Rmu_ssc0000432.1_g000072

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000432.1
OG0000668
Physical Location & Seq
Forward (+)
253862 .. 255153
1292 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_ssc0000432.1_g000072.1.cds

Sequence Viewer

Length: 1194 bp
atgatctccctgactttctgttggttgaaatcctatgtcggcttcctagcaaggaattcgttttccaatgcaaatgtgtttccaagcattggctcaatctcatctcagatcgatccgtatttaattggccgctttctacatcttcaaagatttcataagcaatccccaatagcacgttctcttatacaaagaagaggggaggaatttcccactcctagaacgacacagtcgtcatccaatctgctagccccgctgttcaaaagactcatgagtttccacggtttggaagaaaagccatatgtggtaggaacatctaatgatttagttttatgctgccaaactctacattaccaagagcaacgtgatttctacatttgcaatccatgcacaaggcaaaggattgctcttcctcccaccacccgaagtcgcacgaatccaccagttggattcatctgtgatcgctactatgaggaggaagatagtactagtggtaccaatagtactgtattctcttttgagactggtgaatggagagagtcaattgtatcatccccacgaagcattaacattggagacactagcgttgcttacaatggaatgttgtattggacatatacggcatatgagcctcgtgaggagaccgggcttattaggctggatccgtttatgatcgacaatggcagtgctggtagtggtaggcctaatggtgatgagactggtgacgatgatcagtacaaattttatatctttgatgcagttgataagtttttagaaaggccatggatatgtgtgagccgagggcgtctgcaaatgtgggcgtactatagatgggactatgatgttattgtttgggagttaacaggtggtgatgatcttggatcagctgacaaattctatatgaaaaaagtgagtttttatccagtaacccgtgaagttgctatatatggttatgttcttctaggtttggatccgaatgatgaggatatcttttatgtagaagcgcatccttattactacccaatggactatttcatgtacaacattcgtacaagaaggtggtcaaaagtagctatagcaccaagtattttgaacagtggtggtcagacaatcgtctcgatctttaggttattggagctcccactgtggccaacaccacttcgtagactaccactggttgaagaattaccagtataa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like

Protein Analysis

397

Amino Acids

45.94

Weight (kDa)

5.55

Isoelectric Point (pI)

53.58

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 229
Acc65I GGTACC 1 cut(s) 491
AccB1I GGYRCC 1 cut(s) 491
AccB7I CCANNNNNTGG 3 cut(s) 89, 283, 443
AccI GTMKAC 1 cut(s) 1162
AciI CCGC 2 cut(s) 130, 251
AclWI GGATC 6 cut(s) 107, 653, 666, 886, 962, 975
AcoI YGGCCR 2 cut(s) 127, 1145
AcsI RAATTY 4 cut(s) 55, 203, 737, 890
AcyI GRCGYC 1 cut(s) 802
AfaI GTAC 7 cut(s) 484, 493, 502, 734, 821, 1037, 1048
AfiI CCNNNNNNNGG 3 cut(s) 89, 283, 443
AgsI TTSAA 5 cut(s) 28, 146, 259, 1090, 1178
AhlI ACTAGT 1 cut(s) 485
AjuI GAANNNNNNNTTGG 4 cut(s) 160, 192, 589, 621
AluBI AGCT 3 cut(s) 884, 1070, 1135
AluI AGCT 3 cut(s) 884, 1070, 1135
Alw21I GWGCWC 1 cut(s) 1137
Alw26I GTCTC 5 cut(s) 512, 567, 632, 707, 1117
AlwI GGATC 6 cut(s) 107, 653, 666, 886, 962, 975
AoxI GGCC 4 cut(s) 127, 698, 776, 1145
ApeKI GCWGC 1 cut(s) 333
ApoI RAATTY 4 cut(s) 55, 203, 737, 890
Asp718I GGTACC 1 cut(s) 491
AspLEI GCGC 1 cut(s) 1003
AsuC2I CCSGG 1 cut(s) 643
AsuHPI GGTGA 4 cut(s) 536, 719, 731, 878
AsuNHI GCTAGC 1 cut(s) 244
BalI TGGCCA 1 cut(s) 1147
BamHI GGATCC 2 cut(s) 658, 967
BanI GGYRCC 1 cut(s) 491
BanII GRGCYC 1 cut(s) 1137
BauI CACGAG 1 cut(s) 630
Bbv12I GWGCWC 1 cut(s) 1137
BbvI GCAGC 1 cut(s) 320
BccI CCATC 1 cut(s) 822
BceAI ACGGC 1 cut(s) 633
BcgI CGANNNNNNTGC 2 cut(s) 39, 73
BclI TGATCA 1 cut(s) 727
BcnI CCSGG 1 cut(s) 643
BcoDI GTCTC 5 cut(s) 512, 567, 632, 707, 1117
BcuI ACTAGT 1 cut(s) 485
BfaI CTAG 6 cut(s) 47, 216, 245, 486, 579, 959
BfmI CTRYAG 2 cut(s) 823, 1071
BisI GCNGC 2 cut(s) 130, 334
BlsI GCNGC 2 cut(s) 131, 335
BmcAI AGTACT 2 cut(s) 484, 502
Bme1390I CCNGG 1 cut(s) 643
BmiI GGNNCC 3 cut(s) 493, 660, 969
BmrFI CCNGG 1 cut(s) 643
BmsI GCATC 2 cut(s) 742, 1012
BmtI GCTAGC 1 cut(s) 248
BoxI GACNNNNGTC 1 cut(s) 1109
BpuMI CCSGG 1 cut(s) 643
Bsa29I ATCGAT 1 cut(s) 111
BsaHI GRCGYC 1 cut(s) 802
BsaI GGTCTC 1 cut(s) 632
BsaJI CCNNGG 3 cut(s) 277, 779, 796
Bsc4I CCNNNNNNNGG 3 cut(s) 89, 283, 443
Bse1I ACTGG 6 cut(s) 440, 526, 721, 920, 1176, 1187
BseCI ATCGAT 1 cut(s) 111
BseDI CCNNGG 3 cut(s) 277, 779, 796
BseGI GGATG 3 cut(s) 233, 548, 1003
BseLI CCNNNNNNNGG 3 cut(s) 89, 283, 443
BseMII CTCAG 1 cut(s) 119
BseNI ACTGG 6 cut(s) 440, 526, 721, 920, 1176, 1187
BseRI GAGGAG 2 cut(s) 485, 650
BseXI GCAGC 1 cut(s) 320
BshFI GGCC 4 cut(s) 129, 700, 778, 1147
BshNI GGYRCC 1 cut(s) 491
BshVI ATCGAT 1 cut(s) 111
BsiHKAI GWGCWC 1 cut(s) 1137
BsiSI CCGG 1 cut(s) 642
BslFI GGGAC 1 cut(s) 845
BslI CCNNNNNNNGG 3 cut(s) 89, 283, 443
BsmAI GTCTC 5 cut(s) 512, 567, 632, 707, 1117
BsmBI CGTCTC 1 cut(s) 1117
BsmFI GGGAC 1 cut(s) 845
BsnI GGCC 4 cut(s) 129, 700, 778, 1147
Bso31I GGTCTC 1 cut(s) 632
Bsp1286I GDGCHC 1 cut(s) 1137
Bsp1407I TGTACA 1 cut(s) 1035
Bsp19I CCATGG 1 cut(s) 779
BspACI CCGC 2 cut(s) 130, 251
BspANI GGCC 4 cut(s) 129, 700, 778, 1147
BspCNI CTCAG 1 cut(s) 118
BspDI ATCGAT 1 cut(s) 111
BspHI TCATGA 1 cut(s) 267
BspLI GGNNCC 3 cut(s) 493, 660, 969
BspOI GCTAGC 1 cut(s) 248
BspPI GGATC 6 cut(s) 107, 653, 666, 886, 962, 975
BspQI GCTCTTC 1 cut(s) 411
BspT107I GGYRCC 1 cut(s) 491
BspTNI GGTCTC 1 cut(s) 632
BsrGI TGTACA 1 cut(s) 1035
BsrI ACTGG 6 cut(s) 440, 526, 721, 920, 1176, 1187
BssECI CCNNGG 3 cut(s) 277, 779, 796
BssNI GRCGYC 1 cut(s) 802
BssSI CACGAG 1 cut(s) 630
BssT1I CCWWGG 1 cut(s) 779
Bst2BI CACGAG 1 cut(s) 630
Bst4CI ACNGT 5 cut(s) 228, 281, 505, 1094, 1143
Bst6I CTCTTC 2 cut(s) 187, 411
BstACI GRCGYC 1 cut(s) 802
BstAPI GCANNNNNTGC 1 cut(s) 384
BstAUI TGTACA 1 cut(s) 1035
BstC8I GCNNGC 1 cut(s) 246
BstDEI CTNAG 1 cut(s) 105
BstDSI CCRYGG 2 cut(s) 277, 779
BstF5I GGATG 3 cut(s) 233, 548, 1003
BstHHI GCGC 1 cut(s) 1003
BstMAI GTCTC 5 cut(s) 512, 567, 632, 707, 1117
BstMWI GCNNNNNNNGC 3 cut(s) 250, 384, 652
BstPAI GACNNNNGTC 1 cut(s) 1109
BstSCI CCNGG 1 cut(s) 641
BstSFI CTRYAG 2 cut(s) 823, 1071
BstV1I GCAGC 1 cut(s) 320
BstX2I RGATCY 2 cut(s) 658, 967
BstYI RGATCY 2 cut(s) 658, 967
Bsu15I ATCGAT 1 cut(s) 111
BsuRI GGCC 4 cut(s) 129, 700, 778, 1147
BsuTUI ATCGAT 1 cut(s) 111
BtgI CCRYGG 2 cut(s) 277, 779
BtsCI GGATG 3 cut(s) 233, 548, 1003
BtsI GCAGTG 1 cut(s) 688
BtsIMutI CAGTG 4 cut(s) 688, 1099, 1139, 1169
Cac8I GCNNGC 1 cut(s) 246
CciI TCATGA 1 cut(s) 267
CfoI GCGC 1 cut(s) 1003
ClaI ATCGAT 1 cut(s) 111
CseI GACGC 1 cut(s) 791
Csp6I GTAC 7 cut(s) 483, 492, 501, 733, 820, 1036, 1047
CviAII CATG 4 cut(s) 268, 384, 780, 1033
CviQI GTAC 7 cut(s) 483, 492, 501, 733, 820, 1036, 1047
DdeI CTNAG 1 cut(s) 105
DrdI GACNNNNNNGTC 1 cut(s) 229
DseDI GACNNNNNNGTC 1 cut(s) 229
EaeI YGGCCR 2 cut(s) 127, 1145
Eam1104I CTCTTC 2 cut(s) 187, 411
EarI CTCTTC 2 cut(s) 187, 411
Ecl136II GAGCTC 1 cut(s) 1135
Eco130I CCWWGG 1 cut(s) 779
Eco147I AGGCCT 1 cut(s) 700
Eco24I GRGCYC 1 cut(s) 1137
Eco31I GGTCTC 1 cut(s) 632
Eco32I GATATC 1 cut(s) 985
Eco53kI GAGCTC 1 cut(s) 1135
EcoICRI GAGCTC 1 cut(s) 1135
EcoRI GAATTC 1 cut(s) 55
EcoRV GATATC 1 cut(s) 985
EcoT14I CCWWGG 1 cut(s) 779
EcoT38I GRGCYC 1 cut(s) 1137
ErhI CCWWGG 1 cut(s) 779
Esp3I CGTCTC 1 cut(s) 1117
FaeI CATG 4 cut(s) 271, 387, 783, 1036
FaqI GGGAC 1 cut(s) 845
FatI CATG 4 cut(s) 267, 383, 779, 1032
FauI CCCGC 1 cut(s) 258
FauNDI CATATG 2 cut(s) 298, 622
FbaI TGATCA 1 cut(s) 727
FblI GTMKAC 1 cut(s) 1162
Fnu4HI GCNGC 2 cut(s) 130, 334
FokI GGATG 3 cut(s) 220, 535, 990
FriOI GRGCYC 1 cut(s) 1137
Fsp4HI GCNGC 2 cut(s) 130, 334
FspBI CTAG 6 cut(s) 47, 216, 245, 486, 579, 959
GlaI GCGC 1 cut(s) 1002
GluI GCNGC 2 cut(s) 130, 334
HaeIII GGCC 4 cut(s) 129, 700, 778, 1147
HapII CCGG 1 cut(s) 642
HgaI GACGC 1 cut(s) 791
HhaI GCGC 1 cut(s) 1003
Hin1I GRCGYC 1 cut(s) 802
Hin1II CATG 4 cut(s) 271, 387, 783, 1036
Hin6I GCGC 1 cut(s) 1001
HinP1I GCGC 1 cut(s) 1001
HincII GTYRAC 1 cut(s) 858
HindII GTYRAC 1 cut(s) 858
HinfI GANTC 4 cut(s) 264, 433, 447, 536
HpaI GTTAAC 1 cut(s) 858
HpaII CCGG 1 cut(s) 642
HphI GGTGA 4 cut(s) 536, 719, 731, 878
Hpy166II GTNNAC 2 cut(s) 858, 1163
Hpy188I TCNGA 3 cut(s) 108, 972, 1104
Hpy188III TCNNGA 3 cut(s) 268, 632, 1114
Hpy8I GTNNAC 2 cut(s) 858, 1163
HpyAV CCTTC 1 cut(s) 1047
HpyCH4III ACNGT 5 cut(s) 228, 281, 505, 1094, 1143
HpyCH4IV ACGT 2 cut(s) 175, 361
HpyCH4V TGCA 5 cut(s) 71, 378, 387, 755, 808
HpyF10VI GCNNNNNNNGC 3 cut(s) 250, 384, 652
HpyF3I CTNAG 1 cut(s) 105
HpySE526I ACGT 2 cut(s) 175, 361
Hsp92I GRCGYC 1 cut(s) 802
Hsp92II CATG 4 cut(s) 271, 387, 783, 1036
HspAI GCGC 1 cut(s) 1001
KpnI GGTACC 1 cut(s) 495
Ksp22I TGATCA 1 cut(s) 727
KspAI GTTAAC 1 cut(s) 858
LguI GCTCTTC 1 cut(s) 411
LmnI GCTCC 2 cut(s) 1132, 1140
Lsp1109I GCAGC 1 cut(s) 320
LweI GCATC 2 cut(s) 742, 1012
MaeI CTAG 6 cut(s) 47, 216, 245, 486, 579, 959
MaeII ACGT 2 cut(s) 175, 361
MaeIII GTNAC 2 cut(s) 719, 922
MboII GAAGA 7 cut(s) 134, 204, 299, 398, 488, 947, 1190
MfeI CAATTG 1 cut(s) 540
MflI RGATCY 2 cut(s) 658, 967
MhlI GDGCHC 1 cut(s) 1137
MlsI TGGCCA 1 cut(s) 1147
MluCI AATT 7 cut(s) 55, 123, 203, 540, 737, 890, 1181
MluNI TGGCCA 1 cut(s) 1147
MlyI GAGTC 2 cut(s) 258, 545
MmeI TCCRAC 1 cut(s) 424
MnlI CCTC 9 cut(s) 188, 193, 420, 463, 466, 628, 639, 791, 973
Mox20I TGGCCA 1 cut(s) 1147
MscI TGGCCA 1 cut(s) 1147
MseI TTAA 3 cut(s) 122, 564, 857
MslI CAYNNNNRTG 1 cut(s) 784
Msp20I TGGCCA 1 cut(s) 1147
MspA1I CMGCKG 2 cut(s) 253, 884
MspI CCGG 1 cut(s) 642
MspR9I CCNGG 1 cut(s) 643
MunI CAATTG 1 cut(s) 540
MwoI GCNNNNNNNGC 3 cut(s) 250, 384, 652
NciI CCSGG 1 cut(s) 643
NcoI CCATGG 1 cut(s) 779
NdeI CATATG 2 cut(s) 298, 622
NheI GCTAGC 1 cut(s) 244
NlaIII CATG 4 cut(s) 271, 387, 783, 1036
NlaIV GGNNCC 3 cut(s) 493, 660, 969
NmeAIII GCCGAG 1 cut(s) 821
NmuCI GTSAC 1 cut(s) 719
PagI TCATGA 1 cut(s) 267
PceI AGGCCT 1 cut(s) 700
PciSI GCTCTTC 1 cut(s) 411
PcsI WCGNNNNNNNCGW 1 cut(s) 227
PfeI GAWTC 2 cut(s) 433, 447
PflFI GACNNNGTC 1 cut(s) 226
PflMI CCANNNNNTGG 3 cut(s) 89, 283, 443
PkrI GCNGC 2 cut(s) 131, 335
PleI GAGTC 2 cut(s) 258, 544
PpsI GAGTC 2 cut(s) 258, 544
PshAI GACNNNNGTC 1 cut(s) 1109
Psp124BI GAGCTC 1 cut(s) 1137
PspN4I GGNNCC 3 cut(s) 493, 660, 969
PsuI RGATCY 2 cut(s) 658, 967
PsyI GACNNNGTC 1 cut(s) 226
PvuII CAGCTG 1 cut(s) 884
RsaI GTAC 7 cut(s) 484, 493, 502, 734, 821, 1037, 1048
RsaNI GTAC 7 cut(s) 483, 492, 501, 733, 820, 1036, 1047
RseI CAYNNNNRTG 1 cut(s) 784
SacI GAGCTC 1 cut(s) 1137
SapI GCTCTTC 1 cut(s) 411
SaqAI TTAA 3 cut(s) 122, 564, 857
SatI GCNGC 2 cut(s) 130, 334
ScaI AGTACT 2 cut(s) 484, 502
SchI GAGTC 2 cut(s) 258, 545
ScrFI CCNGG 1 cut(s) 643
SduI GDGCHC 1 cut(s) 1137
SetI ASST 9 cut(s) 178, 364, 865, 886, 964, 1058, 1072, 1127, 1137
SfaNI GCATC 2 cut(s) 742, 1012
SfcI CTRYAG 2 cut(s) 823, 1071
SmiMI CAYNNNNRTG 1 cut(s) 784
SpeI ACTAGT 1 cut(s) 485
Sse9I AATT 7 cut(s) 55, 123, 203, 540, 737, 890, 1181
SseBI AGGCCT 1 cut(s) 700
SsiI CCGC 2 cut(s) 130, 251
SspMI CTAG 6 cut(s) 47, 216, 245, 486, 579, 959
SstI GAGCTC 1 cut(s) 1137
StuI AGGCCT 1 cut(s) 700
StyD4I CCNGG 1 cut(s) 641
StyI CCWWGG 1 cut(s) 779
TaaI ACNGT 5 cut(s) 228, 281, 505, 1094, 1143
TaiI ACGT 2 cut(s) 178, 364
TaqI TCGA 3 cut(s) 111, 672, 1115
TasI AATT 7 cut(s) 55, 123, 203, 540, 737, 890, 1181
TatI WGTACW 4 cut(s) 482, 500, 732, 1035
TauI GCSGC 1 cut(s) 132
TfiI GAWTC 2 cut(s) 433, 447
Tru1I TTAA 3 cut(s) 122, 564, 857
Tru9I TTAA 3 cut(s) 122, 564, 857
TscAI CASTG 4 cut(s) 688, 1099, 1146, 1176
TseFI GTSAC 1 cut(s) 719
TseI GCWGC 1 cut(s) 333
Tsp45I GTSAC 1 cut(s) 719
TspDTI ATGAA 4 cut(s) 143, 439, 914, 1021
TspGWI ACGGA 2 cut(s) 105, 651
TspRI CASTG 4 cut(s) 688, 1099, 1146, 1176
Tth111I GACNNNGTC 1 cut(s) 226
Van91I CCANNNNNTGG 3 cut(s) 89, 283, 443
XapI RAATTY 4 cut(s) 55, 203, 737, 890
XmiI GTMKAC 1 cut(s) 1162
XspI CTAG 6 cut(s) 47, 216, 245, 486, 579, 959
ZrmI AGTACT 2 cut(s) 484, 502
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.