Rroxscaffold_5G00347650

Catalyzes xyloglucan endohydrolysis (XEH) and or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0000460
Physical Location & Seq
Reverse (-)
19214980 .. 19216291
1312 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00347650.1

Sequence Viewer

Length: 996 bp
ATGCATGCTCATCTATATATACCTACCATGCTCTTCTCTTCTGCTTCAACTCATCCATCCTTCACCTTCTTCTCTTCATCGATCAGTTCTTCAACGAATCCTCACTTTCATACATTTAGCTACTTCATGGCTTCTTCCACTTCAGTAGTGTTGCTGATTATTCCTCTTCTTGTTAGCTCTTTCATGGTTACCTCTGCGGGTAACTTCAACCAAGACTTTAAGATTACATGGGGGGACGGTCGAGCCAAGATACTCAACGACGGTCAACTTCTTACTCTCTCGCTCGACAAAGCCTCTGGCTCTGGTTTTGAATCCAAAAACGAATATCTATTCGGCAAGATTGACATGCAGCTCAAGCTTGTCGCCGGAAACTCTGCTGGAACTGTCACAGCCTATTATTTGAAATCAGAAGACTTACTTATATGGTCTACTTGGGATGAGATAGACTTTGAGTTCTTGGGCAACTTGAGTGGTGACCCTTACATTCTTCACACCAATATCTTCAGCCAAGGCAAAGGCAACCGAGAGCAGCAATTTCACCTCTGGTTCGACCCAACTGCTGATTTCCACACCTATTCCATTCTCTGGAATCCACAGCGCATTGTTTTTTCAGTTGATGGCACTCCCATTAGAGAGTTCAAGAATCAAGAGTCGAAAGGTGTTCCATTCTTGAAGAGCCAAGCAATGAGAATACACTCGAGCCTTTGGAACGCCGATGATTGGGCAACAAGAGGTGGACTTGTGAAGACAGATTGGAGTGCAGCTCCGTTCACTGCCTCTTACAGGAATTTCAATGCTGAAGCTTGCATTTGGTCTTCCGGAGCATCTTCTTGCAGTTCAACTAGTAATCAAAATGGCGACTGGCTATCACAAGAGCTGGACAGTACAAGTCAAGAAAGGATGAAATGGGTGCAGAACAACTACATGATCTACAATTACTGCGCAGATGTCAAGCGGTTTCCTCAGGGCCTCCCTGTAGAATGCAACACTTCTTAG

Protein Analysis

331

Amino Acids

37.24

Weight (kDa)

5.68

Isoelectric Point (pI)

31.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_16 PF00722 69 - 253 4.3e-65 Glycosyl hydrolases family 16
XET_C PF06955 285 - 328 9.7e-21 Xyloglucan endo-transglycosylase (XET) C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000460)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G25810 AT4G30270 AT5G57550 AT5G57560
fragaria_vesca FvH4_3g00833 FvH4_3g00840 FvH4_3g00850 FvH4_4g06700 FvH4_4g09230
malus_domestica MD13G1237300.v1.1 MD13G1268000.v1.1 MD13G1268900.v1.1 MD16G1267200.v1.1 MD16G1267300.v1.1
prunus_persica Prupe.1G069800_v2.0.a1 Prupe.1G088600_v2.0.a1 Prupe.1G088800_v2.0.a1 Prupe.1G088900_v2.0.a1 Prupe.1G089000_v2.0.a1
pyrus_communis pycom13g21020 pycom13g22350 pycom13g22380 pycom16g23630 pycom16g23640
rosa_chinensis RchiOBHm_Chr4g0398631 RchiOBHm_Chr4g0402891 RchiOBHm_Chr4g0402911 RchiOBHm_Chr4g0402921 RchiOBHm_Chr4g0402931 RchiOBHm_Chr4g0402951 RchiOBHm_Chr4g0402961 RchiOBHm_Chr4g0402981
rosa_laevigata RLG00000009031 RLG00000009033 RLG00000009034 RLG00000009035 RLG00000009039 RLG00000009040 RLG00000009339
rosa_multiflora Rmu_co8186292.1_g000001 Rmu_sc0000990.1_g000004 Rmu_sc0001023.1_g000002 Rmu_sc0001023.1_g000004 Rmu_sc0001023.1_g000007 Rmu_sc0001023.1_g000015 Rmu_sc0002178.1_g000007 Rmu_sc0002451.1_g000069 Rmu_sc0002451.1_g000079 Rmu_sc0002451.1_g000080 Rmu_sc0008058.1_g000026 Rmu_sc0008058.1_g000029 Rmu_sc0008058.1_g000030
rosa_roxburghii Rroxscaffold_5G00343590 Rroxscaffold_5G00347620 Rroxscaffold_5G00347630 Rroxscaffold_5G00347640 Rroxscaffold_5G00347650 Rroxscaffold_5G00347660 Rroxscaffold_5G00347670 Rroxscaffold_5G00347690
rosa_rugosa Rorug04G0009900 Rorug04G0039000 Rorug04G0039100 Rorug04G0039200 Rorug04G0039300 Rorug04G0039400 Rorug04G0039500 Rorug04G0039600 Rorug04G0039600 Rorug04G0040200
rosa_samantha Rh4AG087800 Rh4AG116500 Rh4AG116700 Rh4AG116800 Rh4AG116900 Rh4AG117000 Rh4AG117100 Rh4BG109300 Rh4BG109400 Rh4BG109500 Rh4BG109600 Rh4BG109700 Rh4BG109800 Rh4CG096100 Rh4CG123700 Rh4CG124200
rosa_wichuraiana Rw4G007280 Rw4G009320 Rw4G009340 Rw4G009370 Rw4G009380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 943
AccB7I CCANNNNNTGG 1 cut(s) 585
AccI GTMKAC 1 cut(s) 428
AccIII TCCGGA 1 cut(s) 818
AciI CCGC 2 cut(s) 197, 955
AcsI RAATTY 1 cut(s) 787
AcuI CTGAAG 3 cut(s) 126, 487, 819
AfaI GTAC 1 cut(s) 886
AfiI CCNNNNNNNGG 3 cut(s) 585, 720, 783
AgsI TTSAA 9 cut(s) 48, 93, 208, 311, 403, 640, 673, 793, 840
AhlI ACTAGT 1 cut(s) 842
AluBI AGCT 7 cut(s) 120, 177, 352, 358, 764, 803, 877
AluI AGCT 7 cut(s) 120, 177, 352, 358, 764, 803, 877
Ama87I CYCGRG 1 cut(s) 697
Aor13HI TCCGGA 1 cut(s) 818
AoxI GGCC 1 cut(s) 967
ApeKI GCWGC 3 cut(s) 349, 529, 761
ApoI RAATTY 1 cut(s) 787
AspLEI GCGC 2 cut(s) 600, 944
AspS9I GGNCC 1 cut(s) 967
AsuHPI GGTGA 3 cut(s) 55, 485, 530
AvaI CYCGRG 1 cut(s) 697
AxyI CCTNAGG 1 cut(s) 963
BbsI GAAGAC 3 cut(s) 417, 752, 807
BbvI GCAGC 3 cut(s) 361, 541, 773
BccI CCATC 2 cut(s) 64, 611
BcgI CGANNNNNNTGC 2 cut(s) 539, 573
BcuI ACTAGT 1 cut(s) 842
BfaI CTAG 1 cut(s) 843
BfmI CTRYAG 1 cut(s) 975
BisI GCNGC 3 cut(s) 350, 530, 762
BlsI GCNGC 3 cut(s) 351, 531, 763
BmeT110I CYCGRG 1 cut(s) 697
BmgT120I GGNCC 1 cut(s) 967
BmsI GCATC 1 cut(s) 833
BpiI GAAGAC 3 cut(s) 417, 752, 807
BplI GAGNNNNNCTC 2 cut(s) 748, 780
BpuEI CTTGAG 2 cut(s) 338, 487
Bsa29I ATCGAT 1 cut(s) 80
BsaJI CCNNGG 1 cut(s) 508
BsaWI WCCGGW 1 cut(s) 818
Bsc4I CCNNNNNNNGG 3 cut(s) 585, 720, 783
Bse1I ACTGG 1 cut(s) 866
Bse21I CCTNAGG 1 cut(s) 963
Bse3DI GCAATG 1 cut(s) 690
BseAI TCCGGA 1 cut(s) 818
BseCI ATCGAT 1 cut(s) 80
BseDI CCNNGG 1 cut(s) 508
BseGI GGATG 4 cut(s) 52, 56, 442, 906
BseLI CCNNNNNNNGG 3 cut(s) 585, 720, 783
BseMI GCAATG 1 cut(s) 690
BseMII CTCAG 1 cut(s) 977
BseNI ACTGG 1 cut(s) 866
BseXI GCAGC 3 cut(s) 361, 541, 773
BsgI GTGCAG 2 cut(s) 780, 932
Bsh1285I CGRYCG 1 cut(s) 241
BshFI GGCC 1 cut(s) 969
BshVI ATCGAT 1 cut(s) 80
BsiEI CGRYCG 1 cut(s) 241
BsiHKCI CYCGRG 1 cut(s) 697
BsiSI CCGG 2 cut(s) 366, 819
BslFI GGGAC 1 cut(s) 248
BslI CCNNNNNNNGG 3 cut(s) 585, 720, 783
BsmFI GGGAC 1 cut(s) 248
BsmI GAATGC 1 cut(s) 986
BsnI GGCC 1 cut(s) 969
BsoBI CYCGRG 1 cut(s) 697
Bsp13I TCCGGA 1 cut(s) 818
Bsp143I GATC 2 cut(s) 81, 927
BspACI CCGC 2 cut(s) 197, 955
BspANI GGCC 1 cut(s) 969
BspCNI CTCAG 1 cut(s) 976
BspDI ATCGAT 1 cut(s) 80
BspEI TCCGGA 1 cut(s) 818
BspQI GCTCTTC 2 cut(s) 38, 668
BsrDI GCAATG 1 cut(s) 690
BsrI ACTGG 1 cut(s) 866
BssECI CCNNGG 1 cut(s) 508
BssMI GATC 2 cut(s) 81, 927
BssT1I CCWWGG 1 cut(s) 508
Bst4CI ACNGT 4 cut(s) 239, 263, 385, 884
Bst6I CTCTTC 5 cut(s) 38, 43, 79, 171, 668
BstC8I GCNNGC 2 cut(s) 6, 805
BstDEI CTNAG 2 cut(s) 963, 993
BstEII GGTNACC 2 cut(s) 187, 473
BstENI CCTNNNNNAGG 1 cut(s) 781
BstF5I GGATG 4 cut(s) 52, 56, 442, 906
BstHHI GCGC 2 cut(s) 600, 944
BstKTI GATC 2 cut(s) 84, 930
BstMBI GATC 2 cut(s) 81, 927
BstMCI CGRYCG 1 cut(s) 241
BstMWI GCNNNNNNNGC 1 cut(s) 355
BstNSI RCATGY 2 cut(s) 8, 349
BstPI GGTNACC 2 cut(s) 187, 473
BstSFI CTRYAG 1 cut(s) 975
BstV1I GCAGC 3 cut(s) 361, 541, 773
BstV2I GAAGAC 3 cut(s) 417, 752, 807
Bsu15I ATCGAT 1 cut(s) 80
Bsu36I CCTNAGG 1 cut(s) 963
BsuRI GGCC 1 cut(s) 969
BsuTUI ATCGAT 1 cut(s) 80
BtsCI GGATG 4 cut(s) 52, 56, 442, 906
BtsI GCAGTG 1 cut(s) 771
BtsIMutI CAGTG 1 cut(s) 771
Cac8I GCNNGC 2 cut(s) 6, 805
CfoI GCGC 2 cut(s) 600, 944
Cfr13I GGNCC 1 cut(s) 967
ClaI ATCGAT 1 cut(s) 80
Csp6I GTAC 1 cut(s) 885
CspCI CAANNNNNGTGG 2 cut(s) 451, 486
CviAII CATG 7 cut(s) 5, 28, 127, 184, 228, 346, 925
CviQI GTAC 1 cut(s) 885
DdeI CTNAG 2 cut(s) 963, 993
DpnI GATC 2 cut(s) 83, 929
DpnII GATC 2 cut(s) 81, 927
Eam1104I CTCTTC 5 cut(s) 38, 43, 79, 171, 668
EarI CTCTTC 5 cut(s) 38, 43, 79, 171, 668
Eco130I CCWWGG 1 cut(s) 508
Eco57I CTGAAG 3 cut(s) 126, 487, 819
Eco81I CCTNAGG 1 cut(s) 963
Eco88I CYCGRG 1 cut(s) 697
Eco91I GGTNACC 2 cut(s) 187, 473
EcoNI CCTNNNNNAGG 1 cut(s) 781
EcoO109I RGGNCCY 1 cut(s) 967
EcoO65I GGTNACC 2 cut(s) 187, 473
EcoT14I CCWWGG 1 cut(s) 508
EcoT22I ATGCAT 1 cut(s) 6
ErhI CCWWGG 1 cut(s) 508
FaeI CATG 7 cut(s) 8, 31, 130, 187, 231, 349, 928
FalI AAGNNNNNCTT 2 cut(s) 402, 434
FaqI GGGAC 1 cut(s) 248
FatI CATG 7 cut(s) 4, 27, 126, 183, 227, 345, 924
FauI CCCGC 1 cut(s) 190
FblI GTMKAC 1 cut(s) 428
Fnu4HI GCNGC 3 cut(s) 350, 530, 762
FokI GGATG 4 cut(s) 39, 43, 449, 913
Fsp4HI GCNGC 3 cut(s) 350, 530, 762
FspBI CTAG 1 cut(s) 843
FspI TGCGCA 1 cut(s) 943
GlaI GCGC 2 cut(s) 599, 943
GluI GCNGC 3 cut(s) 350, 530, 762
HaeIII GGCC 1 cut(s) 969
HapII CCGG 2 cut(s) 366, 819
HhaI GCGC 2 cut(s) 600, 944
Hin1II CATG 7 cut(s) 8, 31, 130, 187, 231, 349, 928
Hin6I GCGC 2 cut(s) 598, 942
HinP1I GCGC 2 cut(s) 598, 942
HincII GTYRAC 1 cut(s) 266
HindII GTYRAC 1 cut(s) 266
HindIII AAGCTT 2 cut(s) 356, 801
HinfI GANTC 5 cut(s) 97, 311, 589, 643, 650
HpaII CCGG 2 cut(s) 366, 819
HphI GGTGA 3 cut(s) 55, 485, 530
Hpy166II GTNNAC 4 cut(s) 266, 429, 737, 771
Hpy188I TCNGA 1 cut(s) 409
Hpy188III TCNNGA 6 cut(s) 586, 640, 647, 670, 819, 893
Hpy8I GTNNAC 4 cut(s) 266, 429, 737, 771
Hpy99I CGWCG 1 cut(s) 263
HpyAV CCTTC 2 cut(s) 70, 76
HpyCH4III ACNGT 4 cut(s) 239, 263, 385, 884
HpyCH4V TGCA 7 cut(s) 4, 349, 761, 807, 834, 913, 984
HpyF10VI GCNNNNNNNGC 1 cut(s) 355
HpyF3I CTNAG 2 cut(s) 963, 993
Hsp92II CATG 7 cut(s) 8, 31, 130, 187, 231, 349, 928
HspAI GCGC 2 cut(s) 598, 942
Kpn2I TCCGGA 1 cut(s) 818
Kzo9I GATC 2 cut(s) 81, 927
LguI GCTCTTC 2 cut(s) 38, 668
LmnI GCTCC 2 cut(s) 769, 821
Lsp1109I GCAGC 3 cut(s) 361, 541, 773
LweI GCATC 1 cut(s) 833
MaeI CTAG 1 cut(s) 843
MaeIII GTNAC 4 cut(s) 187, 200, 385, 473
MalI GATC 2 cut(s) 83, 929
MboI GATC 2 cut(s) 81, 927
MluCI AATT 3 cut(s) 533, 787, 934
MlyI GAGTC 1 cut(s) 659
MnlI CCTC 9 cut(s) 111, 174, 202, 304, 551, 725, 787, 972, 980
Mph1103I ATGCAT 1 cut(s) 6
MroI TCCGGA 1 cut(s) 818
MseI TTAA 1 cut(s) 219
MspI CCGG 2 cut(s) 366, 819
Mva1269I GAATGC 1 cut(s) 986
MwoI GCNNNNNNNGC 1 cut(s) 355
NdeII GATC 2 cut(s) 81, 927
NlaIII CATG 7 cut(s) 8, 31, 130, 187, 231, 349, 928
NmuCI GTSAC 2 cut(s) 385, 473
NsbI TGCGCA 1 cut(s) 943
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 2 cut(s) 8, 349
PaeI GCATGC 1 cut(s) 8
PaeR7I CTCGAG 1 cut(s) 697
PciSI GCTCTTC 2 cut(s) 38, 668
PctI GAATGC 1 cut(s) 986
PfeI GAWTC 4 cut(s) 97, 311, 589, 643
PflMI CCANNNNNTGG 1 cut(s) 585
PkrI GCNGC 3 cut(s) 351, 531, 763
PleI GAGTC 1 cut(s) 658
PpsI GAGTC 1 cut(s) 658
PspEI GGTNACC 2 cut(s) 187, 473
PspPI GGNCC 1 cut(s) 967
PspXI VCTCGAGB 1 cut(s) 697
RsaI GTAC 1 cut(s) 886
RsaNI GTAC 1 cut(s) 885
SapI GCTCTTC 2 cut(s) 38, 668
SaqAI TTAA 1 cut(s) 219
SatI GCNGC 3 cut(s) 350, 530, 762
Sau3AI GATC 2 cut(s) 81, 927
Sau96I GGNCC 1 cut(s) 967
SchI GAGTC 1 cut(s) 659
SfaNI GCATC 1 cut(s) 833
SfcI CTRYAG 1 cut(s) 975
Sfr274I CTCGAG 1 cut(s) 697
SlaI CTCGAG 1 cut(s) 697
SmlI CTYRAG 3 cut(s) 353, 466, 697
SmoI CTYRAG 3 cut(s) 353, 466, 697
SpeI ACTAGT 1 cut(s) 842
SphI GCATGC 1 cut(s) 8
Sse9I AATT 3 cut(s) 533, 787, 934
SsiI CCGC 2 cut(s) 197, 955
SspMI CTAG 1 cut(s) 843
StyI CCWWGG 1 cut(s) 508
TaaI ACNGT 4 cut(s) 239, 263, 385, 884
TaqI TCGA 6 cut(s) 80, 241, 285, 549, 653, 698
TasI AATT 3 cut(s) 533, 787, 934
TatI WGTACW 1 cut(s) 884
TfiI GAWTC 4 cut(s) 97, 311, 589, 643
Tru1I TTAA 1 cut(s) 219
Tru9I TTAA 1 cut(s) 219
TscAI CASTG 1 cut(s) 778
TseFI GTSAC 2 cut(s) 385, 473
TseI GCWGC 3 cut(s) 349, 529, 761
Tsp45I GTSAC 2 cut(s) 385, 473
TspDTI ATGAA 5 cut(s) 66, 98, 115, 172, 917
TspGWI ACGGA 1 cut(s) 756
TspRI CASTG 1 cut(s) 778
Van91I CCANNNNNTGG 1 cut(s) 585
XagI CCTNNNNNAGG 1 cut(s) 781
XapI RAATTY 1 cut(s) 787
XceI RCATGY 2 cut(s) 8, 349
XhoI CTCGAG 1 cut(s) 697
XmiI GTMKAC 1 cut(s) 428
XspI CTAG 1 cut(s) 843
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.