FvH4_3g44471

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0000668
Physical Location & Seq
Forward (+)
37085896 .. 37087786
1891 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g44471.t1

Sequence Viewer

Length: 1512 bp
ATGCATGGCTTGCATTGCATTGATCCGTTCTTTAATTTGTTCGTGATTCAGAATCAGAAGATGAAGACCAAAAGATCCAAGGAGCTAGATACTCCGGCAATTAAAGACGAGGCTGAAACTGGTATTTTGAGTGAATGGGAGAAGAGTTCATCAGACAAACCATCATACGATAGTACTCCTATTCAATCAAAAAGATCAAAATCCACAGATGTCGTTAGCTTTGATGATCTCCCTGACCTTCTGTCGGTTGAAATCCTATGTCGGCTTCCTAGCAAGGAATTAGTTTTTCAATGCAAGTGTGTGTCCAAGCATTGGCTCAATCTCATCTCGGATCCGTATTTCATTGGCCGCTTTCTTCATCTTCAAAGATTTCATAAGCAAACCCGAATAGCACGAACTCTGATAGACAGAAGAGGAGAGGAATTCCCTCCAACGACAGAGTGGTCGTCACCATCCAATCTGCTAACTCCGCTGTTCAAAAGACTCATGAGTTTCCACCGTTTGAAAGAAAATCCGTATGTGGTAGCAACATCTAATGATTTAGTTTTGTGCTGCCGAACTCCGTTTAACAAAAACCAGCGTGATTTCTACATTTGCAATCCATGCACAAGGCAATGGATTGCTCTTCCTCCCACCACTCGACGTTGCAAGTATGCACCAGTCGGATTCATATGTGATCTGTACTATGAGGAGGAAGATGGTAGTGTTAGTATTAAAAAGGAGTACAGGTGCATGGTCGTGAGAATTCTTCCTCACGATTTAGCTACAACATCTCTGAAGTGGGGTGAACATTGCAAATTCATTGTTGAGGTATTCTCTTTCGAGACGGGTGAATGGAGAGAGTCAATTGTATCATCCCCACGAAGCATCAGGTTTGCAGATGCTACTAGGAGTACTAGCGTAGCTCACAAAGGAATGCTGTATTGGACATATGTAGCGTATGAGCCGCGTTACGAGATCGGGGTTATTGCTTTGGATCCTTTCGTGATCGGCATTAATGGCAGTAGAAATGGTGGTGAGACTACTGTTGATCACTATGATCATTACCAATTTCGTCTCTCTGTTGGTATGGATTCATCTGGAGACGCCAGTGGTCTTATGTGTGTGCGCCAAGGACGTCTGCATATTTTGACCTACAGTTGCTGGAAGGCCGAGTTTATGGTTTGGGAATTGACAGATGATCTTGGATCAGCTGACGGATTCTATTTAAGAAGAGTGAGTGTCCATCCACGACGCCTTGAAGTTTGTCTATATCATGATCATCATCGTGTTGTTCTTCTAACTTTGGACCCGAATGATGTGGATATATTCTATGTAAAAGAGCGTACTGGTAACTATGTCAGGAGATACGATACTGACGTCTCCTACGAATACCATTTCAAGATCGACGTGGGTACAAGAAAGTGCTCAAGAGTAGGTGAGGCCAAGTATTTTGAAGACAGTCATTTCTATCCATTGGTGCTCCCATGGTGGCCAACACCACTTCGTAGGCTACCCATATGTGCTAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like

Protein Analysis

504

Amino Acids

58.6

Weight (kDa)

8.04

Isoelectric Point (pI)

47.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 74 - 114 6.8e-07 F-box domain
b-prop_At3g26010-like PF24750 163 - 328 5.6e-13 F-box protein At3g26010-like, beta-propeller
FBA_1 PF07734 171 - 399 1.5e-07 F-box associated beta propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 442
AatII GACGTC 2 cut(s) 1120, 1362
AccB7I CCANNNNNTGG 1 cut(s) 312
AccII CGCG 1 cut(s) 949
AciI CCGC 3 cut(s) 349, 470, 947
AclWI GGATC 7 cut(s) 17, 69, 326, 339, 971, 984, 1195
AcoI YGGCCR 2 cut(s) 346, 1472
AcsI RAATTY 3 cut(s) 422, 744, 797
AcuI CTGAAG 1 cut(s) 797
AcyI GRCGYC 4 cut(s) 1086, 1117, 1234, 1359
AfaI GTAC 6 cut(s) 175, 683, 725, 895, 1327, 1396
AfiI CCNNNNNNNGG 2 cut(s) 244, 312
AgsI TTSAA 9 cut(s) 185, 251, 290, 365, 478, 505, 1241, 1381, 1436
AhdI GACNNNNNGTC 1 cut(s) 241
AjiI CACGTC 1 cut(s) 1390
AjuI GAANNNNNNNTTGG 2 cut(s) 907, 939
AluBI AGCT 6 cut(s) 85, 219, 764, 905, 1193, 1509
AluI AGCT 6 cut(s) 85, 219, 764, 905, 1193, 1509
Alw21I GWGCWC 2 cut(s) 1409, 1464
Alw26I GTCTC 5 cut(s) 818, 1013, 1061, 1077, 1366
AlwI GGATC 7 cut(s) 17, 69, 326, 339, 971, 984, 1195
AlwNI CAGNNNCTG 1 cut(s) 1143
AoxI GGCC 4 cut(s) 346, 1149, 1422, 1472
ApeKI GCWGC 1 cut(s) 552
ApoI RAATTY 3 cut(s) 422, 744, 797
AseI ATTAAT 1 cut(s) 996
AspLEI GCGC 1 cut(s) 1110
AspS9I GGNCC 1 cut(s) 1288
AsuHPI GGTGA 5 cut(s) 441, 797, 842, 1028, 1430
AsuNHI GCTAGC 1 cut(s) 1505
AvaII GGWCC 1 cut(s) 1288
BaeI ACNNNNGTAYC 2 cut(s) 1344, 1377
BalI TGGCCA 1 cut(s) 1474
BamHI GGATCC 2 cut(s) 331, 976
BbsI GAAGAC 2 cut(s) 71, 1443
Bbv12I GWGCWC 2 cut(s) 1409, 1464
BbvI GCAGC 1 cut(s) 539
BccI CCATC 4 cut(s) 169, 460, 692, 1233
BclI TGATCA 3 cut(s) 1030, 1039, 1258
BcoDI GTCTC 5 cut(s) 818, 1013, 1061, 1077, 1366
BfaI CTAG 6 cut(s) 86, 270, 888, 897, 1506, 1510
BfmI CTRYAG 1 cut(s) 1135
BisI GCNGC 3 cut(s) 349, 553, 947
BlsI GCNGC 3 cut(s) 350, 554, 948
BmcAI AGTACT 2 cut(s) 175, 895
Bme18I GGWCC 1 cut(s) 1288
BmeRI GACNNNNNGTC 1 cut(s) 241
BmgBI CACGTC 1 cut(s) 1390
BmgT120I GGNCC 1 cut(s) 1288
BmiI GGNNCC 3 cut(s) 333, 978, 1290
BmsI GCATC 2 cut(s) 871, 876
BmtI GCTAGC 1 cut(s) 1509
BpiI GAAGAC 2 cut(s) 71, 1443
BplI GAGNNNNNCTC 2 cut(s) 800, 832
BpmI CTGGAG 1 cut(s) 1101
BpuEI CTTGAG 1 cut(s) 1393
BsaBI GATNNNNATC 2 cut(s) 199, 1263
BsaHI GRCGYC 4 cut(s) 1086, 1117, 1234, 1359
BsaJI CCNNGG 3 cut(s) 78, 1111, 1466
BsaXI ACNNNNNCTCC 2 cut(s) 713, 743
Bsc4I CCNNNNNNNGG 2 cut(s) 244, 312
Bse1I ACTGG 4 cut(s) 124, 659, 1089, 1333
Bse3DI GCAATG 3 cut(s) 13, 620, 790
Bse8I GATNNNNATC 2 cut(s) 199, 1263
BseDI CCNNGG 3 cut(s) 78, 1111, 1466
BseGI GGATG 3 cut(s) 452, 854, 1225
BseJI GATNNNNATC 2 cut(s) 199, 1263
BseLI CCNNNNNNNGG 2 cut(s) 244, 312
BseMI GCAATG 3 cut(s) 13, 620, 790
BseNI ACTGG 4 cut(s) 124, 659, 1089, 1333
BseRI GAGGAG 2 cut(s) 429, 704
BseXI GCAGC 1 cut(s) 539
Bsh1236I CGCG 1 cut(s) 949
BshFI GGCC 4 cut(s) 348, 1151, 1424, 1474
BsiHKAI GWGCWC 2 cut(s) 1409, 1464
BsiSI CCGG 1 cut(s) 95
BslI CCNNNNNNNGG 2 cut(s) 244, 312
BsmAI GTCTC 5 cut(s) 818, 1013, 1061, 1077, 1366
BsmBI CGTCTC 4 cut(s) 818, 1061, 1077, 1366
BsmI GAATGC 1 cut(s) 921
BsnI GGCC 4 cut(s) 348, 1151, 1424, 1474
Bsp1286I GDGCHC 2 cut(s) 1409, 1464
Bsp19I CCATGG 1 cut(s) 1466
BspACI CCGC 3 cut(s) 349, 470, 947
BspANI GGCC 4 cut(s) 348, 1151, 1424, 1474
BspFNI CGCG 1 cut(s) 949
BspHI TCATGA 2 cut(s) 486, 1255
BspLI GGNNCC 3 cut(s) 333, 978, 1290
BspOI GCTAGC 1 cut(s) 1509
BspPI GGATC 7 cut(s) 17, 69, 326, 339, 971, 984, 1195
BspQI GCTCTTC 1 cut(s) 630
BsrDI GCAATG 3 cut(s) 13, 620, 790
BsrI ACTGG 4 cut(s) 124, 659, 1089, 1333
BssECI CCNNGG 3 cut(s) 78, 1111, 1466
BssNI GRCGYC 4 cut(s) 1086, 1117, 1234, 1359
BssT1I CCWWGG 3 cut(s) 78, 1111, 1466
Bst4CI ACNGT 4 cut(s) 500, 1027, 1139, 1442
Bst6I CTCTTC 4 cut(s) 137, 406, 630, 1207
BstACI GRCGYC 4 cut(s) 1086, 1117, 1234, 1359
BstAPI GCANNNNNTGC 2 cut(s) 10, 603
BstC8I GCNNGC 2 cut(s) 11, 1507
BstDSI CCRYGG 1 cut(s) 1466
BstF5I GGATG 3 cut(s) 452, 854, 1225
BstFNI CGCG 1 cut(s) 949
BstHHI GCGC 1 cut(s) 1110
BstMAI GTCTC 5 cut(s) 818, 1013, 1061, 1077, 1366
BstMWI GCNNNNNNNGC 5 cut(s) 10, 15, 469, 603, 999
BstSFI CTRYAG 1 cut(s) 1135
BstUI CGCG 1 cut(s) 949
BstV1I GCAGC 1 cut(s) 539
BstV2I GAAGAC 2 cut(s) 71, 1443
BstX2I RGATCY 3 cut(s) 74, 331, 976
BstYI RGATCY 3 cut(s) 74, 331, 976
BsuRI GGCC 4 cut(s) 348, 1151, 1424, 1474
BtgI CCRYGG 1 cut(s) 1466
BtrI CACGTC 1 cut(s) 1390
BtsCI GGATG 3 cut(s) 452, 854, 1225
BtsIMutI CAGTG 1 cut(s) 1096
Cac8I GCNNGC 2 cut(s) 11, 1507
CaiI CAGNNNCTG 1 cut(s) 1143
CciI TCATGA 2 cut(s) 486, 1255
CfoI GCGC 1 cut(s) 1110
Cfr13I GGNCC 1 cut(s) 1288
CseI GACGC 2 cut(s) 1094, 1242
Csp6I GTAC 6 cut(s) 174, 682, 724, 894, 1326, 1395
CviAII CATG 6 cut(s) 5, 487, 603, 733, 1256, 1467
CviQI GTAC 6 cut(s) 174, 682, 724, 894, 1326, 1395
DrdI GACNNNNNNGTC 1 cut(s) 442
DriI GACNNNNNGTC 1 cut(s) 241
DseDI GACNNNNNNGTC 1 cut(s) 442
EaeI YGGCCR 2 cut(s) 346, 1472
Eam1104I CTCTTC 4 cut(s) 137, 406, 630, 1207
Eam1105I GACNNNNNGTC 1 cut(s) 241
EarI CTCTTC 4 cut(s) 137, 406, 630, 1207
Eco130I CCWWGG 3 cut(s) 78, 1111, 1466
Eco47I GGWCC 1 cut(s) 1288
Eco57I CTGAAG 1 cut(s) 797
EcoRI GAATTC 2 cut(s) 422, 744
EcoT14I CCWWGG 3 cut(s) 78, 1111, 1466
EcoT22I ATGCAT 1 cut(s) 6
ErhI CCWWGG 3 cut(s) 78, 1111, 1466
Esp3I CGTCTC 4 cut(s) 818, 1061, 1077, 1366
FaeI CATG 6 cut(s) 8, 490, 606, 736, 1259, 1470
FatI CATG 6 cut(s) 4, 486, 602, 732, 1255, 1466
FauNDI CATATG 3 cut(s) 671, 931, 1499
FbaI TGATCA 3 cut(s) 1030, 1039, 1258
Fnu4HI GCNGC 3 cut(s) 349, 553, 947
FokI GGATG 3 cut(s) 439, 841, 1212
Fsp4HI GCNGC 3 cut(s) 349, 553, 947
FspBI CTAG 6 cut(s) 86, 270, 888, 897, 1506, 1510
GlaI GCGC 1 cut(s) 1109
GluI GCNGC 3 cut(s) 349, 553, 947
GsuI CTGGAG 1 cut(s) 1101
HaeIII GGCC 4 cut(s) 348, 1151, 1424, 1474
HapII CCGG 1 cut(s) 95
HgaI GACGC 2 cut(s) 1094, 1242
HhaI GCGC 1 cut(s) 1110
Hin1I GRCGYC 4 cut(s) 1086, 1117, 1234, 1359
Hin1II CATG 6 cut(s) 8, 490, 606, 736, 1259, 1470
Hin6I GCGC 1 cut(s) 1108
HinP1I GCGC 1 cut(s) 1108
HinfI GANTC 7 cut(s) 46, 52, 483, 666, 842, 1073, 1200
HpaII CCGG 1 cut(s) 95
HphI GGTGA 5 cut(s) 441, 797, 842, 1028, 1430
Hpy166II GTNNAC 1 cut(s) 788
Hpy188I TCNGA 7 cut(s) 51, 57, 154, 331, 402, 665, 777
Hpy8I GTNNAC 1 cut(s) 788
Hpy99I CGWCG 3 cut(s) 645, 1236, 1391
HpyAV CCTTC 2 cut(s) 248, 1141
HpyCH4III ACNGT 4 cut(s) 500, 1027, 1139, 1442
HpyCH4IV ACGT 4 cut(s) 643, 1117, 1359, 1389
HpyF10VI GCNNNNNNNGC 5 cut(s) 10, 15, 469, 603, 999
HpySE526I ACGT 4 cut(s) 643, 1117, 1359, 1389
Hsp92I GRCGYC 4 cut(s) 1086, 1117, 1234, 1359
Hsp92II CATG 6 cut(s) 8, 490, 606, 736, 1259, 1470
HspAI GCGC 1 cut(s) 1108
Ksp22I TGATCA 3 cut(s) 1030, 1039, 1258
LguI GCTCTTC 1 cut(s) 630
LmnI GCTCC 2 cut(s) 82, 1467
Lsp1109I GCAGC 1 cut(s) 539
LweI GCATC 2 cut(s) 871, 876
MaeI CTAG 6 cut(s) 86, 270, 888, 897, 1506, 1510
MaeII ACGT 4 cut(s) 643, 1117, 1359, 1389
MaeIII GTNAC 3 cut(s) 447, 950, 1331
MfeI CAATTG 1 cut(s) 846
MflI RGATCY 3 cut(s) 74, 331, 976
MhlI GDGCHC 2 cut(s) 1409, 1464
MlsI TGGCCA 1 cut(s) 1474
MluCI AATT 9 cut(s) 34, 99, 278, 422, 744, 797, 846, 1049, 1169
MluNI TGGCCA 1 cut(s) 1474
MlyI GAGTC 2 cut(s) 477, 851
MmeI TCCRAC 2 cut(s) 455, 643
Mox20I TGGCCA 1 cut(s) 1474
Mph1103I ATGCAT 1 cut(s) 6
MscI TGGCCA 1 cut(s) 1474
MseI TTAA 6 cut(s) 33, 102, 567, 714, 996, 1208
MslI CAYNNNNRTG 2 cut(s) 737, 1266
Msp20I TGGCCA 1 cut(s) 1474
MspA1I CMGCKG 2 cut(s) 472, 1193
MspI CCGG 1 cut(s) 95
MunI CAATTG 1 cut(s) 846
Mva1269I GAATGC 1 cut(s) 921
MvnI CGCG 1 cut(s) 949
MwoI GCNNNNNNNGC 5 cut(s) 10, 15, 469, 603, 999
NcoI CCATGG 1 cut(s) 1466
NdeI CATATG 3 cut(s) 671, 931, 1499
NheI GCTAGC 1 cut(s) 1505
NlaIII CATG 6 cut(s) 8, 490, 606, 736, 1259, 1470
NlaIV GGNNCC 3 cut(s) 333, 978, 1290
NmeAIII GCCGAG 1 cut(s) 1177
NmuCI GTSAC 1 cut(s) 447
NsiI ATGCAT 1 cut(s) 6
PagI TCATGA 2 cut(s) 486, 1255
PciSI GCTCTTC 1 cut(s) 630
PcsI WCGNNNNNNNCGW 2 cut(s) 1356, 1365
PctI GAATGC 1 cut(s) 921
PfeI GAWTC 5 cut(s) 46, 52, 666, 1073, 1200
PflMI CCANNNNNTGG 1 cut(s) 312
PkrI GCNGC 3 cut(s) 350, 554, 948
PleI GAGTC 2 cut(s) 477, 850
PpsI GAGTC 2 cut(s) 477, 850
PshBI ATTAAT 1 cut(s) 996
PspN4I GGNNCC 3 cut(s) 333, 978, 1290
PspPI GGNCC 1 cut(s) 1288
PstNI CAGNNNCTG 1 cut(s) 1143
PsuI RGATCY 3 cut(s) 74, 331, 976
PvuII CAGCTG 1 cut(s) 1193
RsaI GTAC 6 cut(s) 175, 683, 725, 895, 1327, 1396
RsaNI GTAC 6 cut(s) 174, 682, 724, 894, 1326, 1395
RseI CAYNNNNRTG 2 cut(s) 737, 1266
SapI GCTCTTC 1 cut(s) 630
SaqAI TTAA 6 cut(s) 33, 102, 567, 714, 996, 1208
SatI GCNGC 3 cut(s) 349, 553, 947
Sau96I GGNCC 1 cut(s) 1288
ScaI AGTACT 2 cut(s) 175, 895
SchI GAGTC 2 cut(s) 477, 851
SduI GDGCHC 2 cut(s) 1409, 1464
SfaNI GCATC 2 cut(s) 871, 876
SfcI CTRYAG 1 cut(s) 1135
SinI GGWCC 1 cut(s) 1288
SmiMI CAYNNNNRTG 2 cut(s) 737, 1266
SmlI CTYRAG 1 cut(s) 1408
SmoI CTYRAG 1 cut(s) 1408
Sse9I AATT 9 cut(s) 34, 99, 278, 422, 744, 797, 846, 1049, 1169
SsiI CCGC 3 cut(s) 349, 470, 947
SspMI CTAG 6 cut(s) 86, 270, 888, 897, 1506, 1510
StyI CCWWGG 3 cut(s) 78, 1111, 1466
TaaI ACNGT 4 cut(s) 500, 1027, 1139, 1442
TaiI ACGT 4 cut(s) 646, 1120, 1362, 1392
TaqI TCGA 3 cut(s) 640, 822, 1386
TasI AATT 9 cut(s) 34, 99, 278, 422, 744, 797, 846, 1049, 1169
TatI WGTACW 4 cut(s) 173, 681, 723, 893
TauI GCSGC 2 cut(s) 351, 949
TfiI GAWTC 5 cut(s) 46, 52, 666, 1073, 1200
Tru1I TTAA 6 cut(s) 33, 102, 567, 714, 996, 1208
Tru9I TTAA 6 cut(s) 33, 102, 567, 714, 996, 1208
TscAI CASTG 1 cut(s) 1096
TseFI GTSAC 1 cut(s) 447
TseI GCWGC 1 cut(s) 552
Tsp45I GTSAC 1 cut(s) 447
TspDTI ATGAA 8 cut(s) 77, 138, 331, 347, 362, 658, 790, 1065
TspGWI ACGGA 5 cut(s) 15, 324, 504, 552, 1212
TspRI CASTG 1 cut(s) 1096
Van91I CCANNNNNTGG 1 cut(s) 312
VpaK11BI GGWCC 1 cut(s) 1288
VspI ATTAAT 1 cut(s) 996
XapI RAATTY 3 cut(s) 422, 744, 797
XcmI CCANNNNNNNNNTGG 1 cut(s) 438
XspI CTAG 6 cut(s) 86, 270, 888, 897, 1506, 1510
ZraI GACGTC 2 cut(s) 1118, 1360
ZrmI AGTACT 2 cut(s) 175, 895
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.