MD13G1268900.v1.1

Catalyzes xyloglucan endohydrolysis (XEH) and or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0000460
Physical Location & Seq
Reverse (-)
35412640 .. 35414637
1998 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1268900.v1.1.491

Sequence Viewer

Length: 834 bp
ATGGTTACCTCTTTGATGGTTGCTTCTGCTGGAAACTTCTACCAGGATATTCAGGTTACATGGGGTGATGGCCGCGGTAAGATACTAAACAACAGCGAGCTTCTTACTCTCTCACTTGACAAGTCCTCTGGTTCTGGCTTCCAATCCACAAATCAATATCTTTTTGGAAAAATTGATATGCAAATTAAGCTTGTTCCTGGTAACTCTGCAGGCACTGTCACTACCTATTACTTGCGCTCAGAAGGGTCTTCGTGGGATGAGATAGACTTTGAATTCTTGGGGAATCTTAGTGGCGACCCTTATATTGTTCATACCAATATATTCAGCCAAGGCAAAGGCAACAAAGAGCAGCAATTCTACCTCTGGTTCGACCCAACTGCTGATTTTCACACCTACTCTATCCTTTGGAATGCCCAACATATTGTCCTCTATGTTGATGGCACACCCATTAGAGAGTTCAAGAACTTGGAGTCAATTGGTGTTTCATACCCAAAGAACCAGTCCATGAGGATATATTCAAGCCTCTGGAACGCTGATGACTGGGCCACTAGAGGAGGTCTGGTCAAGGCAGATTGGTCCCAAGCTCCCTTTACTCCTTCGTACAGGAATTTCAATGCTGATAATGCTTGCATTTGGTGGTCTTCGGGTTCTACTTCTTGCAGTTCAAATTCAAACTCAACGAGCTCTGTCTTGCTCTCACAAGAATTGGACTCCACAAGCCAAGAGAGGCTTCAATGGGTGCAGAAGAATTACATGGTTTATAATTACTGCGCAGACACAAAGCGATTCCCGCAAGGCCTCCCTCTAGAATGCACTGCCAGCAACACATCTTAA

Protein Analysis

278

Amino Acids

31.13

Weight (kDa)

4.95

Isoelectric Point (pI)

38.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_16 PF00722 14 - 193 3.7e-64 Glycosyl hydrolases family 16
XET_C PF06955 229 - 271 1.5e-20 Xyloglucan endo-transglycosylase (XET) C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000460)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G25810 AT4G30270 AT5G57550 AT5G57560
fragaria_vesca FvH4_3g00833 FvH4_3g00840 FvH4_3g00850 FvH4_4g06700 FvH4_4g09230
malus_domestica MD13G1237300.v1.1 MD13G1268000.v1.1 MD13G1268900.v1.1 MD16G1267200.v1.1 MD16G1267300.v1.1
prunus_persica Prupe.1G069800_v2.0.a1 Prupe.1G088600_v2.0.a1 Prupe.1G088800_v2.0.a1 Prupe.1G088900_v2.0.a1 Prupe.1G089000_v2.0.a1
pyrus_communis pycom13g21020 pycom13g22350 pycom13g22380 pycom16g23630 pycom16g23640
rosa_chinensis RchiOBHm_Chr4g0398631 RchiOBHm_Chr4g0402891 RchiOBHm_Chr4g0402911 RchiOBHm_Chr4g0402921 RchiOBHm_Chr4g0402931 RchiOBHm_Chr4g0402951 RchiOBHm_Chr4g0402961 RchiOBHm_Chr4g0402981
rosa_laevigata RLG00000009031 RLG00000009033 RLG00000009034 RLG00000009035 RLG00000009039 RLG00000009040 RLG00000009339
rosa_multiflora Rmu_co8186292.1_g000001 Rmu_sc0000990.1_g000004 Rmu_sc0001023.1_g000002 Rmu_sc0001023.1_g000004 Rmu_sc0001023.1_g000007 Rmu_sc0001023.1_g000015 Rmu_sc0002178.1_g000007 Rmu_sc0002451.1_g000069 Rmu_sc0002451.1_g000079 Rmu_sc0002451.1_g000080 Rmu_sc0008058.1_g000026 Rmu_sc0008058.1_g000029 Rmu_sc0008058.1_g000030
rosa_roxburghii Rroxscaffold_5G00343590 Rroxscaffold_5G00347620 Rroxscaffold_5G00347630 Rroxscaffold_5G00347640 Rroxscaffold_5G00347650 Rroxscaffold_5G00347660 Rroxscaffold_5G00347670 Rroxscaffold_5G00347690
rosa_rugosa Rorug04G0009900 Rorug04G0039000 Rorug04G0039100 Rorug04G0039200 Rorug04G0039300 Rorug04G0039400 Rorug04G0039500 Rorug04G0039600 Rorug04G0039600 Rorug04G0040200
rosa_samantha Rh4AG087800 Rh4AG116500 Rh4AG116700 Rh4AG116800 Rh4AG116900 Rh4AG117000 Rh4AG117100 Rh4BG109300 Rh4BG109400 Rh4BG109500 Rh4BG109600 Rh4BG109700 Rh4BG109800 Rh4CG096100 Rh4CG123700 Rh4CG124200
rosa_wichuraiana Rw4G007280 Rw4G009320 Rw4G009340 Rw4G009370 Rw4G009380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 762
Acc16I TGCGCA 1 cut(s) 772
AccII CGCG 1 cut(s) 75
AciI CCGC 3 cut(s) 73, 75, 791
AcoI YGGCCR 1 cut(s) 70
AcsI RAATTY 3 cut(s) 272, 607, 667
AfaI GTAC 1 cut(s) 602
AgsI TTSAA 7 cut(s) 272, 460, 519, 613, 666, 672, 734
AjnI CCWGG 2 cut(s) 42, 196
AjuI GAANNNNNNNTTGG 2 cut(s) 714, 746
AluBI AGCT 4 cut(s) 100, 190, 584, 684
AluI AGCT 4 cut(s) 100, 190, 584, 684
Alw21I GWGCWC 1 cut(s) 686
AlwNI CAGNNNCTG 1 cut(s) 215
AoxI GGCC 3 cut(s) 70, 543, 796
ApeKI GCWGC 1 cut(s) 349
ApoI RAATTY 3 cut(s) 272, 607, 667
ArsI GACNNNNNNTTYG 2 cut(s) 485, 517
AspLEI GCGC 2 cut(s) 237, 773
AspS9I GGNCC 2 cut(s) 543, 576
AsuHPI GGTGA 1 cut(s) 77
AvaII GGWCC 1 cut(s) 576
BanII GRGCYC 1 cut(s) 686
BbsI GAAGAC 2 cut(s) 240, 633
Bbv12I GWGCWC 1 cut(s) 686
BbvI GCAGC 1 cut(s) 361
BccI CCATC 3 cut(s) 10, 62, 431
BcgI CGANNNNNNTGC 2 cut(s) 359, 393
BciT130I CCWGG 2 cut(s) 44, 198
BfaI CTAG 2 cut(s) 549, 806
BfmI CTRYAG 1 cut(s) 207
BisI GCNGC 2 cut(s) 73, 350
BlsI GCNGC 2 cut(s) 74, 351
Bme1390I CCNGG 2 cut(s) 44, 198
Bme18I GGWCC 1 cut(s) 576
BmgT120I GGNCC 2 cut(s) 543, 576
BmiI GGNNCC 1 cut(s) 578
BmrFI CCNGG 2 cut(s) 44, 198
BmrI ACTGGG 1 cut(s) 550
BmuI ACTGGG 1 cut(s) 550
BpiI GAAGAC 2 cut(s) 240, 633
BsaJI CCNNGG 2 cut(s) 73, 328
BsaXI ACNNNNNCTCC 2 cut(s) 546, 576
Bse1I ACTGG 2 cut(s) 499, 545
BseBI CCWGG 2 cut(s) 44, 198
BseDI CCNNGG 2 cut(s) 73, 328
BseGI GGATG 1 cut(s) 262
BseMII CTCAG 1 cut(s) 252
BseNI ACTGG 2 cut(s) 499, 545
BseRI GAGGAG 1 cut(s) 567
BseXI GCAGC 1 cut(s) 361
BsgI GTGCAG 1 cut(s) 761
Bsh1236I CGCG 1 cut(s) 75
BshFI GGCC 3 cut(s) 72, 545, 798
BsiHKAI GWGCWC 1 cut(s) 686
BslFI GGGAC 1 cut(s) 562
BsmFI GGGAC 1 cut(s) 562
BsmI GAATGC 2 cut(s) 415, 815
BsnI GGCC 3 cut(s) 72, 545, 798
Bsp1286I GDGCHC 1 cut(s) 686
BspACI CCGC 3 cut(s) 73, 75, 791
BspANI GGCC 3 cut(s) 72, 545, 798
BspCNI CTCAG 1 cut(s) 251
BspFNI CGCG 1 cut(s) 75
BspLI GGNNCC 1 cut(s) 578
BspMAI CTGCAG 1 cut(s) 211
BsrI ACTGG 2 cut(s) 499, 545
BssECI CCNNGG 2 cut(s) 73, 328
BssT1I CCWWGG 1 cut(s) 328
Bst2UI CCWGG 2 cut(s) 44, 198
Bst4CI ACNGT 1 cut(s) 217
BstC8I GCNNGC 4 cut(s) 98, 211, 628, 820
BstDEI CTNAG 2 cut(s) 238, 287
BstDSI CCRYGG 1 cut(s) 73
BstEII GGTNACC 1 cut(s) 4
BstF5I GGATG 1 cut(s) 262
BstFNI CGCG 1 cut(s) 75
BstHHI GCGC 2 cut(s) 237, 773
BstMWI GCNNNNNNNGC 4 cut(s) 187, 623, 790, 819
BstNI CCWGG 2 cut(s) 44, 198
BstPI GGTNACC 1 cut(s) 4
BstSCI CCNGG 2 cut(s) 42, 196
BstSFI CTRYAG 1 cut(s) 207
BstUI CGCG 1 cut(s) 75
BstV1I GCAGC 1 cut(s) 361
BstV2I GAAGAC 2 cut(s) 240, 633
BsuRI GGCC 3 cut(s) 72, 545, 798
BtgI CCRYGG 1 cut(s) 73
BtsCI GGATG 1 cut(s) 262
BtsI GCAGTG 1 cut(s) 813
BtsIMutI CAGTG 2 cut(s) 213, 813
Cac8I GCNNGC 4 cut(s) 98, 211, 628, 820
CaiI CAGNNNCTG 1 cut(s) 215
CfoI GCGC 2 cut(s) 237, 773
Cfr13I GGNCC 2 cut(s) 543, 576
Cfr42I CCGCGG 1 cut(s) 76
Csp6I GTAC 1 cut(s) 601
CviAII CATG 3 cut(s) 60, 505, 754
CviQI GTAC 1 cut(s) 601
DdeI CTNAG 2 cut(s) 238, 287
EaeI YGGCCR 1 cut(s) 70
Ecl136II GAGCTC 1 cut(s) 684
Eco130I CCWWGG 1 cut(s) 328
Eco147I AGGCCT 1 cut(s) 798
Eco24I GRGCYC 1 cut(s) 686
Eco47I GGWCC 1 cut(s) 576
Eco53kI GAGCTC 1 cut(s) 684
Eco91I GGTNACC 1 cut(s) 4
EcoICRI GAGCTC 1 cut(s) 684
EcoO65I GGTNACC 1 cut(s) 4
EcoRI GAATTC 1 cut(s) 272
EcoRII CCWGG 2 cut(s) 42, 196
EcoT14I CCWWGG 1 cut(s) 328
EcoT38I GRGCYC 1 cut(s) 686
ErhI CCWWGG 1 cut(s) 328
FaeI CATG 3 cut(s) 63, 508, 757
FalI AAGNNNNNCTT 2 cut(s) 714, 746
FaqI GGGAC 1 cut(s) 562
FatI CATG 3 cut(s) 59, 504, 753
FauI CCCGC 1 cut(s) 798
Fnu4HI GCNGC 2 cut(s) 73, 350
FokI GGATG 1 cut(s) 269
FriOI GRGCYC 1 cut(s) 686
Fsp4HI GCNGC 2 cut(s) 73, 350
FspBI CTAG 2 cut(s) 549, 806
FspI TGCGCA 1 cut(s) 772
GlaI GCGC 2 cut(s) 236, 772
GluI GCNGC 2 cut(s) 73, 350
HaeIII GGCC 3 cut(s) 72, 545, 798
HhaI GCGC 2 cut(s) 237, 773
Hin1II CATG 3 cut(s) 63, 508, 757
Hin6I GCGC 2 cut(s) 235, 771
HinP1I GCGC 2 cut(s) 235, 771
HindIII AAGCTT 1 cut(s) 188
HinfI GANTC 4 cut(s) 283, 470, 710, 786
HphI GGTGA 1 cut(s) 77
Hpy188I TCNGA 1 cut(s) 241
Hpy188III TCNNGA 3 cut(s) 460, 526, 806
HpyAV CCTTC 2 cut(s) 236, 606
HpyCH4III ACNGT 1 cut(s) 217
HpyCH4V TGCA 6 cut(s) 181, 209, 630, 660, 742, 813
HpyF10VI GCNNNNNNNGC 4 cut(s) 187, 623, 790, 819
HpyF3I CTNAG 2 cut(s) 238, 287
Hsp92II CATG 3 cut(s) 63, 508, 757
HspAI GCGC 2 cut(s) 235, 771
KspI CCGCGG 1 cut(s) 76
LmnI GCTCC 1 cut(s) 589
Lsp1109I GCAGC 1 cut(s) 361
MaeI CTAG 2 cut(s) 549, 806
MaeIII GTNAC 4 cut(s) 4, 55, 200, 217
MboII GAAGA 3 cut(s) 240, 633, 757
MfeI CAATTG 1 cut(s) 474
MhlI GDGCHC 1 cut(s) 686
MlyI GAGTC 2 cut(s) 479, 704
MseI TTAA 2 cut(s) 186, 832
MspA1I CMGCKG 1 cut(s) 75
MspR9I CCNGG 2 cut(s) 44, 198
MunI CAATTG 1 cut(s) 474
Mva1269I GAATGC 2 cut(s) 415, 815
MvaI CCWGG 2 cut(s) 44, 198
MvnI CGCG 1 cut(s) 75
MwoI GCNNNNNNNGC 4 cut(s) 187, 623, 790, 819
NlaIII CATG 3 cut(s) 63, 508, 757
NlaIV GGNNCC 1 cut(s) 578
NmuCI GTSAC 1 cut(s) 217
NsbI TGCGCA 1 cut(s) 772
PceI AGGCCT 1 cut(s) 798
PctI GAATGC 2 cut(s) 415, 815
PfeI GAWTC 2 cut(s) 283, 786
PkrI GCNGC 2 cut(s) 74, 351
PleI GAGTC 2 cut(s) 478, 704
PpsI GAGTC 2 cut(s) 478, 704
PsiI TTATAA 1 cut(s) 762
Psp124BI GAGCTC 1 cut(s) 686
Psp6I CCWGG 2 cut(s) 42, 196
PspEI GGTNACC 1 cut(s) 4
PspGI CCWGG 2 cut(s) 42, 196
PspN4I GGNNCC 1 cut(s) 578
PspPI GGNCC 2 cut(s) 543, 576
PstI CTGCAG 1 cut(s) 211
PstNI CAGNNNCTG 1 cut(s) 215
RsaI GTAC 1 cut(s) 602
RsaNI GTAC 1 cut(s) 601
SacI GAGCTC 1 cut(s) 686
SacII CCGCGG 1 cut(s) 76
SaqAI TTAA 2 cut(s) 186, 832
SatI GCNGC 2 cut(s) 73, 350
Sau96I GGNCC 2 cut(s) 543, 576
SchI GAGTC 2 cut(s) 479, 704
ScrFI CCNGG 2 cut(s) 44, 198
SduI GDGCHC 1 cut(s) 686
SfcI CTRYAG 1 cut(s) 207
Sfr303I CCGCGG 1 cut(s) 76
SgrBI CCGCGG 1 cut(s) 76
SinI GGWCC 1 cut(s) 576
SseBI AGGCCT 1 cut(s) 798
SsiI CCGC 3 cut(s) 73, 75, 791
SspMI CTAG 2 cut(s) 549, 806
SstI GAGCTC 1 cut(s) 686
StuI AGGCCT 1 cut(s) 798
StyD4I CCNGG 2 cut(s) 42, 196
StyI CCWWGG 1 cut(s) 328
TaaI ACNGT 1 cut(s) 217
TaqI TCGA 1 cut(s) 369
TauI GCSGC 1 cut(s) 75
TfiI GAWTC 2 cut(s) 283, 786
Tru1I TTAA 2 cut(s) 186, 832
Tru9I TTAA 2 cut(s) 186, 832
TscAI CASTG 2 cut(s) 220, 820
TseFI GTSAC 1 cut(s) 217
TseI GCWGC 1 cut(s) 349
Tsp45I GTSAC 1 cut(s) 217
TspDTI ATGAA 2 cut(s) 299, 474
TspRI CASTG 2 cut(s) 220, 820
VpaK11BI GGWCC 1 cut(s) 576
XapI RAATTY 3 cut(s) 272, 607, 667
XbaI TCTAGA 1 cut(s) 805
XspI CTAG 2 cut(s) 549, 806
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.