FvH4_3g30171

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0000668
Physical Location & Seq
Forward (+)
23396353 .. 23397663
1311 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g30171.t1

Sequence Viewer

Length: 1215 bp
ATGGGTGCATCCAATTGTAGTACTCCTCCTATTCATTCAAAAATATCAAAATCCACAGATGTTATTGTTAGCTTTGATGATCTATTGGTTGAAATCCTATGTCGACTTCCTAGCAAGGAATTCGTTTTCCAATGCAAATGTGTTTCCAAGCATTGGCTCAGTCTCATCTCGGATCCGTATTTAATCGGCCGCTTTCTGCATCTTCAAAGATTTCATAATCAAACCCCAACAGCACGAACTCTGATAGACAGAAGAGGAAAGGAGTTCCTGACACCGTCGTCATCCAATATGCTATCTCCACTATTCAAAAGACTCATGAGTTTCCATGGTTTGGAAGAAAAGCCATATGTGGTAGCAACATCTAATGATTTAGTTTTGTGCTGCCGAACTCCATGTCACCAAAACCAACGTGAATTCTACATTTGCAATCCATGCACAAGGCAATGGATTGCTCTTCCTCCCACTACTCGATGTTGCAAGTATGCACCAGTTGGATTCATTTTTGATCGCTACTATGAAGAGGAAGATAGTACTACTAGTAGTACCAATAGTTTTGTTAATATTAGAAAGGACTACATGTGCAAGGTCGTGAGAATTCTTCCTCAAGATTTAGCTGCAACTTCTTTTGATTTGATGGGTCAACACTTTTCCAAATTTATTGTTCATGTGTTCTCTTTCGAAACGGGTGAATGGAGAGAGTCATTTGAGTCATCCCTGCGAAGCATCACAATTACAGACTCTAATAGCGTTGCTTACAATGGAATGTTGTATTGGACATATTGGGCATATGATGATGATGAGTCTCGTGAGGAGACGGGGCTTGTTGCGTTGGATCCTTTCATGATCGGCAATGTCAGTAGTGGTAGGTCTAATGGTGATGAGACTCTTGAAGATGATCGGTACCAATTTCATCTATGTGATGCAGAGTTGATTGCAGGTGATGATGATGGTCTTGGATCATCAGCTGACAAATTCTGTTTGAAAAAAGCGAGTACTGTTTATCGAGTAAGCTCGAAATTTGATATATTTGGTAATGGAGTTCTTCTTCGAGCTTTGGATCCGAATGATGAAGATATCTTTTATGTAGAAAGCTATCCATATATACTATTACATGTACAACATTCGTACAAGAAGGTGGTCAGAAGTAGCTTACGACATAATGCTTCCTCAATTATGTGTAATGGTGCTCCCATGGTGGCCAACACCACTTCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like

Protein Analysis

405

Amino Acids

46.01

Weight (kDa)

5.86

Isoelectric Point (pI)

40.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 26 - 61 2.2e-06 F-box domain
b-prop_At3g26010-like PF24750 113 - 279 3.5e-10 F-box protein At3g26010-like, beta-propeller
FBA_3 PF08268 132 - 266 3.1e-06 F-box associated beta propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 926
AasI GACNNNNNNGTC 1 cut(s) 277
Acc36I ACCTGC 1 cut(s) 926
Acc65I GGTACC 1 cut(s) 900
AccB1I GGYRCC 1 cut(s) 900
AccB7I CCANNNNNTGG 2 cut(s) 153, 331
AccI GTMKAC 1 cut(s) 103
AciI CCGC 1 cut(s) 190
AclWI GGATC 7 cut(s) 167, 180, 827, 840, 964, 1052, 1065
AcoI YGGCCR 2 cut(s) 187, 1197
AcsI RAATTY 6 cut(s) 119, 413, 594, 653, 971, 1016
AfaI GTAC 7 cut(s) 22, 532, 544, 902, 994, 1116, 1127
AfiI CCNNNNNNNGG 2 cut(s) 153, 331
AflIII ACRYGT 2 cut(s) 576, 1111
AgsI TTSAA 6 cut(s) 39, 92, 206, 307, 890, 982
AhlI ACTAGT 1 cut(s) 536
AjuI GAANNNNNNNTTGG 2 cut(s) 754, 786
AluBI AGCT 7 cut(s) 72, 614, 965, 1011, 1052, 1092, 1149
AluI AGCT 7 cut(s) 72, 614, 965, 1011, 1052, 1092, 1149
Alw21I GWGCWC 1 cut(s) 1189
Alw26I GTCTC 4 cut(s) 167, 806, 807, 875
AlwI GGATC 7 cut(s) 167, 180, 827, 840, 964, 1052, 1065
AoxI GGCC 2 cut(s) 187, 1197
ApeKI GCWGC 2 cut(s) 381, 614
ApoI RAATTY 6 cut(s) 119, 413, 594, 653, 971, 1016
ArsI GACNNNNNNTTYG 2 cut(s) 379, 411
Asp718I GGTACC 1 cut(s) 900
AsuHPI GGTGA 4 cut(s) 389, 698, 887, 950
AsuII TTCGAA 1 cut(s) 678
BalI TGGCCA 1 cut(s) 1199
BamHI GGATCC 3 cut(s) 172, 832, 1057
BanI GGYRCC 1 cut(s) 900
BauI CACGAG 1 cut(s) 804
Bbv12I GWGCWC 1 cut(s) 1189
BbvI GCAGC 2 cut(s) 368, 601
BccI CCATC 2 cut(s) 628, 941
BcgI CGANNNNNNTGC 2 cut(s) 103, 137
BcoDI GTCTC 4 cut(s) 167, 806, 807, 875
BcuI ACTAGT 1 cut(s) 536
BfaI CTAG 2 cut(s) 111, 537
BfuAI ACCTGC 1 cut(s) 926
BisI GCNGC 3 cut(s) 190, 382, 615
BlsI GCNGC 3 cut(s) 191, 383, 616
BmcAI AGTACT 3 cut(s) 22, 532, 994
BmiI GGNNCC 4 cut(s) 174, 834, 902, 1059
BmsI GCATC 4 cut(s) 17, 208, 732, 910
Bpu14I TTCGAA 1 cut(s) 678
BpuEI CTTGAG 1 cut(s) 588
BsaJI CCNNGG 2 cut(s) 325, 1191
Bsc4I CCNNNNNNNGG 2 cut(s) 153, 331
Bse1I ACTGG 1 cut(s) 488
Bse3DI GCAATG 2 cut(s) 449, 856
BseDI CCNNGG 2 cut(s) 325, 1191
BseGI GGATG 3 cut(s) 8, 281, 710
BseLI CCNNNNNNNGG 2 cut(s) 153, 331
BseMI GCAATG 2 cut(s) 449, 856
BseMII CTCAG 1 cut(s) 172
BseNI ACTGG 1 cut(s) 488
BseRI GAGGAG 2 cut(s) 15, 824
BseX3I CGGCCG 1 cut(s) 187
BseXI GCAGC 2 cut(s) 368, 601
Bsh1285I CGRYCG 1 cut(s) 190
BshFI GGCC 2 cut(s) 189, 1199
BshNI GGYRCC 1 cut(s) 900
BsiEI CGRYCG 1 cut(s) 190
BsiHKAI GWGCWC 1 cut(s) 1189
BslI CCNNNNNNNGG 2 cut(s) 153, 331
BsmAI GTCTC 4 cut(s) 167, 806, 807, 875
BsmBI CGTCTC 1 cut(s) 806
BsnI GGCC 2 cut(s) 189, 1199
Bsp119I TTCGAA 1 cut(s) 678
Bsp1286I GDGCHC 1 cut(s) 1189
Bsp1407I TGTACA 1 cut(s) 1114
Bsp143I GATC 8 cut(s) 79, 172, 505, 832, 843, 895, 956, 1057
Bsp19I CCATGG 2 cut(s) 325, 1191
BspACI CCGC 1 cut(s) 190
BspANI GGCC 2 cut(s) 189, 1199
BspCNI CTCAG 1 cut(s) 171
BspHI TCATGA 2 cut(s) 315, 840
BspLI GGNNCC 4 cut(s) 174, 834, 902, 1059
BspMI ACCTGC 1 cut(s) 926
BspPI GGATC 7 cut(s) 167, 180, 827, 840, 964, 1052, 1065
BspQI GCTCTTC 1 cut(s) 459
BspT104I TTCGAA 1 cut(s) 678
BspT107I GGYRCC 1 cut(s) 900
BsrDI GCAATG 2 cut(s) 449, 856
BsrGI TGTACA 1 cut(s) 1114
BsrI ACTGG 1 cut(s) 488
BssECI CCNNGG 2 cut(s) 325, 1191
BssMI GATC 8 cut(s) 79, 172, 505, 832, 843, 895, 956, 1057
BssSI CACGAG 1 cut(s) 804
BssT1I CCWWGG 2 cut(s) 325, 1191
Bst2BI CACGAG 1 cut(s) 804
Bst4CI ACNGT 2 cut(s) 276, 997
Bst6I CTCTTC 3 cut(s) 247, 459, 513
BstAPI GCANNNNNTGC 1 cut(s) 432
BstAUI TGTACA 1 cut(s) 1114
BstBI TTCGAA 1 cut(s) 678
BstDEI CTNAG 1 cut(s) 158
BstDSI CCRYGG 2 cut(s) 325, 1191
BstF5I GGATG 3 cut(s) 8, 281, 710
BstKTI GATC 8 cut(s) 82, 175, 508, 835, 846, 898, 959, 1060
BstMAI GTCTC 4 cut(s) 167, 806, 807, 875
BstMBI GATC 8 cut(s) 79, 172, 505, 832, 843, 895, 956, 1057
BstMCI CGRYCG 1 cut(s) 190
BstMWI GCNNNNNNNGC 1 cut(s) 432
BstNSI RCATGY 2 cut(s) 580, 1115
BstV1I GCAGC 2 cut(s) 368, 601
BstX2I RGATCY 3 cut(s) 172, 832, 1057
BstYI RGATCY 3 cut(s) 172, 832, 1057
BstZI CGGCCG 1 cut(s) 187
BsuRI GGCC 2 cut(s) 189, 1199
BtgI CCRYGG 2 cut(s) 325, 1191
BtsCI GGATG 3 cut(s) 8, 281, 710
BveI ACCTGC 1 cut(s) 926
CciI TCATGA 2 cut(s) 315, 840
Csp6I GTAC 7 cut(s) 21, 531, 543, 901, 993, 1115, 1126
CviAII CATG 9 cut(s) 316, 326, 393, 432, 577, 665, 841, 1112, 1192
CviQI GTAC 7 cut(s) 21, 531, 543, 901, 993, 1115, 1126
DdeI CTNAG 1 cut(s) 158
DpnI GATC 8 cut(s) 81, 174, 507, 834, 845, 897, 958, 1059
DpnII GATC 8 cut(s) 79, 172, 505, 832, 843, 895, 956, 1057
DrdI GACNNNNNNGTC 1 cut(s) 277
DseDI GACNNNNNNGTC 1 cut(s) 277
EaeI YGGCCR 2 cut(s) 187, 1197
EagI CGGCCG 1 cut(s) 187
Eam1104I CTCTTC 3 cut(s) 247, 459, 513
EarI CTCTTC 3 cut(s) 247, 459, 513
EclXI CGGCCG 1 cut(s) 187
Eco130I CCWWGG 2 cut(s) 325, 1191
Eco32I GATATC 1 cut(s) 1075
Eco52I CGGCCG 1 cut(s) 187
EcoRI GAATTC 3 cut(s) 119, 413, 594
EcoRV GATATC 1 cut(s) 1075
EcoT14I CCWWGG 2 cut(s) 325, 1191
ErhI CCWWGG 2 cut(s) 325, 1191
Esp3I CGTCTC 1 cut(s) 806
FaeI CATG 9 cut(s) 319, 329, 396, 435, 580, 668, 844, 1115, 1195
FatI CATG 9 cut(s) 315, 325, 392, 431, 576, 664, 840, 1111, 1191
FauNDI CATATG 2 cut(s) 346, 787
FblI GTMKAC 1 cut(s) 103
Fnu4HI GCNGC 3 cut(s) 190, 382, 615
FokI GGATG 2 cut(s) 268, 697
Fsp4HI GCNGC 3 cut(s) 190, 382, 615
FspBI CTAG 2 cut(s) 111, 537
GluI GCNGC 3 cut(s) 190, 382, 615
HaeIII GGCC 2 cut(s) 189, 1199
Hin1II CATG 9 cut(s) 319, 329, 396, 435, 580, 668, 844, 1115, 1195
HincII GTYRAC 2 cut(s) 104, 641
HindII GTYRAC 2 cut(s) 104, 641
HinfI GANTC 7 cut(s) 312, 495, 698, 707, 737, 800, 883
HphI GGTGA 4 cut(s) 389, 698, 887, 950
Hpy166II GTNNAC 2 cut(s) 104, 641
Hpy188I TCNGA 4 cut(s) 172, 243, 1062, 1142
Hpy188III TCNNGA 7 cut(s) 268, 316, 589, 605, 806, 841, 887
Hpy8I GTNNAC 2 cut(s) 104, 641
Hpy99I CGWCG 1 cut(s) 280
HpyAV CCTTC 1 cut(s) 1126
HpyCH4III ACNGT 2 cut(s) 276, 997
HpyCH4IV ACGT 1 cut(s) 409
HpyF10VI GCNNNNNNNGC 1 cut(s) 432
HpyF3I CTNAG 1 cut(s) 158
HpySE526I ACGT 1 cut(s) 409
Hsp92II CATG 9 cut(s) 319, 329, 396, 435, 580, 668, 844, 1115, 1195
KpnI GGTACC 1 cut(s) 904
Kzo9I GATC 8 cut(s) 79, 172, 505, 832, 843, 895, 956, 1057
LguI GCTCTTC 1 cut(s) 459
LmnI GCTCC 1 cut(s) 1192
LpnPI CCDG 4 cut(s) 281, 501, 728, 921
Lsp1109I GCAGC 2 cut(s) 368, 601
LweI GCATC 4 cut(s) 17, 208, 732, 910
MaeI CTAG 2 cut(s) 111, 537
MaeII ACGT 1 cut(s) 409
MaeIII GTNAC 1 cut(s) 395
MalI GATC 8 cut(s) 81, 174, 507, 834, 845, 897, 958, 1059
MboI GATC 8 cut(s) 79, 172, 505, 832, 843, 895, 956, 1057
MfeI CAATTG 1 cut(s) 13
MflI RGATCY 3 cut(s) 172, 832, 1057
MhlI GDGCHC 1 cut(s) 1189
MlsI TGGCCA 1 cut(s) 1199
MluNI TGGCCA 1 cut(s) 1199
MlyI GAGTC 6 cut(s) 306, 707, 716, 731, 809, 877
MmeI TCCRAC 2 cut(s) 472, 810
MnlI CCTC 7 cut(s) 36, 248, 468, 514, 612, 802, 1177
Mox20I TGGCCA 1 cut(s) 1199
MscI TGGCCA 1 cut(s) 1199
MseI TTAA 2 cut(s) 182, 558
MslI CAYNNNNRTG 1 cut(s) 915
Msp20I TGGCCA 1 cut(s) 1199
MspA1I CMGCKG 1 cut(s) 965
MunI CAATTG 1 cut(s) 13
MwoI GCNNNNNNNGC 1 cut(s) 432
NcoI CCATGG 2 cut(s) 325, 1191
NdeI CATATG 2 cut(s) 346, 787
NdeII GATC 8 cut(s) 79, 172, 505, 832, 843, 895, 956, 1057
NlaIII CATG 9 cut(s) 319, 329, 396, 435, 580, 668, 844, 1115, 1195
NlaIV GGNNCC 4 cut(s) 174, 834, 902, 1059
NmuCI GTSAC 1 cut(s) 395
NspI RCATGY 2 cut(s) 580, 1115
NspV TTCGAA 1 cut(s) 678
PagI TCATGA 2 cut(s) 315, 840
PaqCI CACCTGC 1 cut(s) 926
PciI ACATGT 2 cut(s) 576, 1111
PciSI GCTCTTC 1 cut(s) 459
PfeI GAWTC 1 cut(s) 495
PflFI GACNNNGTC 1 cut(s) 274
PflMI CCANNNNNTGG 2 cut(s) 153, 331
PkrI GCNGC 3 cut(s) 191, 383, 616
PleI GAGTC 6 cut(s) 306, 706, 715, 731, 808, 877
PpsI GAGTC 6 cut(s) 306, 706, 715, 731, 808, 877
PscI ACATGT 2 cut(s) 576, 1111
PspN4I GGNNCC 4 cut(s) 174, 834, 902, 1059
PsuI RGATCY 3 cut(s) 172, 832, 1057
PsyI GACNNNGTC 1 cut(s) 274
PvuII CAGCTG 1 cut(s) 965
RsaI GTAC 7 cut(s) 22, 532, 544, 902, 994, 1116, 1127
RsaNI GTAC 7 cut(s) 21, 531, 543, 901, 993, 1115, 1126
RseI CAYNNNNRTG 1 cut(s) 915
SalI GTCGAC 1 cut(s) 102
SapI GCTCTTC 1 cut(s) 459
SaqAI TTAA 2 cut(s) 182, 558
SatI GCNGC 3 cut(s) 190, 382, 615
Sau3AI GATC 8 cut(s) 79, 172, 505, 832, 843, 895, 956, 1057
ScaI AGTACT 3 cut(s) 22, 532, 994
SchI GAGTC 6 cut(s) 306, 707, 716, 731, 809, 877
SduI GDGCHC 1 cut(s) 1189
SfaNI GCATC 4 cut(s) 17, 208, 732, 910
SfuI TTCGAA 1 cut(s) 678
SmiMI CAYNNNNRTG 1 cut(s) 915
SmlI CTYRAG 1 cut(s) 603
SmoI CTYRAG 1 cut(s) 603
SpeI ACTAGT 1 cut(s) 536
SsiI CCGC 1 cut(s) 190
SspI AATATT 1 cut(s) 562
SspMI CTAG 2 cut(s) 111, 537
StyI CCWWGG 2 cut(s) 325, 1191
TaaI ACNGT 2 cut(s) 276, 997
TaiI ACGT 1 cut(s) 412
TaqI TCGA 6 cut(s) 103, 469, 678, 1003, 1013, 1048
TatI WGTACW 4 cut(s) 20, 530, 992, 1114
TauI GCSGC 1 cut(s) 192
TfiI GAWTC 1 cut(s) 495
Tru1I TTAA 2 cut(s) 182, 558
Tru9I TTAA 2 cut(s) 182, 558
TseFI GTSAC 1 cut(s) 395
TseI GCWGC 2 cut(s) 381, 614
Tsp45I GTSAC 1 cut(s) 395
TspDTI ATGAA 8 cut(s) 23, 203, 487, 531, 653, 829, 899, 1083
TspGWI ACGGA 1 cut(s) 165
Tth111I GACNNNGTC 1 cut(s) 274
Van91I CCANNNNNTGG 2 cut(s) 153, 331
XapI RAATTY 6 cut(s) 119, 413, 594, 653, 971, 1016
XceI RCATGY 2 cut(s) 580, 1115
XmiI GTMKAC 1 cut(s) 103
XspI CTAG 2 cut(s) 111, 537
ZrmI AGTACT 3 cut(s) 22, 532, 994
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.