FvH4_3g00850

Catalyzes xyloglucan endohydrolysis (XEH) and or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0000460
Physical Location & Seq
Reverse (-)
431281 .. 432781
1501 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g00850.t1

Sequence Viewer

Length: 861 bp
ATGTCTTCTTCCACTACTTCAGTAGCGCTGCTGATCATTCCTCTACTTGTAGTTAGCTCTTTCATGGTTGCCTCTGCTGGTAACTTGAACCAAGACTTCAAGATTACATGGGGCGACGGTCGAGCCAAGATACTCAACGACGGTCAACTTCTTACTCTCTCCCTCGATAAAGCCTCTGGCTCTGGTTTTGAATCCACAAACGAATATCTCTTCGGCAAGATTGACATGCAGCTCAAGCTTGTTGCCGGAAACTCTGCTGGCACCGTCACTGCCTACTATTTAAAATCAGAGGGGTCTACATGGGATGAGATAGACTTTGAGTTCTTGGGTAACCTGAGTGGCGACCCTTACATTCTTCATACCAATATCTTCAGTCAAGGCAAAGGAAACAGAGAGCAGCAATTCTACCTCTGGTTCGACCCAACTGCTGATTTCCACACCTATTCCATTCTCTGGAATCCACAGCGCATTGTTTTTTCTGTTGATGGCACTCCCATTAGAGAGTTCAAGAATCAAGAGTCCAACGGTGTTCCATTCTTGAAGAGCCAAGCGATGAGAATACACTCGAGTCTCTGGAACGCCGACGATTGGGCAACAAGAGGTGGACTTGTGAAGACAGACTGGAGTGCAGCTCCTTTCACTGCCTCTTACAGGAATTTTAACGCTGAAGCTTGCATTTGGTCTTCTGGAGCATCTTCTTGCAGTTCAACTAGTGCTAATGGCGATTGGCTTTCACAAGAATTGGACAATACAAGTCAAGAAAGGATGAAATGGGTGCAGAAGAACTACATGATCTACAATTACTGTGCAGATGTCAAGCGTTTCCCTCAGGGCCTCCCTGTGGAATGCAGCACTTCCTAG

Protein Analysis

287

Amino Acids

31.84

Weight (kDa)

5.01

Isoelectric Point (pI)

31.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_16 PF00722 30 - 209 2.5e-65 Glycosyl hydrolases family 16
XET_C PF06955 240 - 283 5.3e-21 Xyloglucan endo-transglycosylase (XET) C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000460)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G25810 AT4G30270 AT5G57550 AT5G57560
fragaria_vesca FvH4_3g00833 FvH4_3g00840 FvH4_3g00850 FvH4_4g06700 FvH4_4g09230
malus_domestica MD13G1237300.v1.1 MD13G1268000.v1.1 MD13G1268900.v1.1 MD16G1267200.v1.1 MD16G1267300.v1.1
prunus_persica Prupe.1G069800_v2.0.a1 Prupe.1G088600_v2.0.a1 Prupe.1G088800_v2.0.a1 Prupe.1G088900_v2.0.a1 Prupe.1G089000_v2.0.a1
pyrus_communis pycom13g21020 pycom13g22350 pycom13g22380 pycom16g23630 pycom16g23640
rosa_chinensis RchiOBHm_Chr4g0398631 RchiOBHm_Chr4g0402891 RchiOBHm_Chr4g0402911 RchiOBHm_Chr4g0402921 RchiOBHm_Chr4g0402931 RchiOBHm_Chr4g0402951 RchiOBHm_Chr4g0402961 RchiOBHm_Chr4g0402981
rosa_laevigata RLG00000009031 RLG00000009033 RLG00000009034 RLG00000009035 RLG00000009039 RLG00000009040 RLG00000009339
rosa_multiflora Rmu_co8186292.1_g000001 Rmu_sc0000990.1_g000004 Rmu_sc0001023.1_g000002 Rmu_sc0001023.1_g000004 Rmu_sc0001023.1_g000007 Rmu_sc0001023.1_g000015 Rmu_sc0002178.1_g000007 Rmu_sc0002451.1_g000069 Rmu_sc0002451.1_g000079 Rmu_sc0002451.1_g000080 Rmu_sc0008058.1_g000026 Rmu_sc0008058.1_g000029 Rmu_sc0008058.1_g000030
rosa_roxburghii Rroxscaffold_5G00343590 Rroxscaffold_5G00347620 Rroxscaffold_5G00347630 Rroxscaffold_5G00347640 Rroxscaffold_5G00347650 Rroxscaffold_5G00347660 Rroxscaffold_5G00347670 Rroxscaffold_5G00347690
rosa_rugosa Rorug04G0009900 Rorug04G0039000 Rorug04G0039100 Rorug04G0039200 Rorug04G0039300 Rorug04G0039400 Rorug04G0039500 Rorug04G0039600 Rorug04G0039600 Rorug04G0040200
rosa_samantha Rh4AG087800 Rh4AG116500 Rh4AG116700 Rh4AG116800 Rh4AG116900 Rh4AG117000 Rh4AG117100 Rh4BG109300 Rh4BG109400 Rh4BG109500 Rh4BG109600 Rh4BG109700 Rh4BG109800 Rh4CG096100 Rh4CG123700 Rh4CG124200
rosa_wichuraiana Rw4G007280 Rw4G009320 Rw4G009340 Rw4G009370 Rw4G009380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 260
AccB7I CCANNNNNTGG 1 cut(s) 453
AccI GTMKAC 1 cut(s) 296
AcsI RAATTY 1 cut(s) 655
AcuI CTGAAG 2 cut(s) 355, 687
AfeI AGCGCT 1 cut(s) 27
AfiI CCNNNNNNNGG 4 cut(s) 453, 588, 651, 841
AgsI TTSAA 6 cut(s) 88, 100, 191, 508, 541, 708
AhlI ACTAGT 1 cut(s) 710
AluBI AGCT 5 cut(s) 57, 232, 238, 632, 671
AluI AGCT 5 cut(s) 57, 232, 238, 632, 671
Alw26I GTCTC 1 cut(s) 575
Ama87I CYCGRG 1 cut(s) 565
Aor51HI AGCGCT 1 cut(s) 27
AoxI GGCC 1 cut(s) 832
ApeKI GCWGC 5 cut(s) 28, 229, 397, 629, 849
ApoI RAATTY 1 cut(s) 655
AspLEI GCGC 2 cut(s) 28, 468
AspS9I GGNCC 1 cut(s) 832
AvaI CYCGRG 1 cut(s) 565
AxyI CCTNAGG 1 cut(s) 828
BanI GGYRCC 1 cut(s) 260
BbsI GAAGAC 2 cut(s) 620, 675
BbvI GCAGC 4 cut(s) 15, 241, 409, 641
BccI CCATC 1 cut(s) 479
BcgI CGANNNNNNTGC 2 cut(s) 407, 441
BclI TGATCA 1 cut(s) 33
BcoDI GTCTC 1 cut(s) 575
BcuI ACTAGT 1 cut(s) 710
BfaI CTAG 2 cut(s) 711, 859
BfoI RGCGCY 1 cut(s) 29
BisI GCNGC 5 cut(s) 29, 230, 398, 630, 850
BlsI GCNGC 5 cut(s) 30, 231, 399, 631, 851
BmeT110I CYCGRG 1 cut(s) 565
BmgT120I GGNCC 1 cut(s) 832
BmiI GGNNCC 1 cut(s) 262
BmsI GCATC 1 cut(s) 701
BpiI GAAGAC 2 cut(s) 620, 675
BplI GAGNNNNNCTC 2 cut(s) 616, 648
BpmI CTGGAG 2 cut(s) 643, 708
BpuEI CTTGAG 1 cut(s) 218
Bsc4I CCNNNNNNNGG 4 cut(s) 453, 588, 651, 841
Bse1I ACTGG 1 cut(s) 626
Bse21I CCTNAGG 1 cut(s) 828
BseGI GGATG 2 cut(s) 310, 771
BseLI CCNNNNNNNGG 4 cut(s) 453, 588, 651, 841
BseMII CTCAG 2 cut(s) 326, 842
BseNI ACTGG 1 cut(s) 626
BseXI GCAGC 4 cut(s) 15, 241, 409, 641
BsgI GTGCAG 3 cut(s) 648, 797, 828
Bsh1285I CGRYCG 1 cut(s) 121
BshFI GGCC 1 cut(s) 834
BshNI GGYRCC 1 cut(s) 260
BsiEI CGRYCG 1 cut(s) 121
BsiHKCI CYCGRG 1 cut(s) 565
BsiSI CCGG 1 cut(s) 246
BslI CCNNNNNNNGG 4 cut(s) 453, 588, 651, 841
BsmAI GTCTC 1 cut(s) 575
BsmI GAATGC 1 cut(s) 851
BsnI GGCC 1 cut(s) 834
BsoBI CYCGRG 1 cut(s) 565
Bsp143I GATC 2 cut(s) 33, 792
BspANI GGCC 1 cut(s) 834
BspCNI CTCAG 2 cut(s) 327, 841
BspLI GGNNCC 1 cut(s) 262
BspQI GCTCTTC 1 cut(s) 536
BspT107I GGYRCC 1 cut(s) 260
BsrI ACTGG 1 cut(s) 626
BssMI GATC 2 cut(s) 33, 792
Bst4CI ACNGT 5 cut(s) 119, 143, 265, 527, 806
Bst6I CTCTTC 2 cut(s) 215, 536
BstC8I GCNNGC 2 cut(s) 259, 673
BstDEI CTNAG 2 cut(s) 335, 828
BstEII GGTNACC 1 cut(s) 329
BstENI CCTNNNNNAGG 1 cut(s) 649
BstF5I GGATG 2 cut(s) 310, 771
BstH2I RGCGCY 1 cut(s) 29
BstHHI GCGC 2 cut(s) 28, 468
BstKTI GATC 2 cut(s) 36, 795
BstMAI GTCTC 1 cut(s) 575
BstMBI GATC 2 cut(s) 33, 792
BstMCI CGRYCG 1 cut(s) 121
BstMWI GCNNNNNNNGC 1 cut(s) 235
BstNSI RCATGY 1 cut(s) 229
BstPI GGTNACC 1 cut(s) 329
BstV1I GCAGC 4 cut(s) 15, 241, 409, 641
BstV2I GAAGAC 2 cut(s) 620, 675
Bsu36I CCTNAGG 1 cut(s) 828
BsuRI GGCC 1 cut(s) 834
BtgZI GCGATG 1 cut(s) 566
BtsCI GGATG 2 cut(s) 310, 771
BtsI GCAGTG 2 cut(s) 267, 639
BtsIMutI CAGTG 2 cut(s) 267, 639
Cac8I GCNNGC 2 cut(s) 259, 673
CfoI GCGC 2 cut(s) 28, 468
Cfr13I GGNCC 1 cut(s) 832
CviAII CATG 5 cut(s) 64, 108, 226, 300, 790
DdeI CTNAG 2 cut(s) 335, 828
DpnI GATC 2 cut(s) 35, 794
DpnII GATC 2 cut(s) 33, 792
DraI TTTAAA 1 cut(s) 282
Eam1104I CTCTTC 2 cut(s) 215, 536
EarI CTCTTC 2 cut(s) 215, 536
Eco47III AGCGCT 1 cut(s) 27
Eco57I CTGAAG 2 cut(s) 355, 687
Eco81I CCTNAGG 1 cut(s) 828
Eco88I CYCGRG 1 cut(s) 565
Eco91I GGTNACC 1 cut(s) 329
EcoNI CCTNNNNNAGG 1 cut(s) 649
EcoO109I RGGNCCY 1 cut(s) 832
EcoO65I GGTNACC 1 cut(s) 329
FaeI CATG 5 cut(s) 67, 111, 229, 303, 793
FaiI YATR 6 cut(s) 65, 109, 227, 301, 360, 791
FatI CATG 5 cut(s) 63, 107, 225, 299, 789
FbaI TGATCA 1 cut(s) 33
FblI GTMKAC 1 cut(s) 296
Fnu4HI GCNGC 5 cut(s) 29, 230, 398, 630, 850
FokI GGATG 2 cut(s) 317, 778
Fsp4HI GCNGC 5 cut(s) 29, 230, 398, 630, 850
FspBI CTAG 2 cut(s) 711, 859
GlaI GCGC 2 cut(s) 27, 467
GluI GCNGC 5 cut(s) 29, 230, 398, 630, 850
GsuI CTGGAG 2 cut(s) 643, 708
HaeII RGCGCY 1 cut(s) 29
HaeIII GGCC 1 cut(s) 834
HapII CCGG 1 cut(s) 246
HhaI GCGC 2 cut(s) 28, 468
Hin1II CATG 5 cut(s) 67, 111, 229, 303, 793
Hin6I GCGC 2 cut(s) 26, 466
HinP1I GCGC 2 cut(s) 26, 466
HincII GTYRAC 1 cut(s) 146
HindII GTYRAC 1 cut(s) 146
HindIII AAGCTT 2 cut(s) 236, 669
HinfI GANTC 5 cut(s) 191, 457, 511, 518, 568
HpaII CCGG 1 cut(s) 246
Hpy166II GTNNAC 3 cut(s) 146, 297, 605
Hpy188I TCNGA 1 cut(s) 289
Hpy188III TCNNGA 8 cut(s) 100, 454, 508, 515, 538, 574, 687, 758
Hpy8I GTNNAC 3 cut(s) 146, 297, 605
Hpy99I CGWCG 3 cut(s) 119, 143, 587
HpyCH4III ACNGT 5 cut(s) 119, 143, 265, 527, 806
HpyCH4V TGCA 7 cut(s) 229, 629, 675, 702, 778, 809, 849
HpyF10VI GCNNNNNNNGC 1 cut(s) 235
HpyF3I CTNAG 2 cut(s) 335, 828
Hsp92II CATG 5 cut(s) 67, 111, 229, 303, 793
HspAI GCGC 2 cut(s) 26, 466
Ksp22I TGATCA 1 cut(s) 33
Kzo9I GATC 2 cut(s) 33, 792
LguI GCTCTTC 1 cut(s) 536
LmnI GCTCC 2 cut(s) 637, 689
Lsp1109I GCAGC 4 cut(s) 15, 241, 409, 641
LweI GCATC 1 cut(s) 701
MaeI CTAG 2 cut(s) 711, 859
MaeIII GTNAC 3 cut(s) 80, 265, 329
MalI GATC 2 cut(s) 35, 794
MboI GATC 2 cut(s) 33, 792
MboII GAAGA 8 cut(s) 202, 347, 361, 553, 625, 675, 687, 793
MluCI AATT 4 cut(s) 401, 655, 740, 799
MlyI GAGTC 2 cut(s) 527, 577
MmeI TCCRAC 1 cut(s) 546
MseI TTAA 2 cut(s) 281, 660
MspI CCGG 1 cut(s) 246
Mva1269I GAATGC 1 cut(s) 851
MwoI GCNNNNNNNGC 1 cut(s) 235
NdeII GATC 2 cut(s) 33, 792
NlaIII CATG 5 cut(s) 67, 111, 229, 303, 793
NlaIV GGNNCC 1 cut(s) 262
NmuCI GTSAC 1 cut(s) 265
NspI RCATGY 1 cut(s) 229
PaeR7I CTCGAG 1 cut(s) 565
PciSI GCTCTTC 1 cut(s) 536
PctI GAATGC 1 cut(s) 851
PfeI GAWTC 3 cut(s) 191, 457, 511
PflMI CCANNNNNTGG 1 cut(s) 453
PkrI GCNGC 5 cut(s) 30, 231, 399, 631, 851
PleI GAGTC 2 cut(s) 526, 576
PpsI GAGTC 2 cut(s) 526, 576
PspEI GGTNACC 1 cut(s) 329
PspN4I GGNNCC 1 cut(s) 262
PspPI GGNCC 1 cut(s) 832
PspXI VCTCGAGB 1 cut(s) 565
SapI GCTCTTC 1 cut(s) 536
SaqAI TTAA 2 cut(s) 281, 660
SatI GCNGC 5 cut(s) 29, 230, 398, 630, 850
Sau3AI GATC 2 cut(s) 33, 792
Sau96I GGNCC 1 cut(s) 832
SchI GAGTC 2 cut(s) 527, 577
SetI ASST 9 cut(s) 59, 234, 240, 336, 411, 443, 604, 634, 673
SfaNI GCATC 1 cut(s) 701
Sfr274I CTCGAG 1 cut(s) 565
SlaI CTCGAG 1 cut(s) 565
SmlI CTYRAG 2 cut(s) 233, 565
SmoI CTYRAG 2 cut(s) 233, 565
SpeI ACTAGT 1 cut(s) 710
Sse9I AATT 4 cut(s) 401, 655, 740, 799
SspMI CTAG 2 cut(s) 711, 859
TaaI ACNGT 5 cut(s) 119, 143, 265, 527, 806
TaqI TCGA 4 cut(s) 121, 165, 417, 566
TasI AATT 4 cut(s) 401, 655, 740, 799
TfiI GAWTC 3 cut(s) 191, 457, 511
Tru1I TTAA 2 cut(s) 281, 660
Tru9I TTAA 2 cut(s) 281, 660
TscAI CASTG 2 cut(s) 274, 646
TseFI GTSAC 1 cut(s) 265
TseI GCWGC 5 cut(s) 28, 229, 397, 629, 849
Tsp45I GTSAC 1 cut(s) 265
TspDTI ATGAA 3 cut(s) 52, 347, 782
TspRI CASTG 2 cut(s) 274, 646
Van91I CCANNNNNTGG 1 cut(s) 453
XagI CCTNNNNNAGG 1 cut(s) 649
XapI RAATTY 1 cut(s) 655
XceI RCATGY 1 cut(s) 229
XhoI CTCGAG 1 cut(s) 565
XmiI GTMKAC 1 cut(s) 296
XspI CTAG 2 cut(s) 711, 859
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.