MD16G1267300.v1.1

Catalyzes xyloglucan endohydrolysis (XEH) and or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0000460
Physical Location & Seq
Forward (+)
34168561 .. 34169381
821 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1267300.v1.1.491

Sequence Viewer

Length: 573 bp
ATGCAGCTCAAGCTTGTCCCTGGAAACTCCGCTGGCACCGTCACAGCCTACTACATAGACTTTGAGTTCTTGGGGAACTTGAGTGGAGACCCTTACATTCTTCACACCAATGTCTTCAGCCAAGGCAAAGGCAACAGAGAGCTGCAATTCTACCTCTGGTTTGACCCAATTGCCGACTTCCACACATATACCATCCTCTGGAACTCCCAGCGCATTATATTCTCAGTAGATGGAACTCCCATCAGAGAGTTCAAGAACCAAGAGTCGAACGACGTTCCGTTTCCAAAAAGCCAGCCGATGAGGATATACTCGAGCCTGTGGAATGCCGATGATTGGGCAACGAGGGGAGGACTTGTGAAGACCGATTGGAGCCAAGCTCCTTTCGCCGCCTCACAGGAATTTCATTCTGCAACTTCAAGTAATGCTAACAATGGAGCTTGGCTTTCTCAAGACTTGGACTCTATAAGCCAAGACAGGCTGAAATGGGTGCAGACCAACTACATGATTTACAATTACTGTGCTGACACCAAGAGATTTCCCCAAGGCCTCCCGGTGGAATGCACCGCTTCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
Glyco_hydro_16 XET_C

Protein Analysis

191

Amino Acids

21.66

Weight (kDa)

5.07

Isoelectric Point (pI)

39.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_16 PF00722 2 - 124 4.5e-42 Glycosyl hydrolases family 16
XET_C PF06955 143 - 187 1.7e-20 Xyloglucan endo-transglycosylase (XET) C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000460)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G25810 AT4G30270 AT5G57550 AT5G57560
fragaria_vesca FvH4_3g00833 FvH4_3g00840 FvH4_3g00850 FvH4_4g06700 FvH4_4g09230
malus_domestica MD13G1237300.v1.1 MD13G1268000.v1.1 MD13G1268900.v1.1 MD16G1267200.v1.1 MD16G1267300.v1.1
prunus_persica Prupe.1G069800_v2.0.a1 Prupe.1G088600_v2.0.a1 Prupe.1G088800_v2.0.a1 Prupe.1G088900_v2.0.a1 Prupe.1G089000_v2.0.a1
pyrus_communis pycom13g21020 pycom13g22350 pycom13g22380 pycom16g23630 pycom16g23640
rosa_chinensis RchiOBHm_Chr4g0398631 RchiOBHm_Chr4g0402891 RchiOBHm_Chr4g0402911 RchiOBHm_Chr4g0402921 RchiOBHm_Chr4g0402931 RchiOBHm_Chr4g0402951 RchiOBHm_Chr4g0402961 RchiOBHm_Chr4g0402981
rosa_laevigata RLG00000009031 RLG00000009033 RLG00000009034 RLG00000009035 RLG00000009039 RLG00000009040 RLG00000009339
rosa_multiflora Rmu_co8186292.1_g000001 Rmu_sc0000990.1_g000004 Rmu_sc0001023.1_g000002 Rmu_sc0001023.1_g000004 Rmu_sc0001023.1_g000007 Rmu_sc0001023.1_g000015 Rmu_sc0002178.1_g000007 Rmu_sc0002451.1_g000069 Rmu_sc0002451.1_g000079 Rmu_sc0002451.1_g000080 Rmu_sc0008058.1_g000026 Rmu_sc0008058.1_g000029 Rmu_sc0008058.1_g000030
rosa_roxburghii Rroxscaffold_5G00343590 Rroxscaffold_5G00347620 Rroxscaffold_5G00347630 Rroxscaffold_5G00347640 Rroxscaffold_5G00347650 Rroxscaffold_5G00347660 Rroxscaffold_5G00347670 Rroxscaffold_5G00347690
rosa_rugosa Rorug04G0009900 Rorug04G0039000 Rorug04G0039100 Rorug04G0039200 Rorug04G0039300 Rorug04G0039400 Rorug04G0039500 Rorug04G0039600 Rorug04G0039600 Rorug04G0040200
rosa_samantha Rh4AG087800 Rh4AG116500 Rh4AG116700 Rh4AG116800 Rh4AG116900 Rh4AG117000 Rh4AG117100 Rh4BG109300 Rh4BG109400 Rh4BG109500 Rh4BG109600 Rh4BG109700 Rh4BG109800 Rh4CG096100 Rh4CG123700 Rh4CG124200
rosa_wichuraiana Rw4G007280 Rw4G009320 Rw4G009340 Rw4G009370 Rw4G009380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 35
AccB7I CCANNNNNTGG 1 cut(s) 198
AciI CCGC 3 cut(s) 30, 387, 564
AcsI RAATTY 1 cut(s) 398
AcuI CTGAAG 1 cut(s) 100
AfiI CCNNNNNNNGG 3 cut(s) 198, 333, 553
AgsI TTSAA 2 cut(s) 253, 417
AjnI CCWGG 1 cut(s) 19
AluBI AGCT 5 cut(s) 7, 13, 142, 377, 437
AluI AGCT 5 cut(s) 7, 13, 142, 377, 437
Alw26I GTCTC 1 cut(s) 81
Ama87I CYCGRG 1 cut(s) 310
AoxI GGCC 1 cut(s) 544
ApeKI GCWGC 2 cut(s) 4, 142
ApoI RAATTY 1 cut(s) 398
AspLEI GCGC 1 cut(s) 213
AsuC2I CCSGG 1 cut(s) 551
AvaI CYCGRG 1 cut(s) 310
BanI GGYRCC 1 cut(s) 35
BbsI GAAGAC 2 cut(s) 106, 365
BbvI GCAGC 2 cut(s) 16, 129
BccI CCATC 3 cut(s) 200, 224, 248
BciT130I CCWGG 1 cut(s) 21
BcnI CCSGG 1 cut(s) 551
BcoDI GTCTC 1 cut(s) 81
BisI GCNGC 3 cut(s) 5, 143, 387
BlsI GCNGC 3 cut(s) 6, 144, 388
Bme1390I CCNGG 2 cut(s) 21, 551
BmeT110I CYCGRG 1 cut(s) 310
BmiI GGNNCC 2 cut(s) 37, 371
BmrFI CCNGG 2 cut(s) 21, 551
BpiI GAAGAC 2 cut(s) 106, 365
BplI GAGNNNNNCTC 2 cut(s) 361, 393
BpuEI CTTGAG 2 cut(s) 100, 432
BpuMI CCSGG 1 cut(s) 551
BsaI GGTCTC 1 cut(s) 81
BsaJI CCNNGG 3 cut(s) 19, 121, 541
BsaXI ACNNNNNCTCC 2 cut(s) 339, 369
Bsc4I CCNNNNNNNGG 3 cut(s) 198, 333, 553
BseBI CCWGG 1 cut(s) 21
BseDI CCNNGG 3 cut(s) 19, 121, 541
BseGI GGATG 1 cut(s) 192
BseLI CCNNNNNNNGG 3 cut(s) 198, 333, 553
BseMII CTCAG 1 cut(s) 237
BseXI GCAGC 2 cut(s) 16, 129
BseYI CCCAGC 1 cut(s) 207
BsgI GTGCAG 1 cut(s) 509
BshFI GGCC 1 cut(s) 546
BshNI GGYRCC 1 cut(s) 35
BsiHKCI CYCGRG 1 cut(s) 310
BsiSI CCGG 1 cut(s) 551
BslFI GGGAC 1 cut(s) 2
BslI CCNNNNNNNGG 3 cut(s) 198, 333, 553
BsmAI GTCTC 1 cut(s) 81
BsmFI GGGAC 1 cut(s) 2
BsmI GAATGC 2 cut(s) 328, 563
BsnI GGCC 1 cut(s) 546
Bso31I GGTCTC 1 cut(s) 81
BsoBI CYCGRG 1 cut(s) 310
BspACI CCGC 3 cut(s) 30, 387, 564
BspANI GGCC 1 cut(s) 546
BspCNI CTCAG 1 cut(s) 236
BspLI GGNNCC 2 cut(s) 37, 371
BspT107I GGYRCC 1 cut(s) 35
BspTNI GGTCTC 1 cut(s) 81
BssECI CCNNGG 3 cut(s) 19, 121, 541
BssT1I CCWWGG 2 cut(s) 121, 541
Bst2UI CCWGG 1 cut(s) 21
Bst4CI ACNGT 2 cut(s) 40, 518
BstC8I GCNNGC 2 cut(s) 34, 293
BstDEI CTNAG 1 cut(s) 223
BstF5I GGATG 1 cut(s) 192
BstHHI GCGC 1 cut(s) 213
BstMAI GTCTC 1 cut(s) 81
BstMWI GCNNNNNNNGC 2 cut(s) 10, 383
BstNI CCWGG 1 cut(s) 21
BstSCI CCNGG 2 cut(s) 19, 549
BstV1I GCAGC 2 cut(s) 16, 129
BstV2I GAAGAC 2 cut(s) 106, 365
BsuRI GGCC 1 cut(s) 546
BtsCI GGATG 1 cut(s) 192
Cac8I GCNNGC 2 cut(s) 34, 293
CfoI GCGC 1 cut(s) 213
CviAII CATG 1 cut(s) 502
DdeI CTNAG 1 cut(s) 223
Eco130I CCWWGG 2 cut(s) 121, 541
Eco147I AGGCCT 1 cut(s) 546
Eco31I GGTCTC 1 cut(s) 81
Eco57I CTGAAG 1 cut(s) 100
Eco88I CYCGRG 1 cut(s) 310
EcoRII CCWGG 1 cut(s) 19
EcoT14I CCWWGG 2 cut(s) 121, 541
ErhI CCWWGG 2 cut(s) 121, 541
FaeI CATG 1 cut(s) 505
FaiI YATR 8 cut(s) 56, 187, 189, 218, 307, 464, 503, 571
FaqI GGGAC 1 cut(s) 2
FatI CATG 1 cut(s) 501
Fnu4HI GCNGC 3 cut(s) 5, 143, 387
FokI GGATG 1 cut(s) 179
Fsp4HI GCNGC 3 cut(s) 5, 143, 387
GlaI GCGC 1 cut(s) 212
GluI GCNGC 3 cut(s) 5, 143, 387
GsaI CCCAGC 1 cut(s) 211
HaeIII GGCC 1 cut(s) 546
HapII CCGG 1 cut(s) 551
HhaI GCGC 1 cut(s) 213
Hin1II CATG 1 cut(s) 505
Hin6I GCGC 1 cut(s) 211
HinP1I GCGC 1 cut(s) 211
HindIII AAGCTT 1 cut(s) 11
HinfI GANTC 2 cut(s) 263, 458
HpaII CCGG 1 cut(s) 551
Hpy188I TCNGA 1 cut(s) 245
Hpy188III TCNNGA 3 cut(s) 199, 253, 449
Hpy99I CGWCG 1 cut(s) 275
HpyCH4III ACNGT 2 cut(s) 40, 518
HpyCH4IV ACGT 1 cut(s) 273
HpyCH4V TGCA 5 cut(s) 4, 145, 410, 490, 561
HpyF10VI GCNNNNNNNGC 2 cut(s) 10, 383
HpyF3I CTNAG 1 cut(s) 223
HpySE526I ACGT 1 cut(s) 273
Hsp92II CATG 1 cut(s) 505
HspAI GCGC 1 cut(s) 211
LmnI GCTCC 3 cut(s) 369, 382, 434
Lsp1109I GCAGC 2 cut(s) 16, 129
MaeII ACGT 1 cut(s) 273
MaeIII GTNAC 1 cut(s) 40
MboII GAAGA 3 cut(s) 92, 106, 370
MfeI CAATTG 1 cut(s) 168
MluCI AATT 4 cut(s) 146, 168, 398, 511
MlyI GAGTC 2 cut(s) 272, 452
MnlI CCTC 7 cut(s) 164, 206, 294, 336, 341, 400, 557
MslI CAYNNNNRTG 1 cut(s) 108
MspA1I CMGCKG 1 cut(s) 32
MspI CCGG 1 cut(s) 551
MspR9I CCNGG 2 cut(s) 21, 551
MunI CAATTG 1 cut(s) 168
Mva1269I GAATGC 2 cut(s) 328, 563
MvaI CCWGG 1 cut(s) 21
MwoI GCNNNNNNNGC 2 cut(s) 10, 383
NciI CCSGG 1 cut(s) 551
NlaIII CATG 1 cut(s) 505
NlaIV GGNNCC 2 cut(s) 37, 371
NmuCI GTSAC 1 cut(s) 40
PaeR7I CTCGAG 1 cut(s) 310
PceI AGGCCT 1 cut(s) 546
PctI GAATGC 2 cut(s) 328, 563
PflMI CCANNNNNTGG 1 cut(s) 198
PkrI GCNGC 3 cut(s) 6, 144, 388
PleI GAGTC 2 cut(s) 271, 452
PpsI GAGTC 2 cut(s) 271, 452
Psp6I CCWGG 1 cut(s) 19
PspFI CCCAGC 1 cut(s) 207
PspGI CCWGG 1 cut(s) 19
PspN4I GGNNCC 2 cut(s) 37, 371
PspXI VCTCGAGB 1 cut(s) 310
RseI CAYNNNNRTG 1 cut(s) 108
SatI GCNGC 3 cut(s) 5, 143, 387
SchI GAGTC 2 cut(s) 272, 452
ScrFI CCNGG 2 cut(s) 21, 551
SetI ASST 7 cut(s) 9, 15, 144, 156, 276, 379, 439
Sfr274I CTCGAG 1 cut(s) 310
SlaI CTCGAG 1 cut(s) 310
SmiMI CAYNNNNRTG 1 cut(s) 108
SmlI CTYRAG 4 cut(s) 8, 79, 310, 447
SmoI CTYRAG 4 cut(s) 8, 79, 310, 447
Sse9I AATT 4 cut(s) 146, 168, 398, 511
SseBI AGGCCT 1 cut(s) 546
SsiI CCGC 3 cut(s) 30, 387, 564
StuI AGGCCT 1 cut(s) 546
StyD4I CCNGG 2 cut(s) 19, 549
StyI CCWWGG 2 cut(s) 121, 541
TaaI ACNGT 2 cut(s) 40, 518
TaiI ACGT 1 cut(s) 276
TaqI TCGA 2 cut(s) 266, 311
TaqII GACCGA 1 cut(s) 377
TasI AATT 4 cut(s) 146, 168, 398, 511
TauI GCSGC 1 cut(s) 389
TseFI GTSAC 1 cut(s) 40
TseI GCWGC 2 cut(s) 4, 142
Tsp45I GTSAC 1 cut(s) 40
TspDTI ATGAA 2 cut(s) 392, 558
TspGWI ACGGA 1 cut(s) 267
Van91I CCANNNNNTGG 1 cut(s) 198
XapI RAATTY 1 cut(s) 398
XhoI CTCGAG 1 cut(s) 310
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.