Rroxscaffold_5G00347630

Catalyzes xyloglucan endohydrolysis (XEH) and or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0000460
Physical Location & Seq
Forward (+)
19192731 .. 19195056
2326 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00347630.1

Sequence Viewer

Length: 885 bp
ATGGCTAGCAGATACTGCCTGGTGTTTTTGCTCACTATTATCATTGGTTCTTTGATGGTTGTTATCTCTTCTGCTAGTAATTTCAATCAAGATTTTGAGATTACATGGGGTGAGGGCCGAGCTAAGATACTAAACAACAACCAGCTTCTTATACTTTCCCTCGACAGAATCTCAGGGTCAGGATTCCAGTCCAAGAATGAGTATCTATTTGGTAAGATATCGATGCAACTCAAGCTTGTCCCAGGAAACTCCGCTGGCACTGTCACCGCCTATTATTTGTCCTCAAAAGGGTCAGCATGGGATGAGATAGATTTCGAGTTCTTGGGTAACTTGAGTGGTGATCCGTACACCGTCCACACCAATGTGTATAGCCAAGGCAAGGGCGATAGAGAGCAACAGTTCCATCTCTGGTTTGACCCAACCACCGACTTTCATACATATTCGATTCTTTGGAACCCTCAGCGCATTGTATTCTATGTTGATGGCACCCCAATAAGAGAGTTCAAGAACTTGGAGTCAATTGGTGTTCCATTCCCTGAGGACCAGCCCATGCGATTACACTCAAGCCTTTGGAGTGCTGATGACTGGGCTACAAGGGGTGGTCTAGTTAAAACAGACTGGACCAAAGCTCCTTTCATTGCTTCTTATAGGAACTTCAATGCCAATGCTTGTATATGGTCCTCCGGAACATCTTCTTGCACTTCAAAATCCCCATCGTCTGGTGGATGGCTCTCACAAGAGTTGGATTTAGGAAGTAAGAAAAAATTGAAATGGGTGCAAGAGAAGTTTATGATCTACAATTACTGCAGAGACACAAAAAGAGGTTTCCCCAAGGCTTCCCTCAAGAATGCAGAGTGTCCTAAGAAATTAGACGATAAACTTTGA

Protein Analysis

294

Amino Acids

33.24

Weight (kDa)

8.47

Isoelectric Point (pI)

31.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_16 PF00722 28 - 209 2.9e-67 Glycosyl hydrolases family 16
XET_C PF06955 239 - 278 3.6e-13 Xyloglucan endo-transglycosylase (XET) C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000460)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G25810 AT4G30270 AT5G57550 AT5G57560
fragaria_vesca FvH4_3g00833 FvH4_3g00840 FvH4_3g00850 FvH4_4g06700 FvH4_4g09230
malus_domestica MD13G1237300.v1.1 MD13G1268000.v1.1 MD13G1268900.v1.1 MD16G1267200.v1.1 MD16G1267300.v1.1
prunus_persica Prupe.1G069800_v2.0.a1 Prupe.1G088600_v2.0.a1 Prupe.1G088800_v2.0.a1 Prupe.1G088900_v2.0.a1 Prupe.1G089000_v2.0.a1
pyrus_communis pycom13g21020 pycom13g22350 pycom13g22380 pycom16g23630 pycom16g23640
rosa_chinensis RchiOBHm_Chr4g0398631 RchiOBHm_Chr4g0402891 RchiOBHm_Chr4g0402911 RchiOBHm_Chr4g0402921 RchiOBHm_Chr4g0402931 RchiOBHm_Chr4g0402951 RchiOBHm_Chr4g0402961 RchiOBHm_Chr4g0402981
rosa_laevigata RLG00000009031 RLG00000009033 RLG00000009034 RLG00000009035 RLG00000009039 RLG00000009040 RLG00000009339
rosa_multiflora Rmu_co8186292.1_g000001 Rmu_sc0000990.1_g000004 Rmu_sc0001023.1_g000002 Rmu_sc0001023.1_g000004 Rmu_sc0001023.1_g000007 Rmu_sc0001023.1_g000015 Rmu_sc0002178.1_g000007 Rmu_sc0002451.1_g000069 Rmu_sc0002451.1_g000079 Rmu_sc0002451.1_g000080 Rmu_sc0008058.1_g000026 Rmu_sc0008058.1_g000029 Rmu_sc0008058.1_g000030
rosa_roxburghii Rroxscaffold_5G00343590 Rroxscaffold_5G00347620 Rroxscaffold_5G00347630 Rroxscaffold_5G00347640 Rroxscaffold_5G00347650 Rroxscaffold_5G00347660 Rroxscaffold_5G00347670 Rroxscaffold_5G00347690
rosa_rugosa Rorug04G0009900 Rorug04G0039000 Rorug04G0039100 Rorug04G0039200 Rorug04G0039300 Rorug04G0039400 Rorug04G0039500 Rorug04G0039600 Rorug04G0039600 Rorug04G0040200
rosa_samantha Rh4AG087800 Rh4AG116500 Rh4AG116700 Rh4AG116800 Rh4AG116900 Rh4AG117000 Rh4AG117100 Rh4BG109300 Rh4BG109400 Rh4BG109500 Rh4BG109600 Rh4BG109700 Rh4BG109800 Rh4CG096100 Rh4CG123700 Rh4CG124200
rosa_wichuraiana Rw4G007280 Rw4G009320 Rw4G009340 Rw4G009370 Rw4G009380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 485
AccB7I CCANNNNNTGG 1 cut(s) 719
AccIII TCCGGA 1 cut(s) 683
AciI CCGC 2 cut(s) 252, 267
AclWI GGATC 1 cut(s) 335
AfaI GTAC 1 cut(s) 347
AfiI CCNNNNNNNGG 3 cut(s) 288, 379, 719
AgsI TTSAA 5 cut(s) 85, 505, 658, 705, 769
AjnI CCWGG 2 cut(s) 18, 241
AjuI GAANNNNNNNTTGG 2 cut(s) 617, 649
AleI CACNNNNGTG 1 cut(s) 362
AluBI AGCT 4 cut(s) 122, 145, 235, 629
AluI AGCT 4 cut(s) 122, 145, 235, 629
Alw26I GTCTC 1 cut(s) 804
AlwI GGATC 1 cut(s) 335
AlwNI CAGNNNCTG 1 cut(s) 15
Aor13HI TCCGGA 1 cut(s) 683
AoxI GGCC 1 cut(s) 115
Asp700I GAANNNNTTC 1 cut(s) 691
AspLEI GCGC 1 cut(s) 465
AspS9I GGNCC 4 cut(s) 115, 541, 621, 678
AsuHPI GGTGA 3 cut(s) 122, 256, 350
AsuNHI GCTAGC 1 cut(s) 5
AvaII GGWCC 3 cut(s) 541, 621, 678
AxyI CCTNAGG 1 cut(s) 537
BanI GGYRCC 1 cut(s) 485
BbvCI CCTCAGC 1 cut(s) 459
BccI CCATC 5 cut(s) 49, 411, 476, 720, 721
BciT130I CCWGG 2 cut(s) 20, 243
BcoDI GTCTC 1 cut(s) 804
BfaI CTAG 3 cut(s) 6, 75, 605
BfmI CTRYAG 1 cut(s) 805
Bme1390I CCNGG 2 cut(s) 20, 243
Bme18I GGWCC 3 cut(s) 541, 621, 678
BmgT120I GGNCC 4 cut(s) 115, 541, 621, 678
BmiI GGNNCC 2 cut(s) 455, 487
BmrFI CCNGG 2 cut(s) 20, 243
BmrI ACTGGG 1 cut(s) 595
BmsI GCATC 1 cut(s) 213
BmtI GCTAGC 1 cut(s) 9
BmuI ACTGGG 1 cut(s) 595
Bpu10I CCTNAGC 1 cut(s) 459
BpuEI CTTGAG 4 cut(s) 215, 352, 547, 827
Bsa29I ATCGAT 1 cut(s) 221
BsaJI CCNNGG 3 cut(s) 241, 373, 831
BsaWI WCCGGW 1 cut(s) 683
BsaXI ACNNNNNCTCC 2 cut(s) 613, 643
Bsc4I CCNNNNNNNGG 3 cut(s) 288, 379, 719
Bse1I ACTGG 3 cut(s) 187, 590, 623
Bse21I CCTNAGG 1 cut(s) 537
Bse3DI GCAATG 1 cut(s) 636
BseAI TCCGGA 1 cut(s) 683
BseBI CCWGG 2 cut(s) 20, 243
BseCI ATCGAT 1 cut(s) 221
BseDI CCNNGG 3 cut(s) 241, 373, 831
BseGI GGATG 2 cut(s) 307, 731
BseLI CCNNNNNNNGG 3 cut(s) 288, 379, 719
BseMI GCAATG 1 cut(s) 636
BseMII CTCAG 3 cut(s) 186, 473, 528
BseNI ACTGG 3 cut(s) 187, 590, 623
BshFI GGCC 1 cut(s) 117
BshNI GGYRCC 1 cut(s) 485
BshVI ATCGAT 1 cut(s) 221
BsiSI CCGG 1 cut(s) 684
BslFI GGGAC 1 cut(s) 224
BslI CCNNNNNNNGG 3 cut(s) 288, 379, 719
BsmAI GTCTC 1 cut(s) 804
BsmFI GGGAC 1 cut(s) 224
BsmI GAATGC 1 cut(s) 853
BsnI GGCC 1 cut(s) 117
Bsp13I TCCGGA 1 cut(s) 683
Bsp143I GATC 2 cut(s) 340, 792
BspACI CCGC 2 cut(s) 252, 267
BspANI GGCC 1 cut(s) 117
BspCNI CTCAG 3 cut(s) 185, 472, 529
BspDI ATCGAT 1 cut(s) 221
BspEI TCCGGA 1 cut(s) 683
BspLI GGNNCC 2 cut(s) 455, 487
BspMAI CTGCAG 1 cut(s) 809
BspOI GCTAGC 1 cut(s) 9
BspPI GGATC 1 cut(s) 335
BspT107I GGYRCC 1 cut(s) 485
BsrDI GCAATG 1 cut(s) 636
BsrI ACTGG 3 cut(s) 187, 590, 623
BssECI CCNNGG 3 cut(s) 241, 373, 831
BssMI GATC 2 cut(s) 340, 792
BssT1I CCWWGG 2 cut(s) 373, 831
Bst2UI CCWGG 2 cut(s) 20, 243
Bst4CI ACNGT 3 cut(s) 262, 352, 399
Bst6I CTCTTC 1 cut(s) 73
BstAPI GCANNNNNTGC 1 cut(s) 15
BstC8I GCNNGC 2 cut(s) 7, 256
BstDEI CTNAG 5 cut(s) 123, 172, 459, 537, 861
BstF5I GGATG 2 cut(s) 307, 731
BstHHI GCGC 1 cut(s) 465
BstKTI GATC 2 cut(s) 343, 795
BstMAI GTCTC 1 cut(s) 804
BstMBI GATC 2 cut(s) 340, 792
BstMWI GCNNNNNNNGC 2 cut(s) 15, 232
BstNI CCWGG 2 cut(s) 20, 243
BstSCI CCNGG 2 cut(s) 18, 241
BstSFI CTRYAG 1 cut(s) 805
Bsu15I ATCGAT 1 cut(s) 221
Bsu36I CCTNAGG 1 cut(s) 537
BsuRI GGCC 1 cut(s) 117
BsuTUI ATCGAT 1 cut(s) 221
BtsCI GGATG 2 cut(s) 307, 731
BtsIMutI CAGTG 1 cut(s) 258
Cac8I GCNNGC 2 cut(s) 7, 256
CaiI CAGNNNCTG 1 cut(s) 15
CfoI GCGC 1 cut(s) 465
Cfr13I GGNCC 4 cut(s) 115, 541, 621, 678
ClaI ATCGAT 1 cut(s) 221
Csp6I GTAC 1 cut(s) 346
CviAII CATG 3 cut(s) 105, 297, 550
CviQI GTAC 1 cut(s) 346
DdeI CTNAG 5 cut(s) 123, 172, 459, 537, 861
DpnI GATC 2 cut(s) 342, 794
DpnII GATC 2 cut(s) 340, 792
Eam1104I CTCTTC 1 cut(s) 73
EarI CTCTTC 1 cut(s) 73
Eco130I CCWWGG 2 cut(s) 373, 831
Eco32I GATATC 1 cut(s) 219
Eco47I GGWCC 3 cut(s) 541, 621, 678
Eco81I CCTNAGG 1 cut(s) 537
EcoRII CCWGG 2 cut(s) 18, 241
EcoRV GATATC 1 cut(s) 219
EcoT14I CCWWGG 2 cut(s) 373, 831
ErhI CCWWGG 2 cut(s) 373, 831
FaeI CATG 3 cut(s) 108, 300, 553
FaqI GGGAC 1 cut(s) 224
FatI CATG 3 cut(s) 104, 296, 549
FokI GGATG 2 cut(s) 314, 738
FspBI CTAG 3 cut(s) 6, 75, 605
GlaI GCGC 1 cut(s) 464
HaeIII GGCC 1 cut(s) 117
HapII CCGG 1 cut(s) 684
HhaI GCGC 1 cut(s) 465
Hin1II CATG 3 cut(s) 108, 300, 553
Hin6I GCGC 1 cut(s) 463
HinP1I GCGC 1 cut(s) 463
HindIII AAGCTT 1 cut(s) 233
HinfI GANTC 4 cut(s) 168, 183, 445, 515
HpaII CCGG 1 cut(s) 684
HphI GGTGA 3 cut(s) 122, 256, 350
Hpy166II GTNNAC 2 cut(s) 348, 355
Hpy188III TCNNGA 5 cut(s) 89, 180, 505, 684, 844
Hpy8I GTNNAC 2 cut(s) 348, 355
HpyCH4III ACNGT 3 cut(s) 262, 352, 399
HpyCH4V TGCA 5 cut(s) 226, 699, 778, 807, 851
HpyF10VI GCNNNNNNNGC 2 cut(s) 15, 232
HpyF3I CTNAG 5 cut(s) 123, 172, 459, 537, 861
Hsp92II CATG 3 cut(s) 108, 300, 553
HspAI GCGC 1 cut(s) 463
Kpn2I TCCGGA 1 cut(s) 683
Kzo9I GATC 2 cut(s) 340, 792
LmnI GCTCC 1 cut(s) 634
LweI GCATC 1 cut(s) 213
MaeI CTAG 3 cut(s) 6, 75, 605
MaeIII GTNAC 2 cut(s) 262, 326
MalI GATC 2 cut(s) 342, 794
MboI GATC 2 cut(s) 340, 792
MboII GAAGA 2 cut(s) 60, 684
MfeI CAATTG 1 cut(s) 519
MluCI AATT 5 cut(s) 79, 519, 764, 799, 866
MlyI GAGTC 1 cut(s) 524
MmeI TCCRAC 1 cut(s) 723
MnlI CCTC 8 cut(s) 106, 170, 292, 468, 532, 691, 815, 851
MroI TCCGGA 1 cut(s) 683
MroXI GAANNNNTTC 1 cut(s) 691
MseI TTAA 1 cut(s) 609
MslI CAYNNNNRTG 2 cut(s) 360, 362
MspA1I CMGCKG 1 cut(s) 254
MspI CCGG 1 cut(s) 684
MspR9I CCNGG 2 cut(s) 20, 243
MunI CAATTG 1 cut(s) 519
Mva1269I GAATGC 1 cut(s) 853
MvaI CCWGG 2 cut(s) 20, 243
MwoI GCNNNNNNNGC 2 cut(s) 15, 232
NdeII GATC 2 cut(s) 340, 792
NheI GCTAGC 1 cut(s) 5
NlaIII CATG 3 cut(s) 108, 300, 553
NlaIV GGNNCC 2 cut(s) 455, 487
NmeAIII GCCGAG 1 cut(s) 143
NmuCI GTSAC 1 cut(s) 262
OliI CACNNNNGTG 1 cut(s) 362
PctI GAATGC 1 cut(s) 853
PdmI GAANNNNTTC 1 cut(s) 691
PfeI GAWTC 3 cut(s) 168, 183, 445
PflMI CCANNNNNTGG 1 cut(s) 719
PleI GAGTC 1 cut(s) 523
PpsI GAGTC 1 cut(s) 523
Psp6I CCWGG 2 cut(s) 18, 241
PspGI CCWGG 2 cut(s) 18, 241
PspN4I GGNNCC 2 cut(s) 455, 487
PspPI GGNCC 4 cut(s) 115, 541, 621, 678
PstI CTGCAG 1 cut(s) 809
PstNI CAGNNNCTG 1 cut(s) 15
RsaI GTAC 1 cut(s) 347
RsaNI GTAC 1 cut(s) 346
RseI CAYNNNNRTG 2 cut(s) 360, 362
SaqAI TTAA 1 cut(s) 609
Sau3AI GATC 2 cut(s) 340, 792
Sau96I GGNCC 4 cut(s) 115, 541, 621, 678
SchI GAGTC 1 cut(s) 524
ScrFI CCNGG 2 cut(s) 20, 243
SetI ASST 5 cut(s) 124, 147, 237, 631, 826
SfaNI GCATC 1 cut(s) 213
SfcI CTRYAG 1 cut(s) 805
SinI GGWCC 3 cut(s) 541, 621, 678
SmiMI CAYNNNNRTG 2 cut(s) 360, 362
SmlI CTYRAG 4 cut(s) 230, 331, 562, 842
SmoI CTYRAG 4 cut(s) 230, 331, 562, 842
Sse9I AATT 5 cut(s) 79, 519, 764, 799, 866
SsiI CCGC 2 cut(s) 252, 267
SspMI CTAG 3 cut(s) 6, 75, 605
StyD4I CCNGG 2 cut(s) 18, 241
StyI CCWWGG 2 cut(s) 373, 831
TaaI ACNGT 3 cut(s) 262, 352, 399
TaqI TCGA 4 cut(s) 162, 221, 315, 443
TasI AATT 5 cut(s) 79, 519, 764, 799, 866
TfiI GAWTC 3 cut(s) 168, 183, 445
Tru1I TTAA 1 cut(s) 609
Tru9I TTAA 1 cut(s) 609
TscAI CASTG 1 cut(s) 265
TseFI GTSAC 1 cut(s) 262
Tsp45I GTSAC 1 cut(s) 262
TspDTI ATGAA 2 cut(s) 422, 625
TspGWI ACGGA 1 cut(s) 333
TspRI CASTG 1 cut(s) 265
Van91I CCANNNNNTGG 1 cut(s) 719
VpaK11BI GGWCC 3 cut(s) 541, 621, 678
XmnI GAANNNNTTC 1 cut(s) 691
XspI CTAG 3 cut(s) 6, 75, 605
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.