RchiOBHm_Chr4g0402931

Catalyzes xyloglucan endohydrolysis (XEH) and or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0000460
Physical Location & Seq
Reverse (-)
21919023 .. 21919874
852 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ37470

Sequence Viewer

Length: 594 bp
ATGCAGCCCAAGCTTGTCGCCGGAAACTCTGCTGGAACTGTCACAGCCTATTATTTGAAATCAGAAGGGTCTACTTGGGATGAGATAGACTTTGAGTTCTTGGGGAACTTGAGTGGCGACCCAAACATTCTTCACACCAATATCTTCAGCCAAGGCAAAGGAAACCGAAAGCAGCAATTCCACCTCTGGTTTGACCCAACTGCTGATTTCCATGCCTATTTCATTCTCTGGAATCCACAGCGCATTGTTTTTTCAGTTGATGGCACTCCCATTAGAGAGTTCAAGAATCAAGAGTCGAAAGGTGTTCCATTCTTGAAGATCCAAGCAATGAGAGTACACTCGAGCCTCTGGAACGCCGATGATTGGGCAACAAGCGGTGGACTTGTAAAGACAGATTGGACTTGCATTTGGTCTTCTGGAGCATCTTTTTGCAGTTCAACTAGTACTCAAAATGGCGATTGGTTATCACAAGAGCTGGACAGTACAAGTCAAGAAAGGATGAAATGGGTGCAGAACAACTACATGATCTACAATTACTGCGCAGATGTCAAGCGGTTTCCTCAGGGCCTCCATGTAGAATGCAACACTTCTTAG

Protein Analysis

197

Amino Acids

22.44

Weight (kDa)

5.68

Isoelectric Point (pI)

33.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_16 PF00722 6 - 134 4.1e-49 Glycosyl hydrolases family 16
XET_C PF06955 151 - 194 5.2e-20 Xyloglucan endo-transglycosylase (XET) C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000460)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G25810 AT4G30270 AT5G57550 AT5G57560
fragaria_vesca FvH4_3g00833 FvH4_3g00840 FvH4_3g00850 FvH4_4g06700 FvH4_4g09230
malus_domestica MD13G1237300.v1.1 MD13G1268000.v1.1 MD13G1268900.v1.1 MD16G1267200.v1.1 MD16G1267300.v1.1
prunus_persica Prupe.1G069800_v2.0.a1 Prupe.1G088600_v2.0.a1 Prupe.1G088800_v2.0.a1 Prupe.1G088900_v2.0.a1 Prupe.1G089000_v2.0.a1
pyrus_communis pycom13g21020 pycom13g22350 pycom13g22380 pycom16g23630 pycom16g23640
rosa_chinensis RchiOBHm_Chr4g0398631 RchiOBHm_Chr4g0402891 RchiOBHm_Chr4g0402911 RchiOBHm_Chr4g0402921 RchiOBHm_Chr4g0402931 RchiOBHm_Chr4g0402951 RchiOBHm_Chr4g0402961 RchiOBHm_Chr4g0402981
rosa_laevigata RLG00000009031 RLG00000009033 RLG00000009034 RLG00000009035 RLG00000009039 RLG00000009040 RLG00000009339
rosa_multiflora Rmu_co8186292.1_g000001 Rmu_sc0000990.1_g000004 Rmu_sc0001023.1_g000002 Rmu_sc0001023.1_g000004 Rmu_sc0001023.1_g000007 Rmu_sc0001023.1_g000015 Rmu_sc0002178.1_g000007 Rmu_sc0002451.1_g000069 Rmu_sc0002451.1_g000079 Rmu_sc0002451.1_g000080 Rmu_sc0008058.1_g000026 Rmu_sc0008058.1_g000029 Rmu_sc0008058.1_g000030
rosa_roxburghii Rroxscaffold_5G00343590 Rroxscaffold_5G00347620 Rroxscaffold_5G00347630 Rroxscaffold_5G00347640 Rroxscaffold_5G00347650 Rroxscaffold_5G00347660 Rroxscaffold_5G00347670 Rroxscaffold_5G00347690
rosa_rugosa Rorug04G0009900 Rorug04G0039000 Rorug04G0039100 Rorug04G0039200 Rorug04G0039300 Rorug04G0039400 Rorug04G0039500 Rorug04G0039600 Rorug04G0039600 Rorug04G0040200
rosa_samantha Rh4AG087800 Rh4AG116500 Rh4AG116700 Rh4AG116800 Rh4AG116900 Rh4AG117000 Rh4AG117100 Rh4BG109300 Rh4BG109400 Rh4BG109500 Rh4BG109600 Rh4BG109700 Rh4BG109800 Rh4CG096100 Rh4CG123700 Rh4CG124200
rosa_wichuraiana Rw4G007280 Rw4G009320 Rw4G009340 Rw4G009370 Rw4G009380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 541
AccI GTMKAC 1 cut(s) 71
AciI CCGC 2 cut(s) 375, 553
AclWI GGATC 1 cut(s) 313
AcuI CTGAAG 1 cut(s) 130
AfaI GTAC 3 cut(s) 336, 445, 484
AfiI CCNNNNNNNGG 1 cut(s) 363
AgsI TTSAA 4 cut(s) 58, 283, 316, 438
AhlI ACTAGT 1 cut(s) 440
AluBI AGCT 2 cut(s) 13, 475
AluI AGCT 2 cut(s) 13, 475
AlwI GGATC 1 cut(s) 313
Ama87I CYCGRG 1 cut(s) 340
AoxI GGCC 1 cut(s) 565
ApeKI GCWGC 2 cut(s) 4, 172
AspLEI GCGC 2 cut(s) 243, 542
AspS9I GGNCC 1 cut(s) 565
AvaI CYCGRG 1 cut(s) 340
AxyI CCTNAGG 1 cut(s) 561
BbsI GAAGAC 1 cut(s) 405
BbvI GCAGC 2 cut(s) 16, 184
BccI CCATC 1 cut(s) 254
BcuI ACTAGT 1 cut(s) 440
BfaI CTAG 1 cut(s) 441
BisI GCNGC 2 cut(s) 5, 173
BlsI GCNGC 2 cut(s) 6, 174
BmcAI AGTACT 1 cut(s) 445
BmeT110I CYCGRG 1 cut(s) 340
BmgT120I GGNCC 1 cut(s) 565
BmsI GCATC 1 cut(s) 431
BpiI GAAGAC 1 cut(s) 405
BpmI CTGGAG 1 cut(s) 438
BpuEI CTTGAG 1 cut(s) 130
BsaJI CCNNGG 1 cut(s) 151
Bsc4I CCNNNNNNNGG 1 cut(s) 363
Bse21I CCTNAGG 1 cut(s) 561
Bse3DI GCAATG 1 cut(s) 333
BseDI CCNNGG 1 cut(s) 151
BseGI GGATG 2 cut(s) 85, 504
BseLI CCNNNNNNNGG 1 cut(s) 363
BseMI GCAATG 1 cut(s) 333
BseMII CTCAG 1 cut(s) 575
BseXI GCAGC 2 cut(s) 16, 184
BsgI GTGCAG 1 cut(s) 530
BshFI GGCC 1 cut(s) 567
BsiHKCI CYCGRG 1 cut(s) 340
BsiSI CCGG 1 cut(s) 21
BslI CCNNNNNNNGG 1 cut(s) 363
BsmI GAATGC 1 cut(s) 584
BsnI GGCC 1 cut(s) 567
BsoBI CYCGRG 1 cut(s) 340
Bsp143I GATC 2 cut(s) 318, 525
BspACI CCGC 2 cut(s) 375, 553
BspANI GGCC 1 cut(s) 567
BspCNI CTCAG 1 cut(s) 574
BspPI GGATC 1 cut(s) 313
BsrDI GCAATG 1 cut(s) 333
BssECI CCNNGG 1 cut(s) 151
BssMI GATC 2 cut(s) 318, 525
BssT1I CCWWGG 1 cut(s) 151
Bst4CI ACNGT 2 cut(s) 40, 482
BstDEI CTNAG 2 cut(s) 561, 591
BstF5I GGATG 2 cut(s) 85, 504
BstHHI GCGC 2 cut(s) 243, 542
BstKTI GATC 2 cut(s) 321, 528
BstMBI GATC 2 cut(s) 318, 525
BstMWI GCNNNNNNNGC 1 cut(s) 10
BstV1I GCAGC 2 cut(s) 16, 184
BstV2I GAAGAC 1 cut(s) 405
BstX2I RGATCY 1 cut(s) 318
BstYI RGATCY 1 cut(s) 318
Bsu36I CCTNAGG 1 cut(s) 561
BsuRI GGCC 1 cut(s) 567
BtsCI GGATG 2 cut(s) 85, 504
CfoI GCGC 2 cut(s) 243, 542
Cfr13I GGNCC 1 cut(s) 565
Csp6I GTAC 3 cut(s) 335, 444, 483
CviAII CATG 3 cut(s) 212, 523, 572
CviJI RGCY 7 cut(s) 7, 13, 47, 150, 345, 475, 567
CviKI_1 RGCY 7 cut(s) 7, 13, 47, 150, 345, 475, 567
CviQI GTAC 3 cut(s) 335, 444, 483
DdeI CTNAG 2 cut(s) 561, 591
DpnI GATC 2 cut(s) 320, 527
DpnII GATC 2 cut(s) 318, 525
Eco130I CCWWGG 1 cut(s) 151
Eco57I CTGAAG 1 cut(s) 130
Eco81I CCTNAGG 1 cut(s) 561
Eco88I CYCGRG 1 cut(s) 340
EcoO109I RGGNCCY 1 cut(s) 565
EcoT14I CCWWGG 1 cut(s) 151
ErhI CCWWGG 1 cut(s) 151
FaeI CATG 3 cut(s) 215, 526, 575
FaiI YATR 3 cut(s) 213, 524, 573
FatI CATG 3 cut(s) 211, 522, 571
FblI GTMKAC 1 cut(s) 71
Fnu4HI GCNGC 2 cut(s) 5, 173
FokI GGATG 2 cut(s) 92, 511
Fsp4HI GCNGC 2 cut(s) 5, 173
FspBI CTAG 1 cut(s) 441
FspI TGCGCA 1 cut(s) 541
GlaI GCGC 2 cut(s) 242, 541
GluI GCNGC 2 cut(s) 5, 173
GsuI CTGGAG 1 cut(s) 438
HaeIII GGCC 1 cut(s) 567
HapII CCGG 1 cut(s) 21
HhaI GCGC 2 cut(s) 243, 542
Hin1II CATG 3 cut(s) 215, 526, 575
Hin6I GCGC 2 cut(s) 241, 540
HinP1I GCGC 2 cut(s) 241, 540
HindIII AAGCTT 1 cut(s) 11
HinfI GANTC 3 cut(s) 232, 286, 293
HpaII CCGG 1 cut(s) 21
Hpy166II GTNNAC 3 cut(s) 72, 337, 380
Hpy188I TCNGA 1 cut(s) 64
Hpy188III TCNNGA 7 cut(s) 229, 283, 290, 313, 349, 417, 491
Hpy8I GTNNAC 3 cut(s) 72, 337, 380
HpyAV CCTTC 1 cut(s) 59
HpyCH4III ACNGT 2 cut(s) 40, 482
HpyCH4V TGCA 5 cut(s) 4, 405, 432, 511, 582
HpyF10VI GCNNNNNNNGC 1 cut(s) 10
HpyF3I CTNAG 2 cut(s) 561, 591
Hsp92II CATG 3 cut(s) 215, 526, 575
HspAI GCGC 2 cut(s) 241, 540
Kzo9I GATC 2 cut(s) 318, 525
LmnI GCTCC 1 cut(s) 419
LpnPI CCDG 8 cut(s) 18, 34, 172, 214, 334, 402, 461, 548
Lsp1109I GCAGC 2 cut(s) 16, 184
LweI GCATC 1 cut(s) 431
MaeI CTAG 1 cut(s) 441
MaeIII GTNAC 1 cut(s) 40
MalI GATC 2 cut(s) 320, 527
MboI GATC 2 cut(s) 318, 525
MboII GAAGA 4 cut(s) 122, 136, 328, 405
MflI RGATCY 1 cut(s) 318
MluCI AATT 2 cut(s) 176, 532
MlyI GAGTC 1 cut(s) 302
MnlI CCTC 4 cut(s) 194, 356, 570, 578
MspI CCGG 1 cut(s) 21
Mva1269I GAATGC 1 cut(s) 584
MwoI GCNNNNNNNGC 1 cut(s) 10
NdeII GATC 2 cut(s) 318, 525
NlaIII CATG 3 cut(s) 215, 526, 575
NmuCI GTSAC 1 cut(s) 40
NsbI TGCGCA 1 cut(s) 541
PaeR7I CTCGAG 1 cut(s) 340
PctI GAATGC 1 cut(s) 584
PfeI GAWTC 2 cut(s) 232, 286
PkrI GCNGC 2 cut(s) 6, 174
PleI GAGTC 1 cut(s) 301
PpsI GAGTC 1 cut(s) 301
PspPI GGNCC 1 cut(s) 565
PspXI VCTCGAGB 1 cut(s) 340
PsuI RGATCY 1 cut(s) 318
RsaI GTAC 3 cut(s) 336, 445, 484
RsaNI GTAC 3 cut(s) 335, 444, 483
SatI GCNGC 2 cut(s) 5, 173
Sau3AI GATC 2 cut(s) 318, 525
Sau96I GGNCC 1 cut(s) 565
ScaI AGTACT 1 cut(s) 445
SchI GAGTC 1 cut(s) 302
SetI ASST 4 cut(s) 15, 186, 304, 477
SfaNI GCATC 1 cut(s) 431
Sfr274I CTCGAG 1 cut(s) 340
SlaI CTCGAG 1 cut(s) 340
SmlI CTYRAG 2 cut(s) 109, 340
SmoI CTYRAG 2 cut(s) 109, 340
SpeI ACTAGT 1 cut(s) 440
Sse9I AATT 2 cut(s) 176, 532
SsiI CCGC 2 cut(s) 375, 553
SspMI CTAG 1 cut(s) 441
StyI CCWWGG 1 cut(s) 151
TaaI ACNGT 2 cut(s) 40, 482
TaqI TCGA 2 cut(s) 296, 341
TasI AATT 2 cut(s) 176, 532
TatI WGTACW 3 cut(s) 334, 443, 482
TfiI GAWTC 2 cut(s) 232, 286
TseFI GTSAC 1 cut(s) 40
TseI GCWGC 2 cut(s) 4, 172
Tsp45I GTSAC 1 cut(s) 40
TspDTI ATGAA 2 cut(s) 211, 515
XhoI CTCGAG 1 cut(s) 340
XmiI GTMKAC 1 cut(s) 71
XspI CTAG 1 cut(s) 441
ZrmI AGTACT 1 cut(s) 445
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.