Rmu_sc0008058.1_g000030

Catalyzes xyloglucan endohydrolysis (XEH) and or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0008058.1
OG0000460
Physical Location & Seq
Reverse (-)
94461 .. 95543
1083 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0008058.1_g000030.1.cds

Sequence Viewer

Length: 858 bp
atggcttcatccagttcactaatgttgctgactattgctcttcttattagctctttcatggttgcctctgccggtaacttcaaccaagactttaagattacatggggggatggtcgagccaatatactcaacgacggtcaacttcttactctttcgctcgacaaagcctctggctctggttttgaatcccaaaacgaatatctattcggcaagattgacatgcagctcaaacttgttgccggaaactctgctggaactgtcactgcctattatttaaaatcagaagggtctacatgggatgagatagactttgagttcttgggaaacttgagtggtgacccttacattcttcacaccaatatcttctgccaaggcaaaggaaaccgagagcagcaattctacctctggttcgacccaactgctgatttccacacctattccattctctggaatccacagcgcattgttttttcagttgatggcactcccattagagagttcaagaatcaagagtcgaaaggtgttccattcctgaagagccaagcgatgagaatacattcgagcctctggaacgccgatgattgggcaacaagaggtggacttgtgaagacagattggagtgcagctcctttcactgcctcttacaggaattttaatgctgaagcttgcatttggtcttctggagcatcttcttgcagttcaactagtactaataatggtggttggctatcacaagagctggacagtacaagtcaagaaaggatgagatgggtgcagaagaactacatgatctacaattactgtgcggatgtcaagcgtttacctcagggcctccctgtagaatgcagcacttcctag

Protein Analysis

285

Amino Acids

31.79

Weight (kDa)

5.12

Isoelectric Point (pI)

36.34

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000460)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G25810 AT4G30270 AT5G57550 AT5G57560
fragaria_vesca FvH4_3g00833 FvH4_3g00840 FvH4_3g00850 FvH4_4g06700 FvH4_4g09230
malus_domestica MD13G1237300.v1.1 MD13G1268000.v1.1 MD13G1268900.v1.1 MD16G1267200.v1.1 MD16G1267300.v1.1
prunus_persica Prupe.1G069800_v2.0.a1 Prupe.1G088600_v2.0.a1 Prupe.1G088800_v2.0.a1 Prupe.1G088900_v2.0.a1 Prupe.1G089000_v2.0.a1
pyrus_communis pycom13g21020 pycom13g22350 pycom13g22380 pycom16g23630 pycom16g23640
rosa_chinensis RchiOBHm_Chr4g0398631 RchiOBHm_Chr4g0402891 RchiOBHm_Chr4g0402911 RchiOBHm_Chr4g0402921 RchiOBHm_Chr4g0402931 RchiOBHm_Chr4g0402951 RchiOBHm_Chr4g0402961 RchiOBHm_Chr4g0402981
rosa_laevigata RLG00000009031 RLG00000009033 RLG00000009034 RLG00000009035 RLG00000009039 RLG00000009040 RLG00000009339
rosa_multiflora Rmu_co8186292.1_g000001 Rmu_sc0000990.1_g000004 Rmu_sc0001023.1_g000002 Rmu_sc0001023.1_g000004 Rmu_sc0001023.1_g000007 Rmu_sc0001023.1_g000015 Rmu_sc0002178.1_g000007 Rmu_sc0002451.1_g000069 Rmu_sc0002451.1_g000079 Rmu_sc0002451.1_g000080 Rmu_sc0008058.1_g000026 Rmu_sc0008058.1_g000029 Rmu_sc0008058.1_g000030
rosa_roxburghii Rroxscaffold_5G00343590 Rroxscaffold_5G00347620 Rroxscaffold_5G00347630 Rroxscaffold_5G00347640 Rroxscaffold_5G00347650 Rroxscaffold_5G00347660 Rroxscaffold_5G00347670 Rroxscaffold_5G00347690
rosa_rugosa Rorug04G0009900 Rorug04G0039000 Rorug04G0039100 Rorug04G0039200 Rorug04G0039300 Rorug04G0039400 Rorug04G0039500 Rorug04G0039600 Rorug04G0039600 Rorug04G0040200
rosa_samantha Rh4AG087800 Rh4AG116500 Rh4AG116700 Rh4AG116800 Rh4AG116900 Rh4AG117000 Rh4AG117100 Rh4BG109300 Rh4BG109400 Rh4BG109500 Rh4BG109600 Rh4BG109700 Rh4BG109800 Rh4CG096100 Rh4CG123700 Rh4CG124200
rosa_wichuraiana Rw4G007280 Rw4G009320 Rw4G009340 Rw4G009370 Rw4G009380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 447
AccI GTMKAC 1 cut(s) 290
AciI CCGC 1 cut(s) 806
AcsI RAATTY 1 cut(s) 649
AcuI CTGAAG 2 cut(s) 554, 681
AfaI GTAC 2 cut(s) 709, 748
AfiI CCNNNNNNNGG 3 cut(s) 447, 582, 645
AgsI TTSAA 4 cut(s) 82, 185, 502, 702
AhlI ACTAGT 1 cut(s) 704
AluBI AGCT 5 cut(s) 51, 226, 626, 665, 739
AluI AGCT 5 cut(s) 51, 226, 626, 665, 739
AoxI GGCC 1 cut(s) 829
ApeKI GCWGC 4 cut(s) 223, 391, 623, 846
ApoI RAATTY 1 cut(s) 649
Asp700I GAANNNNTTC 1 cut(s) 556
AspLEI GCGC 1 cut(s) 462
AspS9I GGNCC 1 cut(s) 829
AsuHPI GGTGA 1 cut(s) 347
AxyI CCTNAGG 1 cut(s) 825
BbsI GAAGAC 2 cut(s) 614, 669
BbvI GCAGC 3 cut(s) 235, 403, 635
BccI CCATC 3 cut(s) 104, 473, 762
BcgI CGANNNNNNTGC 2 cut(s) 401, 435
BcuI ACTAGT 1 cut(s) 704
BfaI CTAG 2 cut(s) 705, 856
BfmI CTRYAG 1 cut(s) 837
BisI GCNGC 4 cut(s) 224, 392, 624, 847
BlsI GCNGC 4 cut(s) 225, 393, 625, 848
BmcAI AGTACT 1 cut(s) 709
BmgT120I GGNCC 1 cut(s) 829
BmsI GCATC 1 cut(s) 695
BpiI GAAGAC 2 cut(s) 614, 669
BplI GAGNNNNNCTC 2 cut(s) 610, 642
BpmI CTGGAG 1 cut(s) 702
BpuEI CTTGAG 1 cut(s) 349
BsaJI CCNNGG 1 cut(s) 370
Bsc4I CCNNNNNNNGG 3 cut(s) 447, 582, 645
Bse118I RCCGGY 1 cut(s) 71
Bse1I ACTGG 1 cut(s) 12
Bse21I CCTNAGG 1 cut(s) 825
BseDI CCNNGG 1 cut(s) 370
BseGI GGATG 5 cut(s) 8, 115, 304, 768, 814
BseLI CCNNNNNNNGG 3 cut(s) 447, 582, 645
BseMII CTCAG 1 cut(s) 839
BseNI ACTGG 1 cut(s) 12
BseXI GCAGC 3 cut(s) 235, 403, 635
BsgI GTGCAG 2 cut(s) 642, 794
BshFI GGCC 1 cut(s) 831
BsiSI CCGG 2 cut(s) 72, 240
BslI CCNNNNNNNGG 3 cut(s) 447, 582, 645
BsmI GAATGC 1 cut(s) 848
BsnI GGCC 1 cut(s) 831
Bsp143I GATC 1 cut(s) 789
BspACI CCGC 1 cut(s) 806
BspANI GGCC 1 cut(s) 831
BspCNI CTCAG 1 cut(s) 838
BspQI GCTCTTC 2 cut(s) 45, 530
BsrFI RCCGGY 1 cut(s) 71
BsrI ACTGG 1 cut(s) 12
BssAI RCCGGY 1 cut(s) 71
BssECI CCNNGG 1 cut(s) 370
BssMI GATC 1 cut(s) 789
BssT1I CCWWGG 1 cut(s) 370
Bst4CI ACNGT 4 cut(s) 137, 259, 746, 803
Bst6I CTCTTC 2 cut(s) 45, 530
BstC8I GCNNGC 1 cut(s) 667
BstDEI CTNAG 1 cut(s) 825
BstEII GGTNACC 1 cut(s) 335
BstENI CCTNNNNNAGG 1 cut(s) 643
BstF5I GGATG 5 cut(s) 8, 115, 304, 768, 814
BstHHI GCGC 1 cut(s) 462
BstKTI GATC 1 cut(s) 792
BstMBI GATC 1 cut(s) 789
BstNSI RCATGY 1 cut(s) 223
BstPI GGTNACC 1 cut(s) 335
BstSFI CTRYAG 1 cut(s) 837
BstV1I GCAGC 3 cut(s) 235, 403, 635
BstV2I GAAGAC 2 cut(s) 614, 669
Bsu36I CCTNAGG 1 cut(s) 825
BsuRI GGCC 1 cut(s) 831
BtgZI GCGATG 1 cut(s) 560
BtsCI GGATG 5 cut(s) 8, 115, 304, 768, 814
BtsI GCAGTG 2 cut(s) 261, 633
BtsIMutI CAGTG 2 cut(s) 261, 633
Cac8I GCNNGC 1 cut(s) 667
CfoI GCGC 1 cut(s) 462
Cfr10I RCCGGY 1 cut(s) 71
Cfr13I GGNCC 1 cut(s) 829
Csp6I GTAC 2 cut(s) 708, 747
CviAII CATG 5 cut(s) 58, 102, 220, 294, 787
CviQI GTAC 2 cut(s) 708, 747
DdeI CTNAG 1 cut(s) 825
DpnI GATC 1 cut(s) 791
DpnII GATC 1 cut(s) 789
DraI TTTAAA 1 cut(s) 276
Eam1104I CTCTTC 2 cut(s) 45, 530
EarI CTCTTC 2 cut(s) 45, 530
Eco130I CCWWGG 1 cut(s) 370
Eco57I CTGAAG 2 cut(s) 554, 681
Eco81I CCTNAGG 1 cut(s) 825
Eco91I GGTNACC 1 cut(s) 335
EcoNI CCTNNNNNAGG 1 cut(s) 643
EcoO109I RGGNCCY 1 cut(s) 829
EcoO65I GGTNACC 1 cut(s) 335
EcoT14I CCWWGG 1 cut(s) 370
ErhI CCWWGG 1 cut(s) 370
FaeI CATG 5 cut(s) 61, 105, 223, 297, 790
FaiI YATR 6 cut(s) 59, 103, 125, 221, 295, 788
FatI CATG 5 cut(s) 57, 101, 219, 293, 786
FblI GTMKAC 1 cut(s) 290
Fnu4HI GCNGC 4 cut(s) 224, 392, 624, 847
FokI GGATG 4 cut(s) 122, 311, 775, 821
Fsp4HI GCNGC 4 cut(s) 224, 392, 624, 847
FspBI CTAG 2 cut(s) 705, 856
GlaI GCGC 1 cut(s) 461
GluI GCNGC 4 cut(s) 224, 392, 624, 847
GsuI CTGGAG 1 cut(s) 702
HaeIII GGCC 1 cut(s) 831
HapII CCGG 2 cut(s) 72, 240
HhaI GCGC 1 cut(s) 462
Hin1II CATG 5 cut(s) 61, 105, 223, 297, 790
Hin6I GCGC 1 cut(s) 460
HinP1I GCGC 1 cut(s) 460
HincII GTYRAC 1 cut(s) 140
HindII GTYRAC 1 cut(s) 140
HindIII AAGCTT 1 cut(s) 663
HinfI GANTC 4 cut(s) 185, 451, 505, 512
HpaII CCGG 2 cut(s) 72, 240
HphI GGTGA 1 cut(s) 347
Hpy166II GTNNAC 5 cut(s) 17, 140, 291, 599, 821
Hpy188I TCNGA 1 cut(s) 283
Hpy188III TCNNGA 7 cut(s) 448, 502, 509, 532, 568, 681, 755
Hpy8I GTNNAC 5 cut(s) 17, 140, 291, 599, 821
Hpy99I CGWCG 1 cut(s) 137
HpyAV CCTTC 1 cut(s) 278
HpyCH4III ACNGT 4 cut(s) 137, 259, 746, 803
HpyCH4V TGCA 6 cut(s) 223, 623, 669, 696, 775, 846
HpyF3I CTNAG 1 cut(s) 825
Hsp92II CATG 5 cut(s) 61, 105, 223, 297, 790
HspAI GCGC 1 cut(s) 460
Kzo9I GATC 1 cut(s) 789
LguI GCTCTTC 2 cut(s) 45, 530
LmnI GCTCC 2 cut(s) 631, 683
Lsp1109I GCAGC 3 cut(s) 235, 403, 635
LweI GCATC 1 cut(s) 695
MaeI CTAG 2 cut(s) 705, 856
MaeIII GTNAC 3 cut(s) 74, 259, 335
MalI GATC 1 cut(s) 791
MboI GATC 1 cut(s) 789
MboII GAAGA 8 cut(s) 32, 341, 355, 547, 619, 669, 681, 790
MluCI AATT 3 cut(s) 395, 649, 796
MlyI GAGTC 1 cut(s) 521
MnlI CCTC 8 cut(s) 76, 178, 413, 575, 587, 649, 834, 842
MroXI GAANNNNTTC 1 cut(s) 556
MseI TTAA 3 cut(s) 93, 275, 654
MspI CCGG 2 cut(s) 72, 240
Mva1269I GAATGC 1 cut(s) 848
NdeII GATC 1 cut(s) 789
NlaIII CATG 5 cut(s) 61, 105, 223, 297, 790
NmuCI GTSAC 2 cut(s) 259, 335
NspI RCATGY 1 cut(s) 223
PciSI GCTCTTC 2 cut(s) 45, 530
PctI GAATGC 1 cut(s) 848
PdmI GAANNNNTTC 1 cut(s) 556
PfeI GAWTC 3 cut(s) 185, 451, 505
PflMI CCANNNNNTGG 1 cut(s) 447
PkrI GCNGC 4 cut(s) 225, 393, 625, 848
PleI GAGTC 1 cut(s) 520
PpsI GAGTC 1 cut(s) 520
PspEI GGTNACC 1 cut(s) 335
PspPI GGNCC 1 cut(s) 829
RsaI GTAC 2 cut(s) 709, 748
RsaNI GTAC 2 cut(s) 708, 747
SapI GCTCTTC 2 cut(s) 45, 530
SaqAI TTAA 3 cut(s) 93, 275, 654
SatI GCNGC 4 cut(s) 224, 392, 624, 847
Sau3AI GATC 1 cut(s) 789
Sau96I GGNCC 1 cut(s) 829
ScaI AGTACT 1 cut(s) 709
SchI GAGTC 1 cut(s) 521
SfaNI GCATC 1 cut(s) 695
SfcI CTRYAG 1 cut(s) 837
SmlI CTYRAG 1 cut(s) 328
SmoI CTYRAG 1 cut(s) 328
SpeI ACTAGT 1 cut(s) 704
Sse9I AATT 3 cut(s) 395, 649, 796
SsiI CCGC 1 cut(s) 806
SspMI CTAG 2 cut(s) 705, 856
StyI CCWWGG 1 cut(s) 370
TaaI ACNGT 4 cut(s) 137, 259, 746, 803
TaqI TCGA 5 cut(s) 115, 159, 411, 515, 560
TasI AATT 3 cut(s) 395, 649, 796
TatI WGTACW 2 cut(s) 707, 746
TfiI GAWTC 3 cut(s) 185, 451, 505
Tru1I TTAA 3 cut(s) 93, 275, 654
Tru9I TTAA 3 cut(s) 93, 275, 654
TscAI CASTG 2 cut(s) 268, 640
TseFI GTSAC 2 cut(s) 259, 335
TseI GCWGC 4 cut(s) 223, 391, 623, 846
Tsp45I GTSAC 2 cut(s) 259, 335
TspDTI ATGAA 1 cut(s) 46
TspRI CASTG 2 cut(s) 268, 640
Van91I CCANNNNNTGG 1 cut(s) 447
XagI CCTNNNNNAGG 1 cut(s) 643
XapI RAATTY 1 cut(s) 649
XceI RCATGY 1 cut(s) 223
XmiI GTMKAC 1 cut(s) 290
XmnI GAANNNNTTC 1 cut(s) 556
XspI CTAG 2 cut(s) 705, 856
ZrmI AGTACT 1 cut(s) 709
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.