MD13G1237300.v1.1

Catalyzes xyloglucan endohydrolysis (XEH) and or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0000460
Physical Location & Seq
Reverse (-)
24139197 .. 24140931
1735 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1237300.v1.1.491

Sequence Viewer

Length: 849 bp
ATGACTAAGATATTCCTTGTTCTAGTACTTCTGGTGTTCAATGTTTTGGTGGCGACGTCGGCCGGAAACTTCAACCAAGATTTTGACATAACATGGGGCGATGGTCGGGCAAAAATACTCGACAACGGGCAGAGTCTTACGCTGTCCCTGGACAAGACATCAGGGTCGGGGTTTCAGTCGAAGAATCAGTACTTGTTCGGAAAAATTGATATGCAGATAAAGCTTGTGCCTGGTAACTCTGCTGGCACGGTTACAGCTTACTATCTATCATCATTGGGTTCTACTCATGACGAAATAGACTTCGAGTTTCTAGGCAACCTAAGTGGAGACCCTTATATTCTGCACACAAATATTTTCACACAAGGGAAGGGGAATAGGGAGCAACAGTTCTACCTTTGGTTTGATCCCACCAAAGACTTCCATACCTACTCCATCTTGTGGAACCCTCAAACCATCATCTTCTCAGTAGATGGAACACCCATTAGAGAGTTCAAGAACTTAGAATCTAAGGGAGTCTCTTTCCCAAAGGACCAAGGCATGTGGATATACTCAAGCCTATGGAATGCAGACGATTGGGCCACACGCGGCGGACTTGTGAAGACGGATTGGAGCAAAGCCCCCTTCACAGCCTCCTACAGAAACTTCAACGCCCAAGCTTGCACTTGGTCTTCAGGCACTTCCCGTTGTCCTTCAAAGTCCCCGAACGAGTCGAGCTGGTTAACTCAGTCACTCGATTCCACAGGGCAAGAGAGAATCAAATGGGTTCAGAAGAACTACATGATTTACAACTACTGCAGAGATACCAAGCGCTTCCCGCAAGGATTTCCTCCTGAGTGCTCTGCTGCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
Glyco_hydro_16 XET_C

Protein Analysis

283

Amino Acids

31.86

Weight (kDa)

6.82

Isoelectric Point (pI)

34.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_16 PF00722 24 - 205 1.2e-67 Glycosyl hydrolases family 16
XET_C PF06955 235 - 279 1.6e-22 Xyloglucan endo-transglycosylase (XET) C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000460)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G25810 AT4G30270 AT5G57550 AT5G57560
fragaria_vesca FvH4_3g00833 FvH4_3g00840 FvH4_3g00850 FvH4_4g06700 FvH4_4g09230
malus_domestica MD13G1237300.v1.1 MD13G1268000.v1.1 MD13G1268900.v1.1 MD16G1267200.v1.1 MD16G1267300.v1.1
prunus_persica Prupe.1G069800_v2.0.a1 Prupe.1G088600_v2.0.a1 Prupe.1G088800_v2.0.a1 Prupe.1G088900_v2.0.a1 Prupe.1G089000_v2.0.a1
pyrus_communis pycom13g21020 pycom13g22350 pycom13g22380 pycom16g23630 pycom16g23640
rosa_chinensis RchiOBHm_Chr4g0398631 RchiOBHm_Chr4g0402891 RchiOBHm_Chr4g0402911 RchiOBHm_Chr4g0402921 RchiOBHm_Chr4g0402931 RchiOBHm_Chr4g0402951 RchiOBHm_Chr4g0402961 RchiOBHm_Chr4g0402981
rosa_laevigata RLG00000009031 RLG00000009033 RLG00000009034 RLG00000009035 RLG00000009039 RLG00000009040 RLG00000009339
rosa_multiflora Rmu_co8186292.1_g000001 Rmu_sc0000990.1_g000004 Rmu_sc0001023.1_g000002 Rmu_sc0001023.1_g000004 Rmu_sc0001023.1_g000007 Rmu_sc0001023.1_g000015 Rmu_sc0002178.1_g000007 Rmu_sc0002451.1_g000069 Rmu_sc0002451.1_g000079 Rmu_sc0002451.1_g000080 Rmu_sc0008058.1_g000026 Rmu_sc0008058.1_g000029 Rmu_sc0008058.1_g000030
rosa_roxburghii Rroxscaffold_5G00343590 Rroxscaffold_5G00347620 Rroxscaffold_5G00347630 Rroxscaffold_5G00347640 Rroxscaffold_5G00347650 Rroxscaffold_5G00347660 Rroxscaffold_5G00347670 Rroxscaffold_5G00347690
rosa_rugosa Rorug04G0009900 Rorug04G0039000 Rorug04G0039100 Rorug04G0039200 Rorug04G0039300 Rorug04G0039400 Rorug04G0039500 Rorug04G0039600 Rorug04G0039600 Rorug04G0040200
rosa_samantha Rh4AG087800 Rh4AG116500 Rh4AG116700 Rh4AG116800 Rh4AG116900 Rh4AG117000 Rh4AG117100 Rh4BG109300 Rh4BG109400 Rh4BG109500 Rh4BG109600 Rh4BG109700 Rh4BG109800 Rh4CG096100 Rh4CG123700 Rh4CG124200
rosa_wichuraiana Rw4G007280 Rw4G009320 Rw4G009340 Rw4G009370 Rw4G009380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 163
AatII GACGTC 1 cut(s) 59
AccB7I CCANNNNNTGG 1 cut(s) 438
AccII CGCG 1 cut(s) 585
AciI CCGC 3 cut(s) 585, 588, 815
AclWI GGATC 1 cut(s) 398
AcoI YGGCCR 1 cut(s) 60
AcuI CTGAAG 1 cut(s) 654
AcyI GRCGYC 1 cut(s) 56
AfaI GTAC 2 cut(s) 27, 191
AfeI AGCGCT 1 cut(s) 809
AfiI CCNNNNNNNGG 1 cut(s) 438
AgsI TTSAA 5 cut(s) 40, 73, 493, 646, 693
AjnI CCWGG 2 cut(s) 147, 229
AloI GAACNNNNNNTCC 2 cut(s) 371, 403
AluBI AGCT 4 cut(s) 223, 257, 656, 714
AluI AGCT 4 cut(s) 223, 257, 656, 714
Alw21I GWGCWC 1 cut(s) 839
Alw26I GTCTC 2 cut(s) 321, 520
AlwI GGATC 1 cut(s) 398
Aor51HI AGCGCT 1 cut(s) 809
AoxI GGCC 2 cut(s) 60, 576
ApeKI GCWGC 1 cut(s) 842
AspLEI GCGC 1 cut(s) 810
AspS9I GGNCC 2 cut(s) 529, 576
AvaII GGWCC 1 cut(s) 529
BarI GAAGNNNNNNTAC 2 cut(s) 173, 205
BbsI GAAGAC 2 cut(s) 605, 660
Bbv12I GWGCWC 1 cut(s) 839
BbvI GCAGC 1 cut(s) 829
BccI CCATC 4 cut(s) 95, 440, 461, 464
BciT130I CCWGG 2 cut(s) 149, 231
BcoDI GTCTC 2 cut(s) 321, 520
BfaI CTAG 2 cut(s) 23, 311
BfmI CTRYAG 2 cut(s) 634, 793
BfoI RGCGCY 1 cut(s) 811
BisI GCNGC 2 cut(s) 586, 843
BlsI GCNGC 2 cut(s) 587, 844
BmcAI AGTACT 2 cut(s) 27, 191
Bme1390I CCNGG 2 cut(s) 149, 231
Bme18I GGWCC 1 cut(s) 529
BmgT120I GGNCC 2 cut(s) 529, 576
BmiI GGNNCC 1 cut(s) 443
BmrFI CCNGG 2 cut(s) 149, 231
BpiI GAAGAC 2 cut(s) 605, 660
BpuEI CTTGAG 1 cut(s) 535
BsaHI GRCGYC 1 cut(s) 56
BsaI GGTCTC 1 cut(s) 321
BsaJI CCNNGG 2 cut(s) 147, 532
BsaXI ACNNNNNCTCC 2 cut(s) 371, 401
Bsc4I CCNNNNNNNGG 1 cut(s) 438
BseBI CCWGG 2 cut(s) 149, 231
BseDI CCNNGG 2 cut(s) 147, 532
BseLI CCNNNNNNNGG 1 cut(s) 438
BseMII CTCAG 3 cut(s) 477, 737, 822
BseX3I CGGCCG 1 cut(s) 60
BseXI GCAGC 1 cut(s) 829
BsgI GTGCAG 1 cut(s) 326
Bsh1236I CGCG 1 cut(s) 585
Bsh1285I CGRYCG 1 cut(s) 63
BshFI GGCC 2 cut(s) 62, 578
BsiEI CGRYCG 1 cut(s) 63
BsiHKAI GWGCWC 1 cut(s) 839
BsiSI CCGG 1 cut(s) 63
BslFI GGGAC 2 cut(s) 130, 682
BslI CCNNNNNNNGG 1 cut(s) 438
BsmAI GTCTC 2 cut(s) 321, 520
BsmFI GGGAC 2 cut(s) 130, 682
BsmI GAATGC 1 cut(s) 568
BsnI GGCC 2 cut(s) 62, 578
Bso31I GGTCTC 1 cut(s) 321
Bsp1286I GDGCHC 1 cut(s) 839
Bsp143I GATC 1 cut(s) 403
BspACI CCGC 3 cut(s) 585, 588, 815
BspANI GGCC 2 cut(s) 62, 578
BspCNI CTCAG 3 cut(s) 476, 736, 823
BspFNI CGCG 1 cut(s) 585
BspHI TCATGA 1 cut(s) 286
BspLI GGNNCC 1 cut(s) 443
BspMAI CTGCAG 1 cut(s) 797
BspPI GGATC 1 cut(s) 398
BspTNI GGTCTC 1 cut(s) 321
BssECI CCNNGG 2 cut(s) 147, 532
BssMI GATC 1 cut(s) 403
BssNI GRCGYC 1 cut(s) 56
BssT1I CCWWGG 1 cut(s) 532
Bst2UI CCWGG 2 cut(s) 149, 231
Bst4CI ACNGT 2 cut(s) 250, 387
BstACI GRCGYC 1 cut(s) 56
BstC8I GCNNGC 2 cut(s) 244, 658
BstDEI CTNAG 7 cut(s) 6, 320, 463, 499, 507, 723, 831
BstFNI CGCG 1 cut(s) 585
BstH2I RGCGCY 1 cut(s) 811
BstHHI GCGC 1 cut(s) 810
BstKTI GATC 1 cut(s) 406
BstMAI GTCTC 2 cut(s) 321, 520
BstMBI GATC 1 cut(s) 403
BstMCI CGRYCG 1 cut(s) 63
BstMWI GCNNNNNNNGC 3 cut(s) 59, 220, 814
BstNI CCWGG 2 cut(s) 149, 231
BstNSI RCATGY 1 cut(s) 541
BstSCI CCNGG 2 cut(s) 147, 229
BstSFI CTRYAG 2 cut(s) 634, 793
BstUI CGCG 1 cut(s) 585
BstV1I GCAGC 1 cut(s) 829
BstV2I GAAGAC 2 cut(s) 605, 660
BstZI CGGCCG 1 cut(s) 60
BsuRI GGCC 2 cut(s) 62, 578
BtgZI GCGATG 1 cut(s) 114
Cac8I GCNNGC 2 cut(s) 244, 658
CciI TCATGA 1 cut(s) 286
CfoI GCGC 1 cut(s) 810
Cfr13I GGNCC 2 cut(s) 529, 576
Csp6I GTAC 2 cut(s) 26, 190
CspCI CAANNNNNGTGG 4 cut(s) 304, 339, 521, 556
CviAII CATG 4 cut(s) 93, 287, 538, 778
CviJI RGCY 9 cut(s) 62, 223, 257, 555, 578, 617, 629, 656, 714
CviKI_1 RGCY 9 cut(s) 62, 223, 257, 555, 578, 617, 629, 656, 714
CviQI GTAC 2 cut(s) 26, 190
DdeI CTNAG 7 cut(s) 6, 320, 463, 499, 507, 723, 831
DpnI GATC 1 cut(s) 405
DpnII GATC 1 cut(s) 403
DrdI GACNNNNNNGTC 1 cut(s) 163
DseDI GACNNNNNNGTC 1 cut(s) 163
EaeI YGGCCR 1 cut(s) 60
EagI CGGCCG 1 cut(s) 60
EciI GGCGGA 1 cut(s) 603
EclXI CGGCCG 1 cut(s) 60
Eco130I CCWWGG 1 cut(s) 532
Eco31I GGTCTC 1 cut(s) 321
Eco47I GGWCC 1 cut(s) 529
Eco47III AGCGCT 1 cut(s) 809
Eco52I CGGCCG 1 cut(s) 60
Eco57I CTGAAG 1 cut(s) 654
EcoRII CCWGG 2 cut(s) 147, 229
EcoT14I CCWWGG 1 cut(s) 532
ErhI CCWWGG 1 cut(s) 532
FaeI CATG 4 cut(s) 96, 290, 541, 781
FaqI GGGAC 2 cut(s) 130, 682
FatI CATG 4 cut(s) 92, 286, 537, 777
FauI CCCGC 1 cut(s) 822
Fnu4HI GCNGC 2 cut(s) 586, 843
Fsp4HI GCNGC 2 cut(s) 586, 843
FspBI CTAG 2 cut(s) 23, 311
GlaI GCGC 1 cut(s) 809
GluI GCNGC 2 cut(s) 586, 843
HaeII RGCGCY 1 cut(s) 811
HaeIII GGCC 2 cut(s) 62, 578
HapII CCGG 1 cut(s) 63
HhaI GCGC 1 cut(s) 810
Hin1I GRCGYC 1 cut(s) 56
Hin1II CATG 4 cut(s) 96, 290, 541, 781
Hin6I GCGC 1 cut(s) 808
HinP1I GCGC 1 cut(s) 808
HincII GTYRAC 1 cut(s) 720
HindII GTYRAC 1 cut(s) 720
HindIII AAGCTT 2 cut(s) 221, 654
HinfI GANTC 7 cut(s) 133, 184, 503, 513, 707, 734, 753
HpaI GTTAAC 1 cut(s) 720
HpaII CCGG 1 cut(s) 63
Hpy166II GTNNAC 1 cut(s) 720
Hpy188I TCNGA 2 cut(s) 200, 768
Hpy188III TCNNGA 3 cut(s) 287, 493, 830
Hpy8I GTNNAC 1 cut(s) 720
Hpy99I CGWCG 2 cut(s) 58, 61
HpyAV CCTTC 3 cut(s) 361, 631, 699
HpyCH4III ACNGT 2 cut(s) 250, 387
HpyCH4IV ACGT 1 cut(s) 56
HpyCH4V TGCA 5 cut(s) 214, 343, 566, 660, 795
HpyF10VI GCNNNNNNNGC 3 cut(s) 59, 220, 814
HpyF3I CTNAG 7 cut(s) 6, 320, 463, 499, 507, 723, 831
HpySE526I ACGT 1 cut(s) 56
Hsp92I GRCGYC 1 cut(s) 56
Hsp92II CATG 4 cut(s) 96, 290, 541, 781
HspAI GCGC 1 cut(s) 808
KspAI GTTAAC 1 cut(s) 720
Kzo9I GATC 1 cut(s) 403
LmnI GCTCC 2 cut(s) 379, 609
Lsp1109I GCAGC 1 cut(s) 829
MaeI CTAG 2 cut(s) 23, 311
MaeII ACGT 1 cut(s) 56
MaeIII GTNAC 3 cut(s) 233, 250, 726
MalI GATC 1 cut(s) 405
MboI GATC 1 cut(s) 403
MboII GAAGA 5 cut(s) 193, 451, 610, 660, 781
MhlI GDGCHC 1 cut(s) 839
MluCI AATT 1 cut(s) 204
MlyI GAGTC 3 cut(s) 142, 522, 716
MnlI CCTC 3 cut(s) 456, 640, 837
MseI TTAA 1 cut(s) 719
MspI CCGG 1 cut(s) 63
MspR9I CCNGG 2 cut(s) 149, 231
Mva1269I GAATGC 1 cut(s) 568
MvaI CCWGG 2 cut(s) 149, 231
MvnI CGCG 1 cut(s) 585
MwoI GCNNNNNNNGC 3 cut(s) 59, 220, 814
NdeII GATC 1 cut(s) 403
NlaIII CATG 4 cut(s) 96, 290, 541, 781
NlaIV GGNNCC 1 cut(s) 443
NmuCI GTSAC 1 cut(s) 726
NspI RCATGY 1 cut(s) 541
PagI TCATGA 1 cut(s) 286
PctI GAATGC 1 cut(s) 568
PfeI GAWTC 4 cut(s) 184, 503, 734, 753
PflMI CCANNNNNTGG 1 cut(s) 438
PkrI GCNGC 2 cut(s) 587, 844
PleI GAGTC 3 cut(s) 141, 521, 715
PpsI GAGTC 3 cut(s) 141, 521, 715
Psp6I CCWGG 2 cut(s) 147, 229
PspGI CCWGG 2 cut(s) 147, 229
PspN4I GGNNCC 1 cut(s) 443
PspPI GGNCC 2 cut(s) 529, 576
PstI CTGCAG 1 cut(s) 797
RsaI GTAC 2 cut(s) 27, 191
RsaNI GTAC 2 cut(s) 26, 190
SaqAI TTAA 1 cut(s) 719
SatI GCNGC 2 cut(s) 586, 843
Sau3AI GATC 1 cut(s) 403
Sau96I GGNCC 2 cut(s) 529, 576
ScaI AGTACT 2 cut(s) 27, 191
SchI GAGTC 3 cut(s) 142, 522, 716
ScrFI CCNGG 2 cut(s) 149, 231
SduI GDGCHC 1 cut(s) 839
SetI ASST 8 cut(s) 59, 225, 259, 321, 396, 428, 658, 716
SfcI CTRYAG 2 cut(s) 634, 793
SinI GGWCC 1 cut(s) 529
SmlI CTYRAG 1 cut(s) 550
SmoI CTYRAG 1 cut(s) 550
Sse9I AATT 1 cut(s) 204
SsiI CCGC 3 cut(s) 585, 588, 815
SspI AATATT 1 cut(s) 352
SspMI CTAG 2 cut(s) 23, 311
StyD4I CCNGG 2 cut(s) 147, 229
StyI CCWWGG 1 cut(s) 532
TaaI ACNGT 2 cut(s) 250, 387
TaiI ACGT 1 cut(s) 59
TaqI TCGA 5 cut(s) 120, 179, 303, 710, 732
TasI AATT 1 cut(s) 204
TatI WGTACW 2 cut(s) 25, 189
TauI GCSGC 1 cut(s) 588
TfiI GAWTC 4 cut(s) 184, 503, 734, 753
Tru1I TTAA 1 cut(s) 719
Tru9I TTAA 1 cut(s) 719
TseFI GTSAC 1 cut(s) 726
TseI GCWGC 1 cut(s) 842
Tsp45I GTSAC 1 cut(s) 726
TspGWI ACGGA 1 cut(s) 617
Van91I CCANNNNNTGG 1 cut(s) 438
VpaK11BI GGWCC 1 cut(s) 529
XceI RCATGY 1 cut(s) 541
XspI CTAG 2 cut(s) 23, 311
ZraI GACGTC 1 cut(s) 57
ZrmI AGTACT 2 cut(s) 27, 191
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.