Rmu_ssc0000432.1_g000071

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000432.1
OG0000668
Physical Location & Seq
Forward (+)
248352 .. 249767
1416 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_ssc0000432.1_g000071.1.cds

Sequence Viewer

Length: 1149 bp
atgatcatgggcggatgggaaaagagatcatcagacgtaccaccatccgatagtactcctcctactcaatcagaaagatcaaaatccacaaatgttgttagcattgatgatctccctgactctctattggttgaaatcctatgtcggtttcctagcaaggaattcgttttccaatgcaaatgtgtttctaagcattggctcaatctcatctcagttccgtatttgattggccgctttcgaaatcttcaaagatttcataagcaaacctcaatagctcgttctctgatagacagaaaaggggaggaattacccactcctagaacaacacagtcgtcatccaatctgctagctccactgttcaaaagactcgcgagtttccatgctttgaaaaaaaagccatatgtggtaggaacatctaatgatttagttttgtgctgccgaagtccacatgaccaacatgatttctacatttgcaatccatgcacaaggcaatggattgctcttcctcccaccactggacgtctcacgaatgtaccagttggattcatctgtgatcgctactatgaggaggaaaatagtactagtggtaccagtagtactgttaatattaaaggactacaggtgcaaggccgtaggcctaatggtgatgagactgttggcgatgatcattattaccaatttaatatctttgatgcagttgataagcttttagacaggccaagtatatgtgtgcaccgagggcgtctgcggtgggcctacaatagatgggactatgatcttattgtttgggagttaacaggtgatgatgatctcggatcagctgacaaattctatttgaaaaaagtgagtgtttatccagtaaccagtgaagttgctatatatggttatgctcttctagatttggatccgattgatgaggatagattttatgtaagagtgcgtactgactattttgcgtacaacgagtaccattttgcgtacaacgagttccatttcacgtataacatttgtacaagaaagtggtcaaaagtagctagcaccgagcatcttggtacaacatgcgaagactatcatctctttcgattggtgctcccattgtggccaacaccacttcgtagaccatcactagttgaagaattacccatatag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like

Protein Analysis

382

Amino Acids

44.24

Weight (kDa)

6.83

Isoelectric Point (pI)

53.18

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 523
Acc65I GGTACC 1 cut(s) 587
AccB1I GGYRCC 1 cut(s) 587
AccI GTMKAC 1 cut(s) 1117
AccII CGCG 1 cut(s) 371
AciI CCGC 3 cut(s) 12, 232, 748
AclWI GGATC 3 cut(s) 823, 899, 912
AcoI YGGCCR 2 cut(s) 229, 1100
AcsI RAATTY 2 cut(s) 161, 827
AcyI GRCGYC 2 cut(s) 520, 742
AfiI CCNNNNNNNGG 1 cut(s) 515
AgsI TTSAA 6 cut(s) 134, 248, 361, 388, 838, 1133
AhlI ACTAGT 2 cut(s) 581, 1126
AluBI AGCT 5 cut(s) 275, 350, 706, 821, 1034
AluI AGCT 5 cut(s) 275, 350, 706, 821, 1034
Alw21I GWGCWC 2 cut(s) 735, 1092
Alw26I GTCTC 2 cut(s) 527, 644
Alw44I GTGCAC 1 cut(s) 731
AlwI GGATC 3 cut(s) 823, 899, 912
AoxI GGCC 6 cut(s) 229, 628, 635, 716, 753, 1100
ApaLI GTGCAC 1 cut(s) 731
ApeKI GCWGC 1 cut(s) 435
ApoI RAATTY 2 cut(s) 161, 827
Asp718I GGTACC 1 cut(s) 587
AspS9I GGNCC 1 cut(s) 753
AsuHPI GGTGA 2 cut(s) 656, 812
AsuII TTCGAA 1 cut(s) 238
AsuNHI GCTAGC 2 cut(s) 346, 1034
BaeGI GKGCMC 1 cut(s) 735
BaeI ACNNNNGTAYC 2 cut(s) 516, 549
BalI TGGCCA 1 cut(s) 1102
BamHI GGATCC 1 cut(s) 904
BanI GGYRCC 1 cut(s) 587
BbsI GAAGAC 1 cut(s) 1071
Bbv12I GWGCWC 2 cut(s) 735, 1092
BbvI GCAGC 1 cut(s) 422
BccI CCATC 4 cut(s) 9, 52, 759, 1129
BceAI ACGGC 1 cut(s) 615
BcgI CGANNNNNNTGC 2 cut(s) 145, 179
BclI TGATCA 2 cut(s) 3, 664
BcoDI GTCTC 2 cut(s) 527, 644
BcuI ACTAGT 2 cut(s) 581, 1126
BfaI CTAG 7 cut(s) 153, 318, 347, 582, 896, 1035, 1127
BfmI CTRYAG 1 cut(s) 617
BisI GCNGC 2 cut(s) 232, 436
BlsI GCNGC 2 cut(s) 233, 437
BmcAI AGTACT 3 cut(s) 55, 580, 598
BmgT120I GGNCC 1 cut(s) 753
BmiI GGNNCC 2 cut(s) 589, 906
BmsI GCATC 2 cut(s) 682, 1054
BmtI GCTAGC 2 cut(s) 350, 1038
BpiI GAAGAC 1 cut(s) 1071
Bpu14I TTCGAA 1 cut(s) 238
BsaAI YACGTR 1 cut(s) 999
BsaBI GATNNNNATC 3 cut(s) 82, 807, 903
BsaHI GRCGYC 2 cut(s) 520, 742
BsaJI CCNNGG 1 cut(s) 736
Bsc4I CCNNNNNNNGG 1 cut(s) 515
Bse1I ACTGG 5 cut(s) 520, 536, 591, 857, 864
Bse3DI GCAATG 1 cut(s) 497
Bse8I GATNNNNATC 3 cut(s) 82, 807, 903
BseDI CCNNGG 1 cut(s) 736
BseGI GGATG 3 cut(s) 20, 44, 335
BseJI GATNNNNATC 3 cut(s) 82, 807, 903
BseLI CCNNNNNNNGG 1 cut(s) 515
BseMI GCAATG 1 cut(s) 497
BseMII CTCAG 1 cut(s) 225
BseNI ACTGG 5 cut(s) 520, 536, 591, 857, 864
BseRI GAGGAG 2 cut(s) 48, 581
BseSI GKGCMC 1 cut(s) 735
BseXI GCAGC 1 cut(s) 422
Bsh1236I CGCG 1 cut(s) 371
BshFI GGCC 6 cut(s) 231, 630, 637, 718, 755, 1102
BshNI GGYRCC 1 cut(s) 587
BsiHKAI GWGCWC 2 cut(s) 735, 1092
BslFI GGGAC 1 cut(s) 782
BslI CCNNNNNNNGG 1 cut(s) 515
BsmAI GTCTC 2 cut(s) 527, 644
BsmBI CGTCTC 1 cut(s) 527
BsmFI GGGAC 1 cut(s) 782
BsnI GGCC 6 cut(s) 231, 630, 637, 718, 755, 1102
Bsp119I TTCGAA 1 cut(s) 238
Bsp1286I GDGCHC 2 cut(s) 735, 1092
Bsp1407I TGTACA 1 cut(s) 1010
Bsp68I TCGCGA 1 cut(s) 371
BspACI CCGC 3 cut(s) 12, 232, 748
BspANI GGCC 6 cut(s) 231, 630, 637, 718, 755, 1102
BspCNI CTCAG 1 cut(s) 224
BspFNI CGCG 1 cut(s) 371
BspLI GGNNCC 2 cut(s) 589, 906
BspOI GCTAGC 2 cut(s) 350, 1038
BspPI GGATC 3 cut(s) 823, 899, 912
BspQI GCTCTTC 2 cut(s) 507, 897
BspT104I TTCGAA 1 cut(s) 238
BspT107I GGYRCC 1 cut(s) 587
BsrDI GCAATG 1 cut(s) 497
BsrGI TGTACA 1 cut(s) 1010
BsrI ACTGG 5 cut(s) 520, 536, 591, 857, 864
BssECI CCNNGG 1 cut(s) 736
BssNI GRCGYC 2 cut(s) 520, 742
Bst4CI ACNGT 4 cut(s) 330, 357, 601, 655
Bst6I CTCTTC 2 cut(s) 507, 897
BstACI GRCGYC 2 cut(s) 520, 742
BstAPI GCANNNNNTGC 1 cut(s) 480
BstAUI TGTACA 1 cut(s) 1010
BstBAI YACGTR 1 cut(s) 999
BstBI TTCGAA 1 cut(s) 238
BstC8I GCNNGC 2 cut(s) 348, 1036
BstDEI CTNAG 2 cut(s) 189, 211
BstF5I GGATG 3 cut(s) 20, 44, 335
BstFNI CGCG 1 cut(s) 371
BstMAI GTCTC 2 cut(s) 527, 644
BstMWI GCNNNNNNNGC 2 cut(s) 480, 739
BstNSI RCATGY 1 cut(s) 1062
BstSFI CTRYAG 1 cut(s) 617
BstSLI GKGCMC 1 cut(s) 735
BstUI CGCG 1 cut(s) 371
BstV1I GCAGC 1 cut(s) 422
BstV2I GAAGAC 1 cut(s) 1071
BstX2I RGATCY 1 cut(s) 904
BstYI RGATCY 1 cut(s) 904
BsuRI GGCC 6 cut(s) 231, 630, 637, 718, 755, 1102
BtgZI GCGATG 1 cut(s) 675
BtsCI GGATG 3 cut(s) 20, 44, 335
BtsIMutI CAGTG 3 cut(s) 353, 513, 871
BtuMI TCGCGA 1 cut(s) 371
Cac8I GCNNGC 2 cut(s) 348, 1036
Cfr13I GGNCC 1 cut(s) 753
CseI GACGC 1 cut(s) 731
CspCI CAANNNNNGTGG 2 cut(s) 76, 111
CviAII CATG 6 cut(s) 7, 380, 449, 458, 480, 1059
DdeI CTNAG 2 cut(s) 189, 211
EaeI YGGCCR 2 cut(s) 229, 1100
Eam1104I CTCTTC 2 cut(s) 507, 897
EarI CTCTTC 2 cut(s) 507, 897
EciI GGCGGA 1 cut(s) 27
Eco147I AGGCCT 1 cut(s) 637
EcoRI GAATTC 1 cut(s) 161
Esp3I CGTCTC 1 cut(s) 527
FaeI CATG 6 cut(s) 10, 383, 452, 461, 483, 1062
FaqI GGGAC 1 cut(s) 782
FatI CATG 6 cut(s) 6, 379, 448, 457, 479, 1058
FauNDI CATATG 1 cut(s) 400
FbaI TGATCA 2 cut(s) 3, 664
FblI GTMKAC 1 cut(s) 1117
Fnu4HI GCNGC 2 cut(s) 232, 436
FokI GGATG 3 cut(s) 27, 31, 322
Fsp4HI GCNGC 2 cut(s) 232, 436
FspBI CTAG 7 cut(s) 153, 318, 347, 582, 896, 1035, 1127
GluI GCNGC 2 cut(s) 232, 436
HaeIII GGCC 6 cut(s) 231, 630, 637, 718, 755, 1102
HgaI GACGC 1 cut(s) 731
Hin1I GRCGYC 2 cut(s) 520, 742
Hin1II CATG 6 cut(s) 10, 383, 452, 461, 483, 1062
HincII GTYRAC 1 cut(s) 795
HindII GTYRAC 1 cut(s) 795
HindIII AAGCTT 1 cut(s) 704
HinfI GANTC 3 cut(s) 119, 366, 543
HpaI GTTAAC 1 cut(s) 795
HphI GGTGA 2 cut(s) 656, 812
Hpy166II GTNNAC 4 cut(s) 446, 733, 795, 1118
Hpy188I TCNGA 6 cut(s) 34, 49, 73, 285, 815, 909
Hpy188III TCNNGA 3 cut(s) 370, 526, 896
Hpy8I GTNNAC 4 cut(s) 446, 733, 795, 1118
HpyCH4III ACNGT 4 cut(s) 330, 357, 601, 655
HpyCH4IV ACGT 3 cut(s) 36, 520, 998
HpyCH4V TGCA 6 cut(s) 177, 474, 483, 625, 695, 733
HpyF10VI GCNNNNNNNGC 2 cut(s) 480, 739
HpyF3I CTNAG 2 cut(s) 189, 211
HpySE526I ACGT 3 cut(s) 36, 520, 998
Hsp92I GRCGYC 2 cut(s) 520, 742
Hsp92II CATG 6 cut(s) 10, 383, 452, 461, 483, 1062
KpnI GGTACC 1 cut(s) 591
Ksp22I TGATCA 2 cut(s) 3, 664
KspAI GTTAAC 1 cut(s) 795
LguI GCTCTTC 2 cut(s) 507, 897
LmnI GCTCC 2 cut(s) 355, 1095
LpnPI CCDG 9 cut(s) 129, 501, 549, 604, 605, 700, 783, 870, 877
Lsp1109I GCAGC 1 cut(s) 422
LweI GCATC 2 cut(s) 682, 1054
MaeI CTAG 7 cut(s) 153, 318, 347, 582, 896, 1035, 1127
MaeII ACGT 3 cut(s) 36, 520, 998
MaeIII GTNAC 1 cut(s) 859
MboII GAAGA 5 cut(s) 236, 494, 884, 1076, 1145
MflI RGATCY 1 cut(s) 904
MhlI GDGCHC 2 cut(s) 735, 1092
MlsI TGGCCA 1 cut(s) 1102
MluCI AATT 5 cut(s) 161, 305, 677, 827, 1136
MluNI TGGCCA 1 cut(s) 1102
MlyI GAGTC 2 cut(s) 113, 360
MmeI TCCRAC 1 cut(s) 520
MnlI CCTC 8 cut(s) 69, 277, 295, 516, 559, 562, 731, 910
Mox20I TGGCCA 1 cut(s) 1102
MscI TGGCCA 1 cut(s) 1102
MseI TTAA 4 cut(s) 603, 609, 681, 794
Msp20I TGGCCA 1 cut(s) 1102
MspA1I CMGCKG 1 cut(s) 821
MvnI CGCG 1 cut(s) 371
MwoI GCNNNNNNNGC 2 cut(s) 480, 739
NdeI CATATG 1 cut(s) 400
NheI GCTAGC 2 cut(s) 346, 1034
NlaIII CATG 6 cut(s) 10, 383, 452, 461, 483, 1062
NlaIV GGNNCC 2 cut(s) 589, 906
NruI TCGCGA 1 cut(s) 371
NspI RCATGY 1 cut(s) 1062
NspV TTCGAA 1 cut(s) 238
PceI AGGCCT 1 cut(s) 637
PciSI GCTCTTC 2 cut(s) 507, 897
PfeI GAWTC 1 cut(s) 543
PkrI GCNGC 2 cut(s) 233, 437
PleI GAGTC 2 cut(s) 113, 360
PpsI GAGTC 2 cut(s) 113, 360
Ppu21I YACGTR 1 cut(s) 999
PspN4I GGNNCC 2 cut(s) 589, 906
PspPI GGNCC 1 cut(s) 753
PsrI GAACNNNNNNTAC 2 cut(s) 971, 1003
PsuI RGATCY 1 cut(s) 904
PvuII CAGCTG 1 cut(s) 821
RruI TCGCGA 1 cut(s) 371
SapI GCTCTTC 2 cut(s) 507, 897
SaqAI TTAA 4 cut(s) 603, 609, 681, 794
SatI GCNGC 2 cut(s) 232, 436
Sau96I GGNCC 1 cut(s) 753
ScaI AGTACT 3 cut(s) 55, 580, 598
SchI GAGTC 2 cut(s) 113, 360
SduI GDGCHC 2 cut(s) 735, 1092
SfaNI GCATC 2 cut(s) 682, 1054
SfcI CTRYAG 1 cut(s) 617
SfuI TTCGAA 1 cut(s) 238
SpeI ACTAGT 2 cut(s) 581, 1126
Sse9I AATT 5 cut(s) 161, 305, 677, 827, 1136
SseBI AGGCCT 1 cut(s) 637
SsiI CCGC 3 cut(s) 12, 232, 748
SspI AATATT 1 cut(s) 607
SspMI CTAG 7 cut(s) 153, 318, 347, 582, 896, 1035, 1127
StuI AGGCCT 1 cut(s) 637
TaaI ACNGT 4 cut(s) 330, 357, 601, 655
TaiI ACGT 3 cut(s) 39, 523, 1001
TaqI TCGA 2 cut(s) 238, 1081
TasI AATT 5 cut(s) 161, 305, 677, 827, 1136
TatI WGTACW 4 cut(s) 53, 578, 596, 1010
TauI GCSGC 1 cut(s) 234
TfiI GAWTC 1 cut(s) 543
Tru1I TTAA 4 cut(s) 603, 609, 681, 794
Tru9I TTAA 4 cut(s) 603, 609, 681, 794
TscAI CASTG 3 cut(s) 360, 520, 871
TseI GCWGC 1 cut(s) 435
TspDTI ATGAA 2 cut(s) 245, 535
TspGWI ACGGA 1 cut(s) 207
TspRI CASTG 3 cut(s) 360, 520, 871
VneI GTGCAC 1 cut(s) 731
XapI RAATTY 2 cut(s) 161, 827
XbaI TCTAGA 1 cut(s) 895
XceI RCATGY 1 cut(s) 1062
XmiI GTMKAC 1 cut(s) 1117
XspI CTAG 7 cut(s) 153, 318, 347, 582, 896, 1035, 1127
ZraI GACGTC 1 cut(s) 521
ZrmI AGTACT 3 cut(s) 55, 580, 598
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.