Rroxscaffold_5G00347660

Catalyzes xyloglucan endohydrolysis (XEH) and or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0000460
Physical Location & Seq
Forward (+)
19246177 .. 19247537
1361 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00347660.1

Sequence Viewer

Length: 849 bp
ATGCTTCTGTTCGTTCCATTTCTGCTGATTAGCTCTGTTATGGTTGCCTCAGCTGGTAACTTCAACCAAGACTTTCAGGTTACATGGGGGGACGGTCGAGCCAACATAGTCAACGACGGCCAACTTCTTACTCTCTCCCTCGACAAAGCCTCTGGCTCTGGTTTTGAATCCAAAAACGAATACCTCTTCGGCAAGATTGACATGCAGCTCAAGCTTGTCGCCGGAAACTCTGCTGGCACCGTCACCGCCTACTATTTAAAATCAGAAGGGTCTGCATGGGATGAGATAGACTTTGAGTTCTTGGGAAACCTGACCGGCAACCCTTACACTCTTCACACCAATGTCTTCAGCCAAGGCAAAGGAAACAGAGAACAGCAATTCCATCTCTGGTTCGACCCAACTGCTGATTTCCACACCTATTCCATTCTCTGGAATCCACAGCGCATTGTTTTCTCTGTTGATGGTACTCCCATTAGAGAGTTCAAGAACCAAGAGTGGAAAGGTGTTCCATTCCCAAAGAGCCAAGCAATGAGAATACACTCGAGCCTCTGGAATGCTGATGATTGGGCAACAATGGGAGGACGTGTAAAGACAGATTGGAATGCAGCTCCGTTCACTGCCTCTTACAGGAATTTCAATGCTGAAGCATGCATTTGGGCTTCTGGATCATCTTCTTGCGGTTCAAGCAGTGCCCCTGCTTCGACTAATGGAGATTGGCTTTCGCAAGAATTGGACACTGCTAGTTATGAAAAGCTGAGTTGGGTGCAGAAAAACTACATGATCTACGATTACTGCACAGATGTCAACAGATTCCCCCAGGGTCTCCCAGTTGAATGCAGCACTTCATAG

Protein Analysis

282

Amino Acids

31.48

Weight (kDa)

4.9

Isoelectric Point (pI)

28.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_16 PF00722 21 - 201 2e-67 Glycosyl hydrolases family 16
XET_C PF06955 235 - 279 2.1e-19 Xyloglucan endo-transglycosylase (XET) C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000460)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G25810 AT4G30270 AT5G57550 AT5G57560
fragaria_vesca FvH4_3g00833 FvH4_3g00840 FvH4_3g00850 FvH4_4g06700 FvH4_4g09230
malus_domestica MD13G1237300.v1.1 MD13G1268000.v1.1 MD13G1268900.v1.1 MD16G1267200.v1.1 MD16G1267300.v1.1
prunus_persica Prupe.1G069800_v2.0.a1 Prupe.1G088600_v2.0.a1 Prupe.1G088800_v2.0.a1 Prupe.1G088900_v2.0.a1 Prupe.1G089000_v2.0.a1
pyrus_communis pycom13g21020 pycom13g22350 pycom13g22380 pycom16g23630 pycom16g23640
rosa_chinensis RchiOBHm_Chr4g0398631 RchiOBHm_Chr4g0402891 RchiOBHm_Chr4g0402911 RchiOBHm_Chr4g0402921 RchiOBHm_Chr4g0402931 RchiOBHm_Chr4g0402951 RchiOBHm_Chr4g0402961 RchiOBHm_Chr4g0402981
rosa_laevigata RLG00000009031 RLG00000009033 RLG00000009034 RLG00000009035 RLG00000009039 RLG00000009040 RLG00000009339
rosa_multiflora Rmu_co8186292.1_g000001 Rmu_sc0000990.1_g000004 Rmu_sc0001023.1_g000002 Rmu_sc0001023.1_g000004 Rmu_sc0001023.1_g000007 Rmu_sc0001023.1_g000015 Rmu_sc0002178.1_g000007 Rmu_sc0002451.1_g000069 Rmu_sc0002451.1_g000079 Rmu_sc0002451.1_g000080 Rmu_sc0008058.1_g000026 Rmu_sc0008058.1_g000029 Rmu_sc0008058.1_g000030
rosa_roxburghii Rroxscaffold_5G00343590 Rroxscaffold_5G00347620 Rroxscaffold_5G00347630 Rroxscaffold_5G00347640 Rroxscaffold_5G00347650 Rroxscaffold_5G00347660 Rroxscaffold_5G00347670 Rroxscaffold_5G00347690
rosa_rugosa Rorug04G0009900 Rorug04G0039000 Rorug04G0039100 Rorug04G0039200 Rorug04G0039300 Rorug04G0039400 Rorug04G0039500 Rorug04G0039600 Rorug04G0039600 Rorug04G0040200
rosa_samantha Rh4AG087800 Rh4AG116500 Rh4AG116700 Rh4AG116800 Rh4AG116900 Rh4AG117000 Rh4AG117100 Rh4BG109300 Rh4BG109400 Rh4BG109500 Rh4BG109600 Rh4BG109700 Rh4BG109800 Rh4CG096100 Rh4CG123700 Rh4CG124200
rosa_wichuraiana Rw4G007280 Rw4G009320 Rw4G009340 Rw4G009370 Rw4G009380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 236
AccB7I CCANNNNNTGG 1 cut(s) 429
AciI CCGC 2 cut(s) 246, 678
AclWI GGATC 1 cut(s) 673
AcoI YGGCCR 1 cut(s) 118
AcsI RAATTY 1 cut(s) 631
AcuI CTGAAG 2 cut(s) 331, 663
AfaI GTAC 1 cut(s) 466
AfiI CCNNNNNNNGG 2 cut(s) 429, 627
AflIII ACRYGT 1 cut(s) 583
AgsI TTSAA 6 cut(s) 64, 167, 484, 637, 684, 833
AjiI CACGTC 1 cut(s) 584
AjnI CCWGG 1 cut(s) 816
AloI GAACNNNNNNTCC 2 cut(s) 363, 395
AluBI AGCT 6 cut(s) 33, 53, 208, 214, 608, 754
AluI AGCT 6 cut(s) 33, 53, 208, 214, 608, 754
Alw26I GTCTC 1 cut(s) 827
AlwI GGATC 1 cut(s) 673
Ama87I CYCGRG 1 cut(s) 541
AoxI GGCC 1 cut(s) 118
ApeKI GCWGC 3 cut(s) 205, 605, 837
ApoI RAATTY 1 cut(s) 631
AspLEI GCGC 1 cut(s) 444
AsuHPI GGTGA 1 cut(s) 235
AvaI CYCGRG 1 cut(s) 541
BaeGI GKGCMC 1 cut(s) 694
BanI GGYRCC 1 cut(s) 236
BbsI GAAGAC 1 cut(s) 337
BbvCI CCTCAGC 1 cut(s) 49
BbvI GCAGC 2 cut(s) 217, 617
BccI CCATC 2 cut(s) 390, 455
BceAI ACGGC 1 cut(s) 133
BcgI CGANNNNNNTGC 2 cut(s) 383, 417
BciT130I CCWGG 1 cut(s) 818
BcoDI GTCTC 1 cut(s) 827
BfaI CTAG 1 cut(s) 741
BisI GCNGC 3 cut(s) 206, 606, 838
BlsI GCNGC 3 cut(s) 207, 607, 839
Bme1390I CCNGG 1 cut(s) 818
BmeT110I CYCGRG 1 cut(s) 541
BmgBI CACGTC 1 cut(s) 584
BmiI GGNNCC 1 cut(s) 238
BmrFI CCNGG 1 cut(s) 818
BmrI ACTGGG 1 cut(s) 821
BmuI ACTGGG 1 cut(s) 821
BpiI GAAGAC 1 cut(s) 337
Bpu10I CCTNAGC 1 cut(s) 49
BpuEI CTTGAG 1 cut(s) 194
BsaI GGTCTC 1 cut(s) 827
BsaJI CCNNGG 3 cut(s) 352, 816, 817
BsaXI ACNNNNNCTCC 2 cut(s) 570, 600
Bsc4I CCNNNNNNNGG 2 cut(s) 429, 627
Bse118I RCCGGY 1 cut(s) 314
Bse1I ACTGG 1 cut(s) 827
Bse3DI GCAATG 1 cut(s) 534
BseBI CCWGG 1 cut(s) 818
BseDI CCNNGG 3 cut(s) 352, 816, 817
BseGI GGATG 1 cut(s) 286
BseLI CCNNNNNNNGG 2 cut(s) 429, 627
BseMI GCAATG 1 cut(s) 534
BseMII CTCAG 2 cut(s) 63, 746
BseNI ACTGG 1 cut(s) 827
BseSI GKGCMC 1 cut(s) 694
BseXI GCAGC 2 cut(s) 217, 617
BsgI GTGCAG 2 cut(s) 778, 785
Bsh1285I CGRYCG 1 cut(s) 97
BshFI GGCC 1 cut(s) 120
BshNI GGYRCC 1 cut(s) 236
BsiEI CGRYCG 1 cut(s) 97
BsiHKCI CYCGRG 1 cut(s) 541
BsiSI CCGG 2 cut(s) 222, 315
BslFI GGGAC 1 cut(s) 104
BslI CCNNNNNNNGG 2 cut(s) 429, 627
BsmAI GTCTC 1 cut(s) 827
BsmFI GGGAC 1 cut(s) 104
BsmI GAATGC 3 cut(s) 559, 607, 839
BsnI GGCC 1 cut(s) 120
Bso31I GGTCTC 1 cut(s) 827
BsoBI CYCGRG 1 cut(s) 541
Bsp1286I GDGCHC 1 cut(s) 694
Bsp143I GATC 2 cut(s) 665, 780
BspACI CCGC 2 cut(s) 246, 678
BspANI GGCC 1 cut(s) 120
BspCNI CTCAG 2 cut(s) 62, 747
BspLI GGNNCC 1 cut(s) 238
BspPI GGATC 1 cut(s) 673
BspT107I GGYRCC 1 cut(s) 236
BspTNI GGTCTC 1 cut(s) 827
BsrDI GCAATG 1 cut(s) 534
BsrFI RCCGGY 1 cut(s) 314
BsrI ACTGG 1 cut(s) 827
BssAI RCCGGY 1 cut(s) 314
BssECI CCNNGG 3 cut(s) 352, 816, 817
BssMI GATC 2 cut(s) 665, 780
BssT1I CCWWGG 1 cut(s) 352
Bst2UI CCWGG 1 cut(s) 818
Bst4CI ACNGT 2 cut(s) 95, 241
Bst6I CTCTTC 2 cut(s) 191, 336
BstC8I GCNNGC 2 cut(s) 235, 649
BstDEI CTNAG 2 cut(s) 49, 755
BstENI CCTNNNNNAGG 1 cut(s) 625
BstF5I GGATG 1 cut(s) 286
BstHHI GCGC 1 cut(s) 444
BstKTI GATC 2 cut(s) 668, 783
BstMAI GTCTC 1 cut(s) 827
BstMBI GATC 2 cut(s) 665, 780
BstMCI CGRYCG 1 cut(s) 97
BstMWI GCNNNNNNNGC 2 cut(s) 211, 684
BstNI CCWGG 1 cut(s) 818
BstNSI RCATGY 2 cut(s) 205, 651
BstSCI CCNGG 1 cut(s) 816
BstSLI GKGCMC 1 cut(s) 694
BstV1I GCAGC 2 cut(s) 217, 617
BstV2I GAAGAC 1 cut(s) 337
BsuRI GGCC 1 cut(s) 120
BtrI CACGTC 1 cut(s) 584
BtsCI GGATG 1 cut(s) 286
BtsI GCAGTG 3 cut(s) 615, 694, 735
BtsIMutI CAGTG 3 cut(s) 615, 694, 735
Cac8I GCNNGC 2 cut(s) 235, 649
CfoI GCGC 1 cut(s) 444
Cfr10I RCCGGY 1 cut(s) 314
Csp6I GTAC 1 cut(s) 465
CviAII CATG 5 cut(s) 84, 202, 276, 648, 778
CviQI GTAC 1 cut(s) 465
DdeI CTNAG 2 cut(s) 49, 755
DpnI GATC 2 cut(s) 667, 782
DpnII GATC 2 cut(s) 665, 780
DraI TTTAAA 1 cut(s) 258
EaeI YGGCCR 1 cut(s) 118
Eam1104I CTCTTC 2 cut(s) 191, 336
EarI CTCTTC 2 cut(s) 191, 336
Eco130I CCWWGG 1 cut(s) 352
Eco31I GGTCTC 1 cut(s) 827
Eco57I CTGAAG 2 cut(s) 331, 663
Eco88I CYCGRG 1 cut(s) 541
EcoNI CCTNNNNNAGG 1 cut(s) 625
EcoRII CCWGG 1 cut(s) 816
EcoT14I CCWWGG 1 cut(s) 352
EcoT22I ATGCAT 1 cut(s) 653
ErhI CCWWGG 1 cut(s) 352
FaeI CATG 5 cut(s) 87, 205, 279, 651, 781
FaiI YATR 9 cut(s) 41, 85, 107, 203, 277, 649, 747, 779, 847
FaqI GGGAC 1 cut(s) 104
FatI CATG 5 cut(s) 83, 201, 275, 647, 777
Fnu4HI GCNGC 3 cut(s) 206, 606, 838
FokI GGATG 1 cut(s) 293
Fsp4HI GCNGC 3 cut(s) 206, 606, 838
FspBI CTAG 1 cut(s) 741
GlaI GCGC 1 cut(s) 443
GluI GCNGC 3 cut(s) 206, 606, 838
HaeIII GGCC 1 cut(s) 120
HapII CCGG 2 cut(s) 222, 315
HhaI GCGC 1 cut(s) 444
Hin1II CATG 5 cut(s) 87, 205, 279, 651, 781
Hin6I GCGC 1 cut(s) 442
HinP1I GCGC 1 cut(s) 442
HincII GTYRAC 2 cut(s) 112, 805
HindII GTYRAC 2 cut(s) 112, 805
HindIII AAGCTT 1 cut(s) 212
HinfI GANTC 3 cut(s) 167, 433, 810
HpaII CCGG 2 cut(s) 222, 315
HphI GGTGA 1 cut(s) 235
Hpy166II GTNNAC 3 cut(s) 112, 615, 805
Hpy188I TCNGA 1 cut(s) 265
Hpy188III TCNNGA 4 cut(s) 430, 484, 550, 663
Hpy8I GTNNAC 3 cut(s) 112, 615, 805
Hpy99I CGWCG 1 cut(s) 119
HpyAV CCTTC 1 cut(s) 260
HpyCH4III ACNGT 2 cut(s) 95, 241
HpyCH4IV ACGT 1 cut(s) 583
HpyCH4V TGCA 7 cut(s) 205, 275, 605, 651, 766, 795, 837
HpyF10VI GCNNNNNNNGC 2 cut(s) 211, 684
HpyF3I CTNAG 2 cut(s) 49, 755
HpySE526I ACGT 1 cut(s) 583
Hsp92II CATG 5 cut(s) 87, 205, 279, 651, 781
HspAI GCGC 1 cut(s) 442
Kzo9I GATC 2 cut(s) 665, 780
LmnI GCTCC 1 cut(s) 613
Lsp1109I GCAGC 2 cut(s) 217, 617
MaeI CTAG 1 cut(s) 741
MaeII ACGT 1 cut(s) 583
MaeIII GTNAC 3 cut(s) 56, 79, 241
MalI GATC 2 cut(s) 667, 782
MboI GATC 2 cut(s) 665, 780
MboII GAAGA 4 cut(s) 178, 323, 337, 663
MhlI GDGCHC 1 cut(s) 694
MluCI AATT 3 cut(s) 377, 631, 728
MnlI CCTC 7 cut(s) 58, 149, 160, 194, 557, 572, 631
Mph1103I ATGCAT 1 cut(s) 653
MseI TTAA 1 cut(s) 257
MslI CAYNNNNRTG 1 cut(s) 339
MspA1I CMGCKG 1 cut(s) 53
MspI CCGG 2 cut(s) 222, 315
MspR9I CCNGG 1 cut(s) 818
Mva1269I GAATGC 3 cut(s) 559, 607, 839
MvaI CCWGG 1 cut(s) 818
MwoI GCNNNNNNNGC 2 cut(s) 211, 684
NdeII GATC 2 cut(s) 665, 780
NlaIII CATG 5 cut(s) 87, 205, 279, 651, 781
NlaIV GGNNCC 1 cut(s) 238
NmuCI GTSAC 1 cut(s) 241
NsiI ATGCAT 1 cut(s) 653
NspI RCATGY 2 cut(s) 205, 651
PaeI GCATGC 1 cut(s) 651
PaeR7I CTCGAG 1 cut(s) 541
PasI CCCWGGG 1 cut(s) 817
PctI GAATGC 3 cut(s) 559, 607, 839
PfeI GAWTC 3 cut(s) 167, 433, 810
PflMI CCANNNNNTGG 1 cut(s) 429
PkrI GCNGC 3 cut(s) 207, 607, 839
Psp6I CCWGG 1 cut(s) 816
PspGI CCWGG 1 cut(s) 816
PspN4I GGNNCC 1 cut(s) 238
PspXI VCTCGAGB 1 cut(s) 541
PvuII CAGCTG 1 cut(s) 53
RsaI GTAC 1 cut(s) 466
RsaNI GTAC 1 cut(s) 465
RseI CAYNNNNRTG 1 cut(s) 339
SaqAI TTAA 1 cut(s) 257
SatI GCNGC 3 cut(s) 206, 606, 838
Sau3AI GATC 2 cut(s) 665, 780
ScrFI CCNGG 1 cut(s) 818
SduI GDGCHC 1 cut(s) 694
Sfr274I CTCGAG 1 cut(s) 541
SlaI CTCGAG 1 cut(s) 541
SmiMI CAYNNNNRTG 1 cut(s) 339
SmlI CTYRAG 2 cut(s) 209, 541
SmoI CTYRAG 2 cut(s) 209, 541
SphI GCATGC 1 cut(s) 651
Sse9I AATT 3 cut(s) 377, 631, 728
SsiI CCGC 2 cut(s) 246, 678
SspMI CTAG 1 cut(s) 741
StyD4I CCNGG 1 cut(s) 816
StyI CCWWGG 1 cut(s) 352
TaaI ACNGT 2 cut(s) 95, 241
TaiI ACGT 1 cut(s) 586
TaqI TCGA 5 cut(s) 97, 141, 393, 542, 701
TasI AATT 3 cut(s) 377, 631, 728
TfiI GAWTC 3 cut(s) 167, 433, 810
Tru1I TTAA 1 cut(s) 257
Tru9I TTAA 1 cut(s) 257
TscAI CASTG 3 cut(s) 622, 694, 742
TseFI GTSAC 1 cut(s) 241
TseI GCWGC 3 cut(s) 205, 605, 837
Tsp45I GTSAC 1 cut(s) 241
TspDTI ATGAA 2 cut(s) 762, 834
TspGWI ACGGA 1 cut(s) 600
TspRI CASTG 3 cut(s) 622, 694, 742
Van91I CCANNNNNTGG 1 cut(s) 429
XagI CCTNNNNNAGG 1 cut(s) 625
XapI RAATTY 1 cut(s) 631
XceI RCATGY 2 cut(s) 205, 651
XhoI CTCGAG 1 cut(s) 541
XspI CTAG 1 cut(s) 741
Zsp2I ATGCAT 1 cut(s) 653
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.