FvH4_4g06700

Xyloglucan endo-transglycosylase (XET) C-terminus

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0000460
Physical Location & Seq
Reverse (-)
6003308 .. 6005369
2062 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g06700.t1

Sequence Viewer

Length: 858 bp
ATGGCTAAGATATATGCTCTAGCTTTGGTGATGTTCTTCAAAATCTTGGTGGCCGCATCGGCTGGTAACTTCAACCAAGATTTTGATATAACATGGGGCGACGGGCGGGCCAAAATACTCAATAACGCGCAGCTACTCACGTTAGCTCTTGACAAGACTTCCGGGTCGGGATTCAAGTCCAGGAATCAGTACCTGTTTGGAAAAATTGATATGCAGATTAAGCTCGTGCCCGGTAACTCCGCTGGTACCGTTACCTCTTACTATCTATCTTCGTTGGGGTCGGCTCATGATGAAATAGACTTTGAGTTTCTTGGCAACCTAAGCGGAGATCCTTATACTCTGCACACAAATGTTTTCACACAAGGCAAGGGTAACAGAGAGCAGCAATTCTATCTTTGGTTCGATCCTACAAAGGACTTCCACACCTACTCCATCTTGTGGAACCCTCAAAGCATCATATTCTCTGTGGACGGGACACCTATTCGAGAGTTCAAGAACTTAGAATCGAGGGGAATCCCATTCCCAAAGAACCAAGCAATGTGGATCTACTCGAGCCTTTGGAACGCAGACGATTGGGCAACACGCGGTGGACTTGTGAAGACAGACTGGAGTAAAGCCCCATTCACAGCCTCGTACAGAAACTTCAACGCCCAGGCATGCATTTGGTCTTCTGGTTCTTCCTCGTGTTCTTCTTCTCCTTCCGGTTCTTCAAAAGAGGCATGGTTCACCCAGTCACTGGATGCAACAGGGAAAGGAAGAATGAAATGGGTTCAGAAGAACTATATGATCTACGACTATTGCAAGGATACCAAGCGTTTCCCGCAGGGAGTTCCTCTTGAATGCACAGTTGCTACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
Glyco_hydro_16 XET_C

Protein Analysis

286

Amino Acids

31.97

Weight (kDa)

8.74

Isoelectric Point (pI)

32.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_16 PF00722 24 - 205 5.5e-67 Glycosyl hydrolases family 16
XET_C PF06955 237 - 281 1.9e-22 Xyloglucan endo-transglycosylase (XET) C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000460)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G25810 AT4G30270 AT5G57550 AT5G57560
fragaria_vesca FvH4_3g00833 FvH4_3g00840 FvH4_3g00850 FvH4_4g06700 FvH4_4g09230
malus_domestica MD13G1237300.v1.1 MD13G1268000.v1.1 MD13G1268900.v1.1 MD16G1267200.v1.1 MD16G1267300.v1.1
prunus_persica Prupe.1G069800_v2.0.a1 Prupe.1G088600_v2.0.a1 Prupe.1G088800_v2.0.a1 Prupe.1G088900_v2.0.a1 Prupe.1G089000_v2.0.a1
pyrus_communis pycom13g21020 pycom13g22350 pycom13g22380 pycom16g23630 pycom16g23640
rosa_chinensis RchiOBHm_Chr4g0398631 RchiOBHm_Chr4g0402891 RchiOBHm_Chr4g0402911 RchiOBHm_Chr4g0402921 RchiOBHm_Chr4g0402931 RchiOBHm_Chr4g0402951 RchiOBHm_Chr4g0402961 RchiOBHm_Chr4g0402981
rosa_laevigata RLG00000009031 RLG00000009033 RLG00000009034 RLG00000009035 RLG00000009039 RLG00000009040 RLG00000009339
rosa_multiflora Rmu_co8186292.1_g000001 Rmu_sc0000990.1_g000004 Rmu_sc0001023.1_g000002 Rmu_sc0001023.1_g000004 Rmu_sc0001023.1_g000007 Rmu_sc0001023.1_g000015 Rmu_sc0002178.1_g000007 Rmu_sc0002451.1_g000069 Rmu_sc0002451.1_g000079 Rmu_sc0002451.1_g000080 Rmu_sc0008058.1_g000026 Rmu_sc0008058.1_g000029 Rmu_sc0008058.1_g000030
rosa_roxburghii Rroxscaffold_5G00343590 Rroxscaffold_5G00347620 Rroxscaffold_5G00347630 Rroxscaffold_5G00347640 Rroxscaffold_5G00347650 Rroxscaffold_5G00347660 Rroxscaffold_5G00347670 Rroxscaffold_5G00347690
rosa_rugosa Rorug04G0009900 Rorug04G0039000 Rorug04G0039100 Rorug04G0039200 Rorug04G0039300 Rorug04G0039400 Rorug04G0039500 Rorug04G0039600 Rorug04G0039600 Rorug04G0040200
rosa_samantha Rh4AG087800 Rh4AG116500 Rh4AG116700 Rh4AG116800 Rh4AG116900 Rh4AG117000 Rh4AG117100 Rh4BG109300 Rh4BG109400 Rh4BG109500 Rh4BG109600 Rh4BG109700 Rh4BG109800 Rh4CG096100 Rh4CG123700 Rh4CG124200
rosa_wichuraiana Rw4G007280 Rw4G009320 Rw4G009340 Rw4G009370 Rw4G009380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 163
Acc65I GGTACC 1 cut(s) 245
AccB1I GGYRCC 1 cut(s) 245
AccB7I CCANNNNNTGG 2 cut(s) 438, 736
AccII CGCG 2 cut(s) 128, 585
AciI CCGC 6 cut(s) 54, 106, 240, 324, 585, 821
AclWI GGATC 3 cut(s) 323, 398, 551
AcoI YGGCCR 1 cut(s) 51
AdeI CACNNNGTG 1 cut(s) 587
AfaI GTAC 3 cut(s) 191, 247, 635
AfiI CCNNNNNNNGG 2 cut(s) 438, 736
AgsI TTSAA 7 cut(s) 40, 73, 175, 493, 646, 711, 839
AjnI CCWGG 2 cut(s) 179, 651
AluBI AGCT 4 cut(s) 23, 133, 146, 223
AluI AGCT 4 cut(s) 23, 133, 146, 223
AlwI GGATC 3 cut(s) 323, 398, 551
AlwNI CAGNNNCTG 2 cut(s) 193, 736
Ama87I CYCGRG 1 cut(s) 550
AoxI GGCC 2 cut(s) 51, 108
ApeKI GCWGC 2 cut(s) 130, 382
Asp718I GGTACC 1 cut(s) 245
AspLEI GCGC 1 cut(s) 130
AspS9I GGNCC 1 cut(s) 108
AsuC2I CCSGG 2 cut(s) 163, 231
AsuHPI GGTGA 2 cut(s) 40, 718
AvaI CYCGRG 1 cut(s) 550
BaeGI GKGCMC 1 cut(s) 231
BanI GGYRCC 1 cut(s) 245
BarI GAAGNNNNNNTAC 2 cut(s) 626, 658
BauI CACGAG 2 cut(s) 224, 682
BbsI GAAGAC 2 cut(s) 605, 660
BbvI GCAGC 2 cut(s) 142, 394
BccI CCATC 1 cut(s) 440
BciT130I CCWGG 2 cut(s) 181, 653
BciVI GTATCC 1 cut(s) 799
BcnI CCSGG 2 cut(s) 163, 231
BfaI CTAG 1 cut(s) 20
BfuI GTATCC 1 cut(s) 799
BglI GCCNNNNNGGC 1 cut(s) 59
BisI GCNGC 3 cut(s) 54, 131, 383
BlsI GCNGC 3 cut(s) 55, 132, 384
Bme1390I CCNGG 4 cut(s) 163, 181, 231, 653
BmeT110I CYCGRG 1 cut(s) 550
BmgT120I GGNCC 1 cut(s) 108
BmiI GGNNCC 2 cut(s) 247, 443
BmrFI CCNGG 4 cut(s) 163, 181, 231, 653
BmrI ACTGGG 1 cut(s) 724
BmsI GCATC 3 cut(s) 65, 462, 730
BmuI ACTGGG 1 cut(s) 724
BpiI GAAGAC 2 cut(s) 605, 660
BpmI CTGGAG 1 cut(s) 628
Bpu10I CCTNAGC 1 cut(s) 320
BpuMI CCSGG 2 cut(s) 163, 231
BsaJI CCNNGG 1 cut(s) 651
BsaWI WCCGGW 1 cut(s) 701
BsaXI ACNNNNNCTCC 2 cut(s) 413, 443
Bsc4I CCNNNNNNNGG 2 cut(s) 438, 736
Bse1I ACTGG 3 cut(s) 611, 730, 741
Bse3DI GCAATG 1 cut(s) 543
BseBI CCWGG 2 cut(s) 181, 653
BseDI CCNNGG 1 cut(s) 651
BseGI GGATG 1 cut(s) 745
BseLI CCNNNNNNNGG 2 cut(s) 438, 736
BseMI GCAATG 1 cut(s) 543
BseNI ACTGG 3 cut(s) 611, 730, 741
BseSI GKGCMC 1 cut(s) 231
BseXI GCAGC 2 cut(s) 142, 394
BsgI GTGCAG 1 cut(s) 326
Bsh1236I CGCG 2 cut(s) 128, 585
BshFI GGCC 2 cut(s) 53, 110
BshNI GGYRCC 1 cut(s) 245
BsiHKCI CYCGRG 1 cut(s) 550
BsiSI CCGG 3 cut(s) 162, 231, 702
BslFI GGGAC 1 cut(s) 487
BslI CCNNNNNNNGG 2 cut(s) 438, 736
BsmFI GGGAC 1 cut(s) 487
BsmI GAATGC 1 cut(s) 845
BsnI GGCC 2 cut(s) 53, 110
BsoBI CYCGRG 1 cut(s) 550
Bsp1286I GDGCHC 1 cut(s) 231
Bsp143I GATC 4 cut(s) 328, 403, 543, 786
BspACI CCGC 6 cut(s) 54, 106, 240, 324, 585, 821
BspANI GGCC 2 cut(s) 53, 110
BspFNI CGCG 2 cut(s) 128, 585
BspHI TCATGA 1 cut(s) 286
BspLI GGNNCC 2 cut(s) 247, 443
BspPI GGATC 3 cut(s) 323, 398, 551
BspT107I GGYRCC 1 cut(s) 245
BsrDI GCAATG 1 cut(s) 543
BsrI ACTGG 3 cut(s) 611, 730, 741
BssECI CCNNGG 1 cut(s) 651
BssMI GATC 4 cut(s) 328, 403, 543, 786
BssSI CACGAG 2 cut(s) 224, 682
Bst2BI CACGAG 2 cut(s) 224, 682
Bst2UI CCWGG 2 cut(s) 181, 653
Bst4CI ACNGT 2 cut(s) 250, 847
BstC8I GCNNGC 2 cut(s) 108, 658
BstDEI CTNAG 3 cut(s) 6, 320, 499
BstF5I GGATG 1 cut(s) 745
BstFNI CGCG 2 cut(s) 128, 585
BstHHI GCGC 1 cut(s) 130
BstKTI GATC 4 cut(s) 331, 406, 546, 789
BstMBI GATC 4 cut(s) 328, 403, 543, 786
BstMWI GCNNNNNNNGC 4 cut(s) 59, 220, 321, 820
BstNI CCWGG 2 cut(s) 181, 653
BstNSI RCATGY 1 cut(s) 660
BstSCI CCNGG 4 cut(s) 161, 179, 229, 651
BstSLI GKGCMC 1 cut(s) 231
BstUI CGCG 2 cut(s) 128, 585
BstV1I GCAGC 2 cut(s) 142, 394
BstV2I GAAGAC 2 cut(s) 605, 660
BstX2I RGATCY 2 cut(s) 328, 543
BstYI RGATCY 2 cut(s) 328, 543
BsuI GTATCC 1 cut(s) 799
BsuRI GGCC 2 cut(s) 53, 110
BtsCI GGATG 1 cut(s) 745
BtsIMutI CAGTG 1 cut(s) 734
Cac8I GCNNGC 2 cut(s) 108, 658
CaiI CAGNNNCTG 2 cut(s) 193, 736
CciI TCATGA 1 cut(s) 286
CfoI GCGC 1 cut(s) 130
Cfr13I GGNCC 1 cut(s) 108
Csp6I GTAC 3 cut(s) 190, 246, 634
CspCI CAANNNNNGTGG 2 cut(s) 521, 556
CviAII CATG 4 cut(s) 93, 287, 657, 720
CviQI GTAC 3 cut(s) 190, 246, 634
DdeI CTNAG 3 cut(s) 6, 320, 499
DpnI GATC 4 cut(s) 330, 405, 545, 788
DpnII GATC 4 cut(s) 328, 403, 543, 786
DraIII CACNNNGTG 1 cut(s) 587
DrdI GACNNNNNNGTC 1 cut(s) 163
DseDI GACNNNNNNGTC 1 cut(s) 163
EaeI YGGCCR 1 cut(s) 51
Eco88I CYCGRG 1 cut(s) 550
EcoRII CCWGG 2 cut(s) 179, 651
EcoT22I ATGCAT 1 cut(s) 662
FaeI CATG 4 cut(s) 96, 290, 660, 723
FaqI GGGAC 1 cut(s) 487
FatI CATG 4 cut(s) 92, 286, 656, 719
FauI CCCGC 2 cut(s) 99, 828
Fnu4HI GCNGC 3 cut(s) 54, 131, 383
FokI GGATG 1 cut(s) 752
Fsp4HI GCNGC 3 cut(s) 54, 131, 383
FspBI CTAG 1 cut(s) 20
GlaI GCGC 1 cut(s) 129
GluI GCNGC 3 cut(s) 54, 131, 383
GsuI CTGGAG 1 cut(s) 628
HaeIII GGCC 2 cut(s) 53, 110
HapII CCGG 3 cut(s) 162, 231, 702
HhaI GCGC 1 cut(s) 130
Hin1II CATG 4 cut(s) 96, 290, 660, 723
Hin6I GCGC 1 cut(s) 128
HinP1I GCGC 1 cut(s) 128
HinfI GANTC 4 cut(s) 171, 184, 503, 513
HpaII CCGG 3 cut(s) 162, 231, 702
HphI GGTGA 2 cut(s) 40, 718
Hpy166II GTNNAC 3 cut(s) 469, 590, 726
Hpy188I TCNGA 1 cut(s) 774
Hpy188III TCNNGA 6 cut(s) 149, 168, 287, 485, 493, 836
Hpy8I GTNNAC 3 cut(s) 469, 590, 726
Hpy99I CGWCG 1 cut(s) 104
HpyAV CCTTC 1 cut(s) 708
HpyCH4III ACNGT 2 cut(s) 250, 847
HpyCH4IV ACGT 1 cut(s) 140
HpyCH4V TGCA 6 cut(s) 214, 343, 660, 743, 801, 843
HpyF10VI GCNNNNNNNGC 4 cut(s) 59, 220, 321, 820
HpyF3I CTNAG 3 cut(s) 6, 320, 499
HpySE526I ACGT 1 cut(s) 140
Hsp92II CATG 4 cut(s) 96, 290, 660, 723
HspAI GCGC 1 cut(s) 128
KpnI GGTACC 1 cut(s) 249
Kzo9I GATC 4 cut(s) 328, 403, 543, 786
Lsp1109I GCAGC 2 cut(s) 142, 394
LweI GCATC 3 cut(s) 65, 462, 730
MaeI CTAG 1 cut(s) 20
MaeII ACGT 1 cut(s) 140
MaeIII GTNAC 5 cut(s) 65, 233, 250, 371, 732
MalI GATC 4 cut(s) 330, 405, 545, 788
MboI GATC 4 cut(s) 328, 403, 543, 786
MflI RGATCY 2 cut(s) 328, 543
MhlI GDGCHC 1 cut(s) 231
MluCI AATT 2 cut(s) 204, 386
MnlI CCTC 7 cut(s) 265, 456, 501, 640, 691, 709, 843
Mph1103I ATGCAT 1 cut(s) 662
MseI TTAA 1 cut(s) 219
MslI CAYNNNNRTG 1 cut(s) 348
MspA1I CMGCKG 1 cut(s) 242
MspI CCGG 3 cut(s) 162, 231, 702
MspR9I CCNGG 4 cut(s) 163, 181, 231, 653
Mva1269I GAATGC 1 cut(s) 845
MvaI CCWGG 2 cut(s) 181, 653
MvnI CGCG 2 cut(s) 128, 585
MwoI GCNNNNNNNGC 4 cut(s) 59, 220, 321, 820
NciI CCSGG 2 cut(s) 163, 231
NdeII GATC 4 cut(s) 328, 403, 543, 786
NlaIII CATG 4 cut(s) 96, 290, 660, 723
NlaIV GGNNCC 2 cut(s) 247, 443
NmuCI GTSAC 1 cut(s) 732
NsiI ATGCAT 1 cut(s) 662
NspI RCATGY 1 cut(s) 660
PaeI GCATGC 1 cut(s) 660
PaeR7I CTCGAG 1 cut(s) 550
PagI TCATGA 1 cut(s) 286
PctI GAATGC 1 cut(s) 845
PfeI GAWTC 4 cut(s) 171, 184, 503, 513
PflMI CCANNNNNTGG 2 cut(s) 438, 736
PfoI TCCNGGA 1 cut(s) 179
PkrI GCNGC 3 cut(s) 55, 132, 384
Psp6I CCWGG 2 cut(s) 179, 651
PspGI CCWGG 2 cut(s) 179, 651
PspN4I GGNNCC 2 cut(s) 247, 443
PspPI GGNCC 1 cut(s) 108
PspXI VCTCGAGB 1 cut(s) 550
PstNI CAGNNNCTG 2 cut(s) 193, 736
PsuI RGATCY 2 cut(s) 328, 543
RsaI GTAC 3 cut(s) 191, 247, 635
RsaNI GTAC 3 cut(s) 190, 246, 634
RseI CAYNNNNRTG 1 cut(s) 348
SaqAI TTAA 1 cut(s) 219
SatI GCNGC 3 cut(s) 54, 131, 383
Sau3AI GATC 4 cut(s) 328, 403, 543, 786
Sau96I GGNCC 1 cut(s) 108
ScrFI CCNGG 4 cut(s) 163, 181, 231, 653
SduI GDGCHC 1 cut(s) 231
SfaNI GCATC 3 cut(s) 65, 462, 730
Sfr274I CTCGAG 1 cut(s) 550
SlaI CTCGAG 1 cut(s) 550
SmiMI CAYNNNNRTG 1 cut(s) 348
SmlI CTYRAG 1 cut(s) 550
SmoI CTYRAG 1 cut(s) 550
SphI GCATGC 1 cut(s) 660
Sse9I AATT 2 cut(s) 204, 386
SsiI CCGC 6 cut(s) 54, 106, 240, 324, 585, 821
SspMI CTAG 1 cut(s) 20
StyD4I CCNGG 4 cut(s) 161, 179, 229, 651
TaaI ACNGT 2 cut(s) 250, 847
TaiI ACGT 1 cut(s) 143
TaqI TCGA 4 cut(s) 402, 484, 506, 551
TasI AATT 2 cut(s) 204, 386
TauI GCSGC 1 cut(s) 56
TfiI GAWTC 4 cut(s) 171, 184, 503, 513
Tru1I TTAA 1 cut(s) 219
Tru9I TTAA 1 cut(s) 219
TscAI CASTG 1 cut(s) 741
TseFI GTSAC 1 cut(s) 732
TseI GCWGC 2 cut(s) 130, 382
Tsp45I GTSAC 1 cut(s) 732
TspDTI ATGAA 2 cut(s) 306, 776
TspRI CASTG 1 cut(s) 741
Van91I CCANNNNNTGG 2 cut(s) 438, 736
XceI RCATGY 1 cut(s) 660
XhoI CTCGAG 1 cut(s) 550
XspI CTAG 1 cut(s) 20
Zsp2I ATGCAT 1 cut(s) 662
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.