Rmu_sc0002451.1_g000069

Catalyzes xyloglucan endohydrolysis (XEH) and or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002451.1
OG0000460
Physical Location & Seq
Forward (+)
272780 .. 273864
1085 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0002451.1_g000069.1.cds

Sequence Viewer

Length: 882 bp
atggcttctttcaaatctgctctttcactgatgctgcttgctgtacctcttcttgttatggttgcctctgcgggtaacttcaaccaggactttcaaattacatggggagatggccgagccaagatactcaacaatggccaacttcttactctcacgctcgacaaagcctctggctctggtttcgaatccaaaaatgaatatcttttcggcaaaatcgatatgcagctcaagcttgttgctgggaactctgctggaactgtcaccgcctactatttacgctcagaaggatcgttatgggatgagatagactttgagttcttgggaaacctgagtggtgacccatacattcttcacaccaatgtgttcacccaaggcaaaggaaacagagaacaacaattctatctctggttcgacccaactgctgatttccacacctattccattctctggaatccacagcgcattgttttctctgtggatggaactcccattagagagttcaagaacatggaatcaattggcgttccattcccaaagaaccagccaatgagattaacctctagtctgtggaatgctgatgattgggcaacaaggggagggcttgtgaagacagactggagcaaagctcctttcactgcttcctacaggaacttcaatgccgacaaggcttgtatttggtcaaggactaacaaaacatcttcgtgcagctcgtcatcgtcatcgtcaagttctaaaatacctggtaatgaatggctcacagaagatctcgacacaacgaagcaggggaggctgagatgggtgcagaagaactacatgatctataattactgctctgacattaagagatttccccagggactccctactgaatgcaagtcataa

Protein Analysis

293

Amino Acids

32.99

Weight (kDa)

8.24

Isoelectric Point (pI)

36.08

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000460)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G25810 AT4G30270 AT5G57550 AT5G57560
fragaria_vesca FvH4_3g00833 FvH4_3g00840 FvH4_3g00850 FvH4_4g06700 FvH4_4g09230
malus_domestica MD13G1237300.v1.1 MD13G1268000.v1.1 MD13G1268900.v1.1 MD16G1267200.v1.1 MD16G1267300.v1.1
prunus_persica Prupe.1G069800_v2.0.a1 Prupe.1G088600_v2.0.a1 Prupe.1G088800_v2.0.a1 Prupe.1G088900_v2.0.a1 Prupe.1G089000_v2.0.a1
pyrus_communis pycom13g21020 pycom13g22350 pycom13g22380 pycom16g23630 pycom16g23640
rosa_chinensis RchiOBHm_Chr4g0398631 RchiOBHm_Chr4g0402891 RchiOBHm_Chr4g0402911 RchiOBHm_Chr4g0402921 RchiOBHm_Chr4g0402931 RchiOBHm_Chr4g0402951 RchiOBHm_Chr4g0402961 RchiOBHm_Chr4g0402981
rosa_laevigata RLG00000009031 RLG00000009033 RLG00000009034 RLG00000009035 RLG00000009039 RLG00000009040 RLG00000009339
rosa_multiflora Rmu_co8186292.1_g000001 Rmu_sc0000990.1_g000004 Rmu_sc0001023.1_g000002 Rmu_sc0001023.1_g000004 Rmu_sc0001023.1_g000007 Rmu_sc0001023.1_g000015 Rmu_sc0002178.1_g000007 Rmu_sc0002451.1_g000069 Rmu_sc0002451.1_g000079 Rmu_sc0002451.1_g000080 Rmu_sc0008058.1_g000026 Rmu_sc0008058.1_g000029 Rmu_sc0008058.1_g000030
rosa_roxburghii Rroxscaffold_5G00343590 Rroxscaffold_5G00347620 Rroxscaffold_5G00347630 Rroxscaffold_5G00347640 Rroxscaffold_5G00347650 Rroxscaffold_5G00347660 Rroxscaffold_5G00347670 Rroxscaffold_5G00347690
rosa_rugosa Rorug04G0009900 Rorug04G0039000 Rorug04G0039100 Rorug04G0039200 Rorug04G0039300 Rorug04G0039400 Rorug04G0039500 Rorug04G0039600 Rorug04G0039600 Rorug04G0040200
rosa_samantha Rh4AG087800 Rh4AG116500 Rh4AG116700 Rh4AG116800 Rh4AG116900 Rh4AG117000 Rh4AG117100 Rh4BG109300 Rh4BG109400 Rh4BG109500 Rh4BG109600 Rh4BG109700 Rh4BG109800 Rh4CG096100 Rh4CG123700 Rh4CG124200
rosa_wichuraiana Rw4G007280 Rw4G009320 Rw4G009340 Rw4G009370 Rw4G009380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 447
AciI CCGC 2 cut(s) 71, 264
AclWI GGATC 1 cut(s) 295
AcoI YGGCCR 2 cut(s) 112, 136
AfaI GTAC 1 cut(s) 45
AfiI CCNNNNNNNGG 1 cut(s) 447
AgsI TTSAA 5 cut(s) 13, 82, 95, 502, 655
AjnI CCWGG 3 cut(s) 84, 739, 852
AleI CACNNNNGTG 1 cut(s) 359
AluBI AGCT 4 cut(s) 226, 232, 626, 708
AluI AGCT 4 cut(s) 226, 232, 626, 708
AlwI GGATC 1 cut(s) 295
AoxI GGCC 2 cut(s) 112, 136
ApeKI GCWGC 3 cut(s) 34, 223, 705
AspLEI GCGC 1 cut(s) 462
AsuHPI GGTGA 3 cut(s) 253, 347, 358
AsuII TTCGAA 1 cut(s) 183
BalI TGGCCA 1 cut(s) 138
BbsI GAAGAC 1 cut(s) 614
BbvI GCAGC 3 cut(s) 21, 235, 717
BccI CCATC 3 cut(s) 104, 473, 789
BcgI CGANNNNNNTGC 2 cut(s) 401, 435
BciT130I CCWGG 3 cut(s) 86, 741, 854
BfaI CTAG 1 cut(s) 561
BfmI CTRYAG 1 cut(s) 643
BglI GCCNNNNNGGC 1 cut(s) 665
BglII AGATCT 1 cut(s) 763
BisI GCNGC 3 cut(s) 35, 224, 706
BlsI GCNGC 3 cut(s) 36, 225, 707
Bme1390I CCNGG 3 cut(s) 86, 741, 854
BmrFI CCNGG 3 cut(s) 86, 741, 854
BmsI GCATC 1 cut(s) 21
BpiI GAAGAC 1 cut(s) 614
BplI GAGNNNNNCTC 2 cut(s) 610, 642
BpmI CTGGAG 1 cut(s) 637
Bpu14I TTCGAA 1 cut(s) 183
BpuEI CTTGAG 1 cut(s) 212
Bsa29I ATCGAT 1 cut(s) 216
BsaJI CCNNGG 3 cut(s) 370, 852, 853
BsaXI ACNNNNNCTCC 2 cut(s) 588, 618
Bsc4I CCNNNNNNNGG 1 cut(s) 447
Bse1I ACTGG 1 cut(s) 620
BseBI CCWGG 3 cut(s) 86, 741, 854
BseCI ATCGAT 1 cut(s) 216
BseDI CCNNGG 3 cut(s) 370, 852, 853
BseGI GGATG 2 cut(s) 304, 484
BseLI CCNNNNNNNGG 1 cut(s) 447
BseMII CTCAG 3 cut(s) 294, 320, 782
BseNI ACTGG 1 cut(s) 620
BseXI GCAGC 3 cut(s) 21, 235, 717
BseYI CCCAGC 1 cut(s) 239
BsgI GTGCAG 2 cut(s) 724, 821
BshFI GGCC 2 cut(s) 114, 138
BshVI ATCGAT 1 cut(s) 216
BslFI GGGAC 1 cut(s) 870
BslI CCNNNNNNNGG 1 cut(s) 447
BsmFI GGGAC 1 cut(s) 870
BsmI GAATGC 2 cut(s) 577, 875
BsnI GGCC 2 cut(s) 114, 138
Bsp119I TTCGAA 1 cut(s) 183
Bsp143I GATC 3 cut(s) 287, 763, 816
BspACI CCGC 2 cut(s) 71, 264
BspANI GGCC 2 cut(s) 114, 138
BspCNI CTCAG 3 cut(s) 293, 321, 783
BspDI ATCGAT 1 cut(s) 216
BspPI GGATC 1 cut(s) 295
BspT104I TTCGAA 1 cut(s) 183
BsrI ACTGG 1 cut(s) 620
BssECI CCNNGG 3 cut(s) 370, 852, 853
BssMI GATC 3 cut(s) 287, 763, 816
BssT1I CCWWGG 1 cut(s) 370
Bst2UI CCWGG 3 cut(s) 86, 741, 854
Bst4CI ACNGT 1 cut(s) 259
Bst6I CTCTTC 1 cut(s) 54
BstBI TTCGAA 1 cut(s) 183
BstC8I GCNNGC 1 cut(s) 39
BstDEI CTNAG 3 cut(s) 280, 329, 791
BstEII GGTNACC 1 cut(s) 335
BstF5I GGATG 2 cut(s) 304, 484
BstHHI GCGC 1 cut(s) 462
BstKTI GATC 3 cut(s) 290, 766, 819
BstMBI GATC 3 cut(s) 287, 763, 816
BstMWI GCNNNNNNNGC 3 cut(s) 229, 665, 787
BstNI CCWGG 3 cut(s) 86, 741, 854
BstPI GGTNACC 1 cut(s) 335
BstSCI CCNGG 3 cut(s) 84, 739, 852
BstSFI CTRYAG 1 cut(s) 643
BstV1I GCAGC 3 cut(s) 21, 235, 717
BstV2I GAAGAC 1 cut(s) 614
BstX2I RGATCY 1 cut(s) 763
BstYI RGATCY 1 cut(s) 763
Bsu15I ATCGAT 1 cut(s) 216
BsuRI GGCC 2 cut(s) 114, 138
BsuTUI ATCGAT 1 cut(s) 216
BtsCI GGATG 2 cut(s) 304, 484
BtsI GCAGTG 1 cut(s) 633
BtsIMutI CAGTG 2 cut(s) 26, 633
Cac8I GCNNGC 1 cut(s) 39
CfoI GCGC 1 cut(s) 462
ClaI ATCGAT 1 cut(s) 216
CsiI ACCWGGT 1 cut(s) 739
Csp6I GTAC 1 cut(s) 44
CviAII CATG 3 cut(s) 102, 508, 814
CviQI GTAC 1 cut(s) 44
DdeI CTNAG 3 cut(s) 280, 329, 791
DpnI GATC 3 cut(s) 289, 765, 818
DpnII GATC 3 cut(s) 287, 763, 816
EaeI YGGCCR 2 cut(s) 112, 136
Eam1104I CTCTTC 1 cut(s) 54
EarI CTCTTC 1 cut(s) 54
Eco130I CCWWGG 1 cut(s) 370
Eco91I GGTNACC 1 cut(s) 335
EcoO65I GGTNACC 1 cut(s) 335
EcoRII CCWGG 3 cut(s) 84, 739, 852
EcoT14I CCWWGG 1 cut(s) 370
ErhI CCWWGG 1 cut(s) 370
FaeI CATG 3 cut(s) 105, 511, 817
FaiI YATR 9 cut(s) 59, 103, 221, 295, 343, 509, 815, 822, 880
FaqI GGGAC 1 cut(s) 870
FatI CATG 3 cut(s) 101, 507, 813
FauI CCCGC 1 cut(s) 64
Fnu4HI GCNGC 3 cut(s) 35, 224, 706
FokI GGATG 2 cut(s) 311, 491
Fsp4HI GCNGC 3 cut(s) 35, 224, 706
FspBI CTAG 1 cut(s) 561
GlaI GCGC 1 cut(s) 461
GluI GCNGC 3 cut(s) 35, 224, 706
GsaI CCCAGC 1 cut(s) 243
GsuI CTGGAG 1 cut(s) 637
HaeIII GGCC 2 cut(s) 114, 138
HhaI GCGC 1 cut(s) 462
Hin1II CATG 3 cut(s) 105, 511, 817
Hin6I GCGC 1 cut(s) 460
HinP1I GCGC 1 cut(s) 460
HindIII AAGCTT 1 cut(s) 230
HinfI GANTC 4 cut(s) 185, 451, 512, 858
HphI GGTGA 3 cut(s) 253, 347, 358
Hpy166II GTNNAC 1 cut(s) 366
Hpy188I TCNGA 2 cut(s) 283, 835
Hpy188III TCNNGA 3 cut(s) 448, 502, 767
Hpy8I GTNNAC 1 cut(s) 366
HpyAV CCTTC 1 cut(s) 278
HpyCH4III ACNGT 1 cut(s) 259
HpyCH4V TGCA 4 cut(s) 223, 705, 802, 873
HpyF10VI GCNNNNNNNGC 3 cut(s) 229, 665, 787
HpyF3I CTNAG 3 cut(s) 280, 329, 791
Hsp92II CATG 3 cut(s) 105, 511, 817
HspAI GCGC 1 cut(s) 460
Kzo9I GATC 3 cut(s) 287, 763, 816
LmnI GCTCC 2 cut(s) 618, 631
Lsp1109I GCAGC 3 cut(s) 21, 235, 717
LweI GCATC 1 cut(s) 21
MabI ACCWGGT 1 cut(s) 739
MaeI CTAG 1 cut(s) 561
MaeIII GTNAC 3 cut(s) 74, 259, 335
MalI GATC 3 cut(s) 289, 765, 818
MboI GATC 3 cut(s) 287, 763, 816
MboII GAAGA 6 cut(s) 41, 341, 619, 690, 773, 817
MfeI CAATTG 1 cut(s) 516
MflI RGATCY 1 cut(s) 763
MlsI TGGCCA 1 cut(s) 138
MluCI AATT 4 cut(s) 96, 395, 516, 823
MluNI TGGCCA 1 cut(s) 138
MlyI GAGTC 1 cut(s) 852
MnlI CCTC 6 cut(s) 57, 76, 178, 568, 590, 780
Mox20I TGGCCA 1 cut(s) 138
MscI TGGCCA 1 cut(s) 138
MseI TTAA 2 cut(s) 554, 840
MslI CAYNNNNRTG 3 cut(s) 357, 359, 700
Msp20I TGGCCA 1 cut(s) 138
MspR9I CCNGG 3 cut(s) 86, 741, 854
MunI CAATTG 1 cut(s) 516
Mva1269I GAATGC 2 cut(s) 577, 875
MvaI CCWGG 3 cut(s) 86, 741, 854
MwoI GCNNNNNNNGC 3 cut(s) 229, 665, 787
NdeII GATC 3 cut(s) 287, 763, 816
NlaIII CATG 3 cut(s) 105, 511, 817
NmeAIII GCCGAG 1 cut(s) 140
NmuCI GTSAC 2 cut(s) 259, 335
NspV TTCGAA 1 cut(s) 183
OliI CACNNNNGTG 1 cut(s) 359
PasI CCCWGGG 1 cut(s) 853
PcsI WCGNNNNNNNCGW 2 cut(s) 213, 707
PctI GAATGC 2 cut(s) 577, 875
PfeI GAWTC 3 cut(s) 185, 451, 512
PflMI CCANNNNNTGG 1 cut(s) 447
PkrI GCNGC 3 cut(s) 36, 225, 707
PleI GAGTC 1 cut(s) 852
PpsI GAGTC 1 cut(s) 852
Psp6I CCWGG 3 cut(s) 84, 739, 852
PspEI GGTNACC 1 cut(s) 335
PspFI CCCAGC 1 cut(s) 239
PspGI CCWGG 3 cut(s) 84, 739, 852
PsuI RGATCY 1 cut(s) 763
RsaI GTAC 1 cut(s) 45
RsaNI GTAC 1 cut(s) 44
RseI CAYNNNNRTG 3 cut(s) 357, 359, 700
SaqAI TTAA 2 cut(s) 554, 840
SatI GCNGC 3 cut(s) 35, 224, 706
Sau3AI GATC 3 cut(s) 287, 763, 816
SchI GAGTC 1 cut(s) 852
ScrFI CCNGG 3 cut(s) 86, 741, 854
SetI ASST 9 cut(s) 49, 228, 234, 330, 437, 560, 628, 710, 742
SexAI ACCWGGT 1 cut(s) 739
SfaNI GCATC 1 cut(s) 21
SfcI CTRYAG 1 cut(s) 643
SfuI TTCGAA 1 cut(s) 183
SmiMI CAYNNNNRTG 3 cut(s) 357, 359, 700
SmlI CTYRAG 1 cut(s) 227
SmoI CTYRAG 1 cut(s) 227
Sse9I AATT 4 cut(s) 96, 395, 516, 823
SsiI CCGC 2 cut(s) 71, 264
SspMI CTAG 1 cut(s) 561
StyD4I CCNGG 3 cut(s) 84, 739, 852
StyI CCWWGG 1 cut(s) 370
TaaI ACNGT 1 cut(s) 259
TaqI TCGA 5 cut(s) 159, 183, 216, 411, 768
TasI AATT 4 cut(s) 96, 395, 516, 823
TfiI GAWTC 3 cut(s) 185, 451, 512
Tru1I TTAA 2 cut(s) 554, 840
Tru9I TTAA 2 cut(s) 554, 840
TscAI CASTG 2 cut(s) 33, 640
TseFI GTSAC 2 cut(s) 259, 335
TseI GCWGC 3 cut(s) 34, 223, 705
Tsp45I GTSAC 2 cut(s) 259, 335
TspDTI ATGAA 2 cut(s) 210, 762
TspRI CASTG 2 cut(s) 33, 640
Van91I CCANNNNNTGG 1 cut(s) 447
XspI CTAG 1 cut(s) 561
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.