MD16G1267200.v1.1

Catalyzes xyloglucan endohydrolysis (XEH) and or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0000460
Physical Location & Seq
Reverse (-)
34159056 .. 34160286
1231 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1267200.v1.1.491

Sequence Viewer

Length: 870 bp
ATGGCTTCCTACACTACAACGCTTATTCTGTTACCAATATTTCTTCTCAGCTTTGTGGTGGCTCCTGCCTCTGCTGCCGACTTAAACCAAGACTTTGATATTACATGGGGAGACGGACGTGCTAAGATTCTCAACAACGGCGAGCTTCTTACCCTTTCTCTCGACAAAGCCTCCGGCTCTGGGTTTCAATCCAAACACGAGTATTTGTTCGGCAAGATCGACATGCAGCTCAAGCTTGTCCCTGGAAACTCCGCTGGCACTGTCACAGCCTACTACTTATCATCAAAAGGATCAACATGGGATGAGATAGACTTTGAGTTCTTGGGGAACTTAAGTGGAGACCCTTACATTCTCCACACCAATGTCTTCAGCCAAGGCAAAGGCAACAGAGAGCAGCAATTCTATCTCTGGTTTGACCCAACTGCCGACTTCCACACATATTCCATCCTCTGGAACTCCCAGCGCATTATATTCTCAGTAGATGGAACTCCCATCAGAGAGTTCAAGAACCAAGAGTCGAACGGCGTTCCGTTTCCGAAAAGCCAGCCGATGAGGATATACTCGAGCCTGTGGAATGCCGATGATTGGGCAACGAGGGGAGGACTTGTGAAGACCGATTGGAGCCAGGCTCCTTTCACCGCCTCTTACAGAAATTTCAGTGCTGAAGCTTGCGTCTGGACTTCTGGAGCCTCCTCTTGCAGTTCTGCAACTTCAAGTAATGCTAACAATGGAGCTTGGCTTTCTCAAGACTTGGACTCTACGAGCCAAGACAGGCTGAAATGGGTGCAGACCAACTACATGATTTACAATTACTGTGCTGACACCAAGAGATTTCCCCAAGGCCTCCCGGTGGAATGCACCGCTTCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
Glyco_hydro_16 XET_C

Protein Analysis

290

Amino Acids

32.17

Weight (kDa)

4.96

Isoelectric Point (pI)

33.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_16 PF00722 29 - 208 1.5e-68 Glycosyl hydrolases family 16
XET_C PF06955 242 - 286 6.3e-21 Xyloglucan endo-transglycosylase (XET) C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000460)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G25810 AT4G30270 AT5G57550 AT5G57560
fragaria_vesca FvH4_3g00833 FvH4_3g00840 FvH4_3g00850 FvH4_4g06700 FvH4_4g09230
malus_domestica MD13G1237300.v1.1 MD13G1268000.v1.1 MD13G1268900.v1.1 MD16G1267200.v1.1 MD16G1267300.v1.1
prunus_persica Prupe.1G069800_v2.0.a1 Prupe.1G088600_v2.0.a1 Prupe.1G088800_v2.0.a1 Prupe.1G088900_v2.0.a1 Prupe.1G089000_v2.0.a1
pyrus_communis pycom13g21020 pycom13g22350 pycom13g22380 pycom16g23630 pycom16g23640
rosa_chinensis RchiOBHm_Chr4g0398631 RchiOBHm_Chr4g0402891 RchiOBHm_Chr4g0402911 RchiOBHm_Chr4g0402921 RchiOBHm_Chr4g0402931 RchiOBHm_Chr4g0402951 RchiOBHm_Chr4g0402961 RchiOBHm_Chr4g0402981
rosa_laevigata RLG00000009031 RLG00000009033 RLG00000009034 RLG00000009035 RLG00000009039 RLG00000009040 RLG00000009339
rosa_multiflora Rmu_co8186292.1_g000001 Rmu_sc0000990.1_g000004 Rmu_sc0001023.1_g000002 Rmu_sc0001023.1_g000004 Rmu_sc0001023.1_g000007 Rmu_sc0001023.1_g000015 Rmu_sc0002178.1_g000007 Rmu_sc0002451.1_g000069 Rmu_sc0002451.1_g000079 Rmu_sc0002451.1_g000080 Rmu_sc0008058.1_g000026 Rmu_sc0008058.1_g000029 Rmu_sc0008058.1_g000030
rosa_roxburghii Rroxscaffold_5G00343590 Rroxscaffold_5G00347620 Rroxscaffold_5G00347630 Rroxscaffold_5G00347640 Rroxscaffold_5G00347650 Rroxscaffold_5G00347660 Rroxscaffold_5G00347670 Rroxscaffold_5G00347690
rosa_rugosa Rorug04G0009900 Rorug04G0039000 Rorug04G0039100 Rorug04G0039200 Rorug04G0039300 Rorug04G0039400 Rorug04G0039500 Rorug04G0039600 Rorug04G0039600 Rorug04G0040200
rosa_samantha Rh4AG087800 Rh4AG116500 Rh4AG116700 Rh4AG116800 Rh4AG116900 Rh4AG117000 Rh4AG117100 Rh4BG109300 Rh4BG109400 Rh4BG109500 Rh4BG109600 Rh4BG109700 Rh4BG109800 Rh4CG096100 Rh4CG123700 Rh4CG124200
rosa_wichuraiana Rw4G007280 Rw4G009320 Rw4G009340 Rw4G009370 Rw4G009380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 450
AciI CCGC 3 cut(s) 252, 639, 861
AclWI GGATC 1 cut(s) 298
AcsI RAATTY 1 cut(s) 652
AcuI CTGAAG 2 cut(s) 352, 684
AfiI CCNNNNNNNGG 4 cut(s) 180, 450, 585, 850
AflII CTTAAG 1 cut(s) 331
AgsI TTSAA 3 cut(s) 188, 505, 714
AjiI CACGTC 1 cut(s) 119
AjnI CCWGG 2 cut(s) 241, 624
AluBI AGCT 6 cut(s) 51, 145, 229, 235, 668, 734
AluI AGCT 6 cut(s) 51, 145, 229, 235, 668, 734
Alw26I GTCTC 2 cut(s) 105, 333
AlwI GGATC 1 cut(s) 298
Ama87I CYCGRG 1 cut(s) 562
AoxI GGCC 1 cut(s) 841
ApeKI GCWGC 3 cut(s) 74, 226, 394
ApoI RAATTY 1 cut(s) 652
AspLEI GCGC 1 cut(s) 465
AsuC2I CCSGG 1 cut(s) 848
AsuHPI GGTGA 1 cut(s) 628
AvaI CYCGRG 1 cut(s) 562
BauI CACGAG 1 cut(s) 197
BbsI GAAGAC 2 cut(s) 358, 617
BbvI GCAGC 3 cut(s) 61, 238, 406
BccI CCATC 3 cut(s) 452, 476, 500
BceAI ACGGC 2 cut(s) 154, 538
BciT130I CCWGG 2 cut(s) 243, 626
BcnI CCSGG 1 cut(s) 848
BcoDI GTCTC 2 cut(s) 105, 333
BfrI CTTAAG 1 cut(s) 331
BisI GCNGC 3 cut(s) 75, 227, 395
BlsI GCNGC 3 cut(s) 76, 228, 396
Bme1390I CCNGG 3 cut(s) 243, 626, 848
BmeT110I CYCGRG 1 cut(s) 562
BmgBI CACGTC 1 cut(s) 119
BmiI GGNNCC 4 cut(s) 63, 623, 630, 688
BmrFI CCNGG 3 cut(s) 243, 626, 848
BpiI GAAGAC 2 cut(s) 358, 617
BplI GAGNNNNNCTC 2 cut(s) 613, 645
BpmI CTGGAG 1 cut(s) 705
BpuEI CTTGAG 2 cut(s) 215, 729
BpuMI CCSGG 1 cut(s) 848
BsaI GGTCTC 1 cut(s) 333
BsaJI CCNNGG 3 cut(s) 241, 373, 838
BsaXI ACNNNNNCTCC 4 cut(s) 155, 185, 591, 621
Bsc4I CCNNNNNNNGG 4 cut(s) 180, 450, 585, 850
BseBI CCWGG 2 cut(s) 243, 626
BseDI CCNNGG 3 cut(s) 241, 373, 838
BseGI GGATG 2 cut(s) 307, 444
BseLI CCNNNNNNNGG 4 cut(s) 180, 450, 585, 850
BseMII CTCAG 2 cut(s) 61, 489
BseRI GAGGAG 1 cut(s) 682
BseXI GCAGC 3 cut(s) 61, 238, 406
BseYI CCCAGC 1 cut(s) 459
BsgI GTGCAG 1 cut(s) 806
BshFI GGCC 1 cut(s) 843
BsiHKCI CYCGRG 1 cut(s) 562
BsiSI CCGG 2 cut(s) 174, 848
BslFI GGGAC 1 cut(s) 224
BslI CCNNNNNNNGG 4 cut(s) 180, 450, 585, 850
BsmAI GTCTC 2 cut(s) 105, 333
BsmBI CGTCTC 1 cut(s) 105
BsmFI GGGAC 1 cut(s) 224
BsmI GAATGC 2 cut(s) 580, 860
BsnI GGCC 1 cut(s) 843
Bso31I GGTCTC 1 cut(s) 333
BsoBI CYCGRG 1 cut(s) 562
Bsp143I GATC 2 cut(s) 216, 290
BspACI CCGC 3 cut(s) 252, 639, 861
BspANI GGCC 1 cut(s) 843
BspCNI CTCAG 2 cut(s) 60, 488
BspLI GGNNCC 4 cut(s) 63, 623, 630, 688
BspPI GGATC 1 cut(s) 298
BspTI CTTAAG 1 cut(s) 331
BspTNI GGTCTC 1 cut(s) 333
BssECI CCNNGG 3 cut(s) 241, 373, 838
BssMI GATC 2 cut(s) 216, 290
BssSI CACGAG 1 cut(s) 197
BssT1I CCWWGG 2 cut(s) 373, 838
Bst2BI CACGAG 1 cut(s) 197
Bst2UI CCWGG 2 cut(s) 243, 626
Bst4CI ACNGT 2 cut(s) 262, 815
BstAFI CTTAAG 1 cut(s) 331
BstC8I GCNNGC 4 cut(s) 143, 256, 545, 670
BstDEI CTNAG 3 cut(s) 47, 123, 475
BstF5I GGATG 2 cut(s) 307, 444
BstHHI GCGC 1 cut(s) 465
BstKTI GATC 2 cut(s) 219, 293
BstMAI GTCTC 2 cut(s) 105, 333
BstMBI GATC 2 cut(s) 216, 290
BstMWI GCNNNNNNNGC 2 cut(s) 74, 232
BstNI CCWGG 2 cut(s) 243, 626
BstNSI RCATGY 1 cut(s) 226
BstSCI CCNGG 3 cut(s) 241, 624, 846
BstV1I GCAGC 3 cut(s) 61, 238, 406
BstV2I GAAGAC 2 cut(s) 358, 617
BsuRI GGCC 1 cut(s) 843
BtrI CACGTC 1 cut(s) 119
BtsCI GGATG 2 cut(s) 307, 444
BtsIMutI CAGTG 2 cut(s) 258, 664
Cac8I GCNNGC 4 cut(s) 143, 256, 545, 670
CfoI GCGC 1 cut(s) 465
CseI GACGC 1 cut(s) 661
CviAII CATG 4 cut(s) 105, 223, 297, 799
DdeI CTNAG 3 cut(s) 47, 123, 475
DpnI GATC 2 cut(s) 218, 292
DpnII GATC 2 cut(s) 216, 290
Eco130I CCWWGG 2 cut(s) 373, 838
Eco147I AGGCCT 1 cut(s) 843
Eco31I GGTCTC 1 cut(s) 333
Eco57I CTGAAG 2 cut(s) 352, 684
Eco88I CYCGRG 1 cut(s) 562
EcoRII CCWGG 2 cut(s) 241, 624
EcoT14I CCWWGG 2 cut(s) 373, 838
ErhI CCWWGG 2 cut(s) 373, 838
Esp3I CGTCTC 1 cut(s) 105
FaeI CATG 4 cut(s) 108, 226, 300, 802
FaiI YATR 8 cut(s) 106, 224, 298, 439, 470, 559, 800, 868
FaqI GGGAC 1 cut(s) 224
FatI CATG 4 cut(s) 104, 222, 296, 798
Fnu4HI GCNGC 3 cut(s) 75, 227, 395
FokI GGATG 2 cut(s) 314, 431
Fsp4HI GCNGC 3 cut(s) 75, 227, 395
GlaI GCGC 1 cut(s) 464
GluI GCNGC 3 cut(s) 75, 227, 395
GsaI CCCAGC 1 cut(s) 463
GsuI CTGGAG 1 cut(s) 705
HaeIII GGCC 1 cut(s) 843
HapII CCGG 2 cut(s) 174, 848
HgaI GACGC 1 cut(s) 661
HhaI GCGC 1 cut(s) 465
Hin1II CATG 4 cut(s) 108, 226, 300, 802
Hin6I GCGC 1 cut(s) 463
HinP1I GCGC 1 cut(s) 463
HindIII AAGCTT 2 cut(s) 233, 666
HinfI GANTC 3 cut(s) 127, 515, 755
HpaII CCGG 2 cut(s) 174, 848
HphI GGTGA 1 cut(s) 628
Hpy188I TCNGA 2 cut(s) 497, 537
Hpy188III TCNNGA 6 cut(s) 161, 451, 505, 676, 684, 746
HpyCH4III ACNGT 2 cut(s) 262, 815
HpyCH4IV ACGT 1 cut(s) 118
HpyCH4V TGCA 5 cut(s) 226, 699, 707, 787, 858
HpyF10VI GCNNNNNNNGC 2 cut(s) 74, 232
HpyF3I CTNAG 3 cut(s) 47, 123, 475
HpySE526I ACGT 1 cut(s) 118
Hsp92II CATG 4 cut(s) 108, 226, 300, 802
HspAI GCGC 1 cut(s) 463
Kzo9I GATC 2 cut(s) 216, 290
LmnI GCTCC 5 cut(s) 67, 621, 634, 686, 731
Lsp1109I GCAGC 3 cut(s) 61, 238, 406
MaeII ACGT 1 cut(s) 118
MaeIII GTNAC 2 cut(s) 30, 262
MalI GATC 2 cut(s) 218, 292
MboI GATC 2 cut(s) 216, 290
MboII GAAGA 3 cut(s) 35, 358, 622
MluCI AATT 3 cut(s) 398, 652, 808
MlyI GAGTC 2 cut(s) 524, 749
MseI TTAA 2 cut(s) 83, 332
MslI CAYNNNNRTG 1 cut(s) 360
MspA1I CMGCKG 1 cut(s) 254
MspCI CTTAAG 1 cut(s) 331
MspI CCGG 2 cut(s) 174, 848
MspR9I CCNGG 3 cut(s) 243, 626, 848
Mva1269I GAATGC 2 cut(s) 580, 860
MvaI CCWGG 2 cut(s) 243, 626
MwoI GCNNNNNNNGC 2 cut(s) 74, 232
NciI CCSGG 1 cut(s) 848
NdeII GATC 2 cut(s) 216, 290
NlaIII CATG 4 cut(s) 108, 226, 300, 802
NlaIV GGNNCC 4 cut(s) 63, 623, 630, 688
NmuCI GTSAC 1 cut(s) 262
NspI RCATGY 1 cut(s) 226
PaeR7I CTCGAG 1 cut(s) 562
PceI AGGCCT 1 cut(s) 843
PcsI WCGNNNNNNNCGW 1 cut(s) 216
PctI GAATGC 2 cut(s) 580, 860
PfeI GAWTC 1 cut(s) 127
PflMI CCANNNNNTGG 1 cut(s) 450
PkrI GCNGC 3 cut(s) 76, 228, 396
PleI GAGTC 2 cut(s) 523, 749
PpsI GAGTC 2 cut(s) 523, 749
Psp6I CCWGG 2 cut(s) 241, 624
PspFI CCCAGC 1 cut(s) 459
PspGI CCWGG 2 cut(s) 241, 624
PspN4I GGNNCC 4 cut(s) 63, 623, 630, 688
PspXI VCTCGAGB 1 cut(s) 562
RseI CAYNNNNRTG 1 cut(s) 360
SaqAI TTAA 2 cut(s) 83, 332
SatI GCNGC 3 cut(s) 75, 227, 395
Sau3AI GATC 2 cut(s) 216, 290
SchI GAGTC 2 cut(s) 524, 749
ScrFI CCNGG 3 cut(s) 243, 626, 848
SetI ASST 7 cut(s) 53, 121, 147, 231, 237, 670, 736
Sfr274I CTCGAG 1 cut(s) 562
SlaI CTCGAG 1 cut(s) 562
SmiMI CAYNNNNRTG 1 cut(s) 360
SmlI CTYRAG 4 cut(s) 230, 331, 562, 744
SmoI CTYRAG 4 cut(s) 230, 331, 562, 744
Sse9I AATT 3 cut(s) 398, 652, 808
SseBI AGGCCT 1 cut(s) 843
SsiI CCGC 3 cut(s) 252, 639, 861
SspI AATATT 1 cut(s) 39
StuI AGGCCT 1 cut(s) 843
StyD4I CCNGG 3 cut(s) 241, 624, 846
StyI CCWWGG 2 cut(s) 373, 838
TaaI ACNGT 2 cut(s) 262, 815
TaiI ACGT 1 cut(s) 121
TaqI TCGA 4 cut(s) 162, 219, 518, 563
TaqII GACCGA 1 cut(s) 629
TasI AATT 3 cut(s) 398, 652, 808
TfiI GAWTC 1 cut(s) 127
Tru1I TTAA 2 cut(s) 83, 332
Tru9I TTAA 2 cut(s) 83, 332
TscAI CASTG 2 cut(s) 265, 664
TseFI GTSAC 1 cut(s) 262
TseI GCWGC 3 cut(s) 74, 226, 394
Tsp45I GTSAC 1 cut(s) 262
TspDTI ATGAA 1 cut(s) 855
TspGWI ACGGA 2 cut(s) 129, 519
TspRI CASTG 2 cut(s) 265, 664
Van91I CCANNNNNTGG 1 cut(s) 450
Vha464I CTTAAG 1 cut(s) 331
XapI RAATTY 1 cut(s) 652
XceI RCATGY 1 cut(s) 226
XhoI CTCGAG 1 cut(s) 562
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.