Rh4AG116500

Catalyzes xyloglucan endohydrolysis (XEH) and or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0000460
Physical Location & Seq
Forward (+)
25142899 .. 25143267
369 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG116500.1

Sequence Viewer

Length: 369 bp
ATGGAATCAATTGGCGTTCCATTCCCAAAGAACCAGCCAATGAGATTAACCTCTAGTCTCTGGAATGCTGATGATTGGGCAACAAGGGGAGGGCTTGTGAAGACAGACTGGAGCAAAGCTCCTTTCACTGCTTCCTACAGGAACTTCAATGCCGACAAGGCTTGTATTTGGTCAAGGACTAAAAAAACATCTTCGTGCAGCTCGTCATCGTCATCGTCTAAAATACCTGGTAATGAATGGCTCACAGAAGATCTCGACACAACGAAGCAGGGGAGGCTGAGATGGGTGCAGAAGAACTACATGATCTATAATTACTGCTCTGACATTAAGAGATTTCCCCAGGGACTCCCTACTGAATGCAAGTCGTAA

Protein Analysis

122

Amino Acids

13.91

Weight (kDa)

9.42

Isoelectric Point (pI)

60.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_16 PF00722 5 - 39 3e-08 Glycosyl hydrolases family 16
XET_C PF06955 78 - 120 4e-21 Xyloglucan endo-transglycosylase (XET) C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000460)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G25810 AT4G30270 AT5G57550 AT5G57560
fragaria_vesca FvH4_3g00833 FvH4_3g00840 FvH4_3g00850 FvH4_4g06700 FvH4_4g09230
malus_domestica MD13G1237300.v1.1 MD13G1268000.v1.1 MD13G1268900.v1.1 MD16G1267200.v1.1 MD16G1267300.v1.1
prunus_persica Prupe.1G069800_v2.0.a1 Prupe.1G088600_v2.0.a1 Prupe.1G088800_v2.0.a1 Prupe.1G088900_v2.0.a1 Prupe.1G089000_v2.0.a1
pyrus_communis pycom13g21020 pycom13g22350 pycom13g22380 pycom16g23630 pycom16g23640
rosa_chinensis RchiOBHm_Chr4g0398631 RchiOBHm_Chr4g0402891 RchiOBHm_Chr4g0402911 RchiOBHm_Chr4g0402921 RchiOBHm_Chr4g0402931 RchiOBHm_Chr4g0402951 RchiOBHm_Chr4g0402961 RchiOBHm_Chr4g0402981
rosa_laevigata RLG00000009031 RLG00000009033 RLG00000009034 RLG00000009035 RLG00000009039 RLG00000009040 RLG00000009339
rosa_multiflora Rmu_co8186292.1_g000001 Rmu_sc0000990.1_g000004 Rmu_sc0001023.1_g000002 Rmu_sc0001023.1_g000004 Rmu_sc0001023.1_g000007 Rmu_sc0001023.1_g000015 Rmu_sc0002178.1_g000007 Rmu_sc0002451.1_g000069 Rmu_sc0002451.1_g000079 Rmu_sc0002451.1_g000080 Rmu_sc0008058.1_g000026 Rmu_sc0008058.1_g000029 Rmu_sc0008058.1_g000030
rosa_roxburghii Rroxscaffold_5G00343590 Rroxscaffold_5G00347620 Rroxscaffold_5G00347630 Rroxscaffold_5G00347640 Rroxscaffold_5G00347650 Rroxscaffold_5G00347660 Rroxscaffold_5G00347670 Rroxscaffold_5G00347690
rosa_rugosa Rorug04G0009900 Rorug04G0039000 Rorug04G0039100 Rorug04G0039200 Rorug04G0039300 Rorug04G0039400 Rorug04G0039500 Rorug04G0039600 Rorug04G0039600 Rorug04G0040200
rosa_samantha Rh4AG087800 Rh4AG116500 Rh4AG116700 Rh4AG116800 Rh4AG116900 Rh4AG117000 Rh4AG117100 Rh4BG109300 Rh4BG109400 Rh4BG109500 Rh4BG109600 Rh4BG109700 Rh4BG109800 Rh4CG096100 Rh4CG123700 Rh4CG124200
rosa_wichuraiana Rw4G007280 Rw4G009320 Rw4G009340 Rw4G009370 Rw4G009380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AgsI TTSAA 1 cut(s) 148
AjnI CCWGG 2 cut(s) 226, 339
AluBI AGCT 2 cut(s) 119, 201
AluI AGCT 2 cut(s) 119, 201
Alw26I GTCTC 1 cut(s) 62
ApeKI GCWGC 1 cut(s) 198
BbsI GAAGAC 1 cut(s) 107
BbvI GCAGC 1 cut(s) 210
BccI CCATC 1 cut(s) 276
BciT130I CCWGG 2 cut(s) 228, 341
BcoDI GTCTC 1 cut(s) 62
BfaI CTAG 1 cut(s) 54
BfmI CTRYAG 1 cut(s) 136
BglI GCCNNNNNGGC 1 cut(s) 158
BglII AGATCT 1 cut(s) 250
BisI GCNGC 1 cut(s) 199
BlsI GCNGC 1 cut(s) 200
Bme1390I CCNGG 2 cut(s) 228, 341
BmrFI CCNGG 2 cut(s) 228, 341
BpiI GAAGAC 1 cut(s) 107
BplI GAGNNNNNCTC 2 cut(s) 103, 135
BpmI CTGGAG 1 cut(s) 130
BsaJI CCNNGG 2 cut(s) 339, 340
BsaXI ACNNNNNCTCC 2 cut(s) 81, 111
Bse1I ACTGG 1 cut(s) 113
BseBI CCWGG 2 cut(s) 228, 341
BseDI CCNNGG 2 cut(s) 339, 340
BseMII CTCAG 1 cut(s) 269
BseNI ACTGG 1 cut(s) 113
BseXI GCAGC 1 cut(s) 210
BsgI GTGCAG 2 cut(s) 217, 308
BslFI GGGAC 1 cut(s) 357
BsmAI GTCTC 1 cut(s) 62
BsmFI GGGAC 1 cut(s) 357
BsmI GAATGC 2 cut(s) 70, 362
Bsp143I GATC 2 cut(s) 250, 303
BspCNI CTCAG 1 cut(s) 270
BsrI ACTGG 1 cut(s) 113
BssECI CCNNGG 2 cut(s) 339, 340
BssMI GATC 2 cut(s) 250, 303
Bst2UI CCWGG 2 cut(s) 228, 341
BstDEI CTNAG 1 cut(s) 278
BstKTI GATC 2 cut(s) 253, 306
BstMAI GTCTC 1 cut(s) 62
BstMBI GATC 2 cut(s) 250, 303
BstMWI GCNNNNNNNGC 2 cut(s) 158, 274
BstNI CCWGG 2 cut(s) 228, 341
BstSCI CCNGG 2 cut(s) 226, 339
BstSFI CTRYAG 1 cut(s) 136
BstV1I GCAGC 1 cut(s) 210
BstV2I GAAGAC 1 cut(s) 107
BstX2I RGATCY 1 cut(s) 250
BstYI RGATCY 1 cut(s) 250
BtsI GCAGTG 1 cut(s) 126
BtsIMutI CAGTG 1 cut(s) 126
CsiI ACCWGGT 1 cut(s) 226
CviAII CATG 1 cut(s) 301
CviJI RGCY 7 cut(s) 37, 94, 119, 161, 201, 241, 277
CviKI_1 RGCY 7 cut(s) 37, 94, 119, 161, 201, 241, 277
DdeI CTNAG 1 cut(s) 278
DpnI GATC 2 cut(s) 252, 305
DpnII GATC 2 cut(s) 250, 303
EcoRII CCWGG 2 cut(s) 226, 339
FaeI CATG 1 cut(s) 304
FaiI YATR 2 cut(s) 302, 309
FaqI GGGAC 1 cut(s) 357
FatI CATG 1 cut(s) 300
Fnu4HI GCNGC 1 cut(s) 199
Fsp4HI GCNGC 1 cut(s) 199
FspBI CTAG 1 cut(s) 54
GluI GCNGC 1 cut(s) 199
GsuI CTGGAG 1 cut(s) 130
Hin1II CATG 1 cut(s) 304
HinfI GANTC 2 cut(s) 5, 345
Hpy188I TCNGA 1 cut(s) 322
Hpy188III TCNNGA 2 cut(s) 61, 254
HpyCH4V TGCA 3 cut(s) 198, 289, 360
HpyF10VI GCNNNNNNNGC 2 cut(s) 158, 274
HpyF3I CTNAG 1 cut(s) 278
Hsp92II CATG 1 cut(s) 304
Kzo9I GATC 2 cut(s) 250, 303
LmnI GCTCC 2 cut(s) 111, 124
LpnPI CCDG 9 cut(s) 46, 47, 94, 124, 213, 240, 254, 326, 353
Lsp1109I GCAGC 1 cut(s) 210
MabI ACCWGGT 1 cut(s) 226
MaeI CTAG 1 cut(s) 54
MalI GATC 2 cut(s) 252, 305
MboI GATC 2 cut(s) 250, 303
MboII GAAGA 4 cut(s) 112, 183, 260, 304
MfeI CAATTG 1 cut(s) 9
MflI RGATCY 1 cut(s) 250
MluCI AATT 2 cut(s) 9, 310
MlyI GAGTC 1 cut(s) 339
MnlI CCTC 3 cut(s) 61, 83, 267
MseI TTAA 2 cut(s) 47, 327
MslI CAYNNNNRTG 1 cut(s) 193
MspR9I CCNGG 2 cut(s) 228, 341
MunI CAATTG 1 cut(s) 9
Mva1269I GAATGC 2 cut(s) 70, 362
MvaI CCWGG 2 cut(s) 228, 341
MwoI GCNNNNNNNGC 2 cut(s) 158, 274
NdeII GATC 2 cut(s) 250, 303
NlaIII CATG 1 cut(s) 304
PasI CCCWGGG 1 cut(s) 340
PcsI WCGNNNNNNNCGW 1 cut(s) 200
PctI GAATGC 2 cut(s) 70, 362
PfeI GAWTC 1 cut(s) 5
PkrI GCNGC 1 cut(s) 200
PleI GAGTC 1 cut(s) 339
PpsI GAGTC 1 cut(s) 339
Psp6I CCWGG 2 cut(s) 226, 339
PspGI CCWGG 2 cut(s) 226, 339
PsuI RGATCY 1 cut(s) 250
RseI CAYNNNNRTG 1 cut(s) 193
SaqAI TTAA 2 cut(s) 47, 327
SatI GCNGC 1 cut(s) 199
Sau3AI GATC 2 cut(s) 250, 303
SchI GAGTC 1 cut(s) 339
ScrFI CCNGG 2 cut(s) 228, 341
SetI ASST 4 cut(s) 53, 121, 203, 229
SexAI ACCWGGT 1 cut(s) 226
SfcI CTRYAG 1 cut(s) 136
SmiMI CAYNNNNRTG 1 cut(s) 193
Sse9I AATT 2 cut(s) 9, 310
SspMI CTAG 1 cut(s) 54
StyD4I CCNGG 2 cut(s) 226, 339
TaqI TCGA 1 cut(s) 255
TasI AATT 2 cut(s) 9, 310
TfiI GAWTC 1 cut(s) 5
Tru1I TTAA 2 cut(s) 47, 327
Tru9I TTAA 2 cut(s) 47, 327
TscAI CASTG 1 cut(s) 133
TseI GCWGC 1 cut(s) 198
TspDTI ATGAA 1 cut(s) 249
TspRI CASTG 1 cut(s) 133
XspI CTAG 1 cut(s) 54
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.