MD13G1268000.v1.1

Catalyzes xyloglucan endohydrolysis (XEH) and or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0000460
Physical Location & Seq
Reverse (-)
35176554 .. 35177848
1295 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1268000.v1.1.491

Sequence Viewer

Length: 870 bp
ATGGCTTCCTTCAAATCACTTATTTCATTATTAACGCTGCTGACTCCTCTTGCTATAAGCTCTTTAATATTTGCCTCTGCTGGTAACTTTAACCAGGAATTTGAGATTACATGGGGAGACGGTCGAGCTAAGATACTCAACAATGGCGAGCTTCTCATTCTTTCACTTGACATAGTCTCTGGCTCTGCATTCGAATCCAAAAATGAGTATTTGTTCGGCAAGATTGACATGCAGATCAAGCTTGTCCCTGGAAACTCTGCTGGCACTGTCACAGCCTACTATTTATCATCAAAAGGATCAGCATGGGATGAGATAGATTTTGAGTTCTTGGGTAACTTGACTGGAGACCCTTACATTCTTCACACCAATGTCTTTAGCCAAGGCAAAGGCAACAGAGAGCAAAAATTCTACCTCTGGTTTGACCCAACGGCTGACTTTCACACCTATTCCATCCTCTGGAATCCACATTGCATAATTTTCTCTGTGGATGGTACTCCAATTAGAGAGTTCAAGAACTTGGAATCAATTGGTGTTCCTTTTCCAAAGCACCAACCAATGAGGATACACTCTAGCCTCTGGAATGCGGATGATTGGGCCACAAGGGGTGGACTTATCAAAACAGATTGGAGTAGTGCTCCTTTCACTGCTTCATACAGGAATTTCACTGCCGATAACGCTTGTGTTTGGTCATCAAATGGAGAATCTTCATGCAAATCTTCACCATCACAAAGTAATGCATGGCTATCAGAAGAGCTCGATTCAATTAGGAAGAAGAGGCTTAGCTGGGTCCAAAACAACTACATGATCTATAATTACTGCACTGACGGAAAGCGATTTCCCCAGGGTTTCCCTGTTGAATGCAAATCTTAA

Protein Analysis

290

Amino Acids

32.49

Weight (kDa)

5.76

Isoelectric Point (pI)

37.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_16 PF00722 31 - 210 1.1e-64 Glycosyl hydrolases family 16
XET_C PF06955 243 - 287 9.3e-20 Xyloglucan endo-transglycosylase (XET) C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000460)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G25810 AT4G30270 AT5G57550 AT5G57560
fragaria_vesca FvH4_3g00833 FvH4_3g00840 FvH4_3g00850 FvH4_4g06700 FvH4_4g09230
malus_domestica MD13G1237300.v1.1 MD13G1268000.v1.1 MD13G1268900.v1.1 MD16G1267200.v1.1 MD16G1267300.v1.1
prunus_persica Prupe.1G069800_v2.0.a1 Prupe.1G088600_v2.0.a1 Prupe.1G088800_v2.0.a1 Prupe.1G088900_v2.0.a1 Prupe.1G089000_v2.0.a1
pyrus_communis pycom13g21020 pycom13g22350 pycom13g22380 pycom16g23630 pycom16g23640
rosa_chinensis RchiOBHm_Chr4g0398631 RchiOBHm_Chr4g0402891 RchiOBHm_Chr4g0402911 RchiOBHm_Chr4g0402921 RchiOBHm_Chr4g0402931 RchiOBHm_Chr4g0402951 RchiOBHm_Chr4g0402961 RchiOBHm_Chr4g0402981
rosa_laevigata RLG00000009031 RLG00000009033 RLG00000009034 RLG00000009035 RLG00000009039 RLG00000009040 RLG00000009339
rosa_multiflora Rmu_co8186292.1_g000001 Rmu_sc0000990.1_g000004 Rmu_sc0001023.1_g000002 Rmu_sc0001023.1_g000004 Rmu_sc0001023.1_g000007 Rmu_sc0001023.1_g000015 Rmu_sc0002178.1_g000007 Rmu_sc0002451.1_g000069 Rmu_sc0002451.1_g000079 Rmu_sc0002451.1_g000080 Rmu_sc0008058.1_g000026 Rmu_sc0008058.1_g000029 Rmu_sc0008058.1_g000030
rosa_roxburghii Rroxscaffold_5G00343590 Rroxscaffold_5G00347620 Rroxscaffold_5G00347630 Rroxscaffold_5G00347640 Rroxscaffold_5G00347650 Rroxscaffold_5G00347660 Rroxscaffold_5G00347670 Rroxscaffold_5G00347690
rosa_rugosa Rorug04G0009900 Rorug04G0039000 Rorug04G0039100 Rorug04G0039200 Rorug04G0039300 Rorug04G0039400 Rorug04G0039500 Rorug04G0039600 Rorug04G0039600 Rorug04G0040200
rosa_samantha Rh4AG087800 Rh4AG116500 Rh4AG116700 Rh4AG116800 Rh4AG116900 Rh4AG117000 Rh4AG117100 Rh4BG109300 Rh4BG109400 Rh4BG109500 Rh4BG109600 Rh4BG109700 Rh4BG109800 Rh4CG096100 Rh4CG123700 Rh4CG124200
rosa_wichuraiana Rw4G007280 Rw4G009320 Rw4G009340 Rw4G009370 Rw4G009380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 456
AciI CCGC 1 cut(s) 584
AclWI GGATC 1 cut(s) 304
AcsI RAATTY 3 cut(s) 98, 404, 658
AfaI GTAC 1 cut(s) 493
AfiI CCNNNNNNNGG 1 cut(s) 456
AgsI TTSAA 4 cut(s) 13, 511, 762, 857
AjnI CCWGG 3 cut(s) 93, 247, 840
AluBI AGCT 6 cut(s) 60, 128, 151, 241, 754, 783
AluI AGCT 6 cut(s) 60, 128, 151, 241, 754, 783
Alw21I GWGCWC 2 cut(s) 637, 756
Alw26I GTCTC 3 cut(s) 111, 181, 339
AlwI GGATC 1 cut(s) 304
AoxI GGCC 1 cut(s) 594
ApeKI GCWGC 1 cut(s) 37
ApoI RAATTY 3 cut(s) 98, 404, 658
AspS9I GGNCC 2 cut(s) 594, 787
AsuHPI GGTGA 1 cut(s) 711
AsuII TTCGAA 1 cut(s) 192
AvaII GGWCC 1 cut(s) 787
BanII GRGCYC 1 cut(s) 756
Bbv12I GWGCWC 2 cut(s) 637, 756
BbvI GCAGC 1 cut(s) 24
BccI CCATC 3 cut(s) 458, 482, 730
BceAI ACGGC 1 cut(s) 444
BciT130I CCWGG 3 cut(s) 95, 249, 842
BciVI GTATCC 1 cut(s) 555
BcoDI GTCTC 3 cut(s) 111, 181, 339
BfaI CTAG 1 cut(s) 570
BfuI GTATCC 1 cut(s) 555
BisI GCNGC 1 cut(s) 38
BlpI GCTNAGC 1 cut(s) 779
BlsI GCNGC 1 cut(s) 39
Bme1390I CCNGG 3 cut(s) 95, 249, 842
Bme18I GGWCC 1 cut(s) 787
BmgT120I GGNCC 2 cut(s) 594, 787
BmiI GGNNCC 1 cut(s) 788
BmrFI CCNGG 3 cut(s) 95, 249, 842
BplI GAGNNNNNCTC 2 cut(s) 619, 651
BpmI CTGGAG 1 cut(s) 363
Bpu1102I GCTNAGC 1 cut(s) 779
Bpu14I TTCGAA 1 cut(s) 192
BsaI GGTCTC 1 cut(s) 339
BsaJI CCNNGG 4 cut(s) 247, 379, 840, 841
Bsc4I CCNNNNNNNGG 1 cut(s) 456
Bse1I ACTGG 1 cut(s) 346
Bse3DI GCAATG 1 cut(s) 466
BseBI CCWGG 3 cut(s) 95, 249, 842
BseDI CCNNGG 4 cut(s) 247, 379, 840, 841
BseGI GGATG 4 cut(s) 313, 450, 493, 592
BseLI CCNNNNNNNGG 1 cut(s) 456
BseMI GCAATG 1 cut(s) 466
BseNI ACTGG 1 cut(s) 346
BseRI GAGGAG 1 cut(s) 36
BseXI GCAGC 1 cut(s) 24
BseYI CCCAGC 1 cut(s) 783
BsgI GTGCAG 1 cut(s) 802
Bsh1285I CGRYCG 1 cut(s) 124
BshFI GGCC 1 cut(s) 596
BsiEI CGRYCG 1 cut(s) 124
BsiHKAI GWGCWC 2 cut(s) 637, 756
BslFI GGGAC 1 cut(s) 230
BslI CCNNNNNNNGG 1 cut(s) 456
BsmAI GTCTC 3 cut(s) 111, 181, 339
BsmBI CGTCTC 1 cut(s) 111
BsmFI GGGAC 1 cut(s) 230
BsmI GAATGC 3 cut(s) 188, 586, 863
BsnI GGCC 1 cut(s) 596
Bso31I GGTCTC 1 cut(s) 339
Bsp119I TTCGAA 1 cut(s) 192
Bsp1286I GDGCHC 2 cut(s) 637, 756
Bsp143I GATC 3 cut(s) 234, 296, 804
Bsp1720I GCTNAGC 1 cut(s) 779
BspACI CCGC 1 cut(s) 584
BspANI GGCC 1 cut(s) 596
BspLI GGNNCC 1 cut(s) 788
BspPI GGATC 1 cut(s) 304
BspQI GCTCTTC 1 cut(s) 744
BspT104I TTCGAA 1 cut(s) 192
BspTNI GGTCTC 1 cut(s) 339
BsrDI GCAATG 1 cut(s) 466
BsrI ACTGG 1 cut(s) 346
BssECI CCNNGG 4 cut(s) 247, 379, 840, 841
BssMI GATC 3 cut(s) 234, 296, 804
BssT1I CCWWGG 1 cut(s) 379
Bst2UI CCWGG 3 cut(s) 95, 249, 842
Bst4CI ACNGT 2 cut(s) 122, 268
Bst6I CTCTTC 2 cut(s) 744, 767
BstBI TTCGAA 1 cut(s) 192
BstC8I GCNNGC 2 cut(s) 149, 262
BstDEI CTNAG 2 cut(s) 129, 779
BstF5I GGATG 4 cut(s) 313, 450, 493, 592
BstKTI GATC 3 cut(s) 237, 299, 807
BstMAI GTCTC 3 cut(s) 111, 181, 339
BstMBI GATC 3 cut(s) 234, 296, 804
BstMCI CGRYCG 1 cut(s) 124
BstMWI GCNNNNNNNGC 2 cut(s) 238, 674
BstNI CCWGG 3 cut(s) 95, 249, 842
BstNSI RCATGY 1 cut(s) 232
BstSCI CCNGG 3 cut(s) 93, 247, 840
BstV1I GCAGC 1 cut(s) 24
BsuI GTATCC 1 cut(s) 555
BsuRI GGCC 1 cut(s) 596
BtsCI GGATG 4 cut(s) 313, 450, 493, 592
BtsI GCAGTG 2 cut(s) 642, 663
BtsIMutI CAGTG 4 cut(s) 264, 642, 663, 819
Cac8I GCNNGC 2 cut(s) 149, 262
Cfr13I GGNCC 2 cut(s) 594, 787
Csp6I GTAC 1 cut(s) 492
CviAII CATG 6 cut(s) 111, 229, 303, 708, 738, 802
CviQI GTAC 1 cut(s) 492
DdeI CTNAG 2 cut(s) 129, 779
DpnI GATC 3 cut(s) 236, 298, 806
DpnII GATC 3 cut(s) 234, 296, 804
Eam1104I CTCTTC 2 cut(s) 744, 767
EarI CTCTTC 2 cut(s) 744, 767
Ecl136II GAGCTC 1 cut(s) 754
Eco130I CCWWGG 1 cut(s) 379
Eco24I GRGCYC 1 cut(s) 756
Eco31I GGTCTC 1 cut(s) 339
Eco47I GGWCC 1 cut(s) 787
Eco53kI GAGCTC 1 cut(s) 754
EcoICRI GAGCTC 1 cut(s) 754
EcoRII CCWGG 3 cut(s) 93, 247, 840
EcoT14I CCWWGG 1 cut(s) 379
EcoT22I ATGCAT 1 cut(s) 739
EcoT38I GRGCYC 1 cut(s) 756
ErhI CCWWGG 1 cut(s) 379
Esp3I CGTCTC 1 cut(s) 111
FaeI CATG 6 cut(s) 114, 232, 306, 711, 741, 805
FaqI GGGAC 1 cut(s) 230
FatI CATG 6 cut(s) 110, 228, 302, 707, 737, 801
Fnu4HI GCNGC 1 cut(s) 38
FokI GGATG 4 cut(s) 320, 437, 500, 599
FriOI GRGCYC 1 cut(s) 756
Fsp4HI GCNGC 1 cut(s) 38
FspBI CTAG 1 cut(s) 570
GluI GCNGC 1 cut(s) 38
GsaI CCCAGC 1 cut(s) 787
GsuI CTGGAG 1 cut(s) 363
HaeIII GGCC 1 cut(s) 596
Hin1II CATG 6 cut(s) 114, 232, 306, 711, 741, 805
HindIII AAGCTT 1 cut(s) 239
HinfI GANTC 6 cut(s) 43, 194, 460, 521, 701, 758
HphI GGTGA 1 cut(s) 711
Hpy166II GTNNAC 1 cut(s) 608
Hpy188I TCNGA 1 cut(s) 748
Hpy188III TCNNGA 3 cut(s) 457, 511, 577
Hpy8I GTNNAC 1 cut(s) 608
HpyAV CCTTC 1 cut(s) 19
HpyCH4III ACNGT 2 cut(s) 122, 268
HpyCH4V TGCA 7 cut(s) 188, 232, 471, 711, 737, 819, 861
HpyF10VI GCNNNNNNNGC 2 cut(s) 238, 674
HpyF3I CTNAG 2 cut(s) 129, 779
Hsp92II CATG 6 cut(s) 114, 232, 306, 711, 741, 805
Kzo9I GATC 3 cut(s) 234, 296, 804
LguI GCTCTTC 1 cut(s) 744
LmnI GCTCC 1 cut(s) 640
Lsp1109I GCAGC 1 cut(s) 24
MaeI CTAG 1 cut(s) 570
MaeIII GTNAC 3 cut(s) 83, 268, 332
MalI GATC 3 cut(s) 236, 298, 806
MboI GATC 3 cut(s) 234, 296, 804
MboII GAAGA 6 cut(s) 350, 696, 708, 761, 781, 784
MfeI CAATTG 1 cut(s) 525
MhlI GDGCHC 2 cut(s) 637, 756
MluCI AATT 8 cut(s) 98, 404, 474, 498, 525, 658, 762, 811
MlyI GAGTC 1 cut(s) 37
MnlI CCTC 7 cut(s) 57, 85, 422, 464, 552, 584, 768
Mph1103I ATGCAT 1 cut(s) 739
MseI TTAA 4 cut(s) 32, 65, 90, 868
MslI CAYNNNNRTG 1 cut(s) 366
MspR9I CCNGG 3 cut(s) 95, 249, 842
MunI CAATTG 1 cut(s) 525
Mva1269I GAATGC 3 cut(s) 188, 586, 863
MvaI CCWGG 3 cut(s) 95, 249, 842
MwoI GCNNNNNNNGC 2 cut(s) 238, 674
NdeII GATC 3 cut(s) 234, 296, 804
NlaIII CATG 6 cut(s) 114, 232, 306, 711, 741, 805
NlaIV GGNNCC 1 cut(s) 788
NmuCI GTSAC 1 cut(s) 268
NsiI ATGCAT 1 cut(s) 739
NspI RCATGY 1 cut(s) 232
NspV TTCGAA 1 cut(s) 192
PasI CCCWGGG 1 cut(s) 841
PciSI GCTCTTC 1 cut(s) 744
PctI GAATGC 3 cut(s) 188, 586, 863
PfeI GAWTC 5 cut(s) 194, 460, 521, 701, 758
PflFI GACNNNGTC 1 cut(s) 173
PflMI CCANNNNNTGG 1 cut(s) 456
PkrI GCNGC 1 cut(s) 39
PleI GAGTC 1 cut(s) 37
PpsI GAGTC 1 cut(s) 37
Psp124BI GAGCTC 1 cut(s) 756
Psp6I CCWGG 3 cut(s) 93, 247, 840
PspFI CCCAGC 1 cut(s) 783
PspGI CCWGG 3 cut(s) 93, 247, 840
PspN4I GGNNCC 1 cut(s) 788
PspPI GGNCC 2 cut(s) 594, 787
PsyI GACNNNGTC 1 cut(s) 173
RsaI GTAC 1 cut(s) 493
RsaNI GTAC 1 cut(s) 492
RseI CAYNNNNRTG 1 cut(s) 366
SacI GAGCTC 1 cut(s) 756
SapI GCTCTTC 1 cut(s) 744
SaqAI TTAA 4 cut(s) 32, 65, 90, 868
SatI GCNGC 1 cut(s) 38
Sau3AI GATC 3 cut(s) 234, 296, 804
Sau96I GGNCC 2 cut(s) 594, 787
SchI GAGTC 1 cut(s) 37
ScrFI CCNGG 3 cut(s) 95, 249, 842
SduI GDGCHC 2 cut(s) 637, 756
SetI ASST 8 cut(s) 62, 130, 153, 243, 414, 446, 756, 785
SfuI TTCGAA 1 cut(s) 192
SinI GGWCC 1 cut(s) 787
SmiMI CAYNNNNRTG 1 cut(s) 366
Sse9I AATT 8 cut(s) 98, 404, 474, 498, 525, 658, 762, 811
SsiI CCGC 1 cut(s) 584
SspI AATATT 1 cut(s) 69
SspMI CTAG 1 cut(s) 570
SstI GAGCTC 1 cut(s) 756
StyD4I CCNGG 3 cut(s) 93, 247, 840
StyI CCWWGG 1 cut(s) 379
TaaI ACNGT 2 cut(s) 122, 268
TaqI TCGA 3 cut(s) 124, 192, 756
TasI AATT 8 cut(s) 98, 404, 474, 498, 525, 658, 762, 811
TfiI GAWTC 5 cut(s) 194, 460, 521, 701, 758
Tru1I TTAA 4 cut(s) 32, 65, 90, 868
Tru9I TTAA 4 cut(s) 32, 65, 90, 868
TscAI CASTG 4 cut(s) 271, 649, 670, 826
TseFI GTSAC 1 cut(s) 268
TseI GCWGC 1 cut(s) 37
Tsp45I GTSAC 1 cut(s) 268
TspDTI ATGAA 3 cut(s) 15, 639, 696
TspGWI ACGGA 1 cut(s) 840
TspRI CASTG 4 cut(s) 271, 649, 670, 826
Tth111I GACNNNGTC 1 cut(s) 173
Van91I CCANNNNNTGG 1 cut(s) 456
VpaK11BI GGWCC 1 cut(s) 787
XapI RAATTY 3 cut(s) 98, 404, 658
XceI RCATGY 1 cut(s) 232
XspI CTAG 1 cut(s) 570
Zsp2I ATGCAT 1 cut(s) 739
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.