Rh1DG124400

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0000668
Physical Location & Seq
Forward (+)
22612901 .. 22617063
4163 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG124400.1

Sequence Viewer

Length: 540 bp
ATGAAGACTAAAAAGTCAAGTAAGAAGGGCGCAGCACGGGGGCTAGATAGTTCGGCTATTAAAGACAAGGTTGAAATTGGTGTTTTGAGTGGAAGGGAAAAGAGATCATCAGAAGTATCACCCTCTGATGGTACTCATATTCACTCAACAAGATCAAAATCCACAAATGTTGTTAACATTGATGATCTCCCTGACGTTCTTCGTTGTTCCAAATTCATTGTTGAGATATTCTCTTTTGAGACTGGTGAATGGAGAGAGTCAATTGTATCATCCCCACGAAGCATTGGGTTTGGACCTGCTACTAGCGTTGCTTACAATGGAATGTTGTATTGGACATATAGGGACCAGAGCCAGACCTGGCTTATTAGGTTGGATCCTTTTGTGATCGGCAATGGCAGTGCTAGTGGTGGTAACCCTAATGGTGATGAGACTGTTGACGATGATCATTACCAATTTCGTCTCTTTGATGCAGTTGATATTGATTTAGACATGCCATGGATATGTATGCGTCGAGGGCGTCTGCAAATGGCTATATATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like

Protein Analysis

179

Amino Acids

19.99

Weight (kDa)

6.3

Isoelectric Point (pI)

45.07

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 13
Acc36I ACCTGC 1 cut(s) 304
AclWI GGATC 2 cut(s) 368, 381
AcsI RAATTY 1 cut(s) 212
AcyI GRCGYC 1 cut(s) 517
AfaI GTAC 1 cut(s) 133
AfiI CCNNNNNNNGG 1 cut(s) 128
AgsI TTSAA 1 cut(s) 74
AjnI CCWGG 1 cut(s) 356
AjuI GAANNNNNNNTTGG 2 cut(s) 313, 345
Alw26I GTCTC 3 cut(s) 233, 422, 464
AlwI GGATC 2 cut(s) 368, 381
ApeKI GCWGC 1 cut(s) 32
ApoI RAATTY 1 cut(s) 212
AspLEI GCGC 1 cut(s) 32
AspS9I GGNCC 2 cut(s) 293, 343
AsuHPI GGTGA 3 cut(s) 111, 257, 434
AvaII GGWCC 2 cut(s) 293, 343
BamHI GGATCC 1 cut(s) 373
BbsI GAAGAC 1 cut(s) 11
BbvI GCAGC 1 cut(s) 44
BccI CCATC 1 cut(s) 122
BciT130I CCWGG 1 cut(s) 358
BclI TGATCA 1 cut(s) 442
BcoDI GTCTC 3 cut(s) 233, 422, 464
BfaI CTAG 3 cut(s) 44, 303, 402
BfuAI ACCTGC 1 cut(s) 304
BisI GCNGC 1 cut(s) 33
BlsI GCNGC 1 cut(s) 34
Bme1390I CCNGG 1 cut(s) 358
Bme18I GGWCC 2 cut(s) 293, 343
BmgT120I GGNCC 2 cut(s) 293, 343
BmiI GGNNCC 2 cut(s) 344, 375
BmrFI CCNGG 1 cut(s) 358
BmsI GCATC 1 cut(s) 457
BpiI GAAGAC 1 cut(s) 11
BplI GAGNNNNNCTC 2 cut(s) 215, 247
BsaBI GATNNNNATC 1 cut(s) 157
BsaHI GRCGYC 1 cut(s) 517
BsaJI CCNNGG 1 cut(s) 494
Bsc4I CCNNNNNNNGG 1 cut(s) 128
Bse1I ACTGG 1 cut(s) 247
Bse3DI GCAATG 1 cut(s) 397
Bse8I GATNNNNATC 1 cut(s) 157
BseBI CCWGG 1 cut(s) 358
BseDI CCNNGG 1 cut(s) 494
BseGI GGATG 1 cut(s) 269
BseJI GATNNNNATC 1 cut(s) 157
BseLI CCNNNNNNNGG 1 cut(s) 128
BseMI GCAATG 1 cut(s) 397
BseNI ACTGG 1 cut(s) 247
BseXI GCAGC 1 cut(s) 44
BslFI GGGAC 1 cut(s) 356
BslI CCNNNNNNNGG 1 cut(s) 128
BsmAI GTCTC 3 cut(s) 233, 422, 464
BsmBI CGTCTC 1 cut(s) 464
BsmFI GGGAC 1 cut(s) 356
Bsp143I GATC 6 cut(s) 104, 152, 184, 373, 384, 442
Bsp19I CCATGG 1 cut(s) 494
BspLI GGNNCC 2 cut(s) 344, 375
BspMI ACCTGC 1 cut(s) 304
BspPI GGATC 2 cut(s) 368, 381
BsrDI GCAATG 1 cut(s) 397
BsrI ACTGG 1 cut(s) 247
BssECI CCNNGG 1 cut(s) 494
BssMI GATC 6 cut(s) 104, 152, 184, 373, 384, 442
BssNI GRCGYC 1 cut(s) 517
BssT1I CCWWGG 1 cut(s) 494
Bst2UI CCWGG 1 cut(s) 358
Bst4CI ACNGT 1 cut(s) 433
BstACI GRCGYC 1 cut(s) 517
BstDSI CCRYGG 1 cut(s) 494
BstEII GGTNACC 1 cut(s) 410
BstF5I GGATG 1 cut(s) 269
BstHHI GCGC 1 cut(s) 32
BstKTI GATC 6 cut(s) 107, 155, 187, 376, 387, 445
BstMAI GTCTC 3 cut(s) 233, 422, 464
BstMBI GATC 6 cut(s) 104, 152, 184, 373, 384, 442
BstMWI GCNNNNNNNGC 1 cut(s) 514
BstNI CCWGG 1 cut(s) 358
BstNSI RCATGY 1 cut(s) 493
BstPI GGTNACC 1 cut(s) 410
BstSCI CCNGG 1 cut(s) 356
BstV1I GCAGC 1 cut(s) 44
BstV2I GAAGAC 1 cut(s) 11
BstX2I RGATCY 1 cut(s) 373
BstYI RGATCY 1 cut(s) 373
BtgI CCRYGG 1 cut(s) 494
BtsCI GGATG 1 cut(s) 269
BtsI GCAGTG 1 cut(s) 403
BtsIMutI CAGTG 1 cut(s) 403
BveI ACCTGC 1 cut(s) 304
CfoI GCGC 1 cut(s) 32
Cfr13I GGNCC 2 cut(s) 293, 343
CseI GACGC 2 cut(s) 497, 506
Csp6I GTAC 1 cut(s) 132
CspCI CAANNNNNGTGG 2 cut(s) 151, 186
CviAII CATG 2 cut(s) 490, 495
CviJI RGCY 5 cut(s) 43, 56, 351, 361, 530
CviKI_1 RGCY 5 cut(s) 43, 56, 351, 361, 530
CviQI GTAC 1 cut(s) 132
DpnI GATC 6 cut(s) 106, 154, 186, 375, 386, 444
DpnII GATC 6 cut(s) 104, 152, 184, 373, 384, 442
DrdI GACNNNNNNGTC 1 cut(s) 13
DseDI GACNNNNNNGTC 1 cut(s) 13
Eco130I CCWWGG 1 cut(s) 494
Eco47I GGWCC 2 cut(s) 293, 343
Eco91I GGTNACC 1 cut(s) 410
EcoO65I GGTNACC 1 cut(s) 410
EcoRII CCWGG 1 cut(s) 356
EcoT14I CCWWGG 1 cut(s) 494
ErhI CCWWGG 1 cut(s) 494
Esp3I CGTCTC 1 cut(s) 464
FaeI CATG 2 cut(s) 493, 498
FaiI YATR 9 cut(s) 138, 337, 339, 491, 496, 502, 506, 533, 535
FaqI GGGAC 1 cut(s) 356
FatI CATG 2 cut(s) 489, 494
FbaI TGATCA 1 cut(s) 442
Fnu4HI GCNGC 1 cut(s) 33
FokI GGATG 1 cut(s) 256
Fsp4HI GCNGC 1 cut(s) 33
FspBI CTAG 3 cut(s) 44, 303, 402
GlaI GCGC 1 cut(s) 31
GluI GCNGC 1 cut(s) 33
HgaI GACGC 2 cut(s) 497, 506
HhaI GCGC 1 cut(s) 32
Hin1I GRCGYC 1 cut(s) 517
Hin1II CATG 2 cut(s) 493, 498
Hin6I GCGC 1 cut(s) 30
HinP1I GCGC 1 cut(s) 30
HincII GTYRAC 2 cut(s) 175, 436
HindII GTYRAC 2 cut(s) 175, 436
HinfI GANTC 1 cut(s) 257
HpaI GTTAAC 1 cut(s) 175
HphI GGTGA 3 cut(s) 111, 257, 434
Hpy166II GTNNAC 2 cut(s) 175, 436
Hpy188I TCNGA 2 cut(s) 112, 127
Hpy8I GTNNAC 2 cut(s) 175, 436
Hpy99I CGWCG 1 cut(s) 513
HpyAV CCTTC 2 cut(s) 19, 87
HpyCH4III ACNGT 1 cut(s) 433
HpyCH4IV ACGT 1 cut(s) 195
HpyCH4V TGCA 2 cut(s) 470, 523
HpyF10VI GCNNNNNNNGC 1 cut(s) 514
HpySE526I ACGT 1 cut(s) 195
Hsp92I GRCGYC 1 cut(s) 517
Hsp92II CATG 2 cut(s) 493, 498
HspAI GCGC 1 cut(s) 30
Ksp22I TGATCA 1 cut(s) 442
KspAI GTTAAC 1 cut(s) 175
Kzo9I GATC 6 cut(s) 104, 152, 184, 373, 384, 442
LpnPI CCDG 7 cut(s) 204, 228, 309, 343, 359, 365, 370
Lsp1109I GCAGC 1 cut(s) 44
LweI GCATC 1 cut(s) 457
MaeI CTAG 3 cut(s) 44, 303, 402
MaeII ACGT 1 cut(s) 195
MaeIII GTNAC 1 cut(s) 410
MalI GATC 6 cut(s) 106, 154, 186, 375, 386, 444
MboI GATC 6 cut(s) 104, 152, 184, 373, 384, 442
MboII GAAGA 2 cut(s) 16, 191
MfeI CAATTG 1 cut(s) 261
MflI RGATCY 1 cut(s) 373
MluCI AATT 4 cut(s) 75, 212, 261, 452
MlyI GAGTC 1 cut(s) 266
MmeI TCCRAC 1 cut(s) 351
MnlI CCTC 2 cut(s) 133, 506
MseI TTAA 2 cut(s) 60, 174
MslI CAYNNNNRTG 1 cut(s) 499
MspR9I CCNGG 1 cut(s) 358
MunI CAATTG 1 cut(s) 261
MvaI CCWGG 1 cut(s) 358
MwoI GCNNNNNNNGC 1 cut(s) 514
NcoI CCATGG 1 cut(s) 494
NdeII GATC 6 cut(s) 104, 152, 184, 373, 384, 442
NlaIII CATG 2 cut(s) 493, 498
NlaIV GGNNCC 2 cut(s) 344, 375
NspI RCATGY 1 cut(s) 493
PkrI GCNGC 1 cut(s) 34
PleI GAGTC 1 cut(s) 265
PpsI GAGTC 1 cut(s) 265
Psp6I CCWGG 1 cut(s) 356
PspEI GGTNACC 1 cut(s) 410
PspGI CCWGG 1 cut(s) 356
PspN4I GGNNCC 2 cut(s) 344, 375
PspPI GGNCC 2 cut(s) 293, 343
PsuI RGATCY 1 cut(s) 373
RsaI GTAC 1 cut(s) 133
RsaNI GTAC 1 cut(s) 132
RseI CAYNNNNRTG 1 cut(s) 499
SaqAI TTAA 2 cut(s) 60, 174
SatI GCNGC 1 cut(s) 33
Sau3AI GATC 6 cut(s) 104, 152, 184, 373, 384, 442
Sau96I GGNCC 2 cut(s) 293, 343
SchI GAGTC 1 cut(s) 266
ScrFI CCNGG 1 cut(s) 358
SetI ASST 5 cut(s) 72, 198, 298, 359, 371
SfaNI GCATC 1 cut(s) 457
SinI GGWCC 2 cut(s) 293, 343
SmiMI CAYNNNNRTG 1 cut(s) 499
Sse9I AATT 4 cut(s) 75, 212, 261, 452
SspMI CTAG 3 cut(s) 44, 303, 402
StyD4I CCNGG 1 cut(s) 356
StyI CCWWGG 1 cut(s) 494
TaaI ACNGT 1 cut(s) 433
TaiI ACGT 1 cut(s) 198
TaqI TCGA 1 cut(s) 511
TasI AATT 4 cut(s) 75, 212, 261, 452
Tru1I TTAA 2 cut(s) 60, 174
Tru9I TTAA 2 cut(s) 60, 174
TscAI CASTG 1 cut(s) 403
TseI GCWGC 1 cut(s) 32
TspDTI ATGAA 2 cut(s) 17, 205
TspRI CASTG 1 cut(s) 403
VpaK11BI GGWCC 2 cut(s) 293, 343
XapI RAATTY 1 cut(s) 212
XceI RCATGY 1 cut(s) 493
XspI CTAG 3 cut(s) 44, 303, 402
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.