FvH4_3g00840

Catalyzes xyloglucan endohydrolysis (XEH) and or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0000460
Physical Location & Seq
Forward (+)
433982 .. 435577
1596 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g00840.t1

Sequence Viewer

Length: 930 bp
ATGCCAACTTCTCTCATTCATAATCATCCTTCCATTTCCATAGTCGTACTTACTAATTTTCACATGTCTTCTTCAACTTCAATGCTTCTGTTTGTACCATTTCTGCTTGCTAGCTTTGTTATGGTTGCCTCTGCGGGTAACTTCAACCAAGACTTTAAGATTACATGGGGGGACGGTCGAGCAAATATACTCAACGACGGCCAACTTCTTACTCTCTCCCTCGACAAAGCCTCTGGCTCTGGTTTTGAATCCACAAACGAATATCTCTTCGGCAAGATTGACATGCAGCTCAAGCTTGTTGCCGGAAACTCTGCTGGCACCGTCACTGCCTACTATTTAAAATCAGAAGGGTCGACATGGGACGAGATAGACTTTGAGTTCTTGGGAAACCTGAGTGGCAACCCTTACATTCTTCACACCAATGTGTTCAGCCAAGGCAAAGGTAACAGAGAGCAGCAGTTCCATCTCTGGTTCGACCCAACTGCTGATTTCCACACCTATTCCATTCTCTGGAATCCACAGCGCATTGTTTTCTCTGTTGATGGAACCCCCATTAGAGAGTTCAAGAACCAAGAATCAAATGGTGTTCCATTCCCAAAGAGCCAAGCAATGAGAATACACTCGAGCCTTTGGAATGCCGACGATTGGGCGACAATGGGTGGACGCGTGAAGACGGATTGGAATGCAGCTCCTTTCACTGCCTCTTACAGGAATTTCAATGCTGAAGCTTGCATTTGGGCGTCTGGATCATCTTCTTGCGGCTCAAGCAATGCCCCTTCTTCCACTAATGGCGATTGGGTTTCGCAAGAATTGGACAGTGCAAGTCAAAAGAAGATGAGTTGGGTGCAGAAAAACTACATGATCTACAATTACTGCACAGATGTCAACAGATTCCCTCAGGGTCTCCCAGTTGAATGCAGCGCTTCATAG

Protein Analysis

310

Amino Acids

34.3

Weight (kDa)

5.45

Isoelectric Point (pI)

36.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_16 PF00722 48 - 228 3e-67 Glycosyl hydrolases family 16
XET_C PF06955 262 - 306 7.6e-21 Xyloglucan endo-transglycosylase (XET) C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000460)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G25810 AT4G30270 AT5G57550 AT5G57560
fragaria_vesca FvH4_3g00833 FvH4_3g00840 FvH4_3g00850 FvH4_4g06700 FvH4_4g09230
malus_domestica MD13G1237300.v1.1 MD13G1268000.v1.1 MD13G1268900.v1.1 MD16G1267200.v1.1 MD16G1267300.v1.1
prunus_persica Prupe.1G069800_v2.0.a1 Prupe.1G088600_v2.0.a1 Prupe.1G088800_v2.0.a1 Prupe.1G088900_v2.0.a1 Prupe.1G089000_v2.0.a1
pyrus_communis pycom13g21020 pycom13g22350 pycom13g22380 pycom16g23630 pycom16g23640
rosa_chinensis RchiOBHm_Chr4g0398631 RchiOBHm_Chr4g0402891 RchiOBHm_Chr4g0402911 RchiOBHm_Chr4g0402921 RchiOBHm_Chr4g0402931 RchiOBHm_Chr4g0402951 RchiOBHm_Chr4g0402961 RchiOBHm_Chr4g0402981
rosa_laevigata RLG00000009031 RLG00000009033 RLG00000009034 RLG00000009035 RLG00000009039 RLG00000009040 RLG00000009339
rosa_multiflora Rmu_co8186292.1_g000001 Rmu_sc0000990.1_g000004 Rmu_sc0001023.1_g000002 Rmu_sc0001023.1_g000004 Rmu_sc0001023.1_g000007 Rmu_sc0001023.1_g000015 Rmu_sc0002178.1_g000007 Rmu_sc0002451.1_g000069 Rmu_sc0002451.1_g000079 Rmu_sc0002451.1_g000080 Rmu_sc0008058.1_g000026 Rmu_sc0008058.1_g000029 Rmu_sc0008058.1_g000030
rosa_roxburghii Rroxscaffold_5G00343590 Rroxscaffold_5G00347620 Rroxscaffold_5G00347630 Rroxscaffold_5G00347640 Rroxscaffold_5G00347650 Rroxscaffold_5G00347660 Rroxscaffold_5G00347670 Rroxscaffold_5G00347690
rosa_rugosa Rorug04G0009900 Rorug04G0039000 Rorug04G0039100 Rorug04G0039200 Rorug04G0039300 Rorug04G0039400 Rorug04G0039500 Rorug04G0039600 Rorug04G0039600 Rorug04G0040200
rosa_samantha Rh4AG087800 Rh4AG116500 Rh4AG116700 Rh4AG116800 Rh4AG116900 Rh4AG117000 Rh4AG117100 Rh4BG109300 Rh4BG109400 Rh4BG109500 Rh4BG109600 Rh4BG109700 Rh4BG109800 Rh4CG096100 Rh4CG123700 Rh4CG124200
rosa_wichuraiana Rw4G007280 Rw4G009320 Rw4G009340 Rw4G009370 Rw4G009380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 317
AccB7I CCANNNNNTGG 1 cut(s) 510
AccI GTMKAC 1 cut(s) 353
AccII CGCG 1 cut(s) 666
AciI CCGC 2 cut(s) 134, 759
AclWI GGATC 1 cut(s) 754
AcoI YGGCCR 1 cut(s) 199
AcsI RAATTY 1 cut(s) 712
AcuI CTGAAG 1 cut(s) 744
AcyI GRCGYC 1 cut(s) 740
AfaI GTAC 2 cut(s) 48, 96
AfeI AGCGCT 1 cut(s) 922
AfiI CCNNNNNNNGG 3 cut(s) 510, 645, 708
AflIII ACRYGT 2 cut(s) 63, 664
AgsI TTSAA 7 cut(s) 75, 81, 145, 248, 565, 718, 914
AleI CACNNNNGTG 1 cut(s) 422
AluBI AGCT 5 cut(s) 114, 289, 295, 689, 728
AluI AGCT 5 cut(s) 114, 289, 295, 689, 728
Alw26I GTCTC 1 cut(s) 908
AlwI GGATC 1 cut(s) 754
Ama87I CYCGRG 1 cut(s) 622
Aor51HI AGCGCT 1 cut(s) 922
AoxI GGCC 1 cut(s) 199
ApeKI GCWGC 4 cut(s) 286, 454, 686, 918
ApoI RAATTY 1 cut(s) 712
AspLEI GCGC 2 cut(s) 525, 923
AsuNHI GCTAGC 1 cut(s) 110
AvaI CYCGRG 1 cut(s) 622
AxyI CCTNAGG 1 cut(s) 897
BanI GGYRCC 1 cut(s) 317
BbsI GAAGAC 2 cut(s) 60, 677
BbvI GCAGC 3 cut(s) 298, 466, 698
BccI CCATC 2 cut(s) 471, 536
BceAI ACGGC 1 cut(s) 214
BcgI CGANNNNNNTGC 2 cut(s) 464, 498
BcoDI GTCTC 1 cut(s) 908
BfaI CTAG 1 cut(s) 111
BfoI RGCGCY 1 cut(s) 924
BisI GCNGC 5 cut(s) 287, 455, 687, 760, 919
BlsI GCNGC 5 cut(s) 288, 456, 688, 761, 920
BmeT110I CYCGRG 1 cut(s) 622
BmiI GGNNCC 2 cut(s) 319, 547
BmrI ACTGGG 1 cut(s) 902
BmtI GCTAGC 1 cut(s) 114
BmuI ACTGGG 1 cut(s) 902
BpiI GAAGAC 2 cut(s) 60, 677
BpuEI CTTGAG 2 cut(s) 275, 748
BsaHI GRCGYC 1 cut(s) 740
BsaI GGTCTC 1 cut(s) 908
BsaJI CCNNGG 1 cut(s) 433
Bsc4I CCNNNNNNNGG 3 cut(s) 510, 645, 708
Bse1I ACTGG 1 cut(s) 908
Bse21I CCTNAGG 1 cut(s) 897
Bse3DI GCAATG 2 cut(s) 615, 775
BseDI CCNNGG 1 cut(s) 433
BseGI GGATG 1 cut(s) 25
BseLI CCNNNNNNNGG 3 cut(s) 510, 645, 708
BseMI GCAATG 2 cut(s) 615, 775
BseMII CTCAG 2 cut(s) 383, 911
BseNI ACTGG 1 cut(s) 908
BseXI GCAGC 3 cut(s) 298, 466, 698
BsgI GTGCAG 2 cut(s) 859, 866
Bsh1236I CGCG 1 cut(s) 666
Bsh1285I CGRYCG 1 cut(s) 178
BshFI GGCC 1 cut(s) 201
BshNI GGYRCC 1 cut(s) 317
BsiEI CGRYCG 1 cut(s) 178
BsiHKCI CYCGRG 1 cut(s) 622
BsiSI CCGG 1 cut(s) 303
BslFI GGGAC 2 cut(s) 185, 374
BslI CCNNNNNNNGG 3 cut(s) 510, 645, 708
BsmAI GTCTC 1 cut(s) 908
BsmFI GGGAC 2 cut(s) 185, 374
BsmI GAATGC 3 cut(s) 640, 688, 920
BsnI GGCC 1 cut(s) 201
Bso31I GGTCTC 1 cut(s) 908
BsoBI CYCGRG 1 cut(s) 622
Bsp143I GATC 2 cut(s) 746, 861
BspACI CCGC 2 cut(s) 134, 759
BspANI GGCC 1 cut(s) 201
BspCNI CTCAG 2 cut(s) 384, 910
BspFNI CGCG 1 cut(s) 666
BspLI GGNNCC 2 cut(s) 319, 547
BspOI GCTAGC 1 cut(s) 114
BspPI GGATC 1 cut(s) 754
BspT107I GGYRCC 1 cut(s) 317
BspTNI GGTCTC 1 cut(s) 908
BsrDI GCAATG 2 cut(s) 615, 775
BsrI ACTGG 1 cut(s) 908
BssECI CCNNGG 1 cut(s) 433
BssMI GATC 2 cut(s) 746, 861
BssNI GRCGYC 1 cut(s) 740
BssT1I CCWWGG 1 cut(s) 433
Bst4CI ACNGT 3 cut(s) 176, 322, 818
Bst6I CTCTTC 1 cut(s) 272
BstACI GRCGYC 1 cut(s) 740
BstC8I GCNNGC 4 cut(s) 108, 112, 316, 730
BstDEI CTNAG 2 cut(s) 392, 897
BstENI CCTNNNNNAGG 1 cut(s) 706
BstF5I GGATG 1 cut(s) 25
BstFNI CGCG 1 cut(s) 666
BstH2I RGCGCY 1 cut(s) 924
BstHHI GCGC 2 cut(s) 525, 923
BstKTI GATC 2 cut(s) 749, 864
BstMAI GTCTC 1 cut(s) 908
BstMBI GATC 2 cut(s) 746, 861
BstMCI CGRYCG 1 cut(s) 178
BstMWI GCNNNNNNNGC 2 cut(s) 292, 765
BstNSI RCATGY 2 cut(s) 67, 286
BstUI CGCG 1 cut(s) 666
BstV1I GCAGC 3 cut(s) 298, 466, 698
BstV2I GAAGAC 2 cut(s) 60, 677
Bsu36I CCTNAGG 1 cut(s) 897
BsuRI GGCC 1 cut(s) 201
BtsCI GGATG 1 cut(s) 25
BtsI GCAGTG 2 cut(s) 324, 696
BtsIMutI CAGTG 3 cut(s) 324, 696, 823
Cac8I GCNNGC 4 cut(s) 108, 112, 316, 730
CfoI GCGC 2 cut(s) 525, 923
CseI GACGC 2 cut(s) 672, 729
Csp6I GTAC 2 cut(s) 47, 95
CviAII CATG 5 cut(s) 64, 165, 283, 357, 859
CviQI GTAC 2 cut(s) 47, 95
DdeI CTNAG 2 cut(s) 392, 897
DpnI GATC 2 cut(s) 748, 863
DpnII GATC 2 cut(s) 746, 861
DraI TTTAAA 1 cut(s) 339
EaeI YGGCCR 1 cut(s) 199
Eam1104I CTCTTC 1 cut(s) 272
EarI CTCTTC 1 cut(s) 272
Eco130I CCWWGG 1 cut(s) 433
Eco31I GGTCTC 1 cut(s) 908
Eco47III AGCGCT 1 cut(s) 922
Eco57I CTGAAG 1 cut(s) 744
Eco81I CCTNAGG 1 cut(s) 897
Eco88I CYCGRG 1 cut(s) 622
EcoNI CCTNNNNNAGG 1 cut(s) 706
EcoT14I CCWWGG 1 cut(s) 433
ErhI CCWWGG 1 cut(s) 433
FaeI CATG 5 cut(s) 67, 168, 286, 360, 862
FaqI GGGAC 2 cut(s) 185, 374
FatI CATG 5 cut(s) 63, 164, 282, 356, 858
FauI CCCGC 1 cut(s) 127
FblI GTMKAC 1 cut(s) 353
Fnu4HI GCNGC 5 cut(s) 287, 455, 687, 760, 919
FokI GGATG 1 cut(s) 12
Fsp4HI GCNGC 5 cut(s) 287, 455, 687, 760, 919
FspBI CTAG 1 cut(s) 111
GlaI GCGC 2 cut(s) 524, 922
GluI GCNGC 5 cut(s) 287, 455, 687, 760, 919
HaeII RGCGCY 1 cut(s) 924
HaeIII GGCC 1 cut(s) 201
HapII CCGG 1 cut(s) 303
HgaI GACGC 2 cut(s) 672, 729
HhaI GCGC 2 cut(s) 525, 923
Hin1I GRCGYC 1 cut(s) 740
Hin1II CATG 5 cut(s) 67, 168, 286, 360, 862
Hin6I GCGC 2 cut(s) 523, 921
HinP1I GCGC 2 cut(s) 523, 921
HincII GTYRAC 2 cut(s) 354, 886
HindII GTYRAC 2 cut(s) 354, 886
HindIII AAGCTT 2 cut(s) 293, 726
HinfI GANTC 4 cut(s) 248, 514, 575, 891
HpaII CCGG 1 cut(s) 303
Hpy166II GTNNAC 3 cut(s) 354, 662, 886
Hpy188I TCNGA 1 cut(s) 346
Hpy188III TCNNGA 3 cut(s) 511, 565, 744
Hpy8I GTNNAC 3 cut(s) 354, 662, 886
Hpy99I CGWCG 2 cut(s) 200, 644
HpyAV CCTTC 3 cut(s) 39, 341, 786
HpyCH4III ACNGT 3 cut(s) 176, 322, 818
HpyCH4V TGCA 7 cut(s) 286, 686, 732, 821, 847, 876, 918
HpyF10VI GCNNNNNNNGC 2 cut(s) 292, 765
HpyF3I CTNAG 2 cut(s) 392, 897
Hsp92I GRCGYC 1 cut(s) 740
Hsp92II CATG 5 cut(s) 67, 168, 286, 360, 862
HspAI GCGC 2 cut(s) 523, 921
Kzo9I GATC 2 cut(s) 746, 861
LmnI GCTCC 1 cut(s) 694
Lsp1109I GCAGC 3 cut(s) 298, 466, 698
MaeI CTAG 1 cut(s) 111
MaeIII GTNAC 3 cut(s) 137, 322, 443
MalI GATC 2 cut(s) 748, 863
MboI GATC 2 cut(s) 746, 861
MboII GAAGA 8 cut(s) 60, 63, 259, 404, 682, 744, 771, 844
MluCI AATT 4 cut(s) 55, 712, 809, 868
MluI ACGCGT 1 cut(s) 664
MnlI CCTC 5 cut(s) 139, 230, 241, 712, 906
MseI TTAA 2 cut(s) 156, 338
MslI CAYNNNNRTG 2 cut(s) 420, 422
MspI CCGG 1 cut(s) 303
Mva1269I GAATGC 3 cut(s) 640, 688, 920
MvnI CGCG 1 cut(s) 666
MwoI GCNNNNNNNGC 2 cut(s) 292, 765
NdeII GATC 2 cut(s) 746, 861
NheI GCTAGC 1 cut(s) 110
NlaIII CATG 5 cut(s) 67, 168, 286, 360, 862
NlaIV GGNNCC 2 cut(s) 319, 547
NmuCI GTSAC 1 cut(s) 322
NspI RCATGY 2 cut(s) 67, 286
OliI CACNNNNGTG 1 cut(s) 422
PaeR7I CTCGAG 1 cut(s) 622
PciI ACATGT 1 cut(s) 63
PctI GAATGC 3 cut(s) 640, 688, 920
PfeI GAWTC 4 cut(s) 248, 514, 575, 891
PflMI CCANNNNNTGG 1 cut(s) 510
PkrI GCNGC 5 cut(s) 288, 456, 688, 761, 920
PscI ACATGT 1 cut(s) 63
PspN4I GGNNCC 2 cut(s) 319, 547
PspXI VCTCGAGB 1 cut(s) 622
RsaI GTAC 2 cut(s) 48, 96
RsaNI GTAC 2 cut(s) 47, 95
RseI CAYNNNNRTG 2 cut(s) 420, 422
SalI GTCGAC 1 cut(s) 352
SaqAI TTAA 2 cut(s) 156, 338
SatI GCNGC 5 cut(s) 287, 455, 687, 760, 919
Sau3AI GATC 2 cut(s) 746, 861
SetI ASST 8 cut(s) 116, 291, 297, 393, 445, 500, 691, 730
Sfr274I CTCGAG 1 cut(s) 622
SlaI CTCGAG 1 cut(s) 622
SmiMI CAYNNNNRTG 2 cut(s) 420, 422
SmlI CTYRAG 3 cut(s) 290, 622, 763
SmoI CTYRAG 3 cut(s) 290, 622, 763
Sse9I AATT 4 cut(s) 55, 712, 809, 868
SsiI CCGC 2 cut(s) 134, 759
SspMI CTAG 1 cut(s) 111
StyI CCWWGG 1 cut(s) 433
TaaI ACNGT 3 cut(s) 176, 322, 818
TaqI TCGA 5 cut(s) 178, 222, 353, 474, 623
TasI AATT 4 cut(s) 55, 712, 809, 868
TauI GCSGC 1 cut(s) 762
TfiI GAWTC 4 cut(s) 248, 514, 575, 891
Tru1I TTAA 2 cut(s) 156, 338
Tru9I TTAA 2 cut(s) 156, 338
TscAI CASTG 3 cut(s) 331, 703, 823
TseFI GTSAC 1 cut(s) 322
TseI GCWGC 4 cut(s) 286, 454, 686, 918
Tsp45I GTSAC 1 cut(s) 322
TspDTI ATGAA 2 cut(s) 8, 915
TspGWI ACGGA 1 cut(s) 689
TspRI CASTG 3 cut(s) 331, 703, 823
Van91I CCANNNNNTGG 1 cut(s) 510
XagI CCTNNNNNAGG 1 cut(s) 706
XapI RAATTY 1 cut(s) 712
XceI RCATGY 2 cut(s) 67, 286
XcmI CCANNNNNNNNNTGG 1 cut(s) 578
XhoI CTCGAG 1 cut(s) 622
XmiI GTMKAC 1 cut(s) 353
XspI CTAG 1 cut(s) 111
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.