACW 1 cut(s) 151
TfiI GAWTC 2 cut(s) 168, 183
Tru1I TTAA 1 cut(s) 609
Tru9I TTAA 1 cut(s) 609
TscAI CASTG 1 cut(s) 265
TseFI GTSAC 1 cut(s) 262
TseI GCWGC 2 cut(s) 226, 846
Tsp45I GTSAC 1 cut(s) 262
TspDTI ATGAA 3 cut(s) 422, 625, 775
TspGWI ACGGA 1 cut(s) 333
TspRI CASTG 1 cut(s) 265
Van91I CCANNNNNTGG 1 cut(s) 450
VpaK11BI GGWCC 2 cut(s) 541, 678
XmnI GAANNNNTTC 1 cut(s) 691
XspI CTAG 3 cut(s) 6, 75, 564
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.