RLG00000009031

Catalyzes xyloglucan endohydrolysis (XEH) and or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0000460
Physical Location & Seq
Reverse (-)
45330834 .. 45331924
1091 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000009031

Sequence Viewer

Length: 867 bp
ATGGCTTCTTCAACTTCAGTGCTTCTGTTCTTTCCATTACTGCTAATTAGCTCTGTTATGGTTGCCTCTGCTGGTAACTTCAACCAAGACTTTAAAATTACATGGGGGGACGGTCGAGCTAACATGCTCAACGACGGCCAACTTCTAACTCTCTCCCTCGACAAAGCCTCTGGCTCTGGTTTTGAATCCAAAAACGAATACCTCTTCGGCAAGATTGACATGCAGCTCAAGCTTGTCGCCGGAAACTCTGCCGGCACCGTCACAGCCTACTACTTAAAATCAGAAGGGTCTACGTGGGATGAGATAGACTTTGAGTTCTTGGGAAACCTGAGTGGCAACCCTTACACTCTTCACACTAATGTCTTCAGCCAAGGCAAAGGAAACAGAGAACAGCAATTCCATCTCTGGTTCGACCCAACTGCTGATTTCCACACCTATTCCATTCTCTGGAATCCACAGCGCATTGTTTTCTCTGTTGATGGTACTCCCATTAGAGAGTTCAAGAACCAAGAGTCGAATGGTGTTCCATTCCCAAAGAGCCAAGCAATGAGAATACACTCGAGCCTCTGGAATGCTGATGATTGGGCAACAATGGGAGGACGTGTAAAGACGGATTGGAATGCAGCTCCGTTCACTGCCTCTTACAGGAATTTCAATGCTGAAGCATGCATTTGGGCTTCAGGATCATCTTCTTGCGGTTCAAGCAGTGCCCCTGCTTCGACTAATGGAGATTGGCTTTCGCAAGAATTGGACAGTGCTAGTTATGAAAAGCTGAGTTGGGTGCAGAAAAACTACATGATCTACAATTACTGCACAGATGTCAACAGATTCCCTCAGGGCCTCCCAGTTGAATGCAGCACTTCATAG

Protein Analysis

289

Amino Acids

31.96

Weight (kDa)

4.99

Isoelectric Point (pI)

28.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_16 PF00722 27 - 207 1e-67 Glycosyl hydrolases family 16
XET_C PF06955 241 - 285 1.3e-19 Xyloglucan endo-transglycosylase (XET) C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000460)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G25810 AT4G30270 AT5G57550 AT5G57560
fragaria_vesca FvH4_3g00833 FvH4_3g00840 FvH4_3g00850 FvH4_4g06700 FvH4_4g09230
malus_domestica MD13G1237300.v1.1 MD13G1268000.v1.1 MD13G1268900.v1.1 MD16G1267200.v1.1 MD16G1267300.v1.1
prunus_persica Prupe.1G069800_v2.0.a1 Prupe.1G088600_v2.0.a1 Prupe.1G088800_v2.0.a1 Prupe.1G088900_v2.0.a1 Prupe.1G089000_v2.0.a1
pyrus_communis pycom13g21020 pycom13g22350 pycom13g22380 pycom16g23630 pycom16g23640
rosa_chinensis RchiOBHm_Chr4g0398631 RchiOBHm_Chr4g0402891 RchiOBHm_Chr4g0402911 RchiOBHm_Chr4g0402921 RchiOBHm_Chr4g0402931 RchiOBHm_Chr4g0402951 RchiOBHm_Chr4g0402961 RchiOBHm_Chr4g0402981
rosa_laevigata RLG00000009031 RLG00000009033 RLG00000009034 RLG00000009035 RLG00000009039 RLG00000009040 RLG00000009339
rosa_multiflora Rmu_co8186292.1_g000001 Rmu_sc0000990.1_g000004 Rmu_sc0001023.1_g000002 Rmu_sc0001023.1_g000004 Rmu_sc0001023.1_g000007 Rmu_sc0001023.1_g000015 Rmu_sc0002178.1_g000007 Rmu_sc0002451.1_g000069 Rmu_sc0002451.1_g000079 Rmu_sc0002451.1_g000080 Rmu_sc0008058.1_g000026 Rmu_sc0008058.1_g000029 Rmu_sc0008058.1_g000030
rosa_roxburghii Rroxscaffold_5G00343590 Rroxscaffold_5G00347620 Rroxscaffold_5G00347630 Rroxscaffold_5G00347640 Rroxscaffold_5G00347650 Rroxscaffold_5G00347660 Rroxscaffold_5G00347670 Rroxscaffold_5G00347690
rosa_rugosa Rorug04G0009900 Rorug04G0039000 Rorug04G0039100 Rorug04G0039200 Rorug04G0039300 Rorug04G0039400 Rorug04G0039500 Rorug04G0039600 Rorug04G0039600 Rorug04G0040200
rosa_samantha Rh4AG087800 Rh4AG116500 Rh4AG116700 Rh4AG116800 Rh4AG116900 Rh4AG117000 Rh4AG117100 Rh4BG109300 Rh4BG109400 Rh4BG109500 Rh4BG109600 Rh4BG109700 Rh4BG109800 Rh4CG096100 Rh4CG123700 Rh4CG124200
rosa_wichuraiana Rw4G007280 Rw4G009320 Rw4G009340 Rw4G009370 Rw4G009380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 254
AccB7I CCANNNNNTGG 1 cut(s) 447
AccI GTMKAC 1 cut(s) 290
AciI CCGC 1 cut(s) 696
AclWI GGATC 1 cut(s) 691
AcoI YGGCCR 1 cut(s) 136
AcsI RAATTY 1 cut(s) 649
AcuI CTGAAG 3 cut(s) 349, 663, 681
AfaI GTAC 1 cut(s) 484
AfiI CCNNNNNNNGG 2 cut(s) 447, 645
AflIII ACRYGT 1 cut(s) 601
AgsI TTSAA 7 cut(s) 12, 82, 185, 502, 655, 702, 851
AjiI CACGTC 1 cut(s) 602
AloI GAACNNNNNNTCC 2 cut(s) 381, 413
AluBI AGCT 6 cut(s) 51, 119, 226, 232, 626, 772
AluI AGCT 6 cut(s) 51, 119, 226, 232, 626, 772
AlwI GGATC 1 cut(s) 691
Ama87I CYCGRG 1 cut(s) 559
AoxI GGCC 2 cut(s) 136, 838
ApeKI GCWGC 3 cut(s) 223, 623, 855
ApoI RAATTY 1 cut(s) 649
AspLEI GCGC 1 cut(s) 462
AspS9I GGNCC 1 cut(s) 838
AvaI CYCGRG 1 cut(s) 559
AxyI CCTNAGG 1 cut(s) 834
BaeGI GKGCMC 1 cut(s) 712
BanI GGYRCC 1 cut(s) 254
BbsI GAAGAC 1 cut(s) 355
BbvI GCAGC 2 cut(s) 235, 635
BccI CCATC 2 cut(s) 408, 473
BceAI ACGGC 1 cut(s) 151
BcgI CGANNNNNNTGC 2 cut(s) 401, 435
BfaI CTAG 1 cut(s) 759
BisI GCNGC 3 cut(s) 224, 624, 856
BlsI GCNGC 3 cut(s) 225, 625, 857
BmeT110I CYCGRG 1 cut(s) 559
BmgBI CACGTC 1 cut(s) 602
BmgT120I GGNCC 1 cut(s) 838
BmiI GGNNCC 1 cut(s) 256
BmrI ACTGGG 1 cut(s) 839
BmuI ACTGGG 1 cut(s) 839
BpiI GAAGAC 1 cut(s) 355
BpuEI CTTGAG 1 cut(s) 212
BsaAI YACGTR 1 cut(s) 294
BsaJI CCNNGG 1 cut(s) 370
BsaXI ACNNNNNCTCC 2 cut(s) 588, 618
Bsc4I CCNNNNNNNGG 2 cut(s) 447, 645
Bse118I RCCGGY 1 cut(s) 251
Bse1I ACTGG 1 cut(s) 845
Bse21I CCTNAGG 1 cut(s) 834
Bse3DI GCAATG 1 cut(s) 552
BseDI CCNNGG 1 cut(s) 370
BseGI GGATG 1 cut(s) 304
BseLI CCNNNNNNNGG 2 cut(s) 447, 645
BseMI GCAATG 1 cut(s) 552
BseMII CTCAG 3 cut(s) 320, 764, 848
BseNI ACTGG 1 cut(s) 845
BseSI GKGCMC 1 cut(s) 712
BseXI GCAGC 2 cut(s) 235, 635
BsgI GTGCAG 2 cut(s) 796, 803
Bsh1285I CGRYCG 1 cut(s) 115
BshFI GGCC 2 cut(s) 138, 840
BshNI GGYRCC 1 cut(s) 254
BsiEI CGRYCG 1 cut(s) 115
BsiHKCI CYCGRG 1 cut(s) 559
BsiSI CCGG 2 cut(s) 240, 252
BslFI GGGAC 1 cut(s) 122
BslI CCNNNNNNNGG 2 cut(s) 447, 645
BsmFI GGGAC 1 cut(s) 122
BsmI GAATGC 3 cut(s) 577, 625, 857
BsnI GGCC 2 cut(s) 138, 840
BsoBI CYCGRG 1 cut(s) 559
Bsp1286I GDGCHC 1 cut(s) 712
Bsp143I GATC 2 cut(s) 683, 798
BspACI CCGC 1 cut(s) 696
BspANI GGCC 2 cut(s) 138, 840
BspCNI CTCAG 3 cut(s) 321, 765, 847
BspLI GGNNCC 1 cut(s) 256
BspPI GGATC 1 cut(s) 691
BspT107I GGYRCC 1 cut(s) 254
BsrDI GCAATG 1 cut(s) 552
BsrFI RCCGGY 1 cut(s) 251
BsrI ACTGG 1 cut(s) 845
BssAI RCCGGY 1 cut(s) 251
BssECI CCNNGG 1 cut(s) 370
BssMI GATC 2 cut(s) 683, 798
BssT1I CCWWGG 1 cut(s) 370
Bst4CI ACNGT 3 cut(s) 113, 259, 755
Bst6I CTCTTC 2 cut(s) 209, 354
BstBAI YACGTR 1 cut(s) 294
BstC8I GCNNGC 2 cut(s) 253, 667
BstDEI CTNAG 3 cut(s) 329, 773, 834
BstENI CCTNNNNNAGG 1 cut(s) 643
BstF5I GGATG 1 cut(s) 304
BstHHI GCGC 1 cut(s) 462
BstKTI GATC 2 cut(s) 686, 801
BstMBI GATC 2 cut(s) 683, 798
BstMCI CGRYCG 1 cut(s) 115
BstMWI GCNNNNNNNGC 2 cut(s) 229, 702
BstNSI RCATGY 3 cut(s) 127, 223, 669
BstSLI GKGCMC 1 cut(s) 712
BstV1I GCAGC 2 cut(s) 235, 635
BstV2I GAAGAC 1 cut(s) 355
Bsu36I CCTNAGG 1 cut(s) 834
BsuRI GGCC 2 cut(s) 138, 840
BtrI CACGTC 1 cut(s) 602
BtsCI GGATG 1 cut(s) 304
BtsI GCAGTG 2 cut(s) 633, 712
BtsIMutI CAGTG 4 cut(s) 24, 633, 712, 760
Cac8I GCNNGC 2 cut(s) 253, 667
CfoI GCGC 1 cut(s) 462
Cfr10I RCCGGY 1 cut(s) 251
Cfr13I GGNCC 1 cut(s) 838
Csp6I GTAC 1 cut(s) 483
CviAII CATG 5 cut(s) 102, 124, 220, 666, 796
CviQI GTAC 1 cut(s) 483
DdeI CTNAG 3 cut(s) 329, 773, 834
DpnI GATC 2 cut(s) 685, 800
DpnII GATC 2 cut(s) 683, 798
DraI TTTAAA 1 cut(s) 94
EaeI YGGCCR 1 cut(s) 136
Eam1104I CTCTTC 2 cut(s) 209, 354
EarI CTCTTC 2 cut(s) 209, 354
Eco130I CCWWGG 1 cut(s) 370
Eco57I CTGAAG 3 cut(s) 349, 663, 681
Eco81I CCTNAGG 1 cut(s) 834
Eco88I CYCGRG 1 cut(s) 559
EcoNI CCTNNNNNAGG 1 cut(s) 643
EcoO109I RGGNCCY 1 cut(s) 838
EcoT14I CCWWGG 1 cut(s) 370
EcoT22I ATGCAT 1 cut(s) 671
ErhI CCWWGG 1 cut(s) 370
FaeI CATG 5 cut(s) 105, 127, 223, 669, 799
FaiI YATR 8 cut(s) 59, 103, 125, 221, 667, 765, 797, 865
FaqI GGGAC 1 cut(s) 122
FatI CATG 5 cut(s) 101, 123, 219, 665, 795
FblI GTMKAC 1 cut(s) 290
Fnu4HI GCNGC 3 cut(s) 224, 624, 856
FokI GGATG 1 cut(s) 311
Fsp4HI GCNGC 3 cut(s) 224, 624, 856
FspBI CTAG 1 cut(s) 759
GlaI GCGC 1 cut(s) 461
GluI GCNGC 3 cut(s) 224, 624, 856
HaeIII GGCC 2 cut(s) 138, 840
HapII CCGG 2 cut(s) 240, 252
HhaI GCGC 1 cut(s) 462
Hin1II CATG 5 cut(s) 105, 127, 223, 669, 799
Hin6I GCGC 1 cut(s) 460
HinP1I GCGC 1 cut(s) 460
HincII GTYRAC 1 cut(s) 823
HindII GTYRAC 1 cut(s) 823
HindIII AAGCTT 1 cut(s) 230
HinfI GANTC 4 cut(s) 185, 451, 512, 828
HpaII CCGG 2 cut(s) 240, 252
Hpy166II GTNNAC 3 cut(s) 291, 633, 823
Hpy188I TCNGA 1 cut(s) 283
Hpy188III TCNNGA 4 cut(s) 448, 502, 568, 681
Hpy8I GTNNAC 3 cut(s) 291, 633, 823
Hpy99I CGWCG 1 cut(s) 137
HpyAV CCTTC 1 cut(s) 278
HpyCH4III ACNGT 3 cut(s) 113, 259, 755
HpyCH4IV ACGT 2 cut(s) 293, 601
HpyCH4V TGCA 6 cut(s) 223, 623, 669, 784, 813, 855
HpyF10VI GCNNNNNNNGC 2 cut(s) 229, 702
HpyF3I CTNAG 3 cut(s) 329, 773, 834
HpySE526I ACGT 2 cut(s) 293, 601
Hsp92II CATG 5 cut(s) 105, 127, 223, 669, 799
HspAI GCGC 1 cut(s) 460
KroI GCCGGC 1 cut(s) 251
KroNI GCCGGC 1 cut(s) 253
Kzo9I GATC 2 cut(s) 683, 798
LmnI GCTCC 1 cut(s) 631
Lsp1109I GCAGC 2 cut(s) 235, 635
MaeI CTAG 1 cut(s) 759
MaeII ACGT 2 cut(s) 293, 601
MaeIII GTNAC 2 cut(s) 74, 259
MalI GATC 2 cut(s) 685, 800
MboI GATC 2 cut(s) 683, 798
MboII GAAGA 4 cut(s) 196, 341, 355, 681
MhlI GDGCHC 1 cut(s) 712
MluCI AATT 6 cut(s) 45, 96, 395, 649, 746, 805
MlyI GAGTC 1 cut(s) 521
MnlI CCTC 9 cut(s) 76, 167, 178, 212, 575, 590, 649, 843, 851
Mph1103I ATGCAT 1 cut(s) 671
MroNI GCCGGC 1 cut(s) 251
MseI TTAA 2 cut(s) 93, 275
MslI CAYNNNNRTG 1 cut(s) 357
MspI CCGG 2 cut(s) 240, 252
Mva1269I GAATGC 3 cut(s) 577, 625, 857
MwoI GCNNNNNNNGC 2 cut(s) 229, 702
NaeI GCCGGC 1 cut(s) 253
NdeII GATC 2 cut(s) 683, 798
NgoMIV GCCGGC 1 cut(s) 251
NlaIII CATG 5 cut(s) 105, 127, 223, 669, 799
NlaIV GGNNCC 1 cut(s) 256
NmuCI GTSAC 1 cut(s) 259
NsiI ATGCAT 1 cut(s) 671
NspI RCATGY 3 cut(s) 127, 223, 669
PaeI GCATGC 1 cut(s) 669
PaeR7I CTCGAG 1 cut(s) 559
PctI GAATGC 3 cut(s) 577, 625, 857
PdiI GCCGGC 1 cut(s) 253
PfeI GAWTC 3 cut(s) 185, 451, 828
PflMI CCANNNNNTGG 1 cut(s) 447
PkrI GCNGC 3 cut(s) 225, 625, 857
PleI GAGTC 1 cut(s) 520
PpsI GAGTC 1 cut(s) 520
Ppu21I YACGTR 1 cut(s) 294
PspN4I GGNNCC 1 cut(s) 256
PspPI GGNCC 1 cut(s) 838
PspXI VCTCGAGB 1 cut(s) 559
RsaI GTAC 1 cut(s) 484
RsaNI GTAC 1 cut(s) 483
RseI CAYNNNNRTG 1 cut(s) 357
SaqAI TTAA 2 cut(s) 93, 275
SatI GCNGC 3 cut(s) 224, 624, 856
Sau3AI GATC 2 cut(s) 683, 798
Sau96I GGNCC 1 cut(s) 838
SchI GAGTC 1 cut(s) 521
SduI GDGCHC 1 cut(s) 712
Sfr274I CTCGAG 1 cut(s) 559
SlaI CTCGAG 1 cut(s) 559
SmiMI CAYNNNNRTG 1 cut(s) 357
SmlI CTYRAG 2 cut(s) 227, 559
SmoI CTYRAG 2 cut(s) 227, 559
SphI GCATGC 1 cut(s) 669
Sse9I AATT 6 cut(s) 45, 96, 395, 649, 746, 805
SsiI CCGC 1 cut(s) 696
SspMI CTAG 1 cut(s) 759
StyI CCWWGG 1 cut(s) 370
TaaI ACNGT 3 cut(s) 113, 259, 755
TaiI ACGT 2 cut(s) 296, 604
TaqI TCGA 6 cut(s) 115, 159, 411, 515, 560, 719
TasI AATT 6 cut(s) 45, 96, 395, 649, 746, 805
TfiI GAWTC 3 cut(s) 185, 451, 828
Tru1I TTAA 2 cut(s) 93, 275
Tru9I TTAA 2 cut(s) 93, 275
TscAI CASTG 4 cut(s) 24, 640, 712, 760
TseFI GTSAC 1 cut(s) 259
TseI GCWGC 3 cut(s) 223, 623, 855
Tsp45I GTSAC 1 cut(s) 259
TspDTI ATGAA 2 cut(s) 780, 852
TspGWI ACGGA 2 cut(s) 618, 626
TspRI CASTG 4 cut(s) 24, 640, 712, 760
Van91I CCANNNNNTGG 1 cut(s) 447
XagI CCTNNNNNAGG 1 cut(s) 643
XapI RAATTY 1 cut(s) 649
XceI RCATGY 3 cut(s) 127, 223, 669
XcmI CCANNNNNNNNNTGG 1 cut(s) 515
XhoI CTCGAG 1 cut(s) 559
XmiI GTMKAC 1 cut(s) 290
XspI CTAG 1 cut(s) 759
Zsp2I ATGCAT 1 cut(s) 671
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.